AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0553050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553050 MtrunA17_Chr4g0038621 100.000 71 0 0 1 71 1 71 1.29e-49 150
Msa0553050 MtrunA17_Chr5g0413401 97.183 71 2 0 1 71 1 71 3.48e-48 146
Msa0553050 MtrunA17_Chr5g0413391 74.576 59 15 0 13 71 38 96 4.80e-29 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553050 AT1G61700.1 87.324 71 9 0 1 71 1 71 2.76e-44 137
Msa0553050 AT1G11475.1 88.732 71 8 0 1 71 1 71 9.87e-44 135

Find 36 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTGGAAAACTATTTAA+AGG 0.236886 4_1:+49833035 None:intergenic
AAACAAATGGGACTCTTATC+TGG 0.263347 4_1:-49834638 Msa0553050:CDS
GTCTGGTTCGGTATTGTTGC+AGG 0.287322 4_1:-49833264 Msa0553050:CDS
CACTGACCCTTCAGTGTAAT+CGG 0.370692 4_1:+49834603 None:intergenic
GGTAATCAGTAGTTCCTAAC+TGG 0.371023 4_1:+49833089 None:intergenic
ATTAGGTCATTGGAAACAAA+TGG 0.395280 4_1:-49834651 Msa0553050:intron
CAGTTATAGCTAGTAAAGTT+AGG 0.411822 4_1:-49833015 Msa0553050:three_prime_UTR
TTCTAGCAGCGTCTTTGAGC+GGG 0.429294 4_1:-49834852 Msa0553050:five_prime_UTR
GCAACGTAACCGACATATCT+TGG 0.430955 4_1:+49833068 None:intergenic
GGAGAAGGCTGAAGCCAGTT+AGG 0.431342 4_1:-49833103 Msa0553050:exon
TGCACTGGATGCGTTGGGTC+TGG 0.462184 4_1:-49833281 Msa0553050:CDS
TGGAAAACTATTTAAAGGAC+AGG 0.467009 4_1:+49833040 None:intergenic
CTCAAGAATTTGTCTTATGT+CGG 0.476787 4_1:+49834880 None:intergenic
CGTCTTTGAGCGGGGAAACA+AGG 0.482106 4_1:-49834843 Msa0553050:five_prime_UTR
TGGATGCGTTGGGTCTGGTT+CGG 0.497757 4_1:-49833276 Msa0553050:CDS
CCCGTTCGTTGTTTCACTTG+TGG 0.498971 4_1:-49834799 Msa0553050:CDS
CTTTACTAGCTATAACTGCT+TGG 0.514576 4_1:+49833020 None:intergenic
TATGGATTGCAGAGATGCAC+TGG 0.517566 4_1:-49833296 Msa0553050:intron
CTTCAAGCCGATTACACTGA+AGG 0.518538 4_1:-49834610 Msa0553050:CDS
CCACAAGTGAAACAACGAAC+GGG 0.522867 4_1:+49834799 None:intergenic
CAGAGATGCACTGGATGCGT+TGG 0.526584 4_1:-49833287 Msa0553050:CDS
TCGTTGTTTCACTTGTGGAA+AGG 0.534289 4_1:-49834794 Msa0553050:intron
TTAGGTCATTGGAAACAAAT+GGG 0.545252 4_1:-49834650 Msa0553050:intron
TTCAAGCCGATTACACTGAA+GGG 0.552047 4_1:-49834609 Msa0553050:intron
AGAGATGCACTGGATGCGTT+GGG 0.559881 4_1:-49833286 Msa0553050:CDS
TTTGCTTGCAGCTATGGAGA+AGG 0.578496 4_1:-49833118 Msa0553050:intron
GTTCTAGCAGCGTCTTTGAG+CGG 0.586178 4_1:-49834853 Msa0553050:five_prime_UTR
CGACATAAGACAAATTCTTG+AGG 0.597109 4_1:-49834879 Msa0553050:five_prime_UTR
TCCACAAGTGAAACAACGAA+CGG 0.616533 4_1:+49834798 None:intergenic
TCTAGCAGCGTCTTTGAGCG+GGG 0.622039 4_1:-49834851 Msa0553050:five_prime_UTR
TACTGATTACCAAGATATGT+CGG 0.624490 4_1:-49833077 Msa0553050:three_prime_UTR
TTATAGCTAGTAAAGTTAGG+AGG 0.648294 4_1:-49833012 Msa0553050:intron
GTTTCTCAATGAGATCAACA+TGG 0.660440 4_1:+49833227 None:intergenic
GGAAAACTATTTAAAGGACA+GGG 0.664065 4_1:+49833041 None:intergenic
TTTCTCAATGAGATCAACAT+GGG 0.669562 4_1:+49833228 None:intergenic
ACTTTAGTATGTGTGATGCA+AGG 0.677231 4_1:-49832932 Msa0553050:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTCTTAAAACCTTA+GGG - chr4_1:49833322-49833341 Msa0553050:intron 15.0%
!! AATTAAAAACTACAATTTAC+AGG + chr4_1:49833455-49833474 None:intergenic 15.0%
!!! CAATAGTTTATTTTTTGAAA+CGG + chr4_1:49834120-49834139 None:intergenic 15.0%
!!! AAATTTTTCTTAAAACCTTA+GGG - chr4_1:49833322-49833341 Msa0553050:intron 15.0%
!! AATTAAAAACTACAATTTAC+AGG + chr4_1:49833455-49833474 None:intergenic 15.0%
!!! CAATAGTTTATTTTTTGAAA+CGG + chr4_1:49834120-49834139 None:intergenic 15.0%
!!! TTAAACGCTTTTCAAATTTA+GGG - chr4_1:49833011-49833030 Msa0553050:intron 20.0%
!! ACGAAAATAAACAAGTATTA+AGG + chr4_1:49833262-49833281 None:intergenic 20.0%
!!! GAAATTTTTCTTAAAACCTT+AGG - chr4_1:49833321-49833340 Msa0553050:intron 20.0%
!!! TTTTAAGGTAAAATATCAAG+TGG + chr4_1:49833387-49833406 None:intergenic 20.0%
!!! TTACGCAAAGTAAAATTTTA+AGG + chr4_1:49833402-49833421 None:intergenic 20.0%
!!! AATTACTGTTGTTGTTTAAT+TGG - chr4_1:49833559-49833578 Msa0553050:intron 20.0%
!!! TGATCAAATTTTTAATGACA+AGG - chr4_1:49833711-49833730 Msa0553050:intron 20.0%
!! CAATGATTAAATTTATGTCT+TGG - chr4_1:49833774-49833793 Msa0553050:intron 20.0%
!! CGTTTCAAAAAATAAACTAT+TGG - chr4_1:49834118-49834137 Msa0553050:intron 20.0%
!! AAGAGATCTAGTATATTTAA+GGG + chr4_1:49834342-49834361 None:intergenic 20.0%
!! AAAGAGATCTAGTATATTTA+AGG + chr4_1:49834343-49834362 None:intergenic 20.0%
!! AATTTAAACTAATATTTGCC+AGG + chr4_1:49834387-49834406 None:intergenic 20.0%
!! ATTTCGTAGTATTATCATAA+TGG - chr4_1:49834404-49834423 Msa0553050:intron 20.0%
!!! TTAAACGCTTTTCAAATTTA+GGG - chr4_1:49833011-49833030 Msa0553050:intron 20.0%
!! ACGAAAATAAACAAGTATTA+AGG + chr4_1:49833262-49833281 None:intergenic 20.0%
!!! GAAATTTTTCTTAAAACCTT+AGG - chr4_1:49833321-49833340 Msa0553050:intron 20.0%
!!! TTTTAAGGTAAAATATCAAG+TGG + chr4_1:49833387-49833406 None:intergenic 20.0%
!!! TTACGCAAAGTAAAATTTTA+AGG + chr4_1:49833402-49833421 None:intergenic 20.0%
!!! AATTACTGTTGTTGTTTAAT+TGG - chr4_1:49833559-49833578 Msa0553050:intron 20.0%
!!! TGATCAAATTTTTAATGACA+AGG - chr4_1:49833711-49833730 Msa0553050:intron 20.0%
!! CAATGATTAAATTTATGTCT+TGG - chr4_1:49833774-49833793 Msa0553050:intron 20.0%
!! CGTTTCAAAAAATAAACTAT+TGG - chr4_1:49834118-49834137 Msa0553050:intron 20.0%
!! AAGAGATCTAGTATATTTAA+GGG + chr4_1:49834342-49834361 None:intergenic 20.0%
!! AAAGAGATCTAGTATATTTA+AGG + chr4_1:49834343-49834362 None:intergenic 20.0%
!! AATTTAAACTAATATTTGCC+AGG + chr4_1:49834387-49834406 None:intergenic 20.0%
!! ATTTCGTAGTATTATCATAA+TGG - chr4_1:49834404-49834423 Msa0553050:intron 20.0%
!! GTTAAACGCTTTTCAAATTT+AGG - chr4_1:49833010-49833029 Msa0553050:intron 25.0%
!!! TTTTCTTATTTCTGCATGTA+GGG - chr4_1:49833160-49833179 Msa0553050:intron 25.0%
! AAGAAAAGAATCATGCTAAT+TGG - chr4_1:49833299-49833318 Msa0553050:intron 25.0%
! TAATAGATATTGTGGAATAG+AGG - chr4_1:49833419-49833438 Msa0553050:intron 25.0%
!!! TGTTGTTGTTTAATTGGTTA+GGG - chr4_1:49833565-49833584 Msa0553050:intron 25.0%
!! TCAAATTTTTAATGACAAGG+TGG - chr4_1:49833714-49833733 Msa0553050:intron 25.0%
! TTAAATTTATGTCTTGGAGA+TGG - chr4_1:49833780-49833799 Msa0553050:intron 25.0%
!! AACCTTGTTTGTATTATGAA+TGG - chr4_1:49833809-49833828 Msa0553050:intron 25.0%
! ATAAAACGAATTGCTTCAAT+AGG - chr4_1:49833839-49833858 Msa0553050:intron 25.0%
! TTTGATCCTAATTGTGTATT+CGG - chr4_1:49834038-49834057 Msa0553050:intron 25.0%
!! ATTAACAATTTTATCGCATC+TGG - chr4_1:49834267-49834286 Msa0553050:intron 25.0%
! AGTATTATCATAATGGTGTA+TGG - chr4_1:49834411-49834430 Msa0553050:intron 25.0%
! TGACAACACAGAATAATATA+GGG + chr4_1:49834551-49834570 None:intergenic 25.0%
! TTGACAACACAGAATAATAT+AGG + chr4_1:49834552-49834571 None:intergenic 25.0%
!! TGTCAATTTTCTAATGTCAT+TGG - chr4_1:49834566-49834585 Msa0553050:intron 25.0%
!! GTCAATTTTCTAATGTCATT+GGG - chr4_1:49834567-49834586 Msa0553050:intron 25.0%
!! GTTAAACGCTTTTCAAATTT+AGG - chr4_1:49833010-49833029 Msa0553050:intron 25.0%
!!! TTTTCTTATTTCTGCATGTA+GGG - chr4_1:49833160-49833179 Msa0553050:intron 25.0%
! AAGAAAAGAATCATGCTAAT+TGG - chr4_1:49833299-49833318 Msa0553050:intron 25.0%
! TAATAGATATTGTGGAATAG+AGG - chr4_1:49833419-49833438 Msa0553050:intron 25.0%
!!! TGTTGTTGTTTAATTGGTTA+GGG - chr4_1:49833565-49833584 Msa0553050:intron 25.0%
!! TCAAATTTTTAATGACAAGG+TGG - chr4_1:49833714-49833733 Msa0553050:intron 25.0%
! TTAAATTTATGTCTTGGAGA+TGG - chr4_1:49833780-49833799 Msa0553050:intron 25.0%
!! AACCTTGTTTGTATTATGAA+TGG - chr4_1:49833809-49833828 Msa0553050:intron 25.0%
! ATAAAACGAATTGCTTCAAT+AGG - chr4_1:49833839-49833858 Msa0553050:intron 25.0%
! TTTGATCCTAATTGTGTATT+CGG - chr4_1:49834038-49834057 Msa0553050:intron 25.0%
!! ATTAACAATTTTATCGCATC+TGG - chr4_1:49834267-49834286 Msa0553050:intron 25.0%
! AGTATTATCATAATGGTGTA+TGG - chr4_1:49834411-49834430 Msa0553050:intron 25.0%
! TGACAACACAGAATAATATA+GGG + chr4_1:49834551-49834570 None:intergenic 25.0%
! TTGACAACACAGAATAATAT+AGG + chr4_1:49834552-49834571 None:intergenic 25.0%
!! TGTCAATTTTCTAATGTCAT+TGG - chr4_1:49834566-49834585 Msa0553050:intron 25.0%
!! GTCAATTTTCTAATGTCATT+GGG - chr4_1:49834567-49834586 Msa0553050:intron 25.0%
CTCAAGAATTTGTCTTATGT+CGG + chr4_1:49832848-49832867 None:intergenic 30.0%
ATGCAGAGAAAAAAAGAAGA+AGG + chr4_1:49832970-49832989 None:intergenic 30.0%
!!! CGTTTTATTTTCTCCCAATT+AGG - chr4_1:49833057-49833076 Msa0553050:three_prime_UTR 30.0%
TTGTTTCCAATGACCTAATT+GGG + chr4_1:49833073-49833092 None:intergenic 30.0%
TTTGTTTCCAATGACCTAAT+TGG + chr4_1:49833074-49833093 None:intergenic 30.0%
ATTAGGTCATTGGAAACAAA+TGG - chr4_1:49833074-49833093 Msa0553050:three_prime_UTR 30.0%
TTAGGTCATTGGAAACAAAT+GGG - chr4_1:49833075-49833094 Msa0553050:three_prime_UTR 30.0%
TAAGAAAAGAGAAAGGATCA+AGG + chr4_1:49833148-49833167 None:intergenic 30.0%
GCAGAAATAAGAAAAGAGAA+AGG + chr4_1:49833155-49833174 None:intergenic 30.0%
!!! CTTTTCTTATTTCTGCATGT+AGG - chr4_1:49833159-49833178 Msa0553050:intron 30.0%
ATAATCACCACACAAAAACT+TGG + chr4_1:49833351-49833370 None:intergenic 30.0%
ACTTTGCGTAATAGATATTG+TGG - chr4_1:49833411-49833430 Msa0553050:intron 30.0%
!! CTGTTGTTGTTTAATTGGTT+AGG - chr4_1:49833564-49833583 Msa0553050:intron 30.0%
! AATTTTTAATGACAAGGTGG+CGG - chr4_1:49833717-49833736 Msa0553050:intron 30.0%
!!! CGGTTGATCTAGAAATTTTT+TGG - chr4_1:49833737-49833756 Msa0553050:intron 30.0%
CACCATTCATAATACAAACA+AGG + chr4_1:49833814-49833833 None:intergenic 30.0%
! GCTTCTTATTACTTCCTTTT+GGG - chr4_1:49833960-49833979 Msa0553050:intron 30.0%
! CTTCTTATTACTTCCTTTTG+GGG - chr4_1:49833961-49833980 Msa0553050:intron 30.0%
! AGGTTTTTAAACTAGTTGAG+AGG - chr4_1:49834193-49834212 Msa0553050:intron 30.0%
! GGTTTTTAAACTAGTTGAGA+GGG - chr4_1:49834194-49834213 Msa0553050:intron 30.0%
AAATAAATGTGATTGCAGAG+CGG - chr4_1:49834218-49834237 Msa0553050:intron 30.0%
GCATTTCAAAATTTATCTCC+TGG - chr4_1:49834366-49834385 Msa0553050:intron 30.0%
TTTCTCAATGAGATCAACAT+GGG + chr4_1:49834500-49834519 None:intergenic 30.0%
! TACTGATTACCAAGATATGT+CGG - chr4_1:49834648-49834667 Msa0553050:CDS 30.0%
GGAAAACTATTTAAAGGACA+GGG + chr4_1:49834687-49834706 None:intergenic 30.0%
TGGAAAACTATTTAAAGGAC+AGG + chr4_1:49834688-49834707 None:intergenic 30.0%
CTGCTTGGAAAACTATTTAA+AGG + chr4_1:49834693-49834712 None:intergenic 30.0%
CAGTTATAGCTAGTAAAGTT+AGG - chr4_1:49834710-49834729 Msa0553050:intron 30.0%
TTATAGCTAGTAAAGTTAGG+AGG - chr4_1:49834713-49834732 Msa0553050:intron 30.0%
! TGACAGCTTTTCTATGAAAA+TGG - chr4_1:49834859-49834878 Msa0553050:five_prime_UTR 30.0%
! GACAGCTTTTCTATGAAAAT+GGG - chr4_1:49834860-49834879 Msa0553050:five_prime_UTR 30.0%
TCATAATTGATACTGATGCT+TGG - chr4_1:49834896-49834915 Msa0553050:five_prime_UTR 30.0%
CTCAAGAATTTGTCTTATGT+CGG + chr4_1:49832848-49832867 None:intergenic 30.0%
ATGCAGAGAAAAAAAGAAGA+AGG + chr4_1:49832970-49832989 None:intergenic 30.0%
!!! CGTTTTATTTTCTCCCAATT+AGG - chr4_1:49833057-49833076 Msa0553050:three_prime_UTR 30.0%
TTGTTTCCAATGACCTAATT+GGG + chr4_1:49833073-49833092 None:intergenic 30.0%
TTTGTTTCCAATGACCTAAT+TGG + chr4_1:49833074-49833093 None:intergenic 30.0%
ATTAGGTCATTGGAAACAAA+TGG - chr4_1:49833074-49833093 Msa0553050:three_prime_UTR 30.0%
TTAGGTCATTGGAAACAAAT+GGG - chr4_1:49833075-49833094 Msa0553050:three_prime_UTR 30.0%
TAAGAAAAGAGAAAGGATCA+AGG + chr4_1:49833148-49833167 None:intergenic 30.0%
GCAGAAATAAGAAAAGAGAA+AGG + chr4_1:49833155-49833174 None:intergenic 30.0%
!!! CTTTTCTTATTTCTGCATGT+AGG - chr4_1:49833159-49833178 Msa0553050:intron 30.0%
ATAATCACCACACAAAAACT+TGG + chr4_1:49833351-49833370 None:intergenic 30.0%
ACTTTGCGTAATAGATATTG+TGG - chr4_1:49833411-49833430 Msa0553050:intron 30.0%
!! CTGTTGTTGTTTAATTGGTT+AGG - chr4_1:49833564-49833583 Msa0553050:intron 30.0%
! AATTTTTAATGACAAGGTGG+CGG - chr4_1:49833717-49833736 Msa0553050:intron 30.0%
!!! CGGTTGATCTAGAAATTTTT+TGG - chr4_1:49833737-49833756 Msa0553050:intron 30.0%
CACCATTCATAATACAAACA+AGG + chr4_1:49833814-49833833 None:intergenic 30.0%
! GCTTCTTATTACTTCCTTTT+GGG - chr4_1:49833960-49833979 Msa0553050:intron 30.0%
! CTTCTTATTACTTCCTTTTG+GGG - chr4_1:49833961-49833980 Msa0553050:intron 30.0%
! AGGTTTTTAAACTAGTTGAG+AGG - chr4_1:49834193-49834212 Msa0553050:intron 30.0%
! GGTTTTTAAACTAGTTGAGA+GGG - chr4_1:49834194-49834213 Msa0553050:intron 30.0%
AAATAAATGTGATTGCAGAG+CGG - chr4_1:49834218-49834237 Msa0553050:intron 30.0%
GCATTTCAAAATTTATCTCC+TGG - chr4_1:49834366-49834385 Msa0553050:intron 30.0%
TTTCTCAATGAGATCAACAT+GGG + chr4_1:49834500-49834519 None:intergenic 30.0%
! TACTGATTACCAAGATATGT+CGG - chr4_1:49834648-49834667 Msa0553050:CDS 30.0%
GGAAAACTATTTAAAGGACA+GGG + chr4_1:49834687-49834706 None:intergenic 30.0%
TGGAAAACTATTTAAAGGAC+AGG + chr4_1:49834688-49834707 None:intergenic 30.0%
CTGCTTGGAAAACTATTTAA+AGG + chr4_1:49834693-49834712 None:intergenic 30.0%
CAGTTATAGCTAGTAAAGTT+AGG - chr4_1:49834710-49834729 Msa0553050:intron 30.0%
TTATAGCTAGTAAAGTTAGG+AGG - chr4_1:49834713-49834732 Msa0553050:intron 30.0%
! TGACAGCTTTTCTATGAAAA+TGG - chr4_1:49834859-49834878 Msa0553050:five_prime_UTR 30.0%
! GACAGCTTTTCTATGAAAAT+GGG - chr4_1:49834860-49834879 Msa0553050:five_prime_UTR 30.0%
CGACATAAGACAAATTCTTG+AGG - chr4_1:49832846-49832865 Msa0553050:three_prime_UTR 35.0%
! TTTTCTCCCAATTAGGTCAT+TGG - chr4_1:49833064-49833083 Msa0553050:three_prime_UTR 35.0%
AAACAAATGGGACTCTTATC+TGG - chr4_1:49833087-49833106 Msa0553050:exon 35.0%
! ACAAAAACTTGGTAACCCTA+AGG + chr4_1:49833340-49833359 None:intergenic 35.0%
GTTGTTTAATTGGTTAGGGT+TGG - chr4_1:49833569-49833588 Msa0553050:intron 35.0%
! GTTACACCTCAAAAGTTTTG+AGG - chr4_1:49833597-49833616 Msa0553050:intron 35.0%
! TCAACACCTCAAAACTTTTG+AGG + chr4_1:49833606-49833625 None:intergenic 35.0%
!!! AAAAGTTTTGAGGTGTTGAG+TGG - chr4_1:49833607-49833626 Msa0553050:intron 35.0%
!!! AAAGTTTTGAGGTGTTGAGT+GGG - chr4_1:49833608-49833627 Msa0553050:intron 35.0%
! AGAAAATGCGGAAAGGTTTT+TGG - chr4_1:49833644-49833663 Msa0553050:intron 35.0%
!!! GGAAAGGTTTTTGGCTTATT+TGG - chr4_1:49833653-49833672 Msa0553050:intron 35.0%
TTATTTGGCACGCAACAATT+TGG - chr4_1:49833668-49833687 Msa0553050:intron 35.0%
TATTTGGCACGCAACAATTT+GGG - chr4_1:49833669-49833688 Msa0553050:intron 35.0%
AAACGAATTGCTTCAATAGG+CGG - chr4_1:49833842-49833861 Msa0553050:intron 35.0%
CTTTCTGTTGTGTGTAAACT+TGG - chr4_1:49833918-49833937 Msa0553050:intron 35.0%
! GGCTTCTTATTACTTCCTTT+TGG - chr4_1:49833959-49833978 Msa0553050:intron 35.0%
!! TTATTACTTCCTTTTGGGGA+TGG - chr4_1:49833965-49833984 Msa0553050:intron 35.0%
!! TATTACTTCCTTTTGGGGAT+GGG - chr4_1:49833966-49833985 Msa0553050:intron 35.0%
TGGAAACCGAATACACAATT+AGG + chr4_1:49834047-49834066 None:intergenic 35.0%
AATAAATGTGATTGCAGAGC+GGG - chr4_1:49834219-49834238 Msa0553050:intron 35.0%
GTTTCTCAATGAGATCAACA+TGG + chr4_1:49834501-49834520 None:intergenic 35.0%
CTTTACTAGCTATAACTGCT+TGG + chr4_1:49834708-49834727 None:intergenic 35.0%
!! GTAAAGTTAGGAGGTATTTG+TGG - chr4_1:49834722-49834741 Msa0553050:intron 35.0%
!! AGGAGGTATTTGTGGTTATT+AGG - chr4_1:49834730-49834749 Msa0553050:intron 35.0%
AGGTATTTGTGGTTATTAGG+AGG - chr4_1:49834733-49834752 Msa0553050:intron 35.0%
ACTTTAGTATGTGTGATGCA+AGG - chr4_1:49834793-49834812 Msa0553050:intron 35.0%
CGACATAAGACAAATTCTTG+AGG - chr4_1:49832846-49832865 Msa0553050:three_prime_UTR 35.0%
! TTTTCTCCCAATTAGGTCAT+TGG - chr4_1:49833064-49833083 Msa0553050:three_prime_UTR 35.0%
AAACAAATGGGACTCTTATC+TGG - chr4_1:49833087-49833106 Msa0553050:exon 35.0%
! ACAAAAACTTGGTAACCCTA+AGG + chr4_1:49833340-49833359 None:intergenic 35.0%
GTTGTTTAATTGGTTAGGGT+TGG - chr4_1:49833569-49833588 Msa0553050:intron 35.0%
! GTTACACCTCAAAAGTTTTG+AGG - chr4_1:49833597-49833616 Msa0553050:intron 35.0%
! TCAACACCTCAAAACTTTTG+AGG + chr4_1:49833606-49833625 None:intergenic 35.0%
!!! AAAAGTTTTGAGGTGTTGAG+TGG - chr4_1:49833607-49833626 Msa0553050:intron 35.0%
!!! AAAGTTTTGAGGTGTTGAGT+GGG - chr4_1:49833608-49833627 Msa0553050:intron 35.0%
! AGAAAATGCGGAAAGGTTTT+TGG - chr4_1:49833644-49833663 Msa0553050:intron 35.0%
!!! GGAAAGGTTTTTGGCTTATT+TGG - chr4_1:49833653-49833672 Msa0553050:intron 35.0%
TTATTTGGCACGCAACAATT+TGG - chr4_1:49833668-49833687 Msa0553050:intron 35.0%
TATTTGGCACGCAACAATTT+GGG - chr4_1:49833669-49833688 Msa0553050:intron 35.0%
AAACGAATTGCTTCAATAGG+CGG - chr4_1:49833842-49833861 Msa0553050:intron 35.0%
CTTTCTGTTGTGTGTAAACT+TGG - chr4_1:49833918-49833937 Msa0553050:intron 35.0%
! GGCTTCTTATTACTTCCTTT+TGG - chr4_1:49833959-49833978 Msa0553050:intron 35.0%
!! TTATTACTTCCTTTTGGGGA+TGG - chr4_1:49833965-49833984 Msa0553050:intron 35.0%
!! TATTACTTCCTTTTGGGGAT+GGG - chr4_1:49833966-49833985 Msa0553050:intron 35.0%
TGGAAACCGAATACACAATT+AGG + chr4_1:49834047-49834066 None:intergenic 35.0%
AATAAATGTGATTGCAGAGC+GGG - chr4_1:49834219-49834238 Msa0553050:intron 35.0%
GTTTCTCAATGAGATCAACA+TGG + chr4_1:49834501-49834520 None:intergenic 35.0%
CTTTACTAGCTATAACTGCT+TGG + chr4_1:49834708-49834727 None:intergenic 35.0%
!! GTAAAGTTAGGAGGTATTTG+TGG - chr4_1:49834722-49834741 Msa0553050:intron 35.0%
!! AGGAGGTATTTGTGGTTATT+AGG - chr4_1:49834730-49834749 Msa0553050:intron 35.0%
AGGTATTTGTGGTTATTAGG+AGG - chr4_1:49834733-49834752 Msa0553050:intron 35.0%
ACTTTAGTATGTGTGATGCA+AGG - chr4_1:49834793-49834812 Msa0553050:intron 35.0%
TCCACAAGTGAAACAACGAA+CGG + chr4_1:49832930-49832949 None:intergenic 40.0%
! TCGTTGTTTCACTTGTGGAA+AGG - chr4_1:49832931-49832950 Msa0553050:three_prime_UTR 40.0%
TTCAAGCCGATTACACTGAA+GGG - chr4_1:49833116-49833135 Msa0553050:intron 40.0%
! AGGGTTACCAAGTTTTTGTG+TGG - chr4_1:49833341-49833360 Msa0553050:intron 40.0%
TATTGTGGAATAGAGGAGCA+TGG - chr4_1:49833426-49833445 Msa0553050:intron 40.0%
! GCACTGACACTTATAATCGA+AGG - chr4_1:49833480-49833499 Msa0553050:intron 40.0%
!! TAATTGGTTAGGGTTGGTTG+TGG - chr4_1:49833575-49833594 Msa0553050:intron 40.0%
! GAGCATTGTGTTAGACTTAG+AGG - chr4_1:49833883-49833902 Msa0553050:intron 40.0%
TGTTGTGTGTAAACTTGGAC+AGG - chr4_1:49833923-49833942 Msa0553050:intron 40.0%
GTTGTGTGTAAACTTGGACA+GGG - chr4_1:49833924-49833943 Msa0553050:intron 40.0%
TTGTGTGTAAACTTGGACAG+GGG - chr4_1:49833925-49833944 Msa0553050:intron 40.0%
!!! TGGACAGGGGTTGTTTTTAT+TGG - chr4_1:49833938-49833957 Msa0553050:intron 40.0%
! GGGTTTTTGTCTATGCGTTT+AGG - chr4_1:49833986-49834005 Msa0553050:intron 40.0%
ACGAAACACTGACACATACA+CGG + chr4_1:49834173-49834192 None:intergenic 40.0%
TGTATGTGTCAGTGTTTCGT+AGG - chr4_1:49834173-49834192 Msa0553050:intron 40.0%
ATATTTAAGGGACGCAGTCA+GGG + chr4_1:49834330-49834349 None:intergenic 40.0%
TATATTTAAGGGACGCAGTC+AGG + chr4_1:49834331-49834350 None:intergenic 40.0%
CATACATTTGCTTGCAGCTA+TGG - chr4_1:49834601-49834620 Msa0553050:intron 40.0%
GGTAATCAGTAGTTCCTAAC+TGG + chr4_1:49834639-49834658 None:intergenic 40.0%
TCCACAAGTGAAACAACGAA+CGG + chr4_1:49832930-49832949 None:intergenic 40.0%
! TCGTTGTTTCACTTGTGGAA+AGG - chr4_1:49832931-49832950 Msa0553050:three_prime_UTR 40.0%
TTCAAGCCGATTACACTGAA+GGG - chr4_1:49833116-49833135 Msa0553050:intron 40.0%
! AGGGTTACCAAGTTTTTGTG+TGG - chr4_1:49833341-49833360 Msa0553050:intron 40.0%
TATTGTGGAATAGAGGAGCA+TGG - chr4_1:49833426-49833445 Msa0553050:intron 40.0%
! GCACTGACACTTATAATCGA+AGG - chr4_1:49833480-49833499 Msa0553050:intron 40.0%
!! TAATTGGTTAGGGTTGGTTG+TGG - chr4_1:49833575-49833594 Msa0553050:intron 40.0%
! GAGCATTGTGTTAGACTTAG+AGG - chr4_1:49833883-49833902 Msa0553050:intron 40.0%
TGTTGTGTGTAAACTTGGAC+AGG - chr4_1:49833923-49833942 Msa0553050:intron 40.0%
GTTGTGTGTAAACTTGGACA+GGG - chr4_1:49833924-49833943 Msa0553050:intron 40.0%
TTGTGTGTAAACTTGGACAG+GGG - chr4_1:49833925-49833944 Msa0553050:intron 40.0%
!!! TGGACAGGGGTTGTTTTTAT+TGG - chr4_1:49833938-49833957 Msa0553050:intron 40.0%
! GGGTTTTTGTCTATGCGTTT+AGG - chr4_1:49833986-49834005 Msa0553050:intron 40.0%
ACGAAACACTGACACATACA+CGG + chr4_1:49834173-49834192 None:intergenic 40.0%
TGTATGTGTCAGTGTTTCGT+AGG - chr4_1:49834173-49834192 Msa0553050:intron 40.0%
ATATTTAAGGGACGCAGTCA+GGG + chr4_1:49834330-49834349 None:intergenic 40.0%
TATATTTAAGGGACGCAGTC+AGG + chr4_1:49834331-49834350 None:intergenic 40.0%
CATACATTTGCTTGCAGCTA+TGG - chr4_1:49834601-49834620 Msa0553050:intron 40.0%
GGTAATCAGTAGTTCCTAAC+TGG + chr4_1:49834639-49834658 None:intergenic 40.0%
CCACAAGTGAAACAACGAAC+GGG + chr4_1:49832929-49832948 None:intergenic 45.0%
CTTCAAGCCGATTACACTGA+AGG - chr4_1:49833115-49833134 Msa0553050:intron 45.0%
CACTGACCCTTCAGTGTAAT+CGG + chr4_1:49833125-49833144 None:intergenic 45.0%
!! TTTGAGGTGTTGAGTGGGTT+AGG - chr4_1:49833613-49833632 Msa0553050:intron 45.0%
!! TTGAGGTGTTGAGTGGGTTA+GGG - chr4_1:49833614-49833633 Msa0553050:intron 45.0%
TAGGGAGTGGAGAGAAAATG+CGG - chr4_1:49833632-49833651 Msa0553050:intron 45.0%
AGTGGAGAGAAAATGCGGAA+AGG - chr4_1:49833637-49833656 Msa0553050:intron 45.0%
ACGCAACAATTTGGGTGCTT+TGG - chr4_1:49833677-49833696 Msa0553050:intron 45.0%
CACAACAGAAAGCAAACAGC+AGG + chr4_1:49833910-49833929 None:intergenic 45.0%
GACAAAAACCCATCCCCAAA+AGG + chr4_1:49833977-49833996 None:intergenic 45.0%
! GTTTAGGTGTTGCCTTGTTG+TGG - chr4_1:49834002-49834021 Msa0553050:intron 45.0%
AAAACAGCCCAACCACAACA+AGG + chr4_1:49834017-49834036 None:intergenic 45.0%
TAAAAGACGTGCAAGAGAGC+TGG + chr4_1:49834067-49834086 None:intergenic 45.0%
! TATGGATTGCAGAGATGCAC+TGG - chr4_1:49834429-49834448 Msa0553050:intron 45.0%
TTTGCTTGCAGCTATGGAGA+AGG - chr4_1:49834607-49834626 Msa0553050:intron 45.0%
GCAACGTAACCGACATATCT+TGG + chr4_1:49834660-49834679 None:intergenic 45.0%
CCACAAGTGAAACAACGAAC+GGG + chr4_1:49832929-49832948 None:intergenic 45.0%
CTTCAAGCCGATTACACTGA+AGG - chr4_1:49833115-49833134 Msa0553050:intron 45.0%
CACTGACCCTTCAGTGTAAT+CGG + chr4_1:49833125-49833144 None:intergenic 45.0%
!! TTTGAGGTGTTGAGTGGGTT+AGG - chr4_1:49833613-49833632 Msa0553050:intron 45.0%
!! TTGAGGTGTTGAGTGGGTTA+GGG - chr4_1:49833614-49833633 Msa0553050:intron 45.0%
TAGGGAGTGGAGAGAAAATG+CGG - chr4_1:49833632-49833651 Msa0553050:intron 45.0%
AGTGGAGAGAAAATGCGGAA+AGG - chr4_1:49833637-49833656 Msa0553050:intron 45.0%
ACGCAACAATTTGGGTGCTT+TGG - chr4_1:49833677-49833696 Msa0553050:intron 45.0%
CACAACAGAAAGCAAACAGC+AGG + chr4_1:49833910-49833929 None:intergenic 45.0%
GACAAAAACCCATCCCCAAA+AGG + chr4_1:49833977-49833996 None:intergenic 45.0%
! GTTTAGGTGTTGCCTTGTTG+TGG - chr4_1:49834002-49834021 Msa0553050:intron 45.0%
AAAACAGCCCAACCACAACA+AGG + chr4_1:49834017-49834036 None:intergenic 45.0%
TAAAAGACGTGCAAGAGAGC+TGG + chr4_1:49834067-49834086 None:intergenic 45.0%
! TATGGATTGCAGAGATGCAC+TGG - chr4_1:49834429-49834448 Msa0553050:intron 45.0%
TTTGCTTGCAGCTATGGAGA+AGG - chr4_1:49834607-49834626 Msa0553050:intron 45.0%
GCAACGTAACCGACATATCT+TGG + chr4_1:49834660-49834679 None:intergenic 45.0%
!!! TTTTGATTAATAAAAAAAAA+GGG + chr4_1:49833191-49833210 None:intergenic 5.0%
!!! ATTTTGATTAATAAAAAAAA+AGG + chr4_1:49833192-49833211 None:intergenic 5.0%
!!! TTTTGATTAATAAAAAAAAA+GGG + chr4_1:49833191-49833210 None:intergenic 5.0%
!!! ATTTTGATTAATAAAAAAAA+AGG + chr4_1:49833192-49833211 None:intergenic 5.0%
GTTCTAGCAGCGTCTTTGAG+CGG - chr4_1:49832872-49832891 Msa0553050:three_prime_UTR 50.0%
TTCTAGCAGCGTCTTTGAGC+GGG - chr4_1:49832873-49832892 Msa0553050:three_prime_UTR 50.0%
! CCCGTTCGTTGTTTCACTTG+TGG - chr4_1:49832926-49832945 Msa0553050:three_prime_UTR 50.0%
CGAATTGCTTCAATAGGCGG+TGG - chr4_1:49833845-49833864 Msa0553050:intron 50.0%
ATTGCTTCAATAGGCGGTGG+TGG - chr4_1:49833848-49833867 Msa0553050:intron 50.0%
TTGCTTCAATAGGCGGTGGT+GGG - chr4_1:49833849-49833868 Msa0553050:intron 50.0%
! AGGTGTTGCCTTGTTGTGGT+TGG - chr4_1:49834006-49834025 Msa0553050:intron 50.0%
! GGTGTTGCCTTGTTGTGGTT+GGG - chr4_1:49834007-49834026 Msa0553050:intron 50.0%
TGGTGTATCAGCGTCAATGC+TGG - chr4_1:49834138-49834157 Msa0553050:intron 50.0%
GGTGTATCAGCGTCAATGCT+GGG - chr4_1:49834139-49834158 Msa0553050:intron 50.0%
ATCAGCGTCAATGCTGGGTA+TGG - chr4_1:49834144-49834163 Msa0553050:intron 50.0%
AGAGATGCACTGGATGCGTT+GGG - chr4_1:49834439-49834458 Msa0553050:intron 50.0%
!! GTCTGGTTCGGTATTGTTGC+AGG - chr4_1:49834461-49834480 Msa0553050:intron 50.0%
GTTCTAGCAGCGTCTTTGAG+CGG - chr4_1:49832872-49832891 Msa0553050:three_prime_UTR 50.0%
TTCTAGCAGCGTCTTTGAGC+GGG - chr4_1:49832873-49832892 Msa0553050:three_prime_UTR 50.0%
! CCCGTTCGTTGTTTCACTTG+TGG - chr4_1:49832926-49832945 Msa0553050:three_prime_UTR 50.0%
CGAATTGCTTCAATAGGCGG+TGG - chr4_1:49833845-49833864 Msa0553050:intron 50.0%
ATTGCTTCAATAGGCGGTGG+TGG - chr4_1:49833848-49833867 Msa0553050:intron 50.0%
TTGCTTCAATAGGCGGTGGT+GGG - chr4_1:49833849-49833868 Msa0553050:intron 50.0%
! AGGTGTTGCCTTGTTGTGGT+TGG - chr4_1:49834006-49834025 Msa0553050:intron 50.0%
! GGTGTTGCCTTGTTGTGGTT+GGG - chr4_1:49834007-49834026 Msa0553050:intron 50.0%
TGGTGTATCAGCGTCAATGC+TGG - chr4_1:49834138-49834157 Msa0553050:intron 50.0%
GGTGTATCAGCGTCAATGCT+GGG - chr4_1:49834139-49834158 Msa0553050:intron 50.0%
ATCAGCGTCAATGCTGGGTA+TGG - chr4_1:49834144-49834163 Msa0553050:intron 50.0%
AGAGATGCACTGGATGCGTT+GGG - chr4_1:49834439-49834458 Msa0553050:intron 50.0%
!! GTCTGGTTCGGTATTGTTGC+AGG - chr4_1:49834461-49834480 Msa0553050:intron 50.0%
TCTAGCAGCGTCTTTGAGCG+GGG - chr4_1:49832874-49832893 Msa0553050:three_prime_UTR 55.0%
CGTCTTTGAGCGGGGAAACA+AGG - chr4_1:49832882-49832901 Msa0553050:three_prime_UTR 55.0%
!! GTGTTGAGTGGGTTAGGGAG+TGG - chr4_1:49833619-49833638 Msa0553050:intron 55.0%
CAGAGATGCACTGGATGCGT+TGG - chr4_1:49834438-49834457 Msa0553050:intron 55.0%
TGGATGCGTTGGGTCTGGTT+CGG - chr4_1:49834449-49834468 Msa0553050:intron 55.0%
GGAGAAGGCTGAAGCCAGTT+AGG - chr4_1:49834622-49834641 Msa0553050:CDS 55.0%
TCTAGCAGCGTCTTTGAGCG+GGG - chr4_1:49832874-49832893 Msa0553050:three_prime_UTR 55.0%
CGTCTTTGAGCGGGGAAACA+AGG - chr4_1:49832882-49832901 Msa0553050:three_prime_UTR 55.0%
!! GTGTTGAGTGGGTTAGGGAG+TGG - chr4_1:49833619-49833638 Msa0553050:intron 55.0%
CAGAGATGCACTGGATGCGT+TGG - chr4_1:49834438-49834457 Msa0553050:intron 55.0%
TGGATGCGTTGGGTCTGGTT+CGG - chr4_1:49834449-49834468 Msa0553050:intron 55.0%
GGAGAAGGCTGAAGCCAGTT+AGG - chr4_1:49834622-49834641 Msa0553050:CDS 55.0%
!! CTTCAATAGGCGGTGGTGGG+TGG - chr4_1:49833852-49833871 Msa0553050:intron 60.0%
TGCACTGGATGCGTTGGGTC+TGG - chr4_1:49834444-49834463 Msa0553050:intron 60.0%
!! CTTCAATAGGCGGTGGTGGG+TGG - chr4_1:49833852-49833871 Msa0553050:intron 60.0%
TGCACTGGATGCGTTGGGTC+TGG - chr4_1:49834444-49834463 Msa0553050:intron 60.0%
!! GCGGTGGTGGGTGGTTGTGT+TGG - chr4_1:49833861-49833880 Msa0553050:intron 65.0%
!! GCGGTGGTGGGTGGTTGTGT+TGG - chr4_1:49833861-49833880 Msa0553050:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 49832830 49834917 49832830 ID=Msa0553050;Name=Msa0553050
chr4_1 mRNA 49832830 49834917 49832830 ID=Msa0553050-mRNA-1;Parent=Msa0553050;Name=Msa0553050-mRNA-1;_AED=0.18;_eAED=0.18;_QI=87|1|1|1|0.75|0.6|5|241|71
chr4_1 exon 49832830 49832979 49832830 ID=Msa0553050-mRNA-1:exon:14093;Parent=Msa0553050-mRNA-1
chr4_1 exon 49833013 49833129 49833013 ID=Msa0553050-mRNA-1:exon:14092;Parent=Msa0553050-mRNA-1
chr4_1 exon 49833212 49833306 49833212 ID=Msa0553050-mRNA-1:exon:14091;Parent=Msa0553050-mRNA-1
chr4_1 exon 49834610 49834668 49834610 ID=Msa0553050-mRNA-1:exon:14090;Parent=Msa0553050-mRNA-1
chr4_1 exon 49834795 49834917 49834795 ID=Msa0553050-mRNA-1:exon:14089;Parent=Msa0553050-mRNA-1
chr4_1 five_prime_UTR 49834831 49834917 49834831 ID=Msa0553050-mRNA-1:five_prime_utr;Parent=Msa0553050-mRNA-1
chr4_1 CDS 49834795 49834830 49834795 ID=Msa0553050-mRNA-1:cds;Parent=Msa0553050-mRNA-1
chr4_1 CDS 49834610 49834668 49834610 ID=Msa0553050-mRNA-1:cds;Parent=Msa0553050-mRNA-1
chr4_1 CDS 49833212 49833306 49833212 ID=Msa0553050-mRNA-1:cds;Parent=Msa0553050-mRNA-1
chr4_1 CDS 49833104 49833129 49833104 ID=Msa0553050-mRNA-1:cds;Parent=Msa0553050-mRNA-1
chr4_1 three_prime_UTR 49833013 49833103 49833013 ID=Msa0553050-mRNA-1:three_prime_utr;Parent=Msa0553050-mRNA-1
chr4_1 three_prime_UTR 49832830 49832979 49832830 ID=Msa0553050-mRNA-1:three_prime_utr;Parent=Msa0553050-mRNA-1
Gene Sequence

>Msa0553050

ATGATTATTCCCGTTCGTTGTTTCACTTGTGGAAAGGTCATTGGAAACAAATGGGACTCTTATCTGGACTTTCTTCAAGCCGATTACACTGAAGGAGATGCACTGGATGCGTTGGGTCTGGTTCGGTATTGTTGCAGGCGCATGCTTATGACCCATGTTGATCTCATTGAGAAACTATTGAACTATAACACTATGGAGAAGGCTGAAGCCAGTTAG
Protein sequence

>Msa0553050

MIIPVRCFTCGKVIGNKWDSYLDFLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTMEKAEAS*