Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0053240 | XP_003589004.1 | 86.179 | 246 | 22 | 2 | 56 | 289 | 19 | 264 | 2.18e-117 | 350 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0053240 | G7I9Z4 | 86.179 | 246 | 22 | 2 | 56 | 289 | 19 | 264 | 1.04e-117 | 350 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006880 | Msa0053240 | 0.811619 | 6.393737e-51 | -8.615850e-47 |
| Msa0007850 | Msa0053240 | 0.828280 | 9.812535e-55 | -8.615850e-47 |
| Msa0029630 | Msa0053240 | 0.802644 | 5.086323e-49 | -8.615850e-47 |
| Msa0038000 | Msa0053240 | 0.805134 | 1.545311e-49 | -8.615850e-47 |
| Msa0053240 | Msa0056270 | 0.807021 | 6.194129e-50 | -8.615850e-47 |
| Msa0053240 | Msa0070430 | 0.805757 | 1.144010e-49 | -8.615850e-47 |
| Msa0053240 | Msa0097950 | 0.814654 | 1.380179e-51 | -8.615850e-47 |
| Msa0053240 | Msa0098740 | 0.921102 | 5.498671e-88 | -8.615850e-47 |
| Msa0053240 | Msa0108870 | 0.806124 | 9.577137e-50 | -8.615850e-47 |
| Msa0053240 | Msa0125450 | 0.806982 | 6.313075e-50 | -8.615850e-47 |
| Msa0053240 | Msa0125540 | 0.802758 | 4.819606e-49 | -8.615850e-47 |
| Msa0053240 | Msa0131590 | 0.812475 | 4.161549e-51 | -8.615850e-47 |
| Msa0053240 | Msa0166750 | 0.806437 | 8.230527e-50 | -8.615850e-47 |
| Msa0053240 | Msa0177230 | 0.808605 | 2.853344e-50 | -8.615850e-47 |
| Msa0053240 | Msa0180020 | 0.808736 | 2.675166e-50 | -8.615850e-47 |
| Msa0053240 | Msa0198520 | 0.801146 | 1.033398e-48 | -8.615850e-47 |
| Msa0053240 | Msa0198530 | 0.814585 | 1.429385e-51 | -8.615850e-47 |
| Msa0053240 | Msa0259160 | 0.801862 | 7.368786e-49 | -8.615850e-47 |
| Msa0053240 | Msa0267870 | 0.811914 | 5.514889e-51 | -8.615850e-47 |
| Msa0053240 | Msa0291520 | 0.803521 | 3.350465e-49 | -8.615850e-47 |
| Msa0053240 | Msa0295590 | 0.812300 | 4.543523e-51 | -8.615850e-47 |
| Msa0053240 | Msa0363620 | 0.817755 | 2.797650e-52 | -8.615850e-47 |
| Msa0053240 | Msa0416400 | 0.802828 | 4.660672e-49 | -8.615850e-47 |
| Msa0053240 | Msa0419520 | 0.832327 | 1.006951e-55 | -8.615850e-47 |
| Msa0053240 | Msa0434770 | 0.802734 | 4.874737e-49 | -8.615850e-47 |
| Msa0053240 | Msa0443390 | 0.801248 | 9.849333e-49 | -8.615850e-47 |
| Msa0053240 | Msa0455380 | 0.816351 | 5.782725e-52 | -8.615850e-47 |
| Msa0053240 | Msa0465550 | 0.805045 | 1.613340e-49 | -8.615850e-47 |
| Msa0053240 | Msa0474700 | 0.800850 | 1.187866e-48 | -8.615850e-47 |
| Msa0053240 | Msa0478580 | 0.830700 | 2.534244e-55 | -8.615850e-47 |
| Msa0053240 | Msa0484290 | 0.821529 | 3.845871e-53 | -8.615850e-47 |
| Msa0053240 | Msa0550540 | 0.820129 | 8.074422e-53 | -8.615850e-47 |
| Msa0053240 | Msa0550570 | 0.823914 | 1.071616e-53 | -8.615850e-47 |
| Msa0053240 | Msa0593700 | 0.811268 | 7.620733e-51 | -8.615850e-47 |
| Msa0053240 | Msa0603570 | 0.802128 | 6.497960e-49 | -8.615850e-47 |
| Msa0053240 | Msa0609000 | 0.820972 | 5.171984e-53 | -8.615850e-47 |
| Msa0053240 | Msa0610310 | 0.819634 | 1.047812e-52 | -8.615850e-47 |
| Msa0053240 | Msa0618930 | 0.816759 | 4.686653e-52 | -8.615850e-47 |
| Msa0053240 | Msa0633570 | 0.808395 | 3.162634e-50 | -8.615850e-47 |
| Msa0053240 | Msa0654870 | 0.806080 | 9.783752e-50 | -8.615850e-47 |
| Msa0053240 | Msa0658480 | 0.803026 | 4.242678e-49 | -8.615850e-47 |
| Msa0053240 | Msa0659990 | 0.805476 | 1.310725e-49 | -8.615850e-47 |
| Msa0053240 | Msa0666230 | 0.812232 | 4.702621e-51 | -8.615850e-47 |
| Msa0053240 | Msa0683780 | 0.821261 | 4.435200e-53 | -8.615850e-47 |
| Msa0053240 | Msa0687230 | 0.818131 | 2.299934e-52 | -8.615850e-47 |
| Msa0053240 | Msa0694990 | 0.817338 | 3.473343e-52 | -8.615850e-47 |
| Msa0053240 | Msa0699190 | 0.813165 | 2.938353e-51 | -8.615850e-47 |
| Msa0053240 | Msa0704660 | 0.802847 | 4.618987e-49 | -8.615850e-47 |
| Msa0053240 | Msa0704800 | 0.809331 | 1.994716e-50 | -8.615850e-47 |
| Msa0053240 | Msa0706390 | 0.807443 | 5.041858e-50 | -8.615850e-47 |
| Msa0053240 | Msa0707390 | 0.820488 | 6.680704e-53 | -8.615850e-47 |
| Msa0053240 | Msa0734960 | 0.814699 | 1.348947e-51 | -8.615850e-47 |
| Msa0053240 | Msa0744090 | 0.813056 | 3.105579e-51 | -8.615850e-47 |
| Msa0053240 | Msa0744100 | 0.805133 | 1.546249e-49 | -8.615850e-47 |
| Msa0053240 | Msa0774750 | 0.807091 | 5.986671e-50 | -8.615850e-47 |
| Msa0053240 | Msa0781270 | 0.835252 | 1.869660e-56 | -8.615850e-47 |
| Msa0053240 | Msa0788120 | 0.801777 | 7.673314e-49 | -8.615850e-47 |
| Msa0053240 | Msa0795660 | 0.816742 | 4.727716e-52 | -8.615850e-47 |
| Msa0053240 | Msa0818770 | 0.820539 | 6.500833e-53 | -8.615850e-47 |
| Msa0053240 | Msa0823090 | 0.803933 | 2.751753e-49 | -8.615850e-47 |
| Msa0053240 | Msa0824640 | 0.823578 | 1.284194e-53 | -8.615850e-47 |
| Msa0053240 | Msa0825270 | 0.819163 | 1.341720e-52 | -8.615850e-47 |
| Msa0053240 | Msa0825280 | 0.812043 | 5.170641e-51 | -8.615850e-47 |
| Msa0053240 | Msa0839200 | 0.810905 | 9.135949e-51 | -8.615850e-47 |
| Msa0053240 | Msa0851470 | 0.804898 | 1.731957e-49 | -8.615850e-47 |
| Msa0053240 | Msa0858440 | 0.812951 | 3.273867e-51 | -8.615850e-47 |
| Msa0053240 | Msa0864010 | 0.818220 | 2.196143e-52 | -8.615850e-47 |
| Msa0053240 | Msa0888940 | 0.801361 | 9.336857e-49 | -8.615850e-47 |
| Msa0053240 | Msa0897400 | 0.807851 | 4.129769e-50 | -8.615850e-47 |
| Msa0053240 | Msa0943170 | 0.815075 | 1.113442e-51 | -8.615850e-47 |
| Msa0053240 | Msa0960830 | 0.820880 | 5.430201e-53 | -8.615850e-47 |
| Msa0053240 | Msa0963020 | 0.854817 | 9.558799e-62 | -8.615850e-47 |
| Msa0053240 | Msa0966270 | 0.818407 | 1.991936e-52 | -8.615850e-47 |
| Msa0053240 | Msa0973320 | 0.815998 | 6.936565e-52 | -8.615850e-47 |
| Msa0053240 | Msa0981430 | 0.800010 | 1.761554e-48 | -8.615850e-47 |
| Msa0053240 | Msa1017510 | 0.801140 | 1.036373e-48 | -8.615850e-47 |
| Msa0053240 | Msa1019240 | 0.801551 | 8.535565e-49 | -8.615850e-47 |
| Msa0053240 | Msa1025940 | 0.864685 | 1.017738e-64 | -8.615850e-47 |
| Msa0053240 | Msa1071660 | 0.810374 | 1.190028e-50 | -8.615850e-47 |
| Msa0053240 | Msa1083880 | 0.809219 | 2.107958e-50 | -8.615850e-47 |
| Msa0053240 | Msa1093330 | 0.817261 | 3.615085e-52 | -8.615850e-47 |
| Msa0053240 | Msa1131560 | 0.800516 | 1.389488e-48 | -8.615850e-47 |
| Msa0053240 | Msa1158770 | 0.805962 | 1.036123e-49 | -8.615850e-47 |
| Msa0053240 | Msa1166870 | 0.808127 | 3.608116e-50 | -8.615850e-47 |
| Msa0053240 | Msa1176980 | 0.837481 | 5.067359e-57 | -8.615850e-47 |
| Msa0053240 | Msa1179950 | 0.829169 | 5.981869e-55 | -8.615850e-47 |
| Msa0053240 | Msa1179980 | 0.842172 | 3.040674e-58 | -8.615850e-47 |
| Msa0053240 | Msa1184370 | 0.840826 | 6.881469e-58 | -8.615850e-47 |
| Msa0053240 | Msa1192850 | 0.814668 | 1.370269e-51 | -8.615850e-47 |
| Msa0053240 | Msa1201830 | 0.815351 | 9.667018e-52 | -8.615850e-47 |
| Msa0053240 | Msa1208020 | 0.800043 | 1.734049e-48 | -8.615850e-47 |
| Msa0053240 | Msa1226610 | 0.801894 | 7.258726e-49 | -8.615850e-47 |
| Msa0053240 | Msa1227690 | 0.849401 | 3.311195e-60 | -8.615850e-47 |
| Msa0053240 | Msa1256770 | 0.802393 | 5.731167e-49 | -8.615850e-47 |
| Msa0053240 | Msa1270620 | 0.849972 | 2.294417e-60 | -8.615850e-47 |
| Msa0053240 | Msa1270690 | 0.849862 | 2.462182e-60 | -8.615850e-47 |
| Msa0053240 | Msa1278900 | 0.800338 | 1.510184e-48 | -8.615850e-47 |
| Msa0053240 | Msa1294840 | 0.817586 | 3.053749e-52 | -8.615850e-47 |
| Msa0053240 | Msa1299700 | 0.810696 | 1.013566e-50 | -8.615850e-47 |
| Msa0053240 | Msa1306990 | 0.841836 | 3.730606e-58 | -8.615850e-47 |
| Msa0053240 | Msa1327490 | 0.801827 | 7.491871e-49 | -8.615850e-47 |
| Msa0053240 | Msa1331250 | 0.824725 | 6.907266e-54 | -8.615850e-47 |
| Msa0053240 | Msa1343290 | 0.820717 | 5.918019e-53 | -8.615850e-47 |
| Msa0053240 | Msa1345450 | 0.801053 | 1.079346e-48 | -8.615850e-47 |
| Msa0053240 | Msa1363860 | 0.819658 | 1.034713e-52 | -8.615850e-47 |
| Msa0053240 | Msa1369190 | 0.821776 | 3.372300e-53 | -8.615850e-47 |
| Msa0053240 | Msa1372310 | 0.801881 | 7.303273e-49 | -8.615850e-47 |
| Msa0053240 | Msa1389680 | 0.929671 | 4.973197e-93 | -8.615850e-47 |
| Msa0053240 | Msa1397940 | 0.807197 | 5.685517e-50 | -8.615850e-47 |
| Msa0053240 | Msa1432480 | 0.800214 | 1.601060e-48 | -8.615850e-47 |
| Msa0053240 | Msa1435770 | 0.816305 | 5.922071e-52 | -8.615850e-47 |
| Msa0053240 | Msa1438640 | 0.810358 | 1.199661e-50 | -8.615850e-47 |
| Msa0053240 | Msa1442850 | 0.801303 | 9.594268e-49 | -8.615850e-47 |
| Msa0053240 | Msa1459960 | 0.807773 | 4.290331e-50 | -8.615850e-47 |
| Msa0053240 | Msa1462860 | 0.823941 | 1.055850e-53 | -8.615850e-47 |
| Msa0053240 | Msa1465080 | 0.812700 | 3.714938e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0053240 | MtrunA17_Chr1g0151511 | 86.179 | 246 | 22 | 2 | 56 | 289 | 19 | 264 | 2.00e-121 | 350 |
| Msa0053240 | MtrunA17_Chr8g0377581 | 44.167 | 240 | 88 | 7 | 56 | 286 | 18 | 220 | 6.13e-38 | 135 |
| Msa0053240 | MtrunA17_Chr1g0151501 | 87.500 | 32 | 4 | 0 | 294 | 325 | 900 | 931 | 3.84e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0053240 | AT4G33010.2 | 84.848 | 33 | 5 | 0 | 293 | 325 | 882 | 914 | 3.82e-12 | 67.8 |
| Msa0053240 | AT4G33010.1 | 84.848 | 33 | 5 | 0 | 293 | 325 | 882 | 914 | 1.40e-11 | 65.9 |
| Msa0053240 | AT2G26080.1 | 84.848 | 33 | 5 | 0 | 293 | 325 | 888 | 920 | 1.49e-11 | 65.9 |
Find 82 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGACTTAAGAGGCTTTA+AGG | 0.132028 | 1_2:-7672225 | Msa0053240:intron |
| GAAGAGTTTGCCGGCGGTTC+TGG | 0.227820 | 1_2:+7673856 | None:intergenic |
| GGAAAGAAGAAACTAGAATT+TGG | 0.282317 | 1_2:-7673360 | Msa0053240:CDS |
| GTGAAATATCTTATGGATAA+TGG | 0.285026 | 1_2:-7672075 | Msa0053240:CDS |
| AACCGCCGGCAAACTCTTCC+AGG | 0.307525 | 1_2:-7673852 | Msa0053240:intron |
| AAGAAGGAGAAGAGTGTAGA+AGG | 0.312453 | 1_2:-7673246 | Msa0053240:CDS |
| CCGCCGGACTTGGTAGTTAC+AGG | 0.340301 | 1_2:-7673893 | Msa0053240:CDS |
| AGATATTTCACAACATCTTC+AGG | 0.369345 | 1_2:+7672087 | None:intergenic |
| AAGAAGAATGGAAGTGAGTT+AGG | 0.374907 | 1_2:-7673327 | Msa0053240:CDS |
| TGAAAAGGAGAAGAATTTAG+AGG | 0.379641 | 1_2:-7673139 | Msa0053240:intron |
| GAATTCATTGTTGACTTAAG+AGG | 0.383562 | 1_2:-7672233 | Msa0053240:CDS |
| GCAGTCGCACCGAGCTCAAT+TGG | 0.383904 | 1_2:-7673926 | Msa0053240:CDS |
| GTTGTCAGCAGAGACAAATT+TGG | 0.400587 | 1_2:+7673617 | None:intergenic |
| AGATGTTGTGAAATATCTTA+TGG | 0.408852 | 1_2:-7672082 | Msa0053240:CDS |
| ATTCTCGGGATGTAGAAATT+AGG | 0.415570 | 1_2:-7673029 | Msa0053240:CDS |
| CCGGAAAACGCCGCCGGACT+TGG | 0.419030 | 1_2:-7673903 | Msa0053240:CDS |
| AGAAGGAGAAGAGTGTAGAA+GGG | 0.423276 | 1_2:-7673245 | Msa0053240:CDS |
| TTTGCCGGCGGTTCTGGCGG+TGG | 0.425742 | 1_2:+7673862 | None:intergenic |
| GAGCTCGGTGCGACTGCAGC+TGG | 0.432099 | 1_2:+7673932 | None:intergenic |
| GAAGACACGGAAGAGAGATC+TGG | 0.440783 | 1_2:-7672922 | Msa0053240:CDS |
| GTGGAGGGCTAGTTGATTCT+CGG | 0.450550 | 1_2:-7673044 | Msa0053240:CDS |
| TTAAACCGGCGCAGTCTCAT+AGG | 0.455996 | 1_2:-7673515 | Msa0053240:CDS |
| TGAGCTTAATCAACTTCATA+AGG | 0.457180 | 1_2:-7673541 | Msa0053240:CDS |
| TTTCCAATGTAGAATACTGC+TGG | 0.457929 | 1_2:-7672117 | Msa0053240:intron |
| ATGTGAAGGTTGAAAGTAAA+GGG | 0.462456 | 1_2:-7673218 | Msa0053240:CDS |
| GGAGAGAGTGTAAAGAAGAA+TGG | 0.469789 | 1_2:-7673339 | Msa0053240:CDS |
| CTCCTCAGGAGTTAGAAATT+AGG | 0.475036 | 1_2:-7673188 | Msa0053240:CDS |
| AAATGAAGTGGGGAGTGAAA+AGG | 0.480460 | 1_2:-7673154 | Msa0053240:CDS |
| AACAGATGATAGTGGTAGAT+TGG | 0.498404 | 1_2:-7673472 | Msa0053240:CDS |
| TGGAGGGCTAGTTGATTCTC+GGG | 0.502825 | 1_2:-7673043 | Msa0053240:CDS |
| AAGACACGGAAGAGAGATCT+GGG | 0.505868 | 1_2:-7672921 | Msa0053240:CDS |
| ACTTCATAAGGAGCTTAAAC+CGG | 0.507615 | 1_2:-7673529 | Msa0053240:CDS |
| GAATGGAAGTGAGTTAGGTC+AGG | 0.510402 | 1_2:-7673322 | Msa0053240:CDS |
| GAAAACCTGGAAGAGTTTGC+CGG | 0.511563 | 1_2:+7673847 | None:intergenic |
| GAAGTTGTTGGTTCTGTCGT+GGG | 0.512214 | 1_2:-7673411 | Msa0053240:CDS |
| GAAGGAGAAGAGTGTAGAAG+GGG | 0.517307 | 1_2:-7673244 | Msa0053240:CDS |
| CTCCCTGTAACTACCAAGTC+CGG | 0.517943 | 1_2:+7673890 | None:intergenic |
| CGAGAAGAAGAACAAGAAGA+AGG | 0.521154 | 1_2:-7673262 | Msa0053240:CDS |
| AATTCCACCGCCAGAACCGC+CGG | 0.521284 | 1_2:-7673866 | Msa0053240:CDS |
| TAAAGGGATAGTTCCTCCTC+AGG | 0.521560 | 1_2:-7673202 | Msa0053240:CDS |
| GAGTTTGCCGGCGGTTCTGG+CGG | 0.531512 | 1_2:+7673859 | None:intergenic |
| AATTGGCCGGAAAACGCCGC+CGG | 0.533188 | 1_2:-7673909 | Msa0053240:CDS |
| TGAAGTTGTTGGTTCTGTCG+TGG | 0.542751 | 1_2:-7673412 | Msa0053240:CDS |
| AATGTGAAGGTTGAAAGTAA+AGG | 0.544852 | 1_2:-7673219 | Msa0053240:CDS |
| AGACGGAGCAAAGGAAGAAG+AGG | 0.546821 | 1_2:-7672951 | Msa0053240:CDS |
| CATTGAAAGCCGTGGATGCA+CGG | 0.548708 | 1_2:+7673562 | None:intergenic |
| AAGAGGGAAAGACGGAGCAA+AGG | 0.550638 | 1_2:-7672960 | Msa0053240:CDS |
| AAACCTGGTCGTGAAGTTGT+TGG | 0.551304 | 1_2:-7673423 | Msa0053240:CDS |
| TCGCACCGAGCTCAATTGGC+CGG | 0.564441 | 1_2:-7673922 | Msa0053240:CDS |
| GAGTTAGGTCAGGACGATGA+AGG | 0.567638 | 1_2:-7673312 | Msa0053240:CDS |
| GAGTAAATTACATGCTGAAG+AGG | 0.574245 | 1_2:-7672977 | Msa0053240:CDS |
| AATGGTGTGAAGAGTGAAAG+TGG | 0.576054 | 1_2:-7673063 | Msa0053240:CDS |
| GGGACGAGAACAACAGAAAG+AGG | 0.576625 | 1_2:-7673103 | Msa0053240:CDS |
| TTCCAATGTAGAATACTGCT+GGG | 0.577015 | 1_2:-7672116 | Msa0053240:intron |
| TGTAGAAGGGGAGAATGTGA+AGG | 0.579317 | 1_2:-7673232 | Msa0053240:CDS |
| GATTGGTCGAAAGTACTGTT+CGG | 0.581744 | 1_2:-7673455 | Msa0053240:CDS |
| TACCACTATCATCTGTTGCA+TGG | 0.584555 | 1_2:+7673478 | None:intergenic |
| AGCCATGCAACAGATGATAG+TGG | 0.587057 | 1_2:-7673480 | Msa0053240:CDS |
| ATTAAGCTCATTGAAAGCCG+TGG | 0.591302 | 1_2:+7673554 | None:intergenic |
| GACGATGAAGGAAGAACGGA+GGG | 0.595325 | 1_2:-7673300 | Msa0053240:CDS |
| TTATCTGCTCCGTGCATCCA+CGG | 0.595538 | 1_2:-7673571 | Msa0053240:CDS |
| AGTAAATTACATGCTGAAGA+GGG | 0.598011 | 1_2:-7672976 | Msa0053240:CDS |
| CTGGTTTCTGATGCAAACGA+GGG | 0.598123 | 1_2:+7673951 | None:intergenic |
| GACCTAATTTCTAACTCCTG+AGG | 0.598620 | 1_2:+7673186 | None:intergenic |
| CGCCGGACTTGGTAGTTACA+GGG | 0.601379 | 1_2:-7673892 | Msa0053240:CDS |
| GAACCAACAACTTCACGACC+AGG | 0.602335 | 1_2:+7673420 | None:intergenic |
| GCTGGTTTCTGATGCAAACG+AGG | 0.604991 | 1_2:+7673950 | None:intergenic |
| GTTCGGAGTGATGTTAAACC+TGG | 0.607851 | 1_2:-7673438 | Msa0053240:CDS |
| GTTGTTGGTTCTGTCGTGGG+TGG | 0.608189 | 1_2:-7673408 | Msa0053240:CDS |
| ACGAGAACAACAGAAAGAGG+TGG | 0.612657 | 1_2:-7673100 | Msa0053240:CDS |
| TCAGGACGATGAAGGAAGAA+CGG | 0.621358 | 1_2:-7673304 | Msa0053240:CDS |
| ACATGCTGAAGAGGGAAAGA+CGG | 0.635975 | 1_2:-7672968 | Msa0053240:CDS |
| TTCTTCCTATGAGACTGCGC+CGG | 0.636496 | 1_2:+7673510 | None:intergenic |
| GAAGAGGAAGAATGAAGACA+CGG | 0.643138 | 1_2:-7672935 | Msa0053240:CDS |
| AAATTTGTCTCTGCTGACAA+CGG | 0.644626 | 1_2:-7673615 | Msa0053240:CDS |
| CTAATTTCTAACTCCTGAGG+AGG | 0.647828 | 1_2:+7673189 | None:intergenic |
| GTGTGAAGAGTGAAAGTGGA+GGG | 0.650346 | 1_2:-7673059 | Msa0053240:CDS |
| GGACGATGAAGGAAGAACGG+AGG | 0.651421 | 1_2:-7673301 | Msa0053240:CDS |
| AACCTGGAAGAGTTTGCCGG+CGG | 0.693894 | 1_2:+7673850 | None:intergenic |
| AGACACGGAAGAGAGATCTG+GGG | 0.742079 | 1_2:-7672920 | Msa0053240:CDS |
| CCTGTAACTACCAAGTCCGG+CGG | 0.742344 | 1_2:+7673893 | None:intergenic |
| GGTGTGAAGAGTGAAAGTGG+AGG | 0.804724 | 1_2:-7673060 | Msa0053240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAAAATGAAAACGATTA+CGG | - | chr1_2:7672367-7672386 | Msa0053240:intron | 20.0% |
| !!! | AATCTTTTTCAATTTTGATC+AGG | - | chr1_2:7673291-7673310 | Msa0053240:CDS | 20.0% |
| !! | TGACAAAAATAATGTAATAG+AGG | + | chr1_2:7673399-7673418 | None:intergenic | 20.0% |
| !!! | TACATTATTTTTGTCATACT+AGG | - | chr1_2:7673403-7673422 | Msa0053240:intron | 20.0% |
| !!! | ACATTATTTTTGTCATACTA+GGG | - | chr1_2:7673404-7673423 | Msa0053240:intron | 20.0% |
| !! | CATTCACAAAAACAAAATAT+AGG | + | chr1_2:7673587-7673606 | None:intergenic | 20.0% |
| !!! | CATTTTTTCTTATGCTTTCA+CGG | + | chr1_2:7672357-7672376 | None:intergenic | 25.0% |
| ! | GAAAAAATGAAAACGATTAC+GGG | - | chr1_2:7672368-7672387 | Msa0053240:intron | 25.0% |
| ! | AGTCTAAGAAGAAAATGAAA+AGG | - | chr1_2:7673167-7673186 | Msa0053240:CDS | 25.0% |
| ! | AAACAACTTCAATCATCAAA+TGG | + | chr1_2:7673867-7673886 | None:intergenic | 25.0% |
| ! | AGATGTTGTGAAATATCTTA+TGG | - | chr1_2:7673979-7673998 | Msa0053240:CDS | 25.0% |
| ! | GTGAAATATCTTATGGATAA+TGG | - | chr1_2:7673986-7674005 | Msa0053240:CDS | 25.0% |
| GAAATGAGAAAAGGAGAAAA+AGG | + | chr1_2:7672239-7672258 | None:intergenic | 30.0% | |
| TGGATATAGGAAATGAGAAA+AGG | + | chr1_2:7672248-7672267 | None:intergenic | 30.0% | |
| AAACGCGATTATATGGATAT+AGG | + | chr1_2:7672261-7672280 | None:intergenic | 30.0% | |
| !!! | TTTTCTGAAACGCGATTATA+TGG | + | chr1_2:7672268-7672287 | None:intergenic | 30.0% |
| AGCTATATATGTATGTGTGA+AGG | - | chr1_2:7672326-7672345 | Msa0053240:intron | 30.0% | |
| TGAGCTTAATCAACTTCATA+AGG | - | chr1_2:7672520-7672539 | Msa0053240:intron | 30.0% | |
| GGAAAGAAGAAACTAGAATT+TGG | - | chr1_2:7672701-7672720 | Msa0053240:intron | 30.0% | |
| AATGTGAAGGTTGAAAGTAA+AGG | - | chr1_2:7672842-7672861 | Msa0053240:intron | 30.0% | |
| ATGTGAAGGTTGAAAGTAAA+GGG | - | chr1_2:7672843-7672862 | Msa0053240:intron | 30.0% | |
| TCGAAAAAGAGAAATGAAGT+GGG | - | chr1_2:7672896-7672915 | Msa0053240:intron | 30.0% | |
| TGAAAAGGAGAAGAATTTAG+AGG | - | chr1_2:7672922-7672941 | Msa0053240:CDS | 30.0% | |
| AGTAAATTACATGCTGAAGA+GGG | - | chr1_2:7673085-7673104 | Msa0053240:CDS | 30.0% | |
| AAAATGAAAAGGAAACACGA+GGG | - | chr1_2:7673178-7673197 | Msa0053240:CDS | 30.0% | |
| GTACTATCATAGAACATATG+AGG | - | chr1_2:7673357-7673376 | Msa0053240:CDS | 30.0% | |
| ! | TGATAGCTGTTAAATCGTTT+TGG | - | chr1_2:7673462-7673481 | Msa0053240:CDS | 30.0% |
| ! | ATGACAGATTTTCACTTGTA+AGG | - | chr1_2:7673491-7673510 | Msa0053240:CDS | 30.0% |
| TAAAGTATGAGTGAAAACCA+AGG | + | chr1_2:7673745-7673764 | None:intergenic | 30.0% | |
| !! | GAATTCATTGTTGACTTAAG+AGG | - | chr1_2:7673828-7673847 | Msa0053240:intron | 30.0% |
| AGATATTTCACAACATCTTC+AGG | + | chr1_2:7673977-7673996 | None:intergenic | 30.0% | |
| AAAGGAGAAAAAGGAAAACC+TGG | + | chr1_2:7672230-7672249 | None:intergenic | 35.0% | |
| AAATTTGTCTCTGCTGACAA+CGG | - | chr1_2:7672446-7672465 | Msa0053240:intron | 35.0% | |
| ACTTCATAAGGAGCTTAAAC+CGG | - | chr1_2:7672532-7672551 | Msa0053240:intron | 35.0% | |
| !! | AACAGATGATAGTGGTAGAT+TGG | - | chr1_2:7672589-7672608 | Msa0053240:intron | 35.0% |
| AAGAAGAATGGAAGTGAGTT+AGG | - | chr1_2:7672734-7672753 | Msa0053240:intron | 35.0% | |
| GTCGAAAAAGAGAAATGAAG+TGG | - | chr1_2:7672895-7672914 | Msa0053240:intron | 35.0% | |
| CGAAAAAGAGAAATGAAGTG+GGG | - | chr1_2:7672897-7672916 | Msa0053240:intron | 35.0% | |
| AAGGAGAAGAATTTAGAGGT+TGG | - | chr1_2:7672926-7672945 | Msa0053240:CDS | 35.0% | |
| TTTAGAGGTTGGAAATGTGA+AGG | - | chr1_2:7672937-7672956 | Msa0053240:CDS | 35.0% | |
| TTAGAGGTTGGAAATGTGAA+GGG | - | chr1_2:7672938-7672957 | Msa0053240:CDS | 35.0% | |
| GTGGAGAAAAAACTAAGCAA+TGG | - | chr1_2:7672980-7672999 | Msa0053240:CDS | 35.0% | |
| ATTCTCGGGATGTAGAAATT+AGG | - | chr1_2:7673032-7673051 | Msa0053240:CDS | 35.0% | |
| ! | TCGAAAAAGAAGCATGAAAC+TGG | - | chr1_2:7673055-7673074 | Msa0053240:CDS | 35.0% |
| ! | CGAAAAAGAAGCATGAAACT+GGG | - | chr1_2:7673056-7673075 | Msa0053240:CDS | 35.0% |
| GAGTAAATTACATGCTGAAG+AGG | - | chr1_2:7673084-7673103 | Msa0053240:CDS | 35.0% | |
| GAAAATGAAAAGGAAACACG+AGG | - | chr1_2:7673177-7673196 | Msa0053240:CDS | 35.0% | |
| CGAGGGTTAAGCTTAAATTT+AGG | - | chr1_2:7673195-7673214 | Msa0053240:CDS | 35.0% | |
| ! | ATCAGGAATTTTCACTTGCA+AGG | - | chr1_2:7673308-7673327 | Msa0053240:CDS | 35.0% |
| CATATGAGGAATTCGCTTAT+TGG | - | chr1_2:7673371-7673390 | Msa0053240:intron | 35.0% | |
| TGAGATGCAGTGTTAAGTTA+CGG | - | chr1_2:7673638-7673657 | Msa0053240:intron | 35.0% | |
| GTTATTTATGACTTCCTCCT+CGG | + | chr1_2:7673670-7673689 | None:intergenic | 35.0% | |
| GGTTCATCATATATGACACT+GGG | + | chr1_2:7673724-7673743 | None:intergenic | 35.0% | |
| AGGTTCATCATATATGACAC+TGG | + | chr1_2:7673725-7673744 | None:intergenic | 35.0% | |
| GTGTCATATATGATGAACCT+TGG | - | chr1_2:7673725-7673744 | Msa0053240:intron | 35.0% | |
| !! | TGTTGACTTAAGAGGCTTTA+AGG | - | chr1_2:7673836-7673855 | Msa0053240:intron | 35.0% |
| TTTCCAATGTAGAATACTGC+TGG | - | chr1_2:7673944-7673963 | Msa0053240:CDS | 35.0% | |
| TTCCAATGTAGAATACTGCT+GGG | - | chr1_2:7673945-7673964 | Msa0053240:CDS | 35.0% | |
| GTTGTCAGCAGAGACAAATT+TGG | + | chr1_2:7672447-7672466 | None:intergenic | 40.0% | |
| ATTAAGCTCATTGAAAGCCG+TGG | + | chr1_2:7672510-7672529 | None:intergenic | 40.0% | |
| TACCACTATCATCTGTTGCA+TGG | + | chr1_2:7672586-7672605 | None:intergenic | 40.0% | |
| !! | GATTGGTCGAAAGTACTGTT+CGG | - | chr1_2:7672606-7672625 | Msa0053240:intron | 40.0% |
| ! | GAGAAGCGTGATAAGAAAGA+TGG | - | chr1_2:7672680-7672699 | Msa0053240:intron | 40.0% |
| ! | GGAGAGAGTGTAAAGAAGAA+TGG | - | chr1_2:7672722-7672741 | Msa0053240:intron | 40.0% |
| CGAGAAGAAGAACAAGAAGA+AGG | - | chr1_2:7672799-7672818 | Msa0053240:intron | 40.0% | |
| ! | AAGAAGGAGAAGAGTGTAGA+AGG | - | chr1_2:7672815-7672834 | Msa0053240:intron | 40.0% |
| ! | AGAAGGAGAAGAGTGTAGAA+GGG | - | chr1_2:7672816-7672835 | Msa0053240:intron | 40.0% |
| CTAATTTCTAACTCCTGAGG+AGG | + | chr1_2:7672875-7672894 | None:intergenic | 40.0% | |
| CTCCTCAGGAGTTAGAAATT+AGG | - | chr1_2:7672873-7672892 | Msa0053240:intron | 40.0% | |
| GACCTAATTTCTAACTCCTG+AGG | + | chr1_2:7672878-7672897 | None:intergenic | 40.0% | |
| AAATGAAGTGGGGAGTGAAA+AGG | - | chr1_2:7672907-7672926 | Msa0053240:intron | 40.0% | |
| AATGGTGTGAAGAGTGAAAG+TGG | - | chr1_2:7672998-7673017 | Msa0053240:CDS | 40.0% | |
| GAAGAGGAAGAATGAAGACA+CGG | - | chr1_2:7673126-7673145 | Msa0053240:intron | 40.0% | |
| TTGCAAGGTTGCTTACATTG+TGG | - | chr1_2:7673323-7673342 | Msa0053240:CDS | 40.0% | |
| TTGTAAGGTTGCTTGCAATG+TGG | - | chr1_2:7673506-7673525 | Msa0053240:CDS | 40.0% | |
| TGTGGCATCACTTTATGTAG+TGG | - | chr1_2:7673524-7673543 | Msa0053240:CDS | 40.0% | |
| GTAGTGGCATATAACATCAG+AGG | - | chr1_2:7673540-7673559 | Msa0053240:CDS | 40.0% | |
| ! | TTAAGTTACGGATTTTGCCG+AGG | - | chr1_2:7673650-7673669 | Msa0053240:intron | 40.0% |
| CTGGGAACACGGAAAAAAAT+TGG | + | chr1_2:7673706-7673725 | None:intergenic | 40.0% | |
| CATATATGACACTGGGAACA+CGG | + | chr1_2:7673717-7673736 | None:intergenic | 40.0% | |
| ! | TATTCCCCAATTCTTTTCCG+CGG | - | chr1_2:7673783-7673802 | Msa0053240:intron | 40.0% |
| ! | CAACTGTTTAATTGACTCCG+CGG | + | chr1_2:7673803-7673822 | None:intergenic | 40.0% |
| ATCCCAGCAGTATTCTACAT+TGG | + | chr1_2:7673950-7673969 | None:intergenic | 40.0% | |
| CTGGTTTCTGATGCAAACGA+GGG | + | chr1_2:7672113-7672132 | None:intergenic | 45.0% | |
| GAAAACCTGGAAGAGTTTGC+CGG | + | chr1_2:7672217-7672236 | None:intergenic | 45.0% | |
| AGCCATGCAACAGATGATAG+TGG | - | chr1_2:7672581-7672600 | Msa0053240:intron | 45.0% | |
| GTTCGGAGTGATGTTAAACC+TGG | - | chr1_2:7672623-7672642 | Msa0053240:intron | 45.0% | |
| AAACCTGGTCGTGAAGTTGT+TGG | - | chr1_2:7672638-7672657 | Msa0053240:intron | 45.0% | |
| !! | TGAAGTTGTTGGTTCTGTCG+TGG | - | chr1_2:7672649-7672668 | Msa0053240:intron | 45.0% |
| !! | GAAGTTGTTGGTTCTGTCGT+GGG | - | chr1_2:7672650-7672669 | Msa0053240:intron | 45.0% |
| GAATGGAAGTGAGTTAGGTC+AGG | - | chr1_2:7672739-7672758 | Msa0053240:intron | 45.0% | |
| TCAGGACGATGAAGGAAGAA+CGG | - | chr1_2:7672757-7672776 | Msa0053240:intron | 45.0% | |
| ! | GAAGGAGAAGAGTGTAGAAG+GGG | - | chr1_2:7672817-7672836 | Msa0053240:intron | 45.0% |
| TGTAGAAGGGGAGAATGTGA+AGG | - | chr1_2:7672829-7672848 | Msa0053240:intron | 45.0% | |
| TAAAGGGATAGTTCCTCCTC+AGG | - | chr1_2:7672859-7672878 | Msa0053240:intron | 45.0% | |
| ACGAGAACAACAGAAAGAGG+TGG | - | chr1_2:7672961-7672980 | Msa0053240:CDS | 45.0% | |
| GTGTGAAGAGTGAAAGTGGA+GGG | - | chr1_2:7673002-7673021 | Msa0053240:CDS | 45.0% | |
| ACATGCTGAAGAGGGAAAGA+CGG | - | chr1_2:7673093-7673112 | Msa0053240:CDS | 45.0% | |
| AAGACACGGAAGAGAGATCT+GGG | - | chr1_2:7673140-7673159 | Msa0053240:CDS | 45.0% | |
| TGATAGCCAACTCCACTTAG+TGG | - | chr1_2:7673235-7673254 | Msa0053240:CDS | 45.0% | |
| GATAGCCAACTCCACTTAGT+GGG | - | chr1_2:7673236-7673255 | Msa0053240:CDS | 45.0% | |
| CTTATCCCACTAAGTGGAGT+TGG | + | chr1_2:7673244-7673263 | None:intergenic | 45.0% | |
| CAACTCCACTTAGTGGGATA+AGG | - | chr1_2:7673242-7673261 | Msa0053240:CDS | 45.0% | |
| TGACTCCGCGGAAAAGAATT+GGG | + | chr1_2:7673791-7673810 | None:intergenic | 45.0% | |
| TTGACTCCGCGGAAAAGAAT+TGG | + | chr1_2:7673792-7673811 | None:intergenic | 45.0% | |
| ! | GCTGGTTTCTGATGCAAACG+AGG | + | chr1_2:7672114-7672133 | None:intergenic | 50.0% |
| ! | GTTTTCCGGCCAATTGAGCT+CGG | + | chr1_2:7672147-7672166 | None:intergenic | 50.0% |
| CTCCCTGTAACTACCAAGTC+CGG | + | chr1_2:7672174-7672193 | None:intergenic | 50.0% | |
| TTATCTGCTCCGTGCATCCA+CGG | - | chr1_2:7672490-7672509 | Msa0053240:intron | 50.0% | |
| CATTGAAAGCCGTGGATGCA+CGG | + | chr1_2:7672502-7672521 | None:intergenic | 50.0% | |
| TTAAACCGGCGCAGTCTCAT+AGG | - | chr1_2:7672546-7672565 | Msa0053240:intron | 50.0% | |
| TTCTTCCTATGAGACTGCGC+CGG | + | chr1_2:7672554-7672573 | None:intergenic | 50.0% | |
| GAACCAACAACTTCACGACC+AGG | + | chr1_2:7672644-7672663 | None:intergenic | 50.0% | |
| GAGTTAGGTCAGGACGATGA+AGG | - | chr1_2:7672749-7672768 | Msa0053240:intron | 50.0% | |
| GACGATGAAGGAAGAACGGA+GGG | - | chr1_2:7672761-7672780 | Msa0053240:intron | 50.0% | |
| GGGACGAGAACAACAGAAAG+AGG | - | chr1_2:7672958-7672977 | Msa0053240:CDS | 50.0% | |
| GGTGTGAAGAGTGAAAGTGG+AGG | - | chr1_2:7673001-7673020 | Msa0053240:CDS | 50.0% | |
| !! | GTGGAGGGCTAGTTGATTCT+CGG | - | chr1_2:7673017-7673036 | Msa0053240:CDS | 50.0% |
| !! | TGGAGGGCTAGTTGATTCTC+GGG | - | chr1_2:7673018-7673037 | Msa0053240:CDS | 50.0% |
| AAGAGGGAAAGACGGAGCAA+AGG | - | chr1_2:7673101-7673120 | Msa0053240:CDS | 50.0% | |
| AGACGGAGCAAAGGAAGAAG+AGG | - | chr1_2:7673110-7673129 | Msa0053240:intron | 50.0% | |
| GAAGACACGGAAGAGAGATC+TGG | - | chr1_2:7673139-7673158 | Msa0053240:intron | 50.0% | |
| AGACACGGAAGAGAGATCTG+GGG | - | chr1_2:7673141-7673160 | Msa0053240:CDS | 50.0% | |
| ! | CGAAGCCTTATCCCACTAAG+TGG | + | chr1_2:7673250-7673269 | None:intergenic | 50.0% |
| ! | AGTTACGGATTTTGCCGAGG+AGG | - | chr1_2:7673653-7673672 | Msa0053240:intron | 50.0% |
| GACTCCGCGGAAAAGAATTG+GGG | + | chr1_2:7673790-7673809 | None:intergenic | 50.0% | |
| CCTGTAACTACCAAGTCCGG+CGG | + | chr1_2:7672171-7672190 | None:intergenic | 55.0% | |
| ! | CGCCGGACTTGGTAGTTACA+GGG | - | chr1_2:7672169-7672188 | Msa0053240:intron | 55.0% |
| AACCTGGAAGAGTTTGCCGG+CGG | + | chr1_2:7672214-7672233 | None:intergenic | 55.0% | |
| !! | GTTGTTGGTTCTGTCGTGGG+TGG | - | chr1_2:7672653-7672672 | Msa0053240:intron | 55.0% |
| GGACGATGAAGGAAGAACGG+AGG | - | chr1_2:7672760-7672779 | Msa0053240:intron | 55.0% | |
| GCAGTCGCACCGAGCTCAAT+TGG | - | chr1_2:7672135-7672154 | Msa0053240:intron | 60.0% | |
| TCGCACCGAGCTCAATTGGC+CGG | - | chr1_2:7672139-7672158 | Msa0053240:intron | 60.0% | |
| AATTGGCCGGAAAACGCCGC+CGG | - | chr1_2:7672152-7672171 | Msa0053240:intron | 60.0% | |
| ! | CCGCCGGACTTGGTAGTTAC+AGG | - | chr1_2:7672168-7672187 | Msa0053240:intron | 60.0% |
| AATTCCACCGCCAGAACCGC+CGG | - | chr1_2:7672195-7672214 | Msa0053240:intron | 60.0% | |
| GAAGAGTTTGCCGGCGGTTC+TGG | + | chr1_2:7672208-7672227 | None:intergenic | 60.0% | |
| AACCGCCGGCAAACTCTTCC+AGG | - | chr1_2:7672209-7672228 | Msa0053240:intron | 60.0% | |
| ! | CCAAGTCCGGCGGCGTTTTC+CGG | + | chr1_2:7672161-7672180 | None:intergenic | 65.0% |
| ! | GAGTTTGCCGGCGGTTCTGG+CGG | + | chr1_2:7672205-7672224 | None:intergenic | 65.0% |
| !! | GAGCTCGGTGCGACTGCAGC+TGG | + | chr1_2:7672132-7672151 | None:intergenic | 70.0% |
| CCGGAAAACGCCGCCGGACT+TGG | - | chr1_2:7672158-7672177 | Msa0053240:intron | 70.0% | |
| ! | TTTGCCGGCGGTTCTGGCGG+TGG | + | chr1_2:7672202-7672221 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 7672065 | 7674018 | 7672065 | ID=Msa0053240;Name=Msa0053240 |
| chr1_2 | mRNA | 7672065 | 7674018 | 7672065 | ID=Msa0053240-mRNA-1;Parent=Msa0053240;Name=Msa0053240-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|0|0|7|0|325 |
| chr1_2 | exon | 7673853 | 7674018 | 7673853 | ID=Msa0053240-mRNA-1:exon:2673;Parent=Msa0053240-mRNA-1 |
| chr1_2 | exon | 7673406 | 7673654 | 7673406 | ID=Msa0053240-mRNA-1:exon:2672;Parent=Msa0053240-mRNA-1 |
| chr1_2 | exon | 7673140 | 7673384 | 7673140 | ID=Msa0053240-mRNA-1:exon:2671;Parent=Msa0053240-mRNA-1 |
| chr1_2 | exon | 7672916 | 7673124 | 7672916 | ID=Msa0053240-mRNA-1:exon:2670;Parent=Msa0053240-mRNA-1 |
| chr1_2 | exon | 7672303 | 7672310 | 7672303 | ID=Msa0053240-mRNA-1:exon:2669;Parent=Msa0053240-mRNA-1 |
| chr1_2 | exon | 7672226 | 7672263 | 7672226 | ID=Msa0053240-mRNA-1:exon:2668;Parent=Msa0053240-mRNA-1 |
| chr1_2 | exon | 7672065 | 7672127 | 7672065 | ID=Msa0053240-mRNA-1:exon:2667;Parent=Msa0053240-mRNA-1 |
| chr1_2 | CDS | 7673853 | 7674018 | 7673853 | ID=Msa0053240-mRNA-1:cds;Parent=Msa0053240-mRNA-1 |
| chr1_2 | CDS | 7673406 | 7673654 | 7673406 | ID=Msa0053240-mRNA-1:cds;Parent=Msa0053240-mRNA-1 |
| chr1_2 | CDS | 7673140 | 7673384 | 7673140 | ID=Msa0053240-mRNA-1:cds;Parent=Msa0053240-mRNA-1 |
| chr1_2 | CDS | 7672916 | 7673124 | 7672916 | ID=Msa0053240-mRNA-1:cds;Parent=Msa0053240-mRNA-1 |
| chr1_2 | CDS | 7672303 | 7672310 | 7672303 | ID=Msa0053240-mRNA-1:cds;Parent=Msa0053240-mRNA-1 |
| chr1_2 | CDS | 7672226 | 7672263 | 7672226 | ID=Msa0053240-mRNA-1:cds;Parent=Msa0053240-mRNA-1 |
| chr1_2 | CDS | 7672065 | 7672127 | 7672065 | ID=Msa0053240-mRNA-1:cds;Parent=Msa0053240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
