AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03758.t1 MTR_4g055990 79.783 460 81 2 31 485 47 499 0.0 712
MS.gene03758.t1 MTR_4g055580 78.526 475 94 2 9 483 116 582 0.0 711
MS.gene03758.t1 MTR_4g055990 81.693 437 75 1 31 462 47 483 0.0 702
MS.gene03758.t1 MTR_4g056040 78.049 451 92 2 9 459 25 468 0.0 689
MS.gene03758.t1 MTR_4g056070 85.422 391 54 1 31 418 47 437 0.0 672
MS.gene03758.t1 MTR_4g056020 74.286 455 73 3 31 485 47 457 0.0 653
MS.gene03758.t1 MTR_4g056030 70.064 471 97 3 9 479 25 451 0.0 625
MS.gene03758.t1 MTR_1g038710 60.746 456 134 6 9 458 35 451 2.37e-169 485
MS.gene03758.t1 MTR_7g110920 56.267 359 100 5 120 459 1 321 1.20e-119 353
MS.gene03758.t1 MTR_1g090883 55.652 345 112 5 31 373 57 362 5.75e-109 328
MS.gene03758.t1 MTR_4g056020 76.786 168 32 1 318 485 1 161 2.38e-84 257
MS.gene03758.t1 MTR_4g056010 91.000 100 9 0 89 188 1 100 8.43e-57 184
MS.gene03758.t1 MTR_4g093690 60.099 203 74 5 91 289 77 276 3.79e-56 188
MS.gene03758.t1 MTR_1g090900 44.615 195 64 4 8 195 20 177 4.09e-34 127
MS.gene03758.t1 MTR_1g090890 63.043 92 28 2 371 459 29 117 1.20e-30 115
MS.gene03758.t1 MTR_5g084070 28.912 377 226 15 97 458 78 427 4.38e-19 89.7
MS.gene03758.t1 MTR_8g014260 27.062 388 228 13 93 453 84 443 2.24e-17 84.7
MS.gene03758.t1 MTR_5g080710 29.630 351 211 12 97 437 101 425 2.61e-17 84.7
MS.gene03758.t1 MTR_8g445780 29.651 344 199 12 91 417 72 389 1.37e-15 79.0
MS.gene03758.t1 MTR_3g108560 30.675 326 142 17 117 432 105 356 1.39e-15 79.0
MS.gene03758.t1 MTR_8g062270 29.692 357 205 17 89 424 85 416 4.63e-15 77.8
MS.gene03758.t1 MTR_8g467800 29.189 370 209 16 79 421 69 412 1.58e-14 75.9
MS.gene03758.t1 MTR_8g468840 28.689 366 214 16 89 432 80 420 1.89e-14 75.9
MS.gene03758.t1 MTR_8g058970 27.132 387 209 15 90 432 85 442 2.96e-14 75.1
MS.gene03758.t1 MTR_8g468810 27.479 353 215 13 90 424 91 420 7.27e-14 73.9
MS.gene03758.t1 MTR_2g083840 30.278 360 197 17 89 424 85 414 9.23e-14 73.6
MS.gene03758.t1 MTR_5g008950 24.590 305 156 8 134 434 102 336 1.88e-13 72.4
MS.gene03758.t1 MTR_8g014160 27.062 388 195 15 83 454 103 418 3.26e-12 68.6
MS.gene03758.t1 MTR_7g056433 25.770 357 187 16 116 461 100 389 1.13e-11 67.0
MS.gene03758.t1 MTR_7g098570 25.723 346 189 11 91 432 79 360 2.57e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03758.t1 AT5G62970 32.931 331 183 18 114 432 113 416 6.07e-22 98.6
MS.gene03758.t1 AT4G00315 28.691 359 205 15 104 437 81 413 7.54e-20 92.4
MS.gene03758.t1 AT1G55660 27.298 359 210 15 104 437 132 464 6.11e-18 86.7
MS.gene03758.t1 AT4G14103 28.707 317 183 11 86 377 66 364 3.08e-17 84.0
MS.gene03758.t1 AT4G14103 28.707 317 183 11 86 377 66 364 3.08e-17 84.0
MS.gene03758.t1 AT1G51370 28.372 430 243 16 38 441 23 413 6.00e-17 83.6
MS.gene03758.t1 AT4G10400 27.174 368 227 12 96 455 69 403 8.04e-17 82.8
MS.gene03758.t1 AT4G10400 27.174 368 227 12 96 455 69 403 8.04e-17 82.8
MS.gene03758.t1 AT4G10400 27.174 368 227 12 96 455 69 403 8.04e-17 82.8
MS.gene03758.t1 AT4G14103 28.896 308 176 11 86 368 66 355 8.20e-17 83.2
MS.gene03758.t1 AT4G14096 29.043 303 181 12 86 371 66 351 1.20e-15 79.7
MS.gene03758.t1 AT4G14096 29.043 303 181 12 86 371 66 351 1.20e-15 79.7
MS.gene03758.t1 AT4G26340 26.316 342 222 12 102 437 74 391 2.47e-15 78.6
MS.gene03758.t1 AT5G56440 26.359 368 232 13 81 432 53 397 8.30e-15 76.6
MS.gene03758.t1 AT3G50710 27.296 392 230 17 66 437 41 397 1.17e-14 76.3
MS.gene03758.t1 AT5G56700 29.178 353 207 14 96 434 29 352 1.73e-14 75.5
MS.gene03758.t1 AT4G26350 26.087 345 206 13 113 437 85 400 4.07e-14 74.7
MS.gene03758.t1 AT3G59160 26.804 291 170 10 118 381 131 405 7.98e-14 73.9
MS.gene03758.t1 AT3G56780 25.490 408 212 16 117 457 90 472 1.06e-13 73.6
MS.gene03758.t1 AT1G51370 29.008 393 215 15 38 405 23 376 1.87e-13 72.4
MS.gene03758.t1 AT5G56410 28.940 349 203 16 114 446 106 425 2.32e-13 72.8
MS.gene03758.t1 AT3G59250 28.115 313 186 12 105 398 57 349 3.37e-13 71.6
MS.gene03758.t1 AT3G62430 29.373 303 173 14 88 368 59 342 3.43e-13 72.0
MS.gene03758.t1 AT3G59180 25.195 385 222 13 85 421 33 399 5.47e-13 71.2
MS.gene03758.t1 AT4G13965 29.677 310 176 15 145 443 125 403 3.25e-12 68.9
MS.gene03758.t1 AT1G32375 27.485 342 201 13 112 437 85 395 3.97e-12 68.6
MS.gene03758.t1 AT1G32375 27.485 342 201 13 112 437 85 395 3.97e-12 68.6
MS.gene03758.t1 AT3G59190 27.508 309 179 12 88 368 79 370 4.18e-12 68.9
MS.gene03758.t1 AT5G56370 26.884 398 227 17 89 470 57 406 5.21e-12 68.2
MS.gene03758.t1 AT5G56370 26.884 398 227 17 89 470 57 406 5.21e-12 68.2
MS.gene03758.t1 AT3G59190 27.508 309 179 12 88 368 79 370 7.05e-12 67.4
MS.gene03758.t1 AT5G38396 27.020 396 225 16 105 464 93 460 7.26e-12 67.8
MS.gene03758.t1 AT3G52680 26.706 337 198 11 115 434 105 409 8.53e-12 67.4
MS.gene03758.t1 AT1G58310 28.152 341 183 13 121 419 122 442 1.29e-11 67.0
MS.gene03758.t1 AT4G10420 25.146 342 222 10 120 454 1 315 1.68e-11 65.9
MS.gene03758.t1 AT4G00320 26.453 344 203 11 111 430 105 422 2.08e-11 66.6
MS.gene03758.t1 AT5G56390 25.987 304 187 9 141 434 119 394 2.64e-11 65.9
MS.gene03758.t1 AT5G56400 26.997 363 214 15 89 432 90 420 3.04e-11 65.9
MS.gene03758.t1 AT1G55660 29.310 232 129 11 104 316 132 347 9.58e-11 63.9

Find 87 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATTACATTTGAATTTAA+AGG 0.124626 4.3:+45149701 MS.gene03758:CDS
AGAAATCCTCCGTACTAGTA+TGG 0.259970 4.3:+45149858 MS.gene03758:CDS
ATTGCAAGATCCTTAAGTTT+TGG 0.269319 4.3:-45149279 None:intergenic
AGTTATTCAATCTTGGTTAC+AGG 0.281876 4.3:+45150260 MS.gene03758:intron
TGACATCAAGATTTAAAGAT+TGG 0.308638 4.3:-45148270 None:intergenic
AGTTTCCAACCTCCTTTCTT+CGG 0.314970 4.3:-45149631 None:intergenic
TAAAATTAGTTATTCAATCT+TGG 0.319018 4.3:+45150253 MS.gene03758:intron
GGTCTCAATAATTTCCTATC+TGG 0.337995 4.3:+45148712 MS.gene03758:CDS
GTTTCCAACCTCCTTTCTTC+GGG 0.346723 4.3:-45149630 None:intergenic
AATTCAATTTGAAGATCATT+TGG 0.348696 4.3:+45150288 MS.gene03758:CDS
CCTATCTGGTTGTCGACATC+TGG 0.349992 4.3:+45148726 MS.gene03758:CDS
AGATCATTTGGATTCGTTCA+TGG 0.377422 4.3:+45150300 MS.gene03758:CDS
ACTCGTGGTCTTAAAATTGC+GGG 0.408358 4.3:+45148576 MS.gene03758:CDS
ACTTTCTAATGTCAAATCAT+TGG 0.410912 4.3:+45149020 MS.gene03758:CDS
GCTGAATGCTCCAAAACTTA+AGG 0.411850 4.3:+45149269 MS.gene03758:CDS
ACTTAAGGATCTTGCAATTG+AGG 0.413085 4.3:+45149284 MS.gene03758:CDS
ACAGATCCAAGTCAAGTTCC+TGG 0.422138 4.3:-45148504 None:intergenic
GAAACTTACATGATAAGTAT+TGG 0.428489 4.3:-45149052 None:intergenic
GGTAATTGAAAGTGAAGGTT+GGG 0.443422 4.3:-45148211 None:intergenic
TTTGATGATGGTATGGAGTA+TGG 0.443571 4.3:+45148691 MS.gene03758:CDS
AAGAGTAGCTCGATCAAGAT+TGG 0.446848 4.3:-45148947 None:intergenic
TAGAAAGAACTTCGAGTGAC+GGG 0.452168 4.3:-45148876 None:intergenic
TAACAATTTGTATACACTCT+TGG 0.456119 4.3:+45148993 MS.gene03758:CDS
CAAGGTCCAGGAACTTGACT+TGG 0.457085 4.3:+45148498 MS.gene03758:CDS
TAGAATACATCATAGTTTCA+TGG 0.460494 4.3:-45148666 None:intergenic
CTAAGGCTTGATAGTGAGAA+AGG 0.463856 4.3:+45148319 MS.gene03758:CDS
TCTTTCCATTTGTAAGCGTC+CGG 0.469789 4.3:+45148966 MS.gene03758:CDS
CGGAGGATTTCTTTGACCCC+TGG 0.474526 4.3:-45149847 None:intergenic
CCAGATGTCGACAACCAGAT+AGG 0.477978 4.3:-45148726 None:intergenic
GTATACAAATTGTTAAAATC+CGG 0.478212 4.3:-45148985 None:intergenic
TTCTATCTTTGATGATGGTA+TGG 0.482209 4.3:+45148684 MS.gene03758:CDS
TGAAATCAATTCCCGAAGAA+AGG 0.482837 4.3:+45149619 MS.gene03758:CDS
AAAATCCGGACGCTTACAAA+TGG 0.495967 4.3:-45148971 None:intergenic
GTTCATACACATGCTACAAA+TGG 0.501841 4.3:+45147214 MS.gene03758:CDS
AATTTGTATACACTCTTGGA+AGG 0.503360 4.3:+45148997 MS.gene03758:CDS
TACACATGCTACAAATGGTC+AGG 0.508193 4.3:+45147219 MS.gene03758:CDS
GGAGGTTGGAAACTCGAGTT+GGG 0.509671 4.3:+45149640 MS.gene03758:CDS
TTCAAAGGCATTACATCTCA+AGG 0.521815 4.3:+45148480 MS.gene03758:CDS
ATGTATTCTATCTTTGATGA+TGG 0.523217 4.3:+45148679 MS.gene03758:CDS
TGTACACATGTGGGTTTCAA+AGG 0.528762 4.3:+45148357 MS.gene03758:CDS
TGTACACATGTGGGTTTCAA+AGG 0.528762 4.3:+45148465 MS.gene03758:CDS
GAAATCCTCCGTACTAGTAT+GGG 0.530507 4.3:+45149859 MS.gene03758:CDS
GGCATTACATCTCAAGGTCC+AGG 0.533334 4.3:+45148486 MS.gene03758:CDS
CAACACTTAATAACAGAAGT+AGG 0.536563 4.3:-45148835 None:intergenic
CCGAAAATATAAGAAAACTA+AGG 0.538047 4.3:+45148410 MS.gene03758:CDS
AAGTAGGCATACATAAAGAA+AGG 0.538450 4.3:-45148819 None:intergenic
CACTCGTGGTCTTAAAATTG+CGG 0.539007 4.3:+45148575 MS.gene03758:CDS
ACATGTGTACATCATGTTGC+TGG 0.545128 4.3:-45148345 None:intergenic
CTAAGGCTTGATAGCGACAA+AGG 0.546051 4.3:+45148427 MS.gene03758:CDS
GCTACAAATGGTCAGGCTCA+TGG 0.549836 4.3:+45147226 MS.gene03758:CDS
AACACAGGGTCTGAAATCTC+TGG 0.550462 4.3:-45148232 None:intergenic
TGGTAATTGAAAGTGAAGGT+TGG 0.551378 4.3:-45148212 None:intergenic
AATGTCAAATCATTGGCTCT+CGG 0.552626 4.3:+45149027 MS.gene03758:CDS
TAAGTGAAAAGAGATGTTGA+TGG 0.554261 4.3:-45148782 None:intergenic
GACGGGGCGTTAATTTGTAG+TGG 0.565806 4.3:-45148859 None:intergenic
AGGAGGTTGGAAACTCGAGT+TGG 0.571978 4.3:+45149639 MS.gene03758:CDS
AGACTGCTTGTAAGACATGC+CGG 0.577653 4.3:-45149680 None:intergenic
AAGTGAAAAGAGATGTTGAT+GGG 0.577961 4.3:-45148781 None:intergenic
ATTACATACCCATACTAGTA+CGG 0.586209 4.3:-45149867 None:intergenic
AAATATCTGAGAGTTGCAGT+TGG 0.588124 4.3:+45149226 MS.gene03758:CDS
TGAAAAGAAGAATTGAGTGT+TGG 0.588267 4.3:-45148610 None:intergenic
AATTCCCGAAGAAAGGAGGT+TGG 0.591474 4.3:+45149626 MS.gene03758:CDS
GAAATGGGAAATGAATATGA+TGG 0.591870 4.3:+45147181 None:intergenic
GGTGGAGCCATCAGTAACTC+CGG 0.593298 4.3:+45149661 MS.gene03758:CDS
ATCTGGTTGTCGACATCTGG+AGG 0.596091 4.3:+45148729 MS.gene03758:CDS
ATAGAAAGAACTTCGAGTGA+CGG 0.607597 4.3:-45148877 None:intergenic
ATTCTTCTTTAGACAACACA+GGG 0.608058 4.3:-45148246 None:intergenic
AATTCTTCTTTAGACAACAC+AGG 0.613061 4.3:-45148247 None:intergenic
AGACATGCCGGAGTTACTGA+TGG 0.614504 4.3:-45149668 None:intergenic
AATCAATTCCCGAAGAAAGG+AGG 0.619715 4.3:+45149622 MS.gene03758:CDS
TCACTCAAGTAAGTTAGCGA+AGG 0.620983 4.3:+45149772 MS.gene03758:CDS
GGTTGGAAACTCGAGTTGGG+TGG 0.621687 4.3:+45149643 MS.gene03758:CDS
TTCCACGTGTGAGTCACTCG+TGG 0.631019 4.3:+45148561 MS.gene03758:CDS
GCCACATGATGTACACATGT+GGG 0.650684 4.3:+45148456 MS.gene03758:CDS
ACATGTGTACATCATGTGGC+TGG 0.656362 4.3:-45148453 None:intergenic
ACATACCCATACTAGTACGG+AGG 0.663039 4.3:-45149864 None:intergenic
AGAAAGAACTTCGAGTGACG+GGG 0.663199 4.3:-45148875 None:intergenic
AGATGAAGAATCTTTAGTGA+CGG 0.666233 4.3:-45148643 None:intergenic
GCAACATGATGTACACATGT+GGG 0.668046 4.3:+45148348 MS.gene03758:CDS
ACCCACATGTGTACATCATG+TGG 0.672342 4.3:-45148457 None:intergenic
CTCGTGGTCTTAAAATTGCG+GGG 0.673910 4.3:+45148577 MS.gene03758:CDS
CCTAGATTAAAAGTCTCGAG+TGG 0.677340 4.3:-45150335 None:intergenic
AGCAACATGATGTACACATG+TGG 0.678239 4.3:+45148347 MS.gene03758:CDS
GACCACGAGTGACTCACACG+TGG 0.682798 4.3:-45148563 None:intergenic
AGCCACATGATGTACACATG+TGG 0.685172 4.3:+45148455 MS.gene03758:CDS
TCTCTGGTAATTGAAAGTGA+AGG 0.696256 4.3:-45148216 None:intergenic
TCACACGTGGAAAGCCTAAG+CGG 0.701582 4.3:-45148550 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATATTAATATTTTTAT+CGG + chr4.3:45150193-45150212 MS.gene03758:intron 0.0%
!! AATTAAAATAGTTTAAAAGA+AGG - chr4.3:45149500-45149519 None:intergenic 10.0%
!! ATAACAAATATTTATGTATT+TGG - chr4.3:45147284-45147303 None:intergenic 10.0%
!!! AAAATTCTAATGATAATTTT+TGG + chr4.3:45149581-45149600 MS.gene03758:intron 10.0%
!!! GAATTAATTAATTTGTTTAT+CGG - chr4.3:45149437-45149456 None:intergenic 10.0%
!! ACGAAAATATAAAAAAACTA+AGG + chr4.3:45148302-45148321 MS.gene03758:CDS 15.0%
!! ATAACATCATTAATATATCA+AGG - chr4.3:45147507-45147526 None:intergenic 15.0%
!! ATGTAAATGAAGATAAAAAT+TGG - chr4.3:45149523-45149542 None:intergenic 15.0%
!! TAAAATTAGTTATTCAATCT+TGG + chr4.3:45150253-45150272 MS.gene03758:intron 15.0%
!! TAGAAAAAGATAGAAAATAA+AGG - chr4.3:45147904-45147923 None:intergenic 15.0%
!! TATATTAATGATGTTATCTT+TGG + chr4.3:45147510-45147529 MS.gene03758:intron 15.0%
!!! AATTTTTTAATAACGTATTG+AGG + chr4.3:45147687-45147706 MS.gene03758:intron 15.0%
!!! TCTTTTTTAAAGAAAAAAGT+TGG + chr4.3:45149930-45149949 MS.gene03758:intron 15.0%
!! AAAGTAACAAATCTTAGAAA+TGG - chr4.3:45147946-45147965 None:intergenic 20.0%
!! AAGTAACAAATCTTAGAAAT+GGG - chr4.3:45147945-45147964 None:intergenic 20.0%
!! AATATAAGTGAAAAATGACT+GGG - chr4.3:45147877-45147896 None:intergenic 20.0%
!! AATTCAATTTGAAGATCATT+TGG + chr4.3:45150288-45150307 MS.gene03758:CDS 20.0%
!! ACTATTTACTATATATCAGT+TGG + chr4.3:45149558-45149577 MS.gene03758:intron 20.0%
!! AGTTTAAAAGAAGGTTAAAT+TGG - chr4.3:45149491-45149510 None:intergenic 20.0%
!! ATGGAACTTGTAATTAAAAA+AGG - chr4.3:45149089-45149108 None:intergenic 20.0%
!! CTCATTACATTTGAATTTAA+AGG + chr4.3:45149701-45149720 MS.gene03758:CDS 20.0%
!! GTATACAAATTGTTAAAATC+CGG - chr4.3:45148988-45149007 None:intergenic 20.0%
!! TATATCAAGGTATAATAACA+AGG - chr4.3:45147494-45147513 None:intergenic 20.0%
!!! CCTTAGTTTTCTTATATTTT+CGG - chr4.3:45148413-45148432 None:intergenic 20.0%
!!! TTTCTAAGATTTGTTACTTT+TGG + chr4.3:45147946-45147965 MS.gene03758:intron 20.0%
! ACTTTCTAATGTCAAATCAT+TGG + chr4.3:45149020-45149039 MS.gene03758:CDS 25.0%
! ATGTATTCTATCTTTGATGA+TGG + chr4.3:45148679-45148698 MS.gene03758:CDS 25.0%
! CCGAAAATATAAGAAAACTA+AGG + chr4.3:45148410-45148429 MS.gene03758:CDS 25.0%
! GAAACTTACATGATAAGTAT+TGG - chr4.3:45149055-45149074 None:intergenic 25.0%
! GAATATAAGTGAAAAATGAC+TGG - chr4.3:45147878-45147897 None:intergenic 25.0%
! TAAAAGAAGGTTAAATTGGT+AGG - chr4.3:45149487-45149506 None:intergenic 25.0%
! TAACAATTTGTATACACTCT+TGG + chr4.3:45148993-45149012 MS.gene03758:CDS 25.0%
! TAGAATACATCATAGTTTCA+TGG - chr4.3:45148669-45148688 None:intergenic 25.0%
! TCAAAACATAAAAACTGAGT+TGG + chr4.3:45149727-45149746 MS.gene03758:CDS 25.0%
! TGACATCAAGATTTAAAGAT+TGG - chr4.3:45148273-45148292 None:intergenic 25.0%
! TTAAGATAGAAGTTTGTCTT+AGG - chr4.3:45147383-45147402 None:intergenic 25.0%
! TTAATGATGTTATCTTTGGA+TGG + chr4.3:45147514-45147533 MS.gene03758:intron 25.0%
!! TATAACACTCTTTTGTTGAT+AGG - chr4.3:45148143-45148162 None:intergenic 25.0%
AAGTAGGCATACATAAAGAA+AGG - chr4.3:45148822-45148841 None:intergenic 30.0%
AAGTGAAAAGAGATGTTGAT+GGG - chr4.3:45148784-45148803 None:intergenic 30.0%
AATAGACTTGCATCTACATA+TGG - chr4.3:45149108-45149127 None:intergenic 30.0%
AATTCTTCTTTAGACAACAC+AGG - chr4.3:45148250-45148269 None:intergenic 30.0%
AATTTGTATACACTCTTGGA+AGG + chr4.3:45148997-45149016 MS.gene03758:CDS 30.0%
AGATGAAGAATCTTTAGTGA+CGG - chr4.3:45148646-45148665 None:intergenic 30.0%
AGTTATTCAATCTTGGTTAC+AGG + chr4.3:45150260-45150279 MS.gene03758:intron 30.0%
ATTACATACCCATACTAGTA+CGG - chr4.3:45149870-45149889 None:intergenic 30.0%
ATTCTTCTTTAGACAACACA+GGG - chr4.3:45148249-45148268 None:intergenic 30.0%
CAACACTTAATAACAGAAGT+AGG - chr4.3:45148838-45148857 None:intergenic 30.0%
CTACCTGAACAAAAAAACTA+TGG + chr4.3:45147427-45147446 MS.gene03758:intron 30.0%
GTTGGCATGTTAATATTTGT+TGG + chr4.3:45149948-45149967 MS.gene03758:intron 30.0%
TAAGTGAAAAGAGATGTTGA+TGG - chr4.3:45148785-45148804 None:intergenic 30.0%
TATCTTAAAGCTTGCAACAA+TGG + chr4.3:45147395-45147414 MS.gene03758:intron 30.0%
TCAAAATTCCCAATACGATA+CGG - chr4.3:45147713-45147732 None:intergenic 30.0%
TGAAAAGAAGAATTGAGTGT+TGG - chr4.3:45148613-45148632 None:intergenic 30.0%
TGACTTATTATTGTCCAGTT+CGG + chr4.3:45147324-45147343 MS.gene03758:intron 30.0%
! AAAAAATCTTATTGCCGCTT+AGG + chr4.3:45148536-45148555 MS.gene03758:CDS 30.0%
! AATGTCTAGAACAATAAGAG+AGG - chr4.3:45150007-45150026 None:intergenic 30.0%
! ACTCTTCTTTTCTTTGAACT+AGG - chr4.3:45149812-45149831 None:intergenic 30.0%
! ATTGCAAGATCCTTAAGTTT+TGG - chr4.3:45149282-45149301 None:intergenic 30.0%
! ATTTGTTGGTATGATGTTGT+GGG + chr4.3:45149962-45149981 MS.gene03758:intron 30.0%
! CTTGCAAACTCATATTTTCT+AGG - chr4.3:45148922-45148941 None:intergenic 30.0%
! GAAAGTATATAAACTCTGTG+TGG - chr4.3:45149912-45149931 None:intergenic 30.0%
! TATTTGTTGGTATGATGTTG+TGG + chr4.3:45149961-45149980 MS.gene03758:intron 30.0%
!! TTCTATCTTTGATGATGGTA+TGG + chr4.3:45148684-45148703 MS.gene03758:CDS 30.0%
!!! CAACCATAGTTTTTTTGTTC+AGG - chr4.3:45147433-45147452 None:intergenic 30.0%
!!! CATAGTTTTTTTGTTCAGGT+AGG - chr4.3:45147429-45147448 None:intergenic 30.0%
AAATATCTGAGAGTTGCAGT+TGG + chr4.3:45149226-45149245 MS.gene03758:CDS 35.0%
ACTTAAGGATCTTGCAATTG+AGG + chr4.3:45149284-45149303 MS.gene03758:CDS 35.0%
AGATCATTTGGATTCGTTCA+TGG + chr4.3:45150300-45150319 MS.gene03758:CDS 35.0%
AGCTGTTAAATGTAGTAGTG+TGG - chr4.3:45147800-45147819 None:intergenic 35.0%
AGTAGAAAAAAGTTTACGCC+AGG + chr4.3:45149829-45149848 MS.gene03758:CDS 35.0%
ATAAGCTCAAAATCTAGCAC+AGG + chr4.3:45149366-45149385 MS.gene03758:intron 35.0%
ATAGAAAGAACTTCGAGTGA+CGG - chr4.3:45148880-45148899 None:intergenic 35.0%
ATGTGTTCTATCCACAAGAT+GGG + chr4.3:45148167-45148186 MS.gene03758:intron 35.0%
GCTGTTAAATGTAGTAGTGT+GGG - chr4.3:45147799-45147818 None:intergenic 35.0%
GGTAATTGAAAGTGAAGGTT+GGG - chr4.3:45148214-45148233 None:intergenic 35.0%
GGTCTCAATAATTTCCTATC+TGG + chr4.3:45148712-45148731 MS.gene03758:CDS 35.0%
GTAGAAAAAAGTTTACGCCA+GGG + chr4.3:45149830-45149849 MS.gene03758:CDS 35.0%
GTTCATACACATGCTACAAA+TGG + chr4.3:45147214-45147233 MS.gene03758:CDS 35.0%
TAGAAAAAAGTTTACGCCAG+GGG + chr4.3:45149831-45149850 MS.gene03758:CDS 35.0%
TATTAGTATCGTATCGTGTC+AGG + chr4.3:45147744-45147763 MS.gene03758:intron 35.0%
TCTCTGGTAATTGAAAGTGA+AGG - chr4.3:45148219-45148238 None:intergenic 35.0%
TGAAATCAATTCCCGAAGAA+AGG + chr4.3:45149619-45149638 MS.gene03758:CDS 35.0%
TGGTAATTGAAAGTGAAGGT+TGG - chr4.3:45148215-45148234 None:intergenic 35.0%
TTTGTTGGTATGATGTTGTG+GGG + chr4.3:45149963-45149982 MS.gene03758:intron 35.0%
!! AATGTCAAATCATTGGCTCT+CGG + chr4.3:45149027-45149046 MS.gene03758:CDS 35.0%
!! TTATATTTTCGGTGCGTCTT+AGG - chr4.3:45148402-45148421 None:intergenic 35.0%
!! TTCAAAGGCATTACATCTCA+AGG + chr4.3:45148480-45148499 MS.gene03758:CDS 35.0%
!! TTTGATGATGGTATGGAGTA+TGG + chr4.3:45148691-45148710 MS.gene03758:CDS 35.0%
!!! GCGGCAATAAGATTTTTTCA+CGG - chr4.3:45148534-45148553 None:intergenic 35.0%
AAAATCCGGACGCTTACAAA+TGG - chr4.3:45148974-45148993 None:intergenic 40.0%
AAGAGTAGCTCGATCAAGAT+TGG - chr4.3:45148950-45148969 None:intergenic 40.0%
AATCAATTCCCGAAGAAAGG+AGG + chr4.3:45149622-45149641 MS.gene03758:CDS 40.0%
ACATGTGTACATCATGTTGC+TGG - chr4.3:45148348-45148367 None:intergenic 40.0%
AGAAATCCTCCGTACTAGTA+TGG + chr4.3:45149858-45149877 MS.gene03758:CDS 40.0%
AGCAACATGATGTACACATG+TGG + chr4.3:45148347-45148366 MS.gene03758:CDS 40.0%
AGTTTCCAACCTCCTTTCTT+CGG - chr4.3:45149634-45149653 None:intergenic 40.0%
CATAACTTTACTCCGCTGTT+CGG + chr4.3:45148035-45148054 MS.gene03758:intron 40.0%
CATGTGTTCTATCCACAAGA+TGG + chr4.3:45148166-45148185 MS.gene03758:intron 40.0%
CTTAAAGCTTGCAACAATGG+AGG + chr4.3:45147398-45147417 MS.gene03758:intron 40.0%
GAAATCCTCCGTACTAGTAT+GGG + chr4.3:45149859-45149878 MS.gene03758:CDS 40.0%
GCAACATGATGTACACATGT+GGG + chr4.3:45148348-45148367 MS.gene03758:CDS 40.0%
GCTGAATGCTCCAAAACTTA+AGG + chr4.3:45149269-45149288 MS.gene03758:CDS 40.0%
TAAATGTAGTAGTGTGGGTG+AGG - chr4.3:45147794-45147813 None:intergenic 40.0%
TACACATGCTACAAATGGTC+AGG + chr4.3:45147219-45147238 MS.gene03758:CDS 40.0%
TAGAAAGAACTTCGAGTGAC+GGG - chr4.3:45148879-45148898 None:intergenic 40.0%
TATCGTATCGTGTCAGGTAT+CGG + chr4.3:45147750-45147769 MS.gene03758:intron 40.0%
TATTGAGGCCGTATCGTATT+GGG + chr4.3:45147702-45147721 MS.gene03758:intron 40.0%
TCACTCAAGTAAGTTAGCGA+AGG + chr4.3:45149772-45149791 MS.gene03758:CDS 40.0%
TCTTTCCATTTGTAAGCGTC+CGG + chr4.3:45148966-45148985 MS.gene03758:CDS 40.0%
TGATAGAGAAAGAACCGAAC+TGG - chr4.3:45147341-45147360 None:intergenic 40.0%
TGTACACATGTGGGTTTCAA+AGG + chr4.3:45148357-45148376 MS.gene03758:CDS 40.0%
! ACTCGTGGTCTTAAAATTGC+GGG + chr4.3:45148576-45148595 MS.gene03758:CDS 40.0%
! CACTCGTGGTCTTAAAATTG+CGG + chr4.3:45148575-45148594 MS.gene03758:CDS 40.0%
! TCTTAGGTTGCTCAAGCTAA+TGG - chr4.3:45147367-45147386 None:intergenic 40.0%
!! CTAAGGCTTGATAGTGAGAA+AGG + chr4.3:45148319-45148338 MS.gene03758:CDS 40.0%
AATTCCCGAAGAAAGGAGGT+TGG + chr4.3:45149626-45149645 MS.gene03758:CDS 45.0%
ACAGATCCAAGTCAAGTTCC+TGG - chr4.3:45148507-45148526 None:intergenic 45.0%
ACATACCCATACTAGTACGG+AGG - chr4.3:45149867-45149886 None:intergenic 45.0%
ACATGTGTACATCATGTGGC+TGG - chr4.3:45148456-45148475 None:intergenic 45.0%
ACCCACATGTGTACATCATG+TGG - chr4.3:45148352-45148371 None:intergenic 45.0%
AGAAAGAACTTCGAGTGACG+GGG - chr4.3:45148878-45148897 None:intergenic 45.0%
AGACTGCTTGTAAGACATGC+CGG - chr4.3:45149683-45149702 None:intergenic 45.0%
AGCCACATGATGTACACATG+TGG + chr4.3:45148455-45148474 MS.gene03758:CDS 45.0%
ATCGTATCGTGTCAGGTATC+GGG + chr4.3:45147751-45147770 MS.gene03758:intron 45.0%
ATCTAGCACAGGAAGAGCAA+AGG + chr4.3:45149377-45149396 MS.gene03758:intron 45.0%
GCCACATGATGTACACATGT+GGG + chr4.3:45148456-45148475 MS.gene03758:CDS 45.0%
GTATTGAGGCCGTATCGTAT+TGG + chr4.3:45147701-45147720 MS.gene03758:intron 45.0%
GTTTCCAACCTCCTTTCTTC+GGG - chr4.3:45149633-45149652 None:intergenic 45.0%
! AACACAGGGTCTGAAATCTC+TGG - chr4.3:45148235-45148254 None:intergenic 45.0%
! CTCGTGGTCTTAAAATTGCG+GGG + chr4.3:45148577-45148596 MS.gene03758:CDS 45.0%
!! CTAAGGCTTGATAGCGACAA+AGG + chr4.3:45148427-45148446 MS.gene03758:CDS 45.0%
!! TATAATTAAAAAAATTTAAG+AGG - chr4.3:45150180-45150199 None:intergenic 5.0%
AGACATGCCGGAGTTACTGA+TGG - chr4.3:45149671-45149690 None:intergenic 50.0%
AGGAGGTTGGAAACTCGAGT+TGG + chr4.3:45149639-45149658 MS.gene03758:CDS 50.0%
AGTGTGTGTGTGTGTGTGTG+TGG - chr4.3:45150038-45150057 None:intergenic 50.0%
ATCTGGTTGTCGACATCTGG+AGG + chr4.3:45148729-45148748 MS.gene03758:CDS 50.0%
CAAGGTCCAGGAACTTGACT+TGG + chr4.3:45148498-45148517 MS.gene03758:CDS 50.0%
CCAGATGTCGACAACCAGAT+AGG - chr4.3:45148729-45148748 None:intergenic 50.0%
CCTATCTGGTTGTCGACATC+TGG + chr4.3:45148726-45148745 MS.gene03758:CDS 50.0%
GCTACAAATGGTCAGGCTCA+TGG + chr4.3:45147226-45147245 MS.gene03758:CDS 50.0%
GGAGGTTGGAAACTCGAGTT+GGG + chr4.3:45149640-45149659 MS.gene03758:CDS 50.0%
GGCATTACATCTCAAGGTCC+AGG + chr4.3:45148486-45148505 MS.gene03758:CDS 50.0%
GTGTGTGTCTGTGTGTGTGT+GGG - chr4.3:45150064-45150083 None:intergenic 50.0%
GTTGGTATGATGTTGTGGGG+TGG + chr4.3:45149966-45149985 MS.gene03758:intron 50.0%
TCACACGTGGAAAGCCTAAG+CGG - chr4.3:45148553-45148572 None:intergenic 50.0%
TGTGTGTCTGTGTGTGTGTG+GGG - chr4.3:45150063-45150082 None:intergenic 50.0%
TGTGTGTGTCTGTGTGTGTG+TGG - chr4.3:45150065-45150084 None:intergenic 50.0%
TTTACTCCGCTGTTCGGCTT+AGG + chr4.3:45148041-45148060 MS.gene03758:intron 50.0%
! GCAGATTGACTCCCATCTTG+TGG - chr4.3:45148181-45148200 None:intergenic 50.0%
! TCGTATCGTGTCAGGTATCG+GGG + chr4.3:45147752-45147771 MS.gene03758:intron 50.0%
!! GACGGGGCGTTAATTTGTAG+TGG - chr4.3:45148862-45148881 None:intergenic 50.0%
CCATGACCTAAGCCGAACAG+CGG - chr4.3:45148050-45148069 None:intergenic 55.0%
CGGAGGATTTCTTTGACCCC+TGG - chr4.3:45149850-45149869 None:intergenic 55.0%
GGTGGAGCCATCAGTAACTC+CGG + chr4.3:45149661-45149680 MS.gene03758:CDS 55.0%
TTCCACGTGTGAGTCACTCG+TGG + chr4.3:45148561-45148580 MS.gene03758:CDS 55.0%
! GGTATCGGGGCTTCATAGCT+TGG + chr4.3:45147765-45147784 MS.gene03758:intron 55.0%
!! GGTTGGAAACTCGAGTTGGG+TGG + chr4.3:45149643-45149662 MS.gene03758:CDS 55.0%
CCGCTGTTCGGCTTAGGTCA+TGG + chr4.3:45148047-45148066 MS.gene03758:intron 60.0%
GACCACGAGTGACTCACACG+TGG - chr4.3:45148566-45148585 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 45147191 45150351 45147191 ID=MS.gene03758
chr4.3 mRNA 45147191 45150351 45147191 ID=MS.gene03758.t1;Parent=MS.gene03758
chr4.3 exon 45147191 45147260 45147191 ID=MS.gene03758.t1.exon1;Parent=MS.gene03758.t1
chr4.3 CDS 45147191 45147260 45147191 ID=cds.MS.gene03758.t1;Parent=MS.gene03758.t1
chr4.3 exon 45148185 45149065 45148185 ID=MS.gene03758.t1.exon2;Parent=MS.gene03758.t1
chr4.3 CDS 45148185 45149065 45148185 ID=cds.MS.gene03758.t1;Parent=MS.gene03758.t1
chr4.3 exon 45149153 45149305 45149153 ID=MS.gene03758.t1.exon3;Parent=MS.gene03758.t1
chr4.3 CDS 45149153 45149305 45149153 ID=cds.MS.gene03758.t1;Parent=MS.gene03758.t1
chr4.3 exon 45149611 45149880 45149611 ID=MS.gene03758.t1.exon4;Parent=MS.gene03758.t1
chr4.3 CDS 45149611 45149880 45149611 ID=cds.MS.gene03758.t1;Parent=MS.gene03758.t1
chr4.3 exon 45150262 45150351 45150262 ID=MS.gene03758.t1.exon5;Parent=MS.gene03758.t1
chr4.3 CDS 45150262 45150351 45150262 ID=cds.MS.gene03758.t1;Parent=MS.gene03758.t1
Gene Sequence

>MS.gene03758

ATGAATATGATGGTCACATACATGTTCATACACATGCTACAAATGGTCAGGCTCATGGTTCAACTAACAAGTAAGATCAATGAGTTGTTGCCAAATACATAAATATTTGTTATTTGTTTATTTTGTTTTTGTTTGACTTATTATTGTCCAGTTCGGTTCTTTCTCTATCATTACCATTAGCTTGAGCAACCTAAGACAAACTTCTATCTTAAAGCTTGCAACAATGGAGGAAGAACCTACCTGAACAAAAAAACTATGGTTGTCTTTGTTGTTAAGTAATTTTTATTTCAAAATGTATAGCCTTGTTATTATACCTTGATATATTAATGATGTTATCTTTGGATGGTACTGATGATGTTTAACTATATTCTAATTTGTGCTGAAAAAGTATGTAATTGTCACTTGTTTTGATCATTTTCATTAATGTGAATGTTTGTTTATTATCATTTTAAGTTATATTTATACATTGTGCATTTATACTATTAATTACAAATTTAATTTTTTAATAACGTATTGAGGCCGTATCGTATTGGGAATTTTGAAAAACTTCTCGTATTAGTATCGTATCGTGTCAGGTATCGGGGCTTCATAGCTTGGCAACCTCACCCACACTACTACATTTAACAGCTTCAGTAACATTTTTAATGTAACATTTGTAAAATCATGTCTAACATTTTTCATTGCCCAGTCATTTTTCACTTATATTCATTCCTTTATTTTCTATCTTTTTCTATTCATTGATCATTTTGTACCCATTTCTAAGATTTGTTACTTTTGGAGTTAATTTTTTTTGAAATTGTGTTAATCTCTCACTTTATTGCTAATGAAATCACTTCAATCATGTCATAACTTTACTCCGCTGTTCGGCTTAGGTCATGGCGTGCTCTGATGAAGAACATGATCGATTAAGCAATCTCTCAGACGATTTGATTTGTCATATTTTGTCTTTCCTATCAACAAAAGAGTGTTATAGAACATGTGTTCTATCCACAAGATGGGAGTCAATCTGCACACAAGATTCCCAACCTTCACTTTCAATTACCAGAGATTTCAGACCCTGTGTTGTCTAAAGAAGAATTCCAATCTTTAAATCTTGATGTCATAAGACGCAACGAAAATATAAAAAAACTAAGGCTTGATAGTGAGAAAGGCTGCCAGCAACATGATGTACACATGTGGGTTTCAAAGGCATTTGTAAATTTTGCTCTCCTAAGACGCACCGAAAATATAAGAAAACTAAGGCTTGATAGCGACAAAGGCTGCCAGCCACATGATGTACACATGTGGGTTTCAAAGGCATTACATCTCAAGGTCCAGGAACTTGACTTGGATCTGTTTCTCCGTGAAAAAATCTTATTGCCGCTTAGGCTTTCCACGTGTGAGTCACTCGTGGTCTTAAAATTGCGGGGCAGAATTCAACCAACACTCAATTCTTCTTTTCATGTATATTTACCGTCACTAAAGATTCTTCATCTCCATGAAACTATGATGTATTCTATCTTTGATGATGGTATGGAGTATGGTCTCAATAATTTCCTATCTGGTTGTCGACATCTGGAGGAACTTCTTTTAGATGAGAGCTTTCGAATACCCATCAACATCTCTTTTCACTTATTGAAGAGATTATTCCTTTCTTTATGTATGCCTACTTCTGTTATTAAGTGTTGTCCACTACAAATTAACGCCCCGTCACTCGAAGTTCTTTCTATTACTGATTTTTCATTGAAGCCTAGAAAATATGAGTTTGCAAGCTTGTCCAATCTTGATCGAGCTACTCTTTCCATTTGTAAGCGTCCGGATTTTAACAATTTGTATACACTCTTGGAAGGACTTTCTAATGTCAAATCATTGGCTCTCGGTTCCAATACTTATCATGTAAGTTTCATAAACTCTTTCCTTTTTTAATTACAAGTTCCATATGTAGATGCAAGTCTATTCTCATTTTATTATTTTGTTATGCAGTTTCTCAGCATGAAAGACAAACTTGACAACTTACATCTTCTCACTTTTCACAATTTGTTATTTTTATCGATTGAAATATCTGAGAGTTGCAGTTGGAATATGCTCATCAGCTTTCTGCTGAATGCTCCAAAACTTAAGGATCTTGCAATTGAGGTGAGTGTGATGTTTTTTTTAACAATATTTGCACAATACTTTTTTAAAAGATAAAATATAATAAGCTCAAAATCTAGCACAGGAAGAGCAAAGGCTTAGCAAAAGATACATGAAGTTTGCAAATAAAACCGATAAACAAATTAATTAATTCAAAATAATAGATATTTGTGTAGATTTTCCTACCAATTTAACCTTCTTTTAAACTATTTTAATTCCAATTTTTATCTTCATTTACATAATAATATAATAGAAACTATTTACTATATATCAGTTGGAAAATTCTAATGATAATTTTTGGTTTACAGAGAAAAATTGAAATCAATTCCCGAAGAAAGGAGGTTGGAAACTCGAGTTGGGTGGAGCCATCAGTAACTCCGGCATGTCTTACAAGCAGTCTCATTACATTTGAATTTAAAGGAATTCAAAACATAAAAACTGAGTTGGACTTTACACGACATATTGTAAGTCACTCAAGTAAGTTAGCGAAGGTAAAAATTTTCACACCTAGTTCAAAGAAAAGAAGAGTAGAAAAAAGTTTACGCCAGGGGTCAAAGAAATCCTCCGTACTAGTATGGGTATGTAATTTACACGCTTTCTTTTCTTCCACACAGAGTTTATATACTTTCTTTTTTAAAGAAAAAAGTTGGCATGTTAATATTTGTTGGTATGATGTTGTGGGGTGGTGATAGAACTCTCATCCTCTCTTATTGTTCTAGACATTAACTCTGACCACACACACACACACACACACTGACCCCACACACACACAGACACACACACGCGCGCACACACACGCGCACACACACACACACACACACTATTACATTCGTTGTTGAGAATAAGACTTTTTCTTAAAATAAATAATTTATATCCTCTTAAATTTTTTTAATTATATTAATATTTTTATCGGTACACTCTAATGATTTTATCATTAATGATTATAATCTTAAAATTAGTTATTCAATCTTGGTTACAGGATATTAATTCAATTTGAAGATCATTTGGATTCGTTCATGGATTCAATCAAGACCACTCGAGACTTTTAA
Protein sequence

>MS.gene03758.t1

MNMMVTYMFIHMLQMVRLMVQLTNGSQSAHKIPNLHFQLPEISDPVLSKEEFQSLNLDVIRRNENIKKLRLDSEKGCQQHDVHMWVSKAFVNFALLRRTENIRKLRLDSDKGCQPHDVHMWVSKALHLKVQELDLDLFLREKILLPLRLSTCESLVVLKLRGRIQPTLNSSFHVYLPSLKILHLHETMMYSIFDDGMEYGLNNFLSGCRHLEELLLDESFRIPINISFHLLKRLFLSLCMPTSVIKCCPLQINAPSLEVLSITDFSLKPRKYEFASLSNLDRATLSICKRPDFNNLYTLLEGLSNVKSLALGSNTYHFLSMKDKLDNLHLLTFHNLLFLSIEISESCSWNMLISFLLNAPKLKDLAIERKIEINSRRKEVGNSSWVEPSVTPACLTSSLITFEFKGIQNIKTELDFTRHIVSHSSKLAKVKIFTPSSKKRRVEKSLRQGSKKSSVLVWLFNLGYRILIQFEDHLDSFMDSIKTTRDF