AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09367

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09367.t1 MTR_4g055990 91.165 498 25 1 1 498 1 479 0.0 902
MS.gene09367.t1 MTR_4g055990 91.165 498 25 1 1 498 1 479 0.0 902
MS.gene09367.t1 MTR_4g055580 82.731 498 61 3 1 498 92 564 0.0 791
MS.gene09367.t1 MTR_4g056040 77.309 498 82 3 1 498 1 467 0.0 750
MS.gene09367.t1 MTR_4g056020 76.305 498 57 4 1 498 1 437 0.0 730
MS.gene09367.t1 MTR_4g056070 77.874 461 75 3 1 458 1 437 0.0 704
MS.gene09367.t1 MTR_4g056030 71.285 498 82 4 1 498 1 437 0.0 678
MS.gene09367.t1 MTR_1g038710 56.944 504 148 8 1 498 11 451 7.32e-169 484
MS.gene09367.t1 MTR_1g090883 54.854 412 126 5 2 411 12 365 4.79e-135 395
MS.gene09367.t1 MTR_7g110920 56.704 358 103 5 155 498 1 320 4.24e-122 360
MS.gene09367.t1 MTR_4g056020 82.192 146 21 1 353 498 1 141 1.49e-77 240
MS.gene09367.t1 MTR_4g093690 45.427 328 114 8 1 324 10 276 3.30e-67 218
MS.gene09367.t1 MTR_4g056010 90.000 100 10 0 124 223 1 100 1.35e-56 184
MS.gene09367.t1 MTR_1g090900 48.927 233 60 5 1 230 1 177 4.35e-50 170
MS.gene09367.t1 MTR_1g090890 63.333 90 27 2 412 498 30 116 2.81e-29 111
MS.gene09367.t1 MTR_5g080710 28.306 484 241 16 9 477 33 425 4.02e-24 105
MS.gene09367.t1 MTR_8g062270 27.158 475 269 16 9 472 16 424 1.41e-21 97.8
MS.gene09367.t1 MTR_8g014260 26.602 515 271 16 6 495 13 445 5.82e-21 95.9
MS.gene09367.t1 MTR_7g115270 26.392 485 245 17 9 490 12 387 8.20e-21 94.7
MS.gene09367.t1 MTR_8g058970 26.892 502 286 19 4 484 14 455 1.92e-20 94.4
MS.gene09367.t1 MTR_5g084070 29.345 351 214 11 132 477 78 399 5.93e-20 92.8
MS.gene09367.t1 MTR_8g468810 25.773 485 268 15 4 472 20 428 2.22e-18 88.2
MS.gene09367.t1 MTR_7g056433 26.261 476 218 18 4 472 7 356 1.16e-17 85.1
MS.gene09367.t1 MTR_8g467800 30.874 366 211 16 115 461 70 412 2.52e-17 84.7
MS.gene09367.t1 MTR_8g468840 25.413 484 266 20 8 472 13 420 9.89e-17 82.8
MS.gene09367.t1 MTR_8g445780 28.169 355 195 12 126 457 72 389 3.38e-16 80.9
MS.gene09367.t1 MTR_5g007410 25.216 464 229 18 11 472 28 375 8.89e-16 80.1
MS.gene09367.t1 MTR_2g083840 29.921 381 221 15 124 486 85 437 4.28e-15 77.8
MS.gene09367.t1 MTR_8g468810 26.761 355 183 11 4 346 20 309 1.23e-14 75.5
MS.gene09367.t1 MTR_8g468810 26.761 355 183 11 4 346 20 309 1.40e-14 75.5
MS.gene09367.t1 MTR_8g468810 26.761 355 183 11 4 346 20 309 1.43e-14 75.1
MS.gene09367.t1 MTR_4g080773 26.458 480 273 19 9 472 19 434 3.44e-14 75.1
MS.gene09367.t1 MTR_4g080773 26.458 480 273 19 9 472 19 434 3.60e-14 75.1
MS.gene09367.t1 MTR_8g468810 26.761 355 183 11 4 346 20 309 5.00e-14 73.9
MS.gene09367.t1 MTR_5g008950 32.432 111 65 2 367 474 233 336 6.01e-12 67.8
MS.gene09367.t1 MTR_6g044572 30.435 253 124 11 9 251 2 212 6.48e-12 67.8
MS.gene09367.t1 MTR_3g048290 25.714 490 266 19 5 472 19 432 1.47e-11 67.0
MS.gene09367.t1 MTR_6g044560 30.435 253 124 11 9 251 178 388 1.93e-11 66.6
MS.gene09367.t1 MTR_8g467800 33.333 252 138 10 115 350 70 307 2.22e-11 65.5
MS.gene09367.t1 MTR_7g110910 81.579 38 7 0 1 38 158 195 5.75e-11 62.4
MS.gene09367.t1 MTR_4g045680 41.584 101 53 3 6 102 13 111 7.80e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09367.t1 AT5G62970 29.938 481 259 21 9 476 2 417 1.45e-26 112
MS.gene09367.t1 AT4G00315 27.218 496 264 20 9 483 2 421 2.96e-24 105
MS.gene09367.t1 AT3G59160 26.241 423 237 13 9 401 13 390 1.03e-23 104
MS.gene09367.t1 AT5G38396 27.126 494 259 18 9 473 2 423 1.45e-23 103
MS.gene09367.t1 AT4G26340 27.368 475 243 21 9 471 2 386 8.91e-23 100
MS.gene09367.t1 AT4G10400 24.947 469 255 13 9 472 2 378 1.47e-22 100
MS.gene09367.t1 AT4G10400 24.947 469 255 13 9 472 2 378 1.47e-22 100
MS.gene09367.t1 AT4G10400 24.947 469 255 13 9 472 2 378 1.47e-22 100
MS.gene09367.t1 AT1G55660 26.446 484 261 19 9 473 53 460 2.28e-22 100
MS.gene09367.t1 AT3G59190 27.765 425 213 14 9 404 12 371 7.34e-22 97.8
MS.gene09367.t1 AT3G59190 26.600 500 256 18 9 463 12 445 1.96e-21 97.8
MS.gene09367.t1 AT1G58310 28.029 421 222 16 9 401 8 375 2.48e-21 97.4
MS.gene09367.t1 AT4G14103 27.143 420 203 14 9 400 8 352 2.62e-21 96.3
MS.gene09367.t1 AT4G14103 27.143 420 203 14 9 400 8 352 2.62e-21 96.3
MS.gene09367.t1 AT5G56380 26.681 476 241 17 9 460 2 393 3.24e-21 96.7
MS.gene09367.t1 AT5G56380 26.681 476 241 17 9 460 2 393 3.24e-21 96.7
MS.gene09367.t1 AT1G51370 27.347 490 248 18 7 481 17 413 8.26e-21 95.1
MS.gene09367.t1 AT4G14103 27.918 437 222 17 9 420 8 376 1.10e-20 95.1
MS.gene09367.t1 AT4G00320 26.950 423 227 13 3 401 7 371 1.30e-20 95.1
MS.gene09367.t1 AT3G50710 26.971 482 253 18 9 477 2 397 3.00e-20 93.6
MS.gene09367.t1 AT2G26860 29.674 337 206 9 149 476 86 400 3.63e-20 93.2
MS.gene09367.t1 AT2G26860 29.674 337 206 9 149 476 86 400 3.63e-20 93.2
MS.gene09367.t1 AT4G13965 27.010 485 248 21 9 480 10 401 1.11e-19 91.7
MS.gene09367.t1 AT4G14096 25.635 433 233 14 8 420 7 370 2.46e-19 90.9
MS.gene09367.t1 AT4G14096 25.635 433 233 14 8 420 7 370 2.46e-19 90.9
MS.gene09367.t1 AT5G41840 26.636 428 221 11 9 401 14 383 2.93e-19 91.3
MS.gene09367.t1 AT4G26350 24.286 490 251 16 9 473 2 396 4.52e-19 90.1
MS.gene09367.t1 AT5G41830 26.174 447 237 14 3 422 7 387 9.54e-19 89.4
MS.gene09367.t1 AT3G59170 27.064 436 220 16 9 401 7 387 1.75e-18 88.6
MS.gene09367.t1 AT3G59230 24.521 522 281 14 9 492 12 458 6.91e-18 86.7
MS.gene09367.t1 AT3G03030 26.450 431 229 12 9 409 2 374 7.99e-18 86.7
MS.gene09367.t1 AT1G32375 26.392 485 261 18 9 484 2 399 1.49e-17 85.1
MS.gene09367.t1 AT1G32375 26.392 485 261 18 9 484 2 399 1.49e-17 85.1
MS.gene09367.t1 AT3G59210 27.818 417 219 13 5 401 3 357 2.01e-17 85.1
MS.gene09367.t1 AT3G59210 27.818 417 219 13 5 401 3 357 2.01e-17 85.1
MS.gene09367.t1 AT3G59210 27.818 417 219 13 5 401 3 357 2.01e-17 85.1
MS.gene09367.t1 AT3G59210 27.818 417 219 13 5 401 3 357 2.01e-17 85.1
MS.gene09367.t1 AT3G59210 27.818 417 219 13 5 401 3 357 2.01e-17 85.1
MS.gene09367.t1 AT3G58880 25.328 458 256 13 9 446 3 394 2.07e-17 85.1
MS.gene09367.t1 AT4G10420 27.479 353 205 13 155 494 1 315 3.46e-17 83.2
MS.gene09367.t1 AT3G59200 25.000 432 217 12 8 404 6 365 4.20e-17 83.2
MS.gene09367.t1 AT5G56400 26.176 489 235 22 9 472 33 420 4.24e-17 84.0
MS.gene09367.t1 AT1G55660 27.479 353 178 13 9 346 53 342 5.80e-17 82.8
MS.gene09367.t1 AT3G52680 25.948 501 224 18 9 474 21 409 8.14e-17 83.2
MS.gene09367.t1 AT4G00160 28.033 478 245 20 9 474 16 406 8.70e-17 83.2
MS.gene09367.t1 AT3G26930 28.710 310 177 12 174 470 125 403 8.90e-17 83.2
MS.gene09367.t1 AT3G58920 25.768 423 226 13 9 402 2 365 1.02e-16 83.2
MS.gene09367.t1 AT3G26930 28.803 309 176 12 174 469 80 357 1.04e-16 82.4
MS.gene09367.t1 AT3G58860 25.586 469 258 16 6 456 4 399 1.16e-16 82.8
MS.gene09367.t1 AT3G59200 24.573 468 239 14 8 440 6 394 3.04e-16 81.6
MS.gene09367.t1 AT1G51370 27.594 453 221 17 7 445 17 376 3.12e-16 81.3
MS.gene09367.t1 AT5G60610 28.869 336 193 13 151 472 83 386 4.29e-16 80.5
MS.gene09367.t1 AT5G60610 28.869 336 193 13 151 472 83 386 4.29e-16 80.5
MS.gene09367.t1 AT3G59180 27.704 379 213 17 120 455 33 393 5.93e-16 80.9
MS.gene09367.t1 AT5G38590 28.150 373 217 16 113 474 53 385 7.37e-16 80.1
MS.gene09367.t1 AT1G06630 28.218 404 218 17 8 402 11 351 8.31e-16 79.7
MS.gene09367.t1 AT1G06630 28.218 404 218 17 8 402 11 351 8.31e-16 79.7
MS.gene09367.t1 AT1G06630 28.218 404 218 17 8 402 11 351 8.31e-16 79.7
MS.gene09367.t1 AT1G06630 28.218 404 218 17 8 402 11 351 8.31e-16 79.7
MS.gene09367.t1 AT3G49030 26.434 488 256 21 7 483 19 414 9.66e-16 79.7
MS.gene09367.t1 AT1G06630 28.218 404 218 17 8 402 20 360 1.31e-15 79.3
MS.gene09367.t1 AT1G52650 25.800 500 250 20 9 472 2 416 2.00e-15 79.0
MS.gene09367.t1 AT1G06630 28.218 404 218 17 8 402 11 351 2.22e-15 79.0
MS.gene09367.t1 AT1G51370 30.242 248 105 8 7 254 17 196 2.77e-15 77.8
MS.gene09367.t1 AT3G58900 26.555 418 222 10 9 402 2 358 2.91e-15 78.6
MS.gene09367.t1 AT5G56700 30.704 355 204 17 131 474 29 352 4.34e-15 77.0
MS.gene09367.t1 AT5G56410 26.477 491 281 21 9 486 2 425 4.56e-15 78.2
MS.gene09367.t1 AT4G13960 24.848 495 262 17 9 473 2 416 8.52e-15 77.0
MS.gene09367.t1 AT4G13960 24.848 495 262 17 9 473 2 416 8.52e-15 77.0
MS.gene09367.t1 AT2G04230 26.033 484 273 17 8 484 12 417 8.61e-15 77.0
MS.gene09367.t1 AT1G78750 25.587 426 226 16 2 403 11 369 1.84e-14 75.9
MS.gene09367.t1 AT1G78750 25.587 426 226 16 2 403 11 369 1.84e-14 75.9
MS.gene09367.t1 AT3G42770 28.986 345 180 13 14 350 4 291 1.87e-14 76.3
MS.gene09367.t1 AT3G26920 24.793 363 232 12 147 496 4 338 1.89e-14 75.1
MS.gene09367.t1 AT3G26920 24.793 363 232 12 147 496 4 338 1.89e-14 75.1
MS.gene09367.t1 AT5G56440 25.000 488 260 20 9 477 2 402 3.06e-14 75.1
MS.gene09367.t1 AT3G03030 26.829 369 191 10 9 352 2 316 3.93e-14 73.9
MS.gene09367.t1 AT1G69630 25.653 421 223 14 2 402 11 361 4.79e-14 74.7
MS.gene09367.t1 AT5G22670 26.148 501 255 22 8 484 10 419 7.07e-14 73.9
MS.gene09367.t1 AT2G29930 27.446 419 196 20 9 402 12 347 8.63e-14 73.9
MS.gene09367.t1 AT2G29930 27.446 419 196 20 9 402 12 347 8.63e-14 73.9
MS.gene09367.t1 AT5G56370 27.979 386 227 16 124 496 57 404 2.14e-13 72.4
MS.gene09367.t1 AT5G56370 27.979 386 227 16 124 496 57 404 2.14e-13 72.4
MS.gene09367.t1 AT2G42730 25.309 486 269 18 9 472 5 418 3.59e-13 72.0
MS.gene09367.t1 AT3G59240 24.877 406 230 12 9 401 8 351 4.33e-13 72.0
MS.gene09367.t1 AT1G66300 24.612 516 274 21 4 494 24 449 8.02e-13 70.9
MS.gene09367.t1 AT5G56820 22.822 482 262 17 9 480 15 396 1.10e-12 70.5
MS.gene09367.t1 AT5G56690 27.754 472 255 20 11 472 4 399 1.82e-12 69.3
MS.gene09367.t1 AT1G05080 27.219 338 197 13 152 472 103 408 1.86e-12 69.7
MS.gene09367.t1 AT4G22280 30.293 307 178 14 122 412 54 340 5.37e-12 68.2
MS.gene09367.t1 AT3G54160 27.964 329 195 12 96 402 22 330 5.50e-12 68.2
MS.gene09367.t1 AT4G22280 31.104 299 170 14 122 404 54 332 6.33e-12 67.4
MS.gene09367.t1 AT5G35995 26.333 300 171 12 137 409 78 354 1.10e-11 67.4
MS.gene09367.t1 AT5G35995 26.333 300 171 12 137 409 78 354 1.10e-11 67.4
MS.gene09367.t1 AT2G29930 32.922 243 111 14 182 402 15 227 1.54e-11 66.2
MS.gene09367.t1 AT5G25860 25.118 422 233 15 9 409 12 371 1.97e-11 66.6
MS.gene09367.t1 AT5G56390 26.129 310 186 10 176 475 119 395 2.89e-11 65.9
MS.gene09367.t1 AT1G78760 27.949 390 208 18 124 480 70 419 4.41e-11 65.5
MS.gene09367.t1 AT1G13780 24.040 495 259 21 9 470 10 420 6.93e-11 64.7

Find 97 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATTACATTTGAATTTAA+AGG 0.124626 4.1:+42099732 MS.gene09367:CDS
TAAAGAATTGGTATCTTCTT+TGG 0.229891 4.1:-42098254 None:intergenic
AGAAATCCTCCTTACTAGTA+TGG 0.246783 4.1:+42099889 MS.gene09367:CDS
CTAAGGCTTGATAGCGAAAA+AGG 0.263082 4.1:+42098424 MS.gene09367:CDS
GAAACTTACATGAAAAGTTT+TGG 0.271530 4.1:-42099049 None:intergenic
CTATATTCTCGGAGCGTCTT+TGG 0.278515 4.1:-42098288 None:intergenic
ATTGCAAGATCCTTAAGTTT+TGG 0.285282 4.1:-42099284 None:intergenic
TAAAGATTGGAATTCTTCTT+TGG 0.289144 4.1:-42098197 None:intergenic
GCAGATCGACTCCCATCTTT+TGG 0.302315 4.1:-42098119 None:intergenic
GGACAGCAAGATTTAAAGAT+TGG 0.302591 4.1:-42098210 None:intergenic
TCTATCTGGTTGTCCACATT+TGG 0.307537 4.1:+42098723 MS.gene09367:CDS
CTATATTCTCGGAGCGTCTT+AGG 0.313964 4.1:-42098231 None:intergenic
ATCTTCTTTGGCTATATTCT+CGG 0.349747 4.1:-42098242 None:intergenic
AACTCAGGGTCTGAAATTTC+TGG 0.368291 4.1:-42098172 None:intergenic
GAAAGCAAGCTGTAAAGAAT+TGG 0.371522 4.1:-42098266 None:intergenic
TATTAGTTTGACTATATTCT+CGG 0.399370 4.1:-42098299 None:intergenic
GAAAATTTGAAATCAATTCC+CGG 0.407633 4.1:+42099643 MS.gene09367:CDS
ACTCGTGGTCTTAAAATTGC+GGG 0.408358 4.1:+42098573 MS.gene09367:CDS
AAGAGCAGCTTCATCGAGAT+TGG 0.416322 4.1:-42098944 None:intergenic
ACAGATCCAAGTCAAGTTCC+TGG 0.422138 4.1:-42098501 None:intergenic
ACAGAATGCTCCAAAACTTA+AGG 0.424972 4.1:+42099274 MS.gene09367:CDS
GCCACATGATGTACCCTTGT+GGG 0.431302 4.1:+42098345 MS.gene09367:CDS
ATCTGGTTGTCCACATTTGG+AGG 0.433975 4.1:+42098726 MS.gene09367:CDS
AGGAGGTTGGAAACTCTAGA+TGG 0.436425 4.1:+42099670 MS.gene09367:CDS
GGTAAATGAAAGTGAAGGTT+GGG 0.445905 4.1:-42098151 None:intergenic
AATTTGTATACACTCTTGAA+AGG 0.452090 4.1:+42098994 MS.gene09367:CDS
CAAGGTCCAGGAACTTGACT+TGG 0.457085 4.1:+42098495 MS.gene09367:CDS
AGTTTCCAACCTCCTCTCTC+CGG 0.464355 4.1:-42099662 None:intergenic
ACTAATACTTGACAGTAAGA+TGG 0.466673 4.1:+42098315 MS.gene09367:CDS
AAATTCAAATGTAATGAGAC+TGG 0.466724 4.1:-42099727 None:intergenic
CATGTGTTCTATCCAAAAGA+TGG 0.467948 4.1:+42098107 MS.gene09367:CDS
AAAGTGAAGGTTGGGAATCT+TGG 0.470105 4.1:-42098143 None:intergenic
GTGACTCACACGTGGAAAGC+TGG 0.470578 4.1:-42098552 None:intergenic
TCAAAACATTAAAACTGAGT+TGG 0.477665 4.1:+42099758 MS.gene09367:CDS
GTATACAAATTGTTAAAATC+CGG 0.478212 4.1:-42098982 None:intergenic
TCTTTGCATTTGCAAGCGTC+CGG 0.481989 4.1:+42098963 MS.gene09367:CDS
AAGGATCTTGCAATTGCGGT+TGG 0.485603 4.1:+42099293 MS.gene09367:CDS
ATTTGTATACACTCTTGAAA+GGG 0.488919 4.1:+42098995 MS.gene09367:CDS
AAGTAGGCATATATAACAAA+AGG 0.493857 4.1:-42098816 None:intergenic
ACTTAAGGATCTTGCAATTG+CGG 0.496044 4.1:+42099289 MS.gene09367:CDS
GGCATTAGATCTCAAGGTCC+AGG 0.496808 4.1:+42098483 MS.gene09367:CDS
AATTCTTCTTTGGACAACTC+AGG 0.497729 4.1:-42098187 None:intergenic
AATTCGTACCCATACTAGTA+AGG 0.501044 4.1:-42099898 None:intergenic
GCTTTCTAATGTTAAAACAT+TGG 0.503057 4.1:+42099017 MS.gene09367:CDS
TGTACCCTTGTGGGTTTCAA+AGG 0.504188 4.1:+42098354 MS.gene09367:CDS
CTAATACTTGACAGTAAGAT+GGG 0.504263 4.1:+42098316 MS.gene09367:CDS
TGGTAAATGAAAGTGAAGGT+TGG 0.505664 4.1:-42098152 None:intergenic
TAGAATACGCCACAGTTTCA+CGG 0.516230 4.1:-42098663 None:intergenic
AATGTTAAAACATTGGCTCT+CGG 0.516570 4.1:+42099024 MS.gene09367:CDS
TATGAAGAACTTCAAGTGAC+GGG 0.519558 4.1:-42098873 None:intergenic
GAAATCCTCCTTACTAGTAT+GGG 0.519980 4.1:+42099890 MS.gene09367:CDS
GTTTCCAACCTCCTCTCTCC+GGG 0.520079 4.1:-42099661 None:intergenic
TCTCTTCAATAAGTGAAAAG+AGG 0.524063 4.1:-42098788 None:intergenic
ATTCTTCTTTGGACAACTCA+GGG 0.524121 4.1:-42098186 None:intergenic
GCGTATTCTATGTTTGATGA+TGG 0.525386 4.1:+42098676 MS.gene09367:CDS
TGTACACATGTGGGTTTCAA+AGG 0.528762 4.1:+42098462 MS.gene09367:CDS
CCGAAAATATAAGAAAACTA+AGG 0.538047 4.1:+42098407 MS.gene09367:CDS
CACTCGTGGTCTTAAAATTG+CGG 0.539007 4.1:+42098572 MS.gene09367:CDS
TTCAAAGGCATTAGATCTCA+AGG 0.542872 4.1:+42098477 MS.gene09367:CDS
TGTTATGCAGTTTCTCAGCA+TGG 0.553413 4.1:+42099148 MS.gene09367:intron
ATGTGTTCTATCCAAAAGAT+GGG 0.563690 4.1:+42098108 MS.gene09367:CDS
GACGGGGCATTAATTTGTAG+TGG 0.566180 4.1:-42098856 None:intergenic
TGAAATCAATTCCCGGAGAG+AGG 0.569941 4.1:+42099650 MS.gene09367:CDS
AAATATCTGAGAATTGCAGT+TGG 0.586019 4.1:+42099231 MS.gene09367:CDS
CAACACTTAATAGAAGAAGT+AGG 0.587492 4.1:-42098832 None:intergenic
AGACTGGTTGTAAGACATGC+CGG 0.589947 4.1:-42099711 None:intergenic
ATATGAAGAACTTCAAGTGA+CGG 0.594331 4.1:-42098874 None:intergenic
GGTGGAGCCATCAGTAACTC+CGG 0.595318 4.1:+42099692 MS.gene09367:CDS
ACAAGGGTACATCATGTGGC+TGG 0.596859 4.1:-42098342 None:intergenic
TCACACGTGGAAAGCTGGAG+CGG 0.598731 4.1:-42098547 None:intergenic
AAAAGAAGTTCCTCCAAATG+TGG 0.603065 4.1:-42098736 None:intergenic
AAGTGAAAAGAGGTATTGAT+CGG 0.603172 4.1:-42098778 None:intergenic
AATTCCCGGAGAGAGGAGGT+TGG 0.606183 4.1:+42099657 MS.gene09367:CDS
GCAATTCTCAGATATTTCAA+CGG 0.608159 4.1:-42099225 None:intergenic
ACCCACAAGGGTACATCATG+TGG 0.608519 4.1:-42098346 None:intergenic
AGACATGCCGGAGTTACTGA+TGG 0.614504 4.1:-42099699 None:intergenic
GGAGGTTGGAAACTCTAGAT+GGG 0.616468 4.1:+42099671 MS.gene09367:CDS
GGTATTGATCGGTAATGCAA+AGG 0.618727 4.1:-42098767 None:intergenic
TGAAGAGAAGAATTGAGTGT+TGG 0.626009 4.1:-42098607 None:intergenic
TCACTCAAGTAAGTTACAGA+AGG 0.626299 4.1:+42099803 MS.gene09367:CDS
AGCCACATGATGTACCCTTG+TGG 0.630082 4.1:+42098344 MS.gene09367:CDS
TCTTCATCTCCGTGAAACTG+TGG 0.632446 4.1:+42098654 MS.gene09367:CDS
TTCCACGTGTGAGTCACTCG+TGG 0.634670 4.1:+42098558 MS.gene09367:CDS
GGTTGGAAACTCTAGATGGG+TGG 0.637888 4.1:+42099674 MS.gene09367:CDS
AATCAATTCCCGGAGAGAGG+AGG 0.640354 4.1:+42099653 MS.gene09367:CDS
TTTCTGGTAAATGAAAGTGA+AGG 0.651983 4.1:-42098156 None:intergenic
ACATGTGTACATCATGTGAC+TGG 0.652666 4.1:-42098450 None:intergenic
AGATGAAGAATCTTTAGTGA+CGG 0.657169 4.1:-42098640 None:intergenic
TCGTACCCATACTAGTAAGG+AGG 0.668739 4.1:-42099895 None:intergenic
AAATGCCTTTGAAACCCACA+AGG 0.669831 4.1:-42098359 None:intergenic
CTCGTGGTCTTAAAATTGCG+GGG 0.673910 4.1:+42098574 MS.gene09367:CDS
GTCACATGATGTACACATGT+GGG 0.678429 4.1:+42098453 MS.gene09367:CDS
GACCACGAGTGACTCACACG+TGG 0.682798 4.1:-42098560 None:intergenic
TATGCAGTTTCTCAGCATGG+AGG 0.703511 4.1:+42099151 MS.gene09367:intron
AGTCACATGATGTACACATG+TGG 0.706369 4.1:+42098452 MS.gene09367:CDS
ATGAAGAACTTCAAGTGACG+GGG 0.715969 4.1:-42098872 None:intergenic
AATGCCTTTGAAACCCACAA+GGG 0.747260 4.1:-42098358 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATACAAATGTTAATTTT+TGG + chr4.1:42099608-42099627 MS.gene09367:intron 10.0%
!! AGATAAAATTGTAAGTAAAA+TGG - chr4.1:42099540-42099559 None:intergenic 15.0%
!! AAGTAAAATGGTTTAAAAGA+AGG - chr4.1:42099528-42099547 None:intergenic 20.0%
!! CTCATTACATTTGAATTTAA+AGG + chr4.1:42099732-42099751 MS.gene09367:CDS 20.0%
!! GAATTAAGTAATTCGTTTAA+CGG - chr4.1:42099455-42099474 None:intergenic 20.0%
!! GTATACAAATTGTTAAAATC+CGG - chr4.1:42098985-42099004 None:intergenic 20.0%
!!! CCTTAGTTTTCTTATATTTT+CGG - chr4.1:42098410-42098429 None:intergenic 20.0%
!!! TATTAGTTTGACTATATTCT+CGG - chr4.1:42098302-42098321 None:intergenic 20.0%
! AAATTCAAATGTAATGAGAC+TGG - chr4.1:42099730-42099749 None:intergenic 25.0%
! AACATAAGAAGTTGAAAATC+TGG + chr4.1:42099378-42099397 MS.gene09367:intron 25.0%
! AAGAACTAGTAAAAGATACA+TGG + chr4.1:42099414-42099433 MS.gene09367:intron 25.0%
! AAGTAGGCATATATAACAAA+AGG - chr4.1:42098819-42098838 None:intergenic 25.0%
! AATGAGAATAGAATTGCATA+TGG - chr4.1:42099119-42099138 None:intergenic 25.0%
! AATTTGTATACACTCTTGAA+AGG + chr4.1:42098994-42099013 MS.gene09367:CDS 25.0%
! ACTATATGCTATATATCAGT+TGG + chr4.1:42099585-42099604 MS.gene09367:intron 25.0%
! ATGAGAATAGAATTGCATAT+GGG - chr4.1:42099118-42099137 None:intergenic 25.0%
! ATTTGTATACACTCTTGAAA+GGG + chr4.1:42098995-42099014 MS.gene09367:CDS 25.0%
! CCGAAAATATAAGAAAACTA+AGG + chr4.1:42098407-42098426 MS.gene09367:CDS 25.0%
! GCTTTCTAATGTTAAAACAT+TGG + chr4.1:42099017-42099036 MS.gene09367:CDS 25.0%
! TAAAGAATTGGTATCTTCTT+TGG - chr4.1:42098257-42098276 None:intergenic 25.0%
! TAAAGATTGGAATTCTTCTT+TGG - chr4.1:42098200-42098219 None:intergenic 25.0%
! TCAAAACATTAAAACTGAGT+TGG + chr4.1:42099758-42099777 MS.gene09367:CDS 25.0%
!! GAAAATTTGAAATCAATTCC+CGG + chr4.1:42099643-42099662 MS.gene09367:CDS 25.0%
!! GAAACTTACATGAAAAGTTT+TGG - chr4.1:42099052-42099071 None:intergenic 25.0%
!! GATCTCAATAATTTTCTATC+TGG + chr4.1:42098709-42098728 MS.gene09367:CDS 25.0%
!! TATAAGACTCTTTTGTTGAT+AGG - chr4.1:42098084-42098103 None:intergenic 25.0%
!!! TAATTTTTGGTTTACATGCA+GGG + chr4.1:42099621-42099640 MS.gene09367:intron 25.0%
!!! TTAATTTTTGGTTTACATGC+AGG + chr4.1:42099620-42099639 MS.gene09367:intron 25.0%
!!! TTTCTGAAGTCATATTTTCT+AGG - chr4.1:42098919-42098938 None:intergenic 25.0%
AAATATCTGAGAATTGCAGT+TGG + chr4.1:42099231-42099250 MS.gene09367:CDS 30.0%
ACTAATACTTGACAGTAAGA+TGG + chr4.1:42098315-42098334 MS.gene09367:CDS 30.0%
AGATGAAGAATCTTTAGTGA+CGG - chr4.1:42098643-42098662 None:intergenic 30.0%
AGTAGAAAAAAGTTTACGCA+AGG + chr4.1:42099860-42099879 MS.gene09367:CDS 30.0%
ATATGAAGAACTTCAAGTGA+CGG - chr4.1:42098877-42098896 None:intergenic 30.0%
ATCTTCTTTGGCTATATTCT+CGG - chr4.1:42098245-42098264 None:intergenic 30.0%
ATGTGTTCTATCCAAAAGAT+GGG + chr4.1:42098108-42098127 MS.gene09367:CDS 30.0%
CAACACTTAATAGAAGAAGT+AGG - chr4.1:42098835-42098854 None:intergenic 30.0%
CTAATACTTGACAGTAAGAT+GGG + chr4.1:42098316-42098335 MS.gene09367:CDS 30.0%
GCAATTCTCAGATATTTCAA+CGG - chr4.1:42099228-42099247 None:intergenic 30.0%
GTAGAAAAAAGTTTACGCAA+GGG + chr4.1:42099861-42099880 MS.gene09367:CDS 30.0%
TAGAAAAAAGTTTACGCAAG+GGG + chr4.1:42099862-42099881 MS.gene09367:CDS 30.0%
TCTCTTCAATAAGTGAAAAG+AGG - chr4.1:42098791-42098810 None:intergenic 30.0%
! ATTGCAAGATCCTTAAGTTT+TGG - chr4.1:42099287-42099306 None:intergenic 30.0%
! TTTCTGGTAAATGAAAGTGA+AGG - chr4.1:42098159-42098178 None:intergenic 30.0%
!! AAGTGAAAAGAGGTATTGAT+CGG - chr4.1:42098781-42098800 None:intergenic 30.0%
!! AATGTTAAAACATTGGCTCT+CGG + chr4.1:42099024-42099043 MS.gene09367:CDS 30.0%
AAAAGAAGTTCCTCCAAATG+TGG - chr4.1:42098739-42098758 None:intergenic 35.0%
AATTCTTCTTTGGACAACTC+AGG - chr4.1:42098190-42098209 None:intergenic 35.0%
ACAGAATGCTCCAAAACTTA+AGG + chr4.1:42099274-42099293 MS.gene09367:CDS 35.0%
ACTTAAGGATCTTGCAATTG+CGG + chr4.1:42099289-42099308 MS.gene09367:CDS 35.0%
AGAAATCCTCCTTACTAGTA+TGG + chr4.1:42099889-42099908 MS.gene09367:CDS 35.0%
ATTCTTCTTTGGACAACTCA+GGG - chr4.1:42098189-42098208 None:intergenic 35.0%
CATGTGTTCTATCCAAAAGA+TGG + chr4.1:42098107-42098126 MS.gene09367:CDS 35.0%
GAAAGCAAGCTGTAAAGAAT+TGG - chr4.1:42098269-42098288 None:intergenic 35.0%
GCGTATTCTATGTTTGATGA+TGG + chr4.1:42098676-42098695 MS.gene09367:CDS 35.0%
GGACAGCAAGATTTAAAGAT+TGG - chr4.1:42098213-42098232 None:intergenic 35.0%
GGTAAATGAAAGTGAAGGTT+GGG - chr4.1:42098154-42098173 None:intergenic 35.0%
TATGAAGAACTTCAAGTGAC+GGG - chr4.1:42098876-42098895 None:intergenic 35.0%
TCACTCAAGTAAGTTACAGA+AGG + chr4.1:42099803-42099822 MS.gene09367:CDS 35.0%
TGAAGAGAAGAATTGAGTGT+TGG - chr4.1:42098610-42098629 None:intergenic 35.0%
TGCATATGGGAGACTAAATA+TGG - chr4.1:42099105-42099124 None:intergenic 35.0%
TGGTAAATGAAAGTGAAGGT+TGG - chr4.1:42098155-42098174 None:intergenic 35.0%
!! TTATATTTTCGGTGCGTCTT+AGG - chr4.1:42098399-42098418 None:intergenic 35.0%
!! TTCAAAGGCATTAGATCTCA+AGG + chr4.1:42098477-42098496 MS.gene09367:CDS 35.0%
!!! GCGGCAATAAAACTTTTTCA+TGG - chr4.1:42098531-42098550 None:intergenic 35.0%
AAAGTGAAGGTTGGGAATCT+TGG - chr4.1:42098146-42098165 None:intergenic 40.0%
ACATGTGTACATCATGTGAC+TGG - chr4.1:42098453-42098472 None:intergenic 40.0%
AGTCACATGATGTACACATG+TGG + chr4.1:42098452-42098471 MS.gene09367:CDS 40.0%
ATGAAGAACTTCAAGTGACG+GGG - chr4.1:42098875-42098894 None:intergenic 40.0%
GTCACATGATGTACACATGT+GGG + chr4.1:42098453-42098472 MS.gene09367:CDS 40.0%
TAGAATACGCCACAGTTTCA+CGG - chr4.1:42098666-42098685 None:intergenic 40.0%
TCTATCTGGTTGTCCACATT+TGG + chr4.1:42098723-42098742 MS.gene09367:CDS 40.0%
TGTACACATGTGGGTTTCAA+AGG + chr4.1:42098462-42098481 MS.gene09367:CDS 40.0%
TGTTATGCAGTTTCTCAGCA+TGG + chr4.1:42099148-42099167 MS.gene09367:intron 40.0%
! AACTCAGGGTCTGAAATTTC+TGG - chr4.1:42098175-42098194 None:intergenic 40.0%
! ACTCGTGGTCTTAAAATTGC+GGG + chr4.1:42098573-42098592 MS.gene09367:CDS 40.0%
! CACTCGTGGTCTTAAAATTG+CGG + chr4.1:42098572-42098591 MS.gene09367:CDS 40.0%
!! AAATGCCTTTGAAACCCACA+AGG - chr4.1:42098362-42098381 None:intergenic 40.0%
!! AATGCCTTTGAAACCCACAA+GGG - chr4.1:42098361-42098380 None:intergenic 40.0%
!! CTAAGGCTTGATAGCGAAAA+AGG + chr4.1:42098424-42098443 MS.gene09367:CDS 40.0%
!! GGTATTGATCGGTAATGCAA+AGG - chr4.1:42098770-42098789 None:intergenic 40.0%
AAGAGCAGCTTCATCGAGAT+TGG - chr4.1:42098947-42098966 None:intergenic 45.0%
AAGGATCTTGCAATTGCGGT+TGG + chr4.1:42099293-42099312 MS.gene09367:CDS 45.0%
ACAGATCCAAGTCAAGTTCC+TGG - chr4.1:42098504-42098523 None:intergenic 45.0%
AGGAGGTTGGAAACTCTAGA+TGG + chr4.1:42099670-42099689 MS.gene09367:CDS 45.0%
ATCTGGTTGTCCACATTTGG+AGG + chr4.1:42098726-42098745 MS.gene09367:CDS 45.0%
GGAGGTTGGAAACTCTAGAT+GGG + chr4.1:42099671-42099690 MS.gene09367:CDS 45.0%
TATGCAGTTTCTCAGCATGG+AGG + chr4.1:42099151-42099170 MS.gene09367:intron 45.0%
TCTTCATCTCCGTGAAACTG+TGG + chr4.1:42098654-42098673 MS.gene09367:CDS 45.0%
TCTTTGCATTTGCAAGCGTC+CGG + chr4.1:42098963-42098982 MS.gene09367:CDS 45.0%
TGAAATCAATTCCCGGAGAG+AGG + chr4.1:42099650-42099669 MS.gene09367:CDS 45.0%
TGTACCCTTGTGGGTTTCAA+AGG + chr4.1:42098354-42098373 MS.gene09367:CDS 45.0%
! AGACTGGTTGTAAGACATGC+CGG - chr4.1:42099714-42099733 None:intergenic 45.0%
! CTATATTCTCGGAGCGTCTT+AGG - chr4.1:42098234-42098253 None:intergenic 45.0%
! CTATATTCTCGGAGCGTCTT+TGG - chr4.1:42098234-42098253 None:intergenic 45.0%
! CTCGTGGTCTTAAAATTGCG+GGG + chr4.1:42098574-42098593 MS.gene09367:CDS 45.0%
!! GACGGGGCATTAATTTGTAG+TGG - chr4.1:42098859-42098878 None:intergenic 45.0%
AATCAATTCCCGGAGAGAGG+AGG + chr4.1:42099653-42099672 MS.gene09367:CDS 50.0%
AGACATGCCGGAGTTACTGA+TGG - chr4.1:42099702-42099721 None:intergenic 50.0%
AGCCACATGATGTACCCTTG+TGG + chr4.1:42098344-42098363 MS.gene09367:CDS 50.0%
AGTTTCCAACCTCCTCTCTC+CGG - chr4.1:42099665-42099684 None:intergenic 50.0%
CAAGGTCCAGGAACTTGACT+TGG + chr4.1:42098495-42098514 MS.gene09367:CDS 50.0%
GCCACATGATGTACCCTTGT+GGG + chr4.1:42098345-42098364 MS.gene09367:CDS 50.0%
GGCATTAGATCTCAAGGTCC+AGG + chr4.1:42098483-42098502 MS.gene09367:CDS 50.0%
GGTTGGAAACTCTAGATGGG+TGG + chr4.1:42099674-42099693 MS.gene09367:CDS 50.0%
! ACAAGGGTACATCATGTGGC+TGG - chr4.1:42098345-42098364 None:intergenic 50.0%
! ACCCACAAGGGTACATCATG+TGG - chr4.1:42098349-42098368 None:intergenic 50.0%
!! GCAGATCGACTCCCATCTTT+TGG - chr4.1:42098122-42098141 None:intergenic 50.0%
AATTCCCGGAGAGAGGAGGT+TGG + chr4.1:42099657-42099676 MS.gene09367:CDS 55.0%
GGTGGAGCCATCAGTAACTC+CGG + chr4.1:42099692-42099711 MS.gene09367:CDS 55.0%
GTGACTCACACGTGGAAAGC+TGG - chr4.1:42098555-42098574 None:intergenic 55.0%
GTTTCCAACCTCCTCTCTCC+GGG - chr4.1:42099664-42099683 None:intergenic 55.0%
TCACACGTGGAAAGCTGGAG+CGG - chr4.1:42098550-42098569 None:intergenic 55.0%
TTCCACGTGTGAGTCACTCG+TGG + chr4.1:42098558-42098577 MS.gene09367:CDS 55.0%
GACCACGAGTGACTCACACG+TGG - chr4.1:42098563-42098582 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 42098007 42099911 42098007 ID=MS.gene09367
chr4.1 mRNA 42098007 42099911 42098007 ID=MS.gene09367.t1;Parent=MS.gene09367
chr4.1 exon 42098007 42099062 42098007 ID=MS.gene09367.t1.exon1;Parent=MS.gene09367.t1
chr4.1 CDS 42098007 42099062 42098007 ID=cds.MS.gene09367.t1;Parent=MS.gene09367.t1
chr4.1 exon 42099158 42099325 42099158 ID=MS.gene09367.t1.exon2;Parent=MS.gene09367.t1
chr4.1 CDS 42099158 42099325 42099158 ID=cds.MS.gene09367.t1;Parent=MS.gene09367.t1
chr4.1 exon 42099642 42099911 42099642 ID=MS.gene09367.t1.exon3;Parent=MS.gene09367.t1
chr4.1 CDS 42099642 42099911 42099642 ID=cds.MS.gene09367.t1;Parent=MS.gene09367.t1
Gene Sequence

>MS.gene09367

ATGGCGTGCTCTGATGAAGAACATGATCGATTAAGCAATCTCTCAGACGATTTGATTTGTCATATTTGTTCTTTCCTATCAACAAAAGAGTCTTATAGAACATGTGTTCTATCCAAAAGATGGGAGTCGATCTGCACCAAGATTCCCAACCTTCACTTTCATTTACCAGAAATTTCAGACCCTGAGTTGTCCAAAGAAGAATTCCAATCTTTAAATCTTGCTGTCCTAAGACGCTCCGAGAATATAGCCAAAGAAGATACCAATTCTTTACAGCTTGCTTTCCAAAGACGCTCCGAGAATATAGTCAAACTAATACTTGACAGTAAGATGGGCTGCCAGCCACATGATGTACCCTTGTGGGTTTCAAAGGCATTTGTAAATTTTGCTCTCCTAAGACGCACCGAAAATATAAGAAAACTAAGGCTTGATAGCGAAAAAGGCTGCCAGTCACATGATGTACACATGTGGGTTTCAAAGGCATTAGATCTCAAGGTCCAGGAACTTGACTTGGATCTGTTTCTCCATGAAAAAGTTTTATTGCCGCTCCAGCTTTCCACGTGTGAGTCACTCGTGGTCTTAAAATTGCGGGGCAGAATTCAACCAACACTCAATTCTTCTCTTCATGTATATTTACCGTCACTAAAGATTCTTCATCTCCGTGAAACTGTGGCGTATTCTATGTTTGATGATGGTAAAGAGTATGATCTCAATAATTTTCTATCTGGTTGTCCACATTTGGAGGAACTTCTTTTACATGAGACCTTTGCATTACCGATCAATACCTCTTTTCACTTATTGAAGAGATTATTCCTTTTGTTATATATGCCTACTTCTTCTATTAAGTGTTGTCCACTACAAATTAATGCCCCGTCACTTGAAGTTCTTCATATAGCTGATATTTCATTGACTCCTAGAAAATATGACTTCAGAAACTTGTCCAATCTCGATGAAGCTGCTCTTTGCATTTGCAAGCGTCCGGATTTTAACAATTTGTATACACTCTTGAAAGGGCTTTCTAATGTTAAAACATTGGCTCTCGGTTCCAAAACTTTTCATGTAAGTTTCATAAATATTTTTCTTTTTTAATTACAAGTTCCATATTTAGTCTCCCATATGCAATTCTATTCTCATTTTATGATTTTGTTATGCAGTTTCTCAGCATGGAGGACAAATTTGACAGCTTGCATCTTCTCACTTTTCACAATTTATTATTTTTAACCGTTGAAATATCTGAGAATTGCAGTTGGAATATGCTCATCAGCTTTCTACAGAATGCTCCAAAACTTAAGGATCTTGCAATTGCGGTTGGAATTCAGTGTGTATGAAGTTTTTTTAACTATATTTGCACAATATATTTTTTTTAAAAGATAAAACATAAGAAGTTGAAAATCTGGCACAAGAAGAGCAAAGAACTAGTAAAAGATACATGGAGTTTGCAAATAAAACCGTTAAACGAATTACTTAATTCAAAACAATCTATATCTGATCTATATTTGTGTAGATTTTCTAGCAATTTAACCTTCTTTTAAACCATTTTACTTACAATTTTATCTTTATTTTCTTAATAATGTAATTGAAACTATATGCTATATATCAGTTGGAAAATACAAATGTTAATTTTTGGTTTACATGCAGGGAAAATTTGAAATCAATTCCCGGAGAGAGGAGGTTGGAAACTCTAGATGGGTGGAGCCATCAGTAACTCCGGCATGTCTTACAACCAGTCTCATTACATTTGAATTTAAAGGAATTCAAAACATTAAAACTGAGTTGGACTTTACGCGATATATTGTAAGTCACTCAAGTAAGTTACAGAAGGTAAAGATTTTCACACTCAGTTCAAAGAAAAGAAGAGTAGAAAAAAGTTTACGCAAGGGGTCAAAGAAATCCTCCTTACTAGTATGG
Protein sequence

>MS.gene09367.t1

MACSDEEHDRLSNLSDDLICHICSFLSTKESYRTCVLSKRWESICTKIPNLHFHLPEISDPELSKEEFQSLNLAVLRRSENIAKEDTNSLQLAFQRRSENIVKLILDSKMGCQPHDVPLWVSKAFVNFALLRRTENIRKLRLDSEKGCQSHDVHMWVSKALDLKVQELDLDLFLHEKVLLPLQLSTCESLVVLKLRGRIQPTLNSSLHVYLPSLKILHLRETVAYSMFDDGKEYDLNNFLSGCPHLEELLLHETFALPINTSFHLLKRLFLLLYMPTSSIKCCPLQINAPSLEVLHIADISLTPRKYDFRNLSNLDEAALCICKRPDFNNLYTLLKGLSNVKTLALGSKTFHFLSMEDKFDSLHLLTFHNLLFLTVEISENCSWNMLISFLQNAPKLKDLAIAVGIQCGKFEINSRREEVGNSRWVEPSVTPACLTTSLITFEFKGIQNIKTELDFTRYIVSHSSKLQKVKIFTLSSKKRRVEKSLRKGSKKSSLLVW