AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74217

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74217.t1 MTR_5g059410 98.241 739 8 1 3 741 2 735 0.0 1501
MS.gene74217.t1 MTR_1g116120 98.241 739 8 1 3 741 2 735 0.0 1501
MS.gene74217.t1 MTR_4g024630 82.970 734 117 2 7 740 9 734 0.0 1259
MS.gene74217.t1 MTR_4g024630 88.235 578 68 0 163 740 3 580 0.0 1073
MS.gene74217.t1 MTR_0776s0010 51.874 667 306 9 74 731 921 1581 0.0 639
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74217.t1 AT2G45290 81.016 748 126 2 1 740 1 740 0.0 1276
MS.gene74217.t1 AT2G45290 81.016 748 126 2 1 740 1 740 0.0 1276
MS.gene74217.t1 AT3G60750 82.927 738 126 0 1 738 1 738 0.0 1273
MS.gene74217.t1 AT3G60750 82.791 738 126 1 1 738 1 737 0.0 1266

Find 214 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAGCCTTTGCTTCCTTAA+TGG 0.175474 1.2:-86206326 None:intergenic
AGTGATGATGCACGAATTTC+AGG 0.178899 1.2:-86204781 None:intergenic
AAGGTTCTTCCTGGTCTTAT+TGG 0.215510 1.2:+86207215 MS.gene74217:CDS
AATCGAACATTGCGCTCTTC+TGG 0.242416 1.2:-86207308 None:intergenic
TTAAACCAAGAAGGATTCTT+TGG 0.258486 1.2:-86205093 None:intergenic
AGACAAACCTACTTTGATTA+AGG 0.266496 1.2:+86206351 MS.gene74217:CDS
TATGCTCTTCTTCATCTTGC+TGG 0.269634 1.2:+86205162 MS.gene74217:CDS
GTAGCATCAGCAGGGGTTTC+CGG 0.275225 1.2:-86207155 None:intergenic
ACCATTGGCATTGATCGATT+TGG 0.287349 1.2:+86207998 MS.gene74217:CDS
CTTGCCGGACATTGGGGTCT+TGG 0.291105 1.2:+86206148 MS.gene74217:CDS
CACTTGGATGGCATGTGATT+TGG 0.296401 1.2:+86206263 MS.gene74217:CDS
GCAAAAGTTGCCTAACCTTC+CGG 0.305640 1.2:+86207673 MS.gene74217:CDS
CCTCAATTCTTGCCCTTTCT+CGG 0.314282 1.2:+86207651 MS.gene74217:CDS
ATAACCCAAAGAATCCTTCT+TGG 0.318239 1.2:+86205088 MS.gene74217:CDS
GCTCTTCTGGAGTAGCCTTT+TGG 0.321780 1.2:-86207295 None:intergenic
ATGACTTTGTTGAAATCGTT+TGG 0.325235 1.2:+86207266 MS.gene74217:CDS
TGCTGTTGAGAAAGCTAATT+CGG 0.326611 1.2:+86204999 MS.gene74217:CDS
GTGTTCACTTGCCGGACATT+GGG 0.326670 1.2:+86206141 MS.gene74217:CDS
ACAGATCTATGAAACTTCTT+TGG 0.327073 1.2:-86208089 None:intergenic
CTGCCGCCGCTGATGATCTA+AGG 0.331163 1.2:+86207807 MS.gene74217:CDS
AATGAAGCGTGTTCACTTGC+CGG 0.335597 1.2:+86206133 MS.gene74217:CDS
TGAAATTCGTGCAGCCATTA+AGG 0.339511 1.2:+86206312 MS.gene74217:CDS
TTGTTTAGGTCCTCTGGGTC+AGG 0.341996 1.2:+86205853 MS.gene74217:intron
GCTTCGAGTGCAGCACCCTC+AGG 0.351157 1.2:-86206830 None:intergenic
CTGTAGGAATTAGCTTTGTT+TGG 0.355528 1.2:-86206607 None:intergenic
GTTACCACTACCATTGGATT+TGG 0.357397 1.2:+86206580 MS.gene74217:CDS
GCCCTGGCCAAGGTTCTTCC+TGG 0.357791 1.2:+86207206 MS.gene74217:CDS
AATTTCAGGGTTGAGGTATA+CGG 0.371548 1.2:-86204767 None:intergenic
CAAGAATTGAGGGTCTCTTC+TGG 0.372757 1.2:-86207640 None:intergenic
CAAAAGTTGCCTAACCTTCC+GGG 0.374567 1.2:+86207674 MS.gene74217:CDS
TGCTCTAGGTATGTTATATT+AGG 0.377773 1.2:+86206082 MS.gene74217:intron
GCAATGTTCGATTTGGTGTC+AGG 0.378142 1.2:+86207318 MS.gene74217:CDS
AAGCTTGCCAGGTGCTCTAT+TGG 0.380074 1.2:-86207536 None:intergenic
ACCCATGGGTTGTGCTCCAA+TGG 0.386484 1.2:+86205035 MS.gene74217:CDS
GCATTGCTCGGAAGCTTGCC+AGG 0.387618 1.2:-86207547 None:intergenic
TTCTATTGAAGGTGTTGAAA+AGG 0.389406 1.2:+86207700 MS.gene74217:CDS
ACAAAGAAAGTTGCACAGTA+TGG 0.395938 1.2:-86207398 None:intergenic
TCGTGAATTACCTGCTGATT+GGG 0.402483 1.2:+86206928 MS.gene74217:CDS
GGCTGGTGTGGTTCTGCGAC+GGG 0.402529 1.2:-86204884 None:intergenic
ATTGAGGCGGGATCAACATT+TGG 0.403681 1.2:+86207944 MS.gene74217:CDS
CCGTGCAGCCAAGTGCTTCT+CGG 0.404020 1.2:-86205906 None:intergenic
GAAGAGCGCAATGTTCGATT+TGG 0.405488 1.2:+86207311 MS.gene74217:CDS
ACAGCGGTGGCGCGGATGGC+TGG 0.406479 1.2:-86204901 None:intergenic
ACTTGGATGGCATGTGATTT+GGG 0.407932 1.2:+86206264 MS.gene74217:CDS
ATCGTGAATTACCTGCTGAT+TGG 0.408577 1.2:+86206927 MS.gene74217:CDS
CCGGCTGAGGAGGTTGCTGC+TGG 0.411767 1.2:-86204859 None:intergenic
GGGAAAATATACAAGGAGTA+TGG 0.412527 1.2:+86208037 MS.gene74217:CDS
CGTGTTCACTTGCCGGACAT+TGG 0.412603 1.2:+86206140 MS.gene74217:CDS
TGTGGTTCTGCGACGGGAAC+CGG 0.416883 1.2:-86204878 None:intergenic
CAGGGTATTGCCAATGGTGT+TGG 0.421847 1.2:+86205872 MS.gene74217:CDS
CAGTGTTCACGGAGCTGCAT+TGG 0.425068 1.2:+86206627 MS.gene74217:CDS
GTTATGACCCATGATTCAAT+TGG 0.427272 1.2:+86207485 MS.gene74217:CDS
TAAACCAAGAAGGATTCTTT+GGG 0.427395 1.2:-86205092 None:intergenic
CTTGAAGCAGTTTCGACAAT+GGG 0.430255 1.2:+86205663 MS.gene74217:CDS
GAAGGAGAGGGAAGAAGTGG+AGG 0.433182 1.2:-86204806 None:intergenic
CCCGAGAACTTCGAGACATA+TGG 0.433908 1.2:+86205706 MS.gene74217:CDS
TTTGGTTGGCATAAGATTGT+TGG 0.433955 1.2:+86207962 MS.gene74217:CDS
TGATTGGGAGAAAGCACTTC+CGG 0.439162 1.2:+86206943 MS.gene74217:CDS
AGCAGAAATAACAGCTTCCT+TGG 0.441956 1.2:-86208065 None:intergenic
ATTAGGAGATGGTTGTCAAA+TGG 0.442289 1.2:+86206099 MS.gene74217:CDS
GGACGAAGCATCAAAACATT+TGG 0.442772 1.2:-86207569 None:intergenic
CTCCAAGTCCAATTGAATCA+TGG 0.443401 1.2:-86207493 None:intergenic
AGTGTTCACGGAGCTGCATT+GGG 0.447236 1.2:+86206628 MS.gene74217:CDS
ATAGAAGTTCCCGGAAGGTT+AGG 0.449217 1.2:-86207683 None:intergenic
ACTCTAGCTGTCACAGAAGC+AGG 0.449310 1.2:-86207917 None:intergenic
GGCACATGGAAAGGCTCATA+TGG 0.449701 1.2:-86206691 None:intergenic
GACCAGGAAGAACCTTGGCC+AGG 0.451334 1.2:-86207208 None:intergenic
ACACAAGGCAGAAATCCTTA+TGG 0.451743 1.2:-86207444 None:intergenic
CCGAGAAAGGGCAAGAATTG+AGG 0.453215 1.2:-86207651 None:intergenic
AGATTTCTTGTAGCATCAGC+AGG 0.453550 1.2:-86207164 None:intergenic
GGAGGATTTGAGTGCAGGGA+AGG 0.456187 1.2:-86204824 None:intergenic
CCCATGGGTTGTGCTCCAAT+GGG 0.456524 1.2:+86205036 MS.gene74217:CDS
ACCCATGATTCAATTGGACT+TGG 0.457470 1.2:+86207491 MS.gene74217:CDS
TGCTCTATTGGCTGATGAGT+TGG 0.458639 1.2:-86207524 None:intergenic
TCTGCGACGGGAACCGGCTG+AGG 0.459678 1.2:-86204872 None:intergenic
TTCGACAGCGGTGGCGCGGA+TGG 0.460246 1.2:-86204905 None:intergenic
ACACCTTCAATAGAAGTTCC+CGG 0.462136 1.2:-86207692 None:intergenic
GATGCTACAAGGCAGAATCT+TGG 0.465252 1.2:+86206664 MS.gene74217:CDS
CTTCCGGGAACTTCTATTGA+AGG 0.465653 1.2:+86207689 MS.gene74217:CDS
CACAGTATGGAATGAATCCC+GGG 0.466716 1.2:-86207385 None:intergenic
GGGTCACCCGGGTTTACCCA+TGG 0.467220 1.2:+86205020 MS.gene74217:CDS
TCCATATGTCTCGAAGTTCT+CGG 0.472304 1.2:-86205707 None:intergenic
ACCTGATGAGTTGTCTGAAA+TGG 0.472367 1.2:-86207732 None:intergenic
TGGGTAAAGAACGGAAATAC+TGG 0.473280 1.2:+86206283 MS.gene74217:CDS
GATAATCACATATCTATTGA+TGG 0.473309 1.2:+86206193 MS.gene74217:CDS
GTTCTTCCTGGTCTTATTGG+TGG 0.473340 1.2:+86207218 MS.gene74217:CDS
CAACAAAGTCATGTTGGAAG+AGG 0.473739 1.2:-86207255 None:intergenic
TAGATCATCAGCGGCGGCAG+CGG 0.475603 1.2:-86207804 None:intergenic
TTTGAGTGCAGGGAAGGAGA+GGG 0.475826 1.2:-86204818 None:intergenic
GGTCACCCGGGTTTACCCAT+GGG 0.476254 1.2:+86205021 MS.gene74217:CDS
GCAGTTTCATTACCATCAGC+TGG 0.477799 1.2:-86207590 None:intergenic
TCTATTGAAGGTGTTGAAAA+GGG 0.479009 1.2:+86207701 MS.gene74217:CDS
TTTCTCAACGAGAGAAGCTT+CGG 0.481299 1.2:-86204944 None:intergenic
CGAGAAAGGGCAAGAATTGA+GGG 0.481642 1.2:-86207650 None:intergenic
GAAGAGGAGGATTTGAGTGC+AGG 0.481728 1.2:-86204829 None:intergenic
CCGAGAAGCACTTGGCTGCA+CGG 0.482213 1.2:+86205906 MS.gene74217:CDS
AGGGAAGGAGAGGGAAGAAG+TGG 0.484686 1.2:-86204809 None:intergenic
TCAATTGGACTTGGAGAGGA+TGG 0.485462 1.2:+86207500 MS.gene74217:CDS
GCTAATTCCTACAGTGTTCA+CGG 0.486135 1.2:+86206616 MS.gene74217:CDS
GTGCAGCACCCTCAGGGGTA+TGG 0.486259 1.2:-86206823 None:intergenic
ATTTGAGTGCAGGGAAGGAG+AGG 0.487969 1.2:-86204819 None:intergenic
ATTGGTTATCTCACGTGTAA+TGG 0.488265 1.2:-86205958 None:intergenic
ATTTCTGCCTTGTGTGAAGC+CGG 0.489758 1.2:+86207452 MS.gene74217:CDS
AGAGGAGGCTGCAGAGCTAA+AGG 0.490701 1.2:+86206895 MS.gene74217:CDS
GAAAGCTAATTCGGGTCACC+CGG 0.492182 1.2:+86205008 MS.gene74217:CDS
CCATATGTCTCGAAGTTCTC+GGG 0.492717 1.2:-86205706 None:intergenic
GCTGTTGAGAAAGCTAATTC+GGG 0.492728 1.2:+86205000 MS.gene74217:CDS
TCGAAGTTCTCGGGATGTCC+AGG 0.493290 1.2:-86205697 None:intergenic
TCACTAACCTTAATCAAAGT+AGG 0.493636 1.2:-86206358 None:intergenic
TTTCGTTGTTTAGGTCCTCT+GGG 0.495503 1.2:+86205848 MS.gene74217:intron
TGACAGGTTACCACTACCAT+TGG 0.495504 1.2:+86206574 MS.gene74217:intron
TGGTATTGCTCTTCATAGCC+CGG 0.502685 1.2:+86207367 MS.gene74217:CDS
GTGATGATGCACGAATTTCA+GGG 0.503452 1.2:-86204780 None:intergenic
AAGAGGAGGATTTGAGTGCA+GGG 0.503870 1.2:-86204828 None:intergenic
TGTTTAGGTCCTCTGGGTCA+GGG 0.504143 1.2:+86205854 MS.gene74217:intron
TGGTCAACAATCTCACTGTC+AGG 0.504469 1.2:-86205938 None:intergenic
ACATCTTCTGGCACATGGAA+AGG 0.505808 1.2:-86206700 None:intergenic
GTTCCAATCAAAATAACATC+AGG 0.510531 1.2:-86207761 None:intergenic
TCCAAGTCCAATTGAATCAT+GGG 0.511215 1.2:-86207492 None:intergenic
GGCACTTGCCGAGAAGCACT+TGG 0.512393 1.2:+86205898 MS.gene74217:CDS
CTTTCCAAGACCCCAATGTC+CGG 0.512394 1.2:-86206152 None:intergenic
CATAAGATTGTTGGAAGCAA+GGG 0.513540 1.2:+86207971 MS.gene74217:CDS
ACCATTTCAGACAACTCATC+AGG 0.514190 1.2:+86207731 MS.gene74217:CDS
GATTGGAACTGGTTCTGAGT+TGG 0.514884 1.2:+86207775 MS.gene74217:CDS
AGGCGGGATCAACATTTGGT+TGG 0.515586 1.2:+86207948 MS.gene74217:CDS
TTTGGAGCAAGTGCTCCAGC+AGG 0.522166 1.2:+86208016 MS.gene74217:CDS
TCAAAACATTTGGCATTGCT+CGG 0.524118 1.2:-86207559 None:intergenic
CGATTTCAACAAAGTCATGT+TGG 0.524839 1.2:-86207261 None:intergenic
CTGGGTCAGGGTATTGCCAA+TGG 0.525385 1.2:+86205866 MS.gene74217:CDS
TGGTGTCAGGGAACACGCAA+TGG 0.526615 1.2:+86207331 MS.gene74217:CDS
TGGCTGGTGTGGTTCTGCGA+CGG 0.526744 1.2:-86204885 None:intergenic
ATCGTTTGGAGATTTCCAAA+AGG 0.533264 1.2:+86207280 MS.gene74217:CDS
GCGACGGGAACCGGCTGAGG+AGG 0.535292 1.2:-86204869 None:intergenic
CTTCGAGTGCAGCACCCTCA+GGG 0.535328 1.2:-86206829 None:intergenic
GGTATTGCTCTTCATAGCCC+GGG 0.537364 1.2:+86207368 MS.gene74217:CDS
GCACAGTATGGAATGAATCC+CGG 0.542998 1.2:-86207386 None:intergenic
ATGCACGAATTTCAGGGTTG+AGG 0.545728 1.2:-86204774 None:intergenic
ACTACATGAGAGCTGCCATA+AGG 0.547176 1.2:+86207429 MS.gene74217:CDS
GCACAACCCATGGGTAAACC+CGG 0.548154 1.2:-86205027 None:intergenic
GCATAAGATTGTTGGAAGCA+AGG 0.550996 1.2:+86207970 MS.gene74217:CDS
TTCTTTCCTTAGATCATCAG+CGG 0.551042 1.2:-86207813 None:intergenic
TATTGCCAATGGTGTTGGAT+TGG 0.553474 1.2:+86205877 MS.gene74217:CDS
ACCCATTGGAGCACAACCCA+TGG 0.554735 1.2:-86205037 None:intergenic
CAATGGGGAAGCAAAACTCC+TGG 0.558532 1.2:+86205679 MS.gene74217:CDS
ACCAGGAAGAACCTTGGCCA+GGG 0.559298 1.2:-86207207 None:intergenic
GGTGTCAGGGAACACGCAAT+GGG 0.559362 1.2:+86207332 MS.gene74217:CDS
CCAGCAGCAACCTCCTCAGC+CGG 0.562774 1.2:+86204859 MS.gene74217:CDS
TTGATGCTTCGTCCAGCTGA+TGG 0.563709 1.2:+86207578 MS.gene74217:CDS
CTAAAGAAGTAGATGCTACA+AGG 0.563711 1.2:+86206653 MS.gene74217:CDS
AGTGCTTTCTCCCAATCAGC+AGG 0.564870 1.2:-86206938 None:intergenic
GCTAGAGTCAGCATTGAGGC+GGG 0.565493 1.2:+86207932 MS.gene74217:CDS
GCTGATGATCTAAGGAAAGA+AGG 0.567319 1.2:+86207815 MS.gene74217:CDS
CGATCACGATTAAACCAAGA+AGG 0.567796 1.2:-86205102 None:intergenic
CTCAACGAGAGAAGCTTCGG+TGG 0.568704 1.2:-86204941 None:intergenic
AGGTATGTTATATTAGGAGA+TGG 0.568803 1.2:+86206088 MS.gene74217:intron
AAATCTAAATGCCCTGGCCA+AGG 0.569724 1.2:+86207196 MS.gene74217:CDS
TCGTATAGAATGTGACCCAT+TGG 0.570278 1.2:-86205051 None:intergenic
ACAGCAAAATCTAAATGCCC+TGG 0.570304 1.2:+86207190 MS.gene74217:CDS
AGAACCAAATCCAATGGTAG+TGG 0.571113 1.2:-86206584 None:intergenic
TGATCAGTCAGATGAATACA+AGG 0.572341 1.2:+86207883 MS.gene74217:CDS
TCATCAGCCAATAGAGCACC+TGG 0.576120 1.2:+86207529 MS.gene74217:CDS
CAATGTTCGATTTGGTGTCA+GGG 0.577817 1.2:+86207319 MS.gene74217:CDS
GTGTTTCGACAGCGGTGGCG+CGG 0.578804 1.2:-86204909 None:intergenic
TGCTGGTTTGCATGAAGAGG+AGG 0.579652 1.2:-86204842 None:intergenic
TCTCAACAGCATCGATAGCA+AGG 0.579895 1.2:-86204987 None:intergenic
GGAGATGGTTGTCAAATGGA+AGG 0.581318 1.2:+86206103 MS.gene74217:CDS
GCTGATGGTAATGAAACTGC+TGG 0.585057 1.2:+86207593 MS.gene74217:CDS
GTTTGGAGAACCAAATCCAA+TGG 0.585421 1.2:-86206590 None:intergenic
TGCTGCTGGTTTGCATGAAG+AGG 0.585541 1.2:-86204845 None:intergenic
TGGAGTCGCCATACCCCTGA+GGG 0.588668 1.2:+86206815 MS.gene74217:CDS
AGCTAGAGTCAGCATTGAGG+CGG 0.588951 1.2:+86207931 MS.gene74217:CDS
ACTTGAAGCAGTTTCGACAA+TGG 0.594500 1.2:+86205662 MS.gene74217:CDS
GGTGGCGCGGATGGCTGGTG+TGG 0.595740 1.2:-86204896 None:intergenic
CTCTAGCTGTCACAGAAGCA+GGG 0.598577 1.2:-86207916 None:intergenic
GGTCATAACATAAATAACTC+CGG 0.599500 1.2:-86207471 None:intergenic
TGCAGCCATTAAGGAAGCAA+AGG 0.600893 1.2:+86206321 MS.gene74217:CDS
TGATTCAATTGGACTTGGAG+AGG 0.602455 1.2:+86207496 MS.gene74217:CDS
TGTTCACTTGCCGGACATTG+GGG 0.608345 1.2:+86206142 MS.gene74217:CDS
TATGGCAGCTCTCATGTAGT+CGG 0.609330 1.2:-86207426 None:intergenic
AAGTGCCAATCCAACACCAT+TGG 0.610021 1.2:-86205882 None:intergenic
TCCAAATCGATCAATGCCAA+TGG 0.610681 1.2:-86207999 None:intergenic
CAACTTTCAGACTTACACTC+CGG 0.611225 1.2:+86207136 MS.gene74217:intron
CCCATTGGAGCACAACCCAT+GGG 0.612164 1.2:-86205036 None:intergenic
GTCGAGTGTTTCGACAGCGG+TGG 0.613682 1.2:-86204914 None:intergenic
ATTGAAGGTGTTGAAAAGGG+TGG 0.614729 1.2:+86207704 MS.gene74217:CDS
GATTTCTTGTAGCATCAGCA+GGG 0.614806 1.2:-86207163 None:intergenic
AATAAGACCAGGAAGAACCT+TGG 0.616495 1.2:-86207213 None:intergenic
AATAACTCCGGCTTCACACA+AGG 0.617620 1.2:-86207459 None:intergenic
AAAGCTAATTCGGGTCACCC+GGG 0.618320 1.2:+86205009 MS.gene74217:CDS
CTACAAGGCAGAATCTTGGA+TGG 0.619439 1.2:+86206668 MS.gene74217:CDS
GGGTCTCTTCTGGTTAAGCA+CGG 0.620082 1.2:-86207630 None:intergenic
TCCAGCAGGGAAAATATACA+AGG 0.623690 1.2:+86208030 MS.gene74217:CDS
CATGTGATTTGGGTAAAGAA+CGG 0.627359 1.2:+86206274 MS.gene74217:CDS
TGAGAAGAAATACAAAGAGG+AGG 0.628944 1.2:+86206880 MS.gene74217:CDS
TGCTGGTTATGACAGTGTCA+AGG 0.631432 1.2:+86205179 MS.gene74217:CDS
TGCAGCTCCGTGAACACTGT+AGG 0.641746 1.2:-86206623 None:intergenic
TATGGAATTGAAGTCACCAC+AGG 0.643063 1.2:+86205724 MS.gene74217:CDS
TGAGGTATACGGCACGTGCG+AGG 0.647297 1.2:-86204756 None:intergenic
GTGCTGCACTCGAAGCTGAG+TGG 0.649503 1.2:+86206837 MS.gene74217:CDS
TTGGAGTCGCCATACCCCTG+AGG 0.652993 1.2:+86206814 MS.gene74217:CDS
CACAACCCATGGGTAAACCC+GGG 0.653503 1.2:-86205026 None:intergenic
TTTGTCGAGTGTTTCGACAG+CGG 0.655709 1.2:-86204917 None:intergenic
GCAATGGGAGCAATCTGCAA+TGG 0.655788 1.2:+86207347 MS.gene74217:CDS
TTGGAGCAAGTGCTCCAGCA+GGG 0.659920 1.2:+86208017 MS.gene74217:CDS
TTCGAGTGCAGCACCCTCAG+GGG 0.661757 1.2:-86206828 None:intergenic
GCACTGCCACCAATAAGACC+AGG 0.667283 1.2:-86207224 None:intergenic
CTTCAATAGAAGTTCCCGGA+AGG 0.667722 1.2:-86207688 None:intergenic
TTGAAGCAGTTTCGACAATG+GGG 0.670618 1.2:+86205664 MS.gene74217:CDS
ATTTCTTGTAGCATCAGCAG+GGG 0.674247 1.2:-86207162 None:intergenic
GTATGAGAAGAAATACAAAG+AGG 0.678342 1.2:+86206877 MS.gene74217:CDS
GACAGCTAGAGTCAGCATTG+AGG 0.680303 1.2:+86207928 MS.gene74217:CDS
CAAGGAGTATGGCATAACCA+AGG 0.693332 1.2:+86208048 MS.gene74217:CDS
TTTCCTTAGATCATCAGCGG+CGG 0.694677 1.2:-86207810 None:intergenic
TTGGCAATACCCTGACCCAG+AGG 0.713966 1.2:-86205863 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGATAAAATATTGAAATT+TGG - chr1.2:86205281-86205300 None:intergenic 10.0%
!! TAATCAATTTATCTATAACA+TGG - chr1.2:86206421-86206440 None:intergenic 15.0%
!! TATAGATAAATTGATTATAC+TGG + chr1.2:86206424-86206443 MS.gene74217:intron 15.0%
!!! GTTGTTATATCATTTTTATT+TGG + chr1.2:86206503-86206522 MS.gene74217:intron 15.0%
!! AATTTATCTATAACATGGAT+TGG - chr1.2:86206416-86206435 None:intergenic 20.0%
!! ATTTATCTATAACATGGATT+GGG - chr1.2:86206415-86206434 None:intergenic 20.0%
!! TTAAAATTGAAAATCTGCAA+TGG + chr1.2:86205372-86205391 MS.gene74217:intron 20.0%
!!! AAGATGTTAAAAAGTATGAA+TGG + chr1.2:86206716-86206735 MS.gene74217:intron 20.0%
!!! AATTTTGATTACTATTTGAC+AGG + chr1.2:86206558-86206577 MS.gene74217:intron 20.0%
!!! AGATGTTAAAAAGTATGAAT+GGG + chr1.2:86206717-86206736 MS.gene74217:intron 20.0%
!!! TTATCTTTTGCTTTTGTTTT+TGG + chr1.2:86206057-86206076 MS.gene74217:intron 20.0%
!!! TTTGGGTTGAATAAAATAAT+AGG + chr1.2:86205633-86205652 MS.gene74217:intron 20.0%
! AACAAGTAACAAGATGTAAT+TGG - chr1.2:86205979-86205998 None:intergenic 25.0%
! GATAATCACATATCTATTGA+TGG + chr1.2:86206193-86206212 MS.gene74217:CDS 25.0%
! TAGTAATGAAATTGTTAGTC+AGG - chr1.2:86205554-86205573 None:intergenic 25.0%
!! AAGTATTTTTCTCCTTTGTT+TGG - chr1.2:86205421-86205440 None:intergenic 25.0%
!!! TCATACTTTTTAACATCTTC+TGG - chr1.2:86206715-86206734 None:intergenic 25.0%
!!! TGTTATTTTTATAAGACCTG+TGG - chr1.2:86205743-86205762 None:intergenic 25.0%
!!! TTCTATTGCTTTTAAACTTG+TGG + chr1.2:86205317-86205336 MS.gene74217:intron 25.0%
!!! TTTCTATTGATTTTGTCTTG+AGG + chr1.2:86206990-86207009 MS.gene74217:intron 25.0%
AAAGTAAACCTTTCCTTTCT+TGG + chr1.2:86206478-86206497 MS.gene74217:intron 30.0%
AAGTAAACCTTTCCTTTCTT+GGG + chr1.2:86206479-86206498 MS.gene74217:intron 30.0%
ACCTAAACAACGAAAATACA+AGG - chr1.2:86205843-86205862 None:intergenic 30.0%
AGACAAACCTACTTTGATTA+AGG + chr1.2:86206351-86206370 MS.gene74217:CDS 30.0%
AGCAGTCAAAATTCAAAAGT+AGG - chr1.2:86207039-86207058 None:intergenic 30.0%
AGGTATGTTATATTAGGAGA+TGG + chr1.2:86206088-86206107 MS.gene74217:intron 30.0%
AGTTGAGAAGAGAAGTAAAT+TGG - chr1.2:86205255-86205274 None:intergenic 30.0%
ATAGGTTCAAATCCAAACAA+AGG + chr1.2:86205406-86205425 MS.gene74217:intron 30.0%
ATGACTTTGTTGAAATCGTT+TGG + chr1.2:86207266-86207285 MS.gene74217:CDS 30.0%
CAAGATCATGAAAATATCAG+TGG - chr1.2:86206765-86206784 None:intergenic 30.0%
CATTACAGAAGAAGCAAAAA+AGG - chr1.2:86205808-86205827 None:intergenic 30.0%
GGTCATAACATAAATAACTC+CGG - chr1.2:86207474-86207493 None:intergenic 30.0%
GTATGAGAAGAAATACAAAG+AGG + chr1.2:86206877-86206896 MS.gene74217:CDS 30.0%
GTTCCAATCAAAATAACATC+AGG - chr1.2:86207764-86207783 None:intergenic 30.0%
TAAACCAAGAAGGATTCTTT+GGG - chr1.2:86205095-86205114 None:intergenic 30.0%
TAAATGTCACATTACGAATC+AGG - chr1.2:86206040-86206059 None:intergenic 30.0%
TAATGAATTGATATGCACAG+TGG + chr1.2:86205574-86205593 MS.gene74217:intron 30.0%
TCACTAACCTTAATCAAAGT+AGG - chr1.2:86206361-86206380 None:intergenic 30.0%
TCTGAAAGTTGAAGTCAAAT+TGG - chr1.2:86207127-86207146 None:intergenic 30.0%
TGAAATTTATCTAATCCCAC+AGG - chr1.2:86205343-86205362 None:intergenic 30.0%
TGTGATCAATATTCCACAAA+TGG - chr1.2:86207101-86207120 None:intergenic 30.0%
TTAAACCAAGAAGGATTCTT+TGG - chr1.2:86205096-86205115 None:intergenic 30.0%
TTAAGGTTAGTGACATGAAA+TGG + chr1.2:86206368-86206387 MS.gene74217:intron 30.0%
! GTTGTTTCAGATAGCTTATA+TGG + chr1.2:86205467-86205486 MS.gene74217:intron 30.0%
! TCCTTGTATTTTCGTTGTTT+AGG + chr1.2:86205839-86205858 MS.gene74217:intron 30.0%
! TGCTCTAGGTATGTTATATT+AGG + chr1.2:86206082-86206101 MS.gene74217:intron 30.0%
!! GAGTAAAGTGTCATACTTTT+TGG + chr1.2:86206967-86206986 MS.gene74217:intron 30.0%
!! TCTATTGAAGGTGTTGAAAA+GGG + chr1.2:86207701-86207720 MS.gene74217:CDS 30.0%
!! TTCTATTGAAGGTGTTGAAA+AGG + chr1.2:86207700-86207719 MS.gene74217:CDS 30.0%
!! TTTTTATTTGGTTTCGATGC+TGG + chr1.2:86206515-86206534 MS.gene74217:intron 30.0%
!!! AAGCCTGATGTTATTTTGAT+TGG + chr1.2:86207758-86207777 MS.gene74217:CDS 30.0%
!!! AGTTGTTTCTTTTGTTAGCT+GGG + chr1.2:86207850-86207869 MS.gene74217:CDS 30.0%
!!! GATGTTATTTTGATTGGAAC+TGG + chr1.2:86207764-86207783 MS.gene74217:CDS 30.0%
AAAAAAGGCTCACAGTAACA+TGG - chr1.2:86205793-86205812 None:intergenic 35.0%
AAGTATGACACTTTACTCAC+CGG - chr1.2:86206965-86206984 None:intergenic 35.0%
AATTTCAGGGTTGAGGTATA+CGG - chr1.2:86204770-86204789 None:intergenic 35.0%
ACAAAGAAAGTTGCACAGTA+TGG - chr1.2:86207401-86207420 None:intergenic 35.0%
AGAGAAGTAAATTGGTTGGT+TGG - chr1.2:86205247-86205266 None:intergenic 35.0%
ATAACCCAAAGAATCCTTCT+TGG + chr1.2:86205088-86205107 MS.gene74217:CDS 35.0%
ATCGTTTGGAGATTTCCAAA+AGG + chr1.2:86207280-86207299 MS.gene74217:CDS 35.0%
ATTAGGAGATGGTTGTCAAA+TGG + chr1.2:86206099-86206118 MS.gene74217:CDS 35.0%
ATTGGTTATCTCACGTGTAA+TGG - chr1.2:86205961-86205980 None:intergenic 35.0%
CATAAGATTGTTGGAAGCAA+GGG + chr1.2:86207971-86207990 MS.gene74217:CDS 35.0%
CGATTTCAACAAAGTCATGT+TGG - chr1.2:86207264-86207283 None:intergenic 35.0%
CTAAAGAAGTAGATGCTACA+AGG + chr1.2:86206653-86206672 MS.gene74217:CDS 35.0%
GAAATGGAAATTCAAGCAAG+TGG + chr1.2:86206384-86206403 MS.gene74217:intron 35.0%
GAGAAGAGAAGTAAATTGGT+TGG - chr1.2:86205251-86205270 None:intergenic 35.0%
GATATAACAACACCCAAGAA+AGG - chr1.2:86206494-86206513 None:intergenic 35.0%
GGGAAAATATACAAGGAGTA+TGG + chr1.2:86208037-86208056 MS.gene74217:CDS 35.0%
GTTATGACCCATGATTCAAT+TGG + chr1.2:86207485-86207504 MS.gene74217:CDS 35.0%
TAACCTGCTTAACATTCCTT+AGG + chr1.2:86206789-86206808 MS.gene74217:intron 35.0%
TCAAAACATTTGGCATTGCT+CGG - chr1.2:86207562-86207581 None:intergenic 35.0%
TCCAAGTCCAATTGAATCAT+GGG - chr1.2:86207495-86207514 None:intergenic 35.0%
TGACATAATGTGACTGTTGA+GGG + chr1.2:86206453-86206472 MS.gene74217:intron 35.0%
TGAGAAGAAATACAAAGAGG+AGG + chr1.2:86206880-86206899 MS.gene74217:CDS 35.0%
TGAGTTCTATCAACCATTTG+TGG + chr1.2:86207085-86207104 MS.gene74217:intron 35.0%
TGATCAGTCAGATGAATACA+AGG + chr1.2:86207883-86207902 MS.gene74217:CDS 35.0%
TGCTGTTGAGAAAGCTAATT+CGG + chr1.2:86204999-86205018 MS.gene74217:CDS 35.0%
TTCTTTCCTTAGATCATCAG+CGG - chr1.2:86207816-86207835 None:intergenic 35.0%
TTGAAAATCTGCAATGGAGT+TGG + chr1.2:86205378-86205397 MS.gene74217:intron 35.0%
TTTGGTTGGCATAAGATTGT+TGG + chr1.2:86207962-86207981 MS.gene74217:CDS 35.0%
! CATGTGATTTGGGTAAAGAA+CGG + chr1.2:86206274-86206293 MS.gene74217:CDS 35.0%
! GAATGTTGATCAACGTTTTG+AGG + chr1.2:86206240-86206259 MS.gene74217:CDS 35.0%
! TTTTAACATCTTCTGGCACA+TGG - chr1.2:86206708-86206727 None:intergenic 35.0%
!! CTGTAGGAATTAGCTTTGTT+TGG - chr1.2:86206610-86206629 None:intergenic 35.0%
!! GAGCTGAATTTGTTGTGTTT+GGG + chr1.2:86205616-86205635 MS.gene74217:intron 35.0%
!! TGAGCTGAATTTGTTGTGTT+TGG + chr1.2:86205615-86205634 MS.gene74217:intron 35.0%
!!! GAGTTGTTTCTTTTGTTAGC+TGG + chr1.2:86207849-86207868 MS.gene74217:CDS 35.0%
!!! TTTTGTTTTTGGTCTGCTCT+AGG + chr1.2:86206068-86206087 MS.gene74217:intron 35.0%
AAAATCTGCAATGGAGTTGG+AGG + chr1.2:86205381-86205400 MS.gene74217:intron 40.0%
AACAACACCCAAGAAAGGAA+AGG - chr1.2:86206489-86206508 None:intergenic 40.0%
AATAAGACCAGGAAGAACCT+TGG - chr1.2:86207216-86207235 None:intergenic 40.0%
ACACAAGGCAGAAATCCTTA+TGG - chr1.2:86207447-86207466 None:intergenic 40.0%
ACACCTTCAATAGAAGTTCC+CGG - chr1.2:86207695-86207714 None:intergenic 40.0%
ACAGCAAAATCTAAATGCCC+TGG + chr1.2:86207190-86207209 MS.gene74217:CDS 40.0%
ACCATTTCAGACAACTCATC+AGG + chr1.2:86207731-86207750 MS.gene74217:CDS 40.0%
ACCCATGATTCAATTGGACT+TGG + chr1.2:86207491-86207510 MS.gene74217:CDS 40.0%
ACCTGATGAGTTGTCTGAAA+TGG - chr1.2:86207735-86207754 None:intergenic 40.0%
ACTTGAAGCAGTTTCGACAA+TGG + chr1.2:86205662-86205681 MS.gene74217:CDS 40.0%
ACTTGGATGGCATGTGATTT+GGG + chr1.2:86206264-86206283 MS.gene74217:CDS 40.0%
AGAACCAAATCCAATGGTAG+TGG - chr1.2:86206587-86206606 None:intergenic 40.0%
AGATTTCTTGTAGCATCAGC+AGG - chr1.2:86207167-86207186 None:intergenic 40.0%
AGCAGAAATAACAGCTTCCT+TGG - chr1.2:86208068-86208087 None:intergenic 40.0%
ATCGTGAATTACCTGCTGAT+TGG + chr1.2:86206927-86206946 MS.gene74217:CDS 40.0%
ATGCCTAAGGAATGTTAAGC+AGG - chr1.2:86206795-86206814 None:intergenic 40.0%
ATTTCTTGTAGCATCAGCAG+GGG - chr1.2:86207165-86207184 None:intergenic 40.0%
CAACAAAGTCATGTTGGAAG+AGG - chr1.2:86207258-86207277 None:intergenic 40.0%
CAACTTTCAGACTTACACTC+CGG + chr1.2:86207136-86207155 MS.gene74217:intron 40.0%
CGATCACGATTAAACCAAGA+AGG - chr1.2:86205105-86205124 None:intergenic 40.0%
CTCCAAGTCCAATTGAATCA+TGG - chr1.2:86207496-86207515 None:intergenic 40.0%
CTTGAAGCAGTTTCGACAAT+GGG + chr1.2:86205663-86205682 MS.gene74217:CDS 40.0%
GATTTCTTGTAGCATCAGCA+GGG - chr1.2:86207166-86207185 None:intergenic 40.0%
GCATAAGATTGTTGGAAGCA+AGG + chr1.2:86207970-86207989 MS.gene74217:CDS 40.0%
GCTAATTCCTACAGTGTTCA+CGG + chr1.2:86206616-86206635 MS.gene74217:CDS 40.0%
GCTGATGATCTAAGGAAAGA+AGG + chr1.2:86207815-86207834 MS.gene74217:CDS 40.0%
GCTGTTGAGAAAGCTAATTC+GGG + chr1.2:86205000-86205019 MS.gene74217:CDS 40.0%
GCTTAACATTCCTTAGGCAT+TGG + chr1.2:86206795-86206814 MS.gene74217:intron 40.0%
GTGACATAATGTGACTGTTG+AGG + chr1.2:86206452-86206471 MS.gene74217:intron 40.0%
GTTACCACTACCATTGGATT+TGG + chr1.2:86206580-86206599 MS.gene74217:CDS 40.0%
GTTTGGAGAACCAAATCCAA+TGG - chr1.2:86206593-86206612 None:intergenic 40.0%
TATGCTCTTCTTCATCTTGC+TGG + chr1.2:86205162-86205181 MS.gene74217:CDS 40.0%
TATGGAATTGAAGTCACCAC+AGG + chr1.2:86205724-86205743 MS.gene74217:CDS 40.0%
TCCAAATCGATCAATGCCAA+TGG - chr1.2:86208002-86208021 None:intergenic 40.0%
TCCAGCAGGGAAAATATACA+AGG + chr1.2:86208030-86208049 MS.gene74217:CDS 40.0%
TCCATATGTCTCGAAGTTCT+CGG - chr1.2:86205710-86205729 None:intergenic 40.0%
TCGTATAGAATGTGACCCAT+TGG - chr1.2:86205054-86205073 None:intergenic 40.0%
TGAAATTCGTGCAGCCATTA+AGG + chr1.2:86206312-86206331 MS.gene74217:CDS 40.0%
TGATTCAATTGGACTTGGAG+AGG + chr1.2:86207496-86207515 MS.gene74217:CDS 40.0%
TGGGTAAAGAACGGAAATAC+TGG + chr1.2:86206283-86206302 MS.gene74217:CDS 40.0%
TTGAAGCAGTTTCGACAATG+GGG + chr1.2:86205664-86205683 MS.gene74217:CDS 40.0%
TTTCGTTGTTTAGGTCCTCT+GGG + chr1.2:86205848-86205867 MS.gene74217:intron 40.0%
TTTCTCAACGAGAGAAGCTT+CGG - chr1.2:86204947-86204966 None:intergenic 40.0%
! AGTGATGATGCACGAATTTC+AGG - chr1.2:86204784-86204803 None:intergenic 40.0%
! CTTTTAAACTTGTGGCCTGT+GGG + chr1.2:86205325-86205344 MS.gene74217:intron 40.0%
! GGACGAAGCATCAAAACATT+TGG - chr1.2:86207572-86207591 None:intergenic 40.0%
! GTGATGATGCACGAATTTCA+GGG - chr1.2:86204783-86204802 None:intergenic 40.0%
! TATTGCCAATGGTGTTGGAT+TGG + chr1.2:86205877-86205896 MS.gene74217:CDS 40.0%
! TCCTTGTATATTTTCCCTGC+TGG - chr1.2:86208034-86208053 None:intergenic 40.0%
! TCGTGAATTACCTGCTGATT+GGG + chr1.2:86206928-86206947 MS.gene74217:CDS 40.0%
! TTTTCGTTGTTTAGGTCCTC+TGG + chr1.2:86205847-86205866 MS.gene74217:intron 40.0%
!! AAGGTTCTTCCTGGTCTTAT+TGG + chr1.2:86207215-86207234 MS.gene74217:CDS 40.0%
!! ACCATTGGCATTGATCGATT+TGG + chr1.2:86207998-86208017 MS.gene74217:CDS 40.0%
!! ATTGAAGGTGTTGAAAAGGG+TGG + chr1.2:86207704-86207723 MS.gene74217:CDS 40.0%
!! CAATGTTCGATTTGGTGTCA+GGG + chr1.2:86207319-86207338 MS.gene74217:CDS 40.0%
!!! TTTGTTTTGTCTGCTGGACA+TGG + chr1.2:86205126-86205145 MS.gene74217:CDS 40.0%
AAATCTAAATGCCCTGGCCA+AGG + chr1.2:86207196-86207215 MS.gene74217:CDS 45.0%
AAGAGGAGGATTTGAGTGCA+GGG - chr1.2:86204831-86204850 None:intergenic 45.0%
AAGTGCCAATCCAACACCAT+TGG - chr1.2:86205885-86205904 None:intergenic 45.0%
AATAACTCCGGCTTCACACA+AGG - chr1.2:86207462-86207481 None:intergenic 45.0%
AATCGAACATTGCGCTCTTC+TGG - chr1.2:86207311-86207330 None:intergenic 45.0%
AATGAAGCGTGTTCACTTGC+CGG + chr1.2:86206133-86206152 MS.gene74217:CDS 45.0%
ACATCTTCTGGCACATGGAA+AGG - chr1.2:86206703-86206722 None:intergenic 45.0%
ACTACATGAGAGCTGCCATA+AGG + chr1.2:86207429-86207448 MS.gene74217:CDS 45.0%
ATAGAAGTTCCCGGAAGGTT+AGG - chr1.2:86207686-86207705 None:intergenic 45.0%
ATGCACGAATTTCAGGGTTG+AGG - chr1.2:86204777-86204796 None:intergenic 45.0%
ATTGAGGCGGGATCAACATT+TGG + chr1.2:86207944-86207963 MS.gene74217:CDS 45.0%
ATTTCTGCCTTGTGTGAAGC+CGG + chr1.2:86207452-86207471 MS.gene74217:CDS 45.0%
CAAAAGTTGCCTAACCTTCC+GGG + chr1.2:86207674-86207693 MS.gene74217:CDS 45.0%
CAAGAATTGAGGGTCTCTTC+TGG - chr1.2:86207643-86207662 None:intergenic 45.0%
CACAGTATGGAATGAATCCC+GGG - chr1.2:86207388-86207407 None:intergenic 45.0%
CACTTGGATGGCATGTGATT+TGG + chr1.2:86206263-86206282 MS.gene74217:CDS 45.0%
CCATATGTCTCGAAGTTCTC+GGG - chr1.2:86205709-86205728 None:intergenic 45.0%
CCTCAATTCTTGCCCTTTCT+CGG + chr1.2:86207651-86207670 MS.gene74217:CDS 45.0%
CGAGAAAGGGCAAGAATTGA+GGG - chr1.2:86207653-86207672 None:intergenic 45.0%
CTACAAGGCAGAATCTTGGA+TGG + chr1.2:86206668-86206687 MS.gene74217:CDS 45.0%
CTTCAATAGAAGTTCCCGGA+AGG - chr1.2:86207691-86207710 None:intergenic 45.0%
CTTCCGGGAACTTCTATTGA+AGG + chr1.2:86207689-86207708 MS.gene74217:CDS 45.0%
GACAGCCTTTGCTTCCTTAA+TGG - chr1.2:86206329-86206348 None:intergenic 45.0%
GATGCTACAAGGCAGAATCT+TGG + chr1.2:86206664-86206683 MS.gene74217:CDS 45.0%
GCAAAAGTTGCCTAACCTTC+CGG + chr1.2:86207673-86207692 MS.gene74217:CDS 45.0%
GCACAGTATGGAATGAATCC+CGG - chr1.2:86207389-86207408 None:intergenic 45.0%
GCAGTTTCATTACCATCAGC+TGG - chr1.2:86207593-86207612 None:intergenic 45.0%
GGAAGCAAGGGAAAAACCAT+TGG + chr1.2:86207983-86208002 MS.gene74217:CDS 45.0%
GGAGATGGTTGTCAAATGGA+AGG + chr1.2:86206103-86206122 MS.gene74217:CDS 45.0%
TATGGCAGCTCTCATGTAGT+CGG - chr1.2:86207429-86207448 None:intergenic 45.0%
TCAATTGGACTTGGAGAGGA+TGG + chr1.2:86207500-86207519 MS.gene74217:CDS 45.0%
TGACAGGTTACCACTACCAT+TGG + chr1.2:86206574-86206593 MS.gene74217:intron 45.0%
TGCAGCCATTAAGGAAGCAA+AGG + chr1.2:86206321-86206340 MS.gene74217:CDS 45.0%
TGGTATTGCTCTTCATAGCC+CGG + chr1.2:86207367-86207386 MS.gene74217:CDS 45.0%
TGGTCAACAATCTCACTGTC+AGG - chr1.2:86205941-86205960 None:intergenic 45.0%
TTTCCTTAGATCATCAGCGG+CGG - chr1.2:86207813-86207832 None:intergenic 45.0%
TTTGTCGAGTGTTTCGACAG+CGG - chr1.2:86204920-86204939 None:intergenic 45.0%
! AGGCAACTTTTGCCGAGAAA+GGG - chr1.2:86207666-86207685 None:intergenic 45.0%
! CAAGGAGTATGGCATAACCA+AGG + chr1.2:86208048-86208067 MS.gene74217:CDS 45.0%
! GAAGAGCGCAATGTTCGATT+TGG + chr1.2:86207311-86207330 MS.gene74217:CDS 45.0%
! GCTGATGGTAATGAAACTGC+TGG + chr1.2:86207593-86207612 MS.gene74217:CDS 45.0%
! GCTTTTAAACTTGTGGCCTG+TGG + chr1.2:86205324-86205343 MS.gene74217:intron 45.0%
! TAGGCAACTTTTGCCGAGAA+AGG - chr1.2:86207667-86207686 None:intergenic 45.0%
! TGCTCTATTGGCTGATGAGT+TGG - chr1.2:86207527-86207546 None:intergenic 45.0%
! TGCTGGTTATGACAGTGTCA+AGG + chr1.2:86205179-86205198 MS.gene74217:CDS 45.0%
!! GATTGGAACTGGTTCTGAGT+TGG + chr1.2:86207775-86207794 MS.gene74217:CDS 45.0%
!! GCAATGTTCGATTTGGTGTC+AGG + chr1.2:86207318-86207337 MS.gene74217:CDS 45.0%
!! GTTCTTCCTGGTCTTATTGG+TGG + chr1.2:86207218-86207237 MS.gene74217:CDS 45.0%
!! TCTCAACAGCATCGATAGCA+AGG - chr1.2:86204990-86205009 None:intergenic 45.0%
!! TGATTGGGAGAAAGCACTTC+CGG + chr1.2:86206943-86206962 MS.gene74217:CDS 45.0%
!!! AACGTTTTGAGGCACTTGGA+TGG + chr1.2:86206251-86206270 MS.gene74217:CDS 45.0%
!!! GATCAACGTTTTGAGGCACT+TGG + chr1.2:86206247-86206266 MS.gene74217:CDS 45.0%
!!! GATCGCTTTGTTTTGTCTGC+TGG + chr1.2:86205120-86205139 MS.gene74217:CDS 45.0%
AAAGCTAATTCGGGTCACCC+GGG + chr1.2:86205009-86205028 MS.gene74217:CDS 50.0%
AAGCTTGCCAGGTGCTCTAT+TGG - chr1.2:86207539-86207558 None:intergenic 50.0%
ACTCTAGCTGTCACAGAAGC+AGG - chr1.2:86207920-86207939 None:intergenic 50.0%
AGGCGGGATCAACATTTGGT+TGG + chr1.2:86207948-86207967 MS.gene74217:CDS 50.0%
AGTGCTTTCTCCCAATCAGC+AGG - chr1.2:86206941-86206960 None:intergenic 50.0%
AGTGTTCACGGAGCTGCATT+GGG + chr1.2:86206628-86206647 MS.gene74217:CDS 50.0%
ATTTGAGTGCAGGGAAGGAG+AGG - chr1.2:86204822-86204841 None:intergenic 50.0%
CAATGGGGAAGCAAAACTCC+TGG + chr1.2:86205679-86205698 MS.gene74217:CDS 50.0%
CCCGAGAACTTCGAGACATA+TGG + chr1.2:86205706-86205725 MS.gene74217:CDS 50.0%
CCGAGAAAGGGCAAGAATTG+AGG - chr1.2:86207654-86207673 None:intergenic 50.0%
CTCTAGCTGTCACAGAAGCA+GGG - chr1.2:86207919-86207938 None:intergenic 50.0%
CTTTCCAAGACCCCAATGTC+CGG - chr1.2:86206155-86206174 None:intergenic 50.0%
GAAAGCTAATTCGGGTCACC+CGG + chr1.2:86205008-86205027 MS.gene74217:CDS 50.0%
GAAGAGGAGGATTTGAGTGC+AGG - chr1.2:86204832-86204851 None:intergenic 50.0%
GACAGCTAGAGTCAGCATTG+AGG + chr1.2:86207928-86207947 MS.gene74217:CDS 50.0%
GCAATGGAGTTGGAGGTCAT+AGG + chr1.2:86205388-86205407 MS.gene74217:intron 50.0%
GCAATGGGAGCAATCTGCAA+TGG + chr1.2:86207347-86207366 MS.gene74217:CDS 50.0%
GGCACATGGAAAGGCTCATA+TGG - chr1.2:86206694-86206713 None:intergenic 50.0%
GGTATTGCTCTTCATAGCCC+GGG + chr1.2:86207368-86207387 MS.gene74217:CDS 50.0%
GTGTTCACTTGCCGGACATT+GGG + chr1.2:86206141-86206160 MS.gene74217:CDS 50.0%
TATGGCGACTCCAATGCCTA+AGG - chr1.2:86206808-86206827 None:intergenic 50.0%
TGGTTTCGATGCTGGTCACA+TGG + chr1.2:86206523-86206542 MS.gene74217:intron 50.0%
TGTTCACTTGCCGGACATTG+GGG + chr1.2:86206142-86206161 MS.gene74217:CDS 50.0%
TGTTTAGGTCCTCTGGGTCA+GGG + chr1.2:86205854-86205873 MS.gene74217:intron 50.0%
TTGATGCTTCGTCCAGCTGA+TGG + chr1.2:86207578-86207597 MS.gene74217:CDS 50.0%
TTGTTTAGGTCCTCTGGGTC+AGG + chr1.2:86205853-86205872 MS.gene74217:intron 50.0%
TTTGAGTGCAGGGAAGGAGA+GGG - chr1.2:86204821-86204840 None:intergenic 50.0%
! AGCTAGAGTCAGCATTGAGG+CGG + chr1.2:86207931-86207950 MS.gene74217:CDS 50.0%
! GGGTCTCTTCTGGTTAAGCA+CGG - chr1.2:86207633-86207652 None:intergenic 50.0%
! TCATCAGCCAATAGAGCACC+TGG + chr1.2:86207529-86207548 MS.gene74217:CDS 50.0%
!! CAGGGTATTGCCAATGGTGT+TGG + chr1.2:86205872-86205891 MS.gene74217:CDS 50.0%
!! TGCTGCTGGTTTGCATGAAG+AGG - chr1.2:86204848-86204867 None:intergenic 50.0%
!! TGCTGGTTTGCATGAAGAGG+AGG - chr1.2:86204845-86204864 None:intergenic 50.0%
!!! GCTCTTCTGGAGTAGCCTTT+TGG - chr1.2:86207298-86207317 None:intergenic 50.0%
ACCAGGAAGAACCTTGGCCA+GGG - chr1.2:86207210-86207229 None:intergenic 55.0%
ACCCATTGGAGCACAACCCA+TGG - chr1.2:86205040-86205059 None:intergenic 55.0%
AGAGGAGGCTGCAGAGCTAA+AGG + chr1.2:86206895-86206914 MS.gene74217:CDS 55.0%
AGGGAAGGAGAGGGAAGAAG+TGG - chr1.2:86204812-86204831 None:intergenic 55.0%
CACAACCCATGGGTAAACCC+GGG - chr1.2:86205029-86205048 None:intergenic 55.0%
CAGTGTTCACGGAGCTGCAT+TGG + chr1.2:86206627-86206646 MS.gene74217:CDS 55.0%
CCCATTGGAGCACAACCCAT+GGG - chr1.2:86205039-86205058 None:intergenic 55.0%
CGTGTTCACTTGCCGGACAT+TGG + chr1.2:86206140-86206159 MS.gene74217:CDS 55.0%
CTCAACGAGAGAAGCTTCGG+TGG - chr1.2:86204944-86204963 None:intergenic 55.0%
GAAGGAGAGGGAAGAAGTGG+AGG - chr1.2:86204809-86204828 None:intergenic 55.0%
GCACAACCCATGGGTAAACC+CGG - chr1.2:86205030-86205049 None:intergenic 55.0%
GCACTGCCACCAATAAGACC+AGG - chr1.2:86207227-86207246 None:intergenic 55.0%
GGAGGATTTGAGTGCAGGGA+AGG - chr1.2:86204827-86204846 None:intergenic 55.0%
GGTGTCAGGGAACACGCAAT+GGG + chr1.2:86207332-86207351 MS.gene74217:CDS 55.0%
GTAGCATCAGCAGGGGTTTC+CGG - chr1.2:86207158-86207177 None:intergenic 55.0%
TCGAAGTTCTCGGGATGTCC+AGG - chr1.2:86205700-86205719 None:intergenic 55.0%
TGCAGCTCCGTGAACACTGT+AGG - chr1.2:86206626-86206645 None:intergenic 55.0%
TTGGAGCAAGTGCTCCAGCA+GGG + chr1.2:86208017-86208036 MS.gene74217:CDS 55.0%
TTGGCAATACCCTGACCCAG+AGG - chr1.2:86205866-86205885 None:intergenic 55.0%
! ACCCATGGGTTGTGCTCCAA+TGG + chr1.2:86205035-86205054 MS.gene74217:CDS 55.0%
! CCCATGGGTTGTGCTCCAAT+GGG + chr1.2:86205036-86205055 MS.gene74217:CDS 55.0%
! GCTAGAGTCAGCATTGAGGC+GGG + chr1.2:86207932-86207951 MS.gene74217:CDS 55.0%
! TGGTGTCAGGGAACACGCAA+TGG + chr1.2:86207331-86207350 MS.gene74217:CDS 55.0%
! TTTGGAGCAAGTGCTCCAGC+AGG + chr1.2:86208016-86208035 MS.gene74217:CDS 55.0%
!! CTGGGTCAGGGTATTGCCAA+TGG + chr1.2:86205866-86205885 MS.gene74217:CDS 55.0%
CCGTGCAGCCAAGTGCTTCT+CGG - chr1.2:86205909-86205928 None:intergenic 60.0%
CTGCCGCCGCTGATGATCTA+AGG + chr1.2:86207807-86207826 MS.gene74217:CDS 60.0%
CTTCGAGTGCAGCACCCTCA+GGG - chr1.2:86206832-86206851 None:intergenic 60.0%
CTTGCCGGACATTGGGGTCT+TGG + chr1.2:86206148-86206167 MS.gene74217:CDS 60.0%
GACCAGGAAGAACCTTGGCC+AGG - chr1.2:86207211-86207230 None:intergenic 60.0%
GCATTGCTCGGAAGCTTGCC+AGG - chr1.2:86207550-86207569 None:intergenic 60.0%
GGTCACCCGGGTTTACCCAT+GGG + chr1.2:86205021-86205040 MS.gene74217:CDS 60.0%
GTCGAGTGTTTCGACAGCGG+TGG - chr1.2:86204917-86204936 None:intergenic 60.0%
GTGCTGCACTCGAAGCTGAG+TGG + chr1.2:86206837-86206856 MS.gene74217:CDS 60.0%
TAGATCATCAGCGGCGGCAG+CGG - chr1.2:86207807-86207826 None:intergenic 60.0%
TGAGGTATACGGCACGTGCG+AGG - chr1.2:86204759-86204778 None:intergenic 60.0%
TGGAGTCGCCATACCCCTGA+GGG + chr1.2:86206815-86206834 MS.gene74217:CDS 60.0%
TTCGAGTGCAGCACCCTCAG+GGG - chr1.2:86206831-86206850 None:intergenic 60.0%
TTGGAGTCGCCATACCCCTG+AGG + chr1.2:86206814-86206833 MS.gene74217:CDS 60.0%
! TGGCTGGTGTGGTTCTGCGA+CGG - chr1.2:86204888-86204907 None:intergenic 60.0%
! TGTGGTTCTGCGACGGGAAC+CGG - chr1.2:86204881-86204900 None:intergenic 60.0%
!! CCGAGAAGCACTTGGCTGCA+CGG + chr1.2:86205906-86205925 MS.gene74217:CDS 60.0%
!! GGCACTTGCCGAGAAGCACT+TGG + chr1.2:86205898-86205917 MS.gene74217:CDS 60.0%
CCAGCAGCAACCTCCTCAGC+CGG + chr1.2:86204859-86204878 MS.gene74217:CDS 65.0%
GCCCTGGCCAAGGTTCTTCC+TGG + chr1.2:86207206-86207225 MS.gene74217:CDS 65.0%
GCTTCGAGTGCAGCACCCTC+AGG - chr1.2:86206833-86206852 None:intergenic 65.0%
GGGTCACCCGGGTTTACCCA+TGG + chr1.2:86205020-86205039 MS.gene74217:CDS 65.0%
GTGCAGCACCCTCAGGGGTA+TGG - chr1.2:86206826-86206845 None:intergenic 65.0%
GTGTTTCGACAGCGGTGGCG+CGG - chr1.2:86204912-86204931 None:intergenic 65.0%
! GGCTGGTGTGGTTCTGCGAC+GGG - chr1.2:86204887-86204906 None:intergenic 65.0%
CCGGCTGAGGAGGTTGCTGC+TGG - chr1.2:86204862-86204881 None:intergenic 70.0%
TCTGCGACGGGAACCGGCTG+AGG - chr1.2:86204875-86204894 None:intergenic 70.0%
TTCGACAGCGGTGGCGCGGA+TGG - chr1.2:86204908-86204927 None:intergenic 70.0%
ACAGCGGTGGCGCGGATGGC+TGG - chr1.2:86204904-86204923 None:intergenic 75.0%
GCGACGGGAACCGGCTGAGG+AGG - chr1.2:86204872-86204891 None:intergenic 75.0%
GGTGGCGCGGATGGCTGGTG+TGG - chr1.2:86204899-86204918 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 86204718 86208105 86204718 ID=MS.gene74217
chr1.2 mRNA 86204718 86208105 86204718 ID=MS.gene74217.t1;Parent=MS.gene74217
chr1.2 exon 86204718 86205200 86204718 ID=MS.gene74217.t1.exon1;Parent=MS.gene74217.t1
chr1.2 CDS 86204718 86205200 86204718 ID=cds.MS.gene74217.t1;Parent=MS.gene74217.t1
chr1.2 exon 86205655 86205745 86205655 ID=MS.gene74217.t1.exon2;Parent=MS.gene74217.t1
chr1.2 CDS 86205655 86205745 86205655 ID=cds.MS.gene74217.t1;Parent=MS.gene74217.t1
chr1.2 exon 86205861 86205966 86205861 ID=MS.gene74217.t1.exon3;Parent=MS.gene74217.t1
chr1.2 CDS 86205861 86205966 86205861 ID=cds.MS.gene74217.t1;Parent=MS.gene74217.t1
chr1.2 exon 86206090 86206372 86206090 ID=MS.gene74217.t1.exon4;Parent=MS.gene74217.t1
chr1.2 CDS 86206090 86206372 86206090 ID=cds.MS.gene74217.t1;Parent=MS.gene74217.t1
chr1.2 exon 86206580 86206728 86206580 ID=MS.gene74217.t1.exon5;Parent=MS.gene74217.t1
chr1.2 CDS 86206580 86206728 86206580 ID=cds.MS.gene74217.t1;Parent=MS.gene74217.t1
chr1.2 exon 86206811 86206964 86206811 ID=MS.gene74217.t1.exon6;Parent=MS.gene74217.t1
chr1.2 CDS 86206811 86206964 86206811 ID=cds.MS.gene74217.t1;Parent=MS.gene74217.t1
chr1.2 exon 86207146 86208105 86207146 ID=MS.gene74217.t1.exon7;Parent=MS.gene74217.t1
chr1.2 CDS 86207146 86208105 86207146 ID=cds.MS.gene74217.t1;Parent=MS.gene74217.t1
Gene Sequence

>MS.gene74217

ATGGCTTCTTCTTCTTCTCTTCATCTTTCTCAAGCATTCCTCGCACGTGCCGTATACCTCAACCCTGAAATTCGTGCATCATCACTCTCCTCCACTTCTTCCCTCTCCTTCCCTGCACTCAAATCCTCCTCTTCATGCAAACCAGCAGCAACCTCCTCAGCCGGTTCCCGTCGCAGAACCACACCAGCCATCCGCGCCACCGCTGTCGAAACACTCGACAAAACCACCGAAGCTTCTCTCGTTGAGAAATCAGTCAACACTATTCGATTCCTTGCTATCGATGCTGTTGAGAAAGCTAATTCGGGTCACCCGGGTTTACCCATGGGTTGTGCTCCAATGGGTCACATTCTATACGATGAAGTTATGAGATATAACCCAAAGAATCCTTCTTGGTTTAATCGTGATCGCTTTGTTTTGTCTGCTGGACATGGTTGTATGCTTCAGTATGCTCTTCTTCATCTTGCTGGTTATGACAGTGTCAAGGTTCTCTTTCAACTCAATCACTGTTTTTTACTCTTTAAACTAACCAACCAACCAATTTACTTCTCTTCTCAACTTAACCAAATTTCAATATTTTATCTTAAAATTAAACTGAATTTTTCTATTGCTTTTAAACTTGTGGCCTGTGGGATTAGATAAATTTCAAATAGTATATTAAAATTGAAAATCTGCAATGGAGTTGGAGGTCATAGGTTCAAATCCAAACAAAGGAGAAAAATACTTACATAACAAACTAATTACTAACATTCGTTGTTTCAGATAGCTTATATGGCAGAAATTTTGTTAAGTTTAGATATCATTTTGAAATTTCGTTAATTTTAAAGATAGAATTTCCTGACTAACAATTTCATTACTATAATGAATTGATATGCACAGTGGAATCTTTCATTTATTGACTGAGCTGAATTTGTTGTGTTTGGGTTGAATAAAATAATAGGAAGAAGACTTGAAGCAGTTTCGACAATGGGGAAGCAAAACTCCTGGACATCCCGAGAACTTCGAGACATATGGAATTGAAGTCACCACAGGTCTTATAAAAATAACAATATTGATAATAATATGTCTCTTTAGTCCATGTTACTGTGAGCCTTTTTTGCTTCTTCTGTAATGAATTTCAACTATCCTTGTATTTTCGTTGTTTAGGTCCTCTGGGTCAGGGTATTGCCAATGGTGTTGGATTGGCACTTGCCGAGAAGCACTTGGCTGCACGGTTTAACAAGCCTGACAGTGAGATTGTTGACCATTACACGTGAGATAACCAATTACATCTTGTTACTTGTTGATCTATACATGCTTGCACTTGTGAATTGCACTGTTTTCCTGATTCGTAATGTGACATTTATCTTTTGCTTTTGTTTTTGGTCTGCTCTAGGTATGTTATATTAGGAGATGGTTGTCAAATGGAAGGAATTTCAAATGAAGCGTGTTCACTTGCCGGACATTGGGGTCTTGGAAAGCTTATTGCATTTTATGATGATAATCACATATCTATTGATGGTGACACTGAAATTGCATTCACTGAGAATGTTGATCAACGTTTTGAGGCACTTGGATGGCATGTGATTTGGGTAAAGAACGGAAATACTGGATATGATGAAATTCGTGCAGCCATTAAGGAAGCAAAGGCTGTCACAGACAAACCTACTTTGATTAAGGTTAGTGACATGAAATGGAAATTCAAGCAAGTGGATTTACCCAATCCATGTTATAGATAAATTGATTATACTGGTTGAAGTGACATAATGTGACTGTTGAGGGTGAAAGTAAACCTTTCCTTTCTTGGGTGTTGTTATATCATTTTTATTTGGTTTCGATGCTGGTCACATGGTAGTATTTCATTAATTTTGATTACTATTTGACAGGTTACCACTACCATTGGATTTGGTTCTCCAAACAAAGCTAATTCCTACAGTGTTCACGGAGCTGCATTGGGTTCTAAAGAAGTAGATGCTACAAGGCAGAATCTTGGATGGCCATATGAGCCTTTCCATGTGCCAGAAGATGTTAAAAAGTATGAATGGGAGCATTAGTTATATGTTTGTTTCCACTGATATTTTCATGATCTTGATTATAACCTGCTTAACATTCCTTAGGCATTGGAGTCGCCATACCCCTGAGGGTGCTGCACTCGAAGCTGAGTGGAATGCTAAATTTGCTGAGTATGAGAAGAAATACAAAGAGGAGGCTGCAGAGCTAAAGGCTATTATTAATCGTGAATTACCTGCTGATTGGGAGAAAGCACTTCCGGTGAGTAAAGTGTCATACTTTTTGGTTTCTATTGATTTTGTCTTGAGGTATCTCTTCTGTAGTTGTTTGATCCTACTTTTGAATTTTGACTGCTTTTGAAGTTTTAAACTAAACAAAACTTGAGTTCTATCAACCATTTGTGGAATATTGATCACAATTCCAATTTGACTTCAACTTTCAGACTTACACTCCGGAAACCCCTGCTGATGCTACAAGAAATCTGTCACAGCAAAATCTAAATGCCCTGGCCAAGGTTCTTCCTGGTCTTATTGGTGGCAGTGCAGATCTTGCCTCTTCCAACATGACTTTGTTGAAATCGTTTGGAGATTTCCAAAAGGCTACTCCAGAAGAGCGCAATGTTCGATTTGGTGTCAGGGAACACGCAATGGGAGCAATCTGCAATGGTATTGCTCTTCATAGCCCGGGATTCATTCCATACTGTGCAACTTTCTTTGTCTTCACCGACTACATGAGAGCTGCCATAAGGATTTCTGCCTTGTGTGAAGCCGGAGTTATTTATGTTATGACCCATGATTCAATTGGACTTGGAGAGGATGGACCAACTCATCAGCCAATAGAGCACCTGGCAAGCTTCCGAGCAATGCCAAATGTTTTGATGCTTCGTCCAGCTGATGGTAATGAAACTGCTGGATCATACAGAGTTGCCGTGCTTAACCAGAAGAGACCCTCAATTCTTGCCCTTTCTCGGCAAAAGTTGCCTAACCTTCCGGGAACTTCTATTGAAGGTGTTGAAAAGGGTGGCTACACCATTTCAGACAACTCATCAGGTAACAAGCCTGATGTTATTTTGATTGGAACTGGTTCTGAGTTGGAAATTGCCGCTGCCGCCGCTGATGATCTAAGGAAAGAAGGAAAAACTGTAAGAGTTGTTTCTTTTGTTAGCTGGGAACTTTTTGATGATCAGTCAGATGAATACAAGGAGAGTGTTCTCCCTGCTTCTGTGACAGCTAGAGTCAGCATTGAGGCGGGATCAACATTTGGTTGGCATAAGATTGTTGGAAGCAAGGGAAAAACCATTGGCATTGATCGATTTGGAGCAAGTGCTCCAGCAGGGAAAATATACAAGGAGTATGGCATAACCAAGGAAGCTGTTATTTCTGCTGCCAAAGAAGTTTCATAG
Protein sequence

>MS.gene74217.t1

MASSSSLHLSQAFLARAVYLNPEIRASSLSSTSSLSFPALKSSSSCKPAATSSAGSRRRTTPAIRATAVETLDKTTEASLVEKSVNTIRFLAIDAVEKANSGHPGLPMGCAPMGHILYDEVMRYNPKNPSWFNRDRFVLSAGHGCMLQYALLHLAGYDSVKEEDLKQFRQWGSKTPGHPENFETYGIEVTTGPLGQGIANGVGLALAEKHLAARFNKPDSEIVDHYTYVILGDGCQMEGISNEACSLAGHWGLGKLIAFYDDNHISIDGDTEIAFTENVDQRFEALGWHVIWVKNGNTGYDEIRAAIKEAKAVTDKPTLIKVTTTIGFGSPNKANSYSVHGAALGSKEVDATRQNLGWPYEPFHVPEDVKKHWSRHTPEGAALEAEWNAKFAEYEKKYKEEAAELKAIINRELPADWEKALPTYTPETPADATRNLSQQNLNALAKVLPGLIGGSADLASSNMTLLKSFGDFQKATPEERNVRFGVREHAMGAICNGIALHSPGFIPYCATFFVFTDYMRAAIRISALCEAGVIYVMTHDSIGLGEDGPTHQPIEHLASFRAMPNVLMLRPADGNETAGSYRVAVLNQKRPSILALSRQKLPNLPGTSIEGVEKGGYTISDNSSGNKPDVILIGTGSELEIAAAAADDLRKEGKTVRVVSFVSWELFDDQSDEYKESVLPASVTARVSIEAGSTFGWHKIVGSKGKTIGIDRFGASAPAGKIYKEYGITKEAVISAAKEVS