Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452800 | A0A396GR42 | 97.619 | 336 | 2 | 1 | 1 | 330 | 1 | 336 | 0.0 | 668 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000180 | Msa1452800 | 0.831536 | 1.578835e-55 | -8.615850e-47 |
| Msa0000220 | Msa1452800 | 0.828174 | 1.040875e-54 | -8.615850e-47 |
| Msa0000610 | Msa1452800 | 0.877260 | 7.244541e-69 | -8.615850e-47 |
| Msa0000800 | Msa1452800 | -0.849473 | 3.162061e-60 | -8.615850e-47 |
| Msa0001040 | Msa1452800 | 0.829091 | 6.247896e-55 | -8.615850e-47 |
| Msa0001420 | Msa1452800 | 0.818275 | 2.133865e-52 | -8.615850e-47 |
| Msa0001510 | Msa1452800 | 0.915263 | 7.241168e-85 | -8.615850e-47 |
| Msa0001540 | Msa1452800 | 0.834403 | 3.058975e-56 | -8.615850e-47 |
| Msa0001550 | Msa1452800 | 0.814917 | 1.206574e-51 | -8.615850e-47 |
| Msa0002110 | Msa1452800 | 0.888767 | 4.406141e-73 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452800 | MtrunA17_Chr8g0383001 | 97.619 | 336 | 2 | 1 | 1 | 330 | 1 | 336 | 0.0 | 668 |
| Msa1452800 | MtrunA17_Chr2g0310591 | 35.088 | 171 | 99 | 2 | 166 | 327 | 218 | 385 | 1.82e-21 | 94.0 |
| Msa1452800 | MtrunA17_Chr7g0273361 | 28.931 | 159 | 100 | 3 | 182 | 329 | 178 | 334 | 5.94e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452800 | AT2G21960.1 | 83.039 | 283 | 48 | 0 | 48 | 330 | 50 | 332 | 2.73e-172 | 481 |
| Msa1452800 | AT1G56180.1 | 32.143 | 168 | 105 | 1 | 171 | 329 | 217 | 384 | 1.34e-19 | 89.0 |
| Msa1452800 | AT5G27290.1 | 31.447 | 159 | 96 | 4 | 182 | 329 | 179 | 335 | 6.63e-14 | 72.0 |
Find 88 sgRNAs with CRISPR-Local
Find 220 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCTGCTAATAGCTGCT+TGG | 0.552163 | tig0034162:+25629 | None:intergenic |
| AAATTGGTTGGGCCAAGAAA+TGG | 0.440413 | tig0034162:-27414 | Msa1452800:CDS |
| AACTGGATTCTTGGGTATTC+TGG | 0.263208 | tig0034162:-26660 | Msa1452800:CDS |
| AAGCTCTGGTTGTGAATTTA+GGG | 0.294762 | tig0034162:+27779 | None:intergenic |
| AATGGCATCGATGGCAAAGA+AGG | 0.499709 | tig0034162:-24590 | Msa1452800:CDS |
| AATTGTTCCAACCTCATTGG+AGG | 0.533072 | tig0034162:+27938 | None:intergenic |
| ACCGTACGACGTGAAACATA+TGG | 0.511639 | tig0034162:+27382 | None:intergenic |
| ACTCTAGCTCTAGAGATCAA+TGG | 0.513577 | tig0034162:+24344 | None:intergenic |
| AGAAGGTTTAACATATGACA+AGG | 0.674545 | tig0034162:-24994 | Msa1452800:CDS |
| AGACAGCAATGCAGTTAAAG+AGG | 0.489669 | tig0034162:-27455 | Msa1452800:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGTAATGGTCAATTACCT+TGG | + | tig0034162:24591-24610 | None:intergenic | 30.0% | |
| TGGATTGAATACAATTTCAC+GGG | + | tig0034162:24736-24755 | None:intergenic | 30.0% | |
| CAATCCATTGTTCATTGAAA+TGG | - | tig0034162:24749-24768 | Msa1452800:intron | 30.0% | |
| ACTACCATTTCAATGAACAA+TGG | + | tig0034162:24756-24775 | None:intergenic | 30.0% | |
| AAATGGTAGTTTATACCGTT+TGG | - | tig0034162:24766-24785 | Msa1452800:intron | 30.0% | |
| AATGGTAGTTTATACCGTTT+GGG | - | tig0034162:24767-24786 | Msa1452800:intron | 30.0% | |
| GTTCAGTTATATTACCCAAA+CGG | + | tig0034162:24784-24803 | None:intergenic | 30.0% | |
| CAATGTAACAATATGTGTTG+TGG | + | tig0034162:25068-25087 | None:intergenic | 30.0% | |
| AAATGTTAACAAGTGTCCTT+GGG | - | tig0034162:25190-25209 | Msa1452800:intron | 30.0% | |
| AATATTTAACTAGTGCCCTA+AGG | - | tig0034162:25302-25321 | Msa1452800:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0034162 | gene | 24306 | 28009 | 24306 | ID=Msa1452800;Name=Msa1452800 |
| tig0034162 | mRNA | 24306 | 28009 | 24306 | ID=Msa1452800-mRNA-1;Parent=Msa1452800;Name=Msa1452800-mRNA-1;_AED=0.07;_eAED=0.07;_QI=107|1|1|1|1|1|10|240|330 |
| tig0034162 | exon | 24306 | 24665 | 24306 | ID=Msa1452800-mRNA-1:exon:18814;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 24793 | 24858 | 24793 | ID=Msa1452800-mRNA-1:exon:18813;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 24959 | 25049 | 24959 | ID=Msa1452800-mRNA-1:exon:18812;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 25317 | 25446 | 25317 | ID=Msa1452800-mRNA-1:exon:18811;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 25569 | 25657 | 25569 | ID=Msa1452800-mRNA-1:exon:18810;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 25743 | 25819 | 25743 | ID=Msa1452800-mRNA-1:exon:18809;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 26643 | 26711 | 26643 | ID=Msa1452800-mRNA-1:exon:18808;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 26835 | 26909 | 26835 | ID=Msa1452800-mRNA-1:exon:18807;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 27384 | 27479 | 27384 | ID=Msa1452800-mRNA-1:exon:18806;Parent=Msa1452800-mRNA-1 |
| tig0034162 | exon | 27723 | 28009 | 27723 | ID=Msa1452800-mRNA-1:exon:18805;Parent=Msa1452800-mRNA-1 |
| tig0034162 | five_prime_UTR | 27903 | 28009 | 27903 | ID=Msa1452800-mRNA-1:five_prime_utr;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 27723 | 27902 | 27723 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 27384 | 27479 | 27384 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 26835 | 26909 | 26835 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 26643 | 26711 | 26643 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 25743 | 25819 | 25743 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 25569 | 25657 | 25569 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 25317 | 25446 | 25317 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 24959 | 25049 | 24959 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 24793 | 24858 | 24793 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | CDS | 24546 | 24665 | 24546 | ID=Msa1452800-mRNA-1:cds;Parent=Msa1452800-mRNA-1 |
| tig0034162 | three_prime_UTR | 24306 | 24545 | 24306 | ID=Msa1452800-mRNA-1:three_prime_utr;Parent=Msa1452800-mRNA-1 |
| Gene Sequence |
>Msa1452800 ATGTTGATCACAACCATGCCTTACTCATCATCTTTACCCAAACCATATTATAATTTTTACCATAATCATCATCACCACCAAACATGCTTCAACATCAACCTCCCTAAATTCACAACCAGAGCTTCTTCCTCTGCTGCACCTGGTGTCGACCTCAACACTCTTGATTCTGCCATTGCCAAGAAAGACAGCAATGCAGTTAAAGAGGCACTTGATCAACTGAGTGAAATTGGTTGGGCCAAGAAATGGAGTTCTCAGCCATATGTTTCACGTCGTACGACATCACTTCGAGAGCTGACAACTTTAGGGATTAAAAATGCGGAGAACCTTGCAATTCCTAGTGTCAGAAATGATGCAGCTTTTCTCTTTACTGTGGTTGGAACAACTGGATTCTTGGGTATTCTGGCTGGCCAGCTTCCCGGGGATTGGGGCTTCTTTGTACCATACTTGATTGGGAGTATTTCCTTAGTGGTATTGGGTATAGGCAGCACATCTCCTGGGCTTCTCCAAGCAGCTATTAGCAGCTTTTCAGTTGTTTTTCCTGATTATCAAGAAAGGATTGCTAGACATGAAGCAGCCCACTTTTTGATTGCTTATCTGCTTGGCTTACCCATTTTGGGATATTCACTGGATATTGGAAAAGAGCATGTCAATCTCATTGATGATAGGCTTGAAAAGCTTCTCTATAGTGGTCAGCTTGATGCTAAGGAGATAGATAGATTGGCTGTTGTATCAATGGCTGGACTGGCAGCAGAAGGTTTAACATATGACAAGGTTGTTGGTCAATCTGCTGATCTTTTCACTCTCCAGAGGTTTATAAACAGAACCAAACCACAACTCAGCAAAGATCAGCAACAAAATCTCACTAGATGGGCAGTTTTGTTCGCTGCTTCTCTATTGAAAAACAACAAGTCGATCCATGAAGCCTTAATGGCATCGATGGCAAAGAAGGCAAGTGTACTCGAGTGCATTCAAACAATCGAAAGTGCATCATAG |
| Protein sequence |
>Msa1452800 MLITTMPYSSSLPKPYYNFYHNHHHHQTCFNINLPKFTTRASSSAAPGVDLNTLDSAIAKKDSNAVKEALDQLSEIGWAKKWSSQPYVSRRTTSLRELTTLGIKNAENLAIPSVRNDAAFLFTVVGTTGFLGILAGQLPGDWGFFVPYLIGSISLVVLGIGSTSPGLLQAAISSFSVVFPDYQERIARHEAAHFLIAYLLGLPILGYSLDIGKEHVNLIDDRLEKLLYSGQLDAKEIDRLAVVSMAGLAAEGLTYDKVVGQSADLFTLQRFINRTKPQLSKDQQQNLTRWAVLFAASLLKNNKSIHEALMASMAKKASVLECIQTIESAS* |
