AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059408

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059408.t1 MTR_3g107100 49.468 188 92 2 33 217 106 293 1.31e-44 155
MS.gene059408.t1 MTR_3g107120 44.240 217 107 4 9 218 93 302 6.27e-44 153
MS.gene059408.t1 MTR_3g107160 45.699 186 100 1 33 217 108 293 1.18e-40 144
MS.gene059408.t1 MTR_1g053025 42.795 229 106 9 9 219 80 301 2.71e-34 127
MS.gene059408.t1 MTR_2g007450 40.964 249 111 11 1 217 83 327 1.05e-31 121
MS.gene059408.t1 MTR_2g007400 39.912 228 121 8 2 217 80 303 1.57e-29 115
MS.gene059408.t1 MTR_4g098590 38.034 234 125 8 9 229 73 299 2.03e-29 114
MS.gene059408.t1 MTR_2g007500 35.124 242 130 6 7 224 59 297 1.44e-28 111
MS.gene059408.t1 MTR_3g107140 52.381 126 58 2 9 133 73 197 5.11e-27 104
MS.gene059408.t1 MTR_5g083890 37.712 236 123 8 1 217 73 303 1.63e-26 108
MS.gene059408.t1 MTR_2g008320 38.839 224 117 10 3 216 121 334 1.04e-25 105
MS.gene059408.t1 MTR_7g075820 35.217 230 126 6 5 217 79 302 2.47e-25 103
MS.gene059408.t1 MTR_2g008310 41.765 170 80 6 58 216 119 280 1.51e-22 95.9
MS.gene059408.t1 MTR_1g107385 42.515 167 85 6 57 217 165 326 1.62e-20 90.5
MS.gene059408.t1 MTR_2g005990 36.410 195 107 6 33 217 108 295 2.54e-20 89.7
MS.gene059408.t1 MTR_3g099830 39.024 164 90 4 57 217 117 273 2.83e-20 89.4
MS.gene059408.t1 MTR_7g007080 33.480 227 138 7 3 223 73 292 3.09e-18 84.0
MS.gene059408.t1 MTR_3g040700 33.476 233 123 8 2 223 108 319 4.52e-18 83.6
MS.gene059408.t1 MTR_3g026920 31.250 240 115 8 9 217 98 318 9.47e-18 82.4
MS.gene059408.t1 MTR_1g041245 32.018 228 140 7 1 221 75 294 1.32e-17 82.0
MS.gene059408.t1 MTR_5g058100 33.488 215 128 7 8 215 84 290 3.09e-17 80.9
MS.gene059408.t1 MTR_7g007370 34.000 200 122 5 21 217 57 249 3.71e-17 80.5
MS.gene059408.t1 MTR_7g007340 39.053 169 78 7 57 216 107 259 4.35e-17 80.5
MS.gene059408.t1 MTR_6g045333 32.895 228 131 8 3 217 133 351 1.06e-16 79.7
MS.gene059408.t1 MTR_3g108150 33.188 229 128 6 2 218 78 293 1.29e-16 79.3
MS.gene059408.t1 MTR_7g083740 34.848 198 102 8 28 217 92 270 1.97e-16 78.6
MS.gene059408.t1 MTR_5g045510 39.181 171 83 8 33 192 107 267 2.46e-16 78.2
MS.gene059408.t1 MTR_5g058000 34.101 217 126 8 8 220 83 286 3.17e-16 77.8
MS.gene059408.t1 MTR_3g036250 32.609 230 123 9 2 220 70 278 4.01e-16 77.4
MS.gene059408.t1 MTR_7g055943 32.243 214 132 6 8 215 115 321 6.26e-16 77.4
MS.gene059408.t1 MTR_7g075800 31.963 219 115 9 30 217 101 316 1.60e-15 76.6
MS.gene059408.t1 MTR_4g039440 29.911 224 144 6 1 215 96 315 5.32e-15 74.7
MS.gene059408.t1 MTR_7g032370 29.730 222 130 5 3 217 56 258 8.69e-15 73.6
MS.gene059408.t1 MTR_3g450920 39.535 129 63 4 14 137 88 206 1.16e-14 71.6
MS.gene059408.t1 MTR_5g073340 33.136 169 104 3 58 218 102 269 1.21e-14 72.8
MS.gene059408.t1 MTR_5g007410 34.343 198 111 6 33 217 124 315 2.23e-14 72.8
MS.gene059408.t1 MTR_7g034120 31.897 232 132 9 1 218 2 221 2.62e-14 72.0
MS.gene059408.t1 MTR_6g022500 30.698 215 138 6 8 217 176 384 2.85e-14 72.8
MS.gene059408.t1 MTR_6g011460 32.143 224 126 7 3 215 116 324 4.65e-14 72.0
MS.gene059408.t1 MTR_0009s0080 31.579 209 120 7 33 227 117 316 6.54e-14 71.6
MS.gene059408.t1 MTR_7g056280 32.215 149 84 2 107 254 366 498 8.18e-14 71.2
MS.gene059408.t1 MTR_3g071850 31.982 222 129 10 9 217 102 314 1.85e-13 70.5
MS.gene059408.t1 MTR_5g073420 33.333 219 124 9 3 215 82 284 2.37e-13 69.7
MS.gene059408.t1 MTR_2g036470 33.166 199 110 7 33 218 114 302 4.24e-13 68.9
MS.gene059408.t1 MTR_6g022360 32.287 223 121 9 8 215 105 312 4.68e-13 68.9
MS.gene059408.t1 MTR_5g061680 31.944 216 113 8 3 215 82 266 5.48e-13 68.2
MS.gene059408.t1 MTR_7g034090 49.367 79 34 1 146 218 147 225 1.28e-12 67.8
MS.gene059408.t1 MTR_7g034090 49.367 79 32 2 1 79 76 146 2.41e-11 63.9
MS.gene059408.t1 MTR_7g088260 29.565 230 132 6 3 218 157 370 1.47e-12 67.4
MS.gene059408.t1 MTR_7g098570 33.636 220 131 6 3 215 86 297 2.16e-12 67.0
MS.gene059408.t1 MTR_7g032380 29.302 215 110 6 9 217 84 262 2.29e-12 66.6
MS.gene059408.t1 MTR_7g033990 31.224 237 133 9 1 222 75 296 2.48e-12 66.6
MS.gene059408.t1 MTR_7g056433 37.427 171 89 9 58 217 132 295 2.48e-12 66.6
MS.gene059408.t1 MTR_3g095600 34.756 164 75 7 3 164 76 209 2.80e-12 65.9
MS.gene059408.t1 MTR_5g073370 33.146 178 110 6 45 218 115 287 2.86e-12 66.6
MS.gene059408.t1 MTR_8g085430 32.836 134 86 2 2 134 84 214 4.14e-12 65.9
MS.gene059408.t1 MTR_7g098480 34.389 221 126 8 3 215 84 293 7.96e-12 65.5
MS.gene059408.t1 MTR_7g115270 30.126 239 135 7 1 215 76 306 1.02e-11 64.7
MS.gene059408.t1 MTR_1g108800 36.646 161 84 6 61 210 93 246 1.04e-11 64.7
MS.gene059408.t1 MTR_3g106960 39.286 112 65 2 114 222 1 112 1.13e-11 63.5
MS.gene059408.t1 MTR_3g105060 31.122 196 118 5 3 192 81 265 1.14e-11 64.7
MS.gene059408.t1 MTR_1g066830 32.719 217 125 8 9 213 84 291 1.40e-11 64.3
MS.gene059408.t1 MTR_7g056417 35.762 151 81 5 58 196 7 153 2.93e-11 60.8
MS.gene059408.t1 MTR_1g059860 32.127 221 108 10 33 215 41 257 3.09e-11 63.2
MS.gene059408.t1 MTR_0492s0020 32.143 224 126 8 3 218 152 357 4.54e-11 63.2
MS.gene059408.t1 MTR_0543s0010 32.143 224 126 8 3 218 152 357 4.71e-11 63.2
MS.gene059408.t1 MTR_5g045170 34.524 168 94 6 61 217 136 298 4.72e-11 62.8
MS.gene059408.t1 MTR_1g085120 29.148 223 141 6 5 217 239 454 8.30e-11 62.4
MS.gene059408.t1 MTR_1g085120 29.148 223 141 6 5 217 164 379 8.45e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059408.t1 AT1G16930 31.111 135 87 3 13 143 88 220 4.98e-11 63.2
MS.gene059408.t1 AT1G16930 31.111 135 87 3 13 143 88 220 4.98e-11 63.2
MS.gene059408.t1 AT1G16930 31.111 135 87 3 13 143 88 220 4.98e-11 63.2

Find 68 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAGACGGAAAGTCTTAA+TGG 0.275596 6.3:+42325267 None:intergenic
CCATATGGGGATACTGTTAC+TGG 0.328519 6.3:+42325213 None:intergenic
CTTGCATGTTAGAATACTAA+TGG 0.371504 6.3:+42325114 None:intergenic
TGATAAAGAGGAGGGTTTCA+AGG 0.375494 6.3:-42323427 MS.gene059408:CDS
CCCTTCACTTGGGACGGATT+CGG 0.397357 6.3:-42325018 MS.gene059408:CDS
CCCCATATGGCTCAACTTTG+TGG 0.404307 6.3:-42325200 MS.gene059408:CDS
AAAGCTCTTCCATTCTCTAT+AGG 0.411397 6.3:+42324895 None:intergenic
AATGGGAGGTGCAGCCTCTC+TGG 0.412642 6.3:+42325285 None:intergenic
TTACTGGGATAGTGAAGTTC+GGG 0.421964 6.3:+42325229 None:intergenic
ACATAAAATCTCGAAGTTCA+TGG 0.422583 6.3:+42324993 None:intergenic
GTGAGTAAATTCTCAAGGAT+TGG 0.427217 6.3:+42324953 None:intergenic
TGGAGCTGGAATAGTGTTGC+TGG 0.441066 6.3:-42323387 MS.gene059408:CDS
GTTACTGGGATAGTGAAGTT+CGG 0.455744 6.3:+42325228 None:intergenic
TCTAAGCTTCGAATTTGATC+AGG 0.456060 6.3:-42324792 MS.gene059408:intron
TGAGTAAATTCTCAAGGATT+GGG 0.457540 6.3:+42324954 None:intergenic
ATCTCTCAAAACCCTTCACT+TGG 0.459875 6.3:-42325029 MS.gene059408:CDS
TACCACAAAGTTGAGCCATA+TGG 0.461519 6.3:+42325198 None:intergenic
ACCACAAAGTTGAGCCATAT+GGG 0.463370 6.3:+42325199 None:intergenic
TCAAAAGCTTGACCTTGATC+CGG 0.464635 6.3:-42324565 MS.gene059408:intron
AAGCGATTCCTCGGCATCAA+AGG 0.468563 6.3:+42324919 None:intergenic
TTGCATGTTAGAATACTAAT+GGG 0.472640 6.3:+42325115 None:intergenic
TTAAGACGGAAAGTCTTAAT+GGG 0.473047 6.3:+42325268 None:intergenic
TCCTTTAACAAACCGGATCA+AGG 0.483120 6.3:+42324553 None:intergenic
CCCTCCTCTTTATCACTCTC+CGG 0.486880 6.3:+42323435 None:intergenic
TGAGTCAAATTATGAAAAGT+AGG 0.488316 6.3:+42324657 None:intergenic
CCAGTAACAGTATCCCCATA+TGG 0.488901 6.3:-42325213 MS.gene059408:CDS
ACATCGTGAACTGCTTTCAA+TGG 0.490175 6.3:+42324821 None:intergenic
TAATAATTCACACACGGGAG+GGG 0.512310 6.3:-42323593 MS.gene059408:CDS
GATAAAGAGGAGGGTTTCAA+GGG 0.517389 6.3:-42323426 MS.gene059408:CDS
AGCGATTCCTCGGCATCAAA+GGG 0.521005 6.3:+42324920 None:intergenic
GATTGGGCAACCCTTTAGAA+AGG 0.523548 6.3:+42324970 None:intergenic
GGCTCAACTTTGTGGTAACA+CGG 0.523650 6.3:-42325192 MS.gene059408:CDS
TTTGAGAGATGGAAGTAGAA+CGG 0.525950 6.3:+42325042 None:intergenic
CAAGCGGATATTGATTGTGT+TGG 0.526450 6.3:-42324854 MS.gene059408:CDS
TCAAAACCCTTCACTTGGGA+CGG 0.531136 6.3:-42325024 MS.gene059408:CDS
AATCGCTTACCTATAGAGAA+TGG 0.531711 6.3:-42324904 MS.gene059408:CDS
ACCGAATCCGTCCCAAGTGA+AGG 0.539635 6.3:+42325017 None:intergenic
CTCAGCTGGAGCTTATATCT+CGG 0.548471 6.3:-42324638 MS.gene059408:CDS
TTATGCTCTAATTGCTTGCA+TGG 0.550943 6.3:-42323623 MS.gene059408:CDS
CTTAAATAACTTGATCCAAG+CGG 0.552272 6.3:-42324870 MS.gene059408:CDS
AATAATTCACACACGGGAGG+GGG 0.554772 6.3:-42323592 MS.gene059408:CDS
CTGAATGTGAAAATTGGAGC+TGG 0.556898 6.3:-42323401 MS.gene059408:CDS
TGACTTTATTAGTTTCGTGC+CGG 0.564319 6.3:-42323454 MS.gene059408:intron
CGATGTGCCCTTTGATGCCG+AGG 0.573700 6.3:-42324927 MS.gene059408:CDS
CGTGCCGGAGAGTGATAAAG+AGG 0.574292 6.3:-42323439 MS.gene059408:CDS
CAACACAATCAATATCCGCT+TGG 0.576658 6.3:+42324855 None:intergenic
CATATGGGGATACTGTTACT+GGG 0.579686 6.3:+42325214 None:intergenic
GCTCAACTTTGTGGTAACAC+GGG 0.579900 6.3:-42325191 MS.gene059408:CDS
ATAAAGAGGAGGGTTTCAAG+GGG 0.588033 6.3:-42323425 MS.gene059408:CDS
GTAATAATTCACACACGGGA+GGG 0.589277 6.3:-42323594 MS.gene059408:CDS
TCTATAGGTAAGCGATTCCT+CGG 0.589341 6.3:+42324910 None:intergenic
CGTATGTGAGTAAATTCTCA+AGG 0.603107 6.3:+42324948 None:intergenic
TATCTCTATGTGGACAGAGT+GGG 0.607530 6.3:-42325154 MS.gene059408:CDS
CCGAATCCGTCCCAAGTGAA+GGG 0.608555 6.3:+42325018 None:intergenic
AACTGTATATCAGCTTAAGA+CGG 0.612901 6.3:+42325254 None:intergenic
GCCGGAGAGTGATAAAGAGG+AGG 0.618249 6.3:-42323436 MS.gene059408:CDS
TGAATTCATATATCTCTATG+TGG 0.619775 6.3:-42325164 MS.gene059408:CDS
TGTAATAATTCACACACGGG+AGG 0.622635 6.3:-42323595 MS.gene059408:CDS
TCTCTCAAAACCCTTCACTT+GGG 0.635026 6.3:-42325028 MS.gene059408:CDS
CTCAACTTTGTGGTAACACG+GGG 0.636531 6.3:-42325190 MS.gene059408:CDS
CCGGAGAGTGATAAAGAGGA+GGG 0.637119 6.3:-42323435 MS.gene059408:CDS
CCACAAAGTTGAGCCATATG+GGG 0.646292 6.3:+42325200 None:intergenic
ATATCTCTATGTGGACAGAG+TGG 0.650784 6.3:-42325155 MS.gene059408:CDS
AATTGTAATAATTCACACAC+GGG 0.663739 6.3:-42323598 MS.gene059408:CDS
TAATTGTAATAATTCACACA+CGG 0.663810 6.3:-42323599 MS.gene059408:CDS
CTCTGTCTTGATGTCCAGAG+AGG 0.672802 6.3:-42325299 None:intergenic
AGACGGAAAGTCTTAATGGG+AGG 0.694323 6.3:+42325271 None:intergenic
TAAAGAGGAGGGTTTCAAGG+GGG 0.726966 6.3:-42323424 MS.gene059408:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAATGAAAATAAAATT+AGG + chr6.3:42324741-42324760 None:intergenic 10.0%
!! AAATAAATTAAAACACATAA+AGG + chr6.3:42324264-42324283 None:intergenic 10.0%
!! AAGACAAATTGTTAGAAAAT+AGG + chr6.3:42323957-42323976 None:intergenic 20.0%
!! AGACAAATTGTTAGAAAATA+GGG + chr6.3:42323956-42323975 None:intergenic 20.0%
!! CTAATGTAAAAGAATAAAGA+AGG + chr6.3:42324911-42324930 None:intergenic 20.0%
!! TAATTGTAATAATTCACACA+CGG - chr6.3:42325073-42325092 MS.gene059408:CDS 20.0%
!! TACTTACTATAGAATTGAAT+AGG - chr6.3:42323914-42323933 MS.gene059408:intron 20.0%
!! TCTTAAAAACCATGAAAATT+TGG - chr6.3:42324462-42324481 MS.gene059408:intron 20.0%
!!! ATTTCTCATACTGATTTTAT+AGG + chr6.3:42324204-42324223 None:intergenic 20.0%
!!! GATAATTTTCTTTCTATTTC+AGG + chr6.3:42324500-42324519 None:intergenic 20.0%
!!! TTTATTCTTTTACATTAGCA+TGG - chr6.3:42324913-42324932 MS.gene059408:CDS 20.0%
!!! TTTCTCATACTGATTTTATA+GGG + chr6.3:42324203-42324222 None:intergenic 20.0%
! AAAACCATGAAAATTTGGAA+TGG - chr6.3:42324467-42324486 MS.gene059408:intron 25.0%
! AAAATTATCCTCATTTCCAA+GGG - chr6.3:42324511-42324530 MS.gene059408:intron 25.0%
! AACATTAATACACCATGAAA+AGG + chr6.3:42324990-42325009 None:intergenic 25.0%
! AATTGTAATAATTCACACAC+GGG - chr6.3:42325074-42325093 MS.gene059408:CDS 25.0%
! CACAAAAAAAAGAGAAAGAA+AGG - chr6.3:42324849-42324868 MS.gene059408:CDS 25.0%
! GATATTATCCTTTAACAAAC+CGG + chr6.3:42324129-42324148 None:intergenic 25.0%
! TAGAGATGACAATGAAAAAT+GGG - chr6.3:42324319-42324338 MS.gene059408:intron 25.0%
! TCTAATCACAACTGAAAAAT+TGG - chr6.3:42325157-42325176 MS.gene059408:CDS 25.0%
! TGAATTCATATATCTCTATG+TGG - chr6.3:42323508-42323527 MS.gene059408:intron 25.0%
! TGAGTCAAATTATGAAAAGT+AGG + chr6.3:42324018-42324037 None:intergenic 25.0%
! TTGCATGTTAGAATACTAAT+GGG + chr6.3:42323560-42323579 None:intergenic 25.0%
!! AAAAGAATAAAGAAGGTACA+TGG + chr6.3:42324904-42324923 None:intergenic 25.0%
!! AGATTTTATGTCCTTTCTAA+AGG - chr6.3:42323691-42323710 MS.gene059408:intron 25.0%
!! ATTTTATGCATAATCATAGG+AGG - chr6.3:42324810-42324829 MS.gene059408:CDS 25.0%
!! CTGATTTTATGCATAATCAT+AGG - chr6.3:42324807-42324826 MS.gene059408:CDS 25.0%
!! GATTTTATGTCCTTTCTAAA+GGG - chr6.3:42323692-42323711 MS.gene059408:intron 25.0%
!! TTTTATGCATAATCATAGGA+GGG - chr6.3:42324811-42324830 MS.gene059408:CDS 25.0%
!! TTTTGTGCATTATTAACAGA+CGG - chr6.3:42324283-42324302 MS.gene059408:intron 25.0%
!!! CTTTCTTTCTCTTTTTTTTG+TGG + chr6.3:42324851-42324870 None:intergenic 25.0%
AACTGTATATCAGCTTAAGA+CGG + chr6.3:42323421-42323440 None:intergenic 30.0%
AATTAGAGCATAACACTGAT+AGG + chr6.3:42325042-42325061 None:intergenic 30.0%
ACATAAAATCTCGAAGTTCA+TGG + chr6.3:42323682-42323701 None:intergenic 30.0%
CACAACTGAAAAATTGGAAT+TGG - chr6.3:42325163-42325182 MS.gene059408:CDS 30.0%
CTAGAGATGACAATGAAAAA+TGG - chr6.3:42324318-42324337 MS.gene059408:intron 30.0%
CTTAAATAACTTGATCCAAG+CGG - chr6.3:42323802-42323821 MS.gene059408:intron 30.0%
CTTGCATGTTAGAATACTAA+TGG + chr6.3:42323561-42323580 None:intergenic 30.0%
GAAAATTATCCTCATTTCCA+AGG - chr6.3:42324510-42324529 MS.gene059408:intron 30.0%
GAGAATGTAGTAAGTACTAA+AGG - chr6.3:42325115-42325134 MS.gene059408:CDS 30.0%
GATGTCATTAACTTTGTCTT+TGG - chr6.3:42324667-42324686 MS.gene059408:CDS 30.0%
TAAGAAGTTGAAAAGATTGC+AGG + chr6.3:42324390-42324409 None:intergenic 30.0%
TAGAGTAACTAGTACTTACA+TGG + chr6.3:42324579-42324598 None:intergenic 30.0%
TGAGTAAATTCTCAAGGATT+GGG + chr6.3:42323721-42323740 None:intergenic 30.0%
TGTAGTAAGTACTAAAGGAT+AGG - chr6.3:42325120-42325139 MS.gene059408:CDS 30.0%
TTAAGACGGAAAGTCTTAAT+GGG + chr6.3:42323407-42323426 None:intergenic 30.0%
TTTGTCTTTGTGCATATTCT+AGG - chr6.3:42323969-42323988 MS.gene059408:intron 30.0%
! AGCAAGTACTTGATGATTTA+AGG + chr6.3:42324716-42324735 None:intergenic 30.0%
! TGCAATCTTTTCAACTTCTT+AGG - chr6.3:42324389-42324408 MS.gene059408:intron 30.0%
! TGTCTTTGGTGTATAAATTG+AGG - chr6.3:42324681-42324700 MS.gene059408:CDS 30.0%
! TTTTCATAATTTGACTCAGC+TGG - chr6.3:42324020-42324039 MS.gene059408:intron 30.0%
!! AATTAGCTTGTACCAAGTAA+TGG - chr6.3:42324615-42324634 MS.gene059408:CDS 30.0%
AAAGCTCTTCCATTCTCTAT+AGG + chr6.3:42323780-42323799 None:intergenic 35.0%
AATCGCTTACCTATAGAGAA+TGG - chr6.3:42323768-42323787 MS.gene059408:intron 35.0%
ATTGGAATTGGAACTAGAGA+TGG - chr6.3:42325175-42325194 MS.gene059408:CDS 35.0%
CAACTACTTACAAATCAACG+TGG + chr6.3:42324776-42324795 None:intergenic 35.0%
CGTATGTGAGTAAATTCTCA+AGG + chr6.3:42323727-42323746 None:intergenic 35.0%
CTTAAGACGGAAAGTCTTAA+TGG + chr6.3:42323408-42323427 None:intergenic 35.0%
GTGAGTAAATTCTCAAGGAT+TGG + chr6.3:42323722-42323741 None:intergenic 35.0%
TACCAAATCTAGCCATTACT+TGG + chr6.3:42324630-42324649 None:intergenic 35.0%
TACCAAGTAATGGCTAGATT+TGG - chr6.3:42324625-42324644 MS.gene059408:CDS 35.0%
TACTCTCACATGAAAACGAT+AGG - chr6.3:42325012-42325031 MS.gene059408:CDS 35.0%
TCTAAGCTTCGAATTTGATC+AGG - chr6.3:42323880-42323899 MS.gene059408:intron 35.0%
TGAAAAGATTGCAGGTTCTA+AGG + chr6.3:42324382-42324401 None:intergenic 35.0%
TTATGCTCTAATTGCTTGCA+TGG - chr6.3:42325049-42325068 MS.gene059408:CDS 35.0%
TTGGAATTGGAACTAGAGAT+GGG - chr6.3:42325176-42325195 MS.gene059408:CDS 35.0%
TTTGAGAGATGGAAGTAGAA+CGG + chr6.3:42323633-42323652 None:intergenic 35.0%
! ACCATGAAAATTTGGAATGG+TGG - chr6.3:42324470-42324489 MS.gene059408:intron 35.0%
! ACGCTCTTCAAATTTTCGTT+AGG - chr6.3:42324167-42324186 MS.gene059408:intron 35.0%
! TCCACCATTCCAAATTTTCA+TGG + chr6.3:42324474-42324493 None:intergenic 35.0%
! TGACTTTATTAGTTTCGTGC+CGG - chr6.3:42325218-42325237 MS.gene059408:CDS 35.0%
!! AGGTTCTAAGGTGTAATGAA+AGG + chr6.3:42324370-42324389 None:intergenic 35.0%
!!! CTTGTTGTTCTCAAGCTTTT+TGG - chr6.3:42323584-42323603 MS.gene059408:CDS 35.0%
ACACCATGAAAAGGCTGTTA+AGG + chr6.3:42324981-42325000 None:intergenic 40.0%
ACATCGTGAACTGCTTTCAA+TGG + chr6.3:42323854-42323873 None:intergenic 40.0%
ACCACAAAGTTGAGCCATAT+GGG + chr6.3:42323476-42323495 None:intergenic 40.0%
AGGCTTACAATGTGAGCTTA+TGG - chr6.3:42324409-42324428 MS.gene059408:intron 40.0%
ATAAAGAGGAGGGTTTCAAG+GGG - chr6.3:42325247-42325266 MS.gene059408:CDS 40.0%
ATATCTCTATGTGGACAGAG+TGG - chr6.3:42323517-42323536 MS.gene059408:intron 40.0%
ATCTCTCAAAACCCTTCACT+TGG - chr6.3:42323643-42323662 MS.gene059408:intron 40.0%
CAACACAATCAATATCCGCT+TGG + chr6.3:42323820-42323839 None:intergenic 40.0%
CATATGGGGATACTGTTACT+GGG + chr6.3:42323461-42323480 None:intergenic 40.0%
CATGAAAAGGCTGTTAAGGA+AGG + chr6.3:42324977-42324996 None:intergenic 40.0%
CTGAATGTGAAAATTGGAGC+TGG - chr6.3:42325271-42325290 MS.gene059408:CDS 40.0%
CTTACAATGTGAGCTTATGG+TGG - chr6.3:42324412-42324431 MS.gene059408:intron 40.0%
GATAAAGAGGAGGGTTTCAA+GGG - chr6.3:42325246-42325265 MS.gene059408:CDS 40.0%
GCATTATTAACAGACGGACA+TGG - chr6.3:42324289-42324308 MS.gene059408:intron 40.0%
GTAATAATTCACACACGGGA+GGG - chr6.3:42325078-42325097 MS.gene059408:CDS 40.0%
GTTACTGGGATAGTGAAGTT+CGG + chr6.3:42323447-42323466 None:intergenic 40.0%
TAATAATTCACACACGGGAG+GGG - chr6.3:42325079-42325098 MS.gene059408:CDS 40.0%
TACCACAAAGTTGAGCCATA+TGG + chr6.3:42323477-42323496 None:intergenic 40.0%
TATAAGCTCACACGTTGAAG+AGG + chr6.3:42324537-42324556 None:intergenic 40.0%
TATCTCTATGTGGACAGAGT+GGG - chr6.3:42323518-42323537 MS.gene059408:intron 40.0%
TCAAAAGCTTGACCTTGATC+CGG - chr6.3:42324107-42324126 MS.gene059408:intron 40.0%
TCCTTTAACAAACCGGATCA+AGG + chr6.3:42324122-42324141 None:intergenic 40.0%
TCTATAGGTAAGCGATTCCT+CGG + chr6.3:42323765-42323784 None:intergenic 40.0%
TCTCTCAAAACCCTTCACTT+GGG - chr6.3:42323644-42323663 MS.gene059408:intron 40.0%
TGATAAAGAGGAGGGTTTCA+AGG - chr6.3:42325245-42325264 MS.gene059408:CDS 40.0%
TGTAATAATTCACACACGGG+AGG - chr6.3:42325077-42325096 MS.gene059408:CDS 40.0%
TTACTGGGATAGTGAAGTTC+GGG + chr6.3:42323446-42323465 None:intergenic 40.0%
! CAAGCGGATATTGATTGTGT+TGG - chr6.3:42323818-42323837 MS.gene059408:intron 40.0%
! CTTCCTTAACAGCCTTTTCA+TGG - chr6.3:42324975-42324994 MS.gene059408:CDS 40.0%
!! ACCTTGATCCGGTTTGTTAA+AGG - chr6.3:42324118-42324137 MS.gene059408:intron 40.0%
!! AGGTGTAATGAAAGGCATTG+TGG + chr6.3:42324362-42324381 None:intergenic 40.0%
!!! AAGTGAAGGGTTTTGAGAGA+TGG + chr6.3:42323644-42323663 None:intergenic 40.0%
!!! AGGTCAAGCTTTTGAAGCTT+AGG + chr6.3:42324102-42324121 None:intergenic 40.0%
!!! CAAGCTTTTTGGTTTCTGTG+TGG - chr6.3:42323595-42323614 MS.gene059408:CDS 40.0%
!!! CTTTTTGGTTTCTGTGTGGA+GGG - chr6.3:42323599-42323618 MS.gene059408:CDS 40.0%
!!! GGTCAAGCTTTTGAAGCTTA+GGG + chr6.3:42324101-42324120 None:intergenic 40.0%
AATAATTCACACACGGGAGG+GGG - chr6.3:42325080-42325099 MS.gene059408:CDS 45.0%
AGACGGAAAGTCTTAATGGG+AGG + chr6.3:42323404-42323423 None:intergenic 45.0%
AGGGGGCTGAATGTGAAAAT+TGG - chr6.3:42325265-42325284 MS.gene059408:CDS 45.0%
ATTAACAGACGGACATGGCA+TGG - chr6.3:42324294-42324313 MS.gene059408:intron 45.0%
CCACAAAGTTGAGCCATATG+GGG + chr6.3:42323475-42323494 None:intergenic 45.0%
CCAGTAACAGTATCCCCATA+TGG - chr6.3:42323459-42323478 MS.gene059408:intron 45.0%
CCATATGGGGATACTGTTAC+TGG + chr6.3:42323462-42323481 None:intergenic 45.0%
CTCAACTTTGTGGTAACACG+GGG - chr6.3:42323482-42323501 MS.gene059408:intron 45.0%
CTCAGCTGGAGCTTATATCT+CGG - chr6.3:42324034-42324053 MS.gene059408:intron 45.0%
GAAAAATGGGTAGAGCCAGA+TGG - chr6.3:42324332-42324351 MS.gene059408:intron 45.0%
GCTCAACTTTGTGGTAACAC+GGG - chr6.3:42323481-42323500 MS.gene059408:intron 45.0%
GGCTCAACTTTGTGGTAACA+CGG - chr6.3:42323480-42323499 MS.gene059408:intron 45.0%
GTGTGTTAAAAGTCCCACAC+TGG + chr6.3:42324240-42324259 None:intergenic 45.0%
TAAAGAGGAGGGTTTCAAGG+GGG - chr6.3:42325248-42325267 MS.gene059408:CDS 45.0%
TCAAAACCCTTCACTTGGGA+CGG - chr6.3:42323648-42323667 MS.gene059408:intron 45.0%
! GATTGGGCAACCCTTTAGAA+AGG + chr6.3:42323705-42323724 None:intergenic 45.0%
!!! GCTTTTTGGTTTCTGTGTGG+AGG - chr6.3:42323598-42323617 MS.gene059408:CDS 45.0%
AAGCGATTCCTCGGCATCAA+AGG + chr6.3:42323756-42323775 None:intergenic 50.0%
AGCGATTCCTCGGCATCAAA+GGG + chr6.3:42323755-42323774 None:intergenic 50.0%
CCCCATATGGCTCAACTTTG+TGG - chr6.3:42323472-42323491 MS.gene059408:intron 50.0%
CCCTCCTCTTTATCACTCTC+CGG + chr6.3:42325240-42325259 None:intergenic 50.0%
CCGGAGAGTGATAAAGAGGA+GGG - chr6.3:42325237-42325256 MS.gene059408:CDS 50.0%
TGAAGAGGCCCTTGGAAATG+AGG + chr6.3:42324522-42324541 None:intergenic 50.0%
TGGAGCTGGAATAGTGTTGC+TGG - chr6.3:42325285-42325304 MS.gene059408:CDS 50.0%
TTCGCAGTCTCATCCAGTGT+GGG - chr6.3:42324224-42324243 MS.gene059408:intron 50.0%
TTTCGCAGTCTCATCCAGTG+TGG - chr6.3:42324223-42324242 MS.gene059408:intron 50.0%
! AATGTGAGCTTATGGTGGCG+AGG - chr6.3:42324417-42324436 MS.gene059408:intron 50.0%
! AGGCATTGTGGAACACCATC+TGG + chr6.3:42324350-42324369 None:intergenic 50.0%
!!! GCTTTTGAAGCTTAGGGCAG+CGG + chr6.3:42324095-42324114 None:intergenic 50.0%
ACCGAATCCGTCCCAAGTGA+AGG + chr6.3:42323658-42323677 None:intergenic 55.0%
CCGAATCCGTCCCAAGTGAA+GGG + chr6.3:42323657-42323676 None:intergenic 55.0%
CGTGCCGGAGAGTGATAAAG+AGG - chr6.3:42325233-42325252 MS.gene059408:CDS 55.0%
GCCGGAGAGTGATAAAGAGG+AGG - chr6.3:42325236-42325255 MS.gene059408:CDS 55.0%
TCACACGTTGAAGAGGCCCT+TGG + chr6.3:42324530-42324549 None:intergenic 55.0%
! CCCTTCACTTGGGACGGATT+CGG - chr6.3:42323654-42323673 MS.gene059408:intron 55.0%
CGATGTGCCCTTTGATGCCG+AGG - chr6.3:42323745-42323764 MS.gene059408:intron 60.0%
! AATGGGAGGTGCAGCCTCTC+TGG + chr6.3:42323390-42323409 None:intergenic 60.0%
CACACACGGGAGGGGGACAT+GGG - chr6.3:42325087-42325106 MS.gene059408:CDS 65.0%
TCACACACGGGAGGGGGACA+TGG - chr6.3:42325086-42325105 MS.gene059408:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 42323383 42325311 42323383 ID=MS.gene059408
chr6.3 mRNA 42323383 42325311 42323383 ID=MS.gene059408.t1;Parent=MS.gene059408
chr6.3 exon 42324793 42325311 42324793 ID=MS.gene059408.t1.exon1;Parent=MS.gene059408.t1
chr6.3 CDS 42324793 42325311 42324793 ID=cds.MS.gene059408.t1;Parent=MS.gene059408.t1
chr6.3 exon 42324566 42324703 42324566 ID=MS.gene059408.t1.exon2;Parent=MS.gene059408.t1
chr6.3 CDS 42324566 42324703 42324566 ID=cds.MS.gene059408.t1;Parent=MS.gene059408.t1
chr6.3 exon 42323571 42323647 42323571 ID=MS.gene059408.t1.exon3;Parent=MS.gene059408.t1
chr6.3 CDS 42323571 42323647 42323571 ID=cds.MS.gene059408.t1;Parent=MS.gene059408.t1
chr6.3 exon 42323383 42323464 42323383 ID=MS.gene059408.t1.exon4;Parent=MS.gene059408.t1
chr6.3 CDS 42323383 42323464 42323383 ID=cds.MS.gene059408.t1;Parent=MS.gene059408.t1
Gene Sequence

>MS.gene059408

ATGTCCAGAGAGGCTGCACCTCCCATTAAGACTTTCCGTCTTAAGCTGATATACAGTTATCCCGAACTTCACTATCCCAGTAACAGTATCCCCATATGGCTCAACTTTGTGGTAACACGGGGTGTTGAATTCATATATCTCTATGTGGACAGAGTGGGTTGTTTACAAAAATTACCCATTAGTATTCTAACATGCAAGACACTTGTTGTTCTCAAGCTTTTTGGTTTCTGTGTGGAGGGTTTTTCTTCCGTTCTACTTCCATCTCTCAAAACCCTTCACTTGGGACGGATTCGGTTCCATGAACTTCGAGATTTTATGTCCTTTCTAAAGGGTTGCCCAATCCTTGAGAATTTACTCACATACGATGTGCCCTTTGATGCCGAGGAATCGCTTACCTATAGAGAATGGAAGAGCTTTTGCTTAAATAACTTGATCCAAGCGGATATTGATTGTGTTGGTTCTTACTTTCCATTGAAAGCAGTTCACGATGTACTGTCTCTAAGCTTCGAATTTGATCAGGTCTATTAATGATACTTACTATAGAATTGAATAGGAAAGAATATACGTTTGCCCTATTTTCTAACAATTTGTCTTTGTGCATATTCTAGGATCATTTCTGCAATGATTTTCTTCCTACTTTTCATAATTTGACTCAGCTGGAGCTTATATCTCGGAATTTTAGTTTGAAATTCTTAATAGAAGTTCTCAACCGCTGCCCTAAGCTTCAAAAGCTTGACCTTGATCCGGTTTGTTAAAGGATAATATCTGATTTATTTATTCATTCACGCTCTTCAAATTTTCGTTAGGCTTAACTCAACCCTATAAAATCAGTATGAGAAATTTCGCAGTCTCATCCAGTGTGGGACTTTTAACACACACCTTTATGTGTTTTAATTTATTTTTTGTGCATTATTAACAGACGGACATGGCATGGACTAGAGATGACAATGAAAAATGGGTAGAGCCAGATGGTGTTCCACAATGCCTTTCATTACACCTTAGAACCTGCAATCTTTTCAACTTCTTAGGCTTACAATGTGAGCTTATGGTGGCGAGGTATATTTTGAAGAATGCAATAGTCTTAAAAACCATGAAAATTTGGAATGGTGGACAACCTGAAATAGAAAGAAAATTATCCTCATTTCCAAGGGCCTCTTCAACGTGTGAGCTTATAGTTTATGATGATTCATGATCCATGTAAGTACTAGTTACTCTATCATTTTTAATTAGCTAATTAGCTTGTACCAAGTAATGGCTAGATTTGGTAGTTTGAACTAGAACTTTGATGTCATTAACTTTGTCTTTGGTGTATAAATTGAGGAAATGTATTCCTTAAATCATCAAGTACTTGCTTACCTAATTTTATTTTCATTTGTTTATGTGTTCATATCCACGTTGATTTGTAAGTAGTTGTATGCTAAATTCTGATTTTATGCATAATCATAGGAGGGAGAGTGCAATTGTTCCACAAAAAAAAGAGAAAGAAAGGTTCTATTATAAATTAGTAGATCATTTTTGCCATGTACCTTCTTTATTCTTTTACATTAGCATGGACAAAGATTAAACTAAGTAGTTGTATTCTCAAATGATTCCTTCCTTAACAGCCTTTTCATGGTGTATTAATGTTTTTACTCTCACATGAAAACGATAGGTTTTCCTATCAGTGTTATGCTCTAATTGCTTGCATGGCTAATTGTAATAATTCACACACGGGAGGGGGACATGGGTTGCTGAGAATGTAGTAAGTACTAAAGGATAGGTTTGAGAAGTTATTTCTAATCACAACTGAAAAATTGGAATTGGAACTAGAGATGGGAACTTTTTCATTGTGAGAATGACTTTATTAGTTTCGTGCCGGAGAGTGATAAAGAGGAGGGTTTCAAGGGGGCTGAATGTGAAAATTGGAGCTGGAATAGTGTTGCTGGATGA
Protein sequence

>MS.gene059408.t1

MSREAAPPIKTFRLKLIYSYPELHYPSNSIPIWLNFVVTRGVEFIYLYVDRVGCLQKLPISILTCKTLVVLKLFGFCVEGFSSVLLPSLKTLHLGRIRFHELRDFMSFLKGCPILENLLTYDVPFDAEESLTYREWKSFCLNNLIQADIDCVGSYFPLKAVHDVLSLSFEFDQDHFCNDFLPTFHNLTQLELISRNFSLKFLIEVLNRCPKLQKLDLDPCYALIACMANCNNSHTGGGHGLLRMYFVPESDKEEGFKGAECENWSWNSVAG