Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0343810 | XP_003609812.1 | 93.220 | 177 | 8 | 1 | 1 | 177 | 1 | 173 | 9.61e-114 | 330 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0343810 | sp|F4HWQ8|CVIF1_ARATH | 46.479 | 142 | 76 | 0 | 32 | 173 | 21 | 162 | 2.08e-42 | 143 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0343810 | G7JP02 | 93.220 | 177 | 8 | 1 | 1 | 177 | 1 | 173 | 4.59e-114 | 330 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002540 | Msa0343810 | 0.814964 | 1.178367e-51 | -8.615850e-47 |
| Msa0008340 | Msa0343810 | 0.820516 | 6.580836e-53 | -8.615850e-47 |
| Msa0008410 | Msa0343810 | 0.805781 | 1.131207e-49 | -8.615850e-47 |
| Msa0015300 | Msa0343810 | 0.808476 | 3.039909e-50 | -8.615850e-47 |
| Msa0041970 | Msa0343810 | 0.815686 | 8.139261e-52 | -8.615850e-47 |
| Msa0044710 | Msa0343810 | 0.807861 | 4.110893e-50 | -8.615850e-47 |
| Msa0047980 | Msa0343810 | 0.809617 | 1.732245e-50 | -8.615850e-47 |
| Msa0050730 | Msa0343810 | 0.817188 | 3.753368e-52 | -8.615850e-47 |
| Msa0050760 | Msa0343810 | 0.819611 | 1.060529e-52 | -8.615850e-47 |
| Msa0064350 | Msa0343810 | 0.838297 | 3.127564e-57 | -8.615850e-47 |
| Msa0094920 | Msa0343810 | 0.805688 | 1.182917e-49 | -8.615850e-47 |
| Msa0105420 | Msa0343810 | 0.824952 | 6.108425e-54 | -8.615850e-47 |
| Msa0129060 | Msa0343810 | 0.808486 | 3.025527e-50 | -8.615850e-47 |
| Msa0143080 | Msa0343810 | 0.800952 | 1.132121e-48 | -8.615850e-47 |
| Msa0148100 | Msa0343810 | 0.804666 | 1.935930e-49 | -8.615850e-47 |
| Msa0149390 | Msa0343810 | 0.805241 | 1.467686e-49 | -8.615850e-47 |
| Msa0164360 | Msa0343810 | 0.800577 | 1.349959e-48 | -8.615850e-47 |
| Msa0184020 | Msa0343810 | 0.804855 | 1.767925e-49 | -8.615850e-47 |
| Msa0185480 | Msa0343810 | 0.817193 | 3.743580e-52 | -8.615850e-47 |
| Msa0188630 | Msa0343810 | 0.829328 | 5.474207e-55 | -8.615850e-47 |
| Msa0191050 | Msa0343810 | 0.807650 | 4.557542e-50 | -8.615850e-47 |
| Msa0204980 | Msa0343810 | 0.801272 | 9.739082e-49 | -8.615850e-47 |
| Msa0205850 | Msa0343810 | 0.809049 | 2.292600e-50 | -8.615850e-47 |
| Msa0230770 | Msa0343810 | 0.808695 | 2.729689e-50 | -8.615850e-47 |
| Msa0233350 | Msa0343810 | 0.813207 | 2.876256e-51 | -8.615850e-47 |
| Msa0236980 | Msa0343810 | 0.803201 | 3.902940e-49 | -8.615850e-47 |
| Msa0260210 | Msa0343810 | 0.802119 | 6.525209e-49 | -8.615850e-47 |
| Msa0282250 | Msa0343810 | 0.800521 | 1.386229e-48 | -8.615850e-47 |
| Msa0296760 | Msa0343810 | 0.819384 | 1.194969e-52 | -8.615850e-47 |
| Msa0304290 | Msa0343810 | 0.806053 | 9.915703e-50 | -8.615850e-47 |
| Msa0304330 | Msa0343810 | 0.804856 | 1.766834e-49 | -8.615850e-47 |
| Msa0311700 | Msa0343810 | 0.827093 | 1.892483e-54 | -8.615850e-47 |
| Msa0316850 | Msa0343810 | 0.809865 | 1.532044e-50 | -8.615850e-47 |
| Msa0328210 | Msa0343810 | 0.817644 | 2.963619e-52 | -8.615850e-47 |
| Msa0343810 | Msa0355270 | 0.803875 | 2.828475e-49 | -8.615850e-47 |
| Msa0343810 | Msa0356960 | 0.804570 | 2.027269e-49 | -8.615850e-47 |
| Msa0343810 | Msa0376040 | 0.810613 | 1.056662e-50 | -8.615850e-47 |
| Msa0343810 | Msa0380040 | 0.806265 | 8.948027e-50 | -8.615850e-47 |
| Msa0343810 | Msa0392940 | 0.811447 | 6.968497e-51 | -8.615850e-47 |
| Msa0343810 | Msa0393710 | 0.806092 | 9.728646e-50 | -8.615850e-47 |
| Msa0343810 | Msa0426330 | 0.826924 | 2.076564e-54 | -8.615850e-47 |
| Msa0343810 | Msa0449820 | 0.801109 | 1.051476e-48 | -8.615850e-47 |
| Msa0343810 | Msa0449830 | 0.836986 | 6.783136e-57 | -8.615850e-47 |
| Msa0343810 | Msa0516040 | 0.804064 | 2.584178e-49 | -8.615850e-47 |
| Msa0343810 | Msa0535450 | 0.806761 | 7.029304e-50 | -8.615850e-47 |
| Msa0343810 | Msa0551950 | 0.810449 | 1.146456e-50 | -8.615850e-47 |
| Msa0343810 | Msa0552810 | 0.801079 | 1.066391e-48 | -8.615850e-47 |
| Msa0343810 | Msa0578970 | 0.810323 | 1.220641e-50 | -8.615850e-47 |
| Msa0343810 | Msa0585900 | 0.829890 | 3.995389e-55 | -8.615850e-47 |
| Msa0343810 | Msa0587690 | 0.801968 | 7.010328e-49 | -8.615850e-47 |
| Msa0343810 | Msa0591770 | 0.808115 | 3.629586e-50 | -8.615850e-47 |
| Msa0343810 | Msa0610590 | 0.819164 | 1.340795e-52 | -8.615850e-47 |
| Msa0343810 | Msa0616020 | 0.801208 | 1.003611e-48 | -8.615850e-47 |
| Msa0343810 | Msa0651190 | 0.809438 | 1.892034e-50 | -8.615850e-47 |
| Msa0343810 | Msa0663900 | 0.813370 | 2.648682e-51 | -8.615850e-47 |
| Msa0343810 | Msa0669850 | 0.811663 | 6.256340e-51 | -8.615850e-47 |
| Msa0343810 | Msa0680240 | 0.826161 | 3.157864e-54 | -8.615850e-47 |
| Msa0343810 | Msa0684500 | 0.804503 | 2.094136e-49 | -8.615850e-47 |
| Msa0343810 | Msa0687630 | 0.810413 | 1.167331e-50 | -8.615850e-47 |
| Msa0343810 | Msa0690160 | 0.839352 | 1.668281e-57 | -8.615850e-47 |
| Msa0343810 | Msa0694250 | 0.811675 | 6.217684e-51 | -8.615850e-47 |
| Msa0343810 | Msa0711490 | 0.800367 | 1.490112e-48 | -8.615850e-47 |
| Msa0343810 | Msa0713820 | 0.826665 | 2.394359e-54 | -8.615850e-47 |
| Msa0343810 | Msa0743260 | 0.801943 | 7.093009e-49 | -8.615850e-47 |
| Msa0343810 | Msa0756060 | 0.804220 | 2.398827e-49 | -8.615850e-47 |
| Msa0343810 | Msa0791100 | 0.804918 | 1.715212e-49 | -8.615850e-47 |
| Msa0343810 | Msa0792090 | 0.801000 | 1.106897e-48 | -8.615850e-47 |
| Msa0343810 | Msa0797750 | 0.827184 | 1.799759e-54 | -8.615850e-47 |
| Msa0343810 | Msa0835280 | 0.813915 | 2.010140e-51 | -8.615850e-47 |
| Msa0343810 | Msa0837660 | 0.831211 | 1.897926e-55 | -8.615850e-47 |
| Msa0343810 | Msa0877410 | 0.803655 | 3.142901e-49 | -8.615850e-47 |
| Msa0343810 | Msa0899230 | 0.810321 | 1.221746e-50 | -8.615850e-47 |
| Msa0343810 | Msa0900630 | 0.807168 | 5.766302e-50 | -8.615850e-47 |
| Msa0343810 | Msa0908170 | 0.812425 | 4.266256e-51 | -8.615850e-47 |
| Msa0343810 | Msa0910980 | -0.807913 | 4.006774e-50 | -8.615850e-47 |
| Msa0343810 | Msa0922540 | 0.804932 | 1.703593e-49 | -8.615850e-47 |
| Msa0343810 | Msa0944770 | 0.808637 | 2.809072e-50 | -8.615850e-47 |
| Msa0343810 | Msa0960160 | 0.805893 | 1.071568e-49 | -8.615850e-47 |
| Msa0343810 | Msa0993530 | 0.804381 | 2.219775e-49 | -8.615850e-47 |
| Msa0343810 | Msa0996080 | 0.807301 | 5.405238e-50 | -8.615850e-47 |
| Msa0343810 | Msa1089840 | 0.803289 | 3.743172e-49 | -8.615850e-47 |
| Msa0343810 | Msa1092090 | 0.801770 | 7.696554e-49 | -8.615850e-47 |
| Msa0343810 | Msa1097840 | 0.809205 | 2.122852e-50 | -8.615850e-47 |
| Msa0343810 | Msa1165870 | 0.801073 | 1.069468e-48 | -8.615850e-47 |
| Msa0343810 | Msa1199560 | 0.814065 | 1.862517e-51 | -8.615850e-47 |
| Msa0343810 | Msa1201120 | 0.801346 | 9.403718e-49 | -8.615850e-47 |
| Msa0343810 | Msa1218960 | 0.993778 | 3.713399e-202 | -8.615850e-47 |
| Msa0343810 | Msa1233890 | 0.801760 | 7.735122e-49 | -8.615850e-47 |
| Msa0343810 | Msa1267260 | 0.803124 | 4.048082e-49 | -8.615850e-47 |
| Msa0343810 | Msa1269300 | 0.804213 | 2.406219e-49 | -8.615850e-47 |
| Msa0343810 | Msa1282920 | 0.828686 | 7.833345e-55 | -8.615850e-47 |
| Msa0343810 | Msa1304820 | 0.816992 | 4.154386e-52 | -8.615850e-47 |
| Msa0343810 | Msa1328700 | 0.809529 | 1.808676e-50 | -8.615850e-47 |
| Msa0343810 | Msa1340020 | 0.815974 | 7.021868e-52 | -8.615850e-47 |
| Msa0343810 | Msa1343820 | 0.837193 | 6.006778e-57 | -8.615850e-47 |
| Msa0343810 | Msa1365150 | 0.805164 | 1.523496e-49 | -8.615850e-47 |
| Msa0343810 | Msa1367880 | 0.805539 | 1.271498e-49 | -8.615850e-47 |
| Msa0343810 | Msa1372580 | 0.803492 | 3.397934e-49 | -8.615850e-47 |
| Msa0343810 | Msa1404010 | 0.817302 | 3.538788e-52 | -8.615850e-47 |
| Msa0343810 | Msa1404190 | 0.807312 | 5.374845e-50 | -8.615850e-47 |
| Msa0343810 | Msa1404730 | 0.802056 | 6.722560e-49 | -8.615850e-47 |
| Msa0343810 | Msa1423450 | 0.807027 | 6.178128e-50 | -8.615850e-47 |
| Msa0343810 | Msa1440600 | 0.819960 | 8.825398e-53 | -8.615850e-47 |
| Msa0343810 | Msa1448510 | 0.805041 | 1.616397e-49 | -8.615850e-47 |
| Msa0343810 | Msa1452470 | 0.808722 | 2.693717e-50 | -8.615850e-47 |
| Msa0343810 | Msa1453080 | 0.808389 | 3.172082e-50 | -8.615850e-47 |
| Msa0343810 | Msa1461280 | 0.812233 | 4.700498e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0343810 | MtrunA17_Chr4g0068711 | 93.220 | 177 | 8 | 1 | 1 | 177 | 1 | 173 | 8.84e-118 | 330 |
| Msa0343810 | MtrunA17_Chr4g0068731 | 70.286 | 175 | 45 | 3 | 1 | 172 | 1 | 171 | 7.23e-80 | 234 |
| Msa0343810 | MtrunA17_Chr4g0068791 | 54.749 | 179 | 75 | 4 | 1 | 177 | 1 | 175 | 9.65e-62 | 189 |
| Msa0343810 | MtrunA17_Chr4g0068751 | 51.648 | 182 | 83 | 1 | 1 | 177 | 1 | 182 | 5.06e-59 | 182 |
| Msa0343810 | MtrunA17_Chr4g0001591 | 50.926 | 108 | 51 | 2 | 72 | 177 | 1 | 108 | 2.17e-26 | 97.1 |
| Msa0343810 | MtrunA17_Chr2g0294121 | 36.842 | 171 | 96 | 4 | 19 | 177 | 6 | 176 | 6.19e-23 | 90.1 |
| Msa0343810 | MtrunA17_Chr4g0068701 | 36.076 | 158 | 96 | 3 | 25 | 177 | 20 | 177 | 8.34e-21 | 84.3 |
| Msa0343810 | MtrunA17_Chr4g0068651 | 28.415 | 183 | 121 | 5 | 1 | 177 | 1 | 179 | 8.85e-20 | 82.0 |
| Msa0343810 | MtrunA17_Chr4g0068681 | 31.973 | 147 | 96 | 2 | 35 | 177 | 39 | 185 | 2.08e-15 | 72.8 |
| Msa0343810 | MtrunA17_Chr4g0068681 | 31.724 | 145 | 96 | 2 | 35 | 177 | 230 | 373 | 1.68e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0343810 | AT1G47960.2 | 47.260 | 146 | 77 | 0 | 32 | 177 | 21 | 166 | 1.75e-46 | 150 |
| Msa0343810 | AT1G47960.1 | 46.479 | 142 | 76 | 0 | 32 | 173 | 21 | 162 | 2.12e-43 | 143 |
| Msa0343810 | AT3G17152.1 | 30.286 | 175 | 120 | 1 | 4 | 176 | 1 | 175 | 5.27e-17 | 74.7 |
| Msa0343810 | AT3G17150.1 | 31.944 | 144 | 94 | 2 | 37 | 177 | 39 | 181 | 5.86e-15 | 69.7 |
| Msa0343810 | AT5G64620.1 | 38.776 | 98 | 56 | 3 | 15 | 108 | 8 | 105 | 9.55e-11 | 58.2 |
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATGCAATTTGCATAATT+TGG | 0.146977 | 3_1:+2252806 | None:intergenic |
| GCACCATCTTCTGCAAATTT+AGG | 0.160091 | 3_1:+2252557 | None:intergenic |
| ATCAAATTAGCATTGGATTT+TGG | 0.186303 | 3_1:+2252851 | None:intergenic |
| TTGAGAGCTGCAACAGATTT+TGG | 0.208499 | 3_1:+2252590 | None:intergenic |
| ACATCTGCATCAGAGCTTCT+TGG | 0.293970 | 3_1:+2252767 | None:intergenic |
| CAAAATCAATCAGTTGATTA+AGG | 0.305258 | 3_1:-2252684 | Msa0343810:CDS |
| TGGTAGATACAAAGCAATTT+TGG | 0.313317 | 3_1:-2252624 | Msa0343810:CDS |
| AATGCTAATGCTAATGCTAA+TGG | 0.350974 | 3_1:+2252923 | None:intergenic |
| GTTTAAGGCAGTGTTTGCTT+TGG | 0.395895 | 3_1:+2252706 | None:intergenic |
| AGCTCTGATGCAGATGTAAC+AGG | 0.425806 | 3_1:-2252761 | Msa0343810:CDS |
| ACTTGTGAGAATGGCTTCAA+TGG | 0.435357 | 3_1:-2252509 | Msa0343810:CDS |
| AGCTTCTTGGATCAGATTTG+AGG | 0.439581 | 3_1:+2252780 | None:intergenic |
| GAAGCCACTACTTGTGAGAA+TGG | 0.448649 | 3_1:-2252518 | Msa0343810:CDS |
| TTGCTGTATCAAATTAGCAT+TGG | 0.473617 | 3_1:+2252844 | None:intergenic |
| TAAGGAGAGCCATGATCAAA+AGG | 0.474578 | 3_1:-2252666 | Msa0343810:CDS |
| CTTGTGAGAATGGCTTCAAT+GGG | 0.531153 | 3_1:-2252508 | Msa0343810:CDS |
| GATCCTAAATTTGCAGAAGA+TGG | 0.542535 | 3_1:-2252560 | Msa0343810:CDS |
| GAAGCTTTGAATTCATGTGC+TGG | 0.550109 | 3_1:-2252644 | Msa0343810:CDS |
| AACAGGTCTAGCACTAATCA+TGG | 0.552410 | 3_1:-2252744 | Msa0343810:CDS |
| GAAGCCATTCTCACAAGTAG+TGG | 0.571548 | 3_1:+2252514 | None:intergenic |
| TCTGTTGCAGCTCTCAAGCA+AGG | 0.585277 | 3_1:-2252584 | Msa0343810:CDS |
| GCAATATTCTCATCAGAGAG+TGG | 0.649907 | 3_1:+2252479 | None:intergenic |
| AGGTCTAGCACTAATCATGG+TGG | 0.723735 | 3_1:-2252741 | Msa0343810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCAAATTAGCATTGGATTT+TGG | + | chr3_1:2252517-2252536 | None:intergenic | 25.0% |
| ! | TGAATGCAATTTGCATAATT+TGG | + | chr3_1:2252562-2252581 | None:intergenic | 25.0% |
| !!! | CAACTGATTGATTTTGTTTA+AGG | + | chr3_1:2252677-2252696 | None:intergenic | 25.0% |
| ! | CAAAATCAATCAGTTGATTA+AGG | - | chr3_1:2252681-2252700 | Msa0343810:CDS | 25.0% |
| AATGCTAATGCTAATGCTAA+TGG | + | chr3_1:2252445-2252464 | None:intergenic | 30.0% | |
| !! | AATTAGCATTGGATTTTGGA+TGG | + | chr3_1:2252513-2252532 | None:intergenic | 30.0% |
| TTGCTGTATCAAATTAGCAT+TGG | + | chr3_1:2252524-2252543 | None:intergenic | 30.0% | |
| ! | TGGTAGATACAAAGCAATTT+TGG | - | chr3_1:2252741-2252760 | Msa0343810:CDS | 30.0% |
| ! | TCAAAGCTTCCTTTTGATCA+TGG | + | chr3_1:2252711-2252730 | None:intergenic | 35.0% |
| GATCCTAAATTTGCAGAAGA+TGG | - | chr3_1:2252805-2252824 | Msa0343810:CDS | 35.0% | |
| AGCTTCTTGGATCAGATTTG+AGG | + | chr3_1:2252588-2252607 | None:intergenic | 40.0% | |
| AACAGGTCTAGCACTAATCA+TGG | - | chr3_1:2252621-2252640 | Msa0343810:CDS | 40.0% | |
| ! | GTTTAAGGCAGTGTTTGCTT+TGG | + | chr3_1:2252662-2252681 | None:intergenic | 40.0% |
| TAAGGAGAGCCATGATCAAA+AGG | - | chr3_1:2252699-2252718 | Msa0343810:CDS | 40.0% | |
| !! | GAAGCTTTGAATTCATGTGC+TGG | - | chr3_1:2252721-2252740 | Msa0343810:CDS | 40.0% |
| ! | TTGAGAGCTGCAACAGATTT+TGG | + | chr3_1:2252778-2252797 | None:intergenic | 40.0% |
| GCACCATCTTCTGCAAATTT+AGG | + | chr3_1:2252811-2252830 | None:intergenic | 40.0% | |
| ACTTGTGAGAATGGCTTCAA+TGG | - | chr3_1:2252856-2252875 | Msa0343810:CDS | 40.0% | |
| CTTGTGAGAATGGCTTCAAT+GGG | - | chr3_1:2252857-2252876 | Msa0343810:CDS | 40.0% | |
| GCAATATTCTCATCAGAGAG+TGG | + | chr3_1:2252889-2252908 | None:intergenic | 40.0% | |
| ACATCTGCATCAGAGCTTCT+TGG | + | chr3_1:2252601-2252620 | None:intergenic | 45.0% | |
| ! | AGCTCTGATGCAGATGTAAC+AGG | - | chr3_1:2252604-2252623 | Msa0343810:CDS | 45.0% |
| AGGTCTAGCACTAATCATGG+TGG | - | chr3_1:2252624-2252643 | Msa0343810:CDS | 45.0% | |
| GAAGCCACTACTTGTGAGAA+TGG | - | chr3_1:2252847-2252866 | Msa0343810:CDS | 45.0% | |
| GAAGCCATTCTCACAAGTAG+TGG | + | chr3_1:2252854-2252873 | None:intergenic | 45.0% | |
| TCTGTTGCAGCTCTCAAGCA+AGG | - | chr3_1:2252781-2252800 | Msa0343810:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 2252427 | 2252960 | 2252427 | ID=Msa0343810;Name=Msa0343810 |
| chr3_1 | mRNA | 2252427 | 2252960 | 2252427 | ID=Msa0343810-mRNA-1;Parent=Msa0343810;Name=Msa0343810-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|177 |
| chr3_1 | exon | 2252427 | 2252960 | 2252427 | ID=Msa0343810-mRNA-1:exon:702;Parent=Msa0343810-mRNA-1 |
| chr3_1 | CDS | 2252427 | 2252960 | 2252427 | ID=Msa0343810-mRNA-1:cds;Parent=Msa0343810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
