Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20225.t1 | XP_013447222.1 | 94.6 | 147 | 8 | 0 | 1 | 147 | 1 | 147 | 5.90E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20225.t1 | A0A072TUP6 | 94.6 | 147 | 8 | 0 | 1 | 147 | 1 | 147 | 4.3e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene20225 | 0.860495 | 1.98E-63 | -1.69E-46 |
| MS.gene049171 | MS.gene20225 | 0.813106 | 3.03E-51 | -1.69E-46 |
| MS.gene049351 | MS.gene20225 | 0.81296 | 3.26E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene20225 | 0.83814 | 3.43E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene20225 | 0.849609 | 2.90E-60 | -1.69E-46 |
| MS.gene049661 | MS.gene20225 | 0.806229 | 9.10E-50 | -1.69E-46 |
| MS.gene049662 | MS.gene20225 | 0.803604 | 3.22E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene20225 | 0.870947 | 9.93E-67 | -1.69E-46 |
| MS.gene050076 | MS.gene20225 | 0.819116 | 1.37E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene20225 | 0.816565 | 5.18E-52 | -1.69E-46 |
| MS.gene050432 | MS.gene20225 | 0.838011 | 3.71E-57 | -1.69E-46 |
| MS.gene050529 | MS.gene20225 | 0.80802 | 3.80E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene20225 | 0.840044 | 1.10E-57 | -1.69E-46 |
| MS.gene050625 | MS.gene20225 | 0.848493 | 5.92E-60 | -1.69E-46 |
| MS.gene050670 | MS.gene20225 | 0.873526 | 1.37E-67 | -1.69E-46 |
| MS.gene050747 | MS.gene20225 | 0.837737 | 4.36E-57 | -1.69E-46 |
| MS.gene050770 | MS.gene20225 | 0.801598 | 8.35E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene20225 | 0.829807 | 4.19E-55 | -1.69E-46 |
| MS.gene050812 | MS.gene20225 | 0.838093 | 3.53E-57 | -1.69E-46 |
| MS.gene05081 | MS.gene20225 | 0.833587 | 4.90E-56 | -1.69E-46 |
| MS.gene050950 | MS.gene20225 | 0.8235 | 1.34E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene20225 | 0.821003 | 5.09E-53 | -1.69E-46 |
| MS.gene05105 | MS.gene20225 | 0.866025 | 3.86E-65 | -1.69E-46 |
| MS.gene051387 | MS.gene20225 | 0.861714 | 8.45E-64 | -1.69E-46 |
| MS.gene051525 | MS.gene20225 | 0.850201 | 1.98E-60 | -1.69E-46 |
| MS.gene051582 | MS.gene20225 | 0.872337 | 3.44E-67 | -1.69E-46 |
| MS.gene051666 | MS.gene20225 | 0.856687 | 2.72E-62 | -1.69E-46 |
| MS.gene051692 | MS.gene20225 | 0.84641 | 2.21E-59 | -1.69E-46 |
| MS.gene051838 | MS.gene20225 | 0.819259 | 1.28E-52 | -1.69E-46 |
| MS.gene051858 | MS.gene20225 | 0.803123 | 4.05E-49 | -1.69E-46 |
| MS.gene051877 | MS.gene20225 | 0.804841 | 1.78E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene20225 | -0.81417 | 1.77E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene20225 | 0.825943 | 3.56E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene20225 | 0.839246 | 1.78E-57 | -1.69E-46 |
| MS.gene052310 | MS.gene20225 | 0.85796 | 1.14E-62 | -1.69E-46 |
| MS.gene052322 | MS.gene20225 | 0.851904 | 6.55E-61 | -1.69E-46 |
| MS.gene052340 | MS.gene20225 | 0.827102 | 1.88E-54 | -1.69E-46 |
| MS.gene052379 | MS.gene20225 | 0.81509 | 1.10E-51 | -1.69E-46 |
| MS.gene052414 | MS.gene20225 | 0.807665 | 4.52E-50 | -1.69E-46 |
| MS.gene052421 | MS.gene20225 | 0.878917 | 1.90E-69 | -1.69E-46 |
| MS.gene052481 | MS.gene20225 | 0.896038 | 5.41E-76 | -1.69E-46 |
| MS.gene052484 | MS.gene20225 | 0.864415 | 1.24E-64 | -1.69E-46 |
| MS.gene052485 | MS.gene20225 | 0.858316 | 8.96E-63 | -1.69E-46 |
| MS.gene052501 | MS.gene20225 | 0.86174 | 8.30E-64 | -1.69E-46 |
| MS.gene052503 | MS.gene20225 | 0.815821 | 7.59E-52 | -1.69E-46 |
| MS.gene052505 | MS.gene20225 | 0.851552 | 8.24E-61 | -1.69E-46 |
| MS.gene052615 | MS.gene20225 | 0.839153 | 1.88E-57 | -1.69E-46 |
| MS.gene052823 | MS.gene20225 | 0.803779 | 2.96E-49 | -1.69E-46 |
| MS.gene052825 | MS.gene20225 | 0.805043 | 1.62E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene20225 | 0.868031 | 8.83E-66 | -1.69E-46 |
| MS.gene053103 | MS.gene20225 | 0.881114 | 3.14E-70 | -1.69E-46 |
| MS.gene053104 | MS.gene20225 | 0.84407 | 9.49E-59 | -1.69E-46 |
| MS.gene053107 | MS.gene20225 | 0.815794 | 7.70E-52 | -1.69E-46 |
| MS.gene053207 | MS.gene20225 | 0.836645 | 8.29E-57 | -1.69E-46 |
| MS.gene053209 | MS.gene20225 | 0.84433 | 8.09E-59 | -1.69E-46 |
| MS.gene053213 | MS.gene20225 | 0.822358 | 2.47E-53 | -1.69E-46 |
| MS.gene053286 | MS.gene20225 | 0.80821 | 3.46E-50 | -1.69E-46 |
| MS.gene053322 | MS.gene20225 | 0.863475 | 2.42E-64 | -1.69E-46 |
| MS.gene053449 | MS.gene20225 | 0.812557 | 3.99E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene20225 | 0.810728 | 9.98E-51 | -1.69E-46 |
| MS.gene053579 | MS.gene20225 | 0.804091 | 2.55E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene20225 | 0.819691 | 1.02E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene20225 | 0.810404 | 1.17E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene20225 | 0.803941 | 2.74E-49 | -1.69E-46 |
| MS.gene054408 | MS.gene20225 | 0.838176 | 3.36E-57 | -1.69E-46 |
| MS.gene054600 | MS.gene20225 | 0.822886 | 1.86E-53 | -1.69E-46 |
| MS.gene054719 | MS.gene20225 | 0.800327 | 1.52E-48 | -1.69E-46 |
| MS.gene054802 | MS.gene20225 | 0.801463 | 8.90E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene20225 | 0.802653 | 5.06E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene20225 | 0.829936 | 3.90E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene20225 | 0.849519 | 3.07E-60 | -1.69E-46 |
| MS.gene055116 | MS.gene20225 | 0.835281 | 1.84E-56 | -1.69E-46 |
| MS.gene055206 | MS.gene20225 | 0.84581 | 3.22E-59 | -1.69E-46 |
| MS.gene055224 | MS.gene20225 | 0.802331 | 5.90E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene20225 | 0.814934 | 1.20E-51 | -1.69E-46 |
| MS.gene055289 | MS.gene20225 | 0.838625 | 2.57E-57 | -1.69E-46 |
| MS.gene055412 | MS.gene20225 | 0.881871 | 1.67E-70 | -1.69E-46 |
| MS.gene055428 | MS.gene20225 | 0.811366 | 7.26E-51 | -1.69E-46 |
| MS.gene055499 | MS.gene20225 | 0.802114 | 6.54E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene20225 | 0.819369 | 1.20E-52 | -1.69E-46 |
| MS.gene055647 | MS.gene20225 | 0.809134 | 2.20E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene20225 | 0.819595 | 1.07E-52 | -1.69E-46 |
| MS.gene05592 | MS.gene20225 | 0.834016 | 3.82E-56 | -1.69E-46 |
| MS.gene055941 | MS.gene20225 | 0.82436 | 8.42E-54 | -1.69E-46 |
| MS.gene056191 | MS.gene20225 | 0.811549 | 6.62E-51 | -1.69E-46 |
| MS.gene056374 | MS.gene20225 | 0.812087 | 5.06E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene20225 | 0.851012 | 1.17E-60 | -1.69E-46 |
| MS.gene056497 | MS.gene20225 | 0.811532 | 6.68E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene20225 | 0.819484 | 1.13E-52 | -1.69E-46 |
| MS.gene056796 | MS.gene20225 | 0.834342 | 3.17E-56 | -1.69E-46 |
| MS.gene056897 | MS.gene20225 | 0.829395 | 5.27E-55 | -1.69E-46 |
| MS.gene057011 | MS.gene20225 | 0.869688 | 2.57E-66 | -1.69E-46 |
| MS.gene05702 | MS.gene20225 | 0.817582 | 3.06E-52 | -1.69E-46 |
| MS.gene057048 | MS.gene20225 | 0.818008 | 2.45E-52 | -1.69E-46 |
| MS.gene057123 | MS.gene20225 | 0.817211 | 3.71E-52 | -1.69E-46 |
| MS.gene057124 | MS.gene20225 | 0.86684 | 2.12E-65 | -1.69E-46 |
| MS.gene057129 | MS.gene20225 | 0.832255 | 1.05E-55 | -1.69E-46 |
| MS.gene057146 | MS.gene20225 | 0.840328 | 9.29E-58 | -1.69E-46 |
| MS.gene057196 | MS.gene20225 | 0.831878 | 1.30E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene20225 | 0.806969 | 6.35E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene20225 | 0.810624 | 1.05E-50 | -1.69E-46 |
| MS.gene05733 | MS.gene20225 | 0.802578 | 5.25E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene20225 | 0.862455 | 5.01E-64 | -1.69E-46 |
| MS.gene057658 | MS.gene20225 | 0.815497 | 8.97E-52 | -1.69E-46 |
| MS.gene057749 | MS.gene20225 | 0.823696 | 1.21E-53 | -1.69E-46 |
| MS.gene057823 | MS.gene20225 | 0.836435 | 9.38E-57 | -1.69E-46 |
| MS.gene057845 | MS.gene20225 | 0.810983 | 8.79E-51 | -1.69E-46 |
| MS.gene057856 | MS.gene20225 | 0.820203 | 7.77E-53 | -1.69E-46 |
| MS.gene058006 | MS.gene20225 | 0.844277 | 8.35E-59 | -1.69E-46 |
| MS.gene058007 | MS.gene20225 | 0.805794 | 1.12E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene20225 | 0.8451 | 5.01E-59 | -1.69E-46 |
| MS.gene058233 | MS.gene20225 | 0.878259 | 3.24E-69 | -1.69E-46 |
| MS.gene058276 | MS.gene20225 | 0.806222 | 9.13E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene20225 | 0.868653 | 5.57E-66 | -1.69E-46 |
| MS.gene058323 | MS.gene20225 | 0.832791 | 7.73E-56 | -1.69E-46 |
| MS.gene058365 | MS.gene20225 | 0.808587 | 2.88E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene20225 | 0.84576 | 3.32E-59 | -1.69E-46 |
| MS.gene058419 | MS.gene20225 | 0.814196 | 1.74E-51 | -1.69E-46 |
| MS.gene058444 | MS.gene20225 | 0.859916 | 2.97E-63 | -1.69E-46 |
| MS.gene058544 | MS.gene20225 | 0.832136 | 1.12E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene20225 | 0.830925 | 2.23E-55 | -1.69E-46 |
| MS.gene058700 | MS.gene20225 | 0.846435 | 2.18E-59 | -1.69E-46 |
| MS.gene058787 | MS.gene20225 | 0.807057 | 6.09E-50 | -1.69E-46 |
| MS.gene058838 | MS.gene20225 | 0.818369 | 2.03E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene20225 | 0.802588 | 5.22E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene20225 | 0.815574 | 8.62E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene20225 | 0.811849 | 5.70E-51 | -1.69E-46 |
| MS.gene059141 | MS.gene20225 | 0.830965 | 2.18E-55 | -1.69E-46 |
| MS.gene05934 | MS.gene20225 | 0.82111 | 4.81E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene20225 | 0.819714 | 1.00E-52 | -1.69E-46 |
| MS.gene059455 | MS.gene20225 | 0.817029 | 4.07E-52 | -1.69E-46 |
| MS.gene059536 | MS.gene20225 | 0.850094 | 2.12E-60 | -1.69E-46 |
| MS.gene059597 | MS.gene20225 | 0.819055 | 1.42E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene20225 | 0.845139 | 4.89E-59 | -1.69E-46 |
| MS.gene059688 | MS.gene20225 | 0.883727 | 3.51E-71 | -1.69E-46 |
| MS.gene059692 | MS.gene20225 | 0.883156 | 5.70E-71 | -1.69E-46 |
| MS.gene059695 | MS.gene20225 | 0.887909 | 9.43E-73 | -1.69E-46 |
| MS.gene059718 | MS.gene20225 | 0.803695 | 3.08E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene20225 | 0.824632 | 7.27E-54 | -1.69E-46 |
| MS.gene060403 | MS.gene20225 | 0.877358 | 6.70E-69 | -1.69E-46 |
| MS.gene060407 | MS.gene20225 | 0.800475 | 1.42E-48 | -1.69E-46 |
| MS.gene060675 | MS.gene20225 | 0.837293 | 5.66E-57 | -1.69E-46 |
| MS.gene060804 | MS.gene20225 | 0.826627 | 2.44E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene20225 | 0.818954 | 1.50E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene20225 | 0.842144 | 3.09E-58 | -1.69E-46 |
| MS.gene06092 | MS.gene20225 | 0.899342 | 2.18E-77 | -1.69E-46 |
| MS.gene060986 | MS.gene20225 | 0.820312 | 7.33E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene20225 | 0.828707 | 7.74E-55 | -1.69E-46 |
| MS.gene061150 | MS.gene20225 | 0.824869 | 6.39E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene20225 | 0.819886 | 9.18E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene20225 | 0.829629 | 4.63E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20225.t1 | MTR_8g099615 | 95.714 | 140 | 6 | 0 | 1 | 140 | 1 | 140 | 1.08e-98 | 281 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20225.t1 | AT5G42070 | 78.571 | 70 | 15 | 0 | 65 | 134 | 69 | 138 | 8.61e-36 | 122 |
Find 47 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCTCTACGTTCAATTAA+AGG | 0.240046 | 4.4:-4675934 | MS.gene20225:CDS |
| TGTCCTGGATTTGTGGCTTC+TGG | 0.277176 | 4.4:-4676349 | MS.gene20225:CDS |
| GGTGGTATTGCAACAGTTCC+TGG | 0.294401 | 4.4:-4676406 | MS.gene20225:CDS |
| CATGGCCTGTATGCCTTTAT+AGG | 0.312442 | 4.4:+4676370 | None:intergenic |
| CTTTGGCTGGTGGCCTATAA+AGG | 0.340571 | 4.4:-4676383 | MS.gene20225:CDS |
| ATTGCAACAGTTCCTGGCTT+TGG | 0.355448 | 4.4:-4676400 | MS.gene20225:CDS |
| CAGGCCATGTCCTGGATTTG+TGG | 0.362074 | 4.4:-4676356 | MS.gene20225:CDS |
| CAACAGTTCCTGGCTTTGGC+TGG | 0.392114 | 4.4:-4676396 | MS.gene20225:CDS |
| CACCTCTACGTTCAATTAAA+GGG | 0.424582 | 4.4:-4675933 | MS.gene20225:CDS |
| CAGTTCCTGGCTTTGGCTGG+TGG | 0.448784 | 4.4:-4676393 | MS.gene20225:CDS |
| GCAGAAGAAATGAGTGAAGT+TGG | 0.456714 | 4.4:+4676624 | None:intergenic |
| CAATGGTGAGGGTAGGATGC+AGG | 0.456901 | 4.4:-4676428 | MS.gene20225:CDS |
| GAAACAGCAAGTTCATCATC+TGG | 0.480522 | 4.4:+4676600 | None:intergenic |
| GACGAGGTTGTCTCCGGTAG+TGG | 0.489301 | 4.4:-4676295 | MS.gene20225:CDS |
| CCACCAGAAGCCACAAATCC+AGG | 0.502695 | 4.4:+4676346 | None:intergenic |
| TGGATGGTACCTCTGTTTAG+TGG | 0.504482 | 4.4:+4676560 | None:intergenic |
| AAGGCATACAGGCCATGTCC+TGG | 0.519447 | 4.4:-4676364 | MS.gene20225:CDS |
| GGAACAAATATCTTAGTAGC+TGG | 0.533811 | 4.4:+4676663 | None:intergenic |
| TGGTGGTAGGTACAGACGAC+AGG | 0.534138 | 4.4:-4676329 | MS.gene20225:CDS |
| GATTTGTGGCTTCTGGTGGT+AGG | 0.534194 | 4.4:-4676342 | MS.gene20225:CDS |
| GACTTGGAGTCAGTTGCAGT+TGG | 0.535101 | 4.4:+4676259 | None:intergenic |
| ATAGGCCACCAGCCAAAGCC+AGG | 0.536722 | 4.4:+4676388 | None:intergenic |
| TCTAGTTAGTTCAAATGAGA+AGG | 0.537196 | 4.4:-4676120 | MS.gene20225:CDS |
| AGCATGGACGAGGTTGTCTC+CGG | 0.542240 | 4.4:-4676301 | MS.gene20225:CDS |
| GAAGTTGGAGAGCATAGTGT+GGG | 0.545271 | 4.4:+4676639 | None:intergenic |
| GGAAACGAAGAAATTGAGAA+AGG | 0.546629 | 4.4:-4676454 | MS.gene20225:CDS |
| TGAAGTTGGAGAGCATAGTG+TGG | 0.558341 | 4.4:+4676638 | None:intergenic |
| CCTGGATTTGTGGCTTCTGG+TGG | 0.563457 | 4.4:-4676346 | MS.gene20225:CDS |
| GAAATTGAGAAAGGATGCAA+TGG | 0.563789 | 4.4:-4676445 | MS.gene20225:CDS |
| TGATGATAATGTTGAGGTGT+TGG | 0.564881 | 4.4:+4676717 | None:intergenic |
| AAGGATGCAATGGTGAGGGT+AGG | 0.574706 | 4.4:-4676435 | MS.gene20225:CDS |
| AATGGTGAGGGTAGGATGCA+GGG | 0.575709 | 4.4:-4676427 | MS.gene20225:CDS |
| CAGACGACAGGGACAAAGCA+TGG | 0.576125 | 4.4:-4676317 | MS.gene20225:CDS |
| GGTGGCCTATAAAGGCATAC+AGG | 0.580795 | 4.4:-4676375 | MS.gene20225:CDS |
| GAGAAAGGATGCAATGGTGA+GGG | 0.581838 | 4.4:-4676439 | MS.gene20225:CDS |
| AGACAATAGATACCTTGACT+TGG | 0.607021 | 4.4:+4676243 | None:intergenic |
| TGAGAAAGGATGCAATGGTG+AGG | 0.617666 | 4.4:-4676440 | MS.gene20225:CDS |
| GTTGGAGAGCATAGTGTGGG+AGG | 0.638611 | 4.4:+4676642 | None:intergenic |
| AACAGCGGTTGCAAAGCATG+TGG | 0.642827 | 4.4:-4676475 | MS.gene20225:intron |
| ACAGGGACAAAGCATGGACG+AGG | 0.654227 | 4.4:-4676311 | MS.gene20225:CDS |
| AGCCCTTTAATTGAACGTAG+AGG | 0.660275 | 4.4:+4675931 | None:intergenic |
| CTGCAACTGACTCCAAGTCA+AGG | 0.664830 | 4.4:-4676255 | MS.gene20225:intron |
| TGAACGTGATGATAATGTTG+AGG | 0.683199 | 4.4:+4676711 | None:intergenic |
| GAAGCCACAAATCCAGGACA+TGG | 0.686791 | 4.4:+4676352 | None:intergenic |
| GGTGGTAGGTACAGACGACA+GGG | 0.692150 | 4.4:-4676328 | MS.gene20225:CDS |
| CAGAATTCTCCACTAAACAG+AGG | 0.708713 | 4.4:-4676569 | MS.gene20225:intron |
| GGTGAGGGTAGGATGCAGGG+TGG | 0.740434 | 4.4:-4676424 | MS.gene20225:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAAAAATAAAAAATA+GGG | + | chr4.4:4676630-4676649 | None:intergenic | 0.0% |
| !! | AAGATTTATCTATAAATAAA+TGG | + | chr4.4:4676481-4676500 | None:intergenic | 10.0% |
| !!! | AAAGATTTTTATTTAATGAA+GGG | + | chr4.4:4676139-4676158 | None:intergenic | 10.0% |
| !!! | TAAAGATTTTTATTTAATGA+AGG | + | chr4.4:4676140-4676159 | None:intergenic | 10.0% |
| !!! | AAGATTTTTATTTAATGAAG+GGG | + | chr4.4:4676138-4676157 | None:intergenic | 15.0% |
| !! | TCTATAAATAAATGGTAAAC+TGG | + | chr4.4:4676473-4676492 | None:intergenic | 20.0% |
| !!! | ATTTTGTTTATGTTGATAGA+AGG | - | chr4.4:4676591-4676610 | MS.gene20225:CDS | 20.0% |
| !!! | TTTTGTTTATGTTGATAGAA+GGG | - | chr4.4:4676592-4676611 | MS.gene20225:CDS | 20.0% |
| ! | TGTACAAACCAAAAAAATCA+TGG | - | chr4.4:4675982-4676001 | MS.gene20225:intron | 25.0% |
| ! | TTATTTAATGAAGGGGAAAA+TGG | + | chr4.4:4676131-4676150 | None:intergenic | 25.0% |
| !! | TTTGTTTATGTTGATAGAAG+GGG | - | chr4.4:4676593-4676612 | MS.gene20225:CDS | 25.0% |
| !!! | ATTTTTTATTTTGGTTCGCA+AGG | - | chr4.4:4676638-4676657 | MS.gene20225:CDS | 25.0% |
| !!! | CAAATGAGAAGGATTTTAAT+AGG | - | chr4.4:4676559-4676578 | MS.gene20225:intron | 25.0% |
| !!! | TTTTTTATTTTGGTTCGCAA+GGG | - | chr4.4:4676639-4676658 | MS.gene20225:CDS | 25.0% |
| CTTGATTCTAAGTCTTTCTT+AGG | - | chr4.4:4676711-4676730 | MS.gene20225:CDS | 30.0% | |
| TCTAGTTAGTTCAAATGAGA+AGG | - | chr4.4:4676548-4676567 | MS.gene20225:intron | 30.0% | |
| TGTCTCTTTCTCATGTATAT+GGG | - | chr4.4:4676443-4676462 | MS.gene20225:CDS | 30.0% | |
| TTGTCTCTTTCTCATGTATA+TGG | - | chr4.4:4676442-4676461 | MS.gene20225:CDS | 30.0% | |
| !!! | TGTTTTAATTTTGCCACTAC+CGG | + | chr4.4:4676389-4676408 | None:intergenic | 30.0% |
| !!! | TTTTTATTTTGGTTCGCAAG+GGG | - | chr4.4:4676640-4676659 | MS.gene20225:CDS | 30.0% |
| AGACAATAGATACCTTGACT+TGG | + | chr4.4:4676428-4676447 | None:intergenic | 35.0% | |
| CACCTCTACGTTCAATTAAA+GGG | - | chr4.4:4676735-4676754 | MS.gene20225:CDS | 35.0% | |
| GAAATTGAGAAAGGATGCAA+TGG | - | chr4.4:4676223-4676242 | MS.gene20225:intron | 35.0% | |
| GGAAACGAAGAAATTGAGAA+AGG | - | chr4.4:4676214-4676233 | MS.gene20225:intron | 35.0% | |
| GGAACAAATATCTTAGTAGC+TGG | + | chr4.4:4676008-4676027 | None:intergenic | 35.0% | |
| TCACCTCTACGTTCAATTAA+AGG | - | chr4.4:4676734-4676753 | MS.gene20225:CDS | 35.0% | |
| TTAATGAAGGGGAAAATGGA+TGG | + | chr4.4:4676127-4676146 | None:intergenic | 35.0% | |
| ! | AAGCTGCATCAAGACTTTTA+AGG | - | chr4.4:4676524-4676543 | MS.gene20225:intron | 35.0% |
| ! | AGCTGCATCAAGACTTTTAA+GGG | - | chr4.4:4676525-4676544 | MS.gene20225:intron | 35.0% |
| ! | TGAACGTGATGATAATGTTG+AGG | + | chr4.4:4675960-4675979 | None:intergenic | 35.0% |
| ! | TGATGATAATGTTGAGGTGT+TGG | + | chr4.4:4675954-4675973 | None:intergenic | 35.0% |
| AGCCCTTTAATTGAACGTAG+AGG | + | chr4.4:4676740-4676759 | None:intergenic | 40.0% | |
| CAGAATTCTCCACTAAACAG+AGG | - | chr4.4:4676099-4676118 | MS.gene20225:intron | 40.0% | |
| GAAACAGCAAGTTCATCATC+TGG | + | chr4.4:4676071-4676090 | None:intergenic | 40.0% | |
| GCAGAAGAAATGAGTGAAGT+TGG | + | chr4.4:4676047-4676066 | None:intergenic | 40.0% | |
| TTTGAGTAGTTGCTGAACAG+CGG | - | chr4.4:4676178-4676197 | MS.gene20225:intron | 40.0% | |
| !!! | GTAGCTGGCCATGATTTTTT+TGG | + | chr4.4:4675993-4676012 | None:intergenic | 40.0% |
| ATTGCAACAGTTCCTGGCTT+TGG | - | chr4.4:4676268-4676287 | MS.gene20225:CDS | 45.0% | |
| CATGGCCTGTATGCCTTTAT+AGG | + | chr4.4:4676301-4676320 | None:intergenic | 45.0% | |
| GAGAAAGGATGCAATGGTGA+GGG | - | chr4.4:4676229-4676248 | MS.gene20225:intron | 45.0% | |
| TGAGAAAGGATGCAATGGTG+AGG | - | chr4.4:4676228-4676247 | MS.gene20225:intron | 45.0% | |
| TGGATGGTACCTCTGTTTAG+TGG | + | chr4.4:4676111-4676130 | None:intergenic | 45.0% | |
| ! | GAAGTTGGAGAGCATAGTGT+GGG | + | chr4.4:4676032-4676051 | None:intergenic | 45.0% |
| ! | TGAAGTTGGAGAGCATAGTG+TGG | + | chr4.4:4676033-4676052 | None:intergenic | 45.0% |
| !! | CAAAATAAAAAATAAAAAAT+AGG | + | chr4.4:4676631-4676650 | None:intergenic | 5.0% |
| !!! | CTATTTTTTATTTTTTATTT+TGG | - | chr4.4:4676629-4676648 | MS.gene20225:CDS | 5.0% |
| AACAGCGGTTGCAAAGCATG+TGG | - | chr4.4:4676193-4676212 | MS.gene20225:intron | 50.0% | |
| AAGGATGCAATGGTGAGGGT+AGG | - | chr4.4:4676233-4676252 | MS.gene20225:intron | 50.0% | |
| AATGGTGAGGGTAGGATGCA+GGG | - | chr4.4:4676241-4676260 | MS.gene20225:intron | 50.0% | |
| CTTTGGCTGGTGGCCTATAA+AGG | - | chr4.4:4676285-4676304 | MS.gene20225:CDS | 50.0% | |
| GAAGCCACAAATCCAGGACA+TGG | + | chr4.4:4676319-4676338 | None:intergenic | 50.0% | |
| GACTTGGAGTCAGTTGCAGT+TGG | + | chr4.4:4676412-4676431 | None:intergenic | 50.0% | |
| GATTTGTGGCTTCTGGTGGT+AGG | - | chr4.4:4676326-4676345 | MS.gene20225:CDS | 50.0% | |
| GGTGGTATTGCAACAGTTCC+TGG | - | chr4.4:4676262-4676281 | MS.gene20225:CDS | 50.0% | |
| TGTCCTGGATTTGTGGCTTC+TGG | - | chr4.4:4676319-4676338 | MS.gene20225:CDS | 50.0% | |
| ! | CTGCAACTGACTCCAAGTCA+AGG | - | chr4.4:4676413-4676432 | MS.gene20225:CDS | 50.0% |
| ! | GGTGGCCTATAAAGGCATAC+AGG | - | chr4.4:4676293-4676312 | MS.gene20225:CDS | 50.0% |
| AAGGCATACAGGCCATGTCC+TGG | - | chr4.4:4676304-4676323 | MS.gene20225:CDS | 55.0% | |
| ACAGGGACAAAGCATGGACG+AGG | - | chr4.4:4676357-4676376 | MS.gene20225:CDS | 55.0% | |
| AGCATGGACGAGGTTGTCTC+CGG | - | chr4.4:4676367-4676386 | MS.gene20225:CDS | 55.0% | |
| CAATGGTGAGGGTAGGATGC+AGG | - | chr4.4:4676240-4676259 | MS.gene20225:intron | 55.0% | |
| CAGACGACAGGGACAAAGCA+TGG | - | chr4.4:4676351-4676370 | MS.gene20225:CDS | 55.0% | |
| CAGGCCATGTCCTGGATTTG+TGG | - | chr4.4:4676312-4676331 | MS.gene20225:CDS | 55.0% | |
| CCACCAGAAGCCACAAATCC+AGG | + | chr4.4:4676325-4676344 | None:intergenic | 55.0% | |
| CCTGGATTTGTGGCTTCTGG+TGG | - | chr4.4:4676322-4676341 | MS.gene20225:CDS | 55.0% | |
| GGTGGTAGGTACAGACGACA+GGG | - | chr4.4:4676340-4676359 | MS.gene20225:CDS | 55.0% | |
| TGGTGGTAGGTACAGACGAC+AGG | - | chr4.4:4676339-4676358 | MS.gene20225:CDS | 55.0% | |
| ! | GTTGGAGAGCATAGTGTGGG+AGG | + | chr4.4:4676029-4676048 | None:intergenic | 55.0% |
| !! | CAACAGTTCCTGGCTTTGGC+TGG | - | chr4.4:4676272-4676291 | MS.gene20225:CDS | 55.0% |
| ATAGGCCACCAGCCAAAGCC+AGG | + | chr4.4:4676283-4676302 | None:intergenic | 60.0% | |
| GACGAGGTTGTCTCCGGTAG+TGG | - | chr4.4:4676373-4676392 | MS.gene20225:CDS | 60.0% | |
| !! | CAGTTCCTGGCTTTGGCTGG+TGG | - | chr4.4:4676275-4676294 | MS.gene20225:CDS | 60.0% |
| GGTGAGGGTAGGATGCAGGG+TGG | - | chr4.4:4676244-4676263 | MS.gene20225:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 4675927 | 4676763 | 4675927 | ID=MS.gene20225 |
| chr4.4 | mRNA | 4675927 | 4676763 | 4675927 | ID=MS.gene20225.t1;Parent=MS.gene20225 |
| chr4.4 | exon | 4676570 | 4676763 | 4676570 | ID=MS.gene20225.t1.exon1;Parent=MS.gene20225.t1 |
| chr4.4 | CDS | 4676570 | 4676763 | 4676570 | ID=cds.MS.gene20225.t1;Parent=MS.gene20225.t1 |
| chr4.4 | exon | 4676256 | 4676492 | 4676256 | ID=MS.gene20225.t1.exon2;Parent=MS.gene20225.t1 |
| chr4.4 | CDS | 4676256 | 4676492 | 4676256 | ID=cds.MS.gene20225.t1;Parent=MS.gene20225.t1 |
| chr4.4 | exon | 4676110 | 4676163 | 4676110 | ID=MS.gene20225.t1.exon3;Parent=MS.gene20225.t1 |
| chr4.4 | CDS | 4676110 | 4676163 | 4676110 | ID=cds.MS.gene20225.t1;Parent=MS.gene20225.t1 |
| chr4.4 | exon | 4675927 | 4675957 | 4675927 | ID=MS.gene20225.t1.exon4;Parent=MS.gene20225.t1 |
| chr4.4 | CDS | 4675927 | 4675957 | 4675927 | ID=cds.MS.gene20225.t1;Parent=MS.gene20225.t1 |
| Gene Sequence |
| Protein sequence |
