Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28158.t1 | XP_003630120.1 | 94.9 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 2.90E-87 | 331.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28158.t1 | Q9XIK3 | 65.4 | 185 | 46 | 4 | 7 | 179 | 2 | 180 | 7.7e-57 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28158.t1 | G7LBB6 | 94.9 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 2.1e-87 | 331.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene28158 | 0.838024 | 3.68E-57 | -1.69E-46 |
| MS.gene049222 | MS.gene28158 | 0.851528 | 8.37E-61 | -1.69E-46 |
| MS.gene049285 | MS.gene28158 | 0.82279 | 1.96E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene28158 | -0.823108 | 1.65E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene28158 | 0.81955 | 1.09E-52 | -1.69E-46 |
| MS.gene04932 | MS.gene28158 | -0.807118 | 5.91E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene28158 | 0.833081 | 6.54E-56 | -1.69E-46 |
| MS.gene049633 | MS.gene28158 | 0.873602 | 1.29E-67 | -1.69E-46 |
| MS.gene050170 | MS.gene28158 | 0.881745 | 1.86E-70 | -1.69E-46 |
| MS.gene050323 | MS.gene28158 | 0.861147 | 1.26E-63 | -1.69E-46 |
| MS.gene050338 | MS.gene28158 | -0.85111 | 1.10E-60 | -1.69E-46 |
| MS.gene050444 | MS.gene28158 | 0.856984 | 2.22E-62 | -1.69E-46 |
| MS.gene050496 | MS.gene28158 | 0.840858 | 6.75E-58 | -1.69E-46 |
| MS.gene050504 | MS.gene28158 | 0.816681 | 4.88E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene28158 | 0.85051 | 1.62E-60 | -1.69E-46 |
| MS.gene050612 | MS.gene28158 | 0.820192 | 7.81E-53 | -1.69E-46 |
| MS.gene050675 | MS.gene28158 | 0.822415 | 2.40E-53 | -1.69E-46 |
| MS.gene050697 | MS.gene28158 | 0.81735 | 3.45E-52 | -1.69E-46 |
| MS.gene050699 | MS.gene28158 | 0.817811 | 2.72E-52 | -1.69E-46 |
| MS.gene050747 | MS.gene28158 | 0.83829 | 3.14E-57 | -1.69E-46 |
| MS.gene050772 | MS.gene28158 | 0.826046 | 3.36E-54 | -1.69E-46 |
| MS.gene05080 | MS.gene28158 | 0.866252 | 3.27E-65 | -1.69E-46 |
| MS.gene05081 | MS.gene28158 | 0.83338 | 5.51E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene28158 | 0.879154 | 1.57E-69 | -1.69E-46 |
| MS.gene050871 | MS.gene28158 | 0.876187 | 1.70E-68 | -1.69E-46 |
| MS.gene050904 | MS.gene28158 | 0.861154 | 1.25E-63 | -1.69E-46 |
| MS.gene050950 | MS.gene28158 | 0.841533 | 4.49E-58 | -1.69E-46 |
| MS.gene051268 | MS.gene28158 | 0.831564 | 1.55E-55 | -1.69E-46 |
| MS.gene051317 | MS.gene28158 | 0.849038 | 4.18E-60 | -1.69E-46 |
| MS.gene051438 | MS.gene28158 | 0.839135 | 1.90E-57 | -1.69E-46 |
| MS.gene051439 | MS.gene28158 | -0.820058 | 8.38E-53 | -1.69E-46 |
| MS.gene051617 | MS.gene28158 | 0.828278 | 9.83E-55 | -1.69E-46 |
| MS.gene051651 | MS.gene28158 | -0.815635 | 8.35E-52 | -1.69E-46 |
| MS.gene051666 | MS.gene28158 | 0.802168 | 6.38E-49 | -1.69E-46 |
| MS.gene051734 | MS.gene28158 | 0.809478 | 1.86E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene28158 | 0.906805 | 1.00E-80 | -1.69E-46 |
| MS.gene051874 | MS.gene28158 | 0.818591 | 1.81E-52 | -1.69E-46 |
| MS.gene051914 | MS.gene28158 | -0.81393 | 1.99E-51 | -1.69E-46 |
| MS.gene051941 | MS.gene28158 | 0.822964 | 1.79E-53 | -1.69E-46 |
| MS.gene052129 | MS.gene28158 | -0.827303 | 1.69E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene28158 | 0.848747 | 5.03E-60 | -1.69E-46 |
| MS.gene052308 | MS.gene28158 | -0.815999 | 6.93E-52 | -1.69E-46 |
| MS.gene052469 | MS.gene28158 | 0.810743 | 9.91E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene28158 | 0.803976 | 2.70E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene28158 | 0.808122 | 3.62E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene28158 | 0.845396 | 4.17E-59 | -1.69E-46 |
| MS.gene052506 | MS.gene28158 | 0.816984 | 4.17E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene28158 | 0.808747 | 2.66E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene28158 | 0.822436 | 2.37E-53 | -1.69E-46 |
| MS.gene052823 | MS.gene28158 | 0.839644 | 1.40E-57 | -1.69E-46 |
| MS.gene052825 | MS.gene28158 | 0.842828 | 2.04E-58 | -1.69E-46 |
| MS.gene052870 | MS.gene28158 | 0.864801 | 9.36E-65 | -1.69E-46 |
| MS.gene052872 | MS.gene28158 | 0.8791 | 1.64E-69 | -1.69E-46 |
| MS.gene052966 | MS.gene28158 | 0.826942 | 2.06E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene28158 | 0.850048 | 2.18E-60 | -1.69E-46 |
| MS.gene053207 | MS.gene28158 | 0.801691 | 7.99E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene28158 | 0.874806 | 5.06E-68 | -1.69E-46 |
| MS.gene053480 | MS.gene28158 | 0.865151 | 7.27E-65 | -1.69E-46 |
| MS.gene053574 | MS.gene28158 | 0.828117 | 1.07E-54 | -1.69E-46 |
| MS.gene053632 | MS.gene28158 | 0.820917 | 5.32E-53 | -1.69E-46 |
| MS.gene053824 | MS.gene28158 | 0.88981 | 1.74E-73 | -1.69E-46 |
| MS.gene053912 | MS.gene28158 | 0.819438 | 1.16E-52 | -1.69E-46 |
| MS.gene053916 | MS.gene28158 | 0.84674 | 1.80E-59 | -1.69E-46 |
| MS.gene054107 | MS.gene28158 | 0.811082 | 8.36E-51 | -1.69E-46 |
| MS.gene054344 | MS.gene28158 | 0.8295 | 4.97E-55 | -1.69E-46 |
| MS.gene054408 | MS.gene28158 | 0.808285 | 3.34E-50 | -1.69E-46 |
| MS.gene054593 | MS.gene28158 | -0.806987 | 6.30E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene28158 | 0.825715 | 4.03E-54 | -1.69E-46 |
| MS.gene054633 | MS.gene28158 | -0.833601 | 4.86E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene28158 | 0.800767 | 1.23E-48 | -1.69E-46 |
| MS.gene054706 | MS.gene28158 | -0.825097 | 5.64E-54 | -1.69E-46 |
| MS.gene05470 | MS.gene28158 | 0.864077 | 1.58E-64 | -1.69E-46 |
| MS.gene054850 | MS.gene28158 | -0.823915 | 1.07E-53 | -1.69E-46 |
| MS.gene054884 | MS.gene28158 | -0.838254 | 3.21E-57 | -1.69E-46 |
| MS.gene054941 | MS.gene28158 | -0.837691 | 4.48E-57 | -1.69E-46 |
| MS.gene05494 | MS.gene28158 | 0.882371 | 1.10E-70 | -1.69E-46 |
| MS.gene054954 | MS.gene28158 | 0.814461 | 1.52E-51 | -1.69E-46 |
| MS.gene05505 | MS.gene28158 | 0.805648 | 1.21E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene28158 | 0.809741 | 1.63E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene28158 | 0.844672 | 6.54E-59 | -1.69E-46 |
| MS.gene055289 | MS.gene28158 | 0.80067 | 1.29E-48 | -1.69E-46 |
| MS.gene055362 | MS.gene28158 | 0.811837 | 5.73E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene28158 | 0.822229 | 2.65E-53 | -1.69E-46 |
| MS.gene055539 | MS.gene28158 | 0.804981 | 1.66E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene28158 | 0.865515 | 5.59E-65 | -1.69E-46 |
| MS.gene055741 | MS.gene28158 | 0.828983 | 6.64E-55 | -1.69E-46 |
| MS.gene055744 | MS.gene28158 | 0.81908 | 1.40E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene28158 | 0.870846 | 1.07E-66 | -1.69E-46 |
| MS.gene055750 | MS.gene28158 | 0.866414 | 2.90E-65 | -1.69E-46 |
| MS.gene055751 | MS.gene28158 | 0.86993 | 2.14E-66 | -1.69E-46 |
| MS.gene05589 | MS.gene28158 | 0.882437 | 1.04E-70 | -1.69E-46 |
| MS.gene055941 | MS.gene28158 | 0.836996 | 6.74E-57 | -1.69E-46 |
| MS.gene056266 | MS.gene28158 | 0.817838 | 2.68E-52 | -1.69E-46 |
| MS.gene056283 | MS.gene28158 | 0.865982 | 3.98E-65 | -1.69E-46 |
| MS.gene056387 | MS.gene28158 | 0.912458 | 1.90E-83 | -1.69E-46 |
| MS.gene056446 | MS.gene28158 | 0.805614 | 1.23E-49 | -1.69E-46 |
| MS.gene056487 | MS.gene28158 | 0.803047 | 4.20E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene28158 | 0.85429 | 1.36E-61 | -1.69E-46 |
| MS.gene056510 | MS.gene28158 | 0.823966 | 1.04E-53 | -1.69E-46 |
| MS.gene05652 | MS.gene28158 | -0.81077 | 9.77E-51 | -1.69E-46 |
| MS.gene056618 | MS.gene28158 | -0.845032 | 5.23E-59 | -1.69E-46 |
| MS.gene05664 | MS.gene28158 | 0.801411 | 9.12E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene28158 | 0.819495 | 1.13E-52 | -1.69E-46 |
| MS.gene05666 | MS.gene28158 | 0.834808 | 2.42E-56 | -1.69E-46 |
| MS.gene056688 | MS.gene28158 | -0.816593 | 5.10E-52 | -1.69E-46 |
| MS.gene056692 | MS.gene28158 | 0.832468 | 9.29E-56 | -1.69E-46 |
| MS.gene056694 | MS.gene28158 | -0.81297 | 3.24E-51 | -1.69E-46 |
| MS.gene056697 | MS.gene28158 | -0.838297 | 3.13E-57 | -1.69E-46 |
| MS.gene057076 | MS.gene28158 | 0.853414 | 2.43E-61 | -1.69E-46 |
| MS.gene057086 | MS.gene28158 | 0.810595 | 1.07E-50 | -1.69E-46 |
| MS.gene057332 | MS.gene28158 | 0.811997 | 5.29E-51 | -1.69E-46 |
| MS.gene057367 | MS.gene28158 | -0.822749 | 2.01E-53 | -1.69E-46 |
| MS.gene057658 | MS.gene28158 | 0.856896 | 2.36E-62 | -1.69E-46 |
| MS.gene057717 | MS.gene28158 | -0.823967 | 1.04E-53 | -1.69E-46 |
| MS.gene057734 | MS.gene28158 | 0.838474 | 2.81E-57 | -1.69E-46 |
| MS.gene057831 | MS.gene28158 | 0.830618 | 2.65E-55 | -1.69E-46 |
| MS.gene057856 | MS.gene28158 | 0.80996 | 1.46E-50 | -1.69E-46 |
| MS.gene057857 | MS.gene28158 | 0.842475 | 2.53E-58 | -1.69E-46 |
| MS.gene05787 | MS.gene28158 | 0.829528 | 4.90E-55 | -1.69E-46 |
| MS.gene058090 | MS.gene28158 | 0.818769 | 1.65E-52 | -1.69E-46 |
| MS.gene058120 | MS.gene28158 | 0.80118 | 1.02E-48 | -1.69E-46 |
| MS.gene058128 | MS.gene28158 | -0.815982 | 6.99E-52 | -1.69E-46 |
| MS.gene058130 | MS.gene28158 | 0.80381 | 2.92E-49 | -1.69E-46 |
| MS.gene058157 | MS.gene28158 | 0.807284 | 5.45E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene28158 | -0.817302 | 3.54E-52 | -1.69E-46 |
| MS.gene058338 | MS.gene28158 | 0.820439 | 6.86E-53 | -1.69E-46 |
| MS.gene05837 | MS.gene28158 | 0.837282 | 5.70E-57 | -1.69E-46 |
| MS.gene058382 | MS.gene28158 | -0.81404 | 1.89E-51 | -1.69E-46 |
| MS.gene058418 | MS.gene28158 | -0.809806 | 1.58E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene28158 | 0.823675 | 1.22E-53 | -1.69E-46 |
| MS.gene058448 | MS.gene28158 | 0.836451 | 9.29E-57 | -1.69E-46 |
| MS.gene058544 | MS.gene28158 | 0.826264 | 2.98E-54 | -1.69E-46 |
| MS.gene058723 | MS.gene28158 | 0.825252 | 5.19E-54 | -1.69E-46 |
| MS.gene058773 | MS.gene28158 | 0.816636 | 4.99E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene28158 | 0.870566 | 1.33E-66 | -1.69E-46 |
| MS.gene058853 | MS.gene28158 | -0.833652 | 4.72E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene28158 | 0.804162 | 2.47E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene28158 | 0.876318 | 1.54E-68 | -1.69E-46 |
| MS.gene05914 | MS.gene28158 | 0.822547 | 2.23E-53 | -1.69E-46 |
| MS.gene05926 | MS.gene28158 | -0.872198 | 3.82E-67 | -1.69E-46 |
| MS.gene05934 | MS.gene28158 | 0.821247 | 4.47E-53 | -1.69E-46 |
| MS.gene05960 | MS.gene28158 | -0.814102 | 1.83E-51 | -1.69E-46 |
| MS.gene059616 | MS.gene28158 | -0.814258 | 1.69E-51 | -1.69E-46 |
| MS.gene059667 | MS.gene28158 | 0.85105 | 1.14E-60 | -1.69E-46 |
| MS.gene059677 | MS.gene28158 | 0.828719 | 7.69E-55 | -1.69E-46 |
| MS.gene059699 | MS.gene28158 | -0.816332 | 5.84E-52 | -1.69E-46 |
| MS.gene059703 | MS.gene28158 | -0.812474 | 4.16E-51 | -1.69E-46 |
| MS.gene059717 | MS.gene28158 | 0.821062 | 4.93E-53 | -1.69E-46 |
| MS.gene059718 | MS.gene28158 | 0.830775 | 2.43E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene28158 | 0.841729 | 3.98E-58 | -1.69E-46 |
| MS.gene06000 | MS.gene28158 | 0.874965 | 4.47E-68 | -1.69E-46 |
| MS.gene06027 | MS.gene28158 | 0.839744 | 1.32E-57 | -1.69E-46 |
| MS.gene060405 | MS.gene28158 | -0.829297 | 5.57E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene28158 | 0.865096 | 7.57E-65 | -1.69E-46 |
| MS.gene060778 | MS.gene28158 | 0.829307 | 5.54E-55 | -1.69E-46 |
| MS.gene06077 | MS.gene28158 | 0.817876 | 2.63E-52 | -1.69E-46 |
| MS.gene060835 | MS.gene28158 | 0.82496 | 6.08E-54 | -1.69E-46 |
| MS.gene06092 | MS.gene28158 | 0.817802 | 2.73E-52 | -1.69E-46 |
| MS.gene06097 | MS.gene28158 | 0.829037 | 6.44E-55 | -1.69E-46 |
| MS.gene060982 | MS.gene28158 | 0.867341 | 1.47E-65 | -1.69E-46 |
| MS.gene061044 | MS.gene28158 | -0.813543 | 2.43E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene28158 | 0.812327 | 4.48E-51 | -1.69E-46 |
| MS.gene061231 | MS.gene28158 | 0.802028 | 6.81E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28158.t1 | MTR_8g092030 | 94.944 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 3.65e-111 | 314 |
| MS.gene28158.t1 | MTR_8g092030 | 94.706 | 170 | 8 | 1 | 3 | 171 | 1 | 170 | 6.05e-105 | 298 |
| MS.gene28158.t1 | MTR_1g060070 | 36.134 | 119 | 73 | 2 | 57 | 175 | 8 | 123 | 1.53e-21 | 85.5 |
| MS.gene28158.t1 | MTR_7g104600 | 34.711 | 121 | 73 | 4 | 58 | 175 | 39 | 156 | 1.43e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28158.t1 | AT1G10500 | 64.835 | 182 | 52 | 3 | 7 | 179 | 2 | 180 | 2.21e-76 | 226 |
| MS.gene28158.t1 | AT2G16710 | 37.719 | 114 | 63 | 3 | 67 | 175 | 14 | 124 | 1.28e-21 | 85.9 |
| MS.gene28158.t1 | AT2G16710 | 35.593 | 118 | 66 | 3 | 67 | 175 | 14 | 130 | 3.82e-21 | 84.7 |
| MS.gene28158.t1 | AT2G36260 | 33.962 | 106 | 66 | 3 | 70 | 175 | 5 | 106 | 1.17e-17 | 74.7 |
| MS.gene28158.t1 | AT5G03905 | 34.234 | 111 | 70 | 2 | 70 | 179 | 50 | 158 | 3.37e-17 | 75.1 |
| MS.gene28158.t1 | AT2G16710 | 35.366 | 82 | 50 | 2 | 70 | 151 | 22 | 100 | 7.44e-13 | 62.4 |
Find 42 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGCCTACTCTTCGTATT+TGG | 0.197827 | 4.4:+9690076 | MS.gene28158:CDS |
| GATCGAATTGAGAGAGGTTT+AGG | 0.272109 | 4.4:-9688543 | None:intergenic |
| CCACAGGTTTGTGTCGCATT+AGG | 0.312594 | 4.4:-9690154 | None:intergenic |
| CAAGATTTGTGTTTAAGAAT+TGG | 0.321991 | 4.4:+9689228 | MS.gene28158:CDS |
| GATTGGAACGCGAACGAATT+GGG | 0.333118 | 4.4:-9688463 | None:intergenic |
| AATACGAAGAGTAGGCTCTT+AGG | 0.361492 | 4.4:-9690073 | None:intergenic |
| TGATTGGAACGCGAACGAAT+TGG | 0.382823 | 4.4:-9688464 | None:intergenic |
| GGTGCCACGGCCCCAGATGC+AGG | 0.406436 | 4.4:-9689144 | None:intergenic |
| ATCGAATTGAGAGAGGTTTA+GGG | 0.409502 | 4.4:-9688542 | None:intergenic |
| GAGAGAGAGCTATGAGTGAT+TGG | 0.409580 | 4.4:-9688480 | None:intergenic |
| TGCACCTGCATCTGGGGCCG+TGG | 0.436538 | 4.4:+9689140 | MS.gene28158:CDS |
| AAAACCTTTGTATTCAATGA+TGG | 0.441255 | 4.4:-9689328 | None:intergenic |
| CTAAAGCGGCGATTGAGAAT+TGG | 0.446890 | 4.4:-9688519 | None:intergenic |
| GACTACAGTGATGCTCTGAT+TGG | 0.458599 | 4.4:+9690109 | MS.gene28158:CDS |
| GTCAAACAAGGTGGGTGCTC+TGG | 0.472114 | 4.4:+9689252 | MS.gene28158:CDS |
| TTATCTGTAAGCGAAATTGC+AGG | 0.509358 | 4.4:-9689165 | None:intergenic |
| GGGCTTACCGGCAGCAATAA+CGG | 0.512066 | 4.4:-9688571 | None:intergenic |
| ATTGGAACGCGAACGAATTG+GGG | 0.513543 | 4.4:-9688462 | None:intergenic |
| AAATGATTTACCACATCCAC+AGG | 0.517325 | 4.4:-9690170 | None:intergenic |
| AAATCAAACATGGCCATGGC+TGG | 0.519320 | 4.4:+9688399 | None:intergenic |
| TATTCAATGATGGAGTCGTC+TGG | 0.526703 | 4.4:-9689318 | None:intergenic |
| TACAGTGATGCTCTGATTGG+AGG | 0.527694 | 4.4:+9690112 | MS.gene28158:CDS |
| TCACTTTCCGTTATTGCTGC+CGG | 0.540114 | 4.4:+9688564 | MS.gene28158:CDS |
| TTTAGCTGCACCTGCATCTG+GGG | 0.577576 | 4.4:+9689134 | MS.gene28158:intron |
| GCGACACAAACCTGTGGATG+TGG | 0.581388 | 4.4:+9690160 | MS.gene28158:CDS |
| TTAAGAATTGGTGTCAAACA+AGG | 0.584994 | 4.4:+9689240 | MS.gene28158:CDS |
| AAGCGAAATTGCAGGTGCCA+CGG | 0.587731 | 4.4:-9689157 | None:intergenic |
| AGAATTGGTGTCAAACAAGG+TGG | 0.587930 | 4.4:+9689243 | MS.gene28158:CDS |
| AGTGATGCTCTGATTGGAGG+AGG | 0.589985 | 4.4:+9690115 | MS.gene28158:CDS |
| TTGAGGACAGGGCTAATGCA+AGG | 0.598318 | 4.4:+9689295 | MS.gene28158:CDS |
| CAATTACTACTACCGCGCAC+TGG | 0.599076 | 4.4:+9688436 | MS.gene28158:CDS |
| AGAGAGGTTTAGGGCTAAAG+CGG | 0.601518 | 4.4:-9688533 | None:intergenic |
| GCATTCCAAATACGAAGAGT+AGG | 0.603532 | 4.4:-9690081 | None:intergenic |
| ACACAAATCAAACATGGCCA+TGG | 0.605803 | 4.4:+9688395 | None:intergenic |
| AGAGAAAGCAGCACCAGCCA+TGG | 0.608264 | 4.4:-9688412 | None:intergenic |
| CTCTGGTATGTCATACACAA+TGG | 0.609613 | 4.4:+9689269 | MS.gene28158:CDS |
| GAAAGTGATCGAATTGAGAG+AGG | 0.627615 | 4.4:-9688549 | None:intergenic |
| TCAATCACTTGAATAAGATG+AGG | 0.638619 | 4.4:+9689193 | MS.gene28158:CDS |
| GAATTGGTGTCAAACAAGGT+GGG | 0.648767 | 4.4:+9689244 | MS.gene28158:CDS |
| GACTCCATCATTGAATACAA+AGG | 0.682577 | 4.4:+9689324 | MS.gene28158:CDS |
| CCTAATGCGACACAAACCTG+TGG | 0.690717 | 4.4:+9690154 | MS.gene28158:CDS |
| GAATTGGGGAGACCAGTGCG+CGG | 0.788727 | 4.4:-9688448 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAGAGAAAAAAAAAA+TGG | + | chr4.4:9688989-9689008 | MS.gene28158:intron | 10.0% |
| !! | AATAAAGAGAAAAAAAAAAT+GGG | + | chr4.4:9688990-9689009 | MS.gene28158:intron | 10.0% |
| !! | ATTAATAAATAGAAGAATAT+GGG | - | chr4.4:9688595-9688614 | None:intergenic | 10.0% |
| !! | GATTAATAAATAGAAGAATA+TGG | - | chr4.4:9688596-9688615 | None:intergenic | 15.0% |
| !! | TTAATAAATAGAAGAATATG+GGG | - | chr4.4:9688594-9688613 | None:intergenic | 15.0% |
| !!! | ATATTTTGCTTGTTTTTATT+TGG | + | chr4.4:9689561-9689580 | MS.gene28158:intron | 15.0% |
| !!! | TATTTTGCTTGTTTTTATTT+GGG | + | chr4.4:9689562-9689581 | MS.gene28158:intron | 15.0% |
| !! | AGGAAATAACAAAACATAAA+TGG | - | chr4.4:9688807-9688826 | None:intergenic | 20.0% |
| !! | ATCAAATAATTTAATCACCT+TGG | - | chr4.4:9689414-9689433 | None:intergenic | 20.0% |
| !! | CAAAATACTCCTATTAATTA+GGG | - | chr4.4:9688771-9688790 | None:intergenic | 20.0% |
| !! | CAATATCTTCAAATGAATAT+GGG | + | chr4.4:9689450-9689469 | MS.gene28158:intron | 20.0% |
| !! | TCAAAATACTCCTATTAATT+AGG | - | chr4.4:9688772-9688791 | None:intergenic | 20.0% |
| !!! | AGTTTGATAACATTTATACT+TGG | - | chr4.4:9689602-9689621 | None:intergenic | 20.0% |
| !!! | TTTATTTTAAACTCATAGCA+TGG | - | chr4.4:9688976-9688995 | None:intergenic | 20.0% |
| ! | AAAACCTTTGTATTCAATGA+TGG | - | chr4.4:9689331-9689350 | None:intergenic | 25.0% |
| ! | ATTACGACAATTTCATGAAT+TGG | - | chr4.4:9688655-9688674 | None:intergenic | 25.0% |
| ! | CAAGATTTGTGTTTAAGAAT+TGG | + | chr4.4:9689228-9689247 | MS.gene28158:CDS | 25.0% |
| ! | CAATGATAATAAAAACTGAG+TGG | + | chr4.4:9689629-9689648 | MS.gene28158:intron | 25.0% |
| ! | CCAATATCTTCAAATGAATA+TGG | + | chr4.4:9689449-9689468 | MS.gene28158:intron | 25.0% |
| ! | CCATATTCATTTGAAGATAT+TGG | - | chr4.4:9689452-9689471 | None:intergenic | 25.0% |
| ! | GCAAATGTTGAATAAATACA+TGG | - | chr4.4:9689101-9689120 | None:intergenic | 25.0% |
| !! | ATTAAGATTTTCATCGATAC+TGG | - | chr4.4:9689478-9689497 | None:intergenic | 25.0% |
| !! | CTTTTAGTAGTAATTAAGCT+TGG | + | chr4.4:9689975-9689994 | MS.gene28158:intron | 25.0% |
| !!! | AGGAGTATTTTGAAAGATTA+AGG | + | chr4.4:9688779-9688798 | MS.gene28158:intron | 25.0% |
| !!! | ATTAAACTTTTTTCGCTGAT+AGG | + | chr4.4:9689663-9689682 | MS.gene28158:intron | 25.0% |
| !!! | CTTTTTAAGCTTTTGTTTAC+TGG | + | chr4.4:9689889-9689908 | MS.gene28158:intron | 25.0% |
| !!! | GCAGTTCAAACTTTTTTTTA+TGG | + | chr4.4:9689938-9689957 | MS.gene28158:intron | 25.0% |
| ACAGATATGATAGTTAACAG+AGG | - | chr4.4:9689717-9689736 | None:intergenic | 30.0% | |
| ATACTCCTATTAATTAGGGT+AGG | - | chr4.4:9688767-9688786 | None:intergenic | 30.0% | |
| TCAATCACTTGAATAAGATG+AGG | + | chr4.4:9689193-9689212 | MS.gene28158:CDS | 30.0% | |
| TTAAGACTTGATTGAAAGCT+TGG | + | chr4.4:9689840-9689859 | MS.gene28158:intron | 30.0% | |
| !! | GAATACAAAGGTTTTGTCAT+TGG | + | chr4.4:9689336-9689355 | MS.gene28158:CDS | 30.0% |
| !! | TTAAGAATTGGTGTCAAACA+AGG | + | chr4.4:9689240-9689259 | MS.gene28158:CDS | 30.0% |
| !! | TTGTTATTTCCTATGAACCA+CGG | + | chr4.4:9688815-9688834 | MS.gene28158:intron | 30.0% |
| !!! | TTTTTTTTATGGCTGCACTT+GGG | + | chr4.4:9689949-9689968 | MS.gene28158:intron | 30.0% |
| AAATGATTTACCACATCCAC+AGG | - | chr4.4:9690173-9690192 | None:intergenic | 35.0% | |
| AAGAACTGAAGAAGATAACG+TGG | - | chr4.4:9689872-9689891 | None:intergenic | 35.0% | |
| ATCGAATTGAGAGAGGTTTA+GGG | - | chr4.4:9688545-9688564 | None:intergenic | 35.0% | |
| ATTAATTAGGGTAGGCAAAG+CGG | - | chr4.4:9688759-9688778 | None:intergenic | 35.0% | |
| CTTTGCCTACCCTAATTAAT+AGG | + | chr4.4:9688759-9688778 | MS.gene28158:intron | 35.0% | |
| GACTCCATCATTGAATACAA+AGG | + | chr4.4:9689324-9689343 | MS.gene28158:CDS | 35.0% | |
| GGGGTTATGCAGAAAAATTA+AGG | - | chr4.4:9688738-9688757 | None:intergenic | 35.0% | |
| TTATCTGTAAGCGAAATTGC+AGG | - | chr4.4:9689168-9689187 | None:intergenic | 35.0% | |
| ! | CTTTACACTCAGATTAGTCA+GGG | + | chr4.4:9688930-9688949 | MS.gene28158:intron | 35.0% |
| ! | TCTTTACACTCAGATTAGTC+AGG | + | chr4.4:9688929-9688948 | MS.gene28158:intron | 35.0% |
| ! | TGTTGTTGTTGTTGTACAGT+GGG | + | chr4.4:9689514-9689533 | MS.gene28158:intron | 35.0% |
| ! | TTGTTGTTGTTGTTGTACAG+TGG | + | chr4.4:9689513-9689532 | MS.gene28158:intron | 35.0% |
| ! | TTTTCCTGCTCTTTCTCTTT+TGG | + | chr4.4:9689387-9689406 | MS.gene28158:intron | 35.0% |
| !! | GTCATACACAATGGATTTTG+AGG | + | chr4.4:9689278-9689297 | MS.gene28158:CDS | 35.0% |
| !!! | CTTTTTTTTATGGCTGCACT+TGG | + | chr4.4:9689948-9689967 | MS.gene28158:intron | 35.0% |
| !!! | TTACTGGTTTTTGCAGCAAT+TGG | + | chr4.4:9689905-9689924 | MS.gene28158:intron | 35.0% |
| AACTGAAGAAGATAACGTGG+AGG | - | chr4.4:9689869-9689888 | None:intergenic | 40.0% | |
| AAGAGCCTACTCTTCGTATT+TGG | + | chr4.4:9690076-9690095 | MS.gene28158:CDS | 40.0% | |
| AATACGAAGAGTAGGCTCTT+AGG | - | chr4.4:9690076-9690095 | None:intergenic | 40.0% | |
| ATTCCTGCTTAACAAAGTGC+TGG | - | chr4.4:9689806-9689825 | None:intergenic | 40.0% | |
| CTCTGGTATGTCATACACAA+TGG | + | chr4.4:9689269-9689288 | MS.gene28158:CDS | 40.0% | |
| GAAAGTGATCGAATTGAGAG+AGG | - | chr4.4:9688552-9688571 | None:intergenic | 40.0% | |
| GAGATAAGAAATCACCCATG+TGG | - | chr4.4:9688848-9688867 | None:intergenic | 40.0% | |
| GATCGAATTGAGAGAGGTTT+AGG | - | chr4.4:9688546-9688565 | None:intergenic | 40.0% | |
| GCATTCCAAATACGAAGAGT+AGG | - | chr4.4:9690084-9690103 | None:intergenic | 40.0% | |
| TAAGCTTGGCAAACGAAAGA+AGG | + | chr4.4:9689989-9690008 | MS.gene28158:intron | 40.0% | |
| TAGAAGAATATGGGGCTTAC+CGG | - | chr4.4:9688586-9688605 | None:intergenic | 40.0% | |
| TTAATTAGGGTAGGCAAAGC+GGG | - | chr4.4:9688758-9688777 | None:intergenic | 40.0% | |
| ! | TATTCAATGATGGAGTCGTC+TGG | - | chr4.4:9689321-9689340 | None:intergenic | 40.0% |
| !! | ACACAATGGATTTTGAGGAC+AGG | + | chr4.4:9689283-9689302 | MS.gene28158:CDS | 40.0% |
| !! | AGAATTGGTGTCAAACAAGG+TGG | + | chr4.4:9689243-9689262 | MS.gene28158:CDS | 40.0% |
| !! | CACAATGGATTTTGAGGACA+GGG | + | chr4.4:9689284-9689303 | MS.gene28158:CDS | 40.0% |
| !! | GAATTGGTGTCAAACAAGGT+GGG | + | chr4.4:9689244-9689263 | MS.gene28158:CDS | 40.0% |
| !!! | GTGGGTGTTTTATTTCAACG+CGG | + | chr4.4:9689532-9689551 | MS.gene28158:intron | 40.0% |
| !!! | TGGGTGTTTTATTTCAACGC+GGG | + | chr4.4:9689533-9689552 | MS.gene28158:intron | 40.0% |
| ATACATGGTCGAGACAGACA+CGG | - | chr4.4:9689086-9689105 | None:intergenic | 45.0% | |
| ATTGGAACGCGAACGAATTG+GGG | - | chr4.4:9688465-9688484 | None:intergenic | 45.0% | |
| CAAACCTAGCAGCAAGTACT+GGG | - | chr4.4:9688691-9688710 | None:intergenic | 45.0% | |
| CTAAAGCGGCGATTGAGAAT+TGG | - | chr4.4:9688522-9688541 | None:intergenic | 45.0% | |
| GACTACAGTGATGCTCTGAT+TGG | + | chr4.4:9690109-9690128 | MS.gene28158:CDS | 45.0% | |
| GAGAGAGAGCTATGAGTGAT+TGG | - | chr4.4:9688483-9688502 | None:intergenic | 45.0% | |
| GATTGGAACGCGAACGAATT+GGG | - | chr4.4:9688466-9688485 | None:intergenic | 45.0% | |
| TAATTAGGGTAGGCAAAGCG+GGG | - | chr4.4:9688757-9688776 | None:intergenic | 45.0% | |
| TACAGTGATGCTCTGATTGG+AGG | + | chr4.4:9690112-9690131 | MS.gene28158:CDS | 45.0% | |
| TCACTTTCCGTTATTGCTGC+CGG | + | chr4.4:9688564-9688583 | MS.gene28158:CDS | 45.0% | |
| TGATTGGAACGCGAACGAAT+TGG | - | chr4.4:9688467-9688486 | None:intergenic | 45.0% | |
| TGGACCAAAAGAGAAAGAGC+AGG | - | chr4.4:9689394-9689413 | None:intergenic | 45.0% | |
| ! | AAACCTAGCAGCAAGTACTG+GGG | - | chr4.4:9688690-9688709 | None:intergenic | 45.0% |
| ! | AGAGAGGTTTAGGGCTAAAG+CGG | - | chr4.4:9688536-9688555 | None:intergenic | 45.0% |
| ! | GCTCTTTCTCTTTTGGTCCA+AGG | + | chr4.4:9689394-9689413 | MS.gene28158:intron | 45.0% |
| ! | TCAGGGTTCGTTGTGACTTT+TGG | + | chr4.4:9688947-9688966 | MS.gene28158:intron | 45.0% |
| !! | CTGGTTTTTGCAGCAATTGG+TGG | + | chr4.4:9689908-9689927 | MS.gene28158:intron | 45.0% |
| !! | TTTTAGCTGCACCTGCATCT+GGG | + | chr4.4:9689133-9689152 | MS.gene28158:intron | 45.0% |
| !!! | ATTTTAGCTGCACCTGCATC+TGG | + | chr4.4:9689132-9689151 | MS.gene28158:intron | 45.0% |
| AAGCGAAATTGCAGGTGCCA+CGG | - | chr4.4:9689160-9689179 | None:intergenic | 50.0% | |
| AGTGATGCTCTGATTGGAGG+AGG | + | chr4.4:9690115-9690134 | MS.gene28158:CDS | 50.0% | |
| CAATTACTACTACCGCGCAC+TGG | + | chr4.4:9688436-9688455 | MS.gene28158:CDS | 50.0% | |
| CCAAACCTAGCAGCAAGTAC+TGG | - | chr4.4:9688692-9688711 | None:intergenic | 50.0% | |
| CCACAGGTTTGTGTCGCATT+AGG | - | chr4.4:9690157-9690176 | None:intergenic | 50.0% | |
| CCTAATGCGACACAAACCTG+TGG | + | chr4.4:9690154-9690173 | MS.gene28158:CDS | 50.0% | |
| CTGCCAGCACTTTGTTAAGC+AGG | + | chr4.4:9689800-9689819 | MS.gene28158:intron | 50.0% | |
| TACATGGTCGAGACAGACAC+GGG | - | chr4.4:9689085-9689104 | None:intergenic | 50.0% | |
| TTGAGGACAGGGCTAATGCA+AGG | + | chr4.4:9689295-9689314 | MS.gene28158:CDS | 50.0% | |
| TTTAGCTGCACCTGCATCTG+GGG | + | chr4.4:9689134-9689153 | MS.gene28158:intron | 50.0% | |
| ! | CCAGTACTTGCTGCTAGGTT+TGG | + | chr4.4:9688689-9688708 | MS.gene28158:intron | 50.0% |
| ! | GGGAGTGCAACAAAGCACTT+CGG | + | chr4.4:9689010-9689029 | MS.gene28158:intron | 50.0% |
| AACCACGGACACATGCCACA+TGG | + | chr4.4:9688830-9688849 | MS.gene28158:intron | 55.0% | |
| ACCACGGACACATGCCACAT+GGG | + | chr4.4:9688831-9688850 | MS.gene28158:intron | 55.0% | |
| AGAGAAAGCAGCACCAGCCA+TGG | - | chr4.4:9688415-9688434 | None:intergenic | 55.0% | |
| GCGACACAAACCTGTGGATG+TGG | + | chr4.4:9690160-9690179 | MS.gene28158:CDS | 55.0% | |
| GGCATGTGTCCGTGGTTCAT+AGG | - | chr4.4:9688827-9688846 | None:intergenic | 55.0% | |
| GGGCTTACCGGCAGCAATAA+CGG | - | chr4.4:9688574-9688593 | None:intergenic | 55.0% | |
| GTCAAACAAGGTGGGTGCTC+TGG | + | chr4.4:9689252-9689271 | MS.gene28158:CDS | 55.0% | |
| ! | AGACCCCAGTACTTGCTGCT+AGG | + | chr4.4:9688684-9688703 | MS.gene28158:intron | 55.0% |
| ACCCATGTGGCATGTGTCCG+TGG | - | chr4.4:9688835-9688854 | None:intergenic | 60.0% | |
| GAATTGGGGAGACCAGTGCG+CGG | - | chr4.4:9688451-9688470 | None:intergenic | 60.0% | |
| ! | TGCACCTGCATCTGGGGCCG+TGG | + | chr4.4:9689140-9689159 | MS.gene28158:CDS | 70.0% |
| ! | GGTGCCACGGCCCCAGATGC+AGG | - | chr4.4:9689147-9689166 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 9688408 | 9690207 | 9688408 | ID=MS.gene28158 |
| chr4.4 | mRNA | 9688408 | 9690207 | 9688408 | ID=MS.gene28158.t1;Parent=MS.gene28158 |
| chr4.4 | exon | 9688408 | 9688585 | 9688408 | ID=MS.gene28158.t1.exon1;Parent=MS.gene28158.t1 |
| chr4.4 | CDS | 9688408 | 9688585 | 9688408 | ID=cds.MS.gene28158.t1;Parent=MS.gene28158.t1 |
| chr4.4 | exon | 9689139 | 9689357 | 9689139 | ID=MS.gene28158.t1.exon2;Parent=MS.gene28158.t1 |
| chr4.4 | CDS | 9689139 | 9689357 | 9689139 | ID=cds.MS.gene28158.t1;Parent=MS.gene28158.t1 |
| chr4.4 | exon | 9690065 | 9690207 | 9690065 | ID=MS.gene28158.t1.exon3;Parent=MS.gene28158.t1 |
| chr4.4 | CDS | 9690065 | 9690207 | 9690065 | ID=cds.MS.gene28158.t1;Parent=MS.gene28158.t1 |
| Gene Sequence |
| Protein sequence |
