Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0473320 | XP_003602469.2 | 98.656 | 744 | 10 | 0 | 1 | 744 | 1 | 744 | 0.0 | 1500 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0473320 | sp|Q93Y08|AB1K8_ARATH | 79.945 | 733 | 126 | 8 | 23 | 744 | 39 | 761 | 0.0 | 1134 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000220 | Msa0473320 | 0.857813 | 1.264152e-62 | -8.615850e-47 |
| Msa0001040 | Msa0473320 | 0.800739 | 1.251405e-48 | -8.615850e-47 |
| Msa0002690 | Msa0473320 | 0.817376 | 3.404916e-52 | -8.615850e-47 |
| Msa0002700 | Msa0473320 | 0.819699 | 1.012743e-52 | -8.615850e-47 |
| Msa0003370 | Msa0473320 | 0.814759 | 1.308316e-51 | -8.615850e-47 |
| Msa0005160 | Msa0473320 | 0.823294 | 1.496449e-53 | -8.615850e-47 |
| Msa0005500 | Msa0473320 | 0.823407 | 1.408779e-53 | -8.615850e-47 |
| Msa0006820 | Msa0473320 | 0.800121 | 1.671696e-48 | -8.615850e-47 |
| Msa0007160 | Msa0473320 | 0.849822 | 2.525996e-60 | -8.615850e-47 |
| Msa0009210 | Msa0473320 | 0.830409 | 2.984946e-55 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0473320 | MtrunA17_Chr3g0129101 | 98.656 | 744 | 10 | 0 | 1 | 744 | 1 | 744 | 0.0 | 1500 |
| Msa0473320 | MtrunA17_Chr8g0382921 | 84.783 | 736 | 98 | 5 | 12 | 744 | 11 | 735 | 0.0 | 1262 |
| Msa0473320 | MtrunA17_Chr3g0120791 | 46.281 | 605 | 284 | 9 | 143 | 740 | 138 | 708 | 0.0 | 539 |
| Msa0473320 | MtrunA17_Chr4g0012061 | 33.628 | 452 | 256 | 9 | 188 | 631 | 140 | 555 | 1.07e-73 | 253 |
| Msa0473320 | MtrunA17_Chr7g0230671 | 34.375 | 480 | 264 | 11 | 161 | 631 | 106 | 543 | 1.34e-73 | 253 |
| Msa0473320 | MtrunA17_Chr3g0136721 | 34.940 | 415 | 223 | 10 | 207 | 608 | 138 | 518 | 8.50e-69 | 239 |
| Msa0473320 | MtrunA17_Chr6g0465161 | 34.398 | 407 | 220 | 9 | 204 | 606 | 129 | 492 | 1.64e-66 | 233 |
| Msa0473320 | MtrunA17_Chr1g0148121 | 31.868 | 455 | 267 | 7 | 185 | 631 | 116 | 535 | 6.38e-64 | 225 |
| Msa0473320 | MtrunA17_Chr1g0199921 | 34.917 | 421 | 224 | 9 | 207 | 611 | 203 | 589 | 7.75e-59 | 214 |
| Msa0473320 | MtrunA17_Chr1g0169361 | 31.894 | 417 | 242 | 9 | 195 | 606 | 173 | 552 | 4.54e-54 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0473320 | AT5G64940.1 | 79.945 | 733 | 126 | 8 | 23 | 744 | 39 | 761 | 0.0 | 1134 |
| Msa0473320 | AT5G64940.2 | 79.945 | 733 | 126 | 8 | 23 | 744 | 39 | 761 | 0.0 | 1134 |
| Msa0473320 | AT3G07700.1 | 45.521 | 681 | 298 | 16 | 71 | 740 | 77 | 695 | 0.0 | 554 |
| Msa0473320 | AT3G07700.2 | 45.521 | 681 | 298 | 16 | 71 | 740 | 77 | 695 | 0.0 | 554 |
| Msa0473320 | AT3G07700.4 | 45.521 | 681 | 298 | 16 | 71 | 740 | 77 | 695 | 0.0 | 554 |
| Msa0473320 | AT3G07700.3 | 44.913 | 688 | 321 | 17 | 71 | 740 | 77 | 724 | 0.0 | 550 |
| Msa0473320 | AT1G79600.1 | 33.267 | 499 | 278 | 11 | 156 | 645 | 108 | 560 | 2.94e-76 | 260 |
| Msa0473320 | AT5G24970.3 | 33.623 | 461 | 240 | 10 | 200 | 631 | 134 | 557 | 3.64e-71 | 247 |
| Msa0473320 | AT5G24970.2 | 33.623 | 461 | 240 | 10 | 200 | 631 | 173 | 596 | 9.75e-71 | 246 |
| Msa0473320 | AT4G31390.1 | 28.837 | 645 | 374 | 17 | 49 | 671 | 19 | 600 | 5.01e-68 | 237 |
Find 219 sgRNAs with CRISPR-Local
Find 1113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAGGTTGGAGGAGAT+TGG | 0.383706 | 3_3:-75911052 | Msa0473320:CDS |
| AAAATTGATCCAAAGTCAGA+TGG | 0.579221 | 3_3:-75907516 | Msa0473320:CDS |
| AAACAACGTCTAGCTGCTAT+TGG | 0.409897 | 3_3:-75901355 | Msa0473320:intron |
| AAACGGTGTAGCTTCTACGG+TGG | 0.670317 | 3_3:-75911116 | Msa0473320:CDS |
| AAAGAGAAAGAGGAAGCAAC+AGG | 0.432434 | 3_3:-75901430 | Msa0473320:CDS |
| AAAGCACTCTCAGAGGGAAA+TGG | 0.437529 | 3_3:+75908931 | None:intergenic |
| AAAGCGGGAAATGGAGCGTG+TGG | 0.632968 | 3_3:+75911327 | None:intergenic |
| AAAGGAAATGGAGTTCGGGT+AGG | 0.601045 | 3_3:+75911386 | None:intergenic |
| AAAGTCAGATGGTGCAAAGA+GGG | 0.557547 | 3_3:-75907505 | Msa0473320:CDS |
| AAATACAACATGCATATTGA+TGG | 0.358961 | 3_3:+75896764 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACGGAAGTTCAGTAAAATA+TGG | - | chr3_3:75897139-75897158 | Msa0473320:three_prime_UTR | 30.0% | |
| AACAAATCCCCAAATTTCTA+AGG | + | chr3_3:75897624-75897643 | None:intergenic | 30.0% | |
| TGTTGTAACCTTCATCTTTA+AGG | - | chr3_3:75897640-75897659 | Msa0473320:intron | 30.0% | |
| AATCAGAAGTTCTCTTACAA+AGG | - | chr3_3:75897674-75897693 | Msa0473320:intron | 30.0% | |
| TAGATTTGTGGTTTATGCAT+AGG | - | chr3_3:75897817-75897836 | Msa0473320:intron | 30.0% | |
| TGATAAGTAAGTGATGTGAT+AGG | - | chr3_3:75897916-75897935 | Msa0473320:intron | 30.0% | |
| AGAGAGATAGAGAGAAAATA+AGG | - | chr3_3:75897940-75897959 | Msa0473320:intron | 30.0% | |
| AATGAGTGTAGGAAAATCAA+AGG | - | chr3_3:75897968-75897987 | Msa0473320:intron | 30.0% | |
| TTGAAGATATTACTTACAGG+AGG | - | chr3_3:75898093-75898112 | Msa0473320:intron | 30.0% | |
| AGGAAAAAAAGAAGTTAAGG+CGG | - | chr3_3:75898124-75898143 | Msa0473320:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 75896718 | 75911588 | 75896718 | ID=Msa0473320;Name=Msa0473320 |
| chr3_3 | mRNA | 75896718 | 75911588 | 75896718 | ID=Msa0473320-mRNA-1;Parent=Msa0473320;Name=Msa0473320-mRNA-1;_AED=0.12;_eAED=0.12;_QI=154|0.95|0.90|1|0.95|0.90|21|634|744 |
| chr3_3 | exon | 75896718 | 75897340 | 75896718 | ID=Msa0473320-mRNA-1:exon:19814;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75899144 | 75899271 | 75899144 | ID=Msa0473320-mRNA-1:exon:19813;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75899413 | 75899472 | 75899413 | ID=Msa0473320-mRNA-1:exon:19812;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75900109 | 75900198 | 75900109 | ID=Msa0473320-mRNA-1:exon:19811;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75900312 | 75900410 | 75900312 | ID=Msa0473320-mRNA-1:exon:19810;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75900495 | 75900549 | 75900495 | ID=Msa0473320-mRNA-1:exon:19809;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75900657 | 75900717 | 75900657 | ID=Msa0473320-mRNA-1:exon:19808;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75900799 | 75900879 | 75900799 | ID=Msa0473320-mRNA-1:exon:19807;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75901356 | 75901480 | 75901356 | ID=Msa0473320-mRNA-1:exon:19806;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75904177 | 75904265 | 75904177 | ID=Msa0473320-mRNA-1:exon:19805;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75904390 | 75904459 | 75904390 | ID=Msa0473320-mRNA-1:exon:19804;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75904587 | 75904660 | 75904587 | ID=Msa0473320-mRNA-1:exon:19803;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75906002 | 75906071 | 75906002 | ID=Msa0473320-mRNA-1:exon:19802;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75906190 | 75906269 | 75906190 | ID=Msa0473320-mRNA-1:exon:19801;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75906377 | 75906487 | 75906377 | ID=Msa0473320-mRNA-1:exon:19800;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75907461 | 75907559 | 75907461 | ID=Msa0473320-mRNA-1:exon:19799;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75907820 | 75907920 | 75907820 | ID=Msa0473320-mRNA-1:exon:19798;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75908845 | 75908965 | 75908845 | ID=Msa0473320-mRNA-1:exon:19797;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75910028 | 75910194 | 75910028 | ID=Msa0473320-mRNA-1:exon:19796;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75910611 | 75910746 | 75910611 | ID=Msa0473320-mRNA-1:exon:19795;Parent=Msa0473320-mRNA-1 |
| chr3_3 | exon | 75911006 | 75911588 | 75911006 | ID=Msa0473320-mRNA-1:exon:19794;Parent=Msa0473320-mRNA-1 |
| chr3_3 | five_prime_UTR | 75911435 | 75911588 | 75911435 | ID=Msa0473320-mRNA-1:five_prime_utr;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75911006 | 75911434 | 75911006 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75910611 | 75910746 | 75910611 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75910028 | 75910194 | 75910028 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75908845 | 75908965 | 75908845 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75907820 | 75907920 | 75907820 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75907461 | 75907559 | 75907461 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75906377 | 75906487 | 75906377 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75906190 | 75906269 | 75906190 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75906002 | 75906071 | 75906002 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75904587 | 75904660 | 75904587 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75904390 | 75904459 | 75904390 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75904177 | 75904265 | 75904177 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75901356 | 75901480 | 75901356 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75900799 | 75900879 | 75900799 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75900657 | 75900717 | 75900657 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75900495 | 75900549 | 75900495 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75900312 | 75900410 | 75900312 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75900109 | 75900198 | 75900109 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75899413 | 75899472 | 75899413 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | CDS | 75899155 | 75899271 | 75899155 | ID=Msa0473320-mRNA-1:cds;Parent=Msa0473320-mRNA-1 |
| chr3_3 | three_prime_UTR | 75899144 | 75899154 | 75899144 | ID=Msa0473320-mRNA-1:three_prime_utr;Parent=Msa0473320-mRNA-1 |
| chr3_3 | three_prime_UTR | 75896718 | 75897340 | 75896718 | ID=Msa0473320-mRNA-1:three_prime_utr;Parent=Msa0473320-mRNA-1 |
| Gene Sequence |
>Msa0473320 ATGGCTACTACCTCTTCTCTTCTTCACCTACCCGAACTCCATTTCCTTTCTCCACAAATCACTCTCCGCAACCGTTTATCTTTCTCCACACGCTCCATTTCCCGCTTTTCTACCTCCAATGTCACTCTTCGCGCCACACGAATCCGCTCTTCAAAAGATGAATCCGCGATAGCAGAACGAGTTAACGACGTGGAATGGAGCGGAAACGGAGTTGCCTCCCGTGCCGGCCGTAATGGAGCAGCAGGAGGTTATGTTAACGGCGCCGTTAACGGAAGTTCAGTAAAATATGGAAATGGAAACGGTGTAGCTTCTACGGTGGTGGTGGATGTTGAAGCTTCTAAGGTGAACGAGGATGGAAGGAAAAGAAGGTTGGAGGAGATTGGAAAAGAAGACGCGTGGTTTAAACAAACTGAGGAACAAAAAGTTGAGGTAGCCGTTGCTCCTGGAGGTCGTTGGAGTAGATTCAAGACTTACTCTACAATCCAGAGAACCTTAGAAATTTGGGGATTTGTTGTAACCTTCATCTTTAAGGCTTGGTTGGACAATCAGAAGTTCTCTTACAAAGGAGGAATGACCGAGGAAAAAAAGAAGTTAAGGCGGAAGACCCTTGCCAAGTGGCTTAAGGAAAGCATCTTGAGATTAGGTCCTACATTCATTAAAATTGGGCAGCAGTTCTCCACTAGAGTGGACATTCTTCCTCAAGAATATGTTGACCAGTTGTCTGAACTTCAGGATCAAGTTCCTCCATTTCCCTCTGAGAGTGCTTTAGCTATTGTTGAAGAAGAGCTTGGAGCTTCTGTAGGTGATATCTTTGATCAGTTTGACTATGAACCAATAGCTGCTGCAAGTCTTGGTCAGGTTCATCGCGCAAGATTGAAGGGACAGGAGGTTGTTGTTAAAGTGCAAAGGCCTGGTCTCAAGGATCTTTTTGATATTGATCTAAAAAACCTTAGGGTTATTGCTGAATATCTTCAAAAAATTGATCCAAAGTCAGATGGTGCAAAGAGGGATTGGGTTGCTATTTATGATGAATGTGCCTCTGTCTTATATCAGGAGATCGATTACACTAAGGAGGCTGCTAATGCAGAATTGTTTGCTAGTAACTTTAAGAACATGAGTTATGTGAAAGTTCCTTCAATCTACTGGGATTATACCACGCCACAGATTCTGACAATGGAATATGTTCCAGGAATTAAAATAAACAAAATACAAGCTTTAGATCAACTTGGTGTTGATCGCGCAAGGTTAGGGAGATATGCAGTTGAATCGTTCTTGGAACAAATTTTATCGCATGGTTTCTTCCATGCTGACCCTCATCCTGGAAATATTGCAGTTGATGATGTCAATGGTGGAAGATTGATCTTCTACGATTTCGGAATGATGGGGAGCATCAGTCCAAACATCCGAGAAGGCTTGCTTGAGACATTTTATGGAGTCTATGAGAAGGATCCAGATAGGGTTCTTCAATCAATGATTCAAATGGGTGTTCTTGTTCCTACCGGAGATATGACTGCTGTTAGGCGAACAGCACAATTCTTTCTTAACAGTTTTGAGGAGCGCTTGGCTGCACAAAGGAAAGAGAAAGAGGAAGCAACAGGCGAACTTGGATTCAAAAAACCATTAAGCAAGGAGGAAAAAATAATGAAAAAGAAACAACGTCTAGCTGCTATTGGTGAAGATTTATTATCCATTTCATCAGACCAACCCTTCCGGTTTCCTGCTACATTTACATTTGTTGTTAGAGCTTTCTCAGTGTTGGATGGCATTGGAAAGGGCCTTGACCCACGTTTTGACATTACTGAGATTGCCAAACCTTATGCTCTAGAGTTACTAAGGTTTCGTGAGGCAGGGGTTGAAGTTGTCTTAAAGGATTTCACTAAGAGATGGGATAGACAATCTCAAGCTTTTTACAACTTATTTAGGCAGGCTGACAGGGTGGACAAGCTTGCTTCAATTATCCAAAGACTGGAGCAAGGAGATCTAAAGCTCCGAGTTCGAACTTTAGAATCTGAAAGGGCATTCCAGCGTGTTGCCACAGTCCAGAAGACTATAGGAAATGCAGTAGCTGCTGGAAGCCTAATAAATCTTGCGACAATTTTGTATCTCAATTCGATTCGCGTGCCTGCAATTGCAGCATATGTCTTCTGTGCATTCTTTGGTTTTCAAGTGCTCTTGGGCATTGTAAAGGTCAAGAAGCTAGATGAAACGGAGCGATTGATAACAGGAACTGCATAA |
| Protein sequence |
>Msa0473320 MATTSSLLHLPELHFLSPQITLRNRLSFSTRSISRFSTSNVTLRATRIRSSKDESAIAERVNDVEWSGNGVASRAGRNGAAGGYVNGAVNGSSVKYGNGNGVASTVVVDVEASKVNEDGRKRRLEEIGKEDAWFKQTEEQKVEVAVAPGGRWSRFKTYSTIQRTLEIWGFVVTFIFKAWLDNQKFSYKGGMTEEKKKLRRKTLAKWLKESILRLGPTFIKIGQQFSTRVDILPQEYVDQLSELQDQVPPFPSESALAIVEEELGASVGDIFDQFDYEPIAAASLGQVHRARLKGQEVVVKVQRPGLKDLFDIDLKNLRVIAEYLQKIDPKSDGAKRDWVAIYDECASVLYQEIDYTKEAANAELFASNFKNMSYVKVPSIYWDYTTPQILTMEYVPGIKINKIQALDQLGVDRARLGRYAVESFLEQILSHGFFHADPHPGNIAVDDVNGGRLIFYDFGMMGSISPNIREGLLETFYGVYEKDPDRVLQSMIQMGVLVPTGDMTAVRRTAQFFLNSFEERLAAQRKEKEEATGELGFKKPLSKEEKIMKKKQRLAAIGEDLLSISSDQPFRFPATFTFVVRAFSVLDGIGKGLDPRFDITEIAKPYALELLRFREAGVEVVLKDFTKRWDRQSQAFYNLFRQADRVDKLASIIQRLEQGDLKLRVRTLESERAFQRVATVQKTIGNAVAAGSLINLATILYLNSIRVPAIAAYVFCAFFGFQVLLGIVKVKKLDETERLITGTA* |
