AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23018


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23018.t1 MTR_7g107680 86.813 182 19 2 1 177 1 182 2.76e-100 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23018.t1 AT4G03150 60.000 130 50 1 44 171 52 181 5.78e-52 164

Find 50 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGGGTTACTTTACATAAA+TGG 0.225005 7.2:+6850644 MS.gene23018:CDS
ATTGGAAACCATTTCTTATA+AGG 0.243835 7.2:+6850795 MS.gene23018:CDS
TGGAGCTATGAAATTCAATT+TGG 0.253108 7.2:-6850895 None:intergenic
GTTCAATCCGCTGCGCGATT+AGG 0.295554 7.2:-6850861 None:intergenic
ACTTGGTCGTCATCGGGTTC+TGG 0.304693 7.2:-6850215 None:intergenic
CAAAGAATTGTAGTAGGATT+TGG 0.316745 7.2:-6850722 None:intergenic
ATTAGGAATCAGAGTGGCTT+AGG 0.341814 7.2:+6850626 MS.gene23018:intron
AAAACTTTCCTTATAAGAAA+TGG 0.350129 7.2:-6850803 None:intergenic
ATCTCCACATTTGTTTCCTC+AGG 0.391134 7.2:-6850685 None:intergenic
GTTGAATATGGCACGTGAAT+TGG 0.399859 7.2:+6850777 MS.gene23018:CDS
GTCTTCAACTTGGTCGTCAT+CGG 0.400136 7.2:-6850222 None:intergenic
TCGTCATCGGGTTCTGGAAA+TGG 0.412464 7.2:-6850209 None:intergenic
GAGGAGGAAGATGGTGTGAA+TGG 0.458635 7.2:-6850089 None:intergenic
TCTTCAACTTGGTCGTCATC+GGG 0.460270 7.2:-6850221 None:intergenic
CCGTATCAAAGAATTGTAGT+AGG 0.472999 7.2:-6850728 None:intergenic
CTACCTGGGAGGCATTGGTT+GGG 0.478796 7.2:-6850271 None:intergenic
AGTACCTACCTGGGAGGCAT+TGG 0.485202 7.2:-6850276 None:intergenic
AAGATGAAGAAGAAGCGTAT+TGG 0.486292 7.2:-6850136 None:intergenic
GGAGCTATGAAATTCAATTT+GGG 0.488254 7.2:-6850894 None:intergenic
GGTTACTTTACATAAATGGT+TGG 0.503773 7.2:+6850648 MS.gene23018:CDS
GAAATGGATGAGAATGTGAA+TGG 0.509383 7.2:-6850193 None:intergenic
AGGAGGAAGATGGTGTGAAT+GGG 0.510323 7.2:-6850088 None:intergenic
CCTACTACAATTCTTTGATA+CGG 0.511408 7.2:+6850728 MS.gene23018:CDS
TTAGGAATCAGAGTGGCTTA+GGG 0.523126 7.2:+6850627 MS.gene23018:intron
ATGAAGAAGAAGCGTATTGG+TGG 0.538072 7.2:-6850133 None:intergenic
ACTCCCAACCAATGCCTCCC+AGG 0.538117 7.2:+6850268 MS.gene23018:CDS
CCTACCTGGGAGGCATTGGT+TGG 0.555347 7.2:-6850272 None:intergenic
TGTGAATGGGATGATGCTGC+GGG 0.566309 7.2:-6850075 None:intergenic
GAACACGAAGGTCTTCAACT+TGG 0.573094 7.2:-6850232 None:intergenic
GAATGTTACTGTCACGGAGG+AGG 0.577024 7.2:-6850105 None:intergenic
GTGTGAATGGGATGATGCTG+CGG 0.578527 7.2:-6850076 None:intergenic
ACTGTCACGGAGGAGGAAGA+TGG 0.589440 7.2:-6850098 None:intergenic
TGGTTGGGAGTAACCAATGA+GGG 0.597474 7.2:-6850256 None:intergenic
AGCCGCATTTGCAGATGTGT+AGG 0.600800 7.2:-6850833 None:intergenic
CTGTCCTGAGGAAACAAATG+TGG 0.608003 7.2:+6850681 MS.gene23018:CDS
GCTATGAAATTCAATTTGGG+TGG 0.608458 7.2:-6850891 None:intergenic
GGCCTACACATCTGCAAATG+CGG 0.641836 7.2:+6850831 MS.gene23018:CDS
GTGAATGGGTACGAGTAGTG+TGG 0.653357 7.2:-6850178 None:intergenic
AAATGGATGAGAATGTGAAT+GGG 0.656050 7.2:-6850192 None:intergenic
TTGGTTGGGAGTAACCAATG+AGG 0.663293 7.2:-6850257 None:intergenic
ACAATTATTAGGAATCAGAG+TGG 0.674841 7.2:+6850620 MS.gene23018:intron
CTGTAAACCTAATCGCGCAG+CGG 0.684704 7.2:+6850854 MS.gene23018:CDS
GTGAGTGAATGTTACTGTCA+CGG 0.689434 7.2:-6850111 None:intergenic
GCTGCGGGGAGATAGAATGA+TGG 0.699964 7.2:-6850060 None:intergenic
GTTGGGAGTAACCAATGAGG+GGG 0.704622 7.2:-6850254 None:intergenic
GGTTGGGAGTAACCAATGAG+GGG 0.709296 7.2:-6850255 None:intergenic
GGATGATGAATACTGTCCTG+AGG 0.725475 7.2:+6850669 MS.gene23018:CDS
GTGAATGGGATGATGCTGCG+GGG 0.726743 7.2:-6850074 None:intergenic
GAAAAGAGTACCTACCTGGG+AGG 0.727204 7.2:-6850282 None:intergenic
AGTGAATGTTACTGTCACGG+AGG 0.780859 7.2:-6850108 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTTTCCTTATAAGAAA+TGG - chr7.2:6850806-6850825 None:intergenic 20.0%
!!! CTATTTTCTCAAATTATTAC+GGG - chr7.2:6850468-6850487 None:intergenic 20.0%
!!! GCTATTTTCTCAAATTATTA+CGG - chr7.2:6850469-6850488 None:intergenic 20.0%
!!! TTTTTCCATTCACAATTATT+AGG + chr7.2:6850609-6850628 MS.gene23018:intron 20.0%
! ATTGGAAACCATTTCTTATA+AGG + chr7.2:6850795-6850814 MS.gene23018:CDS 25.0%
! GAATTGGTTACATATTGTTA+AGG + chr7.2:6850366-6850385 MS.gene23018:intron 25.0%
! TGATTCCTAATAATTGTGAA+TGG - chr7.2:6850617-6850636 None:intergenic 25.0%
!! CTTATAAGGAAAGTTTTCAT+GGG + chr7.2:6850809-6850828 MS.gene23018:CDS 25.0%
!! TCTTATAAGGAAAGTTTTCA+TGG + chr7.2:6850808-6850827 MS.gene23018:CDS 25.0%
!! TTAGGGTTACTTTACATAAA+TGG + chr7.2:6850644-6850663 MS.gene23018:CDS 25.0%
!! TTATAAGGAAAGTTTTCATG+GGG + chr7.2:6850810-6850829 MS.gene23018:CDS 25.0%
AAATGGATGAGAATGTGAAT+GGG - chr7.2:6850195-6850214 None:intergenic 30.0%
ACAATTATTAGGAATCAGAG+TGG + chr7.2:6850620-6850639 MS.gene23018:intron 30.0%
CAAAGAATTGTAGTAGGATT+TGG - chr7.2:6850725-6850744 None:intergenic 30.0%
CCTACTACAATTCTTTGATA+CGG + chr7.2:6850728-6850747 MS.gene23018:CDS 30.0%
TGAGATGTTCATGTTAGAAT+TGG + chr7.2:6850350-6850369 MS.gene23018:intron 30.0%
! AGCTGACATTTTGTTGAATA+TGG + chr7.2:6850765-6850784 MS.gene23018:CDS 30.0%
! GGTTACTTTACATAAATGGT+TGG + chr7.2:6850648-6850667 MS.gene23018:CDS 30.0%
!! ACGTGTTTCTAAAATTTGAG+AGG - chr7.2:6850315-6850334 None:intergenic 30.0%
ATGAAGATATAAATGAGCGC+AGG - chr7.2:6850579-6850598 None:intergenic 35.0%
CCGTATCAAAGAATTGTAGT+AGG - chr7.2:6850731-6850750 None:intergenic 35.0%
GAAATGGATGAGAATGTGAA+TGG - chr7.2:6850196-6850215 None:intergenic 35.0%
! AAGATGAAGAAGAAGCGTAT+TGG - chr7.2:6850139-6850158 None:intergenic 35.0%
!! AATGAAAAGAGTACCTACCT+GGG - chr7.2:6850288-6850307 None:intergenic 35.0%
ATCTCCACATTTGTTTCCTC+AGG - chr7.2:6850688-6850707 None:intergenic 40.0%
ATTAGGAATCAGAGTGGCTT+AGG + chr7.2:6850626-6850645 MS.gene23018:intron 40.0%
ATTTCAACACCGAAAGGACA+CGG - chr7.2:6850521-6850540 None:intergenic 40.0%
CTAGTGATTTCAACACCGAA+AGG - chr7.2:6850527-6850546 None:intergenic 40.0%
GTGAGTGAATGTTACTGTCA+CGG - chr7.2:6850114-6850133 None:intergenic 40.0%
GTTGAAATCACTAGGTGGTT+AGG + chr7.2:6850533-6850552 MS.gene23018:intron 40.0%
GTTGAATATGGCACGTGAAT+TGG + chr7.2:6850777-6850796 MS.gene23018:CDS 40.0%
TTAGGAATCAGAGTGGCTTA+GGG + chr7.2:6850627-6850646 MS.gene23018:intron 40.0%
! ATGAAGAAGAAGCGTATTGG+TGG - chr7.2:6850136-6850155 None:intergenic 40.0%
! CGAGTAGTGTGGACAATTTT+TGG - chr7.2:6850170-6850189 None:intergenic 40.0%
!! CTTTCGGTGTTGAAATCACT+AGG + chr7.2:6850525-6850544 MS.gene23018:intron 40.0%
!! GAATGAAAAGAGTACCTACC+TGG - chr7.2:6850289-6850308 None:intergenic 40.0%
!!! GTGGACAATTTTTGGTGAAG+AGG - chr7.2:6850162-6850181 None:intergenic 40.0%
AGGAGGAAGATGGTGTGAAT+GGG - chr7.2:6850091-6850110 None:intergenic 45.0%
AGTGAATGTTACTGTCACGG+AGG - chr7.2:6850111-6850130 None:intergenic 45.0%
CTGTCCTGAGGAAACAAATG+TGG + chr7.2:6850681-6850700 MS.gene23018:CDS 45.0%
GAACACGAAGGTCTTCAACT+TGG - chr7.2:6850235-6850254 None:intergenic 45.0%
GGATGATGAATACTGTCCTG+AGG + chr7.2:6850669-6850688 MS.gene23018:CDS 45.0%
GTCTTCAACTTGGTCGTCAT+CGG - chr7.2:6850225-6850244 None:intergenic 45.0%
TCTTCAACTTGGTCGTCATC+GGG - chr7.2:6850224-6850243 None:intergenic 45.0%
TGGTTGGGAGTAACCAATGA+GGG - chr7.2:6850259-6850278 None:intergenic 45.0%
TTGGTTGGGAGTAACCAATG+AGG - chr7.2:6850260-6850279 None:intergenic 45.0%
! TAGTTAGCTTAGACCGACGA+AGG + chr7.2:6850431-6850450 MS.gene23018:intron 45.0%
!! TCGGTGTTGAAATCACTAGG+TGG + chr7.2:6850528-6850547 MS.gene23018:intron 45.0%
AGCCGCATTTGCAGATGTGT+AGG - chr7.2:6850836-6850855 None:intergenic 50.0%
CACATGTGTCCGTGTCCTTT+CGG + chr7.2:6850509-6850528 MS.gene23018:intron 50.0%
CTGTAAACCTAATCGCGCAG+CGG + chr7.2:6850854-6850873 MS.gene23018:CDS 50.0%
GAATGTTACTGTCACGGAGG+AGG - chr7.2:6850108-6850127 None:intergenic 50.0%
GAGGAGGAAGATGGTGTGAA+TGG - chr7.2:6850092-6850111 None:intergenic 50.0%
GGCCTACACATCTGCAAATG+CGG + chr7.2:6850831-6850850 MS.gene23018:CDS 50.0%
GGTTGGGAGTAACCAATGAG+GGG - chr7.2:6850258-6850277 None:intergenic 50.0%
GTGAATGGGTACGAGTAGTG+TGG - chr7.2:6850181-6850200 None:intergenic 50.0%
GTGTGAATGGGATGATGCTG+CGG - chr7.2:6850079-6850098 None:intergenic 50.0%
GTTGGGAGTAACCAATGAGG+GGG - chr7.2:6850257-6850276 None:intergenic 50.0%
TGTGAATGGGATGATGCTGC+GGG - chr7.2:6850078-6850097 None:intergenic 50.0%
!! GAAAAGAGTACCTACCTGGG+AGG - chr7.2:6850285-6850304 None:intergenic 50.0%
!! TCGTCATCGGGTTCTGGAAA+TGG - chr7.2:6850212-6850231 None:intergenic 50.0%
ACCAATGAGGGGGAACACGA+AGG - chr7.2:6850247-6850266 None:intergenic 55.0%
ACCTTCGTGTTCCCCCTCAT+TGG + chr7.2:6850243-6850262 MS.gene23018:CDS 55.0%
ACTGTCACGGAGGAGGAAGA+TGG - chr7.2:6850101-6850120 None:intergenic 55.0%
ACTTGGTCGTCATCGGGTTC+TGG - chr7.2:6850218-6850237 None:intergenic 55.0%
GCTGCGGGGAGATAGAATGA+TGG - chr7.2:6850063-6850082 None:intergenic 55.0%
GTGAATGGGATGATGCTGCG+GGG - chr7.2:6850077-6850096 None:intergenic 55.0%
GTTCAATCCGCTGCGCGATT+AGG - chr7.2:6850864-6850883 None:intergenic 55.0%
! AGTACCTACCTGGGAGGCAT+TGG - chr7.2:6850279-6850298 None:intergenic 55.0%
!! CTACCTGGGAGGCATTGGTT+GGG - chr7.2:6850274-6850293 None:intergenic 55.0%
!! GGTGTTGACGTGTCCTTCGT+CGG - chr7.2:6850447-6850466 None:intergenic 55.0%
ACTCCCAACCAATGCCTCCC+AGG + chr7.2:6850268-6850287 MS.gene23018:CDS 60.0%
CCAACCAATGCCTCCCAGGT+AGG + chr7.2:6850272-6850291 MS.gene23018:intron 60.0%
!! CCTACCTGGGAGGCATTGGT+TGG - chr7.2:6850275-6850294 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 6850029 6850903 6850029 ID=MS.gene23018
chr7.2 mRNA 6850029 6850903 6850029 ID=MS.gene23018.t1;Parent=MS.gene23018
chr7.2 exon 6850029 6850289 6850029 ID=MS.gene23018.t1.exon1;Parent=MS.gene23018.t1
chr7.2 CDS 6850029 6850289 6850029 ID=cds.MS.gene23018.t1;Parent=MS.gene23018.t1
chr7.2 exon 6850631 6850903 6850631 ID=MS.gene23018.t1.exon2;Parent=MS.gene23018.t1
chr7.2 CDS 6850631 6850903 6850631 ID=cds.MS.gene23018.t1;Parent=MS.gene23018.t1
Gene Sequence

>MS.gene23018

ATGTCAAGCTTGAAATTGAATCTAGTTCATACCATCATTCTATCTCCCCGCAGCATCATCCCATTCACACCATCTTCCTCCTCCGTGACAGTAACATTCACTCACCACCAATACGCTTCTTCTTCATCTTCCTCTTCACCAAAAATTGTCCACACTACTCGTACCCATTCACATTCTCATCCATTTCCAGAACCCGATGACGACCAAGTTGAAGACCTTCGTGTTCCCCCTCATTGGTTACTCCCAACCAATGCCTCCCAGGTAGGTACTCTTTTCATTCATTCCTCTCAAATTTTAGAAACACGTAGTTGTACTTGTAGCTGAGATGTTCATGTTAGAATTGGTTACATATTGTTAAGGTAGTTACAATTGAGTTATGATTAGTTAAAAGTTAGTTAGAATTAGTTAGCTTAGACCGACGAAGGACACGTCAACACCCGTAATAATTTGAGAAAATAGCATTGTTGAATTGAAAGTAATCACATGTGTCCGTGTCCTTTCGGTGTTGAAATCACTAGGTGGTTAGGATCACTATAAAAAAATGTATCCTGCGCTCATTTATATCTTCATTAATTGTTTTTTTTTCCATTCACAATTATTAGGAATCAGAGTGGCTTAGGGTTACTTTACATAAATGGTTGGATGATGAATACTGTCCTGAGGAAACAAATGTGGAGATAAGCAGAGTTGCTGCCAAATCCTACTACAATTCTTTGATACGGAAACAAGCTGATTTAGCTGACATTTTGTTGAATATGGCACGTGAATTGGAAACCATTTCTTATAAGGAAAGTTTTCATGGGGCCTACACATCTGCAAATGCGGCTGTAAACCTAATCGCGCAGCGGATTGAACAATTCTGCCACCCAAATTGA

Protein sequence

>MS.gene23018.t1

MSSLKLNLVHTIILSPRSIIPFTPSSSSVTVTFTHHQYASSSSSSSPKIVHTTRTHSHSHPFPEPDDDQVEDLRVPPHWLLPTNASQESEWLRVTLHKWLDDEYCPEETNVEISRVAAKSYYNSLIRKQADLADILLNMARELETISYKESFHGAYTSANAAVNLIAQRIEQFCHPN