Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30834.t1 | XP_013452555.1 | 95 | 242 | 11 | 1 | 1 | 241 | 1 | 242 | 4.80E-122 | 447.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30834.t1 | G7ZX37 | 95.0 | 242 | 11 | 1 | 1 | 241 | 1 | 242 | 3.5e-122 | 447.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene30834 | 0.851561 | 8.19E-61 | -1.69E-46 |
| MS.gene049171 | MS.gene30834 | 0.813322 | 2.71E-51 | -1.69E-46 |
| MS.gene049173 | MS.gene30834 | 0.811699 | 6.14E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene30834 | 0.809275 | 2.05E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene30834 | 0.822806 | 1.94E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene30834 | 0.804472 | 2.13E-49 | -1.69E-46 |
| MS.gene049427 | MS.gene30834 | 0.837835 | 4.11E-57 | -1.69E-46 |
| MS.gene049488 | MS.gene30834 | 0.834243 | 3.35E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene30834 | 0.86958 | 2.78E-66 | -1.69E-46 |
| MS.gene049661 | MS.gene30834 | 0.814039 | 1.89E-51 | -1.69E-46 |
| MS.gene049662 | MS.gene30834 | 0.803107 | 4.08E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene30834 | 0.84014 | 1.04E-57 | -1.69E-46 |
| MS.gene050397 | MS.gene30834 | 0.816029 | 6.83E-52 | -1.69E-46 |
| MS.gene050400 | MS.gene30834 | 0.811581 | 6.52E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene30834 | 0.80578 | 1.13E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene30834 | 0.803792 | 2.94E-49 | -1.69E-46 |
| MS.gene050538 | MS.gene30834 | 0.806823 | 6.82E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene30834 | 0.855085 | 7.99E-62 | -1.69E-46 |
| MS.gene050625 | MS.gene30834 | 0.835519 | 1.60E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene30834 | 0.882677 | 8.52E-71 | -1.69E-46 |
| MS.gene050747 | MS.gene30834 | 0.834996 | 2.17E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene30834 | 0.841869 | 3.66E-58 | -1.69E-46 |
| MS.gene05081 | MS.gene30834 | 0.815135 | 1.08E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene30834 | 0.822799 | 1.95E-53 | -1.69E-46 |
| MS.gene05105 | MS.gene30834 | 0.892766 | 1.17E-74 | -1.69E-46 |
| MS.gene051360 | MS.gene30834 | 0.818559 | 1.84E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene30834 | 0.84158 | 4.36E-58 | -1.69E-46 |
| MS.gene051525 | MS.gene30834 | 0.844115 | 9.23E-59 | -1.69E-46 |
| MS.gene051582 | MS.gene30834 | 0.80645 | 8.18E-50 | -1.69E-46 |
| MS.gene051605 | MS.gene30834 | 0.832177 | 1.10E-55 | -1.69E-46 |
| MS.gene051666 | MS.gene30834 | 0.879749 | 9.66E-70 | -1.69E-46 |
| MS.gene051692 | MS.gene30834 | 0.868039 | 8.78E-66 | -1.69E-46 |
| MS.gene051734 | MS.gene30834 | 0.802125 | 6.51E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene30834 | 0.842766 | 2.12E-58 | -1.69E-46 |
| MS.gene051858 | MS.gene30834 | 0.832366 | 9.85E-56 | -1.69E-46 |
| MS.gene052011 | MS.gene30834 | -0.800416 | 1.46E-48 | -1.69E-46 |
| MS.gene052134 | MS.gene30834 | 0.831615 | 1.51E-55 | -1.69E-46 |
| MS.gene052135 | MS.gene30834 | 0.836844 | 7.37E-57 | -1.69E-46 |
| MS.gene052136 | MS.gene30834 | 0.836322 | 1.00E-56 | -1.69E-46 |
| MS.gene052188 | MS.gene30834 | 0.8394 | 1.62E-57 | -1.69E-46 |
| MS.gene052253 | MS.gene30834 | 0.864953 | 8.39E-65 | -1.69E-46 |
| MS.gene052310 | MS.gene30834 | 0.842861 | 2.00E-58 | -1.69E-46 |
| MS.gene052322 | MS.gene30834 | 0.844023 | 9.77E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene30834 | 0.845346 | 4.30E-59 | -1.69E-46 |
| MS.gene052346 | MS.gene30834 | 0.828898 | 6.96E-55 | -1.69E-46 |
| MS.gene052414 | MS.gene30834 | 0.883501 | 4.25E-71 | -1.69E-46 |
| MS.gene052421 | MS.gene30834 | 0.836631 | 8.36E-57 | -1.69E-46 |
| MS.gene052481 | MS.gene30834 | 0.905456 | 4.21E-80 | -1.69E-46 |
| MS.gene052484 | MS.gene30834 | 0.87347 | 1.43E-67 | -1.69E-46 |
| MS.gene052485 | MS.gene30834 | 0.860068 | 2.67E-63 | -1.69E-46 |
| MS.gene052501 | MS.gene30834 | 0.874615 | 5.88E-68 | -1.69E-46 |
| MS.gene052503 | MS.gene30834 | 0.837246 | 5.82E-57 | -1.69E-46 |
| MS.gene052505 | MS.gene30834 | 0.8623 | 5.59E-64 | -1.69E-46 |
| MS.gene052615 | MS.gene30834 | 0.817091 | 3.95E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene30834 | 0.820566 | 6.41E-53 | -1.69E-46 |
| MS.gene052825 | MS.gene30834 | 0.823217 | 1.56E-53 | -1.69E-46 |
| MS.gene052982 | MS.gene30834 | 0.874484 | 6.52E-68 | -1.69E-46 |
| MS.gene053103 | MS.gene30834 | 0.863224 | 2.90E-64 | -1.69E-46 |
| MS.gene053104 | MS.gene30834 | 0.851461 | 8.74E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene30834 | 0.826972 | 2.02E-54 | -1.69E-46 |
| MS.gene053209 | MS.gene30834 | 0.840646 | 7.67E-58 | -1.69E-46 |
| MS.gene053213 | MS.gene30834 | 0.816677 | 4.89E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene30834 | 0.805135 | 1.54E-49 | -1.69E-46 |
| MS.gene053322 | MS.gene30834 | 0.80648 | 8.06E-50 | -1.69E-46 |
| MS.gene053436 | MS.gene30834 | 0.80366 | 3.14E-49 | -1.69E-46 |
| MS.gene053449 | MS.gene30834 | 0.852126 | 5.66E-61 | -1.69E-46 |
| MS.gene053574 | MS.gene30834 | 0.838739 | 2.40E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene30834 | 0.82667 | 2.39E-54 | -1.69E-46 |
| MS.gene053758 | MS.gene30834 | 0.801539 | 8.59E-49 | -1.69E-46 |
| MS.gene053873 | MS.gene30834 | 0.81317 | 2.93E-51 | -1.69E-46 |
| MS.gene053912 | MS.gene30834 | 0.843667 | 1.22E-58 | -1.69E-46 |
| MS.gene054600 | MS.gene30834 | 0.815728 | 7.97E-52 | -1.69E-46 |
| MS.gene054927 | MS.gene30834 | 0.848258 | 6.87E-60 | -1.69E-46 |
| MS.gene054955 | MS.gene30834 | 0.804081 | 2.56E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene30834 | 0.827067 | 1.92E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene30834 | 0.896528 | 3.38E-76 | -1.69E-46 |
| MS.gene055206 | MS.gene30834 | 0.844161 | 8.97E-59 | -1.69E-46 |
| MS.gene055289 | MS.gene30834 | 0.820768 | 5.76E-53 | -1.69E-46 |
| MS.gene055412 | MS.gene30834 | 0.801262 | 9.78E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene30834 | 0.800393 | 1.47E-48 | -1.69E-46 |
| MS.gene055539 | MS.gene30834 | 0.82625 | 3.01E-54 | -1.69E-46 |
| MS.gene05553 | MS.gene30834 | 0.806645 | 7.44E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene30834 | 0.851043 | 1.15E-60 | -1.69E-46 |
| MS.gene055741 | MS.gene30834 | 0.817621 | 3.00E-52 | -1.69E-46 |
| MS.gene055744 | MS.gene30834 | 0.824887 | 6.33E-54 | -1.69E-46 |
| MS.gene055909 | MS.gene30834 | 0.801082 | 1.06E-48 | -1.69E-46 |
| MS.gene055911 | MS.gene30834 | 0.802289 | 6.02E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene30834 | 0.814489 | 1.50E-51 | -1.69E-46 |
| MS.gene056166 | MS.gene30834 | 0.834458 | 2.96E-56 | -1.69E-46 |
| MS.gene056191 | MS.gene30834 | 0.820217 | 7.71E-53 | -1.69E-46 |
| MS.gene056374 | MS.gene30834 | 0.801337 | 9.45E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene30834 | 0.813826 | 2.10E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene30834 | 0.806204 | 9.22E-50 | -1.69E-46 |
| MS.gene056448 | MS.gene30834 | 0.801604 | 8.32E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene30834 | 0.869565 | 2.82E-66 | -1.69E-46 |
| MS.gene05664 | MS.gene30834 | 0.85179 | 7.06E-61 | -1.69E-46 |
| MS.gene05666 | MS.gene30834 | 0.810666 | 1.03E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene30834 | 0.81206 | 5.13E-51 | -1.69E-46 |
| MS.gene056877 | MS.gene30834 | 0.822086 | 2.86E-53 | -1.69E-46 |
| MS.gene056897 | MS.gene30834 | 0.810792 | 9.66E-51 | -1.69E-46 |
| MS.gene056946 | MS.gene30834 | 0.816545 | 5.23E-52 | -1.69E-46 |
| MS.gene05702 | MS.gene30834 | 0.83042 | 2.97E-55 | -1.69E-46 |
| MS.gene057048 | MS.gene30834 | 0.815261 | 1.01E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene30834 | 0.827913 | 1.20E-54 | -1.69E-46 |
| MS.gene057124 | MS.gene30834 | 0.839267 | 1.76E-57 | -1.69E-46 |
| MS.gene057146 | MS.gene30834 | 0.83552 | 1.60E-56 | -1.69E-46 |
| MS.gene057196 | MS.gene30834 | 0.807762 | 4.31E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene30834 | 0.821777 | 3.37E-53 | -1.69E-46 |
| MS.gene057330 | MS.gene30834 | 0.808608 | 2.85E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene30834 | 0.825659 | 4.16E-54 | -1.69E-46 |
| MS.gene057332 | MS.gene30834 | 0.806847 | 6.74E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene30834 | 0.845522 | 3.85E-59 | -1.69E-46 |
| MS.gene057658 | MS.gene30834 | 0.838261 | 3.19E-57 | -1.69E-46 |
| MS.gene057734 | MS.gene30834 | 0.855364 | 6.63E-62 | -1.69E-46 |
| MS.gene057749 | MS.gene30834 | 0.875021 | 4.28E-68 | -1.69E-46 |
| MS.gene057823 | MS.gene30834 | 0.864136 | 1.51E-64 | -1.69E-46 |
| MS.gene057825 | MS.gene30834 | 0.816026 | 6.84E-52 | -1.69E-46 |
| MS.gene057856 | MS.gene30834 | 0.843432 | 1.41E-58 | -1.69E-46 |
| MS.gene058006 | MS.gene30834 | 0.80432 | 2.29E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene30834 | 0.855998 | 4.33E-62 | -1.69E-46 |
| MS.gene058019 | MS.gene30834 | 0.858268 | 9.26E-63 | -1.69E-46 |
| MS.gene058082 | MS.gene30834 | 0.801612 | 8.29E-49 | -1.69E-46 |
| MS.gene058233 | MS.gene30834 | 0.809511 | 1.83E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene30834 | 0.873134 | 1.86E-67 | -1.69E-46 |
| MS.gene058419 | MS.gene30834 | 0.807277 | 5.47E-50 | -1.69E-46 |
| MS.gene058444 | MS.gene30834 | 0.888192 | 7.34E-73 | -1.69E-46 |
| MS.gene058544 | MS.gene30834 | 0.805891 | 1.07E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene30834 | 0.823334 | 1.46E-53 | -1.69E-46 |
| MS.gene058700 | MS.gene30834 | 0.849492 | 3.12E-60 | -1.69E-46 |
| MS.gene058838 | MS.gene30834 | 0.844502 | 7.27E-59 | -1.69E-46 |
| MS.gene058928 | MS.gene30834 | 0.832574 | 8.74E-56 | -1.69E-46 |
| MS.gene058948 | MS.gene30834 | 0.821362 | 4.21E-53 | -1.69E-46 |
| MS.gene058995 | MS.gene30834 | 0.814673 | 1.37E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene30834 | 0.811686 | 6.18E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene30834 | 0.816701 | 4.83E-52 | -1.69E-46 |
| MS.gene059141 | MS.gene30834 | 0.838617 | 2.59E-57 | -1.69E-46 |
| MS.gene05934 | MS.gene30834 | 0.83086 | 2.31E-55 | -1.69E-46 |
| MS.gene059366 | MS.gene30834 | 0.817397 | 3.37E-52 | -1.69E-46 |
| MS.gene059420 | MS.gene30834 | 0.820985 | 5.13E-53 | -1.69E-46 |
| MS.gene059455 | MS.gene30834 | 0.864169 | 1.48E-64 | -1.69E-46 |
| MS.gene059536 | MS.gene30834 | 0.856719 | 2.66E-62 | -1.69E-46 |
| MS.gene059597 | MS.gene30834 | 0.827734 | 1.33E-54 | -1.69E-46 |
| MS.gene059667 | MS.gene30834 | 0.80161 | 8.30E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene30834 | 0.828352 | 9.43E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene30834 | 0.873577 | 1.32E-67 | -1.69E-46 |
| MS.gene059692 | MS.gene30834 | 0.872071 | 4.21E-67 | -1.69E-46 |
| MS.gene059695 | MS.gene30834 | 0.874845 | 4.91E-68 | -1.69E-46 |
| MS.gene059711 | MS.gene30834 | 0.802628 | 5.13E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene30834 | 0.816863 | 4.44E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene30834 | 0.802286 | 6.03E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene30834 | 0.828543 | 8.48E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene30834 | 0.88642 | 3.47E-72 | -1.69E-46 |
| MS.gene060469 | MS.gene30834 | 0.812055 | 5.14E-51 | -1.69E-46 |
| MS.gene060485 | MS.gene30834 | 0.810705 | 1.01E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene30834 | 0.854887 | 9.12E-62 | -1.69E-46 |
| MS.gene060818 | MS.gene30834 | 0.808741 | 2.67E-50 | -1.69E-46 |
| MS.gene060835 | MS.gene30834 | 0.84009 | 1.07E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene30834 | 0.862493 | 4.87E-64 | -1.69E-46 |
| MS.gene06092 | MS.gene30834 | 0.818414 | 1.98E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene30834 | 0.82885 | 7.15E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene30834 | 0.820266 | 7.51E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene30834 | 0.809476 | 1.86E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene30834 | 0.802809 | 4.70E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30834.t1 | MTR_6g465290 | 95.041 | 242 | 11 | 1 | 1 | 241 | 1 | 242 | 9.71e-167 | 460 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30834.t1 | AT5G62140 | 58.768 | 211 | 79 | 3 | 36 | 238 | 27 | 237 | 5.47e-79 | 238 |
Find 38 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGGTTGATCTTGAGTTTA+AGG | 0.089999 | 6.4:-37891735 | MS.gene30834:intron |
| AGCTCTACAACAAAGTTTCT+TGG | 0.240920 | 6.4:-37892804 | MS.gene30834:CDS |
| TGATGGAGTTTCAATTGTTT+TGG | 0.254521 | 6.4:+37893022 | None:intergenic |
| GACCAAGGAAGGAATTTATT+AGG | 0.272245 | 6.4:+37891537 | None:intergenic |
| GGAGGAATAGGATCTGGTTT+TGG | 0.353031 | 6.4:-37892909 | MS.gene30834:CDS |
| TTCTGCTGGTAGCATCTATA+AGG | 0.360928 | 6.4:-37891699 | MS.gene30834:CDS |
| GTGTAAGCCTTATCATTTGA+TGG | 0.365347 | 6.4:+37893005 | None:intergenic |
| CTCTTATGATGAAGAAGAAA+TGG | 0.372803 | 6.4:+37891475 | None:intergenic |
| TTCCTAATAAATTCCTTCCT+TGG | 0.375961 | 6.4:-37891539 | MS.gene30834:CDS |
| GGAAGTATCAATCAAGAATC+TGG | 0.380544 | 6.4:-37892729 | MS.gene30834:CDS |
| TTTATTAGGAAATCATCAAT+AGG | 0.404465 | 6.4:+37891551 | None:intergenic |
| TATGATGCTGAAGGAGGAAT+AGG | 0.412723 | 6.4:-37892921 | MS.gene30834:CDS |
| TCAGCATCATACTCAAAATC+AGG | 0.441137 | 6.4:+37892933 | None:intergenic |
| ATATGAAATTTGCATTGTAT+TGG | 0.443194 | 6.4:+37893122 | None:intergenic |
| AAGTGGAAGAGGCGTGAGAT+TGG | 0.450356 | 6.4:-37891618 | MS.gene30834:CDS |
| TGAAATTTGCATTGTATTGG+TGG | 0.454546 | 6.4:+37893125 | None:intergenic |
| GTTGTAGAGCTTGTTAATGA+TGG | 0.460648 | 6.4:+37892816 | None:intergenic |
| TGAAACTCCATCAAATGATA+AGG | 0.463033 | 6.4:-37893012 | MS.gene30834:CDS |
| AGGCGTGAGATTGGATGAAG+AGG | 0.495524 | 6.4:-37891609 | MS.gene30834:CDS |
| ATACTTCCTTGAAAAGCTTG+TGG | 0.499885 | 6.4:+37892744 | None:intergenic |
| ACCAGCAGAAAACAAGAACT+TGG | 0.514821 | 6.4:+37891712 | None:intergenic |
| CAACACTGTCTTCACAAGTA+GGG | 0.517386 | 6.4:+37891670 | None:intergenic |
| GCTGAAGGAGGAATAGGATC+TGG | 0.519400 | 6.4:-37892915 | MS.gene30834:CDS |
| GGCGTGAGATTGGATGAAGA+GGG | 0.528940 | 6.4:-37891608 | MS.gene30834:CDS |
| GTTGACATCTGAAGAATCAA+AGG | 0.532463 | 6.4:-37891651 | MS.gene30834:CDS |
| TCAACACTGTCTTCACAAGT+AGG | 0.539949 | 6.4:+37891669 | None:intergenic |
| TCAAAGGGAACATTGAAAAG+TGG | 0.561855 | 6.4:-37891635 | MS.gene30834:CDS |
| TTGACATCTGAAGAATCAAA+GGG | 0.565533 | 6.4:-37891650 | MS.gene30834:CDS |
| CTCAGTAGGTAGACCAAGGA+AGG | 0.566579 | 6.4:+37891526 | None:intergenic |
| GGAACATTGAAAAGTGGAAG+AGG | 0.579567 | 6.4:-37891629 | MS.gene30834:CDS |
| TGAAGAGGGAAAGTGCAGAC+TGG | 0.610863 | 6.4:-37891594 | MS.gene30834:CDS |
| TTTGAGTATGATGCTGAAGG+AGG | 0.619744 | 6.4:-37892927 | MS.gene30834:CDS |
| AACACTGTCTTCACAAGTAG+GGG | 0.620170 | 6.4:+37891671 | None:intergenic |
| GAGGGAAAGTGCAGACTGGT+TGG | 0.627517 | 6.4:-37891590 | MS.gene30834:CDS |
| AGTTCTGCAAGACACTCAGT+AGG | 0.631737 | 6.4:+37891512 | None:intergenic |
| GACACTCAGTAGGTAGACCA+AGG | 0.644039 | 6.4:+37891522 | None:intergenic |
| ACTGTCTTCACAAGTAGGGG+TGG | 0.644274 | 6.4:+37891674 | None:intergenic |
| TATCAATCAAGAATCTGGCA+AGG | 0.648241 | 6.4:-37892724 | MS.gene30834:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTATTTTTTTCTTACA+AGG | - | chr6.4:37892877-37892896 | MS.gene30834:CDS | 10.0% |
| !!! | CTATTTTAATATTATATACT+AGG | + | chr6.4:37892504-37892523 | None:intergenic | 10.0% |
| !!! | AACATTTTCCAAAAAATTTA+AGG | + | chr6.4:37892036-37892055 | None:intergenic | 15.0% |
| !!! | ACATTTTCCAAAAAATTTAA+GGG | + | chr6.4:37892035-37892054 | None:intergenic | 15.0% |
| !!! | TTATTTTCTCAAAATTTTAC+TGG | - | chr6.4:37892785-37892804 | MS.gene30834:CDS | 15.0% |
| !! | ACAATATCAATCTTCAAAAA+TGG | + | chr6.4:37891865-37891884 | None:intergenic | 20.0% |
| !! | ATATGAAATTTGCATTGTAT+TGG | + | chr6.4:37891511-37891530 | None:intergenic | 20.0% |
| !! | CAAATATAGTAAGAATTTCT+TGG | - | chr6.4:37892088-37892107 | MS.gene30834:intron | 20.0% |
| !! | TACAATCTTTATAGCTTATA+TGG | + | chr6.4:37891936-37891955 | None:intergenic | 20.0% |
| !! | TGGTGGTTATTAAAAAATTT+GGG | + | chr6.4:37892220-37892239 | None:intergenic | 20.0% |
| !! | TTCAATATAATCACATATGT+CGG | + | chr6.4:37892760-37892779 | None:intergenic | 20.0% |
| !! | TTTATTAGGAAATCATCAAT+AGG | + | chr6.4:37893082-37893101 | None:intergenic | 20.0% |
| !!! | TATGTATCCCTTAAATTTTT+TGG | - | chr6.4:37892025-37892044 | MS.gene30834:intron | 20.0% |
| !!! | TTATTTTGTTTTTCACTTGA+GGG | + | chr6.4:37891580-37891599 | None:intergenic | 20.0% |
| !!! | TTGGTGGTTATTAAAAAATT+TGG | + | chr6.4:37892221-37892240 | None:intergenic | 20.0% |
| !!! | TTTATTTTGTTTTTCACTTG+AGG | + | chr6.4:37891581-37891600 | None:intergenic | 20.0% |
| ! | AAAATGTTAACTAGTACTCT+AGG | - | chr6.4:37892048-37892067 | MS.gene30834:intron | 25.0% |
| ! | AAATGTTAACTAGTACTCTA+GGG | - | chr6.4:37892049-37892068 | MS.gene30834:intron | 25.0% |
| ! | ACAAACTCTTATCATTAAAC+TGG | + | chr6.4:37892328-37892347 | None:intergenic | 25.0% |
| ! | ACTATATTTGTTTCCTTAAC+TGG | + | chr6.4:37892078-37892097 | None:intergenic | 25.0% |
| ! | ATATCAATCTTCAAAAATGG+TGG | + | chr6.4:37891862-37891881 | None:intergenic | 25.0% |
| ! | CTTGAAGGAATAAACATTTA+GGG | - | chr6.4:37892626-37892645 | MS.gene30834:intron | 25.0% |
| ! | TCTTGAAGGAATAAACATTT+AGG | - | chr6.4:37892625-37892644 | MS.gene30834:intron | 25.0% |
| ! | TTTCTTTAATCAATACTCGA+AGG | - | chr6.4:37892178-37892197 | MS.gene30834:intron | 25.0% |
| ! | TTTGTTAACTAACTGACTAA+CGG | - | chr6.4:37892548-37892567 | MS.gene30834:intron | 25.0% |
| !! | AACAGAAGTTGTAAGTTTTA+CGG | + | chr6.4:37892160-37892179 | None:intergenic | 25.0% |
| AAACCATTAACTATGAAGCA+CGG | + | chr6.4:37892853-37892872 | None:intergenic | 30.0% | |
| AATGCAACCATATCTCAATT+TGG | + | chr6.4:37892240-37892259 | None:intergenic | 30.0% | |
| ATAACCACCAAATTGAGATA+TGG | - | chr6.4:37892230-37892249 | MS.gene30834:intron | 30.0% | |
| GATCCTGTAATATTAGAAGA+GGG | - | chr6.4:37892402-37892421 | MS.gene30834:intron | 30.0% | |
| TACCATTGATACCAAAAACT+TGG | + | chr6.4:37892375-37892394 | None:intergenic | 30.0% | |
| TGAAACTCCATCAAATGATA+AGG | - | chr6.4:37891618-37891637 | MS.gene30834:CDS | 30.0% | |
| TGAAATTTGCATTGTATTGG+TGG | + | chr6.4:37891508-37891527 | None:intergenic | 30.0% | |
| TGATCCTGTAATATTAGAAG+AGG | - | chr6.4:37892401-37892420 | MS.gene30834:intron | 30.0% | |
| TTCCTAATAAATTCCTTCCT+TGG | - | chr6.4:37893091-37893110 | MS.gene30834:CDS | 30.0% | |
| TTGACATCTGAAGAATCAAA+GGG | - | chr6.4:37892980-37892999 | MS.gene30834:CDS | 30.0% | |
| TTTAAGGGATACATATAGCT+AGG | + | chr6.4:37892020-37892039 | None:intergenic | 30.0% | |
| ! | TATGTGTCTACACCTTTTAA+TGG | - | chr6.4:37891976-37891995 | MS.gene30834:intron | 30.0% |
| ! | TGATGGAGTTTCAATTGTTT+TGG | + | chr6.4:37891611-37891630 | None:intergenic | 30.0% |
| ACTCCCTCTTCTAATATTAC+AGG | + | chr6.4:37892408-37892427 | None:intergenic | 35.0% | |
| AGCTCTACAACAAAGTTTCT+TGG | - | chr6.4:37891826-37891845 | MS.gene30834:intron | 35.0% | |
| AGTCTAAATCCTCGAGTTAT+TGG | - | chr6.4:37892425-37892444 | MS.gene30834:intron | 35.0% | |
| ATACTTCCTTGAAAAGCTTG+TGG | + | chr6.4:37891889-37891908 | None:intergenic | 35.0% | |
| ATTTCACAACCAATAACTCG+AGG | + | chr6.4:37892437-37892456 | None:intergenic | 35.0% | |
| CAACATAGTCCAGTAACAAT+TGG | + | chr6.4:37892680-37892699 | None:intergenic | 35.0% | |
| GACCAAGGAAGGAATTTATT+AGG | + | chr6.4:37893096-37893115 | None:intergenic | 35.0% | |
| GAGTCAAATCCAATTGTTAC+TGG | - | chr6.4:37892668-37892687 | MS.gene30834:intron | 35.0% | |
| GTTGACATCTGAAGAATCAA+AGG | - | chr6.4:37892979-37892998 | MS.gene30834:CDS | 35.0% | |
| TATCAATCAAGAATCTGGCA+AGG | - | chr6.4:37891906-37891925 | MS.gene30834:intron | 35.0% | |
| TCAAAGGGAACATTGAAAAG+TGG | - | chr6.4:37892995-37893014 | MS.gene30834:CDS | 35.0% | |
| TCAGCATCATACTCAAAATC+AGG | + | chr6.4:37891700-37891719 | None:intergenic | 35.0% | |
| TGACTAAACTAACGAACGAA+AGG | - | chr6.4:37892584-37892603 | MS.gene30834:intron | 35.0% | |
| TTTGAAGACTGAACTCTTGA+AGG | - | chr6.4:37892611-37892630 | MS.gene30834:intron | 35.0% | |
| ! | AAAGACCAAGTGCCATTAAA+AGG | + | chr6.4:37891991-37892010 | None:intergenic | 35.0% |
| ! | AGAGAAAGAGTCCAAGTTTT+TGG | - | chr6.4:37892361-37892380 | MS.gene30834:intron | 35.0% |
| ! | ATTGTTCCACAAGCTTTTCA+AGG | - | chr6.4:37891880-37891899 | MS.gene30834:intron | 35.0% |
| ! | CAAGGTTGATCTTGAGTTTA+AGG | - | chr6.4:37892895-37892914 | MS.gene30834:CDS | 35.0% |
| ! | CTCTAATTGATAGTATGTCC+TGG | - | chr6.4:37892709-37892728 | MS.gene30834:intron | 35.0% |
| ! | GTGTAAGCCTTATCATTTGA+TGG | + | chr6.4:37891628-37891647 | None:intergenic | 35.0% |
| !! | GATTTTGAGTATGATGCTGA+AGG | - | chr6.4:37891700-37891719 | MS.gene30834:CDS | 35.0% |
| !! | GGAAGTATCAATCAAGAATC+TGG | - | chr6.4:37891901-37891920 | MS.gene30834:intron | 35.0% |
| !! | GTTGTAGAGCTTGTTAATGA+TGG | + | chr6.4:37891817-37891836 | None:intergenic | 35.0% |
| !!! | GTCCAAGTTTTTGGTATCAA+TGG | - | chr6.4:37892370-37892389 | MS.gene30834:intron | 35.0% |
| AACACTGTCTTCACAAGTAG+GGG | + | chr6.4:37892962-37892981 | None:intergenic | 40.0% | |
| ACCAGCAGAAAACAAGAACT+TGG | + | chr6.4:37892921-37892940 | None:intergenic | 40.0% | |
| CAACACTGTCTTCACAAGTA+GGG | + | chr6.4:37892963-37892982 | None:intergenic | 40.0% | |
| GCAACCATATCTCAATTTGG+TGG | + | chr6.4:37892237-37892256 | None:intergenic | 40.0% | |
| GGAACATTGAAAAGTGGAAG+AGG | - | chr6.4:37893001-37893020 | MS.gene30834:CDS | 40.0% | |
| TATGATGCTGAAGGAGGAAT+AGG | - | chr6.4:37891709-37891728 | MS.gene30834:CDS | 40.0% | |
| TCAACACTGTCTTCACAAGT+AGG | + | chr6.4:37892964-37892983 | None:intergenic | 40.0% | |
| TGTCCGTGCTTCATAGTTAA+TGG | - | chr6.4:37892847-37892866 | MS.gene30834:CDS | 40.0% | |
| TTCTGCTGGTAGCATCTATA+AGG | - | chr6.4:37892931-37892950 | MS.gene30834:CDS | 40.0% | |
| TTTGAGTATGATGCTGAAGG+AGG | - | chr6.4:37891703-37891722 | MS.gene30834:CDS | 40.0% | |
| ! | CTACACCTTTTAATGGCACT+TGG | - | chr6.4:37891983-37892002 | MS.gene30834:intron | 40.0% |
| !! | ACAAAAAGTGCTTGCAAGCT+TGG | - | chr6.4:37891661-37891680 | MS.gene30834:CDS | 40.0% |
| !! | TACTCTAGGGTTACCAGTTA+AGG | - | chr6.4:37892062-37892081 | MS.gene30834:intron | 40.0% |
| !!! | AGGATCTGGTTTTGGTTCAA+AGG | - | chr6.4:37891729-37891748 | MS.gene30834:CDS | 40.0% |
| AGTTCTGCAAGACACTCAGT+AGG | + | chr6.4:37893121-37893140 | None:intergenic | 45.0% | |
| !! | GGAGGAATAGGATCTGGTTT+TGG | - | chr6.4:37891721-37891740 | MS.gene30834:CDS | 45.0% |
| !!! | GCCAAGTTCTTGTTTTCTGC+TGG | - | chr6.4:37892917-37892936 | MS.gene30834:CDS | 45.0% |
| ACCGGAACACGACACTGATA+CGG | + | chr6.4:37892822-37892841 | None:intergenic | 50.0% | |
| ACTGTCTTCACAAGTAGGGG+TGG | + | chr6.4:37892959-37892978 | None:intergenic | 50.0% | |
| AGGCGTGAGATTGGATGAAG+AGG | - | chr6.4:37893021-37893040 | MS.gene30834:CDS | 50.0% | |
| CTCAGTAGGTAGACCAAGGA+AGG | + | chr6.4:37893107-37893126 | None:intergenic | 50.0% | |
| GACACTCAGTAGGTAGACCA+AGG | + | chr6.4:37893111-37893130 | None:intergenic | 50.0% | |
| GCTGAAGGAGGAATAGGATC+TGG | - | chr6.4:37891715-37891734 | MS.gene30834:CDS | 50.0% | |
| GGCGTGAGATTGGATGAAGA+GGG | - | chr6.4:37893022-37893041 | MS.gene30834:CDS | 50.0% | |
| GTGTCTGACATGTGTCGTGT+TGG | - | chr6.4:37892731-37892750 | MS.gene30834:CDS | 50.0% | |
| TCCGTATCAGTGTCGTGTTC+CGG | - | chr6.4:37892818-37892837 | MS.gene30834:CDS | 50.0% | |
| ! | AAGTGGAAGAGGCGTGAGAT+TGG | - | chr6.4:37893012-37893031 | MS.gene30834:CDS | 50.0% |
| ! | TGAAGAGGGAAAGTGCAGAC+TGG | - | chr6.4:37893036-37893055 | MS.gene30834:CDS | 50.0% |
| CACGACACATGTCAGACACC+AGG | + | chr6.4:37892730-37892749 | None:intergenic | 55.0% | |
| TGAAGCACGGACACAGACAC+CGG | + | chr6.4:37892840-37892859 | None:intergenic | 55.0% | |
| ! | GAGGGAAAGTGCAGACTGGT+TGG | - | chr6.4:37893040-37893059 | MS.gene30834:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 37891478 | 37893174 | 37891478 | ID=MS.gene30834 |
| chr6.4 | mRNA | 37891478 | 37893174 | 37891478 | ID=MS.gene30834.t1;Parent=MS.gene30834 |
| chr6.4 | exon | 37892725 | 37893174 | 37892725 | ID=MS.gene30834.t1.exon1;Parent=MS.gene30834.t1 |
| chr6.4 | CDS | 37892725 | 37893174 | 37892725 | ID=cds.MS.gene30834.t1;Parent=MS.gene30834.t1 |
| chr6.4 | exon | 37891478 | 37891753 | 37891478 | ID=MS.gene30834.t1.exon2;Parent=MS.gene30834.t1 |
| chr6.4 | CDS | 37891478 | 37891753 | 37891478 | ID=cds.MS.gene30834.t1;Parent=MS.gene30834.t1 |
| Gene Sequence |
| Protein sequence |
