AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1404730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404730 MtrunA17_Chr3g0127431 96.936 359 11 0 2 360 3 361 0.0 719
Msa1404730 MtrunA17_Chr3g0127401 78.125 384 79 3 7 388 5 385 0.0 595
Msa1404730 MtrunA17_Chr8g0387641 56.574 251 107 2 65 314 503 752 6.42e-91 291
Msa1404730 MtrunA17_Chr1g0195741 54.264 258 117 1 61 317 916 1173 3.14e-90 295
Msa1404730 MtrunA17_Chr1g0195731 52.030 271 127 2 61 328 912 1182 9.57e-90 294
Msa1404730 MtrunA17_Chr4g0038101 48.084 287 148 1 65 350 703 989 1.41e-87 285
Msa1404730 MtrunA17_Chr3g0116131 54.065 246 112 1 61 305 880 1125 1.17e-85 282
Msa1404730 MtrunA17_Chr5g0397121 52.964 253 117 2 62 313 492 743 1.66e-84 274
Msa1404730 MtrunA17_Chr6g0462581 48.485 297 126 5 62 333 479 773 3.28e-82 267
Msa1404730 MtrunA17_Chr1g0202381 52.227 247 116 2 65 310 450 695 4.50e-77 253
Msa1404730 MtrunA17_Chr1g0202371 44.728 313 141 3 65 351 379 685 2.00e-76 250
Msa1404730 MtrunA17_Chr1g0204031 51.626 246 117 2 65 309 539 783 1.64e-72 242
Msa1404730 MtrunA17_Chr7g0246111 42.086 278 149 5 42 309 162 437 2.56e-61 205
Msa1404730 MtrunA17_Chr4g0052271 42.379 269 142 7 82 341 118 382 1.28e-57 193
Msa1404730 MtrunA17_Chr6g0459561 38.516 283 165 6 71 348 371 649 2.97e-56 195
Msa1404730 MtrunA17_Chr6g0459541 36.364 297 156 6 82 348 41 334 3.95e-53 180
Msa1404730 MtrunA17_Chr2g0321241 41.202 233 130 5 82 311 131 359 1.06e-50 176
Msa1404730 MtrunA17_Chr4g0004121 38.868 265 147 6 83 334 242 504 1.31e-50 177
Msa1404730 MtrunA17_Chr4g0026631 40.773 233 131 5 82 311 126 354 6.73e-49 171
Msa1404730 MtrunA17_Chr7g0264521 35.816 282 168 7 82 356 478 753 9.32e-48 173
Msa1404730 MtrunA17_Chr7g0264521 38.034 234 136 4 82 311 205 433 3.78e-45 166
Msa1404730 MtrunA17_Chr1g0180791 34.397 282 173 6 82 356 477 753 3.51e-46 169
Msa1404730 MtrunA17_Chr1g0180791 38.655 238 136 5 79 311 200 432 1.23e-45 167
Msa1404730 MtrunA17_Chr2g0327121 35.461 282 170 7 82 356 479 755 4.79e-46 168
Msa1404730 MtrunA17_Chr2g0327121 35.821 268 162 4 82 342 206 470 1.68e-43 161
Msa1404730 MtrunA17_Chr3g0145011 37.705 244 144 4 69 307 125 365 1.14e-45 161
Msa1404730 MtrunA17_Chr6g0459551 33.019 318 173 9 64 348 84 394 1.73e-45 162
Msa1404730 MtrunA17_Chr3g0141901 37.295 244 145 4 69 307 122 362 5.84e-45 159
Msa1404730 MtrunA17_Chr6g0459531 33.210 271 148 5 82 348 66 307 3.32e-42 150
Msa1404730 MtrunA17_Chr8g0345461 38.197 233 132 6 82 307 164 391 1.71e-41 151
Msa1404730 MtrunA17_Chr7g0255901 35.036 274 150 8 38 304 611 863 2.97e-40 152
Msa1404730 MtrunA17_Chr1g0192151 36.957 230 135 5 84 307 168 393 5.31e-39 144
Msa1404730 MtrunA17_Chr4g0014731 36.957 230 135 5 84 307 169 394 6.10e-39 144
Msa1404730 MtrunA17_Chr1g0200081 37.768 233 132 7 82 307 174 400 7.68e-39 144
Msa1404730 MtrunA17_Chr4g0050561 37.179 234 136 5 82 308 155 384 2.06e-38 142
Msa1404730 MtrunA17_Chr7g0255721 34.894 235 142 5 83 311 685 914 2.80e-37 143
Msa1404730 MtrunA17_Chr7g0255721 32.870 216 129 6 122 325 422 633 1.53e-27 115
Msa1404730 MtrunA17_Chr2g0277831 36.214 243 144 6 85 321 245 482 1.40e-36 141
Msa1404730 MtrunA17_Chr2g0277831 30.043 233 156 4 82 310 532 761 5.52e-32 127
Msa1404730 MtrunA17_Chr7g0217421 39.056 233 127 6 83 307 39 264 5.05e-36 135
Msa1404730 MtrunA17_Chr4g0052411 38.197 233 129 6 83 307 229 454 6.84e-36 139
Msa1404730 MtrunA17_Chr3g0108321 43.011 186 94 5 82 260 158 338 1.69e-35 134
Msa1404730 MtrunA17_Chr7g0255641 34.043 235 144 5 83 311 658 887 2.43e-35 138
Msa1404730 MtrunA17_Chr7g0255641 29.388 245 160 7 81 321 391 626 7.57e-25 107
Msa1404730 MtrunA17_Chr1g0182711 36.522 230 136 5 85 308 190 415 4.78e-35 134
Msa1404730 MtrunA17_Chr7g0260601 37.238 239 126 8 83 307 240 468 9.15e-35 135
Msa1404730 MtrunA17_Chr2g0297281 37.339 233 131 6 83 307 286 511 1.73e-34 135
Msa1404730 MtrunA17_Chr6g0456351 37.179 234 139 4 78 307 312 541 1.91e-34 134
Msa1404730 MtrunA17_Chr4g0033451 35.341 249 144 7 72 308 367 610 2.06e-34 135
Msa1404730 MtrunA17_Chr7g0262801 36.087 230 137 5 85 308 189 414 2.20e-34 132
Msa1404730 MtrunA17_Chr3g0143721 37.344 241 136 5 83 315 249 482 1.21e-33 132
Msa1404730 MtrunA17_Chr4g0031821 36.250 240 127 8 83 307 320 548 1.94e-33 132
Msa1404730 MtrunA17_Chr2g0289231 32.234 273 166 7 82 344 296 559 5.31e-33 130
Msa1404730 MtrunA17_Chr3g0129971 39.095 243 127 9 83 311 296 531 3.96e-32 128
Msa1404730 MtrunA17_Chr7g0248361 37.500 240 124 9 83 307 192 420 6.65e-32 127
Msa1404730 MtrunA17_Chr4g0043381 31.186 295 185 6 85 367 839 1127 1.35e-31 127
Msa1404730 MtrunA17_Chr5g0432631 35.531 273 149 8 82 345 228 482 1.43e-31 126
Msa1404730 MtrunA17_Chr6g0476861 35.833 240 128 8 83 307 197 425 2.29e-31 125
Msa1404730 MtrunA17_Chr3g0135761 33.439 314 160 10 46 345 197 475 3.05e-31 125
Msa1404730 MtrunA17_Chr3g0135721 34.559 272 151 8 83 345 224 477 6.20e-31 124
Msa1404730 MtrunA17_Chr8g0362771 36.555 238 137 7 82 314 236 464 7.34e-31 124
Msa1404730 MtrunA17_Chr7g0255691 36.726 226 127 8 86 301 20 239 1.06e-30 123
Msa1404730 MtrunA17_Chr7g0255691 35.169 236 141 5 83 311 286 516 1.11e-29 120
Msa1404730 MtrunA17_Chr2g0327231 41.212 165 91 3 82 243 345 506 1.53e-30 122
Msa1404730 MtrunA17_Chr2g0331161 31.405 242 139 7 81 308 413 641 4.64e-30 122
Msa1404730 MtrunA17_Chr1g0158881 38.288 222 126 6 85 302 331 545 5.73e-30 122
Msa1404730 MtrunA17_Chr7g0255651 33.473 239 151 4 83 316 282 517 1.56e-29 120
Msa1404730 MtrunA17_Chr7g0255651 35.233 193 116 5 122 307 51 241 2.09e-24 105
Msa1404730 MtrunA17_Chr5g0429571 36.820 239 129 8 83 308 313 542 1.61e-29 120
Msa1404730 MtrunA17_Chr2g0316851 30.682 264 161 8 85 331 596 854 1.94e-29 120
Msa1404730 MtrunA17_Chr3g0094871 33.898 236 137 7 83 307 11 238 2.27e-29 118
Msa1404730 MtrunA17_Chr3g0104211 36.667 240 128 7 83 308 304 533 4.36e-29 119
Msa1404730 MtrunA17_Chr3g0086411 28.013 307 181 9 39 310 158 459 1.72e-28 116
Msa1404730 MtrunA17_Chr3g0086411 30.818 159 100 4 172 321 7 164 1.40e-14 75.1
Msa1404730 MtrunA17_Chr8g0388371 28.750 320 185 9 83 387 404 695 1.86e-27 114
Msa1404730 MtrunA17_Chr3g0086401 31.818 242 140 5 82 301 384 622 7.07e-27 112
Msa1404730 MtrunA17_Chr3g0086401 31.416 226 137 6 102 312 105 327 3.37e-23 101
Msa1404730 MtrunA17_Chr5g0432641 35.065 231 129 5 122 345 228 444 3.21e-26 110
Msa1404730 MtrunA17_Chr3g0135781 40.361 166 89 6 82 244 54 212 7.62e-24 98.2
Msa1404730 MtrunA17_Chr8g0357301 31.980 197 119 6 122 307 507 699 3.92e-22 98.6
Msa1404730 MtrunA17_Chr4g0047411 29.661 236 136 9 122 334 214 442 1.77e-17 84.3
Msa1404730 MtrunA17_Chr5g0404291 36.806 144 84 5 119 260 386 524 7.00e-17 82.4
Msa1404730 MtrunA17_Chr1g0190401 30.890 191 116 7 83 264 677 860 2.32e-16 81.3
Msa1404730 MtrunA17_Chr6g0450161 34.868 152 92 5 119 268 361 507 9.35e-16 79.0
Msa1404730 MtrunA17_Chr1g0168321 33.951 162 95 5 155 307 197 355 1.08e-15 78.6
Msa1404730 MtrunA17_Chr4g0039781 32.903 155 91 5 119 268 367 513 3.93e-15 77.0
Msa1404730 MtrunA17_Chr5g0432391 33.099 142 91 2 163 302 82 221 8.99e-14 72.4
Msa1404730 MtrunA17_Chr4g0062081 53.731 67 26 2 88 152 12 75 1.64e-13 66.2
Msa1404730 MtrunA17_Chr3g0116941 29.310 174 97 5 117 269 192 360 1.91e-12 68.6
Msa1404730 MtrunA17_Chr1g0204051 36.842 76 47 1 212 287 1 75 2.93e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404730 AT4G27680.1 75.521 384 93 1 7 389 5 388 0.0 599
Msa1404730 AT5G53540.1 71.979 389 107 2 3 389 4 392 0.0 568
Msa1404730 AT4G24860.4 53.309 272 121 2 61 326 768 1039 4.40e-90 293
Msa1404730 AT4G24860.1 53.309 272 121 2 61 326 795 1066 4.92e-90 294
Msa1404730 AT4G24860.3 53.309 272 121 2 61 326 793 1064 6.88e-90 293
Msa1404730 AT4G24860.2 53.309 272 121 2 61 326 791 1062 7.77e-90 293
Msa1404730 AT1G64110.1 53.992 263 115 3 65 322 495 756 1.82e-89 287
Msa1404730 AT1G64110.2 53.992 263 115 3 65 322 500 761 1.89e-89 287
Msa1404730 AT1G64110.4 53.992 263 115 3 65 322 500 761 1.89e-89 287
Msa1404730 AT1G64110.5 53.992 263 115 3 65 322 498 759 2.02e-89 287
Msa1404730 AT1G64110.3 53.992 263 115 3 65 322 498 759 2.02e-89 287
Msa1404730 AT1G02890.1 45.808 334 151 4 15 333 888 1206 3.76e-89 293
Msa1404730 AT4G02480.1 54.980 251 112 1 61 310 938 1188 1.21e-88 291
Msa1404730 AT1G50140.2 47.931 290 150 1 62 350 668 957 1.35e-87 285
Msa1404730 AT1G50140.5 47.931 290 150 1 62 350 668 957 1.35e-87 285
Msa1404730 AT1G50140.3 47.931 290 150 1 62 350 690 979 1.59e-87 285
Msa1404730 AT1G50140.1 47.931 290 150 1 62 350 690 979 1.59e-87 285
Msa1404730 AT1G50140.4 47.931 290 150 1 62 350 690 979 1.59e-87 285
Msa1404730 AT3G19740.1 50.549 273 131 2 62 330 680 952 8.74e-85 278
Msa1404730 AT5G52882.1 51.894 264 124 3 61 323 492 753 5.27e-84 273
Msa1404730 AT4G28000.3 48.951 286 141 4 65 349 499 780 5.45e-83 270
Msa1404730 AT4G28000.1 48.951 286 141 4 65 349 497 778 7.97e-83 270
Msa1404730 AT4G28000.2 48.951 286 141 4 65 349 499 780 8.23e-83 270
Msa1404730 AT1G02890.2 41.018 334 139 5 15 333 888 1178 3.67e-70 239
Msa1404730 AT1G62130.2 46.269 268 125 4 47 314 715 963 1.56e-69 236
Msa1404730 AT1G62130.1 41.071 336 153 7 47 356 722 1038 1.73e-69 236
Msa1404730 AT2G45500.4 44.622 251 129 5 65 309 59 305 3.86e-65 211
Msa1404730 AT2G45500.3 44.622 251 129 5 65 309 59 305 3.86e-65 211
Msa1404730 AT2G45500.2 44.622 251 129 5 65 309 192 438 1.14e-63 211
Msa1404730 AT2G45500.1 44.622 251 129 5 65 309 196 442 1.16e-63 211
Msa1404730 AT2G45500.5 44.622 251 129 5 65 309 202 448 2.55e-63 211
Msa1404730 AT2G34560.1 40.426 282 150 7 69 341 92 364 1.16e-58 196
Msa1404730 AT2G34560.2 40.426 282 150 7 69 341 101 373 1.60e-58 195
Msa1404730 AT3G27120.1 39.161 286 159 7 71 348 389 667 1.78e-55 194
Msa1404730 AT1G80350.1 38.521 257 145 5 83 332 236 486 1.18e-50 178
Msa1404730 AT2G27600.1 40.343 233 132 5 82 311 128 356 3.72e-48 169
Msa1404730 AT5G03340.1 35.461 282 170 6 82 356 476 752 1.77e-47 173
Msa1404730 AT5G03340.1 38.034 234 136 4 82 311 203 431 7.37e-45 166
Msa1404730 AT1G45000.1 38.115 244 143 4 69 307 122 362 3.65e-46 163
Msa1404730 AT3G09840.1 38.462 234 135 4 82 311 203 431 1.72e-45 167
Msa1404730 AT3G09840.1 34.276 283 172 6 82 356 476 752 1.90e-45 167
Msa1404730 AT3G53230.1 36.694 248 147 5 82 322 477 721 2.74e-45 167
Msa1404730 AT3G53230.1 35.556 270 160 5 82 342 204 468 8.45e-45 165
Msa1404730 AT5G43010.1 37.295 244 145 4 69 307 122 362 4.46e-45 160
Msa1404730 AT1G53750.1 38.197 233 132 6 82 307 164 391 3.42e-41 150
Msa1404730 AT5G58290.1 35.249 261 152 6 82 330 151 406 7.01e-40 146
Msa1404730 AT1G03000.1 36.207 232 139 6 78 303 650 878 3.44e-39 149
Msa1404730 AT3G05530.1 36.797 231 134 5 84 307 169 394 4.51e-39 145
Msa1404730 AT5G19990.1 37.179 234 136 5 82 308 157 386 1.65e-38 143
Msa1404730 AT5G19990.3 37.179 234 136 5 82 308 157 386 1.65e-38 143
Msa1404730 AT5G19990.2 37.179 234 136 5 82 308 99 328 1.84e-38 142
Msa1404730 AT5G20000.1 37.179 234 136 5 82 308 157 386 2.15e-38 143
Msa1404730 AT1G53780.2 37.339 233 132 6 82 306 357 583 5.22e-38 145
Msa1404730 AT1G53780.3 37.339 233 132 6 82 306 335 561 5.74e-38 144
Msa1404730 AT1G53780.1 37.339 233 132 6 82 306 336 562 5.90e-38 144
Msa1404730 AT1G53780.4 37.339 233 132 6 82 306 336 562 5.90e-38 144
Msa1404730 AT1G09100.1 36.797 231 134 5 84 307 168 393 7.31e-38 141
Msa1404730 AT3G56690.1 35.294 238 143 5 83 314 721 953 1.56e-37 144
Msa1404730 AT3G56690.1 32.364 275 170 6 41 311 347 609 5.02e-37 143
Msa1404730 AT2G30950.1 38.197 233 129 6 83 307 224 449 1.99e-35 138
Msa1404730 AT2G30950.2 38.197 233 129 6 83 307 224 449 1.99e-35 138
Msa1404730 AT2G30950.3 38.197 233 129 6 83 307 224 449 1.99e-35 138
Msa1404730 AT2G30950.4 38.197 233 129 6 83 307 228 453 2.20e-35 138
Msa1404730 AT1G06430.1 38.627 233 128 6 83 307 217 442 2.40e-35 137
Msa1404730 AT1G06430.3 38.627 233 128 6 83 307 217 442 2.40e-35 137
Msa1404730 AT1G06430.2 38.627 233 128 6 83 307 217 442 2.40e-35 137
Msa1404730 AT5G15250.1 38.136 236 125 8 83 307 221 446 4.90e-35 137
Msa1404730 AT1G05910.1 35.341 249 144 7 72 308 365 608 6.15e-35 137
Msa1404730 AT4G29040.1 36.797 231 134 6 85 308 187 412 1.66e-34 132
Msa1404730 AT2G03670.2 37.104 221 130 5 88 301 24 242 1.68e-34 132
Msa1404730 AT2G03670.2 38.596 114 69 1 83 196 283 395 5.18e-14 73.6
Msa1404730 AT3G01610.2 35.124 242 142 5 85 314 232 470 1.75e-34 135
Msa1404730 AT3G01610.2 33.476 233 146 5 82 310 524 751 6.56e-31 125
Msa1404730 AT2G20140.1 36.797 231 134 6 85 308 187 412 2.08e-34 132
Msa1404730 AT3G01610.1 35.124 242 142 5 85 314 232 470 2.11e-34 135
Msa1404730 AT3G01610.1 33.476 233 146 5 82 310 524 751 7.49e-31 125
Msa1404730 AT3G02450.1 35.766 274 143 10 83 335 331 592 3.64e-34 134
Msa1404730 AT5G42270.1 36.929 241 137 5 83 315 247 480 8.76e-34 133
Msa1404730 AT2G03670.1 37.104 221 130 5 88 301 24 242 9.98e-34 132
Msa1404730 AT2G03670.1 34.483 232 143 5 83 308 283 511 2.54e-29 120
Msa1404730 AT1G50250.1 36.929 241 137 5 83 315 259 492 1.42e-33 132
Msa1404730 AT5G08470.1 34.583 240 152 3 85 322 843 1079 4.16e-32 129
Msa1404730 AT5G08470.3 34.583 240 152 3 85 322 834 1070 4.23e-32 129
Msa1404730 AT1G07510.1 35.685 241 129 8 83 308 324 553 7.28e-32 128
Msa1404730 AT2G29080.1 35.983 239 130 7 83 308 319 547 9.34e-32 127
Msa1404730 AT2G26140.1 37.500 224 130 5 83 302 224 441 5.40e-31 125
Msa1404730 AT5G53170.1 37.662 231 133 6 76 302 352 575 7.75e-31 125
Msa1404730 AT5G15250.2 34.601 263 128 9 83 311 221 473 9.16e-31 123
Msa1404730 AT3G47060.1 36.402 239 130 8 83 308 322 551 1.28e-30 124
Msa1404730 AT5G64580.1 37.037 243 132 9 83 311 313 548 3.50e-30 123
Msa1404730 AT5G58870.1 36.402 239 130 8 83 308 326 555 8.58e-30 122
Msa1404730 AT4G04180.2 31.349 252 152 6 82 321 310 552 1.74e-29 120
Msa1404730 AT4G04180.1 31.349 252 152 6 82 321 315 557 2.11e-29 120
Msa1404730 AT3G16290.1 30.605 281 161 9 81 342 405 670 1.14e-28 118
Msa1404730 AT4G23940.1 33.471 242 138 6 83 308 427 661 3.52e-28 117
Msa1404730 AT3G15120.2 29.502 261 167 6 85 331 728 985 3.75e-28 117
Msa1404730 AT3G15120.1 29.502 261 167 6 85 331 718 975 3.81e-28 117
Msa1404730 AT1G79560.3 35.354 198 113 6 122 308 527 720 3.16e-26 111
Msa1404730 AT1G79560.1 35.354 198 113 6 122 308 527 720 3.28e-26 111
Msa1404730 AT1G79560.2 35.354 198 113 6 122 308 527 720 3.60e-26 111
Msa1404730 AT1G45000.2 37.908 153 91 2 69 218 122 273 9.41e-25 103
Msa1404730 AT5G08470.2 35.000 160 100 3 85 244 843 998 2.24e-17 84.7
Msa1404730 AT4G04910.1 30.275 218 121 10 122 315 251 461 2.74e-16 81.3
Msa1404730 AT3G04340.1 31.156 199 118 8 75 264 776 964 4.74e-16 80.9
Msa1404730 AT2G18330.1 34.722 144 87 5 119 260 383 521 1.56e-15 79.0
Msa1404730 AT3G03060.1 32.895 152 95 5 120 269 385 531 8.42e-15 76.6
Msa1404730 AT5G16930.1 32.895 152 95 5 120 269 397 543 8.71e-15 76.6
Msa1404730 AT4G36580.1 34.028 144 88 5 119 260 380 518 2.38e-14 75.1

Find 94 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCTCAACCCTCTCTTTC+TGG 0.149964 tig0023079:+76466 None:intergenic
ACAAACTTCGATTCTGATTC+TGG 0.222136 tig0023079:+78510 None:intergenic
CAATGAATTCTCTAAGCTTA+TGG 0.225338 tig0023079:-75478 Msa1404730:CDS
GATATTGAAGGTTATCTTAA+AGG 0.238298 tig0023079:-76448 Msa1404730:CDS
TGGTGAGTCAGTTGATTATC+AGG 0.256999 tig0023079:-75514 Msa1404730:CDS
TATTTCTTCTGATTACTGTT+GGG 0.304748 tig0023079:-75186 Msa1404730:three_prime_UTR
GTTTGCCCATGGGAAACTAT+TGG 0.318144 tig0023079:-78190 Msa1404730:CDS
AAACTGAGTTCATGGCTCTA+TGG 0.320401 tig0023079:-76728 Msa1404730:CDS
CTTATAAGCCAAGCTAAATA+TGG 0.332003 tig0023079:+76839 None:intergenic
TCATCCAGCAGTTCTCTAAT+AGG 0.339066 tig0023079:+76321 None:intergenic
TTTGCCCATGGGAAACTATT+GGG 0.350438 tig0023079:-78189 Msa1404730:CDS
GAGCGGCTTTCATTAATGTT+AGG 0.358887 tig0023079:-78086 Msa1404730:CDS
AAATATGAAAACTGAGTTCA+TGG 0.370471 tig0023079:-76736 Msa1404730:CDS
ATTCAAATGCTTAAGAATCC+TGG 0.378637 tig0023079:+75424 None:intergenic
GAAATAAAGTATGGCATGTA+TGG 0.379242 tig0023079:+75203 None:intergenic
TTATTTCTTCTGATTACTGT+TGG 0.386748 tig0023079:-75187 Msa1404730:three_prime_UTR
TCAACCCTCTCTTTCTGGTC+TGG 0.390685 tig0023079:+76471 None:intergenic
TACTTTCGTTTAGCTTAGTC+CGG 0.399875 tig0023079:-75383 Msa1404730:three_prime_UTR
ATATTGAAGGTTATCTTAAA+GGG 0.410430 tig0023079:-76447 Msa1404730:CDS
GAAACTATTGGGTCCTCAAA+AGG 0.416833 tig0023079:-78178 Msa1404730:CDS
TAATCAGAAGAAATAAAGTA+TGG 0.419837 tig0023079:+75194 None:intergenic
CAAAAGGGTGTCTTGTTGTA+TGG 0.420182 tig0023079:-78162 Msa1404730:CDS
GATGTTGAGTTTGACAGTAT+TGG 0.422610 tig0023079:-78279 Msa1404730:CDS
AAGAATCCTGGGAATCTTGT+TGG 0.427563 tig0023079:+75436 None:intergenic
CGGCTATTACATCTTCGTAT+TGG 0.441145 tig0023079:+78328 None:intergenic
ATTTCTTCTGATTACTGTTG+GGG 0.443077 tig0023079:-75185 Msa1404730:three_prime_UTR
ATAGACTTCAATTATATAGC+TGG 0.444468 tig0023079:-76405 Msa1404730:CDS
GTTTGACAGTATTGGAGGAT+TGG 0.455697 tig0023079:-78271 Msa1404730:CDS
TTTCCTCAAGCCTTTGAAGT+TGG 0.462777 tig0023079:-76498 Msa1404730:CDS
CGGGTCAAGATATGAATAAC+CGG 0.463608 tig0023079:+78431 None:intergenic
AGTGTCTGCCATATTTAGCT+TGG 0.464625 tig0023079:-76847 Msa1404730:CDS
AACTCAACATCGATGTGGTC+AGG 0.468203 tig0023079:+78291 None:intergenic
AGAATGCCCTTTCCACTTCA+CGG 0.470894 tig0023079:-75590 Msa1404730:CDS
AGCAATAGCTTTAGCAAGCA+TGG 0.476844 tig0023079:+78119 None:intergenic
TCTATTTGATCTTTGCAAGA+AGG 0.478539 tig0023079:-76355 Msa1404730:CDS
TTGTATGGACCACCTGGTAC+TGG 0.480480 tig0023079:-78147 Msa1404730:CDS
AGAGAGGGTTGAGATATTGA+AGG 0.482658 tig0023079:-76460 Msa1404730:CDS
AACAATTCTGATCGTTTCAA+AGG 0.485349 tig0023079:+78210 None:intergenic
TCAAACTGCGACAAAGGTCT+AGG 0.485635 tig0023079:+75618 None:intergenic
TGCTGGATGACGAGAAGAAA+GGG 0.487745 tig0023079:-76308 Msa1404730:CDS
TGTAGAGAATGCTCGAGTTA+TGG 0.489871 tig0023079:-76574 Msa1404730:intron
TTCAAATGCTTAAGAATCCT+GGG 0.495703 tig0023079:+75425 None:intergenic
GTTGAGTTTGACAGTATTGG+AGG 0.500647 tig0023079:-78276 Msa1404730:CDS
CGATCAGAATTGTTTGCCCA+TGG 0.502032 tig0023079:-78201 Msa1404730:CDS
AAGTAATCGCTGTTGCAATC+CGG 0.509702 tig0023079:+75364 None:intergenic
CGTCGTCCGTGAAGTGGAAA+GGG 0.510369 tig0023079:+75584 None:intergenic
TCTAAATCAAACTGCGACAA+AGG 0.511155 tig0023079:+75612 None:intergenic
ACGGCTGTTGCAAGCGAGTA+CGG 0.511325 tig0023079:-75564 Msa1404730:CDS
AACTGAGTTCATGGCTCTAT+GGG 0.515199 tig0023079:-76727 Msa1404730:CDS
AGAAAACGAATCTCCATGCT+TGG 0.518329 tig0023079:+78607 None:intergenic
AAACTATTGGGTCCTCAAAA+GGG 0.519588 tig0023079:-78177 Msa1404730:CDS
CCGTCGTCCGTGAAGTGGAA+AGG 0.521667 tig0023079:+75583 None:intergenic
TATATAGCTGGTTTATGCAA+GGG 0.522653 tig0023079:-76393 Msa1404730:CDS
AATCTCATGAGCATGTGGTT+CGG 0.528373 tig0023079:-78057 Msa1404730:CDS
AAACGAATCTCCATGCTTGG+AGG 0.528719 tig0023079:+78610 None:intergenic
GTCTGGAATTCCAACTTCAA+AGG 0.530935 tig0023079:+76488 None:intergenic
GAGTTCATGGCTCTATGGGA+TGG 0.533268 tig0023079:-76723 Msa1404730:CDS
AGTTCATGGCTCTATGGGAT+GGG 0.534957 tig0023079:-76722 Msa1404730:CDS
TAACCTCCAACAAGATTCCC+AGG 0.537269 tig0023079:-75442 Msa1404730:CDS
CGAATCTCCATGCTTGGAGG+AGG 0.540378 tig0023079:+78613 None:intergenic
GTCTTGTTGTATGGACCACC+TGG 0.542959 tig0023079:-78153 Msa1404730:CDS
TTCGGCGATGCAACTAAGCT+TGG 0.543662 tig0023079:-78039 Msa1404730:intron
TTATATAGCTGGTTTATGCA+AGG 0.548540 tig0023079:-76394 Msa1404730:CDS
GATCAGAATTGTTTGCCCAT+GGG 0.551579 tig0023079:-78200 Msa1404730:CDS
GAGGACCCAATAGTTTCCCA+TGG 0.551802 tig0023079:+78184 None:intergenic
CTGCTGGATGACGAGAAGAA+AGG 0.553382 tig0023079:-76309 Msa1404730:CDS
AAAGCAATTGCAAATCGTCT+TGG 0.556831 tig0023079:-78372 Msa1404730:CDS
TCATCAATGAATATGATAGC+AGG 0.558085 tig0023079:+76810 None:intergenic
AATCCTGGGAATCTTGTTGG+AGG 0.558745 tig0023079:+75439 None:intergenic
AACAGCCGTCGTCCGTGAAG+TGG 0.561036 tig0023079:+75578 None:intergenic
ATTGCTTCATCAAGTTCTGA+AGG 0.564553 tig0023079:+76531 None:intergenic
ACTAACTCCTCCTCCAAGCA+TGG 0.566597 tig0023079:-78620 Msa1404730:five_prime_UTR
GTCAGGATTTATAACATCAC+CGG 0.568581 tig0023079:+78308 None:intergenic
GGTTTATGCAAGGGTTACAC+AGG 0.570100 tig0023079:-76384 Msa1404730:CDS
AGGACCCAATAGTTTCCCAT+GGG 0.585926 tig0023079:+78185 None:intergenic
ATTCCAACTTCAAAGGCTTG+AGG 0.586616 tig0023079:+76495 None:intergenic
GGTTATCTTAAAGGGCGAGA+GGG 0.590443 tig0023079:-76439 Msa1404730:CDS
CAATACGAAGATGTAATAGC+CGG 0.594799 tig0023079:-78327 Msa1404730:CDS
GGTGAGTCAGTTGATTATCA+GGG 0.595184 tig0023079:-75513 Msa1404730:CDS
TTCTTCTCGGTAGAAGAGAA+CGG 0.596831 tig0023079:-78588 Msa1404730:five_prime_UTR
AAAGCTATTGCTAAGGAGTG+TGG 0.598472 tig0023079:-78108 Msa1404730:CDS
GCTTGCTAAAGCTATTGCTA+AGG 0.615516 tig0023079:-78115 Msa1404730:CDS
GAATTCCAGACCAGAAAGAG+AGG 0.625831 tig0023079:-76476 Msa1404730:CDS
TTGCTTCATCAAGTTCTGAA+GGG 0.630399 tig0023079:+76532 None:intergenic
CCTTTCCACTTCACGGACGA+CGG 0.635737 tig0023079:-75583 Msa1404730:CDS
AACTTGATGAAGCAATACTT+CGG 0.636206 tig0023079:-76524 Msa1404730:CDS
AGGTTATCTTAAAGGGCGAG+AGG 0.640020 tig0023079:-76440 Msa1404730:CDS
TGTCTAATCTCATGAGCATG+TGG 0.640666 tig0023079:-78062 Msa1404730:CDS
TATTGCTAAGGAGTGTGGAG+CGG 0.650592 tig0023079:-78103 Msa1404730:CDS
AGTTGCTTAATATTGTACAC+CGG 0.650727 tig0023079:-78450 Msa1404730:CDS
AATTCCAGACCAGAAAGAGA+GGG 0.661161 tig0023079:-76475 Msa1404730:CDS
GGTAGAAGAGAACGGTTCTG+CGG 0.682650 tig0023079:-78580 Msa1404730:five_prime_UTR
TGTCAAACTCAACATCGATG+TGG 0.699965 tig0023079:+78286 None:intergenic
ACGTCGTTCATCAGAACACG+AGG 0.705850 tig0023079:-76766 Msa1404730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTAAATATTTTTTCTTT+TGG - tig0023079:77145-77164 Msa1404730:intron 10.0%
!! AACATAAAAATAAAATAACA+CGG + tig0023079:78103-78122 None:intergenic 10.0%
!!! TTATTTTATTTTTATGTTTC+AGG - tig0023079:78105-78124 Msa1404730:CDS 10.0%
!! GATTAGATACAATTATATTA+GGG + tig0023079:76810-76829 None:intergenic 15.0%
!! AGATTAGATACAATTATATT+AGG + tig0023079:76811-76830 None:intergenic 15.0%
!! AATAACATAATATTAACGAT+AGG + tig0023079:76874-76893 None:intergenic 15.0%
!! TAAAAAAAAACTGGTAAAAT+TGG - tig0023079:77819-77838 Msa1404730:intron 15.0%
!!! AACTGATTTGATTTTATTAG+CGG - tig0023079:75261-75280 Msa1404730:intron 20.0%
!! AGTTATATTACATTTACAAG+AGG + tig0023079:76140-76159 None:intergenic 20.0%
!! TTCATTAGAATATTTAACGA+GGG - tig0023079:76748-76767 Msa1404730:CDS 20.0%
!!! TATTTTGAAAATTTATGCCA+TGG + tig0023079:77099-77118 None:intergenic 20.0%
!!! GCATAAATTTTCAAAATATG+AGG - tig0023079:77101-77120 Msa1404730:intron 20.0%
!! ATATTGAAGGTTATCTTAAA+GGG - tig0023079:77301-77320 Msa1404730:intron 20.0%
!! AAAAATGATAATATCCACTA+TGG + tig0023079:77789-77808 None:intergenic 20.0%
!!! ATTTCACTTTTAAACAATTC+GGG + tig0023079:77888-77907 None:intergenic 20.0%
!!! GATTTCACTTTTAAACAATT+CGG + tig0023079:77889-77908 None:intergenic 20.0%
!! TAATCAGAAGAAATAAAGTA+TGG + tig0023079:78557-78576 None:intergenic 20.0%
!!! TATTGGTTTGTTTTAATGAG+GGG + tig0023079:75406-75425 None:intergenic 25.0%
!!! GTATTGGTTTGTTTTAATGA+GGG + tig0023079:75407-75426 None:intergenic 25.0%
!! TTTTTGCTTTGATGTATCTA+TGG - tig0023079:75928-75947 Msa1404730:intron 25.0%
! ATAGCAGAAAAGGTTAATAT+TGG + tig0023079:75960-75979 None:intergenic 25.0%
! TTCATTCTGTTTGATAGATA+AGG - tig0023079:76340-76359 Msa1404730:CDS 25.0%
!! AATGATAGTGACCTTTATAT+TGG - tig0023079:76399-76418 Msa1404730:CDS 25.0%
!! ATGATAGTGACCTTTATATT+GGG - tig0023079:76400-76419 Msa1404730:CDS 25.0%
!! AAACTTGTTTCCCAATATAA+AGG + tig0023079:76413-76432 None:intergenic 25.0%
!! ATTATAAACAACTCCAGTTT+TGG - tig0023079:76536-76555 Msa1404730:CDS 25.0%
!!! CAGTTTTGGAATTAGTAAAA+GGG - tig0023079:76550-76569 Msa1404730:CDS 25.0%
! AATAATTAGAGAACCAACAA+GGG + tig0023079:76658-76677 None:intergenic 25.0%
! CTTCATTAGAATATTTAACG+AGG - tig0023079:76747-76766 Msa1404730:CDS 25.0%
!! AATGTTTTTCATTTTGAACG+TGG - tig0023079:76842-76861 Msa1404730:CDS 25.0%
! AAATATGAAAACTGAGTTCA+TGG - tig0023079:77012-77031 Msa1404730:intron 25.0%
! GATATTGAAGGTTATCTTAA+AGG - tig0023079:77300-77319 Msa1404730:intron 25.0%
! ATAGACTTCAATTATATAGC+TGG - tig0023079:77343-77362 Msa1404730:intron 25.0%
!! TTATTTCTTCTGATTACTGT+TGG - tig0023079:78561-78580 Msa1404730:five_prime_UTR 25.0%
! TATTTCTTCTGATTACTGTT+GGG - tig0023079:78562-78581 Msa1404730:five_prime_UTR 25.0%
AGTTGCTTAATATTGTACAC+CGG - tig0023079:75298-75317 Msa1404730:intron 30.0%
!!! CGTATTGGTTTGTTTTAATG+AGG + tig0023079:75408-75427 None:intergenic 30.0%
AACAATTCTGATCGTTTCAA+AGG + tig0023079:75541-75560 None:intergenic 30.0%
TATTAATGATGCACATTCAC+TGG + tig0023079:75821-75840 None:intergenic 30.0%
! TAGTCCATTTGCAAGATTTT+GGG + tig0023079:75857-75876 None:intergenic 30.0%
! TTAGTCCATTTGCAAGATTT+TGG + tig0023079:75858-75877 None:intergenic 30.0%
!! AAATCTTGCAAATGGACTAA+AGG - tig0023079:75858-75877 Msa1404730:intron 30.0%
! TAAAGGACTCACAATGATTT+TGG - tig0023079:75875-75894 Msa1404730:intron 30.0%
! AACCTTTTCTGCTATAAAAC+CGG - tig0023079:75965-75984 Msa1404730:intron 30.0%
AGCAACCTCAATGATAAAAA+AGG + tig0023079:76242-76261 None:intergenic 30.0%
ATTGCCATTAGATAAACAGA+TGG + tig0023079:76366-76385 None:intergenic 30.0%
! CCTTTTACTAATTCCAAAAC+TGG + tig0023079:76552-76571 None:intergenic 30.0%
!!! CCAGTTTTGGAATTAGTAAA+AGG - tig0023079:76549-76568 Msa1404730:CDS 30.0%
CAATAATTAGAGAACCAACA+AGG + tig0023079:76659-76678 None:intergenic 30.0%
! TTGTTGATTAGCAAAATGTG+TGG - tig0023079:76676-76695 Msa1404730:intron 30.0%
TAGCAAAATGTGTGGTTATT+TGG - tig0023079:76684-76703 Msa1404730:intron 30.0%
! TTTCATTTTGAACGTGGTTT+TGG - tig0023079:76848-76867 Msa1404730:CDS 30.0%
CTTATAAGCCAAGCTAAATA+TGG + tig0023079:76912-76931 None:intergenic 30.0%
TCATCAATGAATATGATAGC+AGG + tig0023079:76941-76960 None:intergenic 30.0%
!!! TGATGAAGTAGACAGTTTTT+TGG - tig0023079:76955-76974 Msa1404730:intron 30.0%
!!! GATGAAGTAGACAGTTTTTT+GGG - tig0023079:76956-76975 Msa1404730:intron 30.0%
!! GTCAACTACTTTAGAACTTA+CGG + tig0023079:77063-77082 None:intergenic 30.0%
! TGACACTTAATTTTCATCCA+TGG - tig0023079:77079-77098 Msa1404730:intron 30.0%
AACTTGATGAAGCAATACTT+CGG - tig0023079:77224-77243 Msa1404730:intron 30.0%
! TTATATAGCTGGTTTATGCA+AGG - tig0023079:77354-77373 Msa1404730:intron 30.0%
! TATATAGCTGGTTTATGCAA+GGG - tig0023079:77355-77374 Msa1404730:intron 30.0%
TCTATTTGATCTTTGCAAGA+AGG - tig0023079:77393-77412 Msa1404730:intron 30.0%
TGTCTAGAAGAGAAAACTAA+AGG - tig0023079:77610-77629 Msa1404730:intron 30.0%
! TCGCACAAATCTAAAACTTA+GGG + tig0023079:77655-77674 None:intergenic 30.0%
! ATCGCACAAATCTAAAACTT+AGG + tig0023079:77656-77675 None:intergenic 30.0%
!! GTTTTAGATTTGTGCGATTT+TGG - tig0023079:77658-77677 Msa1404730:intron 30.0%
AATTAGGGAAAAACATGTGA+GGG + tig0023079:77718-77737 None:intergenic 30.0%
AAATTAGGGAAAAACATGTG+AGG + tig0023079:77719-77738 None:intergenic 30.0%
!! TACGGCAAATTTTAATGACA+TGG - tig0023079:77747-77766 Msa1404730:intron 30.0%
!!! TAATTTTGAAGTGAAGGGAA+AGG + tig0023079:77927-77946 None:intergenic 30.0%
TTTCCCTTCACTTCAAAATT+AGG - tig0023079:77926-77945 Msa1404730:intron 30.0%
TTCCCTTCACTTCAAAATTA+GGG - tig0023079:77927-77946 Msa1404730:intron 30.0%
!!! TTCCCTAATTTTGAAGTGAA+GGG + tig0023079:77932-77951 None:intergenic 30.0%
!!! TTTCCCTAATTTTGAAGTGA+AGG + tig0023079:77933-77952 None:intergenic 30.0%
CAATGAATTCTCTAAGCTTA+TGG - tig0023079:78270-78289 Msa1404730:CDS 30.0%
TTCAAATGCTTAAGAATCCT+GGG + tig0023079:78326-78345 None:intergenic 30.0%
ATTCAAATGCTTAAGAATCC+TGG + tig0023079:78327-78346 None:intergenic 30.0%
GAAATAAAGTATGGCATGTA+TGG + tig0023079:78548-78567 None:intergenic 30.0%
ATTTCTTCTGATTACTGTTG+GGG - tig0023079:78563-78582 Msa1404730:five_prime_UTR 30.0%
! ATGGAGATTCGTTTTCTTCT+CGG - tig0023079:75147-75166 Msa1404730:three_prime_UTR 35.0%
ACAAACTTCGATTCTGATTC+TGG + tig0023079:75241-75260 None:intergenic 35.0%
! TTTTTGGATGATTGACGGTT+CGG + tig0023079:75340-75359 None:intergenic 35.0%
!!! GTGCTTTTTTGGATGATTGA+CGG + tig0023079:75345-75364 None:intergenic 35.0%
!!! CTTGTGTTCAAGTGCTTTTT+TGG + tig0023079:75356-75375 None:intergenic 35.0%
AAAGCAATTGCAAATCGTCT+TGG - tig0023079:75376-75395 Msa1404730:three_prime_UTR 35.0%
CAATACGAAGATGTAATAGC+CGG - tig0023079:75421-75440 Msa1404730:exon 35.0%
GTCAGGATTTATAACATCAC+CGG + tig0023079:75443-75462 None:intergenic 35.0%
GATGTTGAGTTTGACAGTAT+TGG - tig0023079:75469-75488 Msa1404730:CDS 35.0%
AAACTATTGGGTCCTCAAAA+GGG - tig0023079:75571-75590 Msa1404730:CDS 35.0%
TATCCCCAAAATCTTGCAAA+TGG - tig0023079:75850-75869 Msa1404730:intron 35.0%
! AGTCCATTTGCAAGATTTTG+GGG + tig0023079:75856-75875 None:intergenic 35.0%
! GAAAAGGTTAATATTGGCCT+GGG + tig0023079:75954-75973 None:intergenic 35.0%
AGAAAAGGTTAATATTGGCC+TGG + tig0023079:75955-75974 None:intergenic 35.0%
GTTTGTCTTGTCGTTCAAAA+AGG + tig0023079:75994-76013 None:intergenic 35.0%
TTAGACACACAGCAACATAT+TGG + tig0023079:76081-76100 None:intergenic 35.0%
ATATGTTGCTGTGTGTCTAA+TGG - tig0023079:76081-76100 Msa1404730:intron 35.0%
AACCATTACGGATCTATTGT+AGG + tig0023079:76175-76194 None:intergenic 35.0%
!!! GGTTGCCTTTTTTATCATTG+AGG - tig0023079:76234-76253 Msa1404730:intron 35.0%
AAGGCCATCTGTTTATCTAA+TGG - tig0023079:76359-76378 Msa1404730:CDS 35.0%
TAGAAACCAGTTCCAATATC+AGG + tig0023079:76501-76520 None:intergenic 35.0%
! TGATATTGGAACTGGTTTCT+AGG - tig0023079:76500-76519 Msa1404730:CDS 35.0%
ATGTGTGGTTATTTGGTTGA+AGG - tig0023079:76691-76710 Msa1404730:intron 35.0%
!! ACTACTTTAGAACTTACGGT+CGG + tig0023079:77059-77078 None:intergenic 35.0%
!! TTGCTTCATCAAGTTCTGAA+GGG + tig0023079:77219-77238 None:intergenic 35.0%
! ATTGCTTCATCAAGTTCTGA+AGG + tig0023079:77220-77239 None:intergenic 35.0%
! TTTTCCTATTAGAGAACTGC+TGG - tig0023079:77423-77442 Msa1404730:intron 35.0%
AACAGAAACTGAAACAGATG+TGG + tig0023079:77591-77610 None:intergenic 35.0%
! CACAAATCTAAAACTTAGGG+GGG + tig0023079:77652-77671 None:intergenic 35.0%
! GCACAAATCTAAAACTTAGG+GGG + tig0023079:77653-77672 None:intergenic 35.0%
! CGCACAAATCTAAAACTTAG+GGG + tig0023079:77654-77673 None:intergenic 35.0%
ATCATAAAGGGGCTAAACTT+GGG + tig0023079:77687-77706 None:intergenic 35.0%
AATCATAAAGGGGCTAAACT+TGG + tig0023079:77688-77707 None:intergenic 35.0%
AGGGGCTACAAAATCATAAA+GGG + tig0023079:77699-77718 None:intergenic 35.0%
ATTAGGGAAAAACATGTGAG+GGG + tig0023079:77717-77736 None:intergenic 35.0%
! TCCCTAATTTTGAGCTGATA+CGG - tig0023079:77729-77748 Msa1404730:intron 35.0%
! AAATTAGGGAAAAGCATGTG+AGG - tig0023079:77941-77960 Msa1404730:intron 35.0%
! AATTAGGGAAAAGCATGTGA+GGG - tig0023079:77942-77961 Msa1404730:intron 35.0%
! AGTCTTGTACTCTTGTTAAC+TGG - tig0023079:78049-78068 Msa1404730:CDS 35.0%
TCAGTAATTTCGATCACCAA+AGG + tig0023079:78079-78098 None:intergenic 35.0%
TCTAAATCAAACTGCGACAA+AGG + tig0023079:78139-78158 None:intergenic 35.0%
! TACTTTCGTTTAGCTTAGTC+CGG - tig0023079:78365-78384 Msa1404730:CDS 35.0%
!! TGTTGTTTACAAAAGTGCCA+AGG - tig0023079:78460-78479 Msa1404730:CDS 35.0%
AGAAAACGAATCTCCATGCT+TGG + tig0023079:75144-75163 None:intergenic 40.0%
TTCTTCTCGGTAGAAGAGAA+CGG - tig0023079:75160-75179 Msa1404730:three_prime_UTR 40.0%
CGGGTCAAGATATGAATAAC+CGG + tig0023079:75320-75339 None:intergenic 40.0%
! TTTTGGATGATTGACGGTTC+GGG + tig0023079:75339-75358 None:intergenic 40.0%
CGGCTATTACATCTTCGTAT+TGG + tig0023079:75423-75442 None:intergenic 40.0%
TGTCAAACTCAACATCGATG+TGG + tig0023079:75465-75484 None:intergenic 40.0%
! GTTGAGTTTGACAGTATTGG+AGG - tig0023079:75472-75491 Msa1404730:CDS 40.0%
! GTTTGACAGTATTGGAGGAT+TGG - tig0023079:75477-75496 Msa1404730:CDS 40.0%
GATCAGAATTGTTTGCCCAT+GGG - tig0023079:75548-75567 Msa1404730:CDS 40.0%
TTTGCCCATGGGAAACTATT+GGG - tig0023079:75559-75578 Msa1404730:CDS 40.0%
GAAACTATTGGGTCCTCAAA+AGG - tig0023079:75570-75589 Msa1404730:CDS 40.0%
! TACAACAAGACACCCTTTTG+AGG + tig0023079:75586-75605 None:intergenic 40.0%
!! CAAAAGGGTGTCTTGTTGTA+TGG - tig0023079:75586-75605 Msa1404730:CDS 40.0%
!! AGCAATAGCTTTAGCAAGCA+TGG + tig0023079:75632-75651 None:intergenic 40.0%
! GCTTGCTAAAGCTATTGCTA+AGG - tig0023079:75633-75652 Msa1404730:intron 40.0%
AAAGCTATTGCTAAGGAGTG+TGG - tig0023079:75640-75659 Msa1404730:intron 40.0%
GAGCGGCTTTCATTAATGTT+AGG - tig0023079:75662-75681 Msa1404730:intron 40.0%
TGTCTAATCTCATGAGCATG+TGG - tig0023079:75686-75705 Msa1404730:intron 40.0%
AATCTCATGAGCATGTGGTT+CGG - tig0023079:75691-75710 Msa1404730:intron 40.0%
!! AGCCGGTTTTATAGCAGAAA+AGG + tig0023079:75970-75989 None:intergenic 40.0%
TTGTCGTTCAAAAAGGTAGC+CGG + tig0023079:75987-76006 None:intergenic 40.0%
TGCCTACAATAGATCCGTAA+TGG - tig0023079:76170-76189 Msa1404730:intron 40.0%
!! GGAAAGGTTCTGAACCATTA+CGG + tig0023079:76187-76206 None:intergenic 40.0%
AATGCCCATAAATCGATCGA+GGG + tig0023079:76455-76474 None:intergenic 40.0%
AAATGCCCATAAATCGATCG+AGG + tig0023079:76456-76475 None:intergenic 40.0%
TTAGCTGCTAAGCCTGATAT+TGG - tig0023079:76486-76505 Msa1404730:CDS 40.0%
!! AATATTTAACGAGGGCATCC+TGG - tig0023079:76756-76775 Msa1404730:CDS 40.0%
GAAGCATAGTTATGTATGCC+AGG + tig0023079:76777-76796 None:intergenic 40.0%
AGTGTCTGCCATATTTAGCT+TGG - tig0023079:76901-76920 Msa1404730:intron 40.0%
! AAACTGAGTTCATGGCTCTA+TGG - tig0023079:77020-77039 Msa1404730:intron 40.0%
! AACTGAGTTCATGGCTCTAT+GGG - tig0023079:77021-77040 Msa1404730:intron 40.0%
TAACTCGAGCATTCTCTACA+CGG + tig0023079:77174-77193 None:intergenic 40.0%
TGTAGAGAATGCTCGAGTTA+TGG - tig0023079:77174-77193 Msa1404730:intron 40.0%
!! TTTCCTCAAGCCTTTGAAGT+TGG - tig0023079:77250-77269 Msa1404730:intron 40.0%
ATTCCAACTTCAAAGGCTTG+AGG + tig0023079:77256-77275 None:intergenic 40.0%
! GTCTGGAATTCCAACTTCAA+AGG + tig0023079:77263-77282 None:intergenic 40.0%
AATTCCAGACCAGAAAGAGA+GGG - tig0023079:77273-77292 Msa1404730:intron 40.0%
ATATCTCAACCCTCTCTTTC+TGG + tig0023079:77285-77304 None:intergenic 40.0%
AGAGAGGGTTGAGATATTGA+AGG - tig0023079:77288-77307 Msa1404730:intron 40.0%
TCATCCAGCAGTTCTCTAAT+AGG + tig0023079:77430-77449 None:intergenic 40.0%
TCATAAAGGGGCTAAACTTG+GGG + tig0023079:77686-77705 None:intergenic 40.0%
GGGGCTACAAAATCATAAAG+GGG + tig0023079:77698-77717 None:intergenic 40.0%
GAGGGGCTACAAAATCATAA+AGG + tig0023079:77700-77719 None:intergenic 40.0%
GCCGTATCAGCTCAAAATTA+GGG + tig0023079:77733-77752 None:intergenic 40.0%
TGCCGTATCAGCTCAAAATT+AGG + tig0023079:77734-77753 None:intergenic 40.0%
AAATGCAGAACTGAAGGGAA+AGG + tig0023079:77857-77876 None:intergenic 40.0%
CAAGCAAATGCAGAACTGAA+GGG + tig0023079:77862-77881 None:intergenic 40.0%
ACAAGCAAATGCAGAACTGA+AGG + tig0023079:77863-77882 None:intergenic 40.0%
! ATTAGGGAAAAGCATGTGAG+GGG - tig0023079:77943-77962 Msa1404730:intron 40.0%
AGGGGCTACAAAATCATGAA+GGG - tig0023079:77961-77980 Msa1404730:intron 40.0%
! TCATCAAGACGAACGTTTTC+TGG - tig0023079:78214-78233 Msa1404730:CDS 40.0%
TGGTGAGTCAGTTGATTATC+AGG - tig0023079:78234-78253 Msa1404730:CDS 40.0%
GGTGAGTCAGTTGATTATCA+GGG - tig0023079:78235-78254 Msa1404730:CDS 40.0%
AAGAATCCTGGGAATCTTGT+TGG + tig0023079:78315-78334 None:intergenic 40.0%
AAGTAATCGCTGTTGCAATC+CGG + tig0023079:78387-78406 None:intergenic 40.0%
!! GTTTACAAAAGTGCCAAGGT+TGG - tig0023079:78464-78483 Msa1404730:CDS 40.0%
AAACGAATCTCCATGCTTGG+AGG + tig0023079:75141-75160 None:intergenic 45.0%
AACTCAACATCGATGTGGTC+AGG + tig0023079:75460-75479 None:intergenic 45.0%
CGATCAGAATTGTTTGCCCA+TGG - tig0023079:75547-75566 Msa1404730:CDS 45.0%
GTTTGCCCATGGGAAACTAT+TGG - tig0023079:75558-75577 Msa1404730:CDS 45.0%
AGGACCCAATAGTTTCCCAT+GGG + tig0023079:75566-75585 None:intergenic 45.0%
! TATTGCTAAGGAGTGTGGAG+CGG - tig0023079:75645-75664 Msa1404730:intron 45.0%
!! GTCTGCGCTATTTCTGAATG+TGG - tig0023079:75735-75754 Msa1404730:intron 45.0%
CTTTGATGTATCTATGGCCC+AGG - tig0023079:75934-75953 Msa1404730:intron 45.0%
TGCTGTGTGTCTAATGGTCA+CGG - tig0023079:76087-76106 Msa1404730:intron 45.0%
CTACGAAATTCCACTGTGGT+GGG + tig0023079:76215-76234 None:intergenic 45.0%
CAACCTACGAAATTCCACTG+TGG + tig0023079:76219-76238 None:intergenic 45.0%
GCTAAGCCTGATATTGGAAC+TGG - tig0023079:76492-76511 Msa1404730:CDS 45.0%
!! TGGAACTGGTTTCTAGGATG+AGG - tig0023079:76506-76525 Msa1404730:CDS 45.0%
TCCTGAACGAGGAACATACA+GGG + tig0023079:76612-76631 None:intergenic 45.0%
TACAGATACACTCCTGAACG+AGG + tig0023079:76623-76642 None:intergenic 45.0%
GGAGTGTATCTGTATAGCGA+TGG - tig0023079:76629-76648 Msa1404730:intron 45.0%
! AGTTCATGGCTCTATGGGAT+GGG - tig0023079:77026-77045 Msa1404730:intron 45.0%
GAATTCCAGACCAGAAAGAG+AGG - tig0023079:77272-77291 Msa1404730:intron 45.0%
AGGTTATCTTAAAGGGCGAG+AGG - tig0023079:77308-77327 Msa1404730:intron 45.0%
GGTTATCTTAAAGGGCGAGA+GGG - tig0023079:77309-77328 Msa1404730:intron 45.0%
GGTTTATGCAAGGGTTACAC+AGG - tig0023079:77364-77383 Msa1404730:intron 45.0%
TGCTGGATGACGAGAAGAAA+GGG - tig0023079:77440-77459 Msa1404730:intron 45.0%
AATATGCTGTGCACAGCTGT+TGG - tig0023079:77543-77562 Msa1404730:intron 45.0%
ATAAAGGGGCTAAACTTGGG+GGG + tig0023079:77684-77703 None:intergenic 45.0%
CATAAAGGGGCTAAACTTGG+GGG + tig0023079:77685-77704 None:intergenic 45.0%
!! GCTTGCTATGTTTGCCATAG+TGG - tig0023079:77772-77791 Msa1404730:intron 45.0%
GAGGGGCTACAAAATCATGA+AGG - tig0023079:77960-77979 Msa1404730:intron 45.0%
AGAATGTTGTGCTGGATGTG+TGG - tig0023079:77998-78017 Msa1404730:intron 45.0%
TCAAACTGCGACAAAGGTCT+AGG + tig0023079:78133-78152 None:intergenic 45.0%
AGAATGCCCTTTCCACTTCA+CGG - tig0023079:78158-78177 Msa1404730:CDS 45.0%
TAACCTCCAACAAGATTCCC+AGG - tig0023079:78306-78325 Msa1404730:CDS 45.0%
AATCCTGGGAATCTTGTTGG+AGG + tig0023079:78312-78331 None:intergenic 45.0%
CAAAATGCAACAGCCAACCT+TGG + tig0023079:78480-78499 None:intergenic 45.0%
!!! TTTTTTTTATAAAAAAAAAC+TGG - tig0023079:77810-77829 Msa1404730:intron 5.0%
ACTAACTCCTCCTCCAAGCA+TGG - tig0023079:75128-75147 Msa1404730:three_prime_UTR 50.0%
GGTAGAAGAGAACGGTTCTG+CGG - tig0023079:75168-75187 Msa1404730:three_prime_UTR 50.0%
! GATTTTATTAGCGGCGAGCG+CGG - tig0023079:75270-75289 Msa1404730:intron 50.0%
GAGGACCCAATAGTTTCCCA+TGG + tig0023079:75567-75586 None:intergenic 50.0%
! GTCTTGTTGTATGGACCACC+TGG - tig0023079:75595-75614 Msa1404730:CDS 50.0%
TTGTATGGACCACCTGGTAC+TGG - tig0023079:75601-75620 Msa1404730:CDS 50.0%
!! ATGGTTTTGCCAGTACCAGG+TGG + tig0023079:75613-75632 None:intergenic 50.0%
!! AGCATGGTTTTGCCAGTACC+AGG + tig0023079:75616-75635 None:intergenic 50.0%
TTCGGCGATGCAACTAAGCT+TGG - tig0023079:75709-75728 Msa1404730:intron 50.0%
! ACTGTGGTGGGATCTAGGAA+AGG + tig0023079:76203-76222 None:intergenic 50.0%
TTTCCTAGATCCCACCACAG+TGG - tig0023079:76202-76221 Msa1404730:intron 50.0%
! ATTCCACTGTGGTGGGATCT+AGG + tig0023079:76208-76227 None:intergenic 50.0%
CCTACGAAATTCCACTGTGG+TGG + tig0023079:76216-76235 None:intergenic 50.0%
CCACCACAGTGGAATTTCGT+AGG - tig0023079:76213-76232 Msa1404730:intron 50.0%
AAACCTGCAGGGAGTTCAGT+CGG + tig0023079:76283-76302 None:intergenic 50.0%
! TCCCTCCCTCGATCGATTTA+TGG - tig0023079:76447-76466 Msa1404730:CDS 50.0%
CCCATAAATCGATCGAGGGA+GGG + tig0023079:76451-76470 None:intergenic 50.0%
! CCCTCCCTCGATCGATTTAT+GGG - tig0023079:76448-76467 Msa1404730:CDS 50.0%
TCCCTGTATGTTCCTCGTTC+AGG - tig0023079:76608-76627 Msa1404730:intron 50.0%
CTCCTGAACGAGGAACATAC+AGG + tig0023079:76613-76632 None:intergenic 50.0%
ATAGCGATGGCTACCCTTGT+TGG - tig0023079:76642-76661 Msa1404730:intron 50.0%
GAAGCTGTTTGTGCGCACAT+AGG - tig0023079:76717-76736 Msa1404730:CDS 50.0%
ACGTCGTTCATCAGAACACG+AGG - tig0023079:76982-77001 Msa1404730:intron 50.0%
! GAGTTCATGGCTCTATGGGA+TGG - tig0023079:77025-77044 Msa1404730:intron 50.0%
! TCAACCCTCTCTTTCTGGTC+TGG + tig0023079:77280-77299 None:intergenic 50.0%
CTGCTGGATGACGAGAAGAA+AGG - tig0023079:77439-77458 Msa1404730:intron 50.0%
GATGTGTGGCGAGACTTGAT+GGG - tig0023079:78012-78031 Msa1404730:intron 50.0%
CTTGTTAACTGGCGTGCCTT+TGG - tig0023079:78060-78079 Msa1404730:CDS 50.0%
CGAATCTCCATGCTTGGAGG+AGG + tig0023079:75138-75157 None:intergenic 55.0%
TCTAATGGTCACGGCCTCAC+AGG - tig0023079:76096-76115 Msa1404730:intron 55.0%
TTTCCGACTGAACTCCCTGC+AGG - tig0023079:76277-76296 Msa1404730:intron 55.0%
GCGCGTCAAACAAACCTGCA+GGG + tig0023079:76294-76313 None:intergenic 55.0%
TGCGCGTCAAACAAACCTGC+AGG + tig0023079:76295-76314 None:intergenic 55.0%
GCCCATAAATCGATCGAGGG+AGG + tig0023079:76452-76471 None:intergenic 55.0%
!! CAGCTGTTGGCTGACTTGAC+TGG - tig0023079:77556-77575 Msa1404730:intron 55.0%
AGCACAACATTCTGCTCGCG+TGG + tig0023079:77991-78010 None:intergenic 55.0%
ACGCGAGCAGAATGTTGTGC+TGG - tig0023079:77990-78009 Msa1404730:intron 55.0%
GGATGTGTGGCGAGACTTGA+TGG - tig0023079:78011-78030 Msa1404730:intron 55.0%
CGTCGTCCGTGAAGTGGAAA+GGG + tig0023079:78167-78186 None:intergenic 55.0%
CCTTTCCACTTCACGGACGA+CGG - tig0023079:78165-78184 Msa1404730:CDS 55.0%
ACGGCTGTTGCAAGCGAGTA+CGG - tig0023079:78184-78203 Msa1404730:CDS 55.0%
TTCCACGACGTGCACCTGTG+AGG + tig0023079:76113-76132 None:intergenic 60.0%
CCGTCGTCCGTGAAGTGGAA+AGG + tig0023079:78168-78187 None:intergenic 60.0%
AACAGCCGTCGTCCGTGAAG+TGG + tig0023079:78173-78192 None:intergenic 60.0%
GGCCTCACAGGTGCACGTCG+TGG - tig0023079:76108-76127 Msa1404730:intron 70.0%
Chromosome Type Strat End Strand Name
tig0023079 gene 75100 78670 75100 ID=Msa1404730;Name=Msa1404730
tig0023079 mRNA 75100 78670 75100 ID=Msa1404730-mRNA-1;Parent=Msa1404730;Name=Msa1404730-mRNA-1;_AED=0.31;_eAED=0.31;_QI=126|1|1|1|0.75|0.6|5|308|389
tig0023079 exon 75100 75274 75100 ID=Msa1404730-mRNA-1:exon:23771;Parent=Msa1404730-mRNA-1
tig0023079 exon 75301 75643 75301 ID=Msa1404730-mRNA-1:exon:23770;Parent=Msa1404730-mRNA-1
tig0023079 exon 76296 76591 76296 ID=Msa1404730-mRNA-1:exon:23769;Parent=Msa1404730-mRNA-1
tig0023079 exon 76709 76867 76709 ID=Msa1404730-mRNA-1:exon:23768;Parent=Msa1404730-mRNA-1
tig0023079 exon 78040 78670 78040 ID=Msa1404730-mRNA-1:exon:23767;Parent=Msa1404730-mRNA-1
tig0023079 five_prime_UTR 78545 78670 78545 ID=Msa1404730-mRNA-1:five_prime_utr;Parent=Msa1404730-mRNA-1
tig0023079 CDS 78040 78544 78040 ID=Msa1404730-mRNA-1:cds;Parent=Msa1404730-mRNA-1
tig0023079 CDS 76709 76867 76709 ID=Msa1404730-mRNA-1:cds;Parent=Msa1404730-mRNA-1
tig0023079 CDS 76296 76591 76296 ID=Msa1404730-mRNA-1:cds;Parent=Msa1404730-mRNA-1
tig0023079 CDS 75434 75643 75434 ID=Msa1404730-mRNA-1:cds;Parent=Msa1404730-mRNA-1
tig0023079 three_prime_UTR 75301 75433 75301 ID=Msa1404730-mRNA-1:three_prime_utr;Parent=Msa1404730-mRNA-1
tig0023079 three_prime_UTR 75100 75274 75100 ID=Msa1404730-mRNA-1:three_prime_utr;Parent=Msa1404730-mRNA-1
Gene Sequence

>Msa1404730

ATGTCGAATTCACCAGAATCAGAATCGAAGTTTGTAACTGATTTGATTTTATTAGCGGCGAGCGCGGCATTCAGTTGCTTAATATTGTACACCGGTTATTCATATCTTGACCCGAACCGTCAATCATCCAAAAAAGCACTTGAACACAAGAAAGCAATTGCAAATCGTCTTGGTCGTCCCCTCATTAAAACAAACCAATACGAAGATGTAATAGCCGGTGATGTTATAAATCCTGACCACATCGATGTTGAGTTTGACAGTATTGGAGGATTGGAAGCTATTAAAGAAGCTTTGTTTGAGCTTGTTATTCTTCCTTTGAAACGATCAGAATTGTTTGCCCATGGGAAACTATTGGGTCCTCAAAAGGGTGTCTTGTTGTATGGACCACCTGGTACTGGCAAAACCATGCTTGCTAAAGCTATTGCTAAGGAGTGTGGAGCGGCTTTCATTAATGTTAGGATGTCTAATCTCATGAGCATGTGGTTCGGCGATGCAACTAAGCTTGTGTCTGCCATATTTAGCTTGGCTTATAAGCTTCAGCCTGCTATCATATTCATTGATGAAGTAGACAGTTTTTTGGGTCAACGTCGTTCATCAGAACACGAGGCTTCGCTAAATATGAAAACTGAGTTCATGGCTCTATGGGATGGGTTCAGCACCGACCAGAATGCTCGAGTTATGGTACTTGCAGCAACTAATCGCCCTTCAGAACTTGATGAAGCAATACTTCGGCGTTTTCCTCAAGCCTTTGAAGTTGGAATTCCAGACCAGAAAGAGAGGGTTGAGATATTGAAGGTTATCTTAAAGGGCGAGAGGGTTGAAGATAACATAGACTTCAATTATATAGCTGGTTTATGCAAGGGTTACACAGGTTCAGATCTATTTGATCTTTGCAAGAAGGCTGCATATTTTCCTATTAGAGAACTGCTGGATGACGAGAAGAAAGGGAAACAATCTCAAGAACCTAGACCTTTGTCGCAGTTTGATTTAGAGAATGCCCTTTCCACTTCACGGACGACGGCTGTTGCAAGCGAGTACGGCGTGTCATCATCAAGACGAACGTTTTCTGGTGAGTCAGTTGATTATCAGGGTCAAGCTGCAATCAATGAATTCTCTAAGCTTATGGTTTCTTATATGCTTAACCTCCAACAAGATTCCCAGGATTCTTAA
Protein sequence

>Msa1404730

MSNSPESESKFVTDLILLAASAAFSCLILYTGYSYLDPNRQSSKKALEHKKAIANRLGRPLIKTNQYEDVIAGDVINPDHIDVEFDSIGGLEAIKEALFELVILPLKRSELFAHGKLLGPQKGVLLYGPPGTGKTMLAKAIAKECGAAFINVRMSNLMSMWFGDATKLVSAIFSLAYKLQPAIIFIDEVDSFLGQRRSSEHEASLNMKTEFMALWDGFSTDQNARVMVLAATNRPSELDEAILRRFPQAFEVGIPDQKERVEILKVILKGERVEDNIDFNYIAGLCKGYTGSDLFDLCKKAAYFPIRELLDDEKKGKQSQEPRPLSQFDLENALSTSRTTAVASEYGVSSSRRTFSGESVDYQGQAAINEFSKLMVSYMLNLQQDSQDS*