Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39909.t1 | XP_013468578.1 | 96.4 | 137 | 5 | 0 | 1 | 137 | 1 | 137 | 1.10E-62 | 249.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39909.t1 | Q41387 | 59.1 | 137 | 56 | 0 | 1 | 137 | 1 | 137 | 1.6e-33 | 143.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39909.t1 | A0A072VWZ4 | 96.4 | 137 | 5 | 0 | 1 | 137 | 1 | 137 | 7.9e-63 | 249.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene39909 | 0.814249 | 1.70E-51 | -1.69E-46 |
| MS.gene049173 | MS.gene39909 | 0.817319 | 3.51E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene39909 | 0.818516 | 1.88E-52 | -1.69E-46 |
| MS.gene049222 | MS.gene39909 | 0.849693 | 2.75E-60 | -1.69E-46 |
| MS.gene049285 | MS.gene39909 | 0.839143 | 1.89E-57 | -1.69E-46 |
| MS.gene049320 | MS.gene39909 | 0.871402 | 7.02E-67 | -1.69E-46 |
| MS.gene049427 | MS.gene39909 | 0.844962 | 5.46E-59 | -1.69E-46 |
| MS.gene049609 | MS.gene39909 | 0.88583 | 5.80E-72 | -1.69E-46 |
| MS.gene049615 | MS.gene39909 | 0.800923 | 1.15E-48 | -1.69E-46 |
| MS.gene049633 | MS.gene39909 | 0.801862 | 7.37E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene39909 | 0.810118 | 1.35E-50 | -1.69E-46 |
| MS.gene049662 | MS.gene39909 | 0.81245 | 4.21E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene39909 | 0.812149 | 4.90E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene39909 | 0.816195 | 6.27E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene39909 | 0.838239 | 3.24E-57 | -1.69E-46 |
| MS.gene050165 | MS.gene39909 | 0.824233 | 9.02E-54 | -1.69E-46 |
| MS.gene050333 | MS.gene39909 | 0.809036 | 2.31E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene39909 | 0.869032 | 4.20E-66 | -1.69E-46 |
| MS.gene050504 | MS.gene39909 | 0.808523 | 2.97E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene39909 | 0.836493 | 9.06E-57 | -1.69E-46 |
| MS.gene050538 | MS.gene39909 | 0.871314 | 7.51E-67 | -1.69E-46 |
| MS.gene050593 | MS.gene39909 | 0.871431 | 6.87E-67 | -1.69E-46 |
| MS.gene050612 | MS.gene39909 | 0.827378 | 1.62E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene39909 | 0.826605 | 2.47E-54 | -1.69E-46 |
| MS.gene050675 | MS.gene39909 | 0.841706 | 4.04E-58 | -1.69E-46 |
| MS.gene050699 | MS.gene39909 | 0.845321 | 4.37E-59 | -1.69E-46 |
| MS.gene050747 | MS.gene39909 | 0.837166 | 6.10E-57 | -1.69E-46 |
| MS.gene050767 | MS.gene39909 | 0.846997 | 1.53E-59 | -1.69E-46 |
| MS.gene050770 | MS.gene39909 | 0.850052 | 2.18E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene39909 | 0.898121 | 7.23E-77 | -1.69E-46 |
| MS.gene050783 | MS.gene39909 | 0.803482 | 3.41E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene39909 | 0.837913 | 3.93E-57 | -1.69E-46 |
| MS.gene050950 | MS.gene39909 | 0.843787 | 1.13E-58 | -1.69E-46 |
| MS.gene050986 | MS.gene39909 | 0.832404 | 9.64E-56 | -1.69E-46 |
| MS.gene05100 | MS.gene39909 | 0.815456 | 9.16E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene39909 | 0.899505 | 1.86E-77 | -1.69E-46 |
| MS.gene051228 | MS.gene39909 | 0.847918 | 8.53E-60 | -1.69E-46 |
| MS.gene051387 | MS.gene39909 | 0.824698 | 7.01E-54 | -1.69E-46 |
| MS.gene051434 | MS.gene39909 | 0.800283 | 1.55E-48 | -1.69E-46 |
| MS.gene051551 | MS.gene39909 | 0.807078 | 6.03E-50 | -1.69E-46 |
| MS.gene051617 | MS.gene39909 | 0.840655 | 7.63E-58 | -1.69E-46 |
| MS.gene051666 | MS.gene39909 | 0.836311 | 1.01E-56 | -1.69E-46 |
| MS.gene051810 | MS.gene39909 | 0.878846 | 2.02E-69 | -1.69E-46 |
| MS.gene051858 | MS.gene39909 | 0.827321 | 1.67E-54 | -1.69E-46 |
| MS.gene052271 | MS.gene39909 | 0.848015 | 8.02E-60 | -1.69E-46 |
| MS.gene052290 | MS.gene39909 | 0.800892 | 1.16E-48 | -1.69E-46 |
| MS.gene052310 | MS.gene39909 | 0.814118 | 1.81E-51 | -1.69E-46 |
| MS.gene052379 | MS.gene39909 | 0.829259 | 5.69E-55 | -1.69E-46 |
| MS.gene052421 | MS.gene39909 | 0.809408 | 1.92E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene39909 | 0.807216 | 5.63E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene39909 | 0.840248 | 9.75E-58 | -1.69E-46 |
| MS.gene052508 | MS.gene39909 | 0.802795 | 4.74E-49 | -1.69E-46 |
| MS.gene052510 | MS.gene39909 | 0.803497 | 3.39E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene39909 | 0.826233 | 3.04E-54 | -1.69E-46 |
| MS.gene052670 | MS.gene39909 | 0.801389 | 9.21E-49 | -1.69E-46 |
| MS.gene053009 | MS.gene39909 | 0.844113 | 9.24E-59 | -1.69E-46 |
| MS.gene053103 | MS.gene39909 | 0.81216 | 4.88E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene39909 | 0.87652 | 1.31E-68 | -1.69E-46 |
| MS.gene053107 | MS.gene39909 | 0.854228 | 1.42E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene39909 | 0.870977 | 9.70E-67 | -1.69E-46 |
| MS.gene053209 | MS.gene39909 | 0.852162 | 5.53E-61 | -1.69E-46 |
| MS.gene053213 | MS.gene39909 | 0.883811 | 3.27E-71 | -1.69E-46 |
| MS.gene053286 | MS.gene39909 | 0.874656 | 5.70E-68 | -1.69E-46 |
| MS.gene053322 | MS.gene39909 | 0.850196 | 1.99E-60 | -1.69E-46 |
| MS.gene053436 | MS.gene39909 | 0.803298 | 3.73E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene39909 | 0.889946 | 1.54E-73 | -1.69E-46 |
| MS.gene053632 | MS.gene39909 | 0.829981 | 3.80E-55 | -1.69E-46 |
| MS.gene053916 | MS.gene39909 | 0.817214 | 3.70E-52 | -1.69E-46 |
| MS.gene054376 | MS.gene39909 | 0.851246 | 1.01E-60 | -1.69E-46 |
| MS.gene054600 | MS.gene39909 | 0.857193 | 1.93E-62 | -1.69E-46 |
| MS.gene054642 | MS.gene39909 | 0.878595 | 2.47E-69 | -1.69E-46 |
| MS.gene054802 | MS.gene39909 | 0.810035 | 1.41E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene39909 | 0.808471 | 3.05E-50 | -1.69E-46 |
| MS.gene054804 | MS.gene39909 | 0.811882 | 5.61E-51 | -1.69E-46 |
| MS.gene054927 | MS.gene39909 | 0.845789 | 3.26E-59 | -1.69E-46 |
| MS.gene054954 | MS.gene39909 | 0.802143 | 6.45E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene39909 | 0.839081 | 1.96E-57 | -1.69E-46 |
| MS.gene055116 | MS.gene39909 | 0.858136 | 1.01E-62 | -1.69E-46 |
| MS.gene055206 | MS.gene39909 | 0.830238 | 3.29E-55 | -1.69E-46 |
| MS.gene055212 | MS.gene39909 | 0.836862 | 7.29E-57 | -1.69E-46 |
| MS.gene055224 | MS.gene39909 | 0.802139 | 6.46E-49 | -1.69E-46 |
| MS.gene055254 | MS.gene39909 | 0.807707 | 4.43E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene39909 | 0.846703 | 1.84E-59 | -1.69E-46 |
| MS.gene055362 | MS.gene39909 | 0.847918 | 8.53E-60 | -1.69E-46 |
| MS.gene055397 | MS.gene39909 | 0.8388 | 2.32E-57 | -1.69E-46 |
| MS.gene055499 | MS.gene39909 | 0.860499 | 1.98E-63 | -1.69E-46 |
| MS.gene055502 | MS.gene39909 | 0.830919 | 2.24E-55 | -1.69E-46 |
| MS.gene055577 | MS.gene39909 | 0.833964 | 3.94E-56 | -1.69E-46 |
| MS.gene055647 | MS.gene39909 | 0.852743 | 3.78E-61 | -1.69E-46 |
| MS.gene055722 | MS.gene39909 | 0.882037 | 1.46E-70 | -1.69E-46 |
| MS.gene05592 | MS.gene39909 | 0.882413 | 1.06E-70 | -1.69E-46 |
| MS.gene055941 | MS.gene39909 | 0.811233 | 7.76E-51 | -1.69E-46 |
| MS.gene05602 | MS.gene39909 | 0.821959 | 3.06E-53 | -1.69E-46 |
| MS.gene056083 | MS.gene39909 | 0.827219 | 1.77E-54 | -1.69E-46 |
| MS.gene056191 | MS.gene39909 | 0.801984 | 6.96E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene39909 | 0.836702 | 8.02E-57 | -1.69E-46 |
| MS.gene056407 | MS.gene39909 | 0.817205 | 3.72E-52 | -1.69E-46 |
| MS.gene056410 | MS.gene39909 | 0.812849 | 3.45E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene39909 | 0.815909 | 7.26E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene39909 | 0.826505 | 2.61E-54 | -1.69E-46 |
| MS.gene056609 | MS.gene39909 | 0.81511 | 1.09E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene39909 | 0.864402 | 1.25E-64 | -1.69E-46 |
| MS.gene056897 | MS.gene39909 | 0.824017 | 1.01E-53 | -1.69E-46 |
| MS.gene056947 | MS.gene39909 | 0.852981 | 3.23E-61 | -1.69E-46 |
| MS.gene057011 | MS.gene39909 | 0.829916 | 3.94E-55 | -1.69E-46 |
| MS.gene057076 | MS.gene39909 | 0.858376 | 8.60E-63 | -1.69E-46 |
| MS.gene057124 | MS.gene39909 | 0.80654 | 7.83E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene39909 | 0.870935 | 1.00E-66 | -1.69E-46 |
| MS.gene057146 | MS.gene39909 | 0.814973 | 1.17E-51 | -1.69E-46 |
| MS.gene057196 | MS.gene39909 | 0.801271 | 9.74E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene39909 | 0.914665 | 1.47E-84 | -1.69E-46 |
| MS.gene05735 | MS.gene39909 | 0.806247 | 9.03E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene39909 | 0.863362 | 2.63E-64 | -1.69E-46 |
| MS.gene057658 | MS.gene39909 | 0.801385 | 9.23E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene39909 | 0.80218 | 6.34E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene39909 | 0.849472 | 3.16E-60 | -1.69E-46 |
| MS.gene058082 | MS.gene39909 | 0.818178 | 2.24E-52 | -1.69E-46 |
| MS.gene058130 | MS.gene39909 | 0.845833 | 3.17E-59 | -1.69E-46 |
| MS.gene058233 | MS.gene39909 | 0.807535 | 4.82E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene39909 | 0.832076 | 1.16E-55 | -1.69E-46 |
| MS.gene058323 | MS.gene39909 | 0.839591 | 1.45E-57 | -1.69E-46 |
| MS.gene05837 | MS.gene39909 | 0.828979 | 6.65E-55 | -1.69E-46 |
| MS.gene058419 | MS.gene39909 | 0.86626 | 3.25E-65 | -1.69E-46 |
| MS.gene058544 | MS.gene39909 | 0.827953 | 1.18E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene39909 | 0.816908 | 4.34E-52 | -1.69E-46 |
| MS.gene058723 | MS.gene39909 | 0.886761 | 2.58E-72 | -1.69E-46 |
| MS.gene058748 | MS.gene39909 | 0.83759 | 4.75E-57 | -1.69E-46 |
| MS.gene058773 | MS.gene39909 | 0.839706 | 1.35E-57 | -1.69E-46 |
| MS.gene05883 | MS.gene39909 | 0.813442 | 2.55E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene39909 | 0.822264 | 2.60E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene39909 | 0.816808 | 4.57E-52 | -1.69E-46 |
| MS.gene059029 | MS.gene39909 | 0.860496 | 1.98E-63 | -1.69E-46 |
| MS.gene05913 | MS.gene39909 | 0.836634 | 8.34E-57 | -1.69E-46 |
| MS.gene05934 | MS.gene39909 | 0.838938 | 2.14E-57 | -1.69E-46 |
| MS.gene059536 | MS.gene39909 | 0.845821 | 3.20E-59 | -1.69E-46 |
| MS.gene059676 | MS.gene39909 | 0.803092 | 4.11E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene39909 | 0.846632 | 1.92E-59 | -1.69E-46 |
| MS.gene059688 | MS.gene39909 | 0.861179 | 1.23E-63 | -1.69E-46 |
| MS.gene059692 | MS.gene39909 | 0.863054 | 3.27E-64 | -1.69E-46 |
| MS.gene059695 | MS.gene39909 | 0.867478 | 1.33E-65 | -1.69E-46 |
| MS.gene059711 | MS.gene39909 | 0.801561 | 8.50E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene39909 | 0.851687 | 7.55E-61 | -1.69E-46 |
| MS.gene059858 | MS.gene39909 | 0.812891 | 3.37E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene39909 | 0.81199 | 5.31E-51 | -1.69E-46 |
| MS.gene059902 | MS.gene39909 | 0.829463 | 5.08E-55 | -1.69E-46 |
| MS.gene059960 | MS.gene39909 | 0.869004 | 4.29E-66 | -1.69E-46 |
| MS.gene060193 | MS.gene39909 | 0.80241 | 5.69E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene39909 | 0.888125 | 7.79E-73 | -1.69E-46 |
| MS.gene060818 | MS.gene39909 | 0.808872 | 2.50E-50 | -1.69E-46 |
| MS.gene060835 | MS.gene39909 | 0.870014 | 2.01E-66 | -1.69E-46 |
| MS.gene060837 | MS.gene39909 | 0.818336 | 2.07E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene39909 | 0.865709 | 4.85E-65 | -1.69E-46 |
| MS.gene061041 | MS.gene39909 | 0.842723 | 2.17E-58 | -1.69E-46 |
| MS.gene061051 | MS.gene39909 | -0.814082 | 1.85E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene39909 | 0.820802 | 5.66E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene39909 | 0.804838 | 1.78E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene39909 | 0.855163 | 7.59E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39909.t1 | MTR_1g070495 | 96.350 | 137 | 5 | 0 | 1 | 137 | 1 | 137 | 5.68e-93 | 265 |
| MS.gene39909.t1 | MTR_7g096150 | 76.642 | 137 | 31 | 1 | 1 | 137 | 1 | 136 | 1.12e-66 | 198 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39909.t1 | AT2G30570 | 63.043 | 138 | 45 | 3 | 1 | 137 | 1 | 133 | 2.30e-46 | 147 |
Find 42 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGTGTGTTCGGTCTTATC+TGG | 0.197833 | 1.4:+57899893 | MS.gene39909:CDS |
| GTTTCATCCTCTCCTGTTCT+TGG | 0.207350 | 1.4:+57898989 | MS.gene39909:CDS |
| TCCACCAATGCCATGGCTGC+TGG | 0.247914 | 1.4:-57899799 | None:intergenic |
| GGACTCAGCAACAACCTTCT+TGG | 0.302937 | 1.4:+57899859 | MS.gene39909:CDS |
| CTTCCCACCTTAGCCATTGT+TGG | 0.376821 | 1.4:-57899667 | None:intergenic |
| TTCATTTCAAGCAATTTAAA+GGG | 0.388432 | 1.4:-57899971 | None:intergenic |
| TAAGACCGAACACACCAAAA+AGG | 0.399989 | 1.4:-57899888 | None:intergenic |
| CCACCAATGCCATGGCTGCT+GGG | 0.401839 | 1.4:-57899798 | None:intergenic |
| CCCAGCAGCCATGGCATTGG+TGG | 0.403670 | 1.4:+57899798 | MS.gene39909:CDS |
| GAAGGAACCGGGCTTCCCTT+TGG | 0.425729 | 1.4:+57899838 | MS.gene39909:CDS |
| CTTGAAGAGGATGAAGAGTC+TGG | 0.434178 | 1.4:+57899943 | MS.gene39909:CDS |
| AGAATGAGCACTGAAGGAAC+CGG | 0.435090 | 1.4:+57899826 | MS.gene39909:CDS |
| TGGTGTGTTCGGTCTTATCT+GGG | 0.435857 | 1.4:+57899894 | MS.gene39909:CDS |
| AAGAACAACATACCAAGAAC+AGG | 0.443082 | 1.4:-57899001 | None:intergenic |
| GGGAAGAGTGAGGTGTTCAA+TGG | 0.445647 | 1.4:+57899684 | MS.gene39909:CDS |
| AGCAACTCAAACATTGGAAT+GGG | 0.465387 | 1.4:+57899733 | MS.gene39909:CDS |
| AATGAAAGCAACTCAAACAT+TGG | 0.469451 | 1.4:+57899727 | MS.gene39909:CDS |
| TCAGCAACAACCTTCTTGGT+TGG | 0.473672 | 1.4:+57899863 | MS.gene39909:CDS |
| AAGAGTGAGGTGTTCAATGG+AGG | 0.507963 | 1.4:+57899687 | MS.gene39909:CDS |
| AAGCAACTCAAACATTGGAA+TGG | 0.523456 | 1.4:+57899732 | MS.gene39909:CDS |
| AAGCCCAGCAGCCATGGCAT+TGG | 0.535185 | 1.4:+57899795 | MS.gene39909:CDS |
| ATTGCCAACAATGGCTAAGG+TGG | 0.548615 | 1.4:+57899663 | MS.gene39909:CDS |
| AGAGTGAGGTGTTCAATGGA+GGG | 0.548761 | 1.4:+57899688 | MS.gene39909:CDS |
| TTGCTTTCATTCACAGAAGG+TGG | 0.553252 | 1.4:-57899715 | None:intergenic |
| AAGGTTGTTGCTGAGTCCAA+AGG | 0.556907 | 1.4:-57899854 | None:intergenic |
| TCTTTCATCCACCAATGCCA+TGG | 0.558482 | 1.4:-57899806 | None:intergenic |
| GCTGAGTCCAAAGGGAAGCC+CGG | 0.571088 | 1.4:-57899845 | None:intergenic |
| AGGATTGCCAACAATGGCTA+AGG | 0.571754 | 1.4:+57899660 | MS.gene39909:intron |
| GAGTTGCTTTCATTCACAGA+AGG | 0.574697 | 1.4:-57899718 | None:intergenic |
| AGGTTGTTGCTGAGTCCAAA+GGG | 0.582023 | 1.4:-57899853 | None:intergenic |
| GATGAAAGAATGAGCACTGA+AGG | 0.583467 | 1.4:+57899820 | MS.gene39909:CDS |
| GAATGAGCACTGAAGGAACC+GGG | 0.588005 | 1.4:+57899827 | MS.gene39909:CDS |
| CTACGCCTCGAGTCTTGAAG+AGG | 0.588236 | 1.4:+57899930 | MS.gene39909:CDS |
| TATGTCAAGCCCAGCAGCCA+TGG | 0.588442 | 1.4:+57899789 | MS.gene39909:CDS |
| AGTTGTAGTGGTAGCTGAGA+TGG | 0.594313 | 1.4:-57898921 | None:intergenic |
| TTATTCAGGATTGCCAACAA+TGG | 0.611540 | 1.4:+57899654 | MS.gene39909:intron |
| TTCATCCTCTTCAAGACTCG+AGG | 0.612569 | 1.4:-57899935 | None:intergenic |
| GGTGATCGATGCAGTTGTAG+TGG | 0.624319 | 1.4:-57898933 | None:intergenic |
| TTGCCAACAATGGCTAAGGT+GGG | 0.634166 | 1.4:+57899664 | MS.gene39909:CDS |
| CAACATACCAAGAACAGGAG+AGG | 0.652090 | 1.4:-57898996 | None:intergenic |
| TGGCTAAGGTGGGAAGAGTG+AGG | 0.666585 | 1.4:+57899674 | MS.gene39909:CDS |
| GGAGAGGATGAAACTGCAAG+AGG | 0.693611 | 1.4:-57898980 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATGAAAAATCAATTAAAAA+TGG | - | chr1.4:57899595-57899614 | None:intergenic | 15.0% |
| !!! | AACATTCAATTATTTTATTC+AGG | + | chr1.4:57899640-57899659 | MS.gene39909:intron | 15.0% |
| !!! | ATTTTTAGATTTTTTTTTCC+CGG | + | chr1.4:57899353-57899372 | MS.gene39909:intron | 15.0% |
| !! | TAACTAATGCATTCTATATT+GGG | - | chr1.4:57899166-57899185 | None:intergenic | 20.0% |
| !! | TATCATGCTATTCAATATTT+AGG | - | chr1.4:57899040-57899059 | None:intergenic | 20.0% |
| !! | TTGATATTTGATATTACGAT+AGG | + | chr1.4:57899194-57899213 | MS.gene39909:intron | 20.0% |
| !!! | AATATTTAGGATGAAAATCA+AGG | - | chr1.4:57899027-57899046 | None:intergenic | 20.0% |
| !!! | AGATATATATGTTTTAAGAG+TGG | + | chr1.4:57899138-57899157 | MS.gene39909:intron | 20.0% |
| !!! | TTTTTAGATTTTTTTTTCCC+GGG | + | chr1.4:57899354-57899373 | MS.gene39909:intron | 20.0% |
| ! | ATCATTACAAAATCAAGAGA+CGG | - | chr1.4:57899325-57899344 | None:intergenic | 25.0% |
| ! | CTAACTAATGCATTCTATAT+TGG | - | chr1.4:57899167-57899186 | None:intergenic | 25.0% |
| !!! | GAATTTTATTTGTACTTCTG+TGG | + | chr1.4:57899111-57899130 | MS.gene39909:intron | 25.0% |
| AACCAAACAGTAGTATAGAA+TGG | - | chr1.4:57899435-57899454 | None:intergenic | 30.0% | |
| AATGAAAGCAACTCAAACAT+TGG | + | chr1.4:57899727-57899746 | MS.gene39909:CDS | 30.0% | |
| TTCCATTCTATACTACTGTT+TGG | + | chr1.4:57899430-57899449 | MS.gene39909:intron | 30.0% | |
| !!! | TTATTTTAGCAACTCGATCA+CGG | + | chr1.4:57899453-57899472 | MS.gene39909:intron | 30.0% |
| AAGAACAACATACCAAGAAC+AGG | - | chr1.4:57899004-57899023 | None:intergenic | 35.0% | |
| AAGCAACTCAAACATTGGAA+TGG | + | chr1.4:57899732-57899751 | MS.gene39909:CDS | 35.0% | |
| ACATAGCAAAAACCACATGT+TGG | - | chr1.4:57899236-57899255 | None:intergenic | 35.0% | |
| AGCAACTCAAACATTGGAAT+GGG | + | chr1.4:57899733-57899752 | MS.gene39909:CDS | 35.0% | |
| CGTCAGTTTGATATTTGGTT+CGG | + | chr1.4:57899528-57899547 | MS.gene39909:intron | 35.0% | |
| GTAGTATAGAATGGAACGTT+CGG | - | chr1.4:57899426-57899445 | None:intergenic | 35.0% | |
| TGTCTCGTCAGTTTGATATT+TGG | + | chr1.4:57899523-57899542 | MS.gene39909:intron | 35.0% | |
| TTATTCAGGATTGCCAACAA+TGG | + | chr1.4:57899654-57899673 | MS.gene39909:intron | 35.0% | |
| !!! | CGGTTCAAGTTTTAAAACAC+TGG | - | chr1.4:57899406-57899425 | None:intergenic | 35.0% |
| AAATCAAGAGACGGTAGTCT+TGG | - | chr1.4:57899316-57899335 | None:intergenic | 40.0% | |
| ATTGTTGAAGCTCCAACATG+TGG | + | chr1.4:57899221-57899240 | MS.gene39909:intron | 40.0% | |
| CAAAAAGGATCCAACCAAGA+AGG | - | chr1.4:57899876-57899895 | None:intergenic | 40.0% | |
| CTTCCTTAAATCGCTCGAAT+CGG | - | chr1.4:57899486-57899505 | None:intergenic | 40.0% | |
| TAAGACCGAACACACCAAAA+AGG | - | chr1.4:57899891-57899910 | None:intergenic | 40.0% | |
| TTCCTTAAATCGCTCGAATC+GGG | - | chr1.4:57899485-57899504 | None:intergenic | 40.0% | |
| TTGCTTTCATTCACAGAAGG+TGG | - | chr1.4:57899718-57899737 | None:intergenic | 40.0% | |
| ! | ATGCCATCTAGTTTAGTACC+CGG | - | chr1.4:57899375-57899394 | None:intergenic | 40.0% |
| ! | CTTCTTGGTTGGATCCTTTT+TGG | + | chr1.4:57899874-57899893 | MS.gene39909:CDS | 40.0% |
| ! | GAGTTGCTTTCATTCACAGA+AGG | - | chr1.4:57899721-57899740 | None:intergenic | 40.0% |
| ! | TCTTTTGTGCAAGACATGCA+CGG | - | chr1.4:57898958-57898977 | None:intergenic | 40.0% |
| !! | GATGAAAGAATGAGCACTGA+AGG | + | chr1.4:57899820-57899839 | MS.gene39909:CDS | 40.0% |
| !! | TGGATCCTTTTTGGTGTGTT+CGG | + | chr1.4:57899883-57899902 | MS.gene39909:CDS | 40.0% |
| AAGGTTGTTGCTGAGTCCAA+AGG | - | chr1.4:57899857-57899876 | None:intergenic | 45.0% | |
| AGGATTGCCAACAATGGCTA+AGG | + | chr1.4:57899660-57899679 | MS.gene39909:intron | 45.0% | |
| AGGTTGTTGCTGAGTCCAAA+GGG | - | chr1.4:57899856-57899875 | None:intergenic | 45.0% | |
| AGTTGTAGTGGTAGCTGAGA+TGG | - | chr1.4:57898924-57898943 | None:intergenic | 45.0% | |
| ATTGCCAACAATGGCTAAGG+TGG | + | chr1.4:57899663-57899682 | MS.gene39909:CDS | 45.0% | |
| CAACATACCAAGAACAGGAG+AGG | - | chr1.4:57898999-57899018 | None:intergenic | 45.0% | |
| CTTGAAGAGGATGAAGAGTC+TGG | + | chr1.4:57899943-57899962 | MS.gene39909:CDS | 45.0% | |
| GTTTCATCCTCTCCTGTTCT+TGG | + | chr1.4:57898989-57899008 | MS.gene39909:CDS | 45.0% | |
| TCAGCAACAACCTTCTTGGT+TGG | + | chr1.4:57899863-57899882 | MS.gene39909:CDS | 45.0% | |
| TCTTTCATCCACCAATGCCA+TGG | - | chr1.4:57899809-57899828 | None:intergenic | 45.0% | |
| TTCATCCTCTTCAAGACTCG+AGG | - | chr1.4:57899938-57899957 | None:intergenic | 45.0% | |
| TTCCCGGGTACTAAACTAGA+TGG | + | chr1.4:57899369-57899388 | MS.gene39909:intron | 45.0% | |
| TTGCCAACAATGGCTAAGGT+GGG | + | chr1.4:57899664-57899683 | MS.gene39909:CDS | 45.0% | |
| ! | AAGAGTGAGGTGTTCAATGG+AGG | + | chr1.4:57899687-57899706 | MS.gene39909:CDS | 45.0% |
| ! | AGAGTGAGGTGTTCAATGGA+GGG | + | chr1.4:57899688-57899707 | MS.gene39909:CDS | 45.0% |
| ! | CTTTTGTGCAAGACATGCAC+GGG | - | chr1.4:57898957-57898976 | None:intergenic | 45.0% |
| ! | TGCCATCTAGTTTAGTACCC+GGG | - | chr1.4:57899374-57899393 | None:intergenic | 45.0% |
| ! | TGGTGTGTTCGGTCTTATCT+GGG | + | chr1.4:57899894-57899913 | MS.gene39909:CDS | 45.0% |
| ! | TTGGTGTGTTCGGTCTTATC+TGG | + | chr1.4:57899893-57899912 | MS.gene39909:CDS | 45.0% |
| !! | AGAATGAGCACTGAAGGAAC+CGG | + | chr1.4:57899826-57899845 | MS.gene39909:CDS | 45.0% |
| CTTCCCACCTTAGCCATTGT+TGG | - | chr1.4:57899670-57899689 | None:intergenic | 50.0% | |
| GGACTCAGCAACAACCTTCT+TGG | + | chr1.4:57899859-57899878 | MS.gene39909:CDS | 50.0% | |
| GGAGAGGATGAAACTGCAAG+AGG | - | chr1.4:57898983-57899002 | None:intergenic | 50.0% | |
| GGTGATCGATGCAGTTGTAG+TGG | - | chr1.4:57898936-57898955 | None:intergenic | 50.0% | |
| ! | GGGAAGAGTGAGGTGTTCAA+TGG | + | chr1.4:57899684-57899703 | MS.gene39909:CDS | 50.0% |
| ! | GTCCCGATTCGAGCGATTTA+AGG | + | chr1.4:57899480-57899499 | MS.gene39909:intron | 50.0% |
| !! | GAATGAGCACTGAAGGAACC+GGG | + | chr1.4:57899827-57899846 | MS.gene39909:CDS | 50.0% |
| CTACGCCTCGAGTCTTGAAG+AGG | + | chr1.4:57899930-57899949 | MS.gene39909:CDS | 55.0% | |
| TATGTCAAGCCCAGCAGCCA+TGG | + | chr1.4:57899789-57899808 | MS.gene39909:CDS | 55.0% | |
| TGGCTAAGGTGGGAAGAGTG+AGG | + | chr1.4:57899674-57899693 | MS.gene39909:CDS | 55.0% | |
| AAGCCCAGCAGCCATGGCAT+TGG | + | chr1.4:57899795-57899814 | MS.gene39909:CDS | 60.0% | |
| CCACCAATGCCATGGCTGCT+GGG | - | chr1.4:57899801-57899820 | None:intergenic | 60.0% | |
| GAAGGAACCGGGCTTCCCTT+TGG | + | chr1.4:57899838-57899857 | MS.gene39909:CDS | 60.0% | |
| GCTGAGTCCAAAGGGAAGCC+CGG | - | chr1.4:57899848-57899867 | None:intergenic | 60.0% | |
| TCCACCAATGCCATGGCTGC+TGG | - | chr1.4:57899802-57899821 | None:intergenic | 60.0% | |
| ! | CCCAGCAGCCATGGCATTGG+TGG | + | chr1.4:57899798-57899817 | MS.gene39909:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 57898914 | 57899978 | 57898914 | ID=MS.gene39909 |
| chr1.4 | mRNA | 57898914 | 57899978 | 57898914 | ID=MS.gene39909.t1;Parent=MS.gene39909 |
| chr1.4 | exon | 57898914 | 57899010 | 57898914 | ID=MS.gene39909.t1.exon1;Parent=MS.gene39909.t1 |
| chr1.4 | CDS | 57898914 | 57899010 | 57898914 | ID=cds.MS.gene39909.t1;Parent=MS.gene39909.t1 |
| chr1.4 | exon | 57899662 | 57899978 | 57899662 | ID=MS.gene39909.t1.exon2;Parent=MS.gene39909.t1 |
| chr1.4 | CDS | 57899662 | 57899978 | 57899662 | ID=cds.MS.gene39909.t1;Parent=MS.gene39909.t1 |
| Gene Sequence |
| Protein sequence |
