AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89957

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89957.t1 MTR_4g056040 56.316 380 68 8 1 282 1 380 3.99e-124 365
MS.gene89957.t1 MTR_4g056020 53.714 350 94 8 1 282 1 350 1.53e-108 323
MS.gene89957.t1 MTR_4g055990 78.698 169 29 2 1 162 1 169 4.56e-87 270
MS.gene89957.t1 MTR_4g055990 72.034 118 33 0 161 278 266 383 4.76e-52 179
MS.gene89957.t1 MTR_4g055990 78.698 169 29 2 1 162 1 169 6.79e-87 270
MS.gene89957.t1 MTR_4g055990 72.034 118 33 0 161 278 266 383 7.92e-52 178
MS.gene89957.t1 MTR_4g055580 78.698 169 29 2 1 162 92 260 3.37e-86 270
MS.gene89957.t1 MTR_4g056070 79.070 172 26 3 1 162 1 172 5.02e-86 265
MS.gene89957.t1 MTR_4g056070 79.508 122 25 0 161 282 269 390 8.68e-59 195
MS.gene89957.t1 MTR_4g056030 60.714 168 24 2 1 162 1 132 2.13e-55 187
MS.gene89957.t1 MTR_4g056030 71.311 122 35 0 161 282 229 350 4.40e-52 178
MS.gene89957.t1 MTR_7g110920 66.935 124 41 0 156 279 93 216 9.62e-50 168
MS.gene89957.t1 MTR_1g038710 68.644 118 37 0 156 273 240 357 4.19e-48 167
MS.gene89957.t1 MTR_1g038710 47.059 238 71 6 1 222 11 209 3.92e-44 157
MS.gene89957.t1 MTR_1g090883 63.235 136 49 1 144 279 223 357 1.98e-46 161
MS.gene89957.t1 MTR_1g090883 47.879 165 43 3 2 159 12 140 4.36e-35 131
MS.gene89957.t1 MTR_1g090900 50.000 168 39 4 1 159 1 132 1.29e-39 138
MS.gene89957.t1 MTR_4g056010 89.231 65 7 0 98 162 1 65 2.06e-33 119
MS.gene89957.t1 MTR_4g093690 34.432 273 100 9 1 200 10 276 4.01e-31 118
MS.gene89957.t1 MTR_4g056020 82.000 50 9 0 229 278 1 50 3.40e-21 89.0
MS.gene89957.t1 MTR_6g022390 42.424 66 38 0 9 74 5 70 1.39e-11 62.0
MS.gene89957.t1 MTR_2g007100 59.091 44 18 0 9 52 2 45 2.03e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89957.t1 AT3G59000 27.941 272 136 8 9 279 2 214 1.70e-16 79.3
MS.gene89957.t1 AT3G03030 25.000 372 168 13 9 279 2 363 3.18e-14 73.6
MS.gene89957.t1 AT3G59190 25.263 285 174 9 9 289 12 261 3.29e-13 70.1
MS.gene89957.t1 AT3G59190 25.263 285 174 9 9 289 12 261 5.17e-13 69.7
MS.gene89957.t1 AT3G03030 33.766 154 74 4 9 158 2 131 5.81e-13 68.9
MS.gene89957.t1 AT1G58310 27.638 199 100 6 8 192 7 175 9.11e-13 68.9
MS.gene89957.t1 AT5G38392 34.868 152 69 4 9 154 2 129 1.04e-12 66.6
MS.gene89957.t1 AT3G59000 34.104 173 89 5 9 177 2 153 1.49e-12 68.6
MS.gene89957.t1 AT3G03040 33.113 151 79 2 9 158 2 131 1.57e-12 68.2
MS.gene89957.t1 AT5G38570 27.917 240 124 6 9 240 2 200 3.67e-11 63.9
MS.gene89957.t1 AT4G14103 31.579 152 72 4 8 155 7 130 6.41e-11 63.2
MS.gene89957.t1 AT3G59230 30.921 152 81 4 9 158 12 141 7.43e-11 63.2
MS.gene89957.t1 AT3G62440 60.000 45 18 0 9 53 2 46 7.73e-11 63.2
MS.gene89957.t1 AT4G14096 28.947 152 76 4 8 155 7 130 8.78e-11 63.2
MS.gene89957.t1 AT4G14096 28.947 152 76 4 8 155 7 130 8.78e-11 63.2
MS.gene89957.t1 AT5G38386 23.692 325 167 9 9 279 2 299 9.54e-11 62.8
MS.gene89957.t1 AT5G38386 23.692 325 167 9 9 279 2 299 9.54e-11 62.8

Find 61 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTACGTATCTTGCAATTA+AGG 0.212200 4.2:+47702830 MS.gene89957:CDS
ATTGCAAGATACGTAAGTTT+TGG 0.274598 4.2:-47702825 None:intergenic
GCAGATCGACTCCCATCTTT+TGG 0.302315 4.2:-47701740 None:intergenic
TTTCAATTACCAGAGATTTC+AGG 0.309929 4.2:+47701784 MS.gene89957:CDS
TCTCCGCAGTGGACAAAGTT+AGG 0.357588 4.2:-47704133 None:intergenic
ACCTATTGGTCATCCACCTT+CGG 0.375784 4.2:+47704177 MS.gene89957:CDS
ACTTTCTAATGTCGAATCAT+TGG 0.389882 4.2:+47702560 MS.gene89957:CDS
ATATGACAAATCAAATCGTC+TGG 0.414750 4.2:-47701670 None:intergenic
AAAGTGAAGGTTGGGAATCT+TGG 0.417455 4.2:-47701764 None:intergenic
CCTATTGGTCATCCACCTTC+GGG 0.417624 4.2:+47704178 MS.gene89957:CDS
TAGAAAGAACTTCAAGTGTC+GGG 0.418139 4.2:-47702416 None:intergenic
CAAGGTCCAGGAACTTGACT+TGG 0.426522 4.2:+47702038 MS.gene89957:CDS
TCTTTCCATTTGTCAGAGTC+CGG 0.430288 4.2:+47702506 MS.gene89957:CDS
AAATCCCACATCAAACCTAT+TGG 0.433951 4.2:+47704163 MS.gene89957:CDS
CAAAATCTTGATAGTGAGAA+AGG 0.438135 4.2:+47701859 MS.gene89957:CDS
CCCGAAGGTGGATGACCAAT+AGG 0.439721 4.2:-47704178 None:intergenic
GGTAATTGAAAGTGAAGGTT+GGG 0.443422 4.2:-47701772 None:intergenic
ACAAATCCAAGTCAAGTTCC+TGG 0.445726 4.2:-47702044 None:intergenic
CTAAGGCTCGAGAGCGAGAA+AGG 0.450221 4.2:+47701967 MS.gene89957:CDS
ACCCACATGTGTACATCATT+TGG 0.460055 4.2:-47701997 None:intergenic
CATGTGTTCTATCCAAAAGA+TGG 0.467948 4.2:+47701728 MS.gene89957:CDS
TGATTTGTTCGAACAACAAA+GGG 0.478379 4.2:-47701807 None:intergenic
AACAAAGGGCCTGAAATCTC+TGG 0.488546 4.2:-47701793 None:intergenic
GGCATTAGATCTCAAGGTCC+AGG 0.496808 4.2:+47702026 MS.gene89957:CDS
TGTTCACATGTGGGTTTCAA+AGG 0.497154 4.2:+47701897 MS.gene89957:CDS
GAATACAAACTGTTAAAATC+CGG 0.506388 4.2:-47702525 None:intergenic
AAAATCCGGACTCTGACAAA+TGG 0.507415 4.2:-47702511 None:intergenic
GTTGTCAAGTTTGTCCTCCA+TGG 0.514651 4.2:-47702706 None:intergenic
GAAACTTACATGAAATGTGT+TGG 0.516194 4.2:-47702592 None:intergenic
AGTTTGTATTCACTCTTGAA+AGG 0.523785 4.2:+47702537 MS.gene89957:CDS
TGTACACATGTGGGTTTCAA+AGG 0.528762 4.2:+47702005 MS.gene89957:CDS
CTTGATCGTCAGCTGAGGTA+AGG 0.535048 4.2:-47704110 None:intergenic
CCGAAAATATAAGAAAACTA+AGG 0.538047 4.2:+47701950 MS.gene89957:CDS
TTCAAAGGCATTAGATCTCA+AGG 0.542872 4.2:+47702020 MS.gene89957:CDS
ATAGAAAGAACTTCAAGTGT+CGG 0.549697 4.2:-47702417 None:intergenic
TGGTAATTGAAAGTGAAGGT+TGG 0.551378 4.2:-47701773 None:intergenic
AAGAGTAGCTTGATGAACAT+TGG 0.552393 4.2:-47702487 None:intergenic
TTGATTTGTTCGAACAACAA+AGG 0.557384 4.2:-47701808 None:intergenic
TATCAAAGATTGCCCGAAGG+TGG 0.561256 4.2:-47704190 None:intergenic
ATGTGTTCTATCCAAAAGAT+GGG 0.563690 4.2:+47701729 MS.gene89957:CDS
AATGTCGAATCATTGGCTCT+CGG 0.584448 4.2:+47702567 MS.gene89957:CDS
ACATGTGTACATCATTTGGC+TGG 0.590853 4.2:-47701993 None:intergenic
ATTTATCACGTTCTCCGCAG+TGG 0.596211 4.2:-47704144 None:intergenic
AAATATCTGAGAGTTGCAGT+TGG 0.597797 4.2:+47702772 MS.gene89957:CDS
TATGACAAATCAAATCGTCT+GGG 0.606583 4.2:-47701669 None:intergenic
ACCACATGATGTTCACATGT+GGG 0.606890 4.2:+47701888 MS.gene89957:CDS
GCCAAATGATGTACACATGT+GGG 0.612968 4.2:+47701996 MS.gene89957:CDS
GGAAAGCCTAAGCGGCAACA+AGG 0.615818 4.2:-47702082 None:intergenic
TGTCATGCAGTTTCTCACCA+TGG 0.615958 4.2:+47702689 MS.gene89957:intron
GATGACCAATAGGTTTGATG+TGG 0.636422 4.2:-47704168 None:intergenic
AGAAAGAACTTCAAGTGTCG+GGG 0.643549 4.2:-47702415 None:intergenic
AACCACATGATGTTCACATG+TGG 0.644144 4.2:+47701887 MS.gene89957:CDS
TTAGGCTTGATCGTCAGCTG+AGG 0.661870 4.2:-47704115 None:intergenic
AGCCAAATGATGTACACATG+TGG 0.670458 4.2:+47701995 MS.gene89957:CDS
TCACAAGTGGAAAGCCTAAG+CGG 0.683053 4.2:-47702090 None:intergenic
ATGACCAATAGGTTTGATGT+GGG 0.683243 4.2:-47704167 None:intergenic
ACCCACATGTGAACATCATG+TGG 0.693828 4.2:-47701889 None:intergenic
TCTCTGGTAATTGAAAGTGA+AGG 0.696256 4.2:-47701777 None:intergenic
GGATATCAAAGATTGCCCGA+AGG 0.703963 4.2:-47704193 None:intergenic
CATGCAGTTTCTCACCATGG+AGG 0.721841 4.2:+47702692 MS.gene89957:intron
AAGCCTAACTTTGTCCACTG+CGG 0.752393 4.2:+47704130 MS.gene89957:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTCATTAATTTAAATTAAT+AGG + chr4.2:47704072-47704091 MS.gene89957:intron 10.0%
!! TTAATTTAAATTAATAGGAA+TGG + chr4.2:47704077-47704096 MS.gene89957:intron 10.0%
!!! CTATTAATTAATTTGTTTAT+CGG - chr4.2:47702983-47703002 None:intergenic 10.0%
!! AAATAGGAAAAAATAAAGAA+AGG - chr4.2:47702362-47702381 None:intergenic 15.0%
!! AGATAAAAGGTTAATATTAT+CGG - chr4.2:47703844-47703863 None:intergenic 15.0%
!! AGTACATATTATATAATGAT+AGG + chr4.2:47703758-47703777 MS.gene89957:intron 15.0%
!!! TCACAGTTTATTATTTTTAT+CGG + chr4.2:47702746-47702765 MS.gene89957:CDS 15.0%
!!! TTCTTTTCTAATAAAAAAGT+TGG + chr4.2:47703473-47703492 MS.gene89957:intron 15.0%
!!! TTTGTTTTCTTATATATTCT+TGG - chr4.2:47701843-47701862 None:intergenic 15.0%
!! ATAGTTACAATGTAGATAAA+AGG - chr4.2:47703857-47703876 None:intergenic 20.0%
!! ATGAATGGAATTATTAAAGA+AGG + chr4.2:47704045-47704064 MS.gene89957:intron 20.0%
!! CTAGTTAATAATTAACTTAG+CGG + chr4.2:47703815-47703834 MS.gene89957:intron 20.0%
!! CTCATTACATTTGAATTTAT+TGG + chr4.2:47703245-47703264 MS.gene89957:intron 20.0%
!! GTAAATAAACATAAAACTGT+AGG - chr4.2:47703077-47703096 None:intergenic 20.0%
!! TTAAGAACAGAATTATATCT+GGG - chr4.2:47702659-47702678 None:intergenic 20.0%
!!! ATTTGTATCTTTTAACCATT+TGG + chr4.2:47703645-47703664 MS.gene89957:intron 20.0%
!!! CAAATTTTAGAAAGAGAAAT+AGG - chr4.2:47702378-47702397 None:intergenic 20.0%
!!! CCTTAGTTTTCTTATATTTT+CGG - chr4.2:47701953-47701972 None:intergenic 20.0%
! AAATTCAAATGTAATGAGAC+TGG - chr4.2:47703243-47703262 None:intergenic 25.0%
! AAGAATATATCACAGTTGAT+CGG - chr4.2:47702209-47702228 None:intergenic 25.0%
! AAGTCTTTATATGACATAAG+TGG + chr4.2:47703617-47703636 MS.gene89957:intron 25.0%
! AATATGATAAGCTCAAAATC+TGG + chr4.2:47702906-47702925 MS.gene89957:intron 25.0%
! AATATGTACTAACACTGAAT+GGG - chr4.2:47703748-47703767 None:intergenic 25.0%
! ACTATATATCCGATAATGTT+TGG + chr4.2:47703121-47703140 MS.gene89957:intron 25.0%
! ACTTCCAATATAACTTCATA+TGG + chr4.2:47703996-47704015 MS.gene89957:intron 25.0%
! AGCTTAAAAGAAGGTTAAAT+TGG - chr4.2:47703048-47703067 None:intergenic 25.0%
! CCGAAAATATAAGAAAACTA+AGG + chr4.2:47701950-47701969 MS.gene89957:CDS 25.0%
! CTTAAGAACAGAATTATATC+TGG - chr4.2:47702660-47702679 None:intergenic 25.0%
! GAATACAAACTGTTAAAATC+CGG - chr4.2:47702528-47702547 None:intergenic 25.0%
! TAAAAGAAGGTTAAATTGGT+AGG - chr4.2:47703044-47703063 None:intergenic 25.0%
! TAATATGTACTAACACTGAA+TGG - chr4.2:47703749-47703768 None:intergenic 25.0%
! TTTGCCATATGAAGTTATAT+TGG - chr4.2:47704003-47704022 None:intergenic 25.0%
!! GAAAGTATATAAACTCTATG+TGG - chr4.2:47703456-47703475 None:intergenic 25.0%
!! GATCTCAATAATTTTCTATC+TGG + chr4.2:47702252-47702271 MS.gene89957:intron 25.0%
!! TTTGCAAACTCATATTTTCT+AGG - chr4.2:47702462-47702481 None:intergenic 25.0%
!!! TTTATTGATTTCTAGCTTCT+GGG - chr4.2:47703599-47703618 None:intergenic 25.0%
AATGACTAGAACAATAAGAG+AGG - chr4.2:47703550-47703569 None:intergenic 30.0%
ACTTACGTATCTTGCAATTA+AGG + chr4.2:47702830-47702849 MS.gene89957:CDS 30.0%
ACTTTCTAATGTCGAATCAT+TGG + chr4.2:47702560-47702579 MS.gene89957:CDS 30.0%
AGATGAAGAATCTTTAGTGA+CGG - chr4.2:47702186-47702205 None:intergenic 30.0%
AGGTAAAACAGCTTAAAAGA+AGG - chr4.2:47703057-47703076 None:intergenic 30.0%
AGTTTGTATTCACTCTTGAA+AGG + chr4.2:47702537-47702556 MS.gene89957:CDS 30.0%
ATAGAAAGAACTTCAAGTGT+CGG - chr4.2:47702420-47702439 None:intergenic 30.0%
ATATGACAAATCAAATCGTC+TGG - chr4.2:47701673-47701692 None:intergenic 30.0%
ATGTGTTCTATCCAAAAGAT+GGG + chr4.2:47701729-47701748 MS.gene89957:CDS 30.0%
ATTTCATACCCATACTAGTA+CGG - chr4.2:47703414-47703433 None:intergenic 30.0%
GAAACTTACATGAAATGTGT+TGG - chr4.2:47702595-47702614 None:intergenic 30.0%
GAAATGGATACACTACTATT+TGG + chr4.2:47703925-47703944 MS.gene89957:intron 30.0%
GCATGTAAACCAAACATTAT+CGG - chr4.2:47703133-47703152 None:intergenic 30.0%
TATATCTGGGTGACAAAATA+TGG - chr4.2:47702646-47702665 None:intergenic 30.0%
TATGACAAATCAAATCGTCT+GGG - chr4.2:47701672-47701691 None:intergenic 30.0%
TCAAGACATAAAAACTGAGT+TGG + chr4.2:47703271-47703290 MS.gene89957:intron 30.0%
TCACTCAAATAAGTTAGAGA+AGG + chr4.2:47703316-47703335 MS.gene89957:intron 30.0%
TGAAAAGAAGAATTGAGTGT+TGG - chr4.2:47702153-47702172 None:intergenic 30.0%
TTATATTGGAAGTTTGATGC+AGG - chr4.2:47703989-47704008 None:intergenic 30.0%
TTTCAATTACCAGAGATTTC+AGG + chr4.2:47701784-47701803 MS.gene89957:CDS 30.0%
! ATTGCAAGATACGTAAGTTT+TGG - chr4.2:47702828-47702847 None:intergenic 30.0%
! CATATCGTTGTGATAATGAA+TGG + chr4.2:47704030-47704049 MS.gene89957:intron 30.0%
! GATTTGACTTTATTGATGGT+AGG - chr4.2:47703577-47703596 None:intergenic 30.0%
! GCTAATATTTGTTGGTATGA+TGG + chr4.2:47703499-47703518 MS.gene89957:intron 30.0%
! GTATCTTTTAACCATTTGGT+TGG + chr4.2:47703649-47703668 MS.gene89957:intron 30.0%
! TACTCTTCTTTTCTTTAAGC+TGG - chr4.2:47703357-47703376 None:intergenic 30.0%
!! CAAAATCTTGATAGTGAGAA+AGG + chr4.2:47701859-47701878 MS.gene89957:CDS 30.0%
!! CTTTATTGATTTCTAGCTTC+TGG - chr4.2:47703600-47703619 None:intergenic 30.0%
!! TGATTTGTTCGAACAACAAA+GGG - chr4.2:47701810-47701829 None:intergenic 30.0%
!! TTGATTTGTTCGAACAACAA+AGG - chr4.2:47701811-47701830 None:intergenic 30.0%
AAATAAAGTTCCTCCAGATG+TGG - chr4.2:47702282-47702301 None:intergenic 35.0%
AAATATCTGAGAGTTGCAGT+TGG + chr4.2:47702772-47702791 MS.gene89957:CDS 35.0%
AAATCCCACATCAAACCTAT+TGG + chr4.2:47704163-47704182 MS.gene89957:CDS 35.0%
AAGAGTAGCTTGATGAACAT+TGG - chr4.2:47702490-47702509 None:intergenic 35.0%
ACAAACAACATCCAACCAAA+TGG - chr4.2:47703663-47703682 None:intergenic 35.0%
AGTAGAAAAAAGCTTACGCA+AGG + chr4.2:47703373-47703392 MS.gene89957:intron 35.0%
ATGACCAATAGGTTTGATGT+GGG - chr4.2:47704170-47704189 None:intergenic 35.0%
CATGTGTTCTATCCAAAAGA+TGG + chr4.2:47701728-47701747 MS.gene89957:CDS 35.0%
GGTAATTGAAAGTGAAGGTT+GGG - chr4.2:47701775-47701794 None:intergenic 35.0%
GTAGAAAAAAGCTTACGCAA+GGG + chr4.2:47703374-47703393 MS.gene89957:intron 35.0%
GTTGGCATGCTAATATTTGT+TGG + chr4.2:47703491-47703510 MS.gene89957:intron 35.0%
TAGAAAAAAGCTTACGCAAG+GGG + chr4.2:47703375-47703394 MS.gene89957:intron 35.0%
TAGAAAGAACTTCAAGTGTC+GGG - chr4.2:47702419-47702438 None:intergenic 35.0%
TCTCTGGTAATTGAAAGTGA+AGG - chr4.2:47701780-47701799 None:intergenic 35.0%
TCTCTTCAAGAAGTGAAAAG+AGG - chr4.2:47702334-47702353 None:intergenic 35.0%
TGAAATCAATTCCCGAAGAA+AGG + chr4.2:47703163-47703182 MS.gene89957:intron 35.0%
TGGTAATTGAAAGTGAAGGT+TGG - chr4.2:47701776-47701795 None:intergenic 35.0%
! ACTTGTGGTCTTAAAATTGC+GGG + chr4.2:47702116-47702135 MS.gene89957:intron 35.0%
! ATTTGTTGGTATGATGGTGT+GGG + chr4.2:47703505-47703524 MS.gene89957:intron 35.0%
! CACTTGTGGTCTTAAAATTG+CGG + chr4.2:47702115-47702134 MS.gene89957:intron 35.0%
! CTGGGATTTGACTTTATTGA+TGG - chr4.2:47703581-47703600 None:intergenic 35.0%
! TATTTGTTGGTATGATGGTG+TGG + chr4.2:47703504-47703523 MS.gene89957:intron 35.0%
! TGCAGAGTTACTCTTTTAGA+AGG - chr4.2:47703896-47703915 None:intergenic 35.0%
!! GAAGTGAAAAGAGGTATTGA+TGG - chr4.2:47702325-47702344 None:intergenic 35.0%
!! TTCAAAGGCATTAGATCTCA+AGG + chr4.2:47702020-47702039 MS.gene89957:CDS 35.0%
AAAATCCGGACTCTGACAAA+TGG - chr4.2:47702514-47702533 None:intergenic 40.0%
AAAGTGAAGGTTGGGAATCT+TGG - chr4.2:47701767-47701786 None:intergenic 40.0%
AAATCACTCTCTTAGCAGGT+TGG - chr4.2:47703724-47703743 None:intergenic 40.0%
AACCACATGATGTTCACATG+TGG + chr4.2:47701887-47701906 MS.gene89957:CDS 40.0%
AATCAATTCCCGAAGAAAGG+AGG + chr4.2:47703166-47703185 MS.gene89957:intron 40.0%
ACAAATCCAAGTCAAGTTCC+TGG - chr4.2:47702047-47702066 None:intergenic 40.0%
ACATGTGTACATCATTTGGC+TGG - chr4.2:47701996-47702015 None:intergenic 40.0%
ACCACATGATGTTCACATGT+GGG + chr4.2:47701888-47701907 MS.gene89957:CDS 40.0%
ACCCACATGTGTACATCATT+TGG - chr4.2:47702000-47702019 None:intergenic 40.0%
AGAAATCCTCCGTACTAGTA+TGG + chr4.2:47703402-47703421 MS.gene89957:intron 40.0%
AGCCAAATGATGTACACATG+TGG + chr4.2:47701995-47702014 MS.gene89957:CDS 40.0%
AGGCAACATGTATCAGCAAT+TGG - chr4.2:47703956-47703975 None:intergenic 40.0%
AGTTTCCAACCTCCTTTCTT+CGG - chr4.2:47703178-47703197 None:intergenic 40.0%
GAAATCCTCCGTACTAGTAT+GGG + chr4.2:47703403-47703422 MS.gene89957:intron 40.0%
GAAGTTTGATGCAGGAGAAA+TGG - chr4.2:47703981-47704000 None:intergenic 40.0%
GATGACCAATAGGTTTGATG+TGG - chr4.2:47704171-47704190 None:intergenic 40.0%
GCCAAATGATGTACACATGT+GGG + chr4.2:47701996-47702015 MS.gene89957:CDS 40.0%
TCTTTCCATTTGTCAGAGTC+CGG + chr4.2:47702506-47702525 MS.gene89957:CDS 40.0%
TGTACACATGTGGGTTTCAA+AGG + chr4.2:47702005-47702024 MS.gene89957:CDS 40.0%
TGTTCACATGTGGGTTTCAA+AGG + chr4.2:47701897-47701916 MS.gene89957:CDS 40.0%
TTGATGCAGGAGAAATGGAA+AGG - chr4.2:47703976-47703995 None:intergenic 40.0%
TTGTTTGTTCGATCCTTGGT+CGG + chr4.2:47703675-47703694 MS.gene89957:intron 40.0%
! AAAAAACCTTGTTGCCGCTT+AGG + chr4.2:47702076-47702095 MS.gene89957:CDS 40.0%
! AGAAAGAACTTCAAGTGTCG+GGG - chr4.2:47702418-47702437 None:intergenic 40.0%
! AGACTGGTTATAAGACATGC+TGG - chr4.2:47703227-47703246 None:intergenic 40.0%
! CTTGTGGTCTTAAAATTGCG+GGG + chr4.2:47702117-47702136 MS.gene89957:intron 40.0%
! GATGTTGTTTGTTCGATCCT+TGG + chr4.2:47703671-47703690 MS.gene89957:intron 40.0%
! GTCGGGGTATTAATTTCTAC+TGG - chr4.2:47702402-47702421 None:intergenic 40.0%
! TTGTGGTCTTAAAATTGCGG+GGG + chr4.2:47702118-47702137 MS.gene89957:intron 40.0%
! TTTGTTGGTATGATGGTGTG+GGG + chr4.2:47703506-47703525 MS.gene89957:intron 40.0%
!! AATGTCGAATCATTGGCTCT+CGG + chr4.2:47702567-47702586 MS.gene89957:CDS 40.0%
!! GGTATTGATGGCTATTCGAA+AGG - chr4.2:47702313-47702332 None:intergenic 40.0%
AACAAAGGGCCTGAAATCTC+TGG - chr4.2:47701796-47701815 None:intergenic 45.0%
AAGCCTAACTTTGTCCACTG+CGG + chr4.2:47704130-47704149 MS.gene89957:CDS 45.0%
AATTCCCGAAGAAAGGAGGT+TGG + chr4.2:47703170-47703189 MS.gene89957:intron 45.0%
ACCCACATGTGAACATCATG+TGG - chr4.2:47701892-47701911 None:intergenic 45.0%
ACCTATTGGTCATCCACCTT+CGG + chr4.2:47704177-47704196 MS.gene89957:CDS 45.0%
ATTTATCACGTTCTCCGCAG+TGG - chr4.2:47704147-47704166 None:intergenic 45.0%
GCACAAGAAGAGCAAAGACT+TGG + chr4.2:47702928-47702947 MS.gene89957:intron 45.0%
GGGAAAATCACTCTCTTAGC+AGG - chr4.2:47703728-47703747 None:intergenic 45.0%
GTTGTCAAGTTTGTCCTCCA+TGG - chr4.2:47702709-47702728 None:intergenic 45.0%
GTTTCCAACCTCCTTTCTTC+GGG - chr4.2:47703177-47703196 None:intergenic 45.0%
TCACAAGTGGAAAGCCTAAG+CGG - chr4.2:47702093-47702112 None:intergenic 45.0%
TCATACCCATACTAGTACGG+AGG - chr4.2:47703411-47703430 None:intergenic 45.0%
TCTATCTGGTTGTCCACATC+TGG + chr4.2:47702266-47702285 MS.gene89957:intron 45.0%
TGTCATGCAGTTTCTCACCA+TGG + chr4.2:47702689-47702708 MS.gene89957:intron 45.0%
TTCCACTTGTGAGTCACTTG+TGG + chr4.2:47702101-47702120 MS.gene89957:intron 45.0%
!!! GCGGCAACAAGGTTTTTTCA+TGG - chr4.2:47702074-47702093 None:intergenic 45.0%
AGACATGCTGGAGTCACTGA+TGG - chr4.2:47703215-47703234 None:intergenic 50.0%
AGGAGGTTGGAAACTCAAGC+TGG + chr4.2:47703183-47703202 MS.gene89957:intron 50.0%
ATCTGGTTGTCCACATCTGG+AGG + chr4.2:47702269-47702288 MS.gene89957:intron 50.0%
CAAGGTCCAGGAACTTGACT+TGG + chr4.2:47702038-47702057 MS.gene89957:CDS 50.0%
CATGCAGTTTCTCACCATGG+AGG + chr4.2:47702692-47702711 MS.gene89957:intron 50.0%
CCTATTGGTCATCCACCTTC+GGG + chr4.2:47704178-47704197 MS.gene89957:CDS 50.0%
CTCTGCAGTGCACACAGAAA+TGG + chr4.2:47703909-47703928 MS.gene89957:intron 50.0%
CTTGATCGTCAGCTGAGGTA+AGG - chr4.2:47704113-47704132 None:intergenic 50.0%
GACCACAAGTGACTCACAAG+TGG - chr4.2:47702106-47702125 None:intergenic 50.0%
GGAGGTTGGAAACTCAAGCT+GGG + chr4.2:47703184-47703203 MS.gene89957:intron 50.0%
GGCATTAGATCTCAAGGTCC+AGG + chr4.2:47702026-47702045 MS.gene89957:CDS 50.0%
TCTCCGCAGTGGACAAAGTT+AGG - chr4.2:47704136-47704155 None:intergenic 50.0%
TGCTCAATAGACACCGACCA+AGG - chr4.2:47703691-47703710 None:intergenic 50.0%
TTAGGCTTGATCGTCAGCTG+AGG - chr4.2:47704118-47704137 None:intergenic 50.0%
!! GCAGATCGACTCCCATCTTT+TGG - chr4.2:47701743-47701762 None:intergenic 50.0%
CCCGAAGGTGGATGACCAAT+AGG - chr4.2:47704181-47704200 None:intergenic 55.0%
CTAAGGCTCGAGAGCGAGAA+AGG + chr4.2:47701967-47701986 MS.gene89957:CDS 55.0%
GGAAAGCCTAAGCGGCAACA+AGG - chr4.2:47702085-47702104 None:intergenic 55.0%
GGTTGGAAACTCAAGCTGGG+TGG + chr4.2:47703187-47703206 MS.gene89957:intron 55.0%
! GTTGGTATGATGGTGTGGGG+TGG + chr4.2:47703509-47703528 MS.gene89957:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 47701628 47704210 47701628 ID=MS.gene89957
chr4.2 mRNA 47701628 47704210 47701628 ID=MS.gene89957.t1;Parent=MS.gene89957
chr4.2 exon 47701628 47702108 47701628 ID=MS.gene89957.t1.exon1;Parent=MS.gene89957.t1
chr4.2 CDS 47701628 47702108 47701628 ID=cds.MS.gene89957.t1;Parent=MS.gene89957.t1
chr4.2 exon 47702403 47702605 47702403 ID=MS.gene89957.t1.exon2;Parent=MS.gene89957.t1
chr4.2 CDS 47702403 47702605 47702403 ID=cds.MS.gene89957.t1;Parent=MS.gene89957.t1
chr4.2 exon 47702699 47702851 47702699 ID=MS.gene89957.t1.exon3;Parent=MS.gene89957.t1
chr4.2 CDS 47702699 47702851 47702699 ID=cds.MS.gene89957.t1;Parent=MS.gene89957.t1
chr4.2 exon 47704094 47704210 47704094 ID=MS.gene89957.t1.exon4;Parent=MS.gene89957.t1
chr4.2 CDS 47704094 47704210 47704094 ID=cds.MS.gene89957.t1;Parent=MS.gene89957.t1
Gene Sequence

>MS.gene89957

ATGGCGTGCTCTGATGAAGAACACGATCGATTAAGCAATCTCCCAGACGATTTGATTTGTCATATTTTGTCTTTCATATCAACAAAAGAGTGTTATAGAACATGTGTTCTATCCAAAAGATGGGAGTCGATCTGCACCAAGATTCCCAACCTTCACTTTCAATTACCAGAGATTTCAGGCCCTTTGTTGTTCGAACAAATCAAGTCTGTTTACCAAGAATATATAAGAAAACAAAATCTTGATAGTGAGAAAGGTTGCGAACCACATGATGTTCACATGTGGGTTTCAAAGGCATTTGTAAATTTTGCTCTGCTAAGACGCACCGAAAATATAAGAAAACTAAGGCTCGAGAGCGAGAAAGGCTGCCAGCCAAATGATGTACACATGTGGGTTTCAAAGGCATTAGATCTCAAGGTCCAGGAACTTGACTTGGATTTGTTTCTCCATGAAAAAACCTTGTTGCCGCTTAGGCTTTCCACTTGTGAGTCACTTGTGGTCTTAAAATTGCGGGGGAGAATTCAACCAACACTCAATTCTTCTTTTCATGTATATTTACCGTCACTAAAGATTCTTCATCTCCGATCAACTGTGATATATTCTTTCTTTGATGATCAAAAATAGTATGATCTCAATAATTTTCTATCTGGTTGTCCACATCTGGAGGAACTTTATTTAAATGAGACCTTTCGAATAGCCATCAATACCTCTTTTCACTTCTTGAAGAGATTATACCTTTCTTTATTTTTTCCTATTTCTCTTTCTAAAATTTGTCCAGTAGAAATTAATACCCCGACACTTGAAGTTCTTTCTATTATTGATTTGTCATTGACTCCTAGAAAATATGAGTTTGCAAACTTGTCCAATGTTCATCAAGCTACTCTTTCCATTTGTCAGAGTCCGGATTTTAACAGTTTGTATTCACTCTTGAAAGGACTTTCTAATGTCGAATCATTGGCTCTCGGTTCCAACACATTTCATGTAAGTTTCATAATCTCTTCTTTTTTAATTACAAGTTCCATATTTTGTCACCCAGATATAATTCTGTTCTTAAGTTATTATTTTGTCATGCAGTTTCTCACCATGGAGGACAAACTTGACAACTTACATCTTCTCACTTTTCACAGTTTATTATTTTTATCGGTTGAAATATCTGAGAGTTGCAGTTGGAATATGCTCGTCAGCTTTCTGCAGAATGCTCCAAAACTTACGTATCTTGCAATTAAGGTGTGTATGATGTTTTTTTTAACTATATTTGCACAATATATTTTTAAAAGATAAAATATGATAAGCTCAAAATCTGGCACAAGAAGAGCAAAGACTTGGCAAAAGATACATGTAGTTTGCAAATATAACCGATAAACAAATTAATTAATAGAAAACAATAGATATCTGATCTATATTTATGTAGATTTTCCTACCAATTTAACCTTCTTTTAAGCTGTTTTACCTACAGTTTTATGTTTATTTACTTAATAATATAATTGAAACTATTTACTATATATCCGATAATGTTTGGTTTACATGCAGAGAAAACTTGAAATCAATTCCCGAAGAAAGGAGGTTGGAAACTCAAGCTGGGTGGAGCCATCAGTGACTCCAGCATGTCTTATAACCAGTCTCATTACATTTGAATTTATTGGAATTCAAGACATAAAAACTGAGTTGGACTTTACGCGATATATTGTAAGTCACTCAAATAAGTTAGAGAAGGTAAAGATTTTCACAGCCAGCTTAAAGAAAAGAAGAGTAGAAAAAAGCTTACGCAAGGGGTCAAAGAAATCCTCCGTACTAGTATGGGTATGAAATTTACACACTTTCTTTTCTTCCACATAGAGTTTATATACTTTCTTTTCTAATAAAAAAGTTGGCATGCTAATATTTGTTGGTATGATGGTGTGGGGTGGTGATAGAACTTTCATCCTCTCTTATTGTTCTAGTCATTAACTCCTACCATCAATAAAGTCAAATCCCAGAAGCTAGAAATCAATAAAGTCTTTATATGACATAAGTGGTAGATATTTGTATCTTTTAACCATTTGGTTGGATGTTGTTTGTTCGATCCTTGGTCGGTGTCTATTGAGCAGTCTTATCAACCAACCTGCTAAGAGAGTGATTTTCCCATTCAGTGTTAGTACATATTATATAATGATAGGACTACTACACAAACTAACTTGCTAGAGTCAATAACTAGTTAATAATTAACTTAGCGGAAACCGATAATATTAACCTTTTATCTACATTGTAACTATTTTATCTACATTATCACCTTCTAAAAGAGTAACTCTGCAGTGCACACAGAAATGGATACACTACTATTTGGTCACACCAATTGCTGATACATGTTGCCTTTCCATTTCTCCTGCATCAAACTTCCAATATAACTTCATATGGCAAAACAACGACATATCGTTGTGATAATGAATGGAATTATTAAAGAAGGTAAACTTCATTAATTTAAATTAATAGGAATGGATAGTTTCAACCTTACCTCAGCTGACGATCAAGCCTAACTTTGTCCACTGCGGAGAACGTGATAAATCCCACATCAAACCTATTGGTCATCCACCTTCGGGCAATCTTTGA
Protein sequence

>MS.gene89957.t1

MACSDEEHDRLSNLPDDLICHILSFISTKECYRTCVLSKRWESICTKIPNLHFQLPEISGPLLFEQIKSVYQEYIRKQNLDSEKGCEPHDVHMWVSKAFVNFALLRRTENIRKLRLESEKGCQPNDVHMWVSKALDLKVQELDLDLFLHEKTLLPLRLSTLEINTPTLEVLSIIDLSLTPRKYEFANLSNVHQATLSICQSPDFNSLYSLLKGLSNVESLALGSNTFHFLTMEDKLDNLHLLTFHSLLFLSVEISESCSWNMLVSFLQNAPKLTYLAIKEWIVSTLPQLTIKPNFVHCGERDKSHIKPIGHPPSGNL