Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44398.t1 | RHN55120.1 | 89.7 | 253 | 26 | 0 | 1 | 253 | 172 | 424 | 4.20E-116 | 427.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44398.t1 | G7K2V9 | 89.7 | 253 | 26 | 0 | 1 | 253 | 171 | 423 | 3.0e-116 | 427.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene44398 | 0.807234 | 5.58E-50 | -1.69E-46 |
| MS.gene049150 | MS.gene44398 | 0.820694 | 5.99E-53 | -1.69E-46 |
| MS.gene049153 | MS.gene44398 | 0.824347 | 8.48E-54 | -1.69E-46 |
| MS.gene049260 | MS.gene44398 | 0.802511 | 5.42E-49 | -1.69E-46 |
| MS.gene049284 | MS.gene44398 | 0.817139 | 3.85E-52 | -1.69E-46 |
| MS.gene04933 | MS.gene44398 | 0.801276 | 9.72E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene44398 | 0.817167 | 3.79E-52 | -1.69E-46 |
| MS.gene049351 | MS.gene44398 | 0.879799 | 9.27E-70 | -1.69E-46 |
| MS.gene049356 | MS.gene44398 | 0.824591 | 7.43E-54 | -1.69E-46 |
| MS.gene049661 | MS.gene44398 | 0.831151 | 1.96E-55 | -1.69E-46 |
| MS.gene049662 | MS.gene44398 | 0.834468 | 2.95E-56 | -1.69E-46 |
| MS.gene049758 | MS.gene44398 | 0.862777 | 3.99E-64 | -1.69E-46 |
| MS.gene049910 | MS.gene44398 | 0.819695 | 1.01E-52 | -1.69E-46 |
| MS.gene050076 | MS.gene44398 | 0.813401 | 2.61E-51 | -1.69E-46 |
| MS.gene050099 | MS.gene44398 | 0.839329 | 1.69E-57 | -1.69E-46 |
| MS.gene050128 | MS.gene44398 | 0.820928 | 5.29E-53 | -1.69E-46 |
| MS.gene050231 | MS.gene44398 | 0.824365 | 8.40E-54 | -1.69E-46 |
| MS.gene050301 | MS.gene44398 | 0.892374 | 1.68E-74 | -1.69E-46 |
| MS.gene050397 | MS.gene44398 | 0.873981 | 9.65E-68 | -1.69E-46 |
| MS.gene050406 | MS.gene44398 | 0.824605 | 7.37E-54 | -1.69E-46 |
| MS.gene050426 | MS.gene44398 | -0.82973 | 4.37E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene44398 | 0.808226 | 3.44E-50 | -1.69E-46 |
| MS.gene050464 | MS.gene44398 | 0.875866 | 2.20E-68 | -1.69E-46 |
| MS.gene050529 | MS.gene44398 | 0.886266 | 3.97E-72 | -1.69E-46 |
| MS.gene050558 | MS.gene44398 | 0.823449 | 1.38E-53 | -1.69E-46 |
| MS.gene050642 | MS.gene44398 | 0.827491 | 1.52E-54 | -1.69E-46 |
| MS.gene050650 | MS.gene44398 | 0.819586 | 1.07E-52 | -1.69E-46 |
| MS.gene050670 | MS.gene44398 | 0.856858 | 2.42E-62 | -1.69E-46 |
| MS.gene050812 | MS.gene44398 | 0.836123 | 1.13E-56 | -1.69E-46 |
| MS.gene050971 | MS.gene44398 | 0.833304 | 5.76E-56 | -1.69E-46 |
| MS.gene05101 | MS.gene44398 | 0.853749 | 1.94E-61 | -1.69E-46 |
| MS.gene05105 | MS.gene44398 | 0.859622 | 3.64E-63 | -1.69E-46 |
| MS.gene051255 | MS.gene44398 | 0.809876 | 1.52E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene44398 | 0.803328 | 3.67E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene44398 | 0.84394 | 1.03E-58 | -1.69E-46 |
| MS.gene051525 | MS.gene44398 | 0.850007 | 2.24E-60 | -1.69E-46 |
| MS.gene051582 | MS.gene44398 | 0.81971 | 1.01E-52 | -1.69E-46 |
| MS.gene051663 | MS.gene44398 | 0.805436 | 1.34E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene44398 | 0.881346 | 2.59E-70 | -1.69E-46 |
| MS.gene051877 | MS.gene44398 | 0.823781 | 1.15E-53 | -1.69E-46 |
| MS.gene051983 | MS.gene44398 | 0.828353 | 9.43E-55 | -1.69E-46 |
| MS.gene052011 | MS.gene44398 | -0.857818 | 1.26E-62 | -1.69E-46 |
| MS.gene052077 | MS.gene44398 | 0.845256 | 4.55E-59 | -1.69E-46 |
| MS.gene052078 | MS.gene44398 | 0.880752 | 4.24E-70 | -1.69E-46 |
| MS.gene052178 | MS.gene44398 | 0.837233 | 5.86E-57 | -1.69E-46 |
| MS.gene052188 | MS.gene44398 | 0.891203 | 4.92E-74 | -1.69E-46 |
| MS.gene052207 | MS.gene44398 | 0.818087 | 2.35E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene44398 | 0.895158 | 1.25E-75 | -1.69E-46 |
| MS.gene052255 | MS.gene44398 | 0.81934 | 1.22E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene44398 | 0.871223 | 8.05E-67 | -1.69E-46 |
| MS.gene052322 | MS.gene44398 | 0.830862 | 2.31E-55 | -1.69E-46 |
| MS.gene052326 | MS.gene44398 | 0.818617 | 1.79E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene44398 | 0.87499 | 4.38E-68 | -1.69E-46 |
| MS.gene052414 | MS.gene44398 | 0.803664 | 3.13E-49 | -1.69E-46 |
| MS.gene052421 | MS.gene44398 | 0.806232 | 9.09E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene44398 | 0.843744 | 1.16E-58 | -1.69E-46 |
| MS.gene052484 | MS.gene44398 | 0.803662 | 3.13E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene44398 | 0.861015 | 1.38E-63 | -1.69E-46 |
| MS.gene052917 | MS.gene44398 | -0.803286 | 3.75E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene44398 | 0.82392 | 1.07E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene44398 | 0.832172 | 1.10E-55 | -1.69E-46 |
| MS.gene053209 | MS.gene44398 | 0.862144 | 6.24E-64 | -1.69E-46 |
| MS.gene053557 | MS.gene44398 | 0.849 | 4.28E-60 | -1.69E-46 |
| MS.gene053758 | MS.gene44398 | 0.815628 | 8.39E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene44398 | 0.882733 | 8.13E-71 | -1.69E-46 |
| MS.gene053928 | MS.gene44398 | 0.844 | 9.91E-59 | -1.69E-46 |
| MS.gene053971 | MS.gene44398 | 0.813024 | 3.16E-51 | -1.69E-46 |
| MS.gene054010 | MS.gene44398 | 0.836516 | 8.94E-57 | -1.69E-46 |
| MS.gene054011 | MS.gene44398 | 0.849547 | 3.01E-60 | -1.69E-46 |
| MS.gene054012 | MS.gene44398 | 0.851484 | 8.61E-61 | -1.69E-46 |
| MS.gene054013 | MS.gene44398 | 0.847725 | 9.64E-60 | -1.69E-46 |
| MS.gene054123 | MS.gene44398 | 0.8394 | 1.62E-57 | -1.69E-46 |
| MS.gene054183 | MS.gene44398 | 0.842639 | 2.29E-58 | -1.69E-46 |
| MS.gene054349 | MS.gene44398 | 0.839211 | 1.81E-57 | -1.69E-46 |
| MS.gene054473 | MS.gene44398 | 0.836955 | 6.91E-57 | -1.69E-46 |
| MS.gene054556 | MS.gene44398 | 0.82352 | 1.33E-53 | -1.69E-46 |
| MS.gene05455 | MS.gene44398 | 0.825309 | 5.03E-54 | -1.69E-46 |
| MS.gene054719 | MS.gene44398 | 0.841866 | 3.66E-58 | -1.69E-46 |
| MS.gene054955 | MS.gene44398 | 0.808979 | 2.37E-50 | -1.69E-46 |
| MS.gene054983 | MS.gene44398 | 0.832078 | 1.16E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene44398 | 0.868474 | 6.36E-66 | -1.69E-46 |
| MS.gene055277 | MS.gene44398 | 0.875422 | 3.12E-68 | -1.69E-46 |
| MS.gene055390 | MS.gene44398 | 0.841807 | 3.80E-58 | -1.69E-46 |
| MS.gene055412 | MS.gene44398 | 0.818888 | 1.55E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene44398 | 0.887056 | 1.99E-72 | -1.69E-46 |
| MS.gene055428 | MS.gene44398 | 0.874687 | 5.56E-68 | -1.69E-46 |
| MS.gene05553 | MS.gene44398 | 0.83124 | 1.87E-55 | -1.69E-46 |
| MS.gene055604 | MS.gene44398 | 0.862721 | 4.15E-64 | -1.69E-46 |
| MS.gene05602 | MS.gene44398 | 0.800959 | 1.13E-48 | -1.69E-46 |
| MS.gene056191 | MS.gene44398 | 0.857843 | 1.24E-62 | -1.69E-46 |
| MS.gene056314 | MS.gene44398 | 0.807549 | 4.79E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene44398 | 0.85792 | 1.17E-62 | -1.69E-46 |
| MS.gene056410 | MS.gene44398 | 0.831109 | 2.01E-55 | -1.69E-46 |
| MS.gene056511 | MS.gene44398 | 0.801202 | 1.01E-48 | -1.69E-46 |
| MS.gene056609 | MS.gene44398 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene44398 | 0.864752 | 9.70E-65 | -1.69E-46 |
| MS.gene056659 | MS.gene44398 | 0.850807 | 1.34E-60 | -1.69E-46 |
| MS.gene056723 | MS.gene44398 | 0.812693 | 3.73E-51 | -1.69E-46 |
| MS.gene056796 | MS.gene44398 | 0.855626 | 5.56E-62 | -1.69E-46 |
| MS.gene056899 | MS.gene44398 | -0.838807 | 2.31E-57 | -1.69E-46 |
| MS.gene056902 | MS.gene44398 | 0.839877 | 1.22E-57 | -1.69E-46 |
| MS.gene056979 | MS.gene44398 | 0.826133 | 3.21E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene44398 | 0.850058 | 2.17E-60 | -1.69E-46 |
| MS.gene057121 | MS.gene44398 | 0.864541 | 1.13E-64 | -1.69E-46 |
| MS.gene057124 | MS.gene44398 | 0.825429 | 4.71E-54 | -1.69E-46 |
| MS.gene057146 | MS.gene44398 | 0.811136 | 8.14E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene44398 | 0.825966 | 3.51E-54 | -1.69E-46 |
| MS.gene05733 | MS.gene44398 | 0.842852 | 2.01E-58 | -1.69E-46 |
| MS.gene057396 | MS.gene44398 | 0.835357 | 1.76E-56 | -1.69E-46 |
| MS.gene057439 | MS.gene44398 | 0.824975 | 6.03E-54 | -1.69E-46 |
| MS.gene057448 | MS.gene44398 | 0.826552 | 2.55E-54 | -1.69E-46 |
| MS.gene057449 | MS.gene44398 | 0.805708 | 1.17E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene44398 | 0.825549 | 4.41E-54 | -1.69E-46 |
| MS.gene057469 | MS.gene44398 | 0.823339 | 1.46E-53 | -1.69E-46 |
| MS.gene057476 | MS.gene44398 | 0.801658 | 8.12E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene44398 | 0.869356 | 3.29E-66 | -1.69E-46 |
| MS.gene057796 | MS.gene44398 | 0.821897 | 3.16E-53 | -1.69E-46 |
| MS.gene057823 | MS.gene44398 | 0.873914 | 1.02E-67 | -1.69E-46 |
| MS.gene058002 | MS.gene44398 | 0.88839 | 6.16E-73 | -1.69E-46 |
| MS.gene058007 | MS.gene44398 | 0.805759 | 1.14E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene44398 | 0.826483 | 2.65E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene44398 | 0.911804 | 4.01E-83 | -1.69E-46 |
| MS.gene058142 | MS.gene44398 | 0.862532 | 4.74E-64 | -1.69E-46 |
| MS.gene058233 | MS.gene44398 | 0.852773 | 3.71E-61 | -1.69E-46 |
| MS.gene058276 | MS.gene44398 | 0.85435 | 1.30E-61 | -1.69E-46 |
| MS.gene058293 | MS.gene44398 | 0.851171 | 1.06E-60 | -1.69E-46 |
| MS.gene058365 | MS.gene44398 | 0.819253 | 1.28E-52 | -1.69E-46 |
| MS.gene058376 | MS.gene44398 | 0.840908 | 6.55E-58 | -1.69E-46 |
| MS.gene05842 | MS.gene44398 | 0.809146 | 2.19E-50 | -1.69E-46 |
| MS.gene058444 | MS.gene44398 | 0.823236 | 1.54E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene44398 | 0.880703 | 4.41E-70 | -1.69E-46 |
| MS.gene058700 | MS.gene44398 | 0.824259 | 8.89E-54 | -1.69E-46 |
| MS.gene058710 | MS.gene44398 | 0.845798 | 3.24E-59 | -1.69E-46 |
| MS.gene058749 | MS.gene44398 | 0.817828 | 2.69E-52 | -1.69E-46 |
| MS.gene058787 | MS.gene44398 | 0.828721 | 7.68E-55 | -1.69E-46 |
| MS.gene058845 | MS.gene44398 | 0.829466 | 5.07E-55 | -1.69E-46 |
| MS.gene05886 | MS.gene44398 | 0.833492 | 5.17E-56 | -1.69E-46 |
| MS.gene058928 | MS.gene44398 | 0.865336 | 6.36E-65 | -1.69E-46 |
| MS.gene058993 | MS.gene44398 | 0.805025 | 1.63E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene44398 | 0.892512 | 1.48E-74 | -1.69E-46 |
| MS.gene059141 | MS.gene44398 | 0.833855 | 4.20E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene44398 | 0.848577 | 5.61E-60 | -1.69E-46 |
| MS.gene059294 | MS.gene44398 | 0.862955 | 3.51E-64 | -1.69E-46 |
| MS.gene059342 | MS.gene44398 | 0.820744 | 5.83E-53 | -1.69E-46 |
| MS.gene059366 | MS.gene44398 | 0.846032 | 2.80E-59 | -1.69E-46 |
| MS.gene059420 | MS.gene44398 | 0.879844 | 8.94E-70 | -1.69E-46 |
| MS.gene059518 | MS.gene44398 | 0.867262 | 1.56E-65 | -1.69E-46 |
| MS.gene059536 | MS.gene44398 | 0.821887 | 3.18E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene44398 | 0.873064 | 1.96E-67 | -1.69E-46 |
| MS.gene05959 | MS.gene44398 | 0.81277 | 3.59E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene44398 | 0.859791 | 3.24E-63 | -1.69E-46 |
| MS.gene059688 | MS.gene44398 | 0.876844 | 1.01E-68 | -1.69E-46 |
| MS.gene059692 | MS.gene44398 | 0.880092 | 7.29E-70 | -1.69E-46 |
| MS.gene059695 | MS.gene44398 | 0.883037 | 6.30E-71 | -1.69E-46 |
| MS.gene059807 | MS.gene44398 | 0.804192 | 2.43E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene44398 | 0.915539 | 5.22E-85 | -1.69E-46 |
| MS.gene059894 | MS.gene44398 | 0.866886 | 2.05E-65 | -1.69E-46 |
| MS.gene059974 | MS.gene44398 | 0.815278 | 1.00E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene44398 | 0.839995 | 1.13E-57 | -1.69E-46 |
| MS.gene060403 | MS.gene44398 | 0.822422 | 2.39E-53 | -1.69E-46 |
| MS.gene060407 | MS.gene44398 | 0.83309 | 6.51E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene44398 | 0.824387 | 8.30E-54 | -1.69E-46 |
| MS.gene06090 | MS.gene44398 | 0.828745 | 7.58E-55 | -1.69E-46 |
| MS.gene060986 | MS.gene44398 | 0.843089 | 1.74E-58 | -1.69E-46 |
| MS.gene060994 | MS.gene44398 | 0.830953 | 2.20E-55 | -1.69E-46 |
| MS.gene061000 | MS.gene44398 | 0.82577 | 3.91E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene44398 | 0.889079 | 3.34E-73 | -1.69E-46 |
| MS.gene061051 | MS.gene44398 | -0.822864 | 1.89E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene44398 | 0.861884 | 7.50E-64 | -1.69E-46 |
| MS.gene061159 | MS.gene44398 | 0.809636 | 1.72E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGAAAGAGAACGCGAAAA+AGG | 0.306013 | 5.4:-25071717 | MS.gene44398:CDS |
| AATATAGTCCCTGCTGTTAT+CGG | 0.320672 | 5.4:-25072661 | MS.gene44398:CDS |
| TTCAAACGAGGATTCCAATA+TGG | 0.378780 | 5.4:-25071984 | MS.gene44398:CDS |
| CTGGTGCAGGTTAAAGATTA+AGG | 0.388699 | 5.4:-25071576 | MS.gene44398:intron |
| ACAGGACAAACTATTACATT+TGG | 0.404371 | 5.4:-25072121 | MS.gene44398:CDS |
| TGAGACTGAAGTAGATGATT+CGG | 0.416272 | 5.4:-25071936 | MS.gene44398:CDS |
| GAAGGCAGAATCATCCATAT+TGG | 0.419468 | 5.4:+25071970 | None:intergenic |
| GCACAGTCTTCCTCTTCTCC+TGG | 0.426196 | 5.4:-25072061 | MS.gene44398:CDS |
| TCACCAAGCGCACTCGGCTT+CGG | 0.431225 | 5.4:+25072752 | None:intergenic |
| ATGTTCCTGGATCTGCAAAA+AGG | 0.434218 | 5.4:-25071688 | MS.gene44398:intron |
| CTCGTTTGAATCTTCTTCAA+AGG | 0.444522 | 5.4:+25071997 | None:intergenic |
| AAAAGCTGCCCTTGCTTCAA+AGG | 0.445602 | 5.4:-25071513 | MS.gene44398:CDS |
| TTCTTGTTTCCGATAACAGC+AGG | 0.456122 | 5.4:+25072652 | None:intergenic |
| AAGCGCACTCGGCTTCGGAC+TGG | 0.457622 | 5.4:+25072757 | None:intergenic |
| ATGATTCTGCCTTCGATACA+TGG | 0.458850 | 5.4:-25071961 | MS.gene44398:CDS |
| GGGACTATATTTGTTATGCC+TGG | 0.464583 | 5.4:+25072673 | None:intergenic |
| GATGACTTCTTCTCAGCTGT+GGG | 0.467868 | 5.4:+25071782 | None:intergenic |
| CAGGTTAAAGATTAAGGAAA+AGG | 0.472906 | 5.4:-25071570 | MS.gene44398:CDS |
| AGATGATTCGGATGAGCTAC+AGG | 0.478552 | 5.4:-25071924 | MS.gene44398:CDS |
| AGATGACTTCTTCTCAGCTG+TGG | 0.479354 | 5.4:+25071781 | None:intergenic |
| AGTCCGAAGCCGAGTGCGCT+TGG | 0.479878 | 5.4:-25072755 | MS.gene44398:CDS |
| CAAATCACCTCTCTCTAAGC+AGG | 0.501806 | 5.4:-25071816 | MS.gene44398:CDS |
| GTAAATTCACCAAGCGCACT+CGG | 0.528586 | 5.4:+25072746 | None:intergenic |
| AGCATGAAGACGATGCAGTT+AGG | 0.534347 | 5.4:-25072585 | MS.gene44398:intron |
| GTTTGAATCTTCTTCAAAGG+AGG | 0.538215 | 5.4:+25072000 | None:intergenic |
| TTTGAATCTTCTTCAAAGGA+GGG | 0.542082 | 5.4:+25072001 | None:intergenic |
| ATGAGCTACAGGAAAGTGAC+GGG | 0.544165 | 5.4:-25071913 | MS.gene44398:CDS |
| GACAAGGGATCCTGTCTGCA+AGG | 0.554772 | 5.4:-25072629 | MS.gene44398:CDS |
| AAAGCAAAACCCTTGCAGAC+AGG | 0.557225 | 5.4:+25072619 | None:intergenic |
| TCTTCAAAGGAGGGCAAGAG+AGG | 0.560364 | 5.4:+25072010 | None:intergenic |
| CTGTCAACCTGCTTAGAGAG+AGG | 0.566700 | 5.4:+25071809 | None:intergenic |
| CTAACTCTCCTTTGAAGCAA+GGG | 0.576199 | 5.4:+25071505 | None:intergenic |
| TTTGTTTAAACCAGGAGAAG+AGG | 0.577338 | 5.4:+25072051 | None:intergenic |
| ACTAACTCTCCTTTGAAGCA+AGG | 0.584876 | 5.4:+25071504 | None:intergenic |
| CTTTGAAGAAGATTCAAACG+AGG | 0.612905 | 5.4:-25071996 | MS.gene44398:CDS |
| GATGAGCTACAGGAAAGTGA+CGG | 0.627818 | 5.4:-25071914 | MS.gene44398:CDS |
| ACAAGGGATCCTGTCTGCAA+GGG | 0.629959 | 5.4:-25072628 | MS.gene44398:CDS |
| AGTCTCATCCCATGTATCGA+AGG | 0.630276 | 5.4:+25071952 | None:intergenic |
| TCTTGTTTCCGATAACAGCA+GGG | 0.632817 | 5.4:+25072653 | None:intergenic |
| TGATTCTGCCTTCGATACAT+GGG | 0.636318 | 5.4:-25071960 | MS.gene44398:CDS |
| TTATCGGAAACAAGAAGACA+AGG | 0.645166 | 5.4:-25072645 | MS.gene44398:CDS |
| GTTCGCCACTGCACTGACTG+TGG | 0.667428 | 5.4:-25071879 | MS.gene44398:CDS |
| ACTATCCACAGTCAGTGCAG+TGG | 0.670660 | 5.4:+25071874 | None:intergenic |
| AGGTTTGTAGAGATATACAC+AGG | 0.675722 | 5.4:-25072139 | MS.gene44398:CDS |
| TATCGGAAACAAGAAGACAA+GGG | 0.712924 | 5.4:-25072644 | MS.gene44398:CDS |
| TGAGCTACAGGAAAGTGACG+GGG | 0.745988 | 5.4:-25071912 | MS.gene44398:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTATAATAATAGAAAATAA+GGG | - | chr5.4:25071731-25071750 | MS.gene44398:CDS | 10.0% |
| !! | TCTTATAATAATAGAAAATA+AGG | - | chr5.4:25071730-25071749 | MS.gene44398:CDS | 10.0% |
| !!! | AGTAAAATGTTTTAATAACA+TGG | + | chr5.4:25072037-25072056 | None:intergenic | 15.0% |
| !!! | GTAAAATGTTTTAATAACAT+GGG | + | chr5.4:25072036-25072055 | None:intergenic | 15.0% |
| !!! | TATTAAAACATTTTACTTCT+TGG | - | chr5.4:25072040-25072059 | MS.gene44398:CDS | 15.0% |
| !! | TGCATGACATTATTATAATA+TGG | - | chr5.4:25071782-25071801 | MS.gene44398:CDS | 20.0% |
| !!! | TTTACAAGTTCACATTTTAT+CGG | + | chr5.4:25072194-25072213 | None:intergenic | 20.0% |
| ! | ATATGGAAAAAGTTTCACAT+TGG | - | chr5.4:25071799-25071818 | MS.gene44398:CDS | 25.0% |
| ! | GAAAAATCTGTGTAATTTAG+CGG | - | chr5.4:25071893-25071912 | MS.gene44398:CDS | 25.0% |
| !! | CTGTCAATTTTTCTTTTCTT+TGG | + | chr5.4:25071555-25071574 | None:intergenic | 25.0% |
| !!! | ATTTGAAATCAGCTTTTCAA+GGG | - | chr5.4:25071577-25071596 | MS.gene44398:intron | 25.0% |
| !!! | TCGTACTAGATTTTTTCTTA+GGG | + | chr5.4:25072544-25072563 | None:intergenic | 25.0% |
| !!! | TTCGTACTAGATTTTTTCTT+AGG | + | chr5.4:25072545-25072564 | None:intergenic | 25.0% |
| !!! | TTGAGATTTTTGTTTAAACC+AGG | + | chr5.4:25072236-25072255 | None:intergenic | 25.0% |
| ACAGGACAAACTATTACATT+TGG | - | chr5.4:25072155-25072174 | MS.gene44398:intron | 30.0% | |
| CAGGTTAAAGATTAAGGAAA+AGG | - | chr5.4:25072706-25072725 | MS.gene44398:CDS | 30.0% | |
| TTTGAATCTTCTTCAAAGGA+GGG | + | chr5.4:25072278-25072297 | None:intergenic | 30.0% | |
| ! | AAATAAGGGAAGAGTATGTT+TGG | - | chr5.4:25071745-25071764 | MS.gene44398:CDS | 30.0% |
| ! | TTCTCATGTATGATTGATTC+TGG | - | chr5.4:25072681-25072700 | MS.gene44398:CDS | 30.0% |
| !! | GATTTGAAATCAGCTTTTCA+AGG | - | chr5.4:25071576-25071595 | MS.gene44398:intron | 30.0% |
| !!! | AAAAAGGTTTTAGATGTTCC+TGG | - | chr5.4:25072575-25072594 | MS.gene44398:intron | 30.0% |
| !!! | CGTACTAGATTTTTTCTTAG+GGG | + | chr5.4:25072543-25072562 | None:intergenic | 30.0% |
| AATATAGTCCCTGCTGTTAT+CGG | - | chr5.4:25071615-25071634 | MS.gene44398:intron | 35.0% | |
| AGGTTTGTAGAGATATACAC+AGG | - | chr5.4:25072137-25072156 | MS.gene44398:CDS | 35.0% | |
| CTCGTTTGAATCTTCTTCAA+AGG | + | chr5.4:25072282-25072301 | None:intergenic | 35.0% | |
| GTTTGAATCTTCTTCAAAGG+AGG | + | chr5.4:25072279-25072298 | None:intergenic | 35.0% | |
| TATCGGAAACAAGAAGACAA+GGG | - | chr5.4:25071632-25071651 | MS.gene44398:intron | 35.0% | |
| TGAGACTGAAGTAGATGATT+CGG | - | chr5.4:25072340-25072359 | MS.gene44398:intron | 35.0% | |
| TTATCGGAAACAAGAAGACA+AGG | - | chr5.4:25071631-25071650 | MS.gene44398:intron | 35.0% | |
| TTCAAACGAGGATTCCAATA+TGG | - | chr5.4:25072292-25072311 | MS.gene44398:intron | 35.0% | |
| TTTGTTTAAACCAGGAGAAG+AGG | + | chr5.4:25072228-25072247 | None:intergenic | 35.0% | |
| ! | AGTGATGTTTTCTCCAAGTA+CGG | - | chr5.4:25072737-25072756 | MS.gene44398:CDS | 35.0% |
| ! | CTTTGAAGAAGATTCAAACG+AGG | - | chr5.4:25072280-25072299 | MS.gene44398:intron | 35.0% |
| ! | GGAAGATGGTTTTCTATTCT+TGG | - | chr5.4:25071914-25071933 | MS.gene44398:CDS | 35.0% |
| ATGATTCTGCCTTCGATACA+TGG | - | chr5.4:25072315-25072334 | MS.gene44398:intron | 40.0% | |
| ATGTTCCTGGATCTGCAAAA+AGG | - | chr5.4:25072588-25072607 | MS.gene44398:CDS | 40.0% | |
| CTGGTGCAGGTTAAAGATTA+AGG | - | chr5.4:25072700-25072719 | MS.gene44398:CDS | 40.0% | |
| CTGTGTAATTTAGCGGAAGA+TGG | - | chr5.4:25071900-25071919 | MS.gene44398:CDS | 40.0% | |
| GAAGGCAGAATCATCCATAT+TGG | + | chr5.4:25072309-25072328 | None:intergenic | 40.0% | |
| GATTCACTGTGCATCATAGT+TGG | - | chr5.4:25072612-25072631 | MS.gene44398:CDS | 40.0% | |
| TACGAAAGAGAACGCGAAAA+AGG | - | chr5.4:25072559-25072578 | MS.gene44398:intron | 40.0% | |
| TGATTCTGCCTTCGATACAT+GGG | - | chr5.4:25072316-25072335 | MS.gene44398:intron | 40.0% | |
| ! | AATCACCTTTTTGCAGATCC+AGG | + | chr5.4:25072596-25072615 | None:intergenic | 40.0% |
| ! | TCTTGTTTCCGATAACAGCA+GGG | + | chr5.4:25071626-25071645 | None:intergenic | 40.0% |
| ! | TGTATGATTGATTCTGGTGC+AGG | - | chr5.4:25072687-25072706 | MS.gene44398:CDS | 40.0% |
| ! | TTCTTGTTTCCGATAACAGC+AGG | + | chr5.4:25071627-25071646 | None:intergenic | 40.0% |
| !! | CTAACTCTCCTTTGAAGCAA+GGG | + | chr5.4:25072774-25072793 | None:intergenic | 40.0% |
| !! | GGGACTATATTTGTTATGCC+TGG | + | chr5.4:25071606-25071625 | None:intergenic | 40.0% |
| AAAAGCTGCCCTTGCTTCAA+AGG | - | chr5.4:25072763-25072782 | MS.gene44398:CDS | 45.0% | |
| AAAGCAAAACCCTTGCAGAC+AGG | + | chr5.4:25071660-25071679 | None:intergenic | 45.0% | |
| AGATGACTTCTTCTCAGCTG+TGG | + | chr5.4:25072498-25072517 | None:intergenic | 45.0% | |
| AGCATGAAGACGATGCAGTT+AGG | - | chr5.4:25071691-25071710 | MS.gene44398:CDS | 45.0% | |
| AGTCTCATCCCATGTATCGA+AGG | + | chr5.4:25072327-25072346 | None:intergenic | 45.0% | |
| ATGAGCTACAGGAAAGTGAC+GGG | - | chr5.4:25072363-25072382 | MS.gene44398:intron | 45.0% | |
| CAAATCACCTCTCTCTAAGC+AGG | - | chr5.4:25072460-25072479 | MS.gene44398:intron | 45.0% | |
| GAGTGTATGTGTGTAGCATC+TGG | - | chr5.4:25071986-25072005 | MS.gene44398:CDS | 45.0% | |
| GATGACTTCTTCTCAGCTGT+GGG | + | chr5.4:25072497-25072516 | None:intergenic | 45.0% | |
| GATGAGCTACAGGAAAGTGA+CGG | - | chr5.4:25072362-25072381 | MS.gene44398:intron | 45.0% | |
| GTAAATTCACCAAGCGCACT+CGG | + | chr5.4:25071533-25071552 | None:intergenic | 45.0% | |
| TCACTTGCTCTCTCTTCTTC+AGG | - | chr5.4:25072117-25072136 | MS.gene44398:CDS | 45.0% | |
| ! | AGATGATTCGGATGAGCTAC+AGG | - | chr5.4:25072352-25072371 | MS.gene44398:intron | 45.0% |
| !! | CTGCAAGGGTTTTGCTTTTG+TGG | - | chr5.4:25071662-25071681 | MS.gene44398:intron | 45.0% |
| !! | TCAGCTTTTCAAGGGATTCC+AGG | - | chr5.4:25071585-25071604 | MS.gene44398:intron | 45.0% |
| ACAAGGGATCCTGTCTGCAA+GGG | - | chr5.4:25071648-25071667 | MS.gene44398:intron | 50.0% | |
| ACTATCCACAGTCAGTGCAG+TGG | + | chr5.4:25072405-25072424 | None:intergenic | 50.0% | |
| CTGTCAACCTGCTTAGAGAG+AGG | + | chr5.4:25072470-25072489 | None:intergenic | 50.0% | |
| TCTTCAAAGGAGGGCAAGAG+AGG | + | chr5.4:25072269-25072288 | None:intergenic | 50.0% | |
| TGAGCTACAGGAAAGTGACG+GGG | - | chr5.4:25072364-25072383 | MS.gene44398:intron | 50.0% | |
| ! | GGCAGCTTTTGATCCGTACT+TGG | + | chr5.4:25072753-25072772 | None:intergenic | 50.0% |
| GACAAGGGATCCTGTCTGCA+AGG | - | chr5.4:25071647-25071666 | MS.gene44398:intron | 55.0% | |
| GCACAGTCTTCCTCTTCTCC+TGG | - | chr5.4:25072215-25072234 | MS.gene44398:intron | 55.0% | |
| TCACCAAGCGCACTCGGCTT+CGG | + | chr5.4:25071527-25071546 | None:intergenic | 60.0% | |
| !! | GTTCGCCACTGCACTGACTG+TGG | - | chr5.4:25072397-25072416 | MS.gene44398:intron | 60.0% |
| AAGCGCACTCGGCTTCGGAC+TGG | + | chr5.4:25071522-25071541 | None:intergenic | 65.0% | |
| AGTCCGAAGCCGAGTGCGCT+TGG | - | chr5.4:25071521-25071540 | MS.gene44398:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 25071505 | 25072793 | 25071505 | ID=MS.gene44398 |
| chr5.4 | mRNA | 25071505 | 25072793 | 25071505 | ID=MS.gene44398.t1;Parent=MS.gene44398 |
| chr5.4 | exon | 25072586 | 25072793 | 25072586 | ID=MS.gene44398.t1.exon1;Parent=MS.gene44398.t1 |
| chr5.4 | CDS | 25072586 | 25072793 | 25072586 | ID=cds.MS.gene44398.t1;Parent=MS.gene44398.t1 |
| chr5.4 | exon | 25071689 | 25072159 | 25071689 | ID=MS.gene44398.t1.exon2;Parent=MS.gene44398.t1 |
| chr5.4 | CDS | 25071689 | 25072159 | 25071689 | ID=cds.MS.gene44398.t1;Parent=MS.gene44398.t1 |
| chr5.4 | exon | 25071505 | 25071589 | 25071505 | ID=MS.gene44398.t1.exon3;Parent=MS.gene44398.t1 |
| chr5.4 | CDS | 25071505 | 25071589 | 25071505 | ID=cds.MS.gene44398.t1;Parent=MS.gene44398.t1 |
| Gene Sequence |
| Protein sequence |
