AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069712


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069712.t1 MTR_1g082420 92.667 150 7 1 1 150 144 289 6.52e-95 276
MS.gene069712.t1 MTR_1g082420 98.438 64 1 0 1 64 144 207 2.55e-39 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069712.t1 AT1G54780 81.333 150 24 1 1 150 140 285 2.89e-87 257

Find 34 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTTTGCGGACCAAATTT+TGG 0.287868 1.4:-56192027 MS.gene069712:CDS
TATCAATTGTCAATACATAA+AGG 0.302146 1.4:-56190579 MS.gene069712:CDS
GATACCAACGCTCCAAAATT+TGG 0.302536 1.4:+56192015 None:intergenic
TCTTGTAGTTGGAGGTTTGT+TGG 0.348630 1.4:-56190872 MS.gene069712:CDS
ATCAATTGTCAATACATAAA+GGG 0.365525 1.4:-56190578 MS.gene069712:CDS
ACTGTTTCAGAGAACCTTCC+AGG 0.376813 1.4:-56191873 MS.gene069712:intron
CAGAAAGAAGGAGCAGTTAC+TGG 0.391425 1.4:-56191942 MS.gene069712:CDS
CAATTCTCTCTTGTAGTTGG+AGG 0.420228 1.4:-56190880 MS.gene069712:CDS
TATTGCATTTGTTGTTCCTA+TGG 0.427254 1.4:-56190848 MS.gene069712:intron
CGACTAGTTGCTGCCATTGA+CGG 0.431818 1.4:-56190991 MS.gene069712:CDS
ACCGAGGGACCACCAGGATC+TGG 0.448423 1.4:+56190964 None:intergenic
GGACAATTCTCTCTTGTAGT+TGG 0.450595 1.4:-56190883 MS.gene069712:CDS
ACGTTTGTCGTCTTTAACCG+AGG 0.492907 1.4:+56190948 None:intergenic
TCCAGATCCTGGTGGTCCCT+CGG 0.499224 1.4:-56190965 MS.gene069712:CDS
TTGGTATCCTTCTGTGGAAG+AGG 0.505336 1.4:-56192000 MS.gene069712:CDS
TTTGTATGCAGTATTGGCTA+CGG 0.505886 1.4:-56191052 MS.gene069712:intron
TCTTTAACCGAGGGACCACC+AGG 0.532906 1.4:+56190958 None:intergenic
ATTGACGGACTTCCAGATCC+TGG 0.535082 1.4:-56190976 MS.gene069712:CDS
AGGATCTGGAAGTCCGTCAA+TGG 0.539431 1.4:+56190978 None:intergenic
TCATTACCCTCTTCCACAGA+AGG 0.547276 1.4:+56191993 None:intergenic
CCTACGGCTTGAACAAAAGC+AGG 0.557352 1.4:+56191915 None:intergenic
CTTGTAACAAGTCAGAAAGA+AGG 0.568574 1.4:-56191954 MS.gene069712:CDS
GGGTAATGACAAGGGTGTTG+TGG 0.576837 1.4:-56191979 MS.gene069712:CDS
CGTTTGTCGTCTTTAACCGA+GGG 0.605535 1.4:+56190949 None:intergenic
GGAGCGTTGGTATCCTTCTG+TGG 0.606556 1.4:-56192006 MS.gene069712:CDS
GAAGAGACAGAAGAAAAGCG+TGG 0.608619 1.4:-56190904 MS.gene069712:CDS
TGGTATCCTTCTGTGGAAGA+GGG 0.615864 1.4:-56191999 MS.gene069712:CDS
TGAGTCTAACTTCAAAACAA+AGG 0.628354 1.4:-56190926 MS.gene069712:CDS
TGTGGAAGAGGGTAATGACA+AGG 0.647686 1.4:-56191988 MS.gene069712:CDS
GCCATTAGGAAGTACCTGGA+AGG 0.650563 1.4:+56191859 None:intergenic
AAAGAAGGAGCAGTTACTGG+TGG 0.670414 1.4:-56191939 MS.gene069712:CDS
GTGGAAGAGGGTAATGACAA+GGG 0.686921 1.4:-56191987 MS.gene069712:CDS
GACGGACTTCCAGATCCTGG+TGG 0.696278 1.4:-56190973 MS.gene069712:CDS
TCTCTGAAACAGTAGCATCG+AGG 0.748886 1.4:+56191883 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATCCATTATCTTATTTTT+TGG + chr1.4:56191757-56191776 None:intergenic 15.0%
!! AACTCTTCTTAACAAAAAAA+GGG + chr1.4:56191095-56191114 None:intergenic 20.0%
!! AATAATCCAACAAACATATT+TGG + chr1.4:56191922-56191941 None:intergenic 20.0%
!! ATAATCCAACAAACATATTT+GGG + chr1.4:56191921-56191940 None:intergenic 20.0%
!! ATCAATTGTCAATACATAAA+GGG - chr1.4:56191980-56191999 MS.gene069712:CDS 20.0%
!! GAAATTAAAGAGTGATAATT+TGG + chr1.4:56191801-56191820 None:intergenic 20.0%
!! TATCAATTGTCAATACATAA+AGG - chr1.4:56191979-56191998 MS.gene069712:CDS 20.0%
!! TTTCTTGAAAAATAAATGCA+AGG - chr1.4:56191016-56191035 MS.gene069712:CDS 20.0%
! CAACTCTTCTTAACAAAAAA+AGG + chr1.4:56191096-56191115 None:intergenic 25.0%
! GTAGCCAAAAAATAAGATAA+TGG - chr1.4:56191750-56191769 MS.gene069712:intron 25.0%
! TAATTCCCAAATATGTTTGT+TGG - chr1.4:56191913-56191932 MS.gene069712:CDS 25.0%
! TACATACTTCAGTTTAAACA+AGG - chr1.4:56191309-56191328 MS.gene069712:intron 25.0%
! TGATTATTTATGTCTGTGAA+AGG - chr1.4:56190808-56190827 MS.gene069712:intron 25.0%
! TTGTTTAAACTGAAGTATGT+AGG + chr1.4:56191310-56191329 None:intergenic 25.0%
!! AAGCAACTTTGTTATGAATA+TGG - chr1.4:56190929-56190948 MS.gene069712:CDS 25.0%
!!! ATTGTTTTTCTGTAGCATAT+TGG - chr1.4:56190741-56190760 MS.gene069712:intron 25.0%
!!! CAAATAAGATCAGAGTTTTT+TGG - chr1.4:56191880-56191899 MS.gene069712:CDS 25.0%
!!! TTGTTTTTCTGTAGCATATT+GGG - chr1.4:56190742-56190761 MS.gene069712:intron 25.0%
!!! TTTTTTCTCACCAACAAATA+TGG - chr1.4:56190862-56190881 MS.gene069712:CDS 25.0%
!!! TTTTTTGTTAAGAAGAGTTG+AGG - chr1.4:56191096-56191115 MS.gene069712:intron 25.0%
ACTCAATTATAACATGAGCT+TGG + chr1.4:56191847-56191866 None:intergenic 30.0%
ACTGAGTATATAGTAAAGGA+CGG - chr1.4:56190968-56190987 MS.gene069712:CDS 30.0%
ATACAGCTACTAAATGGATA+GGG + chr1.4:56191267-56191286 None:intergenic 30.0%
ATGCACTGAGTATATAGTAA+AGG - chr1.4:56190964-56190983 MS.gene069712:CDS 30.0%
CAACATATACAGCTACTAAA+TGG + chr1.4:56191273-56191292 None:intergenic 30.0%
CATATGTTCATAAACTGTGA+AGG - chr1.4:56190906-56190925 MS.gene069712:CDS 30.0%
TAAGCATAGTATTGTACCAT+AGG + chr1.4:56191729-56191748 None:intergenic 30.0%
TATACAGCTACTAAATGGAT+AGG + chr1.4:56191268-56191287 None:intergenic 30.0%
TCATCATGATCATTGATCAT+TGG + chr1.4:56191168-56191187 None:intergenic 30.0%
TGAGTCTAACTTCAAAACAA+AGG - chr1.4:56191632-56191651 MS.gene069712:intron 30.0%
TTTAAACTGAAGTATGTAGG+CGG + chr1.4:56191307-56191326 None:intergenic 30.0%
! TGTTTTTCTGTAGCATATTG+GGG - chr1.4:56190743-56190762 MS.gene069712:intron 30.0%
!! AGCAGTTGTGATCATTTTTA+GGG - chr1.4:56191454-56191473 MS.gene069712:intron 30.0%
!! TAGCAGTTGTGATCATTTTT+AGG - chr1.4:56191453-56191472 MS.gene069712:intron 30.0%
!! TATTGCATTTGTTGTTCCTA+TGG - chr1.4:56191710-56191729 MS.gene069712:intron 30.0%
AAGGATTTACAAGACTGTCT+TGG - chr1.4:56190827-56190846 MS.gene069712:intron 35.0%
AGTAGCTGTATATGTTGTCA+AGG - chr1.4:56191276-56191295 MS.gene069712:intron 35.0%
CATGTCCATCATTATCGTTA+TGG + chr1.4:56191191-56191210 None:intergenic 35.0%
CTTCAGTTTAAACAAGGCAA+CGG - chr1.4:56191315-56191334 MS.gene069712:intron 35.0%
CTTGTAACAAGTCAGAAAGA+AGG - chr1.4:56190604-56190623 MS.gene069712:intron 35.0%
GATGACCATAACGATAATGA+TGG - chr1.4:56191183-56191202 MS.gene069712:intron 35.0%
GTAAAGGACGGATGATAATT+TGG - chr1.4:56190980-56190999 MS.gene069712:CDS 35.0%
GTAACTTCAGAAGACATACA+TGG + chr1.4:56191423-56191442 None:intergenic 35.0%
TGCATACAAAAACCAGACAA+TGG + chr1.4:56191496-56191515 None:intergenic 35.0%
TGCATCAAGTGCATAAAAAC+TGG + chr1.4:56191953-56191972 None:intergenic 35.0%
TTCACACTTGCCATATTTGT+TGG + chr1.4:56190875-56190894 None:intergenic 35.0%
! ATCGAGGATATTTTCTCCTA+CGG + chr1.4:56190662-56190681 None:intergenic 35.0%
!! CTGGTTTTTGTATGCAGTAT+TGG - chr1.4:56191500-56191519 MS.gene069712:intron 35.0%
!! GAATTGTTTTGTCAGCATGT+GGG - chr1.4:56191052-56191071 MS.gene069712:intron 35.0%
!! GCAGTTGTGATCATTTTTAG+GGG - chr1.4:56191455-56191474 MS.gene069712:intron 35.0%
!! TGAATTGTTTTGTCAGCATG+TGG - chr1.4:56191051-56191070 MS.gene069712:intron 35.0%
!! TTTGTATGCAGTATTGGCTA+CGG - chr1.4:56191506-56191525 MS.gene069712:intron 35.0%
!!! ATCCCTTTTTAGCTAGCATA+CGG - chr1.4:56191240-56191259 MS.gene069712:intron 35.0%
AACACACTACTACCATTGTC+TGG - chr1.4:56191481-56191500 MS.gene069712:intron 40.0%
AGTTGAGGTTGTGCTTTATC+AGG - chr1.4:56191111-56191130 MS.gene069712:intron 40.0%
ATCCAAATCCACTAGCCATT+AGG + chr1.4:56190716-56190735 None:intergenic 40.0%
CAATTCTCTCTTGTAGTTGG+AGG - chr1.4:56191678-56191697 MS.gene069712:intron 40.0%
GACCGTATGCTAGCTAAAAA+GGG + chr1.4:56191245-56191264 None:intergenic 40.0%
GATACCAACGCTCCAAAATT+TGG + chr1.4:56190546-56190565 None:intergenic 40.0%
GGACAATTCTCTCTTGTAGT+TGG - chr1.4:56191675-56191694 MS.gene069712:intron 40.0%
GTTGAGGTTGTGCTTTATCA+GGG - chr1.4:56191112-56191131 MS.gene069712:intron 40.0%
TCTTGTAGTTGGAGGTTTGT+TGG - chr1.4:56191686-56191705 MS.gene069712:intron 40.0%
TTCAGCAGGGTGATAAACTA+GGG - chr1.4:56191341-56191360 MS.gene069712:intron 40.0%
TTGAGGTTGTGCTTTATCAG+GGG - chr1.4:56191113-56191132 MS.gene069712:intron 40.0%
TTTCAGCAGGGTGATAAACT+AGG - chr1.4:56191340-56191359 MS.gene069712:intron 40.0%
! TGAGTTTGCGGACCAAATTT+TGG - chr1.4:56190531-56190550 MS.gene069712:CDS 40.0%
!! TTCCTAATGGCTAGTGGATT+TGG - chr1.4:56190711-56190730 MS.gene069712:intron 40.0%
AAAGAAGGAGCAGTTACTGG+TGG - chr1.4:56190619-56190638 MS.gene069712:intron 45.0%
ACCTTCCAGGTACTTCCTAA+TGG - chr1.4:56190698-56190717 MS.gene069712:intron 45.0%
ACGTTTGTCGTCTTTAACCG+AGG + chr1.4:56191613-56191632 None:intergenic 45.0%
ACTGTTTCAGAGAACCTTCC+AGG - chr1.4:56190685-56190704 MS.gene069712:intron 45.0%
AGGTACTTCCTAATGGCTAG+TGG - chr1.4:56190705-56190724 MS.gene069712:intron 45.0%
CAGAAAGAAGGAGCAGTTAC+TGG - chr1.4:56190616-56190635 MS.gene069712:intron 45.0%
CGTTTGTCGTCTTTAACCGA+GGG + chr1.4:56191612-56191631 None:intergenic 45.0%
GAAGAGACAGAAGAAAAGCG+TGG - chr1.4:56191654-56191673 MS.gene069712:intron 45.0%
GGACCGTATGCTAGCTAAAA+AGG + chr1.4:56191246-56191265 None:intergenic 45.0%
GTGGAAGAGGGTAATGACAA+GGG - chr1.4:56190571-56190590 MS.gene069712:CDS 45.0%
TCATTACCCTCTTCCACAGA+AGG + chr1.4:56190568-56190587 None:intergenic 45.0%
TGGTATCCTTCTGTGGAAGA+GGG - chr1.4:56190559-56190578 MS.gene069712:CDS 45.0%
TGTGGAAGAGGGTAATGACA+AGG - chr1.4:56190570-56190589 MS.gene069712:CDS 45.0%
TTGGTATCCTTCTGTGGAAG+AGG - chr1.4:56190558-56190577 MS.gene069712:CDS 45.0%
!! AAGGCAACGGATTTTCAGCA+GGG - chr1.4:56191328-56191347 MS.gene069712:intron 45.0%
!! ACTAGCCATTAGGAAGTACC+TGG + chr1.4:56190706-56190725 None:intergenic 45.0%
!! TCTCTGAAACAGTAGCATCG+AGG + chr1.4:56190678-56190697 None:intergenic 45.0%
!!! CGCTGATGCTTTTGAGTTTG+CGG - chr1.4:56190519-56190538 MS.gene069712:CDS 45.0%
AGGATCTGGAAGTCCGTCAA+TGG + chr1.4:56191583-56191602 None:intergenic 50.0%
CCTACGGCTTGAACAAAAGC+AGG + chr1.4:56190646-56190665 None:intergenic 50.0%
! CGACTAGTTGCTGCCATTGA+CGG - chr1.4:56191567-56191586 MS.gene069712:intron 50.0%
!! ATTGACGGACTTCCAGATCC+TGG - chr1.4:56191582-56191601 MS.gene069712:intron 50.0%
!! CAAGGCAACGGATTTTCAGC+AGG - chr1.4:56191327-56191346 MS.gene069712:intron 50.0%
!! CCTGCTTTTGTTCAAGCCGT+AGG - chr1.4:56190643-56190662 MS.gene069712:intron 50.0%
!! GCCATTAGGAAGTACCTGGA+AGG + chr1.4:56190702-56190721 None:intergenic 50.0%
!! GGGTAATGACAAGGGTGTTG+TGG - chr1.4:56190579-56190598 MS.gene069712:CDS 50.0%
!!! CGGACCAAATTTTGGAGCGT+TGG - chr1.4:56190539-56190558 MS.gene069712:CDS 50.0%
TCTTTAACCGAGGGACCACC+AGG + chr1.4:56191603-56191622 None:intergenic 55.0%
!! GGAGCGTTGGTATCCTTCTG+TGG - chr1.4:56190552-56190571 MS.gene069712:CDS 55.0%
TCCAGATCCTGGTGGTCCCT+CGG - chr1.4:56191593-56191612 MS.gene069712:intron 60.0%
! GACGGACTTCCAGATCCTGG+TGG - chr1.4:56191585-56191604 MS.gene069712:intron 60.0%
ACCGAGGGACCACCAGGATC+TGG + chr1.4:56191597-56191616 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 56190514 56192066 56190514 ID=MS.gene069712
chr1.4 mRNA 56190514 56192066 56190514 ID=MS.gene069712.t1;Parent=MS.gene069712
chr1.4 exon 56191874 56192066 56191874 ID=MS.gene069712.t1.exon1;Parent=MS.gene069712.t1
chr1.4 CDS 56191874 56192066 56191874 ID=cds.MS.gene069712.t1;Parent=MS.gene069712.t1
chr1.4 exon 56190849 56191063 56190849 ID=MS.gene069712.t1.exon2;Parent=MS.gene069712.t1
chr1.4 CDS 56190849 56191063 56190849 ID=cds.MS.gene069712.t1;Parent=MS.gene069712.t1
chr1.4 exon 56190514 56190606 56190514 ID=MS.gene069712.t1.exon3;Parent=MS.gene069712.t1
chr1.4 CDS 56190514 56190606 56190514 ID=cds.MS.gene069712.t1;Parent=MS.gene069712.t1
Gene Sequence

>MS.gene069712

AGTAACGCTGATGCTTTTGAGTTTGCGGACCAAATTTTGGAGCGTTGGTATCCTTCTGTGGAAGAGGGTAATGACAAGGGTGTTGTGGTTCTTGTAACAAGTCAGAAAGAAGGAGCAGTTACTGGTGGACCTGCTTTTGTTCAAGCCGTAGGAGAAAATATCCTCGATGCTACTGTTTCAGAGAACCTTCCAGGTACTTCCTAATGGCTAGTGGATTTGGATTCTTTATTGTTTTTCTGTAGCATATTGGGGAGAGAATTACATTATTTGTATCACATAAGAACTTAACTATCATGATTATTTATGTCTGTGAAAGGATTTACAAGACTGTCTTGGAGTTTTTTCTTCTTTTTTCTCACCAACAAATATGGCAAGTGTGAACTGCTGATTGTCATATGTTCATAAACTGTGAAGGAAGCAACTTTGTTATGAATATGGTCATAACATGTCATGCACTGAGTATATAGTAAAGGACGGATGATAATTTGGATTCAAGTATTTTTTTCTTGAAAAATAAATGCAAGGTCAACGACAATGTGAATTGTTTTGTCAGCATGTGGGTGAATCACTTGTTATGACCCTTTTTTTGTTAAGAAGAGTTGAGGTTGTGCTTTATCAGGGGCACTTTCAAGAAGCAAGATTGCAGACACTCCAATGATCAATGATCATGATGACCATAACGATAATGATGGACATGTTTGCATTTTTAGAAATCAACAAATGATAATCCCTTTTTAGCTAGCATACGGTCCCTATCCATTTAGTAGCTGTATATGTTGTCAAGGCTTATCCGCCTACATACTTCAGTTTAAACAAGGCAACGGATTTTCAGCAGGGTGATAAACTAGGGTACAACTTTTGAATGCACATACTATTGATAGTCTGCAAATTTGTATTATTTATAAACCATGTATGTCTTCTGAAGTTACAATCAATAAGTAGCAGTTGTGATCATTTTTAGGGGCACAACACACTACTACCATTGTCTGGTTTTTGTATGCAGTATTGGCTACGGATGAGAAGTACAATGAAGCAATTTATAGCACTGCTAAACGACTAGTTGCTGCCATTGACGGACTTCCAGATCCTGGTGGTCCCTCGGTTAAAGACGACAAACGTGAGTCTAACTTCAAAACAAAGGAAGAGACAGAAGAAAAGCGTGGACAATTCTCTCTTGTAGTTGGAGGTTTGTTGGTTATTGCATTTGTTGTTCCTATGGTACAATACTATGCTTATGTAGCCAAAAAATAAGATAATGGATATACAACTAGATGAATCTCTCTTCCAAATTATCACTCTTTAATTTCAGATGTATAATGCACAATTGTAACCAAGCTCATGTTATAATTGAGTTTGAGAAAAATGACAAATAAGATCAGAGTTTTTTGGCTTGTAAATGTAATTCCCAAATATGTTTGTTGGATTATTTTTACATGCCAGTTTTTATGCACTTGATGCAGACATTTATCAATTGTCAATACATAAAGGGTTTGCAAAAGAAACAGTTTTATGCTATTACGCATACTGTGTCATACAACTCTAATCAAGCATAA

Protein sequence

>MS.gene069712.t1

SNADAFEFADQILERWYPSVEEGNDKGVVVLVTSQKEGAVTGGPAFVQAVGENILDATVSENLPVLATDEKYNEAIYSTAKRLVAAIDGLPDPGGPSVKDDKRESNFKTKEETEEKRGQFSLVVGGLLVIAFVVPMTFINCQYIKGLQKKQFYAITHTVSYNSNQA