AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25666


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25666.t1 MTR_7g107680 86.813 182 19 2 1 177 1 182 2.76e-100 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25666.t1 AT4G03150 60.000 130 50 1 44 171 52 181 5.78e-52 164

Find 0 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTTTCCTTATAAGAAA+TGG - 51708:5480-5499 None:intergenic 20.0%
!!! TTTTTCCATTCACAATTATT+AGG + 51708:5283-5302 MS.gene25666:intron 20.0%
! ATTGGAAACCATTTCTTATA+AGG + 51708:5469-5488 MS.gene25666:CDS 25.0%
! GAATTGGTTACATATTGTTA+AGG + 51708:5045-5064 MS.gene25666:intron 25.0%
! TGATTCCTAATAATTGTGAA+TGG - 51708:5291-5310 None:intergenic 25.0%
!! CTTATAAGGAAAGTTTTCAT+GGG + 51708:5483-5502 MS.gene25666:CDS 25.0%
!! TCTTATAAGGAAAGTTTTCA+TGG + 51708:5482-5501 MS.gene25666:CDS 25.0%
!! TTAGGGTTACTTTACATAAA+TGG + 51708:5318-5337 MS.gene25666:CDS 25.0%
!! TTATAAGGAAAGTTTTCATG+GGG + 51708:5484-5503 MS.gene25666:CDS 25.0%
AAATGGATGAGAATGTGAAT+GGG - 51708:4874-4893 None:intergenic 30.0%
ACAATTATTAGGAATCAGAG+TGG + 51708:5294-5313 MS.gene25666:intron 30.0%
CAAAGAATTGTAGTAGGATT+TGG - 51708:5399-5418 None:intergenic 30.0%
CCTACTACAATTCTTTGATA+CGG + 51708:5402-5421 MS.gene25666:CDS 30.0%
TGAGATGTTCATGTTAGAAT+TGG + 51708:5029-5048 MS.gene25666:intron 30.0%
! AGCTGACATTTTGTTGAATA+TGG + 51708:5439-5458 MS.gene25666:CDS 30.0%
! GGTTACTTTACATAAATGGT+TGG + 51708:5322-5341 MS.gene25666:CDS 30.0%
!! ACGTGTTTCTAAAATTTGAG+AGG - 51708:4994-5013 None:intergenic 30.0%
ATGAAGATATAAATGAGCGC+AGG - 51708:5253-5272 None:intergenic 35.0%
CCGTATCAAAGAATTGTAGT+AGG - 51708:5405-5424 None:intergenic 35.0%
GAAATGGATGAGAATGTGAA+TGG - 51708:4875-4894 None:intergenic 35.0%
! AAGATGAAGAAGAAGCGTAT+TGG - 51708:4818-4837 None:intergenic 35.0%
!! AATGAAAAGAGTACCTACCT+GGG - 51708:4967-4986 None:intergenic 35.0%
AAAGTAATCACATGTGTCCG+TGG + 51708:5174-5193 MS.gene25666:intron 40.0%
ATCTCCACATTTGTTTCCTC+AGG - 51708:5362-5381 None:intergenic 40.0%
ATTAGGAATCAGAGTGGCTT+AGG + 51708:5300-5319 MS.gene25666:intron 40.0%
CTAGTGATTTCAACACCGAA+AGG - 51708:5200-5219 None:intergenic 40.0%
GTGAGTGAATGTTACTGTCA+CGG - 51708:4793-4812 None:intergenic 40.0%
GTTGAAATCACTAGGTGGTT+AGG + 51708:5206-5225 MS.gene25666:intron 40.0%
GTTGAATATGGCACGTGAAT+TGG + 51708:5451-5470 MS.gene25666:CDS 40.0%
TTAGGAATCAGAGTGGCTTA+GGG + 51708:5301-5320 MS.gene25666:intron 40.0%
! ATGAAGAAGAAGCGTATTGG+TGG - 51708:4815-4834 None:intergenic 40.0%
! CGAGTAGTGTGGACAATTTT+TGG - 51708:4849-4868 None:intergenic 40.0%
!! CTTTCGGTGTTGAAATCACT+AGG + 51708:5198-5217 MS.gene25666:intron 40.0%
!! GAATGAAAAGAGTACCTACC+TGG - 51708:4968-4987 None:intergenic 40.0%
!!! GTGGACAATTTTTGGTGAAG+AGG - 51708:4841-4860 None:intergenic 40.0%
AGGAGGAAGATGGTGTGAAT+GGG - 51708:4770-4789 None:intergenic 45.0%
AGTGAATGTTACTGTCACGG+AGG - 51708:4790-4809 None:intergenic 45.0%
ATTTCAACACCGAAAGGCCA+CGG - 51708:5194-5213 None:intergenic 45.0%
CTGTCCTGAGGAAACAAATG+TGG + 51708:5355-5374 MS.gene25666:CDS 45.0%
GAACACGAAGGTCTTCAACT+TGG - 51708:4914-4933 None:intergenic 45.0%
GGATGATGAATACTGTCCTG+AGG + 51708:5343-5362 MS.gene25666:CDS 45.0%
GTCTTCAACTTGGTCGTCAT+CGG - 51708:4904-4923 None:intergenic 45.0%
TCTTCAACTTGGTCGTCATC+GGG - 51708:4903-4922 None:intergenic 45.0%
TGGTTGGGAGTAACCAATGA+GGG - 51708:4938-4957 None:intergenic 45.0%
TTGGTTGGGAGTAACCAATG+AGG - 51708:4939-4958 None:intergenic 45.0%
! TAGTTAGCTTAGACCGACGA+AGG + 51708:5110-5129 MS.gene25666:intron 45.0%
!! TCGGTGTTGAAATCACTAGG+TGG + 51708:5201-5220 MS.gene25666:intron 45.0%
AGCCGCATTTGCAGATGTGT+AGG - 51708:5510-5529 None:intergenic 50.0%
CTGTAAACCTAATCGCGCAG+CGG + 51708:5528-5547 MS.gene25666:CDS 50.0%
GAATGTTACTGTCACGGAGG+AGG - 51708:4787-4806 None:intergenic 50.0%
GAGGAGGAAGATGGTGTGAA+TGG - 51708:4771-4790 None:intergenic 50.0%
GGCCTACACATCTGCAAATG+CGG + 51708:5505-5524 MS.gene25666:CDS 50.0%
GGTTGGGAGTAACCAATGAG+GGG - 51708:4937-4956 None:intergenic 50.0%
GTGAATGGGTACGAGTAGTG+TGG - 51708:4860-4879 None:intergenic 50.0%
GTGTGAATGGGATGATGCTG+CGG - 51708:4758-4777 None:intergenic 50.0%
GTTGGGAGTAACCAATGAGG+GGG - 51708:4936-4955 None:intergenic 50.0%
TGTGAATGGGATGATGCTGC+GGG - 51708:4757-4776 None:intergenic 50.0%
!! GAAAAGAGTACCTACCTGGG+AGG - 51708:4964-4983 None:intergenic 50.0%
!! TCGTCATCGGGTTCTGGAAA+TGG - 51708:4891-4910 None:intergenic 50.0%
ACCAATGAGGGGGAACACGA+AGG - 51708:4926-4945 None:intergenic 55.0%
ACCTTCGTGTTCCCCCTCAT+TGG + 51708:4922-4941 MS.gene25666:CDS 55.0%
ACTGTCACGGAGGAGGAAGA+TGG - 51708:4780-4799 None:intergenic 55.0%
ACTTGGTCGTCATCGGGTTC+TGG - 51708:4897-4916 None:intergenic 55.0%
CACATGTGTCCGTGGCCTTT+CGG + 51708:5182-5201 MS.gene25666:intron 55.0%
CGACGAAGGACACGTCAACA+CGG + 51708:5124-5143 MS.gene25666:intron 55.0%
GCTGCGGGGAGATAGAATGA+TGG - 51708:4742-4761 None:intergenic 55.0%
GTGAATGGGATGATGCTGCG+GGG - 51708:4756-4775 None:intergenic 55.0%
GTTCAATCCGCTGCGCGATT+AGG - 51708:5538-5557 None:intergenic 55.0%
! AGTACCTACCTGGGAGGCAT+TGG - 51708:4958-4977 None:intergenic 55.0%
! CGTGTTGACGTGTCCTTCGT+CGG - 51708:5126-5145 None:intergenic 55.0%
!! CTACCTGGGAGGCATTGGTT+GGG - 51708:4953-4972 None:intergenic 55.0%
ACTCCCAACCAATGCCTCCC+AGG + 51708:4947-4966 MS.gene25666:CDS 60.0%
CCAACCAATGCCTCCCAGGT+AGG + 51708:4951-4970 MS.gene25666:intron 60.0%
!! CCTACCTGGGAGGCATTGGT+TGG - 51708:4954-4973 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
51708 gene 4708 5577 4708 ID=MS.gene25666
51708 mRNA 4708 5577 4708 ID=MS.gene25666.t1;Parent=MS.gene25666
51708 exon 4708 4968 4708 ID=MS.gene25666.t1.exon1;Parent=MS.gene25666.t1
51708 CDS 4708 4968 4708 ID=cds.MS.gene25666.t1;Parent=MS.gene25666.t1
51708 exon 5305 5577 5305 ID=MS.gene25666.t1.exon2;Parent=MS.gene25666.t1
51708 CDS 5305 5577 5305 ID=cds.MS.gene25666.t1;Parent=MS.gene25666.t1
Gene Sequence

>MS.gene25666

ATGTCAAGCTTGAAATTGAATCTAGTTCATACCATCATTCTATCTCCCCGCAGCATCATCCCATTCACACCATCTTCCTCCTCCGTGACAGTAACATTCACTCACCACCAATACGCTTCTTCTTCATCTTCCTCTTCACCAAAAATTGTCCACACTACTCGTACCCATTCACATTCTCATCCATTTCCAGAACCCGATGACGACCAAGTTGAAGACCTTCGTGTTCCCCCTCATTGGTTACTCCCAACCAATGCCTCCCAGGTAGGTACTCTTTTCATTCATTCCTCTCAAATTTTAGAAACACGTAGTTGTACTTGTAGCTGAGATGTTCATGTTAGAATTGGTTACATATTGTTAAGGTAGTTACAATTGAGTTATGATTAGTTAAAAGTTAGTTAGAATTAGTTAGCTTAGACCGACGAAGGACACGTCAACACGGTAATAATTTGAGAAAACAGCATTGTTGAAAGTAATCACATGTGTCCGTGGCCTTTCGGTGTTGAAATCACTAGGTGGTTAGGATCACTATAAAAAAAATGTATCCTGCGCTCATTTATATCTTCATTAATTGTTTTTTTTTCCATTCACAATTATTAGGAATCAGAGTGGCTTAGGGTTACTTTACATAAATGGTTGGATGATGAATACTGTCCTGAGGAAACAAATGTGGAGATAAGCAGAGTTGCTGCCAAATCCTACTACAATTCTTTGATACGGAAACAAGCTGATTTAGCTGACATTTTGTTGAATATGGCACGTGAATTGGAAACCATTTCTTATAAGGAAAGTTTTCATGGGGCCTACACATCTGCAAATGCGGCTGTAAACCTAATCGCGCAGCGGATTGAACAATTCTGCCACCCAAATTGA

Protein sequence

>MS.gene25666.t1

MSSLKLNLVHTIILSPRSIIPFTPSSSSVTVTFTHHQYASSSSSSSPKIVHTTRTHSHSHPFPEPDDDQVEDLRVPPHWLLPTNASQESEWLRVTLHKWLDDEYCPEETNVEISRVAAKSYYNSLIRKQADLADILLNMARELETISYKESFHGAYTSANAAVNLIAQRIEQFCHPN