Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03755.t1 | XP_013455878.1 | 89.8 | 246 | 25 | 0 | 1 | 246 | 219 | 464 | 7.40E-118 | 433.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03755.t1 | A0A072UJK5 | 89.8 | 246 | 25 | 0 | 1 | 246 | 219 | 464 | 5.3e-118 | 433.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03755.t1 | MTR_4g056030 | 89.837 | 246 | 25 | 0 | 1 | 246 | 219 | 464 | 2.94e-156 | 442 |
| MS.gene03755.t1 | MTR_4g056020 | 84.052 | 232 | 37 | 0 | 1 | 232 | 219 | 450 | 7.96e-137 | 392 |
| MS.gene03755.t1 | MTR_4g055990 | 81.013 | 237 | 40 | 1 | 1 | 232 | 256 | 492 | 3.54e-131 | 380 |
| MS.gene03755.t1 | MTR_4g055580 | 86.638 | 232 | 30 | 1 | 1 | 232 | 347 | 577 | 2.17e-127 | 373 |
| MS.gene03755.t1 | MTR_4g055990 | 80.804 | 224 | 38 | 1 | 1 | 219 | 256 | 479 | 1.63e-122 | 358 |
| MS.gene03755.t1 | MTR_4g056040 | 80.365 | 219 | 43 | 0 | 1 | 219 | 249 | 467 | 1.41e-120 | 353 |
| MS.gene03755.t1 | MTR_1g038710 | 69.912 | 226 | 64 | 2 | 1 | 224 | 233 | 456 | 5.85e-103 | 306 |
| MS.gene03755.t1 | MTR_4g056070 | 79.888 | 179 | 36 | 0 | 1 | 179 | 259 | 437 | 1.27e-97 | 292 |
| MS.gene03755.t1 | MTR_7g110920 | 62.185 | 238 | 68 | 4 | 1 | 219 | 86 | 320 | 2.36e-93 | 277 |
| MS.gene03755.t1 | MTR_4g056020 | 87.417 | 151 | 19 | 0 | 79 | 229 | 1 | 151 | 2.59e-93 | 271 |
| MS.gene03755.t1 | MTR_1g090883 | 67.164 | 134 | 44 | 0 | 1 | 134 | 229 | 362 | 1.62e-54 | 179 |
| MS.gene03755.t1 | MTR_1g090890 | 61.538 | 91 | 29 | 2 | 132 | 219 | 29 | 116 | 1.93e-31 | 112 |
| MS.gene03755.t1 | MTR_6g029350 | 28.182 | 220 | 129 | 8 | 15 | 227 | 6 | 203 | 1.14e-13 | 68.6 |
| MS.gene03755.t1 | MTR_5g008950 | 32.075 | 106 | 67 | 2 | 93 | 195 | 233 | 336 | 5.01e-12 | 65.1 |
| MS.gene03755.t1 | MTR_7g056433 | 26.866 | 134 | 88 | 4 | 88 | 215 | 253 | 382 | 6.25e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGACTTAAATTTATTTAT+TGG | 0.117769 | 4.3:-45125760 | None:intergenic |
| TCACAATTTATTATCTTTAT+CGG | 0.257922 | 4.3:+45124672 | MS.gene03755:CDS |
| AGAAATCCTCCGTACTAGTA+TGG | 0.259970 | 4.3:+45125309 | MS.gene03755:CDS |
| ATGACTTAAATTTATTTATT+GGG | 0.271672 | 4.3:-45125759 | None:intergenic |
| CTCATTACATTTGAATTTGT+TGG | 0.272022 | 4.3:+45125152 | MS.gene03755:CDS |
| ATTGCAAGATCCTTAAGTTT+TGG | 0.305825 | 4.3:-45124751 | None:intergenic |
| AGTTTCCAACCTCCTTTCTT+CGG | 0.314970 | 4.3:-45125082 | None:intergenic |
| TAATTCAATTGAAGATCATT+TGG | 0.321337 | 4.3:+45125692 | MS.gene03755:CDS |
| ATCTTTGTTATGCAGTTTCT+CGG | 0.334332 | 4.3:+45124610 | MS.gene03755:intron |
| GTTTCCAACCTCCTTTCTTC+GGG | 0.346723 | 4.3:-45125081 | None:intergenic |
| GAGATCTTCAATACCCTTGA+AGG | 0.396272 | 4.3:-45125718 | None:intergenic |
| ACTTTCTAATGTCAAATCAT+TGG | 0.400805 | 4.3:+45124483 | MS.gene03755:CDS |
| TGACATTAGAAAGTCCCTTC+AGG | 0.408452 | 4.3:-45124474 | None:intergenic |
| AATAGCAGCTTCATCAAGAT+TGG | 0.411607 | 4.3:-45124410 | None:intergenic |
| ACTTAAGGATCTTGCAATTG+AGG | 0.413085 | 4.3:+45124756 | MS.gene03755:CDS |
| ACACAATGCACCAAAACTTA+AGG | 0.445433 | 4.3:+45124741 | MS.gene03755:CDS |
| TACAAATTGTTAAAATCCGC+AGG | 0.464746 | 4.3:-45124445 | None:intergenic |
| GAGAAAGATCTTCAAGTGAC+GGG | 0.472802 | 4.3:-45124339 | None:intergenic |
| TGAAATCAATTCCCGAAGAA+AGG | 0.482837 | 4.3:+45125070 | MS.gene03755:CDS |
| AGATCATTTGGATGCCTTCA+AGG | 0.486402 | 4.3:+45125704 | MS.gene03755:CDS |
| GGAGGTTGGAAACTCGAGTT+GGG | 0.509671 | 4.3:+45125091 | MS.gene03755:CDS |
| TGTTATGCAGTTTCTCGGCA+TGG | 0.521848 | 4.3:+45124615 | MS.gene03755:intron |
| TAACACTTAATAACAGAAGT+AGG | 0.522805 | 4.3:-45124298 | None:intergenic |
| GATCATTTGGATGCCTTCAA+GGG | 0.544625 | 4.3:+45125705 | MS.gene03755:CDS |
| GGTGGAGCCATTGATATCTC+CGG | 0.546718 | 4.3:+45125112 | MS.gene03755:CDS |
| AAATTCAAATGTAATGAGAC+TGG | 0.547394 | 4.3:-45125147 | None:intergenic |
| GAAATCCTCCGTACTAGTAT+GGG | 0.549114 | 4.3:+45125310 | MS.gene03755:CDS |
| GACGGGGCATTAATTTGTAG+TGG | 0.550813 | 4.3:-45124322 | None:intergenic |
| CAATTTGTATACAGTCCTGA+AGG | 0.553354 | 4.3:+45124459 | MS.gene03755:CDS |
| AGGAGGTTGGAAACTCGAGT+TGG | 0.571978 | 4.3:+45125090 | MS.gene03755:CDS |
| AACTCGTACCCATACTAGTA+CGG | 0.577624 | 4.3:-45125318 | None:intergenic |
| AGACTGGTTGTAAGACATGC+CGG | 0.580136 | 4.3:-45125131 | None:intergenic |
| AGAGAAAGATCTTCAAGTGA+CGG | 0.585255 | 4.3:-45124340 | None:intergenic |
| AAATATCTGAGAATTGCAGT+TGG | 0.586019 | 4.3:+45124698 | MS.gene03755:CDS |
| TCACTCAAGTAAGCTAGAGA+AGG | 0.587930 | 4.3:+45125223 | MS.gene03755:CDS |
| AGACATGCCGGAGATATCAA+TGG | 0.591239 | 4.3:-45125119 | None:intergenic |
| AATTCCCGAAGAAAGGAGGT+TGG | 0.591474 | 4.3:+45125077 | MS.gene03755:CDS |
| AATCAATTCCCGAAGAAAGG+AGG | 0.619715 | 4.3:+45125073 | MS.gene03755:CDS |
| GGTTGGAAACTCGAGTTGGG+TGG | 0.621687 | 4.3:+45125094 | MS.gene03755:CDS |
| AATTTGTATACAGTCCTGAA+GGG | 0.640048 | 4.3:+45124460 | MS.gene03755:CDS |
| TCGTACCCATACTAGTACGG+AGG | 0.652397 | 4.3:-45125315 | None:intergenic |
| AGAAAGATCTTCAAGTGACG+GGG | 0.690406 | 4.3:-45124338 | None:intergenic |
| TATGCAGTTTCTCGGCATGG+AGG | 0.704407 | 4.3:+45124618 | MS.gene03755:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATATTAATTTTTTTAT+CGG | + | chr4.3:45125598-45125617 | MS.gene03755:intron | 0.0% |
| !! | TAAAATTAAGTATTCAATAT+TGG | + | chr4.3:45125658-45125677 | MS.gene03755:intron | 10.0% |
| !!! | AAAATTCTAATGATAATTTT+TGG | + | chr4.3:45125028-45125047 | MS.gene03755:intron | 10.0% |
| !! | ATGAAACTTGTAATTAAAAA+AGG | - | chr4.3:45124552-45124571 | None:intergenic | 15.0% |
| !! | TCACAATTTATTATCTTTAT+CGG | + | chr4.3:45124672-45124691 | MS.gene03755:CDS | 15.0% |
| !!! | TTGCACAATTTTTTTTTTAA+AGG | + | chr4.3:45124802-45124821 | MS.gene03755:intron | 15.0% |
| !! | AAGTAAAATAGTTTGAAAGA+AGG | - | chr4.3:45124980-45124999 | None:intergenic | 20.0% |
| !! | TAATTCAATTGAAGATCATT+TGG | + | chr4.3:45125692-45125711 | MS.gene03755:CDS | 20.0% |
| !! | TATATATACTATTACATTCG+TGG | + | chr4.3:45125519-45125538 | MS.gene03755:intron | 20.0% |
| !! | TTATCTTATATGCTATCATT+TGG | + | chr4.3:45125005-45125024 | MS.gene03755:intron | 20.0% |
| !!! | ACTTTCTGTTTTAATGAAAA+AGG | + | chr4.3:45125377-45125396 | MS.gene03755:intron | 20.0% |
| !!! | TTGGTATGTTAATATTTTGT+TGG | + | chr4.3:45125400-45125419 | MS.gene03755:intron | 20.0% |
| !!! | TTTGTAAAGTCAAATTTTCT+AGG | - | chr4.3:45124385-45124404 | None:intergenic | 20.0% |
| ! | AAATTCAAATGTAATGAGAC+TGG | - | chr4.3:45125150-45125169 | None:intergenic | 25.0% |
| ! | AATATAATAAGCTCAAAACC+TGG | + | chr4.3:45124829-45124848 | MS.gene03755:intron | 25.0% |
| ! | ACTTTCTAATGTCAAATCAT+TGG | + | chr4.3:45124483-45124502 | MS.gene03755:CDS | 25.0% |
| ! | AGTTTGAAAGAAGGTTAAAT+TGG | - | chr4.3:45124971-45124990 | None:intergenic | 25.0% |
| ! | CTCATTACATTTGAATTTGT+TGG | + | chr4.3:45125152-45125171 | MS.gene03755:CDS | 25.0% |
| ! | TAACACTTAATAACAGAAGT+AGG | - | chr4.3:45124301-45124320 | None:intergenic | 25.0% |
| ! | TCAAAACATAAAAACTGAGT+TGG | + | chr4.3:45125178-45125197 | MS.gene03755:CDS | 25.0% |
| !! | AAGTATTCAATATTGGTTAC+AGG | + | chr4.3:45125665-45125684 | MS.gene03755:intron | 25.0% |
| !!! | TCTGTTTTAATGAAAAAGGT+TGG | + | chr4.3:45125381-45125400 | MS.gene03755:intron | 25.0% |
| !!! | TTCTCTTGTTGATTTTTCAT+TGG | + | chr4.3:45124357-45124376 | MS.gene03755:CDS | 25.0% |
| AAATATCTGAGAATTGCAGT+TGG | + | chr4.3:45124698-45124717 | MS.gene03755:CDS | 30.0% | |
| AATGTCTGTAAGAGTAAGAA+AGG | - | chr4.3:45125459-45125478 | None:intergenic | 30.0% | |
| AATTTGTATACAGTCCTGAA+GGG | + | chr4.3:45124460-45124479 | MS.gene03755:CDS | 30.0% | |
| AGTAGAAAAAAGTTTACGCA+AGG | + | chr4.3:45125280-45125299 | MS.gene03755:CDS | 30.0% | |
| GAGATATAAAGTTGTGTTGA+TGG | - | chr4.3:45125490-45125509 | None:intergenic | 30.0% | |
| GTAGAAAAAAGTTTACGCAA+GGG | + | chr4.3:45125281-45125300 | MS.gene03755:CDS | 30.0% | |
| TACAAATTGTTAAAATCCGC+AGG | - | chr4.3:45124448-45124467 | None:intergenic | 30.0% | |
| TAGAAAAAAGTTTACGCAAG+GGG | + | chr4.3:45125282-45125301 | MS.gene03755:CDS | 30.0% | |
| TGAAAGAAGGTTAAATTGGA+AGG | - | chr4.3:45124967-45124986 | None:intergenic | 30.0% | |
| ! | AACTATACGTATCTTTTGCT+AGG | - | chr4.3:45124872-45124891 | None:intergenic | 30.0% |
| ! | ATCTTTGTTATGCAGTTTCT+CGG | + | chr4.3:45124610-45124629 | MS.gene03755:intron | 30.0% |
| ! | ATTGCAAGATCCTTAAGTTT+TGG | - | chr4.3:45124754-45124773 | None:intergenic | 30.0% |
| ! | ATTTTGTTGGTATGATGTTG+TGG | + | chr4.3:45125413-45125432 | MS.gene03755:intron | 30.0% |
| ! | GAAAGTATATAGACTCTGTA+TGG | - | chr4.3:45125363-45125382 | None:intergenic | 30.0% |
| ! | TATTTTCATTTGTAAGCCTG+CGG | + | chr4.3:45124429-45124448 | MS.gene03755:CDS | 30.0% |
| ! | TTTTGTTGGTATGATGTTGT+GGG | + | chr4.3:45125414-45125433 | MS.gene03755:intron | 30.0% |
| !! | TACTCTTCTTTTCTTTGAAG+TGG | - | chr4.3:45125264-45125283 | None:intergenic | 30.0% |
| !! | TATAAAGTTGTGTTGATGGT+CGG | - | chr4.3:45125486-45125505 | None:intergenic | 30.0% |
| !!! | ACTCTTCTTTTCTTTGAAGT+GGG | - | chr4.3:45125263-45125282 | None:intergenic | 30.0% |
| AATAGCAGCTTCATCAAGAT+TGG | - | chr4.3:45124413-45124432 | None:intergenic | 35.0% | |
| ACACAATGCACCAAAACTTA+AGG | + | chr4.3:45124741-45124760 | MS.gene03755:CDS | 35.0% | |
| ACTTAAGGATCTTGCAATTG+AGG | + | chr4.3:45124756-45124775 | MS.gene03755:CDS | 35.0% | |
| AGAGAAAGATCTTCAAGTGA+CGG | - | chr4.3:45124343-45124362 | None:intergenic | 35.0% | |
| CAATTTGTATACAGTCCTGA+AGG | + | chr4.3:45124459-45124478 | MS.gene03755:CDS | 35.0% | |
| TGAAATCAATTCCCGAAGAA+AGG | + | chr4.3:45125070-45125089 | MS.gene03755:CDS | 35.0% | |
| TTTGTTGGTATGATGTTGTG+GGG | + | chr4.3:45125415-45125434 | MS.gene03755:intron | 35.0% | |
| AACTCGTACCCATACTAGTA+CGG | - | chr4.3:45125321-45125340 | None:intergenic | 40.0% | |
| AATCAATTCCCGAAGAAAGG+AGG | + | chr4.3:45125073-45125092 | MS.gene03755:CDS | 40.0% | |
| AGAAAGATCTTCAAGTGACG+GGG | - | chr4.3:45124341-45124360 | None:intergenic | 40.0% | |
| AGAAATCCTCCGTACTAGTA+TGG | + | chr4.3:45125309-45125328 | MS.gene03755:CDS | 40.0% | |
| AGATCATTTGGATGCCTTCA+AGG | + | chr4.3:45125704-45125723 | MS.gene03755:CDS | 40.0% | |
| AGTTTCCAACCTCCTTTCTT+CGG | - | chr4.3:45125085-45125104 | None:intergenic | 40.0% | |
| GAAATCCTCCGTACTAGTAT+GGG | + | chr4.3:45125310-45125329 | MS.gene03755:CDS | 40.0% | |
| GAGAAAGATCTTCAAGTGAC+GGG | - | chr4.3:45124342-45124361 | None:intergenic | 40.0% | |
| GAGATCTTCAATACCCTTGA+AGG | - | chr4.3:45125721-45125740 | None:intergenic | 40.0% | |
| GATCATTTGGATGCCTTCAA+GGG | + | chr4.3:45125705-45125724 | MS.gene03755:CDS | 40.0% | |
| TCACTCAAGTAAGCTAGAGA+AGG | + | chr4.3:45125223-45125242 | MS.gene03755:CDS | 40.0% | |
| TGACATTAGAAAGTCCCTTC+AGG | - | chr4.3:45124477-45124496 | None:intergenic | 40.0% | |
| AATTCCCGAAGAAAGGAGGT+TGG | + | chr4.3:45125077-45125096 | MS.gene03755:CDS | 45.0% | |
| AGACATGCCGGAGATATCAA+TGG | - | chr4.3:45125122-45125141 | None:intergenic | 45.0% | |
| GTTTCCAACCTCCTTTCTTC+GGG | - | chr4.3:45125084-45125103 | None:intergenic | 45.0% | |
| TGTTATGCAGTTTCTCGGCA+TGG | + | chr4.3:45124615-45124634 | MS.gene03755:intron | 45.0% | |
| ! | AGACTGGTTGTAAGACATGC+CGG | - | chr4.3:45125134-45125153 | None:intergenic | 45.0% |
| !! | GACGGGGCATTAATTTGTAG+TGG | - | chr4.3:45124325-45124344 | None:intergenic | 45.0% |
| !!! | ATATATTTAAAAATTTTAAG+AGG | - | chr4.3:45125586-45125605 | None:intergenic | 5.0% |
| AGGAGGTTGGAAACTCGAGT+TGG | + | chr4.3:45125090-45125109 | MS.gene03755:CDS | 50.0% | |
| GGAGGTTGGAAACTCGAGTT+GGG | + | chr4.3:45125091-45125110 | MS.gene03755:CDS | 50.0% | |
| GGTGGAGCCATTGATATCTC+CGG | + | chr4.3:45125112-45125131 | MS.gene03755:CDS | 50.0% | |
| GTCTTTGCTCTTCTTGTGCC+AGG | - | chr4.3:45124850-45124869 | None:intergenic | 50.0% | |
| GTTGGTATGATGTTGTGGGG+TGG | + | chr4.3:45125418-45125437 | MS.gene03755:intron | 50.0% | |
| TATGCAGTTTCTCGGCATGG+AGG | + | chr4.3:45124618-45124637 | MS.gene03755:intron | 50.0% | |
| TCGTACCCATACTAGTACGG+AGG | - | chr4.3:45125318-45125337 | None:intergenic | 50.0% | |
| !! | GGTTGGAAACTCGAGTTGGG+TGG | + | chr4.3:45125094-45125113 | MS.gene03755:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 45124295 | 45125770 | 45124295 | ID=MS.gene03755 |
| chr4.3 | mRNA | 45124295 | 45125770 | 45124295 | ID=MS.gene03755.t1;Parent=MS.gene03755 |
| chr4.3 | exon | 45124295 | 45124528 | 45124295 | ID=MS.gene03755.t1.exon1;Parent=MS.gene03755.t1 |
| chr4.3 | CDS | 45124295 | 45124528 | 45124295 | ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1 |
| chr4.3 | exon | 45124625 | 45124777 | 45124625 | ID=MS.gene03755.t1.exon2;Parent=MS.gene03755.t1 |
| chr4.3 | CDS | 45124625 | 45124777 | 45124625 | ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1 |
| chr4.3 | exon | 45125062 | 45125331 | 45125062 | ID=MS.gene03755.t1.exon3;Parent=MS.gene03755.t1 |
| chr4.3 | CDS | 45125062 | 45125331 | 45125062 | ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1 |
| chr4.3 | exon | 45125687 | 45125770 | 45125687 | ID=MS.gene03755.t1.exon4;Parent=MS.gene03755.t1 |
| chr4.3 | CDS | 45125687 | 45125770 | 45125687 | ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1 |
| Gene Sequence |
| Protein sequence |