AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03755


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03755.t1 MTR_4g056030 89.837 246 25 0 1 246 219 464 2.94e-156 442
MS.gene03755.t1 MTR_4g056020 84.052 232 37 0 1 232 219 450 7.96e-137 392
MS.gene03755.t1 MTR_4g055990 81.013 237 40 1 1 232 256 492 3.54e-131 380
MS.gene03755.t1 MTR_4g055580 86.638 232 30 1 1 232 347 577 2.17e-127 373
MS.gene03755.t1 MTR_4g055990 80.804 224 38 1 1 219 256 479 1.63e-122 358
MS.gene03755.t1 MTR_4g056040 80.365 219 43 0 1 219 249 467 1.41e-120 353
MS.gene03755.t1 MTR_1g038710 69.912 226 64 2 1 224 233 456 5.85e-103 306
MS.gene03755.t1 MTR_4g056070 79.888 179 36 0 1 179 259 437 1.27e-97 292
MS.gene03755.t1 MTR_7g110920 62.185 238 68 4 1 219 86 320 2.36e-93 277
MS.gene03755.t1 MTR_4g056020 87.417 151 19 0 79 229 1 151 2.59e-93 271
MS.gene03755.t1 MTR_1g090883 67.164 134 44 0 1 134 229 362 1.62e-54 179
MS.gene03755.t1 MTR_1g090890 61.538 91 29 2 132 219 29 116 1.93e-31 112
MS.gene03755.t1 MTR_6g029350 28.182 220 129 8 15 227 6 203 1.14e-13 68.6
MS.gene03755.t1 MTR_5g008950 32.075 106 67 2 93 195 233 336 5.01e-12 65.1
MS.gene03755.t1 MTR_7g056433 26.866 134 88 4 88 215 253 382 6.25e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGACTTAAATTTATTTAT+TGG 0.117769 4.3:-45125760 None:intergenic
TCACAATTTATTATCTTTAT+CGG 0.257922 4.3:+45124672 MS.gene03755:CDS
AGAAATCCTCCGTACTAGTA+TGG 0.259970 4.3:+45125309 MS.gene03755:CDS
ATGACTTAAATTTATTTATT+GGG 0.271672 4.3:-45125759 None:intergenic
CTCATTACATTTGAATTTGT+TGG 0.272022 4.3:+45125152 MS.gene03755:CDS
ATTGCAAGATCCTTAAGTTT+TGG 0.305825 4.3:-45124751 None:intergenic
AGTTTCCAACCTCCTTTCTT+CGG 0.314970 4.3:-45125082 None:intergenic
TAATTCAATTGAAGATCATT+TGG 0.321337 4.3:+45125692 MS.gene03755:CDS
ATCTTTGTTATGCAGTTTCT+CGG 0.334332 4.3:+45124610 MS.gene03755:intron
GTTTCCAACCTCCTTTCTTC+GGG 0.346723 4.3:-45125081 None:intergenic
GAGATCTTCAATACCCTTGA+AGG 0.396272 4.3:-45125718 None:intergenic
ACTTTCTAATGTCAAATCAT+TGG 0.400805 4.3:+45124483 MS.gene03755:CDS
TGACATTAGAAAGTCCCTTC+AGG 0.408452 4.3:-45124474 None:intergenic
AATAGCAGCTTCATCAAGAT+TGG 0.411607 4.3:-45124410 None:intergenic
ACTTAAGGATCTTGCAATTG+AGG 0.413085 4.3:+45124756 MS.gene03755:CDS
ACACAATGCACCAAAACTTA+AGG 0.445433 4.3:+45124741 MS.gene03755:CDS
TACAAATTGTTAAAATCCGC+AGG 0.464746 4.3:-45124445 None:intergenic
GAGAAAGATCTTCAAGTGAC+GGG 0.472802 4.3:-45124339 None:intergenic
TGAAATCAATTCCCGAAGAA+AGG 0.482837 4.3:+45125070 MS.gene03755:CDS
AGATCATTTGGATGCCTTCA+AGG 0.486402 4.3:+45125704 MS.gene03755:CDS
GGAGGTTGGAAACTCGAGTT+GGG 0.509671 4.3:+45125091 MS.gene03755:CDS
TGTTATGCAGTTTCTCGGCA+TGG 0.521848 4.3:+45124615 MS.gene03755:intron
TAACACTTAATAACAGAAGT+AGG 0.522805 4.3:-45124298 None:intergenic
GATCATTTGGATGCCTTCAA+GGG 0.544625 4.3:+45125705 MS.gene03755:CDS
GGTGGAGCCATTGATATCTC+CGG 0.546718 4.3:+45125112 MS.gene03755:CDS
AAATTCAAATGTAATGAGAC+TGG 0.547394 4.3:-45125147 None:intergenic
GAAATCCTCCGTACTAGTAT+GGG 0.549114 4.3:+45125310 MS.gene03755:CDS
GACGGGGCATTAATTTGTAG+TGG 0.550813 4.3:-45124322 None:intergenic
CAATTTGTATACAGTCCTGA+AGG 0.553354 4.3:+45124459 MS.gene03755:CDS
AGGAGGTTGGAAACTCGAGT+TGG 0.571978 4.3:+45125090 MS.gene03755:CDS
AACTCGTACCCATACTAGTA+CGG 0.577624 4.3:-45125318 None:intergenic
AGACTGGTTGTAAGACATGC+CGG 0.580136 4.3:-45125131 None:intergenic
AGAGAAAGATCTTCAAGTGA+CGG 0.585255 4.3:-45124340 None:intergenic
AAATATCTGAGAATTGCAGT+TGG 0.586019 4.3:+45124698 MS.gene03755:CDS
TCACTCAAGTAAGCTAGAGA+AGG 0.587930 4.3:+45125223 MS.gene03755:CDS
AGACATGCCGGAGATATCAA+TGG 0.591239 4.3:-45125119 None:intergenic
AATTCCCGAAGAAAGGAGGT+TGG 0.591474 4.3:+45125077 MS.gene03755:CDS
AATCAATTCCCGAAGAAAGG+AGG 0.619715 4.3:+45125073 MS.gene03755:CDS
GGTTGGAAACTCGAGTTGGG+TGG 0.621687 4.3:+45125094 MS.gene03755:CDS
AATTTGTATACAGTCCTGAA+GGG 0.640048 4.3:+45124460 MS.gene03755:CDS
TCGTACCCATACTAGTACGG+AGG 0.652397 4.3:-45125315 None:intergenic
AGAAAGATCTTCAAGTGACG+GGG 0.690406 4.3:-45124338 None:intergenic
TATGCAGTTTCTCGGCATGG+AGG 0.704407 4.3:+45124618 MS.gene03755:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATATTAATTTTTTTAT+CGG + chr4.3:45125598-45125617 MS.gene03755:intron 0.0%
!! TAAAATTAAGTATTCAATAT+TGG + chr4.3:45125658-45125677 MS.gene03755:intron 10.0%
!!! AAAATTCTAATGATAATTTT+TGG + chr4.3:45125028-45125047 MS.gene03755:intron 10.0%
!! ATGAAACTTGTAATTAAAAA+AGG - chr4.3:45124552-45124571 None:intergenic 15.0%
!! TCACAATTTATTATCTTTAT+CGG + chr4.3:45124672-45124691 MS.gene03755:CDS 15.0%
!!! TTGCACAATTTTTTTTTTAA+AGG + chr4.3:45124802-45124821 MS.gene03755:intron 15.0%
!! AAGTAAAATAGTTTGAAAGA+AGG - chr4.3:45124980-45124999 None:intergenic 20.0%
!! TAATTCAATTGAAGATCATT+TGG + chr4.3:45125692-45125711 MS.gene03755:CDS 20.0%
!! TATATATACTATTACATTCG+TGG + chr4.3:45125519-45125538 MS.gene03755:intron 20.0%
!! TTATCTTATATGCTATCATT+TGG + chr4.3:45125005-45125024 MS.gene03755:intron 20.0%
!!! ACTTTCTGTTTTAATGAAAA+AGG + chr4.3:45125377-45125396 MS.gene03755:intron 20.0%
!!! TTGGTATGTTAATATTTTGT+TGG + chr4.3:45125400-45125419 MS.gene03755:intron 20.0%
!!! TTTGTAAAGTCAAATTTTCT+AGG - chr4.3:45124385-45124404 None:intergenic 20.0%
! AAATTCAAATGTAATGAGAC+TGG - chr4.3:45125150-45125169 None:intergenic 25.0%
! AATATAATAAGCTCAAAACC+TGG + chr4.3:45124829-45124848 MS.gene03755:intron 25.0%
! ACTTTCTAATGTCAAATCAT+TGG + chr4.3:45124483-45124502 MS.gene03755:CDS 25.0%
! AGTTTGAAAGAAGGTTAAAT+TGG - chr4.3:45124971-45124990 None:intergenic 25.0%
! CTCATTACATTTGAATTTGT+TGG + chr4.3:45125152-45125171 MS.gene03755:CDS 25.0%
! TAACACTTAATAACAGAAGT+AGG - chr4.3:45124301-45124320 None:intergenic 25.0%
! TCAAAACATAAAAACTGAGT+TGG + chr4.3:45125178-45125197 MS.gene03755:CDS 25.0%
!! AAGTATTCAATATTGGTTAC+AGG + chr4.3:45125665-45125684 MS.gene03755:intron 25.0%
!!! TCTGTTTTAATGAAAAAGGT+TGG + chr4.3:45125381-45125400 MS.gene03755:intron 25.0%
!!! TTCTCTTGTTGATTTTTCAT+TGG + chr4.3:45124357-45124376 MS.gene03755:CDS 25.0%
AAATATCTGAGAATTGCAGT+TGG + chr4.3:45124698-45124717 MS.gene03755:CDS 30.0%
AATGTCTGTAAGAGTAAGAA+AGG - chr4.3:45125459-45125478 None:intergenic 30.0%
AATTTGTATACAGTCCTGAA+GGG + chr4.3:45124460-45124479 MS.gene03755:CDS 30.0%
AGTAGAAAAAAGTTTACGCA+AGG + chr4.3:45125280-45125299 MS.gene03755:CDS 30.0%
GAGATATAAAGTTGTGTTGA+TGG - chr4.3:45125490-45125509 None:intergenic 30.0%
GTAGAAAAAAGTTTACGCAA+GGG + chr4.3:45125281-45125300 MS.gene03755:CDS 30.0%
TACAAATTGTTAAAATCCGC+AGG - chr4.3:45124448-45124467 None:intergenic 30.0%
TAGAAAAAAGTTTACGCAAG+GGG + chr4.3:45125282-45125301 MS.gene03755:CDS 30.0%
TGAAAGAAGGTTAAATTGGA+AGG - chr4.3:45124967-45124986 None:intergenic 30.0%
! AACTATACGTATCTTTTGCT+AGG - chr4.3:45124872-45124891 None:intergenic 30.0%
! ATCTTTGTTATGCAGTTTCT+CGG + chr4.3:45124610-45124629 MS.gene03755:intron 30.0%
! ATTGCAAGATCCTTAAGTTT+TGG - chr4.3:45124754-45124773 None:intergenic 30.0%
! ATTTTGTTGGTATGATGTTG+TGG + chr4.3:45125413-45125432 MS.gene03755:intron 30.0%
! GAAAGTATATAGACTCTGTA+TGG - chr4.3:45125363-45125382 None:intergenic 30.0%
! TATTTTCATTTGTAAGCCTG+CGG + chr4.3:45124429-45124448 MS.gene03755:CDS 30.0%
! TTTTGTTGGTATGATGTTGT+GGG + chr4.3:45125414-45125433 MS.gene03755:intron 30.0%
!! TACTCTTCTTTTCTTTGAAG+TGG - chr4.3:45125264-45125283 None:intergenic 30.0%
!! TATAAAGTTGTGTTGATGGT+CGG - chr4.3:45125486-45125505 None:intergenic 30.0%
!!! ACTCTTCTTTTCTTTGAAGT+GGG - chr4.3:45125263-45125282 None:intergenic 30.0%
AATAGCAGCTTCATCAAGAT+TGG - chr4.3:45124413-45124432 None:intergenic 35.0%
ACACAATGCACCAAAACTTA+AGG + chr4.3:45124741-45124760 MS.gene03755:CDS 35.0%
ACTTAAGGATCTTGCAATTG+AGG + chr4.3:45124756-45124775 MS.gene03755:CDS 35.0%
AGAGAAAGATCTTCAAGTGA+CGG - chr4.3:45124343-45124362 None:intergenic 35.0%
CAATTTGTATACAGTCCTGA+AGG + chr4.3:45124459-45124478 MS.gene03755:CDS 35.0%
TGAAATCAATTCCCGAAGAA+AGG + chr4.3:45125070-45125089 MS.gene03755:CDS 35.0%
TTTGTTGGTATGATGTTGTG+GGG + chr4.3:45125415-45125434 MS.gene03755:intron 35.0%
AACTCGTACCCATACTAGTA+CGG - chr4.3:45125321-45125340 None:intergenic 40.0%
AATCAATTCCCGAAGAAAGG+AGG + chr4.3:45125073-45125092 MS.gene03755:CDS 40.0%
AGAAAGATCTTCAAGTGACG+GGG - chr4.3:45124341-45124360 None:intergenic 40.0%
AGAAATCCTCCGTACTAGTA+TGG + chr4.3:45125309-45125328 MS.gene03755:CDS 40.0%
AGATCATTTGGATGCCTTCA+AGG + chr4.3:45125704-45125723 MS.gene03755:CDS 40.0%
AGTTTCCAACCTCCTTTCTT+CGG - chr4.3:45125085-45125104 None:intergenic 40.0%
GAAATCCTCCGTACTAGTAT+GGG + chr4.3:45125310-45125329 MS.gene03755:CDS 40.0%
GAGAAAGATCTTCAAGTGAC+GGG - chr4.3:45124342-45124361 None:intergenic 40.0%
GAGATCTTCAATACCCTTGA+AGG - chr4.3:45125721-45125740 None:intergenic 40.0%
GATCATTTGGATGCCTTCAA+GGG + chr4.3:45125705-45125724 MS.gene03755:CDS 40.0%
TCACTCAAGTAAGCTAGAGA+AGG + chr4.3:45125223-45125242 MS.gene03755:CDS 40.0%
TGACATTAGAAAGTCCCTTC+AGG - chr4.3:45124477-45124496 None:intergenic 40.0%
AATTCCCGAAGAAAGGAGGT+TGG + chr4.3:45125077-45125096 MS.gene03755:CDS 45.0%
AGACATGCCGGAGATATCAA+TGG - chr4.3:45125122-45125141 None:intergenic 45.0%
GTTTCCAACCTCCTTTCTTC+GGG - chr4.3:45125084-45125103 None:intergenic 45.0%
TGTTATGCAGTTTCTCGGCA+TGG + chr4.3:45124615-45124634 MS.gene03755:intron 45.0%
! AGACTGGTTGTAAGACATGC+CGG - chr4.3:45125134-45125153 None:intergenic 45.0%
!! GACGGGGCATTAATTTGTAG+TGG - chr4.3:45124325-45124344 None:intergenic 45.0%
!!! ATATATTTAAAAATTTTAAG+AGG - chr4.3:45125586-45125605 None:intergenic 5.0%
AGGAGGTTGGAAACTCGAGT+TGG + chr4.3:45125090-45125109 MS.gene03755:CDS 50.0%
GGAGGTTGGAAACTCGAGTT+GGG + chr4.3:45125091-45125110 MS.gene03755:CDS 50.0%
GGTGGAGCCATTGATATCTC+CGG + chr4.3:45125112-45125131 MS.gene03755:CDS 50.0%
GTCTTTGCTCTTCTTGTGCC+AGG - chr4.3:45124850-45124869 None:intergenic 50.0%
GTTGGTATGATGTTGTGGGG+TGG + chr4.3:45125418-45125437 MS.gene03755:intron 50.0%
TATGCAGTTTCTCGGCATGG+AGG + chr4.3:45124618-45124637 MS.gene03755:intron 50.0%
TCGTACCCATACTAGTACGG+AGG - chr4.3:45125318-45125337 None:intergenic 50.0%
!! GGTTGGAAACTCGAGTTGGG+TGG + chr4.3:45125094-45125113 MS.gene03755:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 45124295 45125770 45124295 ID=MS.gene03755
chr4.3 mRNA 45124295 45125770 45124295 ID=MS.gene03755.t1;Parent=MS.gene03755
chr4.3 exon 45124295 45124528 45124295 ID=MS.gene03755.t1.exon1;Parent=MS.gene03755.t1
chr4.3 CDS 45124295 45124528 45124295 ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1
chr4.3 exon 45124625 45124777 45124625 ID=MS.gene03755.t1.exon2;Parent=MS.gene03755.t1
chr4.3 CDS 45124625 45124777 45124625 ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1
chr4.3 exon 45125062 45125331 45125062 ID=MS.gene03755.t1.exon3;Parent=MS.gene03755.t1
chr4.3 CDS 45125062 45125331 45125062 ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1
chr4.3 exon 45125687 45125770 45125687 ID=MS.gene03755.t1.exon4;Parent=MS.gene03755.t1
chr4.3 CDS 45125687 45125770 45125687 ID=cds.MS.gene03755.t1;Parent=MS.gene03755.t1
Gene Sequence

>MS.gene03755

ATGCCTACTTCTGTTATTAAGTGTTATCCACTACAAATTAATGCCCCGTCACTTGAAGATCTTTCTCTTGTTGATTTTTCATTGGCTCCTAGAAAATTTGACTTTACAAATTTGTCCAATCTTGATGAAGCTGCTATTTTCATTTGTAAGCCTGCGGATTTTAACAATTTGTATACAGTCCTGAAGGGACTTTCTAATGTCAAATCATTGGCTCTCGATTCTAACACTTTTCATGTAAGTTTCATAAATATTTTCCTTTTTTAATTACAAGTTTCATATTTAGTCACTCATATGCAATTCTATTCTTATTTTATCATCTTTGTTATGCAGTTTCTCGGCATGGAGGACAAACTTGACAACTTACATCTTCTCACTTTTCACAATTTATTATCTTTATCGGTTGAAATATCTGAGAATTGCAGTTGGAATATGCTTGTCAGCTTTCTACACAATGCACCAAAACTTAAGGATCTTGCAATTGAGGTGTGAATGATTTTTTAACTATATTTGCACAATTTTTTTTTTAAAGGATAAAATATAATAAGCTCAAAACCTGGCACAAGAAGAGCAAAGACCTAGCAAAAGATACGTATAGTTTGCAAATAAAACAGATCAACAAATTAATTAATTCAATGCAATCTATATATGATCTATATTTGTGTAGATTGTCCTTCCAATTTAACCTTCTTTCAAACTATTTTACTTACAATTTATCTTATATGCTATCATTTGGAAAATTCTAATGATAATTTTTGGTTTACATGCAGAGTAAAATTGAAATCAATTCCCGAAGAAAGGAGGTTGGAAACTCGAGTTGGGTGGAGCCATTGATATCTCCGGCATGTCTTACAACCAGTCTCATTACATTTGAATTTGTTGGAATTCAAAACATAAAAACTGAGTTGGACTTTACGCGATATATTGTAAGTCACTCAAGTAAGCTAGAGAAGGTAAAGATTTTCACACCCACTTCAAAGAAAAGAAGAGTAGAAAAAAGTTTACGCAAGGGGTCAAAGAAATCCTCCGTACTAGTATGGGTACGAGTTTTACACACTTTCTTTTCTTCCATACAGAGTCTATATACTTTCTGTTTTAATGAAAAAGGTTGGTATGTTAATATTTTGTTGGTATGATGTTGTGGGGTGGTGATAGAACTTTCATCCTTTCTTACTCTTACAGACATTAACTCCGACCATCAACACAACTTTATATCTCTTGAATGTATATATATACTATTACATTCGTGGTTGAGAATAAGACTGTTTTTCTTAAAATATGTAAATTATATCCTCTTAAAATTTTTAAATATATTAATTTTTTTATCGGTATACTTTAATGATTTTATCATTTATATTTATAATCTTAAAATTAAGTATTCAATATTGGTTACAGGATATTAATTCAATTGAAGATCATTTGGATGCCTTCAAGGGTATTGAAGATCTCTTTTTTGAGCTGTTCTTGCCCAATAAATAA

Protein sequence

>MS.gene03755.t1

MPTSVIKCYPLQINAPSLEDLSLVDFSLAPRKFDFTNLSNLDEAAIFICKPADFNNLYTVLKGLSNVKSLALDSNTFHFLGMEDKLDNLHLLTFHNLLSLSVEISENCSWNMLVSFLHNAPKLKDLAIESKIEINSRRKEVGNSSWVEPLISPACLTTSLITFEFVGIQNIKTELDFTRYIVSHSSKLEKVKIFTPTSKKRRVEKSLRKGSKKSSVLVWDINSIEDHLDAFKGIEDLFFELFLPNK