Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48509.t1 | XP_003603045.2 | 93.2 | 162 | 8 | 1 | 1 | 159 | 57 | 218 | 5.90E-76 | 293.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48509.t1 | Q9C5C5 | 77.4 | 124 | 28 | 0 | 31 | 154 | 81 | 204 | 1.3e-52 | 207.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48509.t1 | G7J6D9 | 93.2 | 162 | 8 | 1 | 1 | 159 | 57 | 218 | 4.2e-76 | 293.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene48509 | 0.801997 | 6.91E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene48509 | 0.833339 | 5.65E-56 | -1.69E-46 |
| MS.gene049356 | MS.gene48509 | 0.825082 | 5.69E-54 | -1.69E-46 |
| MS.gene049389 | MS.gene48509 | 0.806271 | 8.92E-50 | -1.69E-46 |
| MS.gene049491 | MS.gene48509 | 0.836532 | 8.85E-57 | -1.69E-46 |
| MS.gene049661 | MS.gene48509 | 0.850955 | 1.22E-60 | -1.69E-46 |
| MS.gene049662 | MS.gene48509 | 0.845649 | 3.56E-59 | -1.69E-46 |
| MS.gene049758 | MS.gene48509 | 0.839504 | 1.52E-57 | -1.69E-46 |
| MS.gene049805 | MS.gene48509 | 0.806304 | 8.78E-50 | -1.69E-46 |
| MS.gene049910 | MS.gene48509 | 0.806963 | 6.37E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene48509 | 0.840218 | 9.93E-58 | -1.69E-46 |
| MS.gene050099 | MS.gene48509 | 0.80468 | 1.92E-49 | -1.69E-46 |
| MS.gene050128 | MS.gene48509 | 0.812829 | 3.48E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene48509 | 0.877617 | 5.44E-69 | -1.69E-46 |
| MS.gene050397 | MS.gene48509 | 0.873446 | 1.46E-67 | -1.69E-46 |
| MS.gene050400 | MS.gene48509 | 0.814026 | 1.90E-51 | -1.69E-46 |
| MS.gene050404 | MS.gene48509 | 0.817445 | 3.29E-52 | -1.69E-46 |
| MS.gene050406 | MS.gene48509 | 0.838602 | 2.61E-57 | -1.69E-46 |
| MS.gene050432 | MS.gene48509 | 0.822869 | 1.88E-53 | -1.69E-46 |
| MS.gene050464 | MS.gene48509 | 0.872139 | 4.00E-67 | -1.69E-46 |
| MS.gene050529 | MS.gene48509 | 0.867519 | 1.29E-65 | -1.69E-46 |
| MS.gene05053 | MS.gene48509 | 0.82535 | 4.92E-54 | -1.69E-46 |
| MS.gene050593 | MS.gene48509 | 0.817427 | 3.32E-52 | -1.69E-46 |
| MS.gene050670 | MS.gene48509 | 0.881091 | 3.20E-70 | -1.69E-46 |
| MS.gene050767 | MS.gene48509 | 0.806354 | 8.57E-50 | -1.69E-46 |
| MS.gene050770 | MS.gene48509 | 0.809147 | 2.18E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene48509 | 0.803123 | 4.05E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene48509 | 0.812731 | 3.66E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene48509 | 0.852701 | 3.88E-61 | -1.69E-46 |
| MS.gene05105 | MS.gene48509 | 0.878398 | 2.90E-69 | -1.69E-46 |
| MS.gene051360 | MS.gene48509 | 0.806719 | 7.18E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene48509 | 0.83203 | 1.19E-55 | -1.69E-46 |
| MS.gene051471 | MS.gene48509 | 0.832581 | 8.71E-56 | -1.69E-46 |
| MS.gene051525 | MS.gene48509 | 0.838509 | 2.76E-57 | -1.69E-46 |
| MS.gene051582 | MS.gene48509 | 0.823238 | 1.54E-53 | -1.69E-46 |
| MS.gene051605 | MS.gene48509 | 0.813335 | 2.70E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene48509 | 0.820327 | 7.27E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene48509 | 0.820634 | 6.18E-53 | -1.69E-46 |
| MS.gene051858 | MS.gene48509 | 0.803023 | 4.25E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene48509 | 0.833404 | 5.44E-56 | -1.69E-46 |
| MS.gene051877 | MS.gene48509 | 0.822501 | 2.29E-53 | -1.69E-46 |
| MS.gene052011 | MS.gene48509 | -0.821691 | 3.53E-53 | -1.69E-46 |
| MS.gene052077 | MS.gene48509 | 0.836261 | 1.04E-56 | -1.69E-46 |
| MS.gene052078 | MS.gene48509 | 0.861295 | 1.13E-63 | -1.69E-46 |
| MS.gene052178 | MS.gene48509 | 0.81663 | 5.01E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene48509 | 0.884499 | 1.82E-71 | -1.69E-46 |
| MS.gene052253 | MS.gene48509 | 0.867255 | 1.57E-65 | -1.69E-46 |
| MS.gene052290 | MS.gene48509 | 0.819374 | 1.20E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene48509 | 0.901068 | 3.89E-78 | -1.69E-46 |
| MS.gene052322 | MS.gene48509 | 0.831516 | 1.60E-55 | -1.69E-46 |
| MS.gene052326 | MS.gene48509 | 0.814982 | 1.17E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene48509 | 0.873872 | 1.05E-67 | -1.69E-46 |
| MS.gene052421 | MS.gene48509 | 0.835989 | 1.22E-56 | -1.69E-46 |
| MS.gene052481 | MS.gene48509 | 0.829324 | 5.49E-55 | -1.69E-46 |
| MS.gene052484 | MS.gene48509 | 0.82471 | 6.97E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene48509 | 0.84053 | 8.23E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene48509 | 0.883223 | 5.38E-71 | -1.69E-46 |
| MS.gene053104 | MS.gene48509 | 0.8371 | 6.34E-57 | -1.69E-46 |
| MS.gene05316 | MS.gene48509 | 0.804812 | 1.80E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene48509 | 0.866905 | 2.02E-65 | -1.69E-46 |
| MS.gene053322 | MS.gene48509 | 0.823612 | 1.26E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene48509 | 0.825965 | 3.52E-54 | -1.69E-46 |
| MS.gene053557 | MS.gene48509 | 0.859558 | 3.81E-63 | -1.69E-46 |
| MS.gene053836 | MS.gene48509 | 0.857525 | 1.54E-62 | -1.69E-46 |
| MS.gene053912 | MS.gene48509 | 0.80183 | 7.48E-49 | -1.69E-46 |
| MS.gene054010 | MS.gene48509 | 0.805757 | 1.14E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene48509 | 0.812935 | 3.30E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene48509 | 0.803406 | 3.54E-49 | -1.69E-46 |
| MS.gene054013 | MS.gene48509 | 0.801038 | 1.09E-48 | -1.69E-46 |
| MS.gene054123 | MS.gene48509 | 0.846527 | 2.05E-59 | -1.69E-46 |
| MS.gene054349 | MS.gene48509 | 0.831715 | 1.43E-55 | -1.69E-46 |
| MS.gene054376 | MS.gene48509 | 0.82488 | 6.35E-54 | -1.69E-46 |
| MS.gene054473 | MS.gene48509 | 0.800426 | 1.45E-48 | -1.69E-46 |
| MS.gene05455 | MS.gene48509 | 0.815024 | 1.14E-51 | -1.69E-46 |
| MS.gene054802 | MS.gene48509 | 0.810044 | 1.40E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene48509 | 0.808617 | 2.84E-50 | -1.69E-46 |
| MS.gene054804 | MS.gene48509 | 0.812018 | 5.24E-51 | -1.69E-46 |
| MS.gene054955 | MS.gene48509 | 0.849478 | 3.15E-60 | -1.69E-46 |
| MS.gene054983 | MS.gene48509 | 0.830017 | 3.72E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene48509 | 0.829479 | 5.03E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene48509 | 0.884454 | 1.89E-71 | -1.69E-46 |
| MS.gene055206 | MS.gene48509 | 0.803113 | 4.07E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene48509 | 0.862316 | 5.53E-64 | -1.69E-46 |
| MS.gene055390 | MS.gene48509 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene055412 | MS.gene48509 | 0.800262 | 1.57E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene48509 | 0.857229 | 1.88E-62 | -1.69E-46 |
| MS.gene055428 | MS.gene48509 | 0.85561 | 5.62E-62 | -1.69E-46 |
| MS.gene05553 | MS.gene48509 | 0.83399 | 3.88E-56 | -1.69E-46 |
| MS.gene055604 | MS.gene48509 | 0.804224 | 2.39E-49 | -1.69E-46 |
| MS.gene055666 | MS.gene48509 | 0.802127 | 6.50E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene48509 | 0.814884 | 1.23E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene48509 | 0.831192 | 1.92E-55 | -1.69E-46 |
| MS.gene056191 | MS.gene48509 | 0.85403 | 1.61E-61 | -1.69E-46 |
| MS.gene056314 | MS.gene48509 | 0.805303 | 1.42E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene48509 | 0.864932 | 8.52E-65 | -1.69E-46 |
| MS.gene056410 | MS.gene48509 | 0.854421 | 1.24E-61 | -1.69E-46 |
| MS.gene056511 | MS.gene48509 | 0.845594 | 3.68E-59 | -1.69E-46 |
| MS.gene056609 | MS.gene48509 | 0.84825 | 6.91E-60 | -1.69E-46 |
| MS.gene056612 | MS.gene48509 | 0.842331 | 2.76E-58 | -1.69E-46 |
| MS.gene056659 | MS.gene48509 | 0.816816 | 4.55E-52 | -1.69E-46 |
| MS.gene056719 | MS.gene48509 | 0.828812 | 7.30E-55 | -1.69E-46 |
| MS.gene056796 | MS.gene48509 | 0.837454 | 5.15E-57 | -1.69E-46 |
| MS.gene056899 | MS.gene48509 | -0.813789 | 2.14E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene48509 | 0.877258 | 7.26E-69 | -1.69E-46 |
| MS.gene057123 | MS.gene48509 | 0.861592 | 9.21E-64 | -1.69E-46 |
| MS.gene057124 | MS.gene48509 | 0.800598 | 1.34E-48 | -1.69E-46 |
| MS.gene057129 | MS.gene48509 | 0.801647 | 8.16E-49 | -1.69E-46 |
| MS.gene057146 | MS.gene48509 | 0.843162 | 1.66E-58 | -1.69E-46 |
| MS.gene057295 | MS.gene48509 | 0.822522 | 2.26E-53 | -1.69E-46 |
| MS.gene057330 | MS.gene48509 | 0.816103 | 6.57E-52 | -1.69E-46 |
| MS.gene05733 | MS.gene48509 | 0.836354 | 9.83E-57 | -1.69E-46 |
| MS.gene057416 | MS.gene48509 | 0.809979 | 1.45E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene48509 | 0.823669 | 1.22E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene48509 | 0.805453 | 1.32E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene48509 | 0.824957 | 6.09E-54 | -1.69E-46 |
| MS.gene057749 | MS.gene48509 | 0.859801 | 3.22E-63 | -1.69E-46 |
| MS.gene057796 | MS.gene48509 | 0.818123 | 2.31E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene48509 | 0.854327 | 1.33E-61 | -1.69E-46 |
| MS.gene057825 | MS.gene48509 | 0.800279 | 1.55E-48 | -1.69E-46 |
| MS.gene058002 | MS.gene48509 | 0.852376 | 4.81E-61 | -1.69E-46 |
| MS.gene058006 | MS.gene48509 | 0.813354 | 2.67E-51 | -1.69E-46 |
| MS.gene058007 | MS.gene48509 | 0.826097 | 3.27E-54 | -1.69E-46 |
| MS.gene058019 | MS.gene48509 | 0.830307 | 3.16E-55 | -1.69E-46 |
| MS.gene058082 | MS.gene48509 | 0.832747 | 7.93E-56 | -1.69E-46 |
| MS.gene05811 | MS.gene48509 | 0.865003 | 8.09E-65 | -1.69E-46 |
| MS.gene058142 | MS.gene48509 | 0.808463 | 3.06E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene48509 | 0.845224 | 4.64E-59 | -1.69E-46 |
| MS.gene058276 | MS.gene48509 | 0.818093 | 2.35E-52 | -1.69E-46 |
| MS.gene058293 | MS.gene48509 | 0.864732 | 9.84E-65 | -1.69E-46 |
| MS.gene058365 | MS.gene48509 | 0.814573 | 1.44E-51 | -1.69E-46 |
| MS.gene058376 | MS.gene48509 | 0.816324 | 5.86E-52 | -1.69E-46 |
| MS.gene058444 | MS.gene48509 | 0.82825 | 9.98E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene48509 | 0.87577 | 2.37E-68 | -1.69E-46 |
| MS.gene058700 | MS.gene48509 | 0.835944 | 1.25E-56 | -1.69E-46 |
| MS.gene058787 | MS.gene48509 | 0.837214 | 5.93E-57 | -1.69E-46 |
| MS.gene058845 | MS.gene48509 | 0.815414 | 9.36E-52 | -1.69E-46 |
| MS.gene05886 | MS.gene48509 | 0.818459 | 1.94E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene48509 | 0.85089 | 1.27E-60 | -1.69E-46 |
| MS.gene05907 | MS.gene48509 | 0.89677 | 2.68E-76 | -1.69E-46 |
| MS.gene059141 | MS.gene48509 | 0.804778 | 1.83E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene48509 | 0.817759 | 2.79E-52 | -1.69E-46 |
| MS.gene059294 | MS.gene48509 | 0.848412 | 6.23E-60 | -1.69E-46 |
| MS.gene059326 | MS.gene48509 | 0.821058 | 4.94E-53 | -1.69E-46 |
| MS.gene05934 | MS.gene48509 | 0.804162 | 2.47E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene48509 | 0.830801 | 2.39E-55 | -1.69E-46 |
| MS.gene059420 | MS.gene48509 | 0.814432 | 1.55E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene48509 | 0.838676 | 2.50E-57 | -1.69E-46 |
| MS.gene059536 | MS.gene48509 | 0.840088 | 1.07E-57 | -1.69E-46 |
| MS.gene059597 | MS.gene48509 | 0.894544 | 2.23E-75 | -1.69E-46 |
| MS.gene05959 | MS.gene48509 | 0.831983 | 1.23E-55 | -1.69E-46 |
| MS.gene059676 | MS.gene48509 | 0.877836 | 4.56E-69 | -1.69E-46 |
| MS.gene059688 | MS.gene48509 | 0.888687 | 4.73E-73 | -1.69E-46 |
| MS.gene059692 | MS.gene48509 | 0.89686 | 2.46E-76 | -1.69E-46 |
| MS.gene059695 | MS.gene48509 | 0.899209 | 2.48E-77 | -1.69E-46 |
| MS.gene059708 | MS.gene48509 | 0.802472 | 5.52E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene48509 | 0.810625 | 1.05E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene48509 | 0.833116 | 6.42E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene48509 | 0.863247 | 2.85E-64 | -1.69E-46 |
| MS.gene059894 | MS.gene48509 | 0.86812 | 8.27E-66 | -1.69E-46 |
| MS.gene059902 | MS.gene48509 | 0.804932 | 1.70E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene48509 | 0.827735 | 1.33E-54 | -1.69E-46 |
| MS.gene059974 | MS.gene48509 | 0.810255 | 1.26E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene48509 | 0.860723 | 1.69E-63 | -1.69E-46 |
| MS.gene060403 | MS.gene48509 | 0.873076 | 1.95E-67 | -1.69E-46 |
| MS.gene060407 | MS.gene48509 | 0.814838 | 1.26E-51 | -1.69E-46 |
| MS.gene060496 | MS.gene48509 | 0.801142 | 1.04E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene48509 | 0.856324 | 3.47E-62 | -1.69E-46 |
| MS.gene060818 | MS.gene48509 | 0.823979 | 1.03E-53 | -1.69E-46 |
| MS.gene060837 | MS.gene48509 | 0.822063 | 2.89E-53 | -1.69E-46 |
| MS.gene060986 | MS.gene48509 | 0.800686 | 1.28E-48 | -1.69E-46 |
| MS.gene061041 | MS.gene48509 | 0.839218 | 1.81E-57 | -1.69E-46 |
| MS.gene061049 | MS.gene48509 | 0.889369 | 2.58E-73 | -1.69E-46 |
| MS.gene061051 | MS.gene48509 | -0.847249 | 1.30E-59 | -1.69E-46 |
| MS.gene061154 | MS.gene48509 | 0.828176 | 1.04E-54 | -1.69E-46 |
| MS.gene061159 | MS.gene48509 | 0.800725 | 1.26E-48 | -1.69E-46 |
| MS.gene061200 | MS.gene48509 | 0.815654 | 8.27E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48509.t1 | MTR_3g101740 | 93.210 | 162 | 8 | 1 | 1 | 159 | 57 | 218 | 9.27e-102 | 291 |
| MS.gene48509.t1 | MTR_3g112410 | 37.719 | 114 | 61 | 3 | 42 | 154 | 13 | 117 | 8.21e-15 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48509.t1 | AT1G07700 | 73.913 | 138 | 35 | 1 | 17 | 154 | 68 | 204 | 2.86e-71 | 213 |
| MS.gene48509.t1 | AT1G07700 | 73.913 | 138 | 35 | 1 | 17 | 154 | 68 | 204 | 2.86e-71 | 213 |
| MS.gene48509.t1 | AT1G07700 | 73.913 | 138 | 35 | 1 | 17 | 154 | 68 | 204 | 2.86e-71 | 213 |
| MS.gene48509.t1 | AT1G07700 | 73.913 | 138 | 35 | 1 | 17 | 154 | 81 | 217 | 1.04e-70 | 213 |
| MS.gene48509.t1 | AT5G42980 | 33.628 | 113 | 67 | 2 | 42 | 154 | 12 | 116 | 3.69e-12 | 60.1 |
| MS.gene48509.t1 | AT3G51030 | 36.111 | 108 | 61 | 2 | 42 | 149 | 13 | 112 | 8.21e-12 | 59.3 |
Find 31 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTCTTTGTCTCTGGTT+GGG | 0.298576 | 3.1:-80782782 | None:intergenic |
| AACAATCCTTTCTTTGTCTC+TGG | 0.330128 | 3.1:-80782787 | None:intergenic |
| CTAGATAAGTCTAAAGGAAC+TGG | 0.380813 | 3.1:+80781930 | MS.gene48509:CDS |
| GCAAAGTTGTGCAAGAAATC+TGG | 0.381106 | 3.1:+80782020 | MS.gene48509:CDS |
| TTGCGGAACGACTTAGAATC+AGG | 0.406477 | 3.1:+80782391 | MS.gene48509:CDS |
| AATTCTCTCACACATTCAAC+AGG | 0.416746 | 3.1:-80781879 | None:intergenic |
| TTATGCTTTAGAAAGATAAC+AGG | 0.421854 | 3.1:-80782056 | None:intergenic |
| ATCCTTTCTTTGTCTCTGGT+TGG | 0.431773 | 3.1:-80782783 | None:intergenic |
| TGCAACTTCCACAAGAGGTT+CGG | 0.443956 | 3.1:-80781977 | None:intergenic |
| CTCTTCCACTTCTACAAAGA+TGG | 0.449719 | 3.1:+80782741 | MS.gene48509:CDS |
| GATTTCTTCCGAACCTCTTG+TGG | 0.457067 | 3.1:+80781969 | MS.gene48509:CDS |
| AAGCCTTGTTGACATTCAAC+GGG | 0.478995 | 3.1:-80781819 | None:intergenic |
| TCATCTTCATCATCTTCATC+AGG | 0.483274 | 3.1:-80781843 | None:intergenic |
| CAAGCCTTGTTGACATTCAA+CGG | 0.505128 | 3.1:-80781820 | None:intergenic |
| AAGTTGCAAGTATATTGAGC+AGG | 0.512637 | 3.1:+80781992 | MS.gene48509:CDS |
| CTACAAAGATGGAAAACTGT+TGG | 0.519244 | 3.1:+80782752 | MS.gene48509:CDS |
| AAAATTCTAGATAAGTCTAA+AGG | 0.519884 | 3.1:+80781924 | MS.gene48509:CDS |
| TCTAAGTCGTTCCGCAACCT+CGG | 0.520659 | 3.1:-80782385 | None:intergenic |
| AACTGGATCTCTTGTTGTGG+TGG | 0.529192 | 3.1:+80781947 | MS.gene48509:CDS |
| TGATGAAGAATCCGAGGTTG+CGG | 0.530168 | 3.1:+80782374 | MS.gene48509:CDS |
| TCCCAACCAGAGACAAAGAA+AGG | 0.565898 | 3.1:+80782781 | MS.gene48509:CDS |
| ATACTTGCAACTTCCACAAG+AGG | 0.567703 | 3.1:-80781982 | None:intergenic |
| TGGTACCTATTAATAAAATG+AGG | 0.575324 | 3.1:+80781784 | MS.gene48509:CDS |
| AGTTGCAAGTATATTGAGCA+GGG | 0.585364 | 3.1:+80781993 | MS.gene48509:CDS |
| CTTCAAGAGATGAGTACTTG+AGG | 0.592510 | 3.1:-80782818 | None:intergenic |
| AAGAGATGAGTACTTGAGGA+TGG | 0.618825 | 3.1:-80782814 | None:intergenic |
| TGCGGAACGACTTAGAATCA+GGG | 0.632999 | 3.1:+80782392 | MS.gene48509:CDS |
| TCTTTGTAGAAGTGGAAGAG+AGG | 0.655662 | 3.1:-80782738 | None:intergenic |
| AGGAACTGGATCTCTTGTTG+TGG | 0.658617 | 3.1:+80781944 | MS.gene48509:CDS |
| TATCCCGTTGAATGTCAACA+AGG | 0.662409 | 3.1:+80781816 | MS.gene48509:CDS |
| TGAGTATGATGAAGAATCCG+AGG | 0.729308 | 3.1:+80782368 | MS.gene48509:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTCAATAAGTTGATTT+TGG | - | chr3.1:80782694-80782713 | None:intergenic | 15.0% |
| !! | AAAATTCTAGATAAGTCTAA+AGG | + | chr3.1:80781924-80781943 | MS.gene48509:CDS | 20.0% |
| !! | ATAATACAAATGTGATACAT+TGG | - | chr3.1:80782227-80782246 | None:intergenic | 20.0% |
| !! | TTAGATATTTATAGCATGTA+GGG | - | chr3.1:80782655-80782674 | None:intergenic | 20.0% |
| !!! | TTGTACCTCATTTTATTAAT+AGG | - | chr3.1:80781792-80781811 | None:intergenic | 20.0% |
| ! | ACAAATTAGTTAGTGACTTA+TGG | + | chr3.1:80782140-80782159 | MS.gene48509:intron | 25.0% |
| ! | GTTAGATATTTATAGCATGT+AGG | - | chr3.1:80782656-80782675 | None:intergenic | 25.0% |
| ! | TAATAATCACAAACTAATGC+AGG | + | chr3.1:80782710-80782729 | MS.gene48509:intron | 25.0% |
| ! | TGGTACCTATTAATAAAATG+AGG | + | chr3.1:80781784-80781803 | MS.gene48509:CDS | 25.0% |
| !! | TATGGATTAAAAACTGTTAG+CGG | + | chr3.1:80782158-80782177 | MS.gene48509:intron | 25.0% |
| !!! | TAGACTTATCTAGAATTTTG+AGG | - | chr3.1:80781923-80781942 | None:intergenic | 25.0% |
| !!! | TTATGCTTTAGAAAGATAAC+AGG | - | chr3.1:80782059-80782078 | None:intergenic | 25.0% |
| AAACATGAAGATGATACTGA+TGG | - | chr3.1:80782460-80782479 | None:intergenic | 30.0% | |
| ATCAATTACCTGCAATAAGA+GGG | - | chr3.1:80782349-80782368 | None:intergenic | 30.0% | |
| CTTAGTATGCATTCAATTGA+TGG | + | chr3.1:80782536-80782555 | MS.gene48509:intron | 30.0% | |
| TTAACTGTTCAGTTTGTCTA+TGG | + | chr3.1:80782101-80782120 | MS.gene48509:intron | 30.0% | |
| !! | GATGGATAATGTTGTTTCTT+AGG | + | chr3.1:80782554-80782573 | MS.gene48509:intron | 30.0% |
| AACAATCCTTTCTTTGTCTC+TGG | - | chr3.1:80782790-80782809 | None:intergenic | 35.0% | |
| AAGTTGCAAGTATATTGAGC+AGG | + | chr3.1:80781992-80782011 | MS.gene48509:CDS | 35.0% | |
| AATTCTCTCACACATTCAAC+AGG | - | chr3.1:80781882-80781901 | None:intergenic | 35.0% | |
| AGTTGCAAGTATATTGAGCA+GGG | + | chr3.1:80781993-80782012 | MS.gene48509:CDS | 35.0% | |
| CATCAATTACCTGCAATAAG+AGG | - | chr3.1:80782350-80782369 | None:intergenic | 35.0% | |
| CTACAAAGATGGAAAACTGT+TGG | + | chr3.1:80782752-80782771 | MS.gene48509:CDS | 35.0% | |
| CTAGATAAGTCTAAAGGAAC+TGG | + | chr3.1:80781930-80781949 | MS.gene48509:CDS | 35.0% | |
| TCATCTTCATCATCTTCATC+AGG | - | chr3.1:80781846-80781865 | None:intergenic | 35.0% | |
| ! | GTTTTCCATCTTTGTAGAAG+TGG | - | chr3.1:80782749-80782768 | None:intergenic | 35.0% |
| AAGAGATGAGTACTTGAGGA+TGG | - | chr3.1:80782817-80782836 | None:intergenic | 40.0% | |
| ATACTTGCAACTTCCACAAG+AGG | - | chr3.1:80781985-80782004 | None:intergenic | 40.0% | |
| ATCCTTTCTTTGTCTCTGGT+TGG | - | chr3.1:80782786-80782805 | None:intergenic | 40.0% | |
| CTCTTCCACTTCTACAAAGA+TGG | + | chr3.1:80782741-80782760 | MS.gene48509:CDS | 40.0% | |
| CTGTTCAGTTTGTCTATGGT+TGG | + | chr3.1:80782105-80782124 | MS.gene48509:intron | 40.0% | |
| CTTCAAGAGATGAGTACTTG+AGG | - | chr3.1:80782821-80782840 | None:intergenic | 40.0% | |
| GCAAAGTTGTGCAAGAAATC+TGG | + | chr3.1:80782020-80782039 | MS.gene48509:CDS | 40.0% | |
| TATCACTTCCCTCTTATTGC+AGG | + | chr3.1:80782338-80782357 | MS.gene48509:intron | 40.0% | |
| TATCCCGTTGAATGTCAACA+AGG | + | chr3.1:80781816-80781835 | MS.gene48509:CDS | 40.0% | |
| TCCTTTCTTTGTCTCTGGTT+GGG | - | chr3.1:80782785-80782804 | None:intergenic | 40.0% | |
| TCTTTGTAGAAGTGGAAGAG+AGG | - | chr3.1:80782741-80782760 | None:intergenic | 40.0% | |
| TGAGTATGATGAAGAATCCG+AGG | + | chr3.1:80782368-80782387 | MS.gene48509:CDS | 40.0% | |
| !! | AAGCCTTGTTGACATTCAAC+GGG | - | chr3.1:80781822-80781841 | None:intergenic | 40.0% |
| !! | CAAGCCTTGTTGACATTCAA+CGG | - | chr3.1:80781823-80781842 | None:intergenic | 40.0% |
| AGGAACTGGATCTCTTGTTG+TGG | + | chr3.1:80781944-80781963 | MS.gene48509:CDS | 45.0% | |
| GATTTCTTCCGAACCTCTTG+TGG | + | chr3.1:80781969-80781988 | MS.gene48509:CDS | 45.0% | |
| TCCCAACCAGAGACAAAGAA+AGG | + | chr3.1:80782781-80782800 | MS.gene48509:CDS | 45.0% | |
| TGATGAAGAATCCGAGGTTG+CGG | + | chr3.1:80782374-80782393 | MS.gene48509:CDS | 45.0% | |
| TGCAACTTCCACAAGAGGTT+CGG | - | chr3.1:80781980-80781999 | None:intergenic | 45.0% | |
| TGCGGAACGACTTAGAATCA+GGG | + | chr3.1:80782392-80782411 | MS.gene48509:CDS | 45.0% | |
| TTGCGGAACGACTTAGAATC+AGG | + | chr3.1:80782391-80782410 | MS.gene48509:CDS | 45.0% | |
| ! | AACTGGATCTCTTGTTGTGG+TGG | + | chr3.1:80781947-80781966 | MS.gene48509:CDS | 45.0% |
| TCTAAGTCGTTCCGCAACCT+CGG | - | chr3.1:80782388-80782407 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 80781783 | 80782860 | 80781783 | ID=MS.gene48509 |
| chr3.1 | mRNA | 80781783 | 80782860 | 80781783 | ID=MS.gene48509.t1;Parent=MS.gene48509 |
| chr3.1 | exon | 80781783 | 80782079 | 80781783 | ID=MS.gene48509.t1.exon1;Parent=MS.gene48509.t1 |
| chr3.1 | CDS | 80781783 | 80782079 | 80781783 | ID=cds.MS.gene48509.t1;Parent=MS.gene48509.t1 |
| chr3.1 | exon | 80782360 | 80782413 | 80782360 | ID=MS.gene48509.t1.exon2;Parent=MS.gene48509.t1 |
| chr3.1 | CDS | 80782360 | 80782413 | 80782360 | ID=cds.MS.gene48509.t1;Parent=MS.gene48509.t1 |
| chr3.1 | exon | 80782732 | 80782860 | 80782732 | ID=MS.gene48509.t1.exon3;Parent=MS.gene48509.t1 |
| chr3.1 | CDS | 80782732 | 80782860 | 80782732 | ID=cds.MS.gene48509.t1;Parent=MS.gene48509.t1 |
| Gene Sequence |
| Protein sequence |
