AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25675


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25675.t1 MTR_7g107680 86.813 182 19 2 1 177 1 182 2.76e-100 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25675.t1 AT4G03150 60.000 130 50 1 44 171 52 181 5.78e-52 164

Find 0 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTTTCCTTATAAGAAA+TGG + 51705:6594-6613 None:intergenic 20.0%
!!! TTTTTCCATTCACAATTATT+AGG - 51705:6397-6416 MS.gene25675:intron 20.0%
! ATTGGAAACCATTTCTTATA+AGG - 51705:6583-6602 MS.gene25675:CDS 25.0%
! GAATTGGTTACATATTGTTA+AGG - 51705:6159-6178 MS.gene25675:intron 25.0%
! TGATTCCTAATAATTGTGAA+TGG + 51705:6405-6424 None:intergenic 25.0%
!! CTTATAAGGAAAGTTTTCAT+GGG - 51705:6597-6616 MS.gene25675:CDS 25.0%
!! TCTTATAAGGAAAGTTTTCA+TGG - 51705:6596-6615 MS.gene25675:CDS 25.0%
!! TTAGGGTTACTTTACATAAA+TGG - 51705:6432-6451 MS.gene25675:CDS 25.0%
!! TTATAAGGAAAGTTTTCATG+GGG - 51705:6598-6617 MS.gene25675:CDS 25.0%
AAATGGATGAGAATGTGAAT+GGG + 51705:5988-6007 None:intergenic 30.0%
ACAATTATTAGGAATCAGAG+TGG - 51705:6408-6427 MS.gene25675:intron 30.0%
CAAAGAATTGTAGTAGGATT+TGG + 51705:6513-6532 None:intergenic 30.0%
CCTACTACAATTCTTTGATA+CGG - 51705:6516-6535 MS.gene25675:CDS 30.0%
TGAGATGTTCATGTTAGAAT+TGG - 51705:6143-6162 MS.gene25675:intron 30.0%
! AGCTGACATTTTGTTGAATA+TGG - 51705:6553-6572 MS.gene25675:CDS 30.0%
! GGTTACTTTACATAAATGGT+TGG - 51705:6436-6455 MS.gene25675:CDS 30.0%
!! ACGTGTTTCTAAAATTTGAG+AGG + 51705:6108-6127 None:intergenic 30.0%
ATGAAGATATAAATGAGCGC+AGG + 51705:6367-6386 None:intergenic 35.0%
CCGTATCAAAGAATTGTAGT+AGG + 51705:6519-6538 None:intergenic 35.0%
GAAATGGATGAGAATGTGAA+TGG + 51705:5989-6008 None:intergenic 35.0%
! AAGATGAAGAAGAAGCGTAT+TGG + 51705:5932-5951 None:intergenic 35.0%
!! AATGAAAAGAGTACCTACCT+GGG + 51705:6081-6100 None:intergenic 35.0%
AAAGTAATCACATGTGTCCG+TGG - 51705:6288-6307 MS.gene25675:intron 40.0%
ATCTCCACATTTGTTTCCTC+AGG + 51705:6476-6495 None:intergenic 40.0%
ATTAGGAATCAGAGTGGCTT+AGG - 51705:6414-6433 MS.gene25675:intron 40.0%
CTAGTGATTTCAACACCGAA+AGG + 51705:6314-6333 None:intergenic 40.0%
GTGAGTGAATGTTACTGTCA+CGG + 51705:5907-5926 None:intergenic 40.0%
GTTGAAATCACTAGGTGGTT+AGG - 51705:6320-6339 MS.gene25675:intron 40.0%
GTTGAATATGGCACGTGAAT+TGG - 51705:6565-6584 MS.gene25675:CDS 40.0%
TTAGGAATCAGAGTGGCTTA+GGG - 51705:6415-6434 MS.gene25675:intron 40.0%
! ATGAAGAAGAAGCGTATTGG+TGG + 51705:5929-5948 None:intergenic 40.0%
! CGAGTAGTGTGGACAATTTT+TGG + 51705:5963-5982 None:intergenic 40.0%
!! CTTTCGGTGTTGAAATCACT+AGG - 51705:6312-6331 MS.gene25675:intron 40.0%
!! GAATGAAAAGAGTACCTACC+TGG + 51705:6082-6101 None:intergenic 40.0%
!!! GTGGACAATTTTTGGTGAAG+AGG + 51705:5955-5974 None:intergenic 40.0%
AGGAGGAAGATGGTGTGAAT+GGG + 51705:5884-5903 None:intergenic 45.0%
AGTGAATGTTACTGTCACGG+AGG + 51705:5904-5923 None:intergenic 45.0%
ATTTCAACACCGAAAGGCCA+CGG + 51705:6308-6327 None:intergenic 45.0%
CTGTCCTGAGGAAACAAATG+TGG - 51705:6469-6488 MS.gene25675:CDS 45.0%
GAACACGAAGGTCTTCAACT+TGG + 51705:6028-6047 None:intergenic 45.0%
GGATGATGAATACTGTCCTG+AGG - 51705:6457-6476 MS.gene25675:CDS 45.0%
GTCTTCAACTTGGTCGTCAT+CGG + 51705:6018-6037 None:intergenic 45.0%
TCTTCAACTTGGTCGTCATC+GGG + 51705:6017-6036 None:intergenic 45.0%
TGGTTGGGAGTAACCAATGA+GGG + 51705:6052-6071 None:intergenic 45.0%
TTGGTTGGGAGTAACCAATG+AGG + 51705:6053-6072 None:intergenic 45.0%
! TAGTTAGCTTAGACCGACGA+AGG - 51705:6224-6243 MS.gene25675:intron 45.0%
!! TCGGTGTTGAAATCACTAGG+TGG - 51705:6315-6334 MS.gene25675:intron 45.0%
AGCCGCATTTGCAGATGTGT+AGG + 51705:6624-6643 None:intergenic 50.0%
CTGTAAACCTAATCGCGCAG+CGG - 51705:6642-6661 MS.gene25675:CDS 50.0%
GAATGTTACTGTCACGGAGG+AGG + 51705:5901-5920 None:intergenic 50.0%
GAGGAGGAAGATGGTGTGAA+TGG + 51705:5885-5904 None:intergenic 50.0%
GGCCTACACATCTGCAAATG+CGG - 51705:6619-6638 MS.gene25675:CDS 50.0%
GGTTGGGAGTAACCAATGAG+GGG + 51705:6051-6070 None:intergenic 50.0%
GTGAATGGGTACGAGTAGTG+TGG + 51705:5974-5993 None:intergenic 50.0%
GTGTGAATGGGATGATGCTG+CGG + 51705:5872-5891 None:intergenic 50.0%
GTTGGGAGTAACCAATGAGG+GGG + 51705:6050-6069 None:intergenic 50.0%
TGTGAATGGGATGATGCTGC+GGG + 51705:5871-5890 None:intergenic 50.0%
!! GAAAAGAGTACCTACCTGGG+AGG + 51705:6078-6097 None:intergenic 50.0%
!! TCGTCATCGGGTTCTGGAAA+TGG + 51705:6005-6024 None:intergenic 50.0%
ACCAATGAGGGGGAACACGA+AGG + 51705:6040-6059 None:intergenic 55.0%
ACCTTCGTGTTCCCCCTCAT+TGG - 51705:6036-6055 MS.gene25675:CDS 55.0%
ACTGTCACGGAGGAGGAAGA+TGG + 51705:5894-5913 None:intergenic 55.0%
ACTTGGTCGTCATCGGGTTC+TGG + 51705:6011-6030 None:intergenic 55.0%
CACATGTGTCCGTGGCCTTT+CGG - 51705:6296-6315 MS.gene25675:intron 55.0%
CGACGAAGGACACGTCAACA+CGG - 51705:6238-6257 MS.gene25675:intron 55.0%
GCTGCGGGGAGATAGAATGA+TGG + 51705:5856-5875 None:intergenic 55.0%
GTGAATGGGATGATGCTGCG+GGG + 51705:5870-5889 None:intergenic 55.0%
GTTCAATCCGCTGCGCGATT+AGG + 51705:6652-6671 None:intergenic 55.0%
! AGTACCTACCTGGGAGGCAT+TGG + 51705:6072-6091 None:intergenic 55.0%
! CGTGTTGACGTGTCCTTCGT+CGG + 51705:6240-6259 None:intergenic 55.0%
!! CTACCTGGGAGGCATTGGTT+GGG + 51705:6067-6086 None:intergenic 55.0%
ACTCCCAACCAATGCCTCCC+AGG - 51705:6061-6080 MS.gene25675:CDS 60.0%
CCAACCAATGCCTCCCAGGT+AGG - 51705:6065-6084 MS.gene25675:CDS 60.0%
!! CCTACCTGGGAGGCATTGGT+TGG + 51705:6068-6087 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
51705 gene 5822 6691 5822 ID=MS.gene25675
51705 mRNA 5822 6691 5822 ID=MS.gene25675.t1;Parent=MS.gene25675
51705 exon 6431 6691 6431 ID=MS.gene25675.t1.exon1;Parent=MS.gene25675.t1
51705 CDS 6431 6691 6431 ID=cds.MS.gene25675.t1;Parent=MS.gene25675.t1
51705 exon 5822 6094 5822 ID=MS.gene25675.t1.exon2;Parent=MS.gene25675.t1
51705 CDS 5822 6094 5822 ID=cds.MS.gene25675.t1;Parent=MS.gene25675.t1
Gene Sequence

>MS.gene25675

ATGTCAAGCTTGAAATTGAATCTAGTTCATACCATCATTCTATCTCCCCGCAGCATCATCCCATTCACACCATCTTCCTCCTCCGTGACAGTAACATTCACTCACCACCAATACGCTTCTTCTTCATCTTCCTCTTCACCAAAAATTGTCCACACTACTCGTACCCATTCACATTCTCATCCATTTCCAGAACCCGATGACGACCAAGTTGAAGACCTTCGTGTTCCCCCTCATTGGTTACTCCCAACCAATGCCTCCCAGGTAGGTACTCTTTTCATTCATTCCTCTCAAATTTTAGAAACACGTAGTTGTACTTGTAGCTGAGATGTTCATGTTAGAATTGGTTACATATTGTTAAGGTAGTTACAATTGAGTTATGATTAGTTAAAAGTTAGTTAGAATTAGTTAGCTTAGACCGACGAAGGACACGTCAACACGGTAATAATTTGAGAAAACAGCATTGTTGAAAGTAATCACATGTGTCCGTGGCCTTTCGGTGTTGAAATCACTAGGTGGTTAGGATCACTATAAAAAAAATGTATCCTGCGCTCATTTATATCTTCATTAATTGTTTTTTTTTCCATTCACAATTATTAGGAATCAGAGTGGCTTAGGGTTACTTTACATAAATGGTTGGATGATGAATACTGTCCTGAGGAAACAAATGTGGAGATAAGCAGAGTTGCTGCCAAATCCTACTACAATTCTTTGATACGGAAACAAGCTGATTTAGCTGACATTTTGTTGAATATGGCACGTGAATTGGAAACCATTTCTTATAAGGAAAGTTTTCATGGGGCCTACACATCTGCAAATGCGGCTGTAAACCTAATCGCGCAGCGGATTGAACAATTCTGCCACCCAAATTGA

Protein sequence

>MS.gene25675.t1

MSSLKLNLVHTIILSPRSIIPFTPSSSSVTVTFTHHQYASSSSSSSPKIVHTTRTHSHSHPFPEPDDDQVEDLRVPPHWLLPTNASQESEWLRVTLHKWLDDEYCPEETNVEISRVAAKSYYNSLIRKQADLADILLNMARELETISYKESFHGAYTSANAAVNLIAQRIEQFCHPN