AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67919

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67919.t1 MTR_3g107160 40.614 293 142 9 7 292 63 330 4.61e-51 173
MS.gene67919.t1 MTR_3g107120 42.403 283 131 9 11 288 79 334 9.84e-49 167
MS.gene67919.t1 MTR_7g034090 66.142 127 37 2 173 299 147 267 4.59e-48 166
MS.gene67919.t1 MTR_7g034090 56.391 133 48 3 4 136 60 182 2.61e-33 127
MS.gene67919.t1 MTR_3g107100 42.215 289 143 8 5 291 63 329 1.49e-47 164
MS.gene67919.t1 MTR_2g007450 38.387 310 152 11 10 294 73 368 2.70e-46 161
MS.gene67919.t1 MTR_1g053025 37.037 297 156 10 10 294 60 337 6.44e-38 138
MS.gene67919.t1 MTR_2g007400 34.069 317 162 12 3 298 62 352 2.23e-33 127
MS.gene67919.t1 MTR_3g026920 34.505 313 139 12 10 290 76 354 4.65e-33 125
MS.gene67919.t1 MTR_2g007500 34.686 271 155 7 27 288 68 325 3.82e-30 116
MS.gene67919.t1 MTR_3g108150 35.315 286 156 10 11 292 68 328 2.34e-29 115
MS.gene67919.t1 MTR_2g005990 29.766 299 181 7 10 297 63 343 3.48e-29 115
MS.gene67919.t1 MTR_4g098590 33.564 289 164 10 9 288 55 324 7.71e-29 114
MS.gene67919.t1 MTR_3g107140 47.771 157 69 5 4 159 52 196 1.28e-28 109
MS.gene67919.t1 MTR_7g034120 32.753 287 154 12 19 298 1 255 6.40e-28 110
MS.gene67919.t1 MTR_7g088260 32.746 284 164 9 11 292 146 404 1.02e-27 112
MS.gene67919.t1 MTR_7g075820 31.169 308 175 10 3 295 56 341 1.60e-27 110
MS.gene67919.t1 MTR_7g032370 32.414 290 147 11 15 298 49 295 2.38e-27 109
MS.gene67919.t1 MTR_5g083890 31.613 310 177 10 4 295 57 349 5.63e-27 110
MS.gene67919.t1 MTR_7g083740 31.164 292 146 9 4 290 68 309 7.19e-27 108
MS.gene67919.t1 MTR_3g099830 31.788 302 156 9 4 297 63 322 1.93e-26 107
MS.gene67919.t1 MTR_7g007080 32.872 289 163 8 11 292 62 326 6.32e-26 106
MS.gene67919.t1 MTR_1g107385 32.787 305 169 11 7 295 86 370 5.16e-25 104
MS.gene67919.t1 MTR_4g039440 32.313 294 171 9 4 292 80 350 1.09e-24 103
MS.gene67919.t1 MTR_2g008320 31.741 293 167 9 4 290 103 368 1.43e-23 100
MS.gene67919.t1 MTR_6g045333 30.877 285 164 10 17 292 127 387 1.60e-23 100
MS.gene67919.t1 MTR_1g041245 31.615 291 170 11 11 297 66 331 3.05e-23 98.6
MS.gene67919.t1 MTR_7g056433 31.250 288 171 8 6 290 65 328 6.10e-23 97.8
MS.gene67919.t1 MTR_2g008310 31.973 294 160 10 4 290 53 313 1.14e-22 97.4
MS.gene67919.t1 MTR_7g098480 32.740 281 155 9 15 290 77 328 5.57e-22 95.9
MS.gene67919.t1 MTR_7g098570 31.488 289 158 9 11 290 75 332 8.03e-22 95.5
MS.gene67919.t1 MTR_7g055943 32.069 290 168 11 7 293 94 357 1.15e-21 94.7
MS.gene67919.t1 MTR_3g040700 32.423 293 151 12 4 288 91 344 2.75e-21 93.6
MS.gene67919.t1 MTR_6g022500 31.707 287 162 11 10 292 161 417 9.91e-21 92.4
MS.gene67919.t1 MTR_7g098510 31.228 285 156 10 15 290 77 330 1.02e-20 91.7
MS.gene67919.t1 MTR_5g073420 32.632 285 155 13 10 292 69 318 1.83e-20 90.9
MS.gene67919.t1 MTR_7g007340 33.937 221 116 7 81 297 111 305 2.17e-20 90.5
MS.gene67919.t1 MTR_7g033990 29.577 284 164 11 15 291 70 324 2.44e-20 90.9
MS.gene67919.t1 MTR_0492s0020 30.526 285 167 9 10 294 140 393 2.68e-20 90.9
MS.gene67919.t1 MTR_7g033915 31.164 292 139 15 11 291 66 306 3.18e-20 90.1
MS.gene67919.t1 MTR_0543s0010 30.526 285 167 9 10 294 140 393 3.97e-20 90.5
MS.gene67919.t1 MTR_6g011460 32.660 297 170 11 4 298 97 365 5.24e-20 90.1
MS.gene67919.t1 MTR_5g058000 33.106 293 156 12 4 292 59 315 1.53e-19 88.2
MS.gene67919.t1 MTR_3g036250 31.293 294 154 12 4 288 53 307 1.76e-19 87.8
MS.gene67919.t1 MTR_3g071850 29.932 294 175 11 7 292 80 350 1.86e-19 89.4
MS.gene67919.t1 MTR_6g040270 31.359 287 159 14 11 292 66 319 2.05e-19 87.8
MS.gene67919.t1 MTR_2g007360 30.545 275 141 13 42 298 79 321 2.32e-19 87.8
MS.gene67919.t1 MTR_7g115270 29.861 288 177 7 11 288 67 339 2.55e-19 87.8
MS.gene67919.t1 MTR_1g085120 32.526 289 161 13 10 292 152 412 2.81e-19 88.2
MS.gene67919.t1 MTR_1g085120 32.526 289 161 13 10 292 227 487 4.12e-19 87.8
MS.gene67919.t1 MTR_5g007410 30.488 246 146 7 52 292 124 349 7.49e-19 86.7
MS.gene67919.t1 MTR_5g058100 30.827 266 157 9 28 292 85 324 1.40e-18 85.5
MS.gene67919.t1 MTR_7g056280 32.941 170 101 4 135 299 367 528 2.59e-18 85.5
MS.gene67919.t1 MTR_8g085430 40.000 145 71 6 13 154 76 207 1.55e-17 82.0
MS.gene67919.t1 MTR_7g034115 29.392 296 159 14 15 297 70 328 2.15e-17 82.0
MS.gene67919.t1 MTR_3g108560 30.208 288 171 8 9 294 73 332 3.41e-17 81.6
MS.gene67919.t1 MTR_5g073340 28.807 243 145 8 52 287 79 300 1.00e-16 79.3
MS.gene67919.t1 MTR_1089s0010 30.545 275 160 9 18 292 74 317 2.72e-16 79.0
MS.gene67919.t1 MTR_7g075800 29.180 305 158 14 31 294 82 369 2.81e-16 79.7
MS.gene67919.t1 MTR_1g066830 32.472 271 152 10 27 292 83 327 3.48e-16 78.6
MS.gene67919.t1 MTR_1g085120 32.411 253 143 11 10 256 239 469 3.86e-16 79.0
MS.gene67919.t1 MTR_7g026500 30.000 250 140 10 52 290 111 336 7.79e-16 77.8
MS.gene67919.t1 MTR_5g045170 29.918 244 146 8 51 290 108 330 1.04e-15 77.4
MS.gene67919.t1 MTR_1g108820 28.146 302 167 11 4 290 59 325 2.58e-15 75.9
MS.gene67919.t1 MTR_2g036470 27.551 294 183 10 4 290 65 335 3.32e-15 75.9
MS.gene67919.t1 MTR_3g108560 30.400 250 148 7 9 256 73 298 4.67e-15 74.7
MS.gene67919.t1 MTR_0009s0080 31.959 291 163 14 10 292 78 341 6.39e-15 75.1
MS.gene67919.t1 MTR_7g114710 27.778 288 157 11 6 288 63 304 7.49e-15 74.7
MS.gene67919.t1 MTR_3g109982 33.784 222 127 7 74 294 216 418 1.18e-14 74.7
MS.gene67919.t1 MTR_3g109982 36.879 141 76 4 9 149 66 193 1.50e-11 65.5
MS.gene67919.t1 MTR_3g106960 36.709 158 83 6 141 293 1 146 1.23e-14 72.4
MS.gene67919.t1 MTR_3g087260 32.129 249 130 10 56 294 181 400 2.29e-14 73.6
MS.gene67919.t1 MTR_6g022360 31.250 240 141 9 12 249 93 310 3.47e-14 72.8
MS.gene67919.t1 MTR_5g073370 29.018 224 134 6 77 291 114 321 3.48e-14 72.8
MS.gene67919.t1 MTR_2g007090 31.443 194 94 6 109 298 85 243 5.00e-14 71.6
MS.gene67919.t1 MTR_7g056417 34.568 162 82 5 74 224 3 151 1.42e-13 67.8
MS.gene67919.t1 MTR_5g045510 29.412 289 150 12 10 290 69 311 1.77e-13 70.5
MS.gene67919.t1 MTR_5g013500 27.241 290 168 9 11 292 50 304 1.84e-13 70.5
MS.gene67919.t1 MTR_5g061680 32.780 241 114 10 11 249 70 264 2.39e-13 69.7
MS.gene67919.t1 MTR_7g056287 30.169 295 154 13 9 292 60 313 3.97e-13 69.3
MS.gene67919.t1 MTR_5g031970 27.372 274 142 9 26 292 74 297 7.24e-13 68.6
MS.gene67919.t1 MTR_7g056277 29.831 295 155 13 9 292 316 569 9.01e-13 68.9
MS.gene67919.t1 MTR_7g056020 32.093 215 119 7 82 292 124 315 1.06e-12 68.9
MS.gene67919.t1 MTR_3g105060 26.990 289 170 10 3 288 64 314 1.17e-12 68.2
MS.gene67919.t1 MTR_7g007370 32.584 178 100 4 78 255 94 251 2.48e-12 67.0
MS.gene67919.t1 MTR_6g029350 31.928 166 91 6 134 297 1 146 5.05e-12 65.1
MS.gene67919.t1 MTR_5g063950 29.286 280 166 13 18 292 51 303 8.82e-12 65.5
MS.gene67919.t1 MTR_6g022520 30.741 270 156 10 28 292 11 254 1.46e-11 64.7
MS.gene67919.t1 MTR_3g110010 36.879 141 76 4 9 149 66 193 1.49e-11 65.5
MS.gene67919.t1 MTR_3g082890 27.961 304 177 10 5 288 143 424 2.69e-11 64.3
MS.gene67919.t1 MTR_2g007550 27.907 215 121 8 94 292 89 285 4.60e-11 63.2
MS.gene67919.t1 MTR_1g108820 28.358 268 150 9 4 256 59 299 5.49e-11 63.2
MS.gene67919.t1 MTR_5g076150 33.333 192 91 8 82 251 26 202 6.02e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCATCTTGAAAATATT+TGG 0.116376 7.2:+63674033 MS.gene67919:CDS
CTCGTGGTTCTCAAGCTTTC+GGG 0.195457 7.2:+63673942 MS.gene67919:CDS
CGGAGTCGTTAAAGCGAAAA+AGG 0.212517 7.2:-63673701 None:intergenic
CTACTCAACATAAGCCTAAA+AGG 0.260287 7.2:+63674756 MS.gene67919:CDS
GAGATTGCATGTTAATTATA+AGG 0.268124 7.2:+63674069 MS.gene67919:CDS
CCTCGTGGTTCTCAAGCTTT+CGG 0.271967 7.2:+63673941 MS.gene67919:CDS
CTCAAGACGGAAAGTCTTAA+TGG 0.275190 7.2:-63673760 None:intergenic
GCGAATAAATCCTCAAGAAT+TGG 0.290792 7.2:-63674098 None:intergenic
TCGTCTGATGTTTCTTCGTC+AGG 0.302944 7.2:-63674660 None:intergenic
AGATTGCATGTTAATTATAA+GGG 0.354855 7.2:+63674070 MS.gene67919:CDS
GGTAATTATTGATGACGAAC+AGG 0.396813 7.2:+63674268 MS.gene67919:CDS
CGAATAAATCCTCAAGAATT+GGG 0.404622 7.2:-63674097 None:intergenic
TCAAAAGTTTGACGTTGATC+AGG 0.426123 7.2:+63674533 MS.gene67919:CDS
TAATGGAAAGTGCGGGATCT+CGG 0.430913 7.2:-63673743 None:intergenic
AATGGAAAGTGCGGGATCTC+GGG 0.465859 7.2:-63673742 None:intergenic
TGGGTCAAATTATGAAAAGT+AGG 0.478446 7.2:-63674423 None:intergenic
GAACTGCTTTCAACAGAAAA+CGG 0.485067 7.2:-63674223 None:intergenic
CGTCTGATGTTTCTTCGTCA+GGG 0.486062 7.2:-63674659 None:intergenic
CATTAAGACTTTCCGTCTTG+AGG 0.487991 7.2:+63673761 MS.gene67919:CDS
GCTCCTTTACCTCATAGTTG+AGG 0.512824 7.2:+63675988 MS.gene67919:CDS
GGTATTATCTCCGTCGTGGT+TGG 0.523330 7.2:+63674472 MS.gene67919:CDS
ATACCTCAACTATGAGGTAA+AGG 0.526664 7.2:-63675991 None:intergenic
CCATTTGAAAACACTGTGAC+TGG 0.529490 7.2:-63673814 None:intergenic
CTATAGACACATCGAGATGA+AGG 0.534929 7.2:-63673869 None:intergenic
ATATGTACAGTCTCTGCGCT+TGG 0.551267 7.2:+63674247 MS.gene67919:CDS
AAGGGGATGCCCAATTCTTG+AGG 0.558181 7.2:+63674088 MS.gene67919:CDS
CTGTGACTGGAGCGGTTATG+TGG 0.560103 7.2:-63673801 None:intergenic
TCTGCAATTGAGAATACTGA+TGG 0.574711 7.2:-63673916 None:intergenic
TTATGCATACCTCAACTATG+AGG 0.581230 7.2:-63675997 None:intergenic
AAGAGATTCGTAGGAATGAA+CGG 0.594481 7.2:-63674132 None:intergenic
ATGTTCGAAGGTGTAATGAA+AGG 0.597357 7.2:-63674728 None:intergenic
GATTGCATGTTAATTATAAG+GGG 0.598378 7.2:+63674071 MS.gene67919:CDS
AAAGTCTTAATGGAAAGTGC+GGG 0.600226 7.2:-63673750 None:intergenic
TTCAGAGGTAAGAGATTCGT+AGG 0.607995 7.2:-63674141 None:intergenic
GTGGTTCTCAAGCTTTCGGG+TGG 0.608403 7.2:+63673945 MS.gene67919:CDS
GATTGGTATTATCTCCGTCG+TGG 0.608620 7.2:+63674468 MS.gene67919:CDS
AATCTCTTACCTCTGAAGAG+TGG 0.619548 7.2:+63674147 MS.gene67919:CDS
GGACAGTAAAACAGAGTAGA+CGG 0.623203 7.2:-63673721 None:intergenic
TGAAAACACTGTGACTGGAG+CGG 0.630934 7.2:-63673809 None:intergenic
GAAAGTCTTAATGGAAAGTG+CGG 0.631534 7.2:-63673751 None:intergenic
CTGCAATTGAGAATACTGAT+GGG 0.648768 7.2:-63673915 None:intergenic
AATTGTACACAACCTCAAGA+CGG 0.654069 7.2:-63673773 None:intergenic
TGAGTAGATTGCATGTTCGA+AGG 0.654459 7.2:-63674740 None:intergenic
TCTCAATTGCAGAACCCTCG+TGG 0.671036 7.2:+63673926 MS.gene67919:CDS
GTCAACTTTGTGCTACAACG+TGG 0.676402 7.2:+63673837 MS.gene67919:CDS
CTAAGAATTGCCAACCACGA+CGG 0.685740 7.2:-63674482 None:intergenic
CGAAGATTATGTGATAGTCG+CGG 0.693691 7.2:+63674689 MS.gene67919:CDS
CGAAAGCTTGAGAACCACGA+GGG 0.721360 7.2:-63673940 None:intergenic
CCGAAAGCTTGAGAACCACG+AGG 0.738779 7.2:-63673941 None:intergenic
AGGTGTAATGAAAGGCAATG+CGG 0.741413 7.2:-63674720 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAAAAAATAAATAGTG+AGG + chr7.2:63675538-63675557 MS.gene67919:intron 10.0%
!! TTTACTTAAAGTTAAATTAT+TGG - chr7.2:63675504-63675523 None:intergenic 10.0%
!!! CGTTTTAAAAAAAAAATAAA+AGG + chr7.2:63675239-63675258 MS.gene67919:intron 10.0%
!! AAAAATTAAAAGTCACAATA+AGG - chr7.2:63675481-63675500 None:intergenic 15.0%
!! AAGTCTTTACAAATAAAAAT+AGG - chr7.2:63675056-63675075 None:intergenic 15.0%
!! TCAATATAAAAGAATAAAGA+AGG - chr7.2:63675662-63675681 None:intergenic 15.0%
!!! AATAGGTATATGTTTAATTT+CGG - chr7.2:63675922-63675941 None:intergenic 15.0%
!! AATAAAAGGTAGCAAAATTA+TGG + chr7.2:63675253-63675272 MS.gene67919:intron 20.0%
!! ACCATAAACTATTAGAAATA+AGG - chr7.2:63674365-63674384 None:intergenic 20.0%
!! AGATTGCATGTTAATTATAA+GGG + chr7.2:63674070-63674089 MS.gene67919:CDS 20.0%
!! ATGAATATTTAATTCGTGTA+CGG - chr7.2:63675382-63675401 None:intergenic 20.0%
!!! ACCTTATTTCTAATAGTTTA+TGG + chr7.2:63674361-63674380 MS.gene67919:intron 20.0%
!!! GATAATAGACTTTTTATTTC+AGG - chr7.2:63674858-63674877 None:intergenic 20.0%
! AAAAATAGGAAAGCAATTAC+TGG - chr7.2:63675042-63675061 None:intergenic 25.0%
! AAACAACTAATATTGTGAGA+CGG + chr7.2:63675628-63675647 MS.gene67919:intron 25.0%
! AAACGTCAATATTTAAGAGA+CGG - chr7.2:63675224-63675243 None:intergenic 25.0%
! CTCACAATATTAGTTGTTTA+AGG - chr7.2:63675627-63675646 None:intergenic 25.0%
! CTCTTCATCTTGAAAATATT+TGG + chr7.2:63674033-63674052 MS.gene67919:CDS 25.0%
! GAGATTGCATGTTAATTATA+AGG + chr7.2:63674069-63674088 MS.gene67919:CDS 25.0%
! GATTGCATGTTAATTATAAG+GGG + chr7.2:63674071-63674090 MS.gene67919:CDS 25.0%
! GCATAGAACTACTAATAAAA+TGG - chr7.2:63675094-63675113 None:intergenic 25.0%
! GTTAAAGACTATAATATGAC+AGG - chr7.2:63675837-63675856 None:intergenic 25.0%
! GTTGAAAATGACAATGTTAA+AGG - chr7.2:63675750-63675769 None:intergenic 25.0%
! TATTCTCTCTATGAAAGAAT+TGG + chr7.2:63675421-63675440 MS.gene67919:intron 25.0%
!!! ACTAATAAAATGGATTAACG+TGG - chr7.2:63675084-63675103 None:intergenic 25.0%
!!! CTAATAAAATGGATTAACGT+GGG - chr7.2:63675083-63675102 None:intergenic 25.0%
!!! TGCTTCTTTTTTTCTTTGAT+TGG + chr7.2:63674451-63674470 MS.gene67919:CDS 25.0%
AAGTTAAGTAAACTAGAGGA+AGG - chr7.2:63674945-63674964 None:intergenic 30.0%
ACAGATAAATAAGGATTCCT+TGG - chr7.2:63675714-63675733 None:intergenic 30.0%
ACTGTCACTATACAAGTAAT+AGG - chr7.2:63675939-63675958 None:intergenic 30.0%
ATAAGTAGTTATGTTCTCCA+AGG + chr7.2:63675694-63675713 MS.gene67919:intron 30.0%
CATAGAGAGAATAAGATTAG+TGG - chr7.2:63675414-63675433 None:intergenic 30.0%
CGAATAAATCCTCAAGAATT+GGG - chr7.2:63674100-63674119 None:intergenic 30.0%
GTAGAAGTTAAGTAAACTAG+AGG - chr7.2:63674949-63674968 None:intergenic 30.0%
TACGACTAGACAGATAAATA+AGG - chr7.2:63675723-63675742 None:intergenic 30.0%
TGGGTCAAATTATGAAAAGT+AGG - chr7.2:63674426-63674445 None:intergenic 30.0%
! TAACGACATATTTCTATGAC+AGG + chr7.2:63675958-63675977 MS.gene67919:intron 30.0%
! TTGAAAACAGGTTTGGTATT+TGG - chr7.2:63675787-63675806 None:intergenic 30.0%
! TTTATGGTTCTGCATATTCT+AGG + chr7.2:63674377-63674396 MS.gene67919:intron 30.0%
!! AGAAAAAAAGAAGCACTAGT+TGG - chr7.2:63674446-63674465 None:intergenic 30.0%
!! GAAAAAAAGAAGCACTAGTT+GGG - chr7.2:63674445-63674464 None:intergenic 30.0%
!!! AAGATGAAGAGTTTTAAGAG+AGG - chr7.2:63674024-63674043 None:intergenic 30.0%
!!! AGATGAAGAGTTTTAAGAGA+GGG - chr7.2:63674023-63674042 None:intergenic 30.0%
AACTAATATTGTGAGACGGA+GGG + chr7.2:63675632-63675651 MS.gene67919:intron 35.0%
AAGAGATTCGTAGGAATGAA+CGG - chr7.2:63674135-63674154 None:intergenic 35.0%
AATTCGTGTACGGTGTAAAA+AGG - chr7.2:63675372-63675391 None:intergenic 35.0%
AATTGTACACAACCTCAAGA+CGG - chr7.2:63673776-63673795 None:intergenic 35.0%
AGCAATTACTGGATCATTCA+AGG - chr7.2:63675031-63675050 None:intergenic 35.0%
ATGCATAAGCATAGAGTATG+TGG + chr7.2:63675128-63675147 MS.gene67919:intron 35.0%
ATTCGTGTACGGTGTAAAAA+GGG - chr7.2:63675371-63675390 None:intergenic 35.0%
CTACTCAACATAAGCCTAAA+AGG + chr7.2:63674756-63674775 MS.gene67919:CDS 35.0%
CTGCAATTGAGAATACTGAT+GGG - chr7.2:63673918-63673937 None:intergenic 35.0%
GAAAGTCTTAATGGAAAGTG+CGG - chr7.2:63673754-63673773 None:intergenic 35.0%
GAACTGCTTTCAACAGAAAA+CGG - chr7.2:63674226-63674245 None:intergenic 35.0%
GCGAATAAATCCTCAAGAAT+TGG - chr7.2:63674101-63674120 None:intergenic 35.0%
GTCAATATTTAAGAGACGGA+GGG - chr7.2:63675220-63675239 None:intergenic 35.0%
GTTGGAATTGAAAACAGGTT+TGG - chr7.2:63675794-63675813 None:intergenic 35.0%
TCAAAAGTTTGACGTTGATC+AGG + chr7.2:63674533-63674552 MS.gene67919:CDS 35.0%
TCTCTATGAAAGAATTGGCT+GGG + chr7.2:63675426-63675445 MS.gene67919:intron 35.0%
TCTGCAATTGAGAATACTGA+TGG - chr7.2:63673919-63673938 None:intergenic 35.0%
TGCATAAGCATAGAGTATGT+GGG + chr7.2:63675129-63675148 MS.gene67919:intron 35.0%
! AAAGTCTTAATGGAAAGTGC+GGG - chr7.2:63673753-63673772 None:intergenic 35.0%
! ATAGAAGTACAACAAGGTGT+TGG - chr7.2:63675880-63675899 None:intergenic 35.0%
! ATGTTCGAAGGTGTAATGAA+AGG - chr7.2:63674731-63674750 None:intergenic 35.0%
! CAGTCACAGTGTTTTCAAAT+GGG + chr7.2:63673815-63673834 MS.gene67919:CDS 35.0%
! TAAGGCATCAATTACAAAGC+TGG - chr7.2:63675463-63675482 None:intergenic 35.0%
! TAGAGGATAGAAGTACAACA+AGG - chr7.2:63675886-63675905 None:intergenic 35.0%
! TATGTTTAATTTCGGACGCT+TGG - chr7.2:63675914-63675933 None:intergenic 35.0%
! TGGTATTTGGTGACATTGTT+CGG - chr7.2:63675774-63675793 None:intergenic 35.0%
! TTTGAATGGTGCTTTTACTG+TGG + chr7.2:63673971-63673990 MS.gene67919:CDS 35.0%
!! GGTAATTATTGATGACGAAC+AGG + chr7.2:63674268-63674287 MS.gene67919:CDS 35.0%
ACAGGCTCAAAACTCATAGT+CGG - chr7.2:63675819-63675838 None:intergenic 40.0%
AGGTGTAATGAAAGGCAATG+CGG - chr7.2:63674723-63674742 None:intergenic 40.0%
CAAAACTCATAGTCGGATGT+TGG - chr7.2:63675812-63675831 None:intergenic 40.0%
CAACTAATATTGTGAGACGG+AGG + chr7.2:63675631-63675650 MS.gene67919:intron 40.0%
CATTAAGACTTTCCGTCTTG+AGG + chr7.2:63673761-63673780 MS.gene67919:CDS 40.0%
CCATTTGAAAACACTGTGAC+TGG - chr7.2:63673817-63673836 None:intergenic 40.0%
CGAAGATTATGTGATAGTCG+CGG + chr7.2:63674689-63674708 MS.gene67919:CDS 40.0%
CGGATGTTGGAATTGAAAAC+AGG - chr7.2:63675799-63675818 None:intergenic 40.0%
CGTCAATATTTAAGAGACGG+AGG - chr7.2:63675221-63675240 None:intergenic 40.0%
CTATAGACACATCGAGATGA+AGG - chr7.2:63673872-63673891 None:intergenic 40.0%
CTCAAGACGGAAAGTCTTAA+TGG - chr7.2:63673763-63673782 None:intergenic 40.0%
CTCTCTATGAAAGAATTGGC+TGG + chr7.2:63675425-63675444 MS.gene67919:intron 40.0%
GGACAGTAAAACAGAGTAGA+CGG - chr7.2:63673724-63673743 None:intergenic 40.0%
GGAGTCGTTAAAGCGAAAAA+GGG - chr7.2:63673703-63673722 None:intergenic 40.0%
TACCATGCAACTGCTAGATT+TGG + chr7.2:63674966-63674985 MS.gene67919:intron 40.0%
TCTATTATCCTCATGCCCAA+GGG + chr7.2:63674869-63674888 MS.gene67919:intron 40.0%
TGAGTAGATTGCATGTTCGA+AGG - chr7.2:63674743-63674762 None:intergenic 40.0%
TTCAGAGGTAAGAGATTCGT+AGG - chr7.2:63674144-63674163 None:intergenic 40.0%
TTCGTGTACGGTGTAAAAAG+GGG - chr7.2:63675370-63675389 None:intergenic 40.0%
! AAAGCTTTTCCACTCTTCAG+AGG - chr7.2:63674159-63674178 None:intergenic 40.0%
! AATCTCTTACCTCTGAAGAG+TGG + chr7.2:63674147-63674166 MS.gene67919:CDS 40.0%
! ATGGTTCTGCATATTCTAGG+TGG + chr7.2:63674380-63674399 MS.gene67919:intron 40.0%
! CCAGTCACAGTGTTTTCAAA+TGG + chr7.2:63673814-63673833 MS.gene67919:CDS 40.0%
! CTAGCGTAAGCTTACCTTTT+AGG - chr7.2:63674773-63674792 None:intergenic 40.0%
! GTCACTTTGTGATTTCGATG+TGG + chr7.2:63675275-63675294 MS.gene67919:intron 40.0%
AAGGTAAGCTTACGCTAGCA+AGG + chr7.2:63674775-63674794 MS.gene67919:intron 45.0%
ACCATGACAATCTCGAACAG+AGG + chr7.2:63674828-63674847 MS.gene67919:intron 45.0%
ATATGTACAGTCTCTGCGCT+TGG + chr7.2:63674247-63674266 MS.gene67919:CDS 45.0%
CACCAAATCTAGCAGTTGCA+TGG - chr7.2:63674971-63674990 None:intergenic 45.0%
CCATGACAATCTCGAACAGA+GGG + chr7.2:63674829-63674848 MS.gene67919:intron 45.0%
CGGAGTCGTTAAAGCGAAAA+AGG - chr7.2:63673704-63673723 None:intergenic 45.0%
CGTCTGATGTTTCTTCGTCA+GGG - chr7.2:63674662-63674681 None:intergenic 45.0%
CTAAGAATTGCCAACCACGA+CGG - chr7.2:63674485-63674504 None:intergenic 45.0%
GATTGGTATTATCTCCGTCG+TGG + chr7.2:63674468-63674487 MS.gene67919:CDS 45.0%
GTCAACTTTGTGCTACAACG+TGG + chr7.2:63673837-63673856 MS.gene67919:CDS 45.0%
GTCTATTATCCTCATGCCCA+AGG + chr7.2:63674868-63674887 MS.gene67919:intron 45.0%
TCGTCTGATGTTTCTTCGTC+AGG - chr7.2:63674663-63674682 None:intergenic 45.0%
TGAAAACACTGTGACTGGAG+CGG - chr7.2:63673812-63673831 None:intergenic 45.0%
TTGGACGCAGGATGTATATC+TGG - chr7.2:63675861-63675880 None:intergenic 45.0%
! GGTGCTTTTACTGTGGAAGT+GGG + chr7.2:63673978-63673997 MS.gene67919:CDS 45.0%
! GTGCTTTTACTGTGGAAGTG+GGG + chr7.2:63673979-63673998 MS.gene67919:CDS 45.0%
! TAATGGAAAGTGCGGGATCT+CGG - chr7.2:63673746-63673765 None:intergenic 45.0%
! TGGTGCTTTTACTGTGGAAG+TGG + chr7.2:63673977-63673996 MS.gene67919:CDS 45.0%
!! CTTTCGGGTGGTGTTTTGAA+TGG + chr7.2:63673957-63673976 MS.gene67919:CDS 45.0%
AAGGGGATGCCCAATTCTTG+AGG + chr7.2:63674088-63674107 MS.gene67919:CDS 50.0%
CCCTCTGTTCGAGATTGTCA+TGG - chr7.2:63674832-63674851 None:intergenic 50.0%
CCTCGTGGTTCTCAAGCTTT+CGG + chr7.2:63673941-63673960 MS.gene67919:CDS 50.0%
CGAAAGCTTGAGAACCACGA+GGG - chr7.2:63673943-63673962 None:intergenic 50.0%
CGTAAGTTTACACGCTGCAG+AGG - chr7.2:63674895-63674914 None:intergenic 50.0%
CTCGTGGTTCTCAAGCTTTC+GGG + chr7.2:63673942-63673961 MS.gene67919:CDS 50.0%
GGTATTATCTCCGTCGTGGT+TGG + chr7.2:63674472-63674491 MS.gene67919:CDS 50.0%
TCTCAATTGCAGAACCCTCG+TGG + chr7.2:63673926-63673945 MS.gene67919:CDS 50.0%
! AATGGAAAGTGCGGGATCTC+GGG - chr7.2:63673745-63673764 None:intergenic 50.0%
! ACGTGCAGTTTTGCTGGTAG+AGG - chr7.2:63675346-63675365 None:intergenic 50.0%
! TAAGAGACGGAGGGAGTACA+TGG - chr7.2:63675211-63675230 None:intergenic 50.0%
! TCGGACGCTTGGTAACATAG+AGG - chr7.2:63675903-63675922 None:intergenic 50.0%
! TGCAGTTTTGCTGGTAGAGG+AGG - chr7.2:63675343-63675362 None:intergenic 50.0%
!! AGGAGGAGTAGAAGGCATCA+GGG - chr7.2:63675326-63675345 None:intergenic 50.0%
!! TACAACAAGGTGTTGGACGC+AGG - chr7.2:63675873-63675892 None:intergenic 50.0%
CCGAAAGCTTGAGAACCACG+AGG - chr7.2:63673944-63673963 None:intergenic 55.0%
CTGTGACTGGAGCGGTTATG+TGG - chr7.2:63673804-63673823 None:intergenic 55.0%
GCTGGTAGAGGAGGAGTAGA+AGG - chr7.2:63675334-63675353 None:intergenic 55.0%
GTGGTTCTCAAGCTTTCGGG+TGG + chr7.2:63673945-63673964 MS.gene67919:CDS 55.0%
! AGGGGAACGTGCAGTTTTGC+TGG - chr7.2:63675352-63675371 None:intergenic 55.0%
!! GAGGAGGAGTAGAAGGCATC+AGG - chr7.2:63675327-63675346 None:intergenic 55.0%
!! GGAGGAGTAGAAGGCATCAG+GGG - chr7.2:63675325-63675344 None:intergenic 55.0%
TACACGCTGCAGAGGCCCTT+GGG - chr7.2:63674887-63674906 None:intergenic 60.0%
TTACACGCTGCAGAGGCCCT+TGG - chr7.2:63674888-63674907 None:intergenic 60.0%
!! GAAGGCATCAGGGGCAAAGC+TGG - chr7.2:63675316-63675335 None:intergenic 60.0%
TGCAGAGGCCCTTGGGCATG+AGG - chr7.2:63674880-63674899 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 63673681 63676008 63673681 ID=MS.gene67919
chr7.2 mRNA 63673681 63676008 63673681 ID=MS.gene67919.t1;Parent=MS.gene67919
chr7.2 exon 63673681 63674289 63673681 ID=MS.gene67919.t1.exon1;Parent=MS.gene67919.t1
chr7.2 CDS 63673681 63674289 63673681 ID=cds.MS.gene67919.t1;Parent=MS.gene67919.t1
chr7.2 exon 63674399 63674554 63674399 ID=MS.gene67919.t1.exon2;Parent=MS.gene67919.t1
chr7.2 CDS 63674399 63674554 63674399 ID=cds.MS.gene67919.t1;Parent=MS.gene67919.t1
chr7.2 exon 63674654 63674777 63674654 ID=MS.gene67919.t1.exon3;Parent=MS.gene67919.t1
chr7.2 CDS 63674654 63674777 63674654 ID=cds.MS.gene67919.t1;Parent=MS.gene67919.t1
chr7.2 exon 63675980 63676008 63675980 ID=MS.gene67919.t1.exon4;Parent=MS.gene67919.t1
chr7.2 CDS 63675980 63676008 63675980 ID=cds.MS.gene67919.t1;Parent=MS.gene67919.t1
Gene Sequence

>MS.gene67919

ATGGGGGCAGACGAAAATACCCTTTTTCGCTTTAACGACTCCGTCTACTCTGTTTTACTGTCCCGAGATCCCGCACTTTCCATTAAGACTTTCCGTCTTGAGGTTGTGTACAATTTTAATCCACATAACCGCTCCAGTCACAGTGTTTTCAAATGGGTCAACTTTGTGCTACAACGTGGAGTTGAATACCTTCATCTCGATGTGTCTATAGTGACTTGTAGTCAACTCTGTTTACCCATCAGTATTCTCAATTGCAGAACCCTCGTGGTTCTCAAGCTTTCGGGTGGTGTTTTGAATGGTGCTTTTACTGTGGAAGTGGGGTTTTCTTCTGTTGTACTACCCTCTCTTAAAACTCTTCATCTTGAAAATATTTGGTTCTCAAAAGTTCGAGATTGCATGTTAATTATAAGGGGATGCCCAATTCTTGAGGATTTATTCGCATCTGATTTGTCCGTTCATTCCTACGAATCTCTTACCTCTGAAGAGTGGAAAAGCTTTTGTTTAAGTAACTTGACTCGAGCTGATATTGATTACACTTGTTGCCGTTTTCTGTTGAAAGCAGTTCAATATGTACAGTCTCTGCGCTTGGTAATTATTGATGACGAACAGGTTTGTTTATGACTTTCTTACTTACTGTAGAATGCAAAAAAAAAAAAAAAGTGATTGATATGCAACTTTTGACCTTATTTCTAATAGTTTATGGTTCTGCATATTCTAGGTGGACAACTGTATTGATTCAATTCCTACTTTTCATAATTTGACCCAACTAGTGCTTCTTTTTTTCTTTGATTGGTATTATCTCCGTCGTGGTTGGCAATTCTTAGTAGAACTTCTCAATCACTGCTCTAGACTTCAAAAGTTTGACGTTGATCAGGTTTGTTAAAGATCATATCTCACTTATCTTTTCACATCACACTCTTAGAGTTTGAGTTTTTTATTTTATTTTATTTTATTTTGTTTGCGTTATCAACAGATCGTCCCTGACGAAGAAACATCAGACGAAGATGACGAAGATTATGTGATAGTCGCGGATAATGTTCCGCATTGCCTTTCATTACACCTTCGAACATGCAATCTACTCAACATAAGCCTAAAAGGTAAGCTTACGCTAGCAAGGTATATTTTGAAGAATGCAAGAGTTTTACAAACCATGACAATCTCGAACAGAGGGCAGCCTGAAATAAAAAGTCTATTATCCTCATGCCCAAGGGCCTCTGCAGCGTGTAAACTTACGTTTCAATGTAAGTAGCATTTACTCTATCCTTCCTCTAGTTTACTTAACTTCTACCATGCAACTGCTAGATTTGGTGCTTTGAATTAGAATTTGATGTCATTAAATTTGTATATCCTTGAATGATCCAGTAATTGCTTTCCTATTTTTATTTGTAAAGACTTTATACCCACGTTAATCCATTTTATTAGTAGTTCTATGCTAAATTCTAACTTGATGCATAAGCATAGAGTATGTGGTTGATGCATAAGCATAGAGTATGTGGGTGACAATTGTAAATTAGTGTCATATGTTTGCCATGTACTCCCTCCGTCTCTTAAATATTGACGTTTTAAAAAAAAAATAAAAGGTAGCAAAATTATGGTCACTTTGTGATTTCGATGTGGAATTAAATAAGTTTTCCAGCTTTGCCCCTGATGCCTTCTACTCCTCCTCTACCAGCAAAACTGCACGTTCCCCTTTTTACACCGTACACGAATTAAATATTCATGTTACTGATCCACTAATCTTATTCTCTCTATGAAAGAATTGGCTGGGCTGCATTTATACCAGCTTTGTAATTGATGCCTTATTGTGACTTTTAATTTTTCCAATAATTTAACTTTAAGTAAATGTGACTTTAAATAATATTAAAAAATAAATAGTGAGGTTATTTTAGTAAAATTAGTATGTATGTTGACTAATTTATTACATTTCTTAATTTGTGTGTAAACCTTAAACAACTAATATTGTGAGACGGAGGGAGTACCTTCTTTATTCTTTTATATTGAAACATGTATTAAATAAGTAGTTATGTTCTCCAAGGAATCCTTATTTATCTGTCTAGTCGTATTCTCCTTTAACATTGTCATTTTCAACACCGAACAATGTCACCAAATACCAAACCTGTTTTCAATTCCAACATCCGACTATGAGTTTTGAGCCTGTCATATTATAGTCTTTAACTCCAGATATACATCCTGCGTCCAACACCTTGTTGTACTTCTATCCTCTATGTTACCAAGCGTCCGAAATTAAACATATACCTATTACTTGTATAGTGACAGTAACGACATATTTCTATGACAGGACATGTTGCTCCTTTACCTCATAGTTGA
Protein sequence

>MS.gene67919.t1

MGADENTLFRFNDSVYSVLLSRDPALSIKTFRLEVVYNFNPHNRSSHSVFKWVNFVLQRGVEYLHLDVSIVTCSQLCLPISILNCRTLVVLKLSGGVLNGAFTVEVGFSSVVLPSLKTLHLENIWFSKVRDCMLIIRGCPILEDLFASDLSVHSYESLTSEEWKSFCLSNLTRADIDYTCCRFLLKAVQYVQSLRLVIIDDEQVDNCIDSIPTFHNLTQLVLLFFFDWYYLRRGWQFLVELLNHCSRLQKFDVDQIVPDEETSDEDDEDYVIVADNVPHCLSLHLRTCNLLNISLKGHVAPLPHS