AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07674


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07674.t1 MTR_4g056020 89.565 115 12 0 1 115 1 115 1.34e-67 214
MS.gene07674.t1 MTR_4g056020 90.411 73 7 0 107 179 224 296 1.81e-39 140
MS.gene07674.t1 MTR_4g056030 86.087 115 16 0 1 115 1 115 3.33e-64 205
MS.gene07674.t1 MTR_4g056030 76.712 73 17 0 107 179 224 296 2.05e-31 119
MS.gene07674.t1 MTR_4g055990 65.789 152 15 2 1 115 1 152 1.50e-55 183
MS.gene07674.t1 MTR_4g055990 86.301 73 10 0 107 179 261 333 1.55e-37 135
MS.gene07674.t1 MTR_4g055990 65.789 152 15 2 1 115 1 152 1.61e-55 183
MS.gene07674.t1 MTR_4g055990 86.301 73 10 0 107 179 261 333 1.26e-37 136
MS.gene07674.t1 MTR_4g056040 66.207 145 19 1 1 115 1 145 6.67e-55 182
MS.gene07674.t1 MTR_4g056040 75.000 72 18 0 108 179 255 326 1.78e-31 119
MS.gene07674.t1 MTR_4g055580 81.905 105 18 1 1 104 92 196 6.90e-54 181
MS.gene07674.t1 MTR_4g055580 84.932 73 11 0 107 179 351 423 3.12e-35 130
MS.gene07674.t1 MTR_4g056070 61.935 155 19 1 1 115 1 155 1.13e-53 177
MS.gene07674.t1 MTR_4g056070 78.082 73 16 0 107 179 264 336 4.79e-33 122
MS.gene07674.t1 MTR_1g090900 67.213 122 38 1 1 120 1 122 2.98e-51 164
MS.gene07674.t1 MTR_1g090883 61.364 132 51 0 2 133 12 143 1.55e-45 154
MS.gene07674.t1 MTR_1g090883 59.459 74 30 0 106 179 233 306 1.00e-21 91.3
MS.gene07674.t1 MTR_1g038710 68.908 119 33 1 1 115 11 129 3.92e-44 152
MS.gene07674.t1 MTR_1g038710 72.973 74 20 0 106 179 239 312 1.19e-29 114
MS.gene07674.t1 MTR_4g093690 59.322 118 46 1 1 118 10 125 4.55e-40 137
MS.gene07674.t1 MTR_7g110920 71.622 74 21 0 106 179 92 165 7.41e-30 112
MS.gene07674.t1 MTR_4g056010 74.468 47 12 0 69 115 2 48 4.73e-18 75.9
MS.gene07674.t1 MTR_7g033945 39.604 101 55 3 9 105 11 109 9.32e-13 63.9
MS.gene07674.t1 MTR_1g115245 31.548 168 94 6 6 161 23 181 2.18e-12 64.7
MS.gene07674.t1 MTR_7g033915 39.604 101 55 3 9 105 11 109 8.11e-12 63.5
MS.gene07674.t1 MTR_7g110910 78.947 38 8 0 1 38 158 195 1.35e-11 61.2
MS.gene07674.t1 MTR_7g007080 31.395 172 91 6 7 161 2 163 2.00e-11 62.4
MS.gene07674.t1 MTR_7g034110 33.871 124 75 3 9 132 11 127 3.19e-11 61.6
MS.gene07674.t1 MTR_4g092430 40.860 93 51 3 5 95 39 129 9.78e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07674.t1 AT4G13960 36.806 144 76 4 9 142 2 140 4.73e-15 73.2
MS.gene07674.t1 AT4G13960 36.806 144 76 4 9 142 2 140 4.73e-15 73.2
MS.gene07674.t1 AT3G03030 34.815 135 72 3 9 133 2 130 8.00e-13 66.2
MS.gene07674.t1 AT3G59000 31.148 183 99 7 9 180 2 168 1.07e-12 65.9
MS.gene07674.t1 AT3G03030 34.815 135 72 3 9 133 2 130 1.10e-12 66.2
MS.gene07674.t1 AT3G03040 39.252 107 55 2 9 105 2 108 2.66e-12 65.1
MS.gene07674.t1 AT1G55660 29.730 148 83 4 9 151 53 184 4.81e-12 64.3
MS.gene07674.t1 AT1G55660 30.405 148 82 4 9 151 53 184 5.01e-12 64.3
MS.gene07674.t1 AT4G00315 35.294 102 61 2 9 105 2 103 7.07e-12 63.9
MS.gene07674.t1 AT5G38392 30.108 186 113 6 9 182 2 182 1.89e-11 61.2
MS.gene07674.t1 AT1G19070 60.000 45 18 0 9 53 2 46 2.14e-11 58.2
MS.gene07674.t1 AT3G59000 38.532 109 55 4 9 106 2 109 2.31e-11 62.4
MS.gene07674.t1 AT5G56440 33.645 107 70 1 9 114 2 108 2.72e-11 62.0
MS.gene07674.t1 AT3G59190 38.889 108 56 4 9 106 12 119 3.17e-11 62.0
MS.gene07674.t1 AT3G59210 38.095 105 57 3 9 105 7 111 4.01e-11 61.6
MS.gene07674.t1 AT3G59210 38.095 105 57 3 9 105 7 111 4.01e-11 61.6
MS.gene07674.t1 AT3G59210 38.095 105 57 3 9 105 7 111 4.01e-11 61.6
MS.gene07674.t1 AT3G59210 38.095 105 57 3 9 105 7 111 4.01e-11 61.6
MS.gene07674.t1 AT3G59210 38.095 105 57 3 9 105 7 111 4.01e-11 61.6
MS.gene07674.t1 AT3G59190 38.889 108 56 4 9 106 12 119 4.11e-11 61.6

Find 31 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAAATTTGATTTCTT+TGG 0.190993 4.1:-42521242 None:intergenic
ATATAATAGGCTCAAAAGTC+TGG 0.400206 4.1:+42522244 MS.gene07674:intron
AAAGTGAAGGTTGGGAATCT+TGG 0.417455 4.1:-42521188 None:intergenic
GGTAATTGAAAGTGAAGGTT+GGG 0.443422 4.1:-42521196 None:intergenic
GTATACAAATTGTTAAAATC+CGG 0.478212 4.1:-42521858 None:intergenic
TTGATTTCTTTGGACAACTC+AGG 0.483223 4.1:-42521232 None:intergenic
TCTTTGCATTTGTAAGCGTC+CGG 0.498367 4.1:+42521839 MS.gene07674:CDS
AATTTGTATACACTCTTGAA+AGG 0.500652 4.1:+42521870 MS.gene07674:CDS
TGATTTCTTTGGACAACTCA+GGG 0.514750 4.1:-42521231 None:intergenic
AACTCAGGGTCTGAAATCTC+TGG 0.516045 4.1:-42521217 None:intergenic
TGTACACATGTGGGTTTCAA+AGG 0.528762 4.1:+42521339 MS.gene07674:CDS
TAGAAAGAACTTCAAGTGAA+GGG 0.540099 4.1:-42521749 None:intergenic
CATGTGTTCTATCCACAAGA+TGG 0.542093 4.1:+42521152 MS.gene07674:CDS
TTCAAAGGCATTAGATCTCA+AGG 0.542872 4.1:+42521354 MS.gene07674:CDS
TGGTAATTGAAAGTGAAGGT+TGG 0.551378 4.1:-42521197 None:intergenic
CTAAGTCTTGATAGCAACAA+AGG 0.551526 4.1:+42521301 MS.gene07674:CDS
ATAGAAAGAACTTCAAGTGA+AGG 0.561374 4.1:-42521750 None:intergenic
ACTTTCTAATGTCAAAACAT+TGG 0.567332 4.1:+42521893 MS.gene07674:CDS
GAAGGGGCATTAATTTGTAG+TGG 0.570310 4.1:-42521732 None:intergenic
GCATATCGACTCCCATCTTG+TGG 0.572297 4.1:-42521164 None:intergenic
GCAGTAGAAGAGCAAAGACT+TGG 0.579384 4.1:+42522266 MS.gene07674:CDS
ATGTGTTCTATCCACAAGAT+GGG 0.579779 4.1:+42521153 MS.gene07674:CDS
AAGACTTGGCAAAATATACA+TGG 0.585450 4.1:+42522280 MS.gene07674:CDS
AATGTCAAAACATTGGCTCT+CGG 0.596249 4.1:+42521900 MS.gene07674:CDS
AGAAAGAACTTCAAGTGAAG+GGG 0.603765 4.1:-42521748 None:intergenic
AAGAGCAGCTTCATCAACAG+TGG 0.644416 4.1:-42521820 None:intergenic
GCCACATGATGTACACATGT+GGG 0.650684 4.1:+42521330 MS.gene07674:CDS
ACATGTGTACATCATGTGGC+TGG 0.656362 4.1:-42521327 None:intergenic
ACCCACATGTGTACATCATG+TGG 0.672342 4.1:-42521331 None:intergenic
AGCCACATGATGTACACATG+TGG 0.685172 4.1:+42521329 MS.gene07674:CDS
TCTCTGGTAATTGAAAGTGA+AGG 0.696256 4.1:-42521201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAGATGAAATATAAT+AGG + chr4.1:42522231-42522250 MS.gene07674:intron 10.0%
!!! ACTTAGTTTTCTTATATTTT+CGG - chr4.1:42521287-42521306 None:intergenic 15.0%
!! ATAATAAATTGTGAAAAGTG+AGG - chr4.1:42522075-42522094 None:intergenic 20.0%
!! GTATACAAATTGTTAAAATC+CGG - chr4.1:42521861-42521880 None:intergenic 20.0%
!!! ATTCACAAATTTGATTTCTT+TGG - chr4.1:42521245-42521264 None:intergenic 20.0%
!!! TGATCTAATAATTTTCTATC+TGG + chr4.1:42521585-42521604 MS.gene07674:intron 20.0%
!!! TTCTATTGTTGATTTTTCAT+TGG + chr4.1:42521767-42521786 MS.gene07674:CDS 20.0%
!!! TTTGTAAAGTCATATTTTCT+AGG - chr4.1:42521795-42521814 None:intergenic 20.0%
! AAGTAGGCATATATAAAGTA+AGG - chr4.1:42521695-42521714 None:intergenic 25.0%
! AATGAGAATAGAATTGCATA+TGG - chr4.1:42521995-42522014 None:intergenic 25.0%
! AATTTGTATACACTCTTGAA+AGG + chr4.1:42521870-42521889 MS.gene07674:CDS 25.0%
! ACTTTCTAATGTCAAAACAT+TGG + chr4.1:42521893-42521912 MS.gene07674:CDS 25.0%
! ATGAGAATAGAATTGCATAT+GGG - chr4.1:42521994-42522013 None:intergenic 25.0%
! TACTTTATATATGCCTACTT+CGG + chr4.1:42521695-42521714 MS.gene07674:intron 25.0%
!! AAAAAATCTTATTGCCACTT+AGG + chr4.1:42521410-42521429 MS.gene07674:intron 25.0%
!! TATAAGACTCTTTTGTTGAT+AGG - chr4.1:42521129-42521148 None:intergenic 25.0%
AAATATCTGAGAATTGCAGT+TGG + chr4.1:42522107-42522126 MS.gene07674:intron 30.0%
AAGACTTGGCAAAATATACA+TGG + chr4.1:42522280-42522299 MS.gene07674:CDS 30.0%
ACTTAAGGATCTTGCAATTA+TGG + chr4.1:42522165-42522184 MS.gene07674:intron 30.0%
AGATGAAGAATCTTTAGTGA+CGG - chr4.1:42521520-42521539 None:intergenic 30.0%
ATAGAAAGAACTTCAAGTGA+AGG - chr4.1:42521753-42521772 None:intergenic 30.0%
ATATAATAGGCTCAAAAGTC+TGG + chr4.1:42522244-42522263 MS.gene07674:intron 30.0%
TAGAAAGAACTTCAAGTGAA+GGG - chr4.1:42521752-42521771 None:intergenic 30.0%
TCTCTTCAATAAGTGAAAAG+AGG - chr4.1:42521667-42521686 None:intergenic 30.0%
TGAAAAGAAGAATTGAGTGT+TGG - chr4.1:42521487-42521506 None:intergenic 30.0%
! ATTGCAAGATCCTTAAGTTT+TGG - chr4.1:42522163-42522182 None:intergenic 30.0%
!!! GTGGCAATAAGATTTTTTCA+TGG - chr4.1:42521408-42521427 None:intergenic 30.0%
AAAAGAAGTTCCTCTAGATG+TGG - chr4.1:42521615-42521634 None:intergenic 35.0%
ACAGAATGCTCCAAAACTTA+AGG + chr4.1:42522150-42522169 MS.gene07674:intron 35.0%
AGAAAGAACTTCAAGTGAAG+GGG - chr4.1:42521751-42521770 None:intergenic 35.0%
ATGTGTTCTATCCACAAGAT+GGG + chr4.1:42521153-42521172 MS.gene07674:CDS 35.0%
CAACACTTAATAACCGAAGT+AGG - chr4.1:42521711-42521730 None:intergenic 35.0%
GGTAATTGAAAGTGAAGGTT+GGG - chr4.1:42521199-42521218 None:intergenic 35.0%
TCTCTGGTAATTGAAAGTGA+AGG - chr4.1:42521204-42521223 None:intergenic 35.0%
TGATTTCTTTGGACAACTCA+GGG - chr4.1:42521234-42521253 None:intergenic 35.0%
TGCATATGGGTGACTAAATT+TGG - chr4.1:42521981-42522000 None:intergenic 35.0%
TGGTAATTGAAAGTGAAGGT+TGG - chr4.1:42521200-42521219 None:intergenic 35.0%
TTGATTTCTTTGGACAACTC+AGG - chr4.1:42521235-42521254 None:intergenic 35.0%
! CTAAGTCTTGATAGCAACAA+AGG + chr4.1:42521301-42521320 MS.gene07674:CDS 35.0%
!! AAGTGAAAAGAGGTGTTGAT+AGG - chr4.1:42521657-42521676 None:intergenic 35.0%
!! AATGTCAAAACATTGGCTCT+CGG + chr4.1:42521900-42521919 MS.gene07674:CDS 35.0%
!! TTATATTTTCGGTGCGTCTT+AGG - chr4.1:42521276-42521295 None:intergenic 35.0%
!! TTCAAAGGCATTAGATCTCA+AGG + chr4.1:42521354-42521373 MS.gene07674:CDS 35.0%
AAAGTGAAGGTTGGGAATCT+TGG - chr4.1:42521191-42521210 None:intergenic 40.0%
ACAGATCCAAGTCAAGTTTC+TGG - chr4.1:42521381-42521400 None:intergenic 40.0%
CATGTGTTCTATCCACAAGA+TGG + chr4.1:42521152-42521171 MS.gene07674:CDS 40.0%
GATAGGTAATGCAAAGGTCT+CGG - chr4.1:42521640-42521659 None:intergenic 40.0%
TAGAATACGCCACAGTTTCA+CGG - chr4.1:42521543-42521562 None:intergenic 40.0%
TCTTTGCATTTGTAAGCGTC+CGG + chr4.1:42521839-42521858 MS.gene07674:CDS 40.0%
TGTACACATGTGGGTTTCAA+AGG + chr4.1:42521339-42521358 MS.gene07674:CDS 40.0%
TGTTATGCAGTTTCTCAGCA+TGG + chr4.1:42522024-42522043 MS.gene07674:intron 40.0%
! ACTCGTGGTGTTAAAATTGC+GGG + chr4.1:42521450-42521469 MS.gene07674:intron 40.0%
! CACTCGTGGTGTTAAAATTG+CGG + chr4.1:42521449-42521468 MS.gene07674:intron 40.0%
!! GAAGGGGCATTAATTTGTAG+TGG - chr4.1:42521735-42521754 None:intergenic 40.0%
!! GGTGTTGATAGGTAATGCAA+AGG - chr4.1:42521646-42521665 None:intergenic 40.0%
AAGAGCAGCTTCATCAACAG+TGG - chr4.1:42521823-42521842 None:intergenic 45.0%
ACATGTGTACATCATGTGGC+TGG - chr4.1:42521330-42521349 None:intergenic 45.0%
ACCCACATGTGTACATCATG+TGG - chr4.1:42521334-42521353 None:intergenic 45.0%
AGCCACATGATGTACACATG+TGG + chr4.1:42521329-42521348 MS.gene07674:CDS 45.0%
ATCTGGTTGTCCACATCTAG+AGG + chr4.1:42521602-42521621 MS.gene07674:intron 45.0%
CAAGGTCCAGAAACTTGACT+TGG + chr4.1:42521372-42521391 MS.gene07674:intron 45.0%
GCAGTAGAAGAGCAAAGACT+TGG + chr4.1:42522266-42522285 MS.gene07674:CDS 45.0%
GCCACATGATGTACACATGT+GGG + chr4.1:42521330-42521349 MS.gene07674:CDS 45.0%
TATGCAGTTTCTCAGCATGG+AGG + chr4.1:42522027-42522046 MS.gene07674:intron 45.0%
TCACACGTGAAAAGCCTAAG+TGG - chr4.1:42521427-42521446 None:intergenic 45.0%
TCTTCATCTCCGTGAAACTG+TGG + chr4.1:42521531-42521550 MS.gene07674:intron 45.0%
! AACTCAGGGTCTGAAATCTC+TGG - chr4.1:42521220-42521239 None:intergenic 45.0%
! CTCGTGGTGTTAAAATTGCG+GGG + chr4.1:42521451-42521470 MS.gene07674:intron 45.0%
TTTCACGTGTGAGTCACTCG+TGG + chr4.1:42521435-42521454 MS.gene07674:intron 50.0%
!! GCATATCGACTCCCATCTTG+TGG - chr4.1:42521167-42521186 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 42521052 42522315 42521052 ID=MS.gene07674
chr4.1 mRNA 42521052 42522315 42521052 ID=MS.gene07674.t1;Parent=MS.gene07674
chr4.1 exon 42521052 42521375 42521052 ID=MS.gene07674.t1.exon1;Parent=MS.gene07674.t1
chr4.1 CDS 42521052 42521375 42521052 ID=cds.MS.gene07674.t1;Parent=MS.gene07674.t1
chr4.1 exon 42521726 42521938 42521726 ID=MS.gene07674.t1.exon2;Parent=MS.gene07674.t1
chr4.1 CDS 42521726 42521938 42521726 ID=cds.MS.gene07674.t1;Parent=MS.gene07674.t1
chr4.1 exon 42522253 42522315 42522253 ID=MS.gene07674.t1.exon3;Parent=MS.gene07674.t1
chr4.1 CDS 42522253 42522315 42522253 ID=cds.MS.gene07674.t1;Parent=MS.gene07674.t1
Gene Sequence

>MS.gene07674

ATGGCGTGCAAAGATGAAAAACATGATCGATTAAGCAATCTCTCAGACGATTTGATTTGTCATATTCTGTCTTTCCTATCAACAAAAGAGTCTTATAGAACATGTGTTCTATCCACAAGATGGGAGTCGATATGCACCAAGATTCCCAACCTTCACTTTCAATTACCAGAGATTTCAGACCCTGAGTTGTCCAAAGAAATCAAATTTGTGAATGTTGCTCTCCTAAGACGCACCGAAAATATAAGAAAACTAAGTCTTGATAGCAACAAAGGCTGCCAGCCACATGATGTACACATGTGGGTTTCAAAGGCATTAGATCTCAAGGTCCAGAAACTTGACTTGGATCTGTTTCTCCATGAAAAAATCTTATTGCCACTTAGGCTTTTCACGTGTGAGTCACTCGTGGTGTTAAAATTGCGGGGCAGAATTCAACCAACACTCAATTCTTCTTTTCATGTATATTTACCGTCACTAAAGATTCTTCATCTCCGTGAAACTGTGGCGTATTCTATCTTTGATGATCGTAGAGAGTATGATCTAATAATTTTCTATCTGGTTGTCCACATCTAGAGGAACTTCTTTTAGCCGAGACCTTTGCATTACCTATCAACACCTCTTTTCACTTATTGAAGAGATTATTCCTTACTTTATATATGCCTACTTCGGTTATTAAGTGTTGTCCACTACAAATTAATGCCCCTTCACTTGAAGTTCTTTCTATTGTTGATTTTTCATTGGCTCCTAGAAAATATGACTTTACAAACTTATCCACTGTTGATGAAGCTGCTCTTTGCATTTGTAAGCGTCCGGATTTTAACAATTTGTATACACTCTTGAAAGGACTTTCTAATGTCAAAACATTGGCTCTCGGTGCTAAAACTTTTCATGTAAGTTTCATAAATATGTTTCTTTTTTAATTACAAGTTCCAAATTTAGTCACCCATATGCAATTCTATTCTCATTTTATAATTTTGTTATGCAGTTTCTCAGCATGGAGGACAAACTTGACAACTTACATCTCCTCACTTTTCACAATTTATTATATTTAACGATTGAAATATCTGAGAATTGCAGTTGGAATATGCTAGTCAGCTTTCTACAGAATGCTCCAAAACTTAAGGATCTTGCAATTATGGTGTGTATGATGTTCTTTTAACTATATTTGCACAATATTTTTTTTTTAAAAGATGAAATATAATAGGCTCAAAAGTCTGGCAGTAGAAGAGCAAAGACTTGGCAAAATATACATGGAGTTTGCAAATAA

Protein sequence

>MS.gene07674.t1

MACKDEKHDRLSNLSDDLICHILSFLSTKESYRTCVLSTRWESICTKIPNLHFQLPEISDPELSKEIKFVNVALLRRTENIRKLSLDSNKGCQPHDVHMWVSKALDLKCCPLQINAPSLEVLSIVDFSLAPRKYDFTNLSTVDEAALCICKRPDFNNLYTLLKGLSNVKTLALGAKTFHAQKSGSRRAKTWQNIHGVCK