Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0624040 | XP_003605778.1 | 93.162 | 117 | 7 | 1 | 3 | 118 | 2 | 118 | 1.28e-75 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004340 | Msa0624040 | 0.811470 | 6.888273e-51 | -8.615850e-47 |
| Msa0012380 | Msa0624040 | 0.819603 | 1.065136e-52 | -8.615850e-47 |
| Msa0014410 | Msa0624040 | 0.801493 | 8.774199e-49 | -8.615850e-47 |
| Msa0044180 | Msa0624040 | 0.824433 | 8.093932e-54 | -8.615850e-47 |
| Msa0064550 | Msa0624040 | 0.852326 | 4.968007e-61 | -8.615850e-47 |
| Msa0109490 | Msa0624040 | 0.819101 | 1.386080e-52 | -8.615850e-47 |
| Msa0604880 | Msa0624040 | 0.812546 | 4.015114e-51 | -8.615850e-47 |
| Msa0605250 | Msa0624040 | 0.847421 | 1.169054e-59 | -8.615850e-47 |
| Msa0609790 | Msa0624040 | 0.807118 | 5.908287e-50 | -8.615850e-47 |
| Msa0610810 | Msa0624040 | 0.812035 | 5.191629e-51 | -8.615850e-47 |
| Msa0622250 | Msa0624040 | 0.810393 | 1.178585e-50 | -8.615850e-47 |
| Msa0624040 | Msa0627050 | 0.806183 | 9.307222e-50 | -8.615850e-47 |
| Msa0624040 | Msa0636720 | 0.805294 | 1.430760e-49 | -8.615850e-47 |
| Msa0624040 | Msa0646190 | 0.850117 | 2.089770e-60 | -8.615850e-47 |
| Msa0624040 | Msa0649310 | 0.819693 | 1.015926e-52 | -8.615850e-47 |
| Msa0624040 | Msa0651310 | 0.846626 | 1.930234e-59 | -8.615850e-47 |
| Msa0624040 | Msa0675010 | 0.811606 | 6.436477e-51 | -8.615850e-47 |
| Msa0624040 | Msa0676100 | 0.845820 | 3.199859e-59 | -8.615850e-47 |
| Msa0624040 | Msa0679780 | 0.801495 | 8.764607e-49 | -8.615850e-47 |
| Msa0624040 | Msa0681210 | 0.846087 | 2.706146e-59 | -8.615850e-47 |
| Msa0624040 | Msa0685890 | 0.807228 | 5.601153e-50 | -8.615850e-47 |
| Msa0624040 | Msa0693400 | 0.819449 | 1.154450e-52 | -8.615850e-47 |
| Msa0624040 | Msa0693500 | 0.816742 | 4.728298e-52 | -8.615850e-47 |
| Msa0624040 | Msa0702200 | 0.800320 | 1.523419e-48 | -8.615850e-47 |
| Msa0624040 | Msa0705810 | 0.831727 | 1.417100e-55 | -8.615850e-47 |
| Msa0624040 | Msa0748330 | 0.804542 | 2.054962e-49 | -8.615850e-47 |
| Msa0624040 | Msa0790640 | 0.836205 | 1.072539e-56 | -8.615850e-47 |
| Msa0624040 | Msa0879650 | 0.854109 | 1.532156e-61 | -8.615850e-47 |
| Msa0624040 | Msa0887100 | 0.814357 | 1.605630e-51 | -8.615850e-47 |
| Msa0624040 | Msa0892020 | 0.822505 | 2.285008e-53 | -8.615850e-47 |
| Msa0624040 | Msa0899730 | 0.811208 | 7.853752e-51 | -8.615850e-47 |
| Msa0624040 | Msa0928940 | 0.840813 | 6.934977e-58 | -8.615850e-47 |
| Msa0624040 | Msa0928970 | 0.812287 | 4.574803e-51 | -8.615850e-47 |
| Msa0624040 | Msa0956910 | 0.814241 | 1.702904e-51 | -8.615850e-47 |
| Msa0624040 | Msa0964650 | 0.812243 | 4.676718e-51 | -8.615850e-47 |
| Msa0624040 | Msa0984000 | 0.842299 | 2.814577e-58 | -8.615850e-47 |
| Msa0624040 | Msa1082150 | 0.829298 | 5.565304e-55 | -8.615850e-47 |
| Msa0624040 | Msa1122720 | 0.820817 | 5.613850e-53 | -8.615850e-47 |
| Msa0624040 | Msa1133440 | 0.801945 | 7.087543e-49 | -8.615850e-47 |
| Msa0624040 | Msa1144730 | 0.848296 | 6.708545e-60 | -8.615850e-47 |
| Msa0624040 | Msa1173380 | 0.844904 | 5.663854e-59 | -8.615850e-47 |
| Msa0624040 | Msa1182800 | 0.800438 | 1.441207e-48 | -8.615850e-47 |
| Msa0624040 | Msa1219060 | 0.801679 | 8.034044e-49 | -8.615850e-47 |
| Msa0624040 | Msa1236860 | 0.806736 | 7.116313e-50 | -8.615850e-47 |
| Msa0624040 | Msa1255020 | 0.842103 | 3.172206e-58 | -8.615850e-47 |
| Msa0624040 | Msa1295470 | 0.839348 | 1.671706e-57 | -8.615850e-47 |
| Msa0624040 | Msa1338590 | 0.833803 | 4.322865e-56 | -8.615850e-47 |
| Msa0624040 | Msa1349810 | 0.865273 | 6.656019e-65 | -8.615850e-47 |
| Msa0624040 | Msa1358340 | 0.804723 | 1.883940e-49 | -8.615850e-47 |
| Msa0624040 | Msa1359890 | 0.841108 | 5.804156e-58 | -8.615850e-47 |
| Msa0624040 | Msa1369180 | 0.813861 | 2.065747e-51 | -8.615850e-47 |
| Msa0624040 | Msa1375780 | 0.833581 | 4.911333e-56 | -8.615850e-47 |
| Msa0624040 | Msa1388520 | 0.801594 | 8.363620e-49 | -8.615850e-47 |
| Msa0624040 | Msa1394900 | 0.809746 | 1.624409e-50 | -8.615850e-47 |
| Msa0624040 | Msa1434660 | 0.812453 | 4.208419e-51 | -8.615850e-47 |
| Msa0624040 | Msa1459540 | 0.814309 | 1.645567e-51 | -8.615850e-47 |
| Msa0154220 | Msa0624040 | 0.811854 | 5.685842e-51 | -8.615850e-47 |
| Msa0154240 | Msa0624040 | 0.833597 | 4.866452e-56 | -8.615850e-47 |
| Msa0154270 | Msa0624040 | 0.844764 | 6.177170e-59 | -8.615850e-47 |
| Msa0155610 | Msa0624040 | 0.808818 | 2.568683e-50 | -8.615850e-47 |
| Msa0183560 | Msa0624040 | 0.806340 | 8.624674e-50 | -8.615850e-47 |
| Msa0187980 | Msa0624040 | 0.856503 | 3.078085e-62 | -8.615850e-47 |
| Msa0197030 | Msa0624040 | 0.801167 | 1.022952e-48 | -8.615850e-47 |
| Msa0212730 | Msa0624040 | 0.800020 | 1.753229e-48 | -8.615850e-47 |
| Msa0216220 | Msa0624040 | 0.806037 | 9.992288e-50 | -8.615850e-47 |
| Msa0224370 | Msa0624040 | 0.806939 | 6.446486e-50 | -8.615850e-47 |
| Msa0224400 | Msa0624040 | 0.822377 | 2.447396e-53 | -8.615850e-47 |
| Msa0225880 | Msa0624040 | 0.809129 | 2.203718e-50 | -8.615850e-47 |
| Msa0392050 | Msa0624040 | 0.846930 | 1.593471e-59 | -8.615850e-47 |
| Msa0401340 | Msa0624040 | 0.827151 | 1.832816e-54 | -8.615850e-47 |
| Msa0404060 | Msa0624040 | 0.844140 | 9.088908e-59 | -8.615850e-47 |
| Msa0404070 | Msa0624040 | 0.817253 | 3.629526e-52 | -8.615850e-47 |
| Msa0437550 | Msa0624040 | 0.816515 | 5.313921e-52 | -8.615850e-47 |
| Msa0448120 | Msa0624040 | 0.821587 | 3.730071e-53 | -8.615850e-47 |
| Msa0469950 | Msa0624040 | 0.847452 | 1.146294e-59 | -8.615850e-47 |
| Msa0238860 | Msa0624040 | 0.834436 | 3.001502e-56 | -8.615850e-47 |
| Msa0253160 | Msa0624040 | 0.823943 | 1.055091e-53 | -8.615850e-47 |
| Msa0260190 | Msa0624040 | 0.822471 | 2.327871e-53 | -8.615850e-47 |
| Msa0260470 | Msa0624040 | 0.803708 | 3.064358e-49 | -8.615850e-47 |
| Msa0276590 | Msa0624040 | 0.806796 | 6.912695e-50 | -8.615850e-47 |
| Msa0291940 | Msa0624040 | 0.823683 | 1.213996e-53 | -8.615850e-47 |
| Msa0303440 | Msa0624040 | 0.801558 | 8.506437e-49 | -8.615850e-47 |
| Msa0304110 | Msa0624040 | 0.814605 | 1.414723e-51 | -8.615850e-47 |
| Msa0316610 | Msa0624040 | 0.823330 | 1.467734e-53 | -8.615850e-47 |
| Msa0341190 | Msa0624040 | 0.803853 | 2.858499e-49 | -8.615850e-47 |
| Msa0344680 | Msa0624040 | 0.838770 | 2.360575e-57 | -8.615850e-47 |
| Msa0358290 | Msa0624040 | 0.806649 | 7.423394e-50 | -8.615850e-47 |
| Msa0530340 | Msa0624040 | 0.840583 | 7.967756e-58 | -8.615850e-47 |
| Msa0545230 | Msa0624040 | 0.835265 | 1.855633e-56 | -8.615850e-47 |
| Msa0546080 | Msa0624040 | 0.843593 | 1.273911e-58 | -8.615850e-47 |
| Msa0549540 | Msa0624040 | 0.827861 | 1.237846e-54 | -8.615850e-47 |
| Msa0556070 | Msa0624040 | 0.823018 | 1.735635e-53 | -8.615850e-47 |
| Msa0570680 | Msa0624040 | 0.834303 | 3.240190e-56 | -8.615850e-47 |
| Msa0584280 | Msa0624040 | 0.811732 | 6.044183e-51 | -8.615850e-47 |
| Msa0600820 | Msa0624040 | 0.807112 | 5.927231e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0624040 | MtrunA17_Chr4g0019521 | 93.162 | 117 | 7 | 1 | 3 | 118 | 2 | 118 | 1.18e-79 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0624040 | AT3G52070.2 | 43.333 | 90 | 39 | 3 | 32 | 109 | 15 | 104 | 1.85e-13 | 62.0 |
| Msa0624040 | AT3G52070.1 | 43.333 | 90 | 39 | 3 | 32 | 109 | 15 | 104 | 2.75e-13 | 61.6 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCACTCTTGGTATAGAATT+AGG | 0.267818 | 4_3:+1868140 | Msa0624040:CDS |
| CTTCTACCTTGGCAAGATAA+TGG | 0.344639 | 4_3:-1868175 | None:intergenic |
| GAGATCATTGTTGAAATTGA+AGG | 0.380819 | 4_3:+1868258 | Msa0624040:CDS |
| GTCCACAACAAAAGGGTGAT+TGG | 0.404912 | 4_3:+1868344 | Msa0624040:CDS |
| GTATAGAATTAGGACCCCTT+TGG | 0.412469 | 4_3:+1868150 | Msa0624040:CDS |
| AATAAGCACATACTAACTTC+TGG | 0.421785 | 4_3:-1868417 | None:intergenic |
| GAATTTAAGTCCACAACAAA+AGG | 0.428735 | 4_3:+1868336 | Msa0624040:CDS |
| AACTACCATACATGTACATT+AGG | 0.446681 | 4_3:-1868457 | None:intergenic |
| ATAAGCACATACTAACTTCT+GGG | 0.459372 | 4_3:-1868416 | None:intergenic |
| AAATTCTTCATCTCTTGTTG+TGG | 0.464351 | 4_3:-1868319 | None:intergenic |
| TAGTATGTGCTTATTGTGCT+TGG | 0.469587 | 4_3:+1868425 | Msa0624040:CDS |
| CACTCACCATTAGCCACTCT+TGG | 0.472156 | 4_3:+1868128 | Msa0624040:CDS |
| AGATGCATGGTCTTCTACCT+TGG | 0.492293 | 4_3:-1868186 | None:intergenic |
| TCTATACCAAGAGTGGCTAA+TGG | 0.502607 | 4_3:-1868134 | None:intergenic |
| CTATGCCTAATGTACATGTA+TGG | 0.513001 | 4_3:+1868452 | Msa0624040:CDS |
| GGCAAGATAATGGAACCAAA+GGG | 0.513637 | 4_3:-1868165 | None:intergenic |
| TTGAGAAGCTAATTGGGAAG+AGG | 0.524139 | 4_3:+1868296 | Msa0624040:CDS |
| AAGAAAGACATAGGAGCAAG+AGG | 0.530860 | 4_3:+1868229 | Msa0624040:CDS |
| TTGGTTCCATTATCTTGCCA+AGG | 0.539176 | 4_3:+1868169 | Msa0624040:CDS |
| CATGAAGAAAAGAAAGACAT+AGG | 0.544357 | 4_3:+1868220 | Msa0624040:CDS |
| TGGCAAGATAATGGAACCAA+AGG | 0.571857 | 4_3:-1868166 | None:intergenic |
| TCCTAATTCTATACCAAGAG+TGG | 0.590163 | 4_3:-1868141 | None:intergenic |
| AATTTAAGTCCACAACAAAA+GGG | 0.599106 | 4_3:+1868337 | Msa0624040:CDS |
| TGATTGGAGTTAGAGATGCA+TGG | 0.614307 | 4_3:-1868199 | None:intergenic |
| GCAAGATAATGGAACCAAAG+GGG | 0.711481 | 4_3:-1868164 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGATTTTGAGAAGCTAAT+TGG | + | chr4_3:1868289-1868308 | Msa0624040:CDS | 25.0% |
| !!! | ATGATTTTGAGAAGCTAATT+GGG | + | chr4_3:1868290-1868309 | Msa0624040:CDS | 25.0% |
| ! | AATTTAAGTCCACAACAAAA+GGG | + | chr4_3:1868337-1868356 | Msa0624040:CDS | 25.0% |
| ! | GTCTTTCTTTTCTTCATGAT+TGG | - | chr4_3:1868218-1868237 | None:intergenic | 30.0% |
| CATGAAGAAAAGAAAGACAT+AGG | + | chr4_3:1868220-1868239 | Msa0624040:CDS | 30.0% | |
| GAGATCATTGTTGAAATTGA+AGG | + | chr4_3:1868258-1868277 | Msa0624040:CDS | 30.0% | |
| AAATTCTTCATCTCTTGTTG+TGG | - | chr4_3:1868322-1868341 | None:intergenic | 30.0% | |
| GAATTTAAGTCCACAACAAA+AGG | + | chr4_3:1868336-1868355 | Msa0624040:CDS | 30.0% | |
| ATAAGCACATACTAACTTCT+GGG | - | chr4_3:1868419-1868438 | None:intergenic | 30.0% | |
| AATAAGCACATACTAACTTC+TGG | - | chr4_3:1868420-1868439 | None:intergenic | 30.0% | |
| TCCTAATTCTATACCAAGAG+TGG | - | chr4_3:1868144-1868163 | None:intergenic | 35.0% | |
| TAGTATGTGCTTATTGTGCT+TGG | + | chr4_3:1868425-1868444 | Msa0624040:CDS | 35.0% | |
| CTATGCCTAATGTACATGTA+TGG | + | chr4_3:1868452-1868471 | Msa0624040:CDS | 35.0% | |
| TCTATACCAAGAGTGGCTAA+TGG | - | chr4_3:1868137-1868156 | None:intergenic | 40.0% | |
| GCCACTCTTGGTATAGAATT+AGG | + | chr4_3:1868140-1868159 | Msa0624040:CDS | 40.0% | |
| GTATAGAATTAGGACCCCTT+TGG | + | chr4_3:1868150-1868169 | Msa0624040:CDS | 40.0% | |
| !! | GCAAGATAATGGAACCAAAG+GGG | - | chr4_3:1868167-1868186 | None:intergenic | 40.0% |
| !! | GGCAAGATAATGGAACCAAA+GGG | - | chr4_3:1868168-1868187 | None:intergenic | 40.0% |
| !! | TGGCAAGATAATGGAACCAA+AGG | - | chr4_3:1868169-1868188 | None:intergenic | 40.0% |
| TTGGTTCCATTATCTTGCCA+AGG | + | chr4_3:1868169-1868188 | Msa0624040:CDS | 40.0% | |
| CTTCTACCTTGGCAAGATAA+TGG | - | chr4_3:1868178-1868197 | None:intergenic | 40.0% | |
| TGATTGGAGTTAGAGATGCA+TGG | - | chr4_3:1868202-1868221 | None:intergenic | 40.0% | |
| AAGAAAGACATAGGAGCAAG+AGG | + | chr4_3:1868229-1868248 | Msa0624040:CDS | 40.0% | |
| TTGAGAAGCTAATTGGGAAG+AGG | + | chr4_3:1868296-1868315 | Msa0624040:CDS | 40.0% | |
| ! | TTCCAATCACCCTTTTGTTG+TGG | - | chr4_3:1868349-1868368 | None:intergenic | 40.0% |
| AGATGCATGGTCTTCTACCT+TGG | - | chr4_3:1868189-1868208 | None:intergenic | 45.0% | |
| GTCCACAACAAAAGGGTGAT+TGG | + | chr4_3:1868344-1868363 | Msa0624040:CDS | 45.0% | |
| CACTCACCATTAGCCACTCT+TGG | + | chr4_3:1868128-1868147 | Msa0624040:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 1868121 | 1868477 | 1868121 | ID=Msa0624040;Name=Msa0624040 |
| chr4_3 | mRNA | 1868121 | 1868477 | 1868121 | ID=Msa0624040-mRNA-1;Parent=Msa0624040;Name=Msa0624040-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|118 |
| chr4_3 | exon | 1868121 | 1868477 | 1868121 | ID=Msa0624040-mRNA-1:exon:491;Parent=Msa0624040-mRNA-1 |
| chr4_3 | CDS | 1868121 | 1868477 | 1868121 | ID=Msa0624040-mRNA-1:cds;Parent=Msa0624040-mRNA-1 |
| Gene Sequence |
| Protein sequence |
