Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86137.t1 | RHN50183.1 | 90.1 | 151 | 12 | 1 | 1 | 151 | 1 | 148 | 6.60E-69 | 270 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86137.t1 | Q9FL87 | 48.7 | 152 | 73 | 2 | 5 | 151 | 5 | 156 | 4.2e-32 | 139.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86137.t1 | A0A396HC27 | 90.1 | 151 | 12 | 1 | 1 | 151 | 1 | 148 | 4.8e-69 | 270.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene86137 | 0.800625 | 1.32E-48 | -1.69E-46 |
| MS.gene049351 | MS.gene86137 | 0.853365 | 2.51E-61 | -1.69E-46 |
| MS.gene049356 | MS.gene86137 | 0.812296 | 4.55E-51 | -1.69E-46 |
| MS.gene049369 | MS.gene86137 | 0.818245 | 2.17E-52 | -1.69E-46 |
| MS.gene049427 | MS.gene86137 | 0.837007 | 6.70E-57 | -1.69E-46 |
| MS.gene049491 | MS.gene86137 | 0.818596 | 1.81E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene86137 | 0.824725 | 6.91E-54 | -1.69E-46 |
| MS.gene049614 | MS.gene86137 | 0.825637 | 4.20E-54 | -1.69E-46 |
| MS.gene049661 | MS.gene86137 | 0.868873 | 4.72E-66 | -1.69E-46 |
| MS.gene049662 | MS.gene86137 | 0.881004 | 3.44E-70 | -1.69E-46 |
| MS.gene049758 | MS.gene86137 | 0.847935 | 8.44E-60 | -1.69E-46 |
| MS.gene049760 | MS.gene86137 | 0.801747 | 7.78E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene86137 | 0.819723 | 1.00E-52 | -1.69E-46 |
| MS.gene050076 | MS.gene86137 | 0.858099 | 1.04E-62 | -1.69E-46 |
| MS.gene050099 | MS.gene86137 | 0.801275 | 9.72E-49 | -1.69E-46 |
| MS.gene050128 | MS.gene86137 | 0.805619 | 1.22E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene86137 | 0.872292 | 3.56E-67 | -1.69E-46 |
| MS.gene050397 | MS.gene86137 | 0.876547 | 1.28E-68 | -1.69E-46 |
| MS.gene050404 | MS.gene86137 | 0.806695 | 7.26E-50 | -1.69E-46 |
| MS.gene050406 | MS.gene86137 | 0.809392 | 1.94E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene86137 | 0.871665 | 5.75E-67 | -1.69E-46 |
| MS.gene050464 | MS.gene86137 | 0.813161 | 2.94E-51 | -1.69E-46 |
| MS.gene050529 | MS.gene86137 | 0.861947 | 7.17E-64 | -1.69E-46 |
| MS.gene050593 | MS.gene86137 | 0.831088 | 2.03E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene86137 | 0.867671 | 1.15E-65 | -1.69E-46 |
| MS.gene050767 | MS.gene86137 | 0.862079 | 6.53E-64 | -1.69E-46 |
| MS.gene050770 | MS.gene86137 | 0.86657 | 2.59E-65 | -1.69E-46 |
| MS.gene050772 | MS.gene86137 | 0.82418 | 9.28E-54 | -1.69E-46 |
| MS.gene050812 | MS.gene86137 | 0.808459 | 3.06E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene86137 | 0.859222 | 4.80E-63 | -1.69E-46 |
| MS.gene05105 | MS.gene86137 | 0.891909 | 2.58E-74 | -1.69E-46 |
| MS.gene051228 | MS.gene86137 | 0.823937 | 1.06E-53 | -1.69E-46 |
| MS.gene051387 | MS.gene86137 | 0.834287 | 3.27E-56 | -1.69E-46 |
| MS.gene051471 | MS.gene86137 | 0.843977 | 1.01E-58 | -1.69E-46 |
| MS.gene051582 | MS.gene86137 | 0.810212 | 1.29E-50 | -1.69E-46 |
| MS.gene051666 | MS.gene86137 | 0.849407 | 3.30E-60 | -1.69E-46 |
| MS.gene051858 | MS.gene86137 | 0.838502 | 2.77E-57 | -1.69E-46 |
| MS.gene051877 | MS.gene86137 | 0.845722 | 3.40E-59 | -1.69E-46 |
| MS.gene052078 | MS.gene86137 | 0.867885 | 9.84E-66 | -1.69E-46 |
| MS.gene052178 | MS.gene86137 | 0.824301 | 8.69E-54 | -1.69E-46 |
| MS.gene052188 | MS.gene86137 | 0.855012 | 8.39E-62 | -1.69E-46 |
| MS.gene052253 | MS.gene86137 | 0.836812 | 7.52E-57 | -1.69E-46 |
| MS.gene052271 | MS.gene86137 | 0.800483 | 1.41E-48 | -1.69E-46 |
| MS.gene052290 | MS.gene86137 | 0.852334 | 4.94E-61 | -1.69E-46 |
| MS.gene052310 | MS.gene86137 | 0.891983 | 2.41E-74 | -1.69E-46 |
| MS.gene052322 | MS.gene86137 | 0.831671 | 1.46E-55 | -1.69E-46 |
| MS.gene052326 | MS.gene86137 | 0.847583 | 1.05E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene86137 | 0.850802 | 1.34E-60 | -1.69E-46 |
| MS.gene052379 | MS.gene86137 | 0.837956 | 3.83E-57 | -1.69E-46 |
| MS.gene052421 | MS.gene86137 | 0.852722 | 3.83E-61 | -1.69E-46 |
| MS.gene052481 | MS.gene86137 | 0.817013 | 4.11E-52 | -1.69E-46 |
| MS.gene052484 | MS.gene86137 | 0.832868 | 7.39E-56 | -1.69E-46 |
| MS.gene052485 | MS.gene86137 | 0.839486 | 1.54E-57 | -1.69E-46 |
| MS.gene052508 | MS.gene86137 | 0.810385 | 1.18E-50 | -1.69E-46 |
| MS.gene052510 | MS.gene86137 | 0.800722 | 1.26E-48 | -1.69E-46 |
| MS.gene052870 | MS.gene86137 | 0.809281 | 2.04E-50 | -1.69E-46 |
| MS.gene053009 | MS.gene86137 | 0.83391 | 4.07E-56 | -1.69E-46 |
| MS.gene053103 | MS.gene86137 | 0.854514 | 1.17E-61 | -1.69E-46 |
| MS.gene053104 | MS.gene86137 | 0.863774 | 1.96E-64 | -1.69E-46 |
| MS.gene053107 | MS.gene86137 | 0.834526 | 2.85E-56 | -1.69E-46 |
| MS.gene053209 | MS.gene86137 | 0.876946 | 9.32E-69 | -1.69E-46 |
| MS.gene053322 | MS.gene86137 | 0.869264 | 3.53E-66 | -1.69E-46 |
| MS.gene053384 | MS.gene86137 | 0.806354 | 8.57E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene86137 | 0.800877 | 1.17E-48 | -1.69E-46 |
| MS.gene053557 | MS.gene86137 | 0.841322 | 5.10E-58 | -1.69E-46 |
| MS.gene053574 | MS.gene86137 | 0.806505 | 7.96E-50 | -1.69E-46 |
| MS.gene053758 | MS.gene86137 | 0.801072 | 1.07E-48 | -1.69E-46 |
| MS.gene053813 | MS.gene86137 | 0.806296 | 8.81E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene86137 | 0.835424 | 1.69E-56 | -1.69E-46 |
| MS.gene054123 | MS.gene86137 | 0.823132 | 1.63E-53 | -1.69E-46 |
| MS.gene054376 | MS.gene86137 | 0.855657 | 5.45E-62 | -1.69E-46 |
| MS.gene05455 | MS.gene86137 | 0.821703 | 3.51E-53 | -1.69E-46 |
| MS.gene054802 | MS.gene86137 | 0.845702 | 3.44E-59 | -1.69E-46 |
| MS.gene054803 | MS.gene86137 | 0.844002 | 9.90E-59 | -1.69E-46 |
| MS.gene054804 | MS.gene86137 | 0.845866 | 3.11E-59 | -1.69E-46 |
| MS.gene054955 | MS.gene86137 | 0.823835 | 1.12E-53 | -1.69E-46 |
| MS.gene05505 | MS.gene86137 | 0.87526 | 3.54E-68 | -1.69E-46 |
| MS.gene055116 | MS.gene86137 | 0.874957 | 4.50E-68 | -1.69E-46 |
| MS.gene055206 | MS.gene86137 | 0.815341 | 9.72E-52 | -1.69E-46 |
| MS.gene055254 | MS.gene86137 | 0.838147 | 3.42E-57 | -1.69E-46 |
| MS.gene055277 | MS.gene86137 | 0.893555 | 5.64E-75 | -1.69E-46 |
| MS.gene055289 | MS.gene86137 | 0.823546 | 1.31E-53 | -1.69E-46 |
| MS.gene055390 | MS.gene86137 | 0.833091 | 6.51E-56 | -1.69E-46 |
| MS.gene055397 | MS.gene86137 | 0.831227 | 1.88E-55 | -1.69E-46 |
| MS.gene055412 | MS.gene86137 | 0.813575 | 2.39E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene86137 | 0.854377 | 1.28E-61 | -1.69E-46 |
| MS.gene055428 | MS.gene86137 | 0.837093 | 6.37E-57 | -1.69E-46 |
| MS.gene055499 | MS.gene86137 | 0.839172 | 1.86E-57 | -1.69E-46 |
| MS.gene055722 | MS.gene86137 | 0.837005 | 6.71E-57 | -1.69E-46 |
| MS.gene05592 | MS.gene86137 | 0.883904 | 3.02E-71 | -1.69E-46 |
| MS.gene05602 | MS.gene86137 | 0.809815 | 1.57E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene86137 | 0.841753 | 3.93E-58 | -1.69E-46 |
| MS.gene056314 | MS.gene86137 | 0.815059 | 1.12E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene86137 | 0.85068 | 1.45E-60 | -1.69E-46 |
| MS.gene056410 | MS.gene86137 | 0.87422 | 8.01E-68 | -1.69E-46 |
| MS.gene056511 | MS.gene86137 | 0.847944 | 8.39E-60 | -1.69E-46 |
| MS.gene056609 | MS.gene86137 | 0.84625 | 2.44E-59 | -1.69E-46 |
| MS.gene056612 | MS.gene86137 | 0.852161 | 5.53E-61 | -1.69E-46 |
| MS.gene056659 | MS.gene86137 | 0.803275 | 3.77E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene86137 | 0.838352 | 3.03E-57 | -1.69E-46 |
| MS.gene056897 | MS.gene86137 | 0.835063 | 2.09E-56 | -1.69E-46 |
| MS.gene057011 | MS.gene86137 | 0.84407 | 9.49E-59 | -1.69E-46 |
| MS.gene05702 | MS.gene86137 | 0.832705 | 8.11E-56 | -1.69E-46 |
| MS.gene057123 | MS.gene86137 | 0.832112 | 1.14E-55 | -1.69E-46 |
| MS.gene057124 | MS.gene86137 | 0.8119 | 5.55E-51 | -1.69E-46 |
| MS.gene057129 | MS.gene86137 | 0.86032 | 2.24E-63 | -1.69E-46 |
| MS.gene057146 | MS.gene86137 | 0.848605 | 5.51E-60 | -1.69E-46 |
| MS.gene057295 | MS.gene86137 | 0.802763 | 4.81E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene86137 | 0.837653 | 4.58E-57 | -1.69E-46 |
| MS.gene05733 | MS.gene86137 | 0.819766 | 9.77E-53 | -1.69E-46 |
| MS.gene05735 | MS.gene86137 | 0.819618 | 1.06E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene86137 | 0.828192 | 1.03E-54 | -1.69E-46 |
| MS.gene057448 | MS.gene86137 | 0.809882 | 1.52E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene86137 | 0.822562 | 2.22E-53 | -1.69E-46 |
| MS.gene057779 | MS.gene86137 | 0.801461 | 8.91E-49 | -1.69E-46 |
| MS.gene057796 | MS.gene86137 | 0.818944 | 1.50E-52 | -1.69E-46 |
| MS.gene057926 | MS.gene86137 | 0.808303 | 3.31E-50 | -1.69E-46 |
| MS.gene057927 | MS.gene86137 | 0.801232 | 9.92E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene86137 | 0.818516 | 1.88E-52 | -1.69E-46 |
| MS.gene058006 | MS.gene86137 | 0.828691 | 7.81E-55 | -1.69E-46 |
| MS.gene058019 | MS.gene86137 | 0.806843 | 6.75E-50 | -1.69E-46 |
| MS.gene058082 | MS.gene86137 | 0.854438 | 1.23E-61 | -1.69E-46 |
| MS.gene05811 | MS.gene86137 | 0.856349 | 3.42E-62 | -1.69E-46 |
| MS.gene058233 | MS.gene86137 | 0.862782 | 3.97E-64 | -1.69E-46 |
| MS.gene058293 | MS.gene86137 | 0.875346 | 3.31E-68 | -1.69E-46 |
| MS.gene058323 | MS.gene86137 | 0.844982 | 5.40E-59 | -1.69E-46 |
| MS.gene058365 | MS.gene86137 | 0.840935 | 6.44E-58 | -1.69E-46 |
| MS.gene058376 | MS.gene86137 | 0.807305 | 5.39E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene86137 | 0.894649 | 2.02E-75 | -1.69E-46 |
| MS.gene058723 | MS.gene86137 | 0.801415 | 9.10E-49 | -1.69E-46 |
| MS.gene058787 | MS.gene86137 | 0.84183 | 3.75E-58 | -1.69E-46 |
| MS.gene058845 | MS.gene86137 | 0.802789 | 4.75E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene86137 | 0.820972 | 5.17E-53 | -1.69E-46 |
| MS.gene059029 | MS.gene86137 | 0.814735 | 1.32E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene86137 | 0.862972 | 3.47E-64 | -1.69E-46 |
| MS.gene059294 | MS.gene86137 | 0.83227 | 1.04E-55 | -1.69E-46 |
| MS.gene059326 | MS.gene86137 | 0.842943 | 1.90E-58 | -1.69E-46 |
| MS.gene059342 | MS.gene86137 | 0.813247 | 2.82E-51 | -1.69E-46 |
| MS.gene05934 | MS.gene86137 | 0.82327 | 1.52E-53 | -1.69E-46 |
| MS.gene059366 | MS.gene86137 | 0.818705 | 1.70E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene86137 | 0.831001 | 2.14E-55 | -1.69E-46 |
| MS.gene059536 | MS.gene86137 | 0.860842 | 1.56E-63 | -1.69E-46 |
| MS.gene059597 | MS.gene86137 | 0.831762 | 1.39E-55 | -1.69E-46 |
| MS.gene05959 | MS.gene86137 | 0.813405 | 2.60E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene86137 | 0.862555 | 4.67E-64 | -1.69E-46 |
| MS.gene059688 | MS.gene86137 | 0.900954 | 4.37E-78 | -1.69E-46 |
| MS.gene059692 | MS.gene86137 | 0.907766 | 3.55E-81 | -1.69E-46 |
| MS.gene059695 | MS.gene86137 | 0.907793 | 3.45E-81 | -1.69E-46 |
| MS.gene059708 | MS.gene86137 | 0.815122 | 1.09E-51 | -1.69E-46 |
| MS.gene059711 | MS.gene86137 | 0.82263 | 2.14E-53 | -1.69E-46 |
| MS.gene059807 | MS.gene86137 | 0.817574 | 3.07E-52 | -1.69E-46 |
| MS.gene05984 | MS.gene86137 | 0.845953 | 2.94E-59 | -1.69E-46 |
| MS.gene059894 | MS.gene86137 | 0.848382 | 6.35E-60 | -1.69E-46 |
| MS.gene059902 | MS.gene86137 | 0.840096 | 1.07E-57 | -1.69E-46 |
| MS.gene059960 | MS.gene86137 | 0.868267 | 7.41E-66 | -1.69E-46 |
| MS.gene059974 | MS.gene86137 | 0.825438 | 4.69E-54 | -1.69E-46 |
| MS.gene060005 | MS.gene86137 | 0.800138 | 1.66E-48 | -1.69E-46 |
| MS.gene060193 | MS.gene86137 | 0.800943 | 1.14E-48 | -1.69E-46 |
| MS.gene06037 | MS.gene86137 | 0.855493 | 6.08E-62 | -1.69E-46 |
| MS.gene060403 | MS.gene86137 | 0.831423 | 1.68E-55 | -1.69E-46 |
| MS.gene060804 | MS.gene86137 | 0.889165 | 3.09E-73 | -1.69E-46 |
| MS.gene060818 | MS.gene86137 | 0.85929 | 4.58E-63 | -1.69E-46 |
| MS.gene060835 | MS.gene86137 | 0.801325 | 9.50E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene86137 | 0.840969 | 6.31E-58 | -1.69E-46 |
| MS.gene06090 | MS.gene86137 | 0.819686 | 1.02E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene86137 | 0.812511 | 4.09E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene86137 | 0.862602 | 4.51E-64 | -1.69E-46 |
| MS.gene061049 | MS.gene86137 | 0.862968 | 3.48E-64 | -1.69E-46 |
| MS.gene061051 | MS.gene86137 | -0.860683 | 1.74E-63 | -1.69E-46 |
| MS.gene061154 | MS.gene86137 | 0.844113 | 9.25E-59 | -1.69E-46 |
| MS.gene061200 | MS.gene86137 | 0.859639 | 3.60E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86137.t1 | MTR_6g015250 | 87.121 | 132 | 14 | 1 | 1 | 132 | 1 | 129 | 5.62e-81 | 235 |
| MS.gene86137.t1 | MTR_6g015275 | 87.786 | 131 | 13 | 1 | 1 | 131 | 1 | 128 | 6.63e-80 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86137.t1 | AT5G39210 | 48.077 | 156 | 76 | 2 | 1 | 151 | 1 | 156 | 2.06e-43 | 140 |
Find 28 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGAGTGGCTCACAAAT+TGG | 0.283330 | 6.3:+9390989 | MS.gene86137:CDS |
| AAGAACTAAAACATTGAATT+AGG | 0.310142 | 6.3:-9389845 | None:intergenic |
| GGTGATGTTATATGTATTGC+TGG | 0.342148 | 6.3:-9389819 | None:intergenic |
| GGATGAGAGGTTTGTGTCAG+AGG | 0.390149 | 6.3:+9390948 | MS.gene86137:CDS |
| CTTGAAATTGATGGAGAAGT+TGG | 0.397574 | 6.3:+9391096 | MS.gene86137:CDS |
| ATCAATTTCAAGTTCACAAA+AGG | 0.404929 | 6.3:-9391085 | None:intergenic |
| GTTTCATATCTTGCTAGATC+AGG | 0.459128 | 6.3:-9391030 | None:intergenic |
| TCAGTGGTATGAAGTTCGTT+TGG | 0.464080 | 6.3:+9391125 | MS.gene86137:CDS |
| CAACTTGAGGCAGTCTATGA+TGG | 0.478137 | 6.3:-9389872 | None:intergenic |
| GTATTGCTGGATTCTGCATA+TGG | 0.507197 | 6.3:-9389806 | None:intergenic |
| ACAAACCTCTCATCCTTCCC+TGG | 0.513237 | 6.3:-9390940 | None:intergenic |
| GAAAGAAACAGCATCATCAA+TGG | 0.526687 | 6.3:-9391052 | None:intergenic |
| TGAAAGATACTTTGAAAGAG+TGG | 0.529707 | 6.3:+9390977 | MS.gene86137:CDS |
| CTAAAACATTGAATTAGGCC+TGG | 0.534335 | 6.3:-9389840 | None:intergenic |
| TCAGGAGGAAGAGTCTTGCC+AGG | 0.535490 | 6.3:-9391012 | None:intergenic |
| GAGTGGCTCACAAATTGGCC+TGG | 0.539314 | 6.3:+9390994 | MS.gene86137:CDS |
| TCATATCTTGCTAGATCAGG+AGG | 0.578859 | 6.3:-9391027 | None:intergenic |
| TAGAACCAGGGAAGGATGAG+AGG | 0.587726 | 6.3:+9390935 | MS.gene86137:CDS |
| AATTGATGGAGAAGTTGGAT+CGG | 0.615121 | 6.3:+9391101 | MS.gene86137:CDS |
| ACTTATGTACTATTAGAACC+AGG | 0.616078 | 6.3:+9390922 | MS.gene86137:CDS |
| CTGCAATCGAGTGCAACTTG+AGG | 0.629225 | 6.3:-9389885 | None:intergenic |
| TGTACTATTAGAACCAGGGA+AGG | 0.630431 | 6.3:+9390927 | MS.gene86137:CDS |
| GAGAAGTTGGATCGGTTCAG+TGG | 0.633875 | 6.3:+9391109 | MS.gene86137:CDS |
| GCAATACATATAACATCACC+AGG | 0.652099 | 6.3:+9389822 | MS.gene86137:CDS |
| CTTATGTACTATTAGAACCA+GGG | 0.664769 | 6.3:+9390923 | MS.gene86137:CDS |
| AGGCTGCAAAACAACTACCA+TGG | 0.682488 | 6.3:-9389272 | None:intergenic |
| CGATTGCAGAAGTAAAACAA+AGG | 0.695066 | 6.3:+9389899 | MS.gene86137:CDS |
| AGTCTAACATTGAAACCATG+AGG | 0.697168 | 6.3:-9389292 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTCTTATATTTAGTTTTA+AGG | + | chr6.3:9389990-9390009 | MS.gene86137:intron | 10.0% |
| !!! | GTTAGTTTTTTATTTATTTT+TGG | - | chr6.3:9390753-9390772 | None:intergenic | 10.0% |
| !! | AATAAATAAATAAATCAACC+AGG | + | chr6.3:9390121-9390140 | MS.gene86137:intron | 15.0% |
| !!! | ATACATTACATTATTTTTAG+AGG | + | chr6.3:9389404-9389423 | MS.gene86137:intron | 15.0% |
| !!! | TACATTACATTATTTTTAGA+GGG | + | chr6.3:9389405-9389424 | MS.gene86137:intron | 15.0% |
| !! | AAGAACTAAAACATTGAATT+AGG | - | chr6.3:9389848-9389867 | None:intergenic | 20.0% |
| !! | ATTATCAATACTAGTAAGTA+GGG | + | chr6.3:9389452-9389471 | MS.gene86137:intron | 20.0% |
| !! | TATTATCAATACTAGTAAGT+AGG | + | chr6.3:9389451-9389470 | MS.gene86137:intron | 20.0% |
| !! | TTAGTCAAAACTAATTTACT+CGG | + | chr6.3:9390518-9390537 | MS.gene86137:intron | 20.0% |
| !!! | ATTCTTGTGATTAGTTAATA+GGG | + | chr6.3:9390152-9390171 | MS.gene86137:intron | 20.0% |
| !!! | GTTTTTTGAAAACATACTAT+AGG | - | chr6.3:9389959-9389978 | None:intergenic | 20.0% |
| !!! | TATTCTTGTGATTAGTTAAT+AGG | + | chr6.3:9390151-9390170 | MS.gene86137:intron | 20.0% |
| ! | AAACATTCATACCAAGATTA+TGG | - | chr6.3:9389695-9389714 | None:intergenic | 25.0% |
| ! | CATTTGTATAATTCCTTAGA+TGG | + | chr6.3:9389479-9389498 | MS.gene86137:intron | 25.0% |
| ! | TAAACAATTGACCATAATCT+TGG | + | chr6.3:9389681-9389700 | MS.gene86137:intron | 25.0% |
| ! | TGAATGATTGTAAAATAGAG+TGG | + | chr6.3:9389577-9389596 | MS.gene86137:intron | 25.0% |
| !! | TTTTGTGAACTTGAAATTGA+TGG | + | chr6.3:9391087-9391106 | MS.gene86137:CDS | 25.0% |
| !!! | ATCAATTTCAAGTTCACAAA+AGG | - | chr6.3:9391088-9391107 | None:intergenic | 25.0% |
| !!! | GTTTTGACTAATCATATGTT+TGG | - | chr6.3:9390509-9390528 | None:intergenic | 25.0% |
| ACTTATGTACTATTAGAACC+AGG | + | chr6.3:9390922-9390941 | MS.gene86137:CDS | 30.0% | |
| AGGTTAAATGTGATAGTAAC+AGG | + | chr6.3:9390570-9390589 | MS.gene86137:intron | 30.0% | |
| CAGAAAATTCCTATATGGTA+AGG | - | chr6.3:9389514-9389533 | None:intergenic | 30.0% | |
| CAGTCGAGATTCAAATATTA+GGG | - | chr6.3:9390656-9390675 | None:intergenic | 30.0% | |
| CTAATATTTGAATCTCGACT+GGG | + | chr6.3:9390655-9390674 | MS.gene86137:intron | 30.0% | |
| CTTATGTACTATTAGAACCA+GGG | + | chr6.3:9390923-9390942 | MS.gene86137:CDS | 30.0% | |
| GTTTCTTTCCATCAAGTTAA+GGG | - | chr6.3:9389537-9389556 | None:intergenic | 30.0% | |
| TGCTCCTAAGAAATGTTATA+TGG | + | chr6.3:9390546-9390565 | MS.gene86137:intron | 30.0% | |
| TGTTTCTTTCCATCAAGTTA+AGG | - | chr6.3:9389538-9389557 | None:intergenic | 30.0% | |
| ! | GATTAGTTAATAGGGTTCTT+TGG | + | chr6.3:9390160-9390179 | MS.gene86137:intron | 30.0% |
| ! | TGAAAGATACTTTGAAAGAG+TGG | + | chr6.3:9390977-9390996 | MS.gene86137:CDS | 30.0% |
| !! | CTTAACTTTCTCTGAAATCA+GGG | - | chr6.3:9390201-9390220 | None:intergenic | 30.0% |
| AAACACACACTTAGCATGTT+TGG | + | chr6.3:9390359-9390378 | MS.gene86137:intron | 35.0% | |
| AAACATGCTAAGTGTGTGTT+TGG | - | chr6.3:9390360-9390379 | None:intergenic | 35.0% | |
| AAGGGCAGAAAATTCCTATA+TGG | - | chr6.3:9389519-9389538 | None:intergenic | 35.0% | |
| AATTGATGGAGAAGTTGGAT+CGG | + | chr6.3:9391101-9391120 | MS.gene86137:CDS | 35.0% | |
| AGATTTCAGAAGATACTGCA+GGG | - | chr6.3:9389931-9389950 | None:intergenic | 35.0% | |
| AGCCATAACATTGAGGTTAT+TGG | + | chr6.3:9389641-9389660 | MS.gene86137:intron | 35.0% | |
| AGTCTAACATTGAAACCATG+AGG | - | chr6.3:9389295-9389314 | None:intergenic | 35.0% | |
| AGTTAAGCATGTGTTTGGTA+TGG | + | chr6.3:9390214-9390233 | MS.gene86137:intron | 35.0% | |
| CCAAAATTGCCTTACCATAT+AGG | + | chr6.3:9389502-9389521 | MS.gene86137:intron | 35.0% | |
| CCAGTCGAGATTCAAATATT+AGG | - | chr6.3:9390657-9390676 | None:intergenic | 35.0% | |
| CCTAAGAAATGTTATATGGC+AGG | + | chr6.3:9390550-9390569 | MS.gene86137:intron | 35.0% | |
| CCTAATATTTGAATCTCGAC+TGG | + | chr6.3:9390654-9390673 | MS.gene86137:intron | 35.0% | |
| CCTGCCATATAACATTTCTT+AGG | - | chr6.3:9390553-9390572 | None:intergenic | 35.0% | |
| CGATTGCAGAAGTAAAACAA+AGG | + | chr6.3:9389899-9389918 | MS.gene86137:CDS | 35.0% | |
| CTAAAACATTGAATTAGGCC+TGG | - | chr6.3:9389843-9389862 | None:intergenic | 35.0% | |
| CTCCAATAACCTCAATGTTA+TGG | - | chr6.3:9389646-9389665 | None:intergenic | 35.0% | |
| CTTGAAATTGATGGAGAAGT+TGG | + | chr6.3:9391096-9391115 | MS.gene86137:CDS | 35.0% | |
| GAAAGAAACAGCATCATCAA+TGG | - | chr6.3:9391055-9391074 | None:intergenic | 35.0% | |
| GAGAAAGTTAAGCATGTGTT+TGG | + | chr6.3:9390209-9390228 | MS.gene86137:intron | 35.0% | |
| GCAATACATATAACATCACC+AGG | + | chr6.3:9389822-9389841 | MS.gene86137:CDS | 35.0% | |
| GCTCAAAAGCTGAAAAGATA+AGG | - | chr6.3:9389348-9389367 | None:intergenic | 35.0% | |
| GGTGATGTTATATGTATTGC+TGG | - | chr6.3:9389822-9389841 | None:intergenic | 35.0% | |
| GTTTCATATCTTGCTAGATC+AGG | - | chr6.3:9391033-9391052 | None:intergenic | 35.0% | |
| TGTGTGTTTGGCAAAAAGTT+TGG | - | chr6.3:9390348-9390367 | None:intergenic | 35.0% | |
| TTGACGTGGAAGATTTGATT+TGG | + | chr6.3:9390421-9390440 | MS.gene86137:intron | 35.0% | |
| ! | CACTGAACTTTGTTTGTAAC+AGG | + | chr6.3:9390858-9390877 | MS.gene86137:intron | 35.0% |
| ! | CCTATATGGTAAGGCAATTT+TGG | - | chr6.3:9389505-9389524 | None:intergenic | 35.0% |
| ! | TGTAACAGGTTTTTGCAATG+AGG | + | chr6.3:9390872-9390891 | MS.gene86137:intron | 35.0% |
| ! | TTGGAGCTTTGATGAATTTG+CGG | + | chr6.3:9390440-9390459 | MS.gene86137:intron | 35.0% |
| ! | TTTTGCAATGAGGAGAAGAA+GGG | + | chr6.3:9390882-9390901 | MS.gene86137:CDS | 35.0% |
| ! | TTTTTGCAATGAGGAGAAGA+AGG | + | chr6.3:9390881-9390900 | MS.gene86137:CDS | 35.0% |
| !! | ATTTTCTGCCCTTAACTTGA+TGG | + | chr6.3:9389526-9389545 | MS.gene86137:intron | 35.0% |
| !! | CTCCACTGTAAGCTATTTTA+TGG | + | chr6.3:9389322-9389341 | MS.gene86137:intron | 35.0% |
| !! | GCTTAACTTTCTCTGAAATC+AGG | - | chr6.3:9390202-9390221 | None:intergenic | 35.0% |
| AATAGGATTACCTCTCGTGA+AGG | + | chr6.3:9390713-9390732 | MS.gene86137:intron | 40.0% | |
| ACGAGAGGTAATCCTATTTG+AGG | - | chr6.3:9390711-9390730 | None:intergenic | 40.0% | |
| ATAGGATTACCTCTCGTGAA+GGG | + | chr6.3:9390714-9390733 | MS.gene86137:intron | 40.0% | |
| CAAATGCAGCCATAACATTG+AGG | + | chr6.3:9389634-9389653 | MS.gene86137:intron | 40.0% | |
| CGATGAAAATCATGTTGACG+TGG | + | chr6.3:9390407-9390426 | MS.gene86137:intron | 40.0% | |
| CGTTGGAACTTGCAAAATCA+TGG | + | chr6.3:9390237-9390256 | MS.gene86137:intron | 40.0% | |
| GAGATTTCAGAAGATACTGC+AGG | - | chr6.3:9389932-9389951 | None:intergenic | 40.0% | |
| GCACAGTTGGATAATAAACG+CGG | - | chr6.3:9390689-9390708 | None:intergenic | 40.0% | |
| GGCCATAAAATAGCTTACAG+TGG | - | chr6.3:9389327-9389346 | None:intergenic | 40.0% | |
| GTATTGCTGGATTCTGCATA+TGG | - | chr6.3:9389809-9389828 | None:intergenic | 40.0% | |
| TATCCAACTGTGCCTCAAAT+AGG | + | chr6.3:9390696-9390715 | MS.gene86137:intron | 40.0% | |
| TCACAAGAATACTTAGAGCC+TGG | - | chr6.3:9390142-9390161 | None:intergenic | 40.0% | |
| TCAGTGGTATGAAGTTCGTT+TGG | + | chr6.3:9391125-9391144 | MS.gene86137:CDS | 40.0% | |
| TCATATCTTGCTAGATCAGG+AGG | - | chr6.3:9391030-9391049 | None:intergenic | 40.0% | |
| TGAAAGAGTGGCTCACAAAT+TGG | + | chr6.3:9390989-9391008 | MS.gene86137:CDS | 40.0% | |
| TGGAACTTGCAAAATCATGG+TGG | + | chr6.3:9390240-9390259 | MS.gene86137:intron | 40.0% | |
| TGTACTATTAGAACCAGGGA+AGG | + | chr6.3:9390927-9390946 | MS.gene86137:CDS | 40.0% | |
| ! | AATCCTATTTGAGGCACAGT+TGG | - | chr6.3:9390702-9390721 | None:intergenic | 40.0% |
| ! | CATGGTGGAAAATCACGTTT+GGG | + | chr6.3:9390255-9390274 | MS.gene86137:intron | 40.0% |
| ! | TCATGGTGGAAAATCACGTT+TGG | + | chr6.3:9390254-9390273 | MS.gene86137:intron | 40.0% |
| ! | TGTTATGGCTGCATTTGCTA+TGG | - | chr6.3:9389631-9389650 | None:intergenic | 40.0% |
| !! | AAGGCAATTTTGGCCATCTA+AGG | - | chr6.3:9389495-9389514 | None:intergenic | 40.0% |
| !! | ACTTAGCATGTTTGGTTCTG+CGG | + | chr6.3:9390367-9390386 | MS.gene86137:intron | 40.0% |
| !! | ACTTTCTCTGAAATCAGGGT+TGG | - | chr6.3:9390197-9390216 | None:intergenic | 40.0% |
| AAGCCCAAAACCAATTCCCA+AGG | - | chr6.3:9390325-9390344 | None:intergenic | 45.0% | |
| AGAGCTAATCTTTCACAGCG+TGG | + | chr6.3:9390627-9390646 | MS.gene86137:intron | 45.0% | |
| ATTTGAATCTCGACTGGGAG+AGG | + | chr6.3:9390660-9390679 | MS.gene86137:intron | 45.0% | |
| CAACTTGAGGCAGTCTATGA+TGG | - | chr6.3:9389875-9389894 | None:intergenic | 45.0% | |
| CTACATATTCCCTTCACGAG+AGG | - | chr6.3:9390726-9390745 | None:intergenic | 45.0% | |
| TTCAAGTGCCAACCATGGTT+AGG | + | chr6.3:9390594-9390613 | MS.gene86137:intron | 45.0% | |
| TTCCAGCTTCAGCCAAATCA+CGG | + | chr6.3:9390291-9390310 | MS.gene86137:intron | 45.0% | |
| ! | AAGGCACTTTCACCGTGATT+TGG | - | chr6.3:9390306-9390325 | None:intergenic | 45.0% |
| ! | ACTAACTGTGTCAAGTGCTG+TGG | + | chr6.3:9390767-9390786 | MS.gene86137:intron | 45.0% |
| !! | AAATCACGGTGAAAGTGCCT+TGG | + | chr6.3:9390305-9390324 | MS.gene86137:intron | 45.0% |
| !! | AATCACGGTGAAAGTGCCTT+GGG | + | chr6.3:9390306-9390325 | MS.gene86137:intron | 45.0% |
| !! | AGTGCCTTGGGAATTGGTTT+TGG | + | chr6.3:9390318-9390337 | MS.gene86137:intron | 45.0% |
| !! | CTGAAATCAGGGTTGGTACT+TGG | - | chr6.3:9390190-9390209 | None:intergenic | 45.0% |
| !! | CTTAGCATGTTTGGTTCTGC+GGG | + | chr6.3:9390368-9390387 | MS.gene86137:intron | 45.0% |
| !! | GTAGTTGTTTTGCAGCCTCA+TGG | + | chr6.3:9389277-9389296 | MS.gene86137:CDS | 45.0% |
| !! | TTAGCATGTTTGGTTCTGCG+GGG | + | chr6.3:9390369-9390388 | MS.gene86137:intron | 45.0% |
| !!! | GTGCCTTGGGAATTGGTTTT+GGG | + | chr6.3:9390319-9390338 | MS.gene86137:intron | 45.0% |
| ACAAACCTCTCATCCTTCCC+TGG | - | chr6.3:9390943-9390962 | None:intergenic | 50.0% | |
| CCAACCATGGTTAGGTCTAG+TGG | + | chr6.3:9390602-9390621 | MS.gene86137:intron | 50.0% | |
| CCACTAGACCTAACCATGGT+TGG | - | chr6.3:9390605-9390624 | None:intergenic | 50.0% | |
| CTCTCCACTAGACCTAACCA+TGG | - | chr6.3:9390609-9390628 | None:intergenic | 50.0% | |
| CTGCAATCGAGTGCAACTTG+AGG | - | chr6.3:9389888-9389907 | None:intergenic | 50.0% | |
| GCATGTGTTTGGTATGGCGT+TGG | + | chr6.3:9390220-9390239 | MS.gene86137:intron | 50.0% | |
| GGATGAGAGGTTTGTGTCAG+AGG | + | chr6.3:9390948-9390967 | MS.gene86137:CDS | 50.0% | |
| TAGAACCAGGGAAGGATGAG+AGG | + | chr6.3:9390935-9390954 | MS.gene86137:CDS | 50.0% | |
| ! | CAGGTTTCAAGTGCCAACCA+TGG | + | chr6.3:9390589-9390608 | MS.gene86137:intron | 50.0% |
| ! | GAGAAGTTGGATCGGTTCAG+TGG | + | chr6.3:9391109-9391128 | MS.gene86137:CDS | 50.0% |
| ! | TGTGTCAAGTGCTGTGGCTT+TGG | + | chr6.3:9390773-9390792 | MS.gene86137:intron | 50.0% |
| !! | GGTGAAAGTGCCTTGGGAAT+TGG | + | chr6.3:9390312-9390331 | MS.gene86137:intron | 50.0% |
| GAGTGGCTCACAAATTGGCC+TGG | + | chr6.3:9390994-9391013 | MS.gene86137:CDS | 55.0% | |
| TCAGGAGGAAGAGTCTTGCC+AGG | - | chr6.3:9391015-9391034 | None:intergenic | 55.0% | |
| ! | CACCGTGATTTGGCTGAAGC+TGG | - | chr6.3:9390296-9390315 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 9389274 | 9391152 | 9389274 | ID=MS.gene86137 |
| chr6.3 | mRNA | 9389274 | 9391152 | 9389274 | ID=MS.gene86137.t1;Parent=MS.gene86137 |
| chr6.3 | exon | 9389274 | 9389328 | 9389274 | ID=MS.gene86137.t1.exon1;Parent=MS.gene86137.t1 |
| chr6.3 | CDS | 9389274 | 9389328 | 9389274 | ID=cds.MS.gene86137.t1;Parent=MS.gene86137.t1 |
| chr6.3 | exon | 9389793 | 9389920 | 9389793 | ID=MS.gene86137.t1.exon2;Parent=MS.gene86137.t1 |
| chr6.3 | CDS | 9389793 | 9389920 | 9389793 | ID=cds.MS.gene86137.t1;Parent=MS.gene86137.t1 |
| chr6.3 | exon | 9390880 | 9391152 | 9390880 | ID=MS.gene86137.t1.exon3;Parent=MS.gene86137.t1 |
| chr6.3 | CDS | 9390880 | 9391152 | 9390880 | ID=cds.MS.gene86137.t1;Parent=MS.gene86137.t1 |
| Gene Sequence |
| Protein sequence |
