AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67920.t1 MTR_7g034090 74.737 95 18 2 4 98 60 148 2.26e-36 131
MS.gene67920.t1 MTR_3g107160 40.722 194 100 6 9 195 65 250 1.35e-31 118
MS.gene67920.t1 MTR_1g053025 43.684 190 95 8 1 182 52 237 4.54e-31 117
MS.gene67920.t1 MTR_3g107140 50.336 149 61 6 4 145 52 194 1.41e-29 108
MS.gene67920.t1 MTR_3g107120 42.328 189 96 6 11 195 79 258 1.73e-29 112
MS.gene67920.t1 MTR_3g107100 40.306 196 104 5 5 195 63 250 1.78e-28 110
MS.gene67920.t1 MTR_2g007450 41.848 184 82 7 7 170 70 248 4.93e-28 109
MS.gene67920.t1 MTR_2g007400 38.350 206 111 7 3 195 62 264 1.40e-26 105
MS.gene67920.t1 MTR_5g058000 38.542 192 96 6 4 188 59 235 2.22e-21 90.5
MS.gene67920.t1 MTR_4g039440 34.146 205 113 6 4 196 80 274 7.51e-20 86.7
MS.gene67920.t1 MTR_1g066830 36.464 181 106 4 10 189 65 237 9.23e-20 85.9
MS.gene67920.t1 MTR_4g098590 34.343 198 113 6 6 191 52 244 5.11e-19 84.0
MS.gene67920.t1 MTR_5g058100 34.807 181 107 5 10 188 66 237 1.52e-18 82.8
MS.gene67920.t1 MTR_2g007500 37.500 160 86 5 28 177 69 224 3.56e-18 81.3
MS.gene67920.t1 MTR_7g055943 33.152 184 110 5 10 189 97 271 1.68e-17 80.1
MS.gene67920.t1 MTR_3g108150 35.829 187 100 5 11 192 68 239 2.32e-17 79.3
MS.gene67920.t1 MTR_7g098480 35.354 198 104 7 5 192 63 246 2.37e-17 79.7
MS.gene67920.t1 MTR_6g011460 35.294 187 111 5 4 189 97 274 2.70e-17 79.3
MS.gene67920.t1 MTR_1g041245 32.632 190 106 6 11 192 66 241 5.82e-17 78.2
MS.gene67920.t1 MTR_5g061680 36.957 184 103 7 5 183 63 238 1.27e-16 76.6
MS.gene67920.t1 MTR_5g045510 35.542 166 84 5 38 188 78 235 5.69e-16 75.5
MS.gene67920.t1 MTR_7g098570 33.171 205 99 8 2 191 67 248 6.12e-16 75.5
MS.gene67920.t1 MTR_6g022360 33.880 183 108 5 11 189 88 261 8.34e-16 75.1
MS.gene67920.t1 MTR_3g026920 34.615 208 91 8 10 191 76 264 1.42e-15 74.3
MS.gene67920.t1 MTR_7g098510 32.632 190 103 6 11 191 73 246 1.47e-15 74.3
MS.gene67920.t1 MTR_1g107385 35.071 211 117 8 1 195 81 287 1.66e-15 74.3
MS.gene67920.t1 MTR_2g008310 36.170 188 107 5 10 192 59 238 1.94e-15 73.9
MS.gene67920.t1 MTR_2g008320 35.829 187 113 5 10 192 109 292 2.80e-15 73.6
MS.gene67920.t1 MTR_7g075820 31.019 216 116 7 3 194 56 262 3.73e-15 73.2
MS.gene67920.t1 MTR_1089s0010 33.929 168 98 4 24 188 77 234 5.48e-15 72.4
MS.gene67920.t1 MTR_1g085120 32.292 192 115 6 4 189 143 325 1.10e-14 72.0
MS.gene67920.t1 MTR_1g085120 32.292 192 115 6 4 189 230 412 1.21e-14 71.6
MS.gene67920.t1 MTR_1g085120 32.292 192 115 6 4 189 218 400 1.30e-14 71.6
MS.gene67920.t1 MTR_5g083890 33.333 204 114 7 11 195 64 264 1.48e-14 71.6
MS.gene67920.t1 MTR_5g073420 32.620 187 106 5 10 192 69 239 1.90e-14 70.9
MS.gene67920.t1 MTR_2g005990 31.884 207 118 7 2 194 58 255 3.20e-14 70.5
MS.gene67920.t1 MTR_3g099830 32.632 190 109 6 13 194 55 233 8.80e-14 68.9
MS.gene67920.t1 MTR_7g083740 34.302 172 77 6 4 170 68 208 1.68e-13 68.2
MS.gene67920.t1 MTR_3g023630 36.429 140 79 4 11 145 63 197 1.69e-13 68.2
MS.gene67920.t1 MTR_0009s0080 34.973 183 102 7 11 188 76 246 2.15e-13 68.2
MS.gene67920.t1 MTR_6g045333 35.359 181 104 6 11 188 121 291 3.02e-13 67.8
MS.gene67920.t1 MTR_7g026500 34.637 179 77 8 27 188 89 244 4.77e-13 67.0
MS.gene67920.t1 MTR_3g095600 31.492 181 91 6 11 189 64 213 6.84e-13 66.2
MS.gene67920.t1 MTR_5g063950 34.356 163 96 6 18 177 51 205 1.16e-12 65.9
MS.gene67920.t1 MTR_5g045170 35.570 149 84 5 53 197 108 248 2.33e-12 65.1
MS.gene67920.t1 MTR_6g022500 33.333 180 93 6 10 187 161 315 4.35e-12 64.3
MS.gene67920.t1 MTR_3g071850 34.118 170 101 5 7 173 80 241 6.95e-12 63.9
MS.gene67920.t1 MTR_0492s0020 33.846 130 76 3 10 138 140 260 8.16e-12 63.5
MS.gene67920.t1 MTR_0543s0010 33.846 130 76 3 10 138 140 260 8.59e-12 63.5
MS.gene67920.t1 MTR_7g032370 34.815 135 73 5 11 138 45 171 1.18e-11 62.8
MS.gene67920.t1 MTR_6g040270 31.694 183 108 6 11 188 66 236 1.85e-11 62.4
MS.gene67920.t1 MTR_7g007340 37.795 127 63 4 72 192 105 221 1.87e-11 62.4
MS.gene67920.t1 MTR_2g007360 37.589 141 73 4 63 195 96 229 2.44e-11 62.0
MS.gene67920.t1 MTR_5g065100 42.446 139 62 8 5 138 63 188 2.81e-11 62.0
MS.gene67920.t1 MTR_6g044572 28.910 211 123 4 3 188 52 260 5.00e-11 61.2
MS.gene67920.t1 MTR_5g031970 28.643 199 124 8 2 192 51 239 5.36e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTGGTTCTCAAGCTTTC+GGG 0.183311 7.2:+63686323 MS.gene67920:CDS
CGAAGTCGTTAAAGCGAAAA+TGG 0.205922 7.2:-63686091 None:intergenic
CTCGAGACCGAAAGTCTTAA+TGG 0.233367 7.2:-63686150 None:intergenic
TCTTGTGGTTCTCAAGCTTT+CGG 0.250286 7.2:+63686322 MS.gene67920:CDS
ATCATAAAATCTTCAAGTTT+AGG 0.321402 7.2:-63686422 None:intergenic
TAGCACAAAGTTGATCCATT+TGG 0.366168 7.2:-63686225 None:intergenic
ACGAAATATGTTTCAAATGA+AGG 0.374457 7.2:-63686397 None:intergenic
CATTTGGAAACACTGCTACT+AGG 0.380786 7.2:-63686209 None:intergenic
GCACTTCCCATTAAGACTTT+CGG 0.381047 7.2:+63686143 MS.gene67920:CDS
AGCTCTTCCACTCATCAGTT+AGG 0.383785 7.2:-63686526 None:intergenic
TAATGGGAAGTGCGGGATCT+CGG 0.428894 7.2:-63686133 None:intergenic
GTATGTAAATCCTCAAGATT+AGG 0.457752 7.2:-63686461 None:intergenic
TTCGGGTTTCAGTGTGGAAG+AGG 0.458055 7.2:+63686340 MS.gene67920:CDS
AATGGGAAGTGCGGGATCTC+GGG 0.465859 7.2:-63686132 None:intergenic
ATTTGGAAACACTGCTACTA+GGG 0.466141 7.2:-63686208 None:intergenic
GCTCTTCCACTCATCAGTTA+GGG 0.472351 7.2:-63686525 None:intergenic
TCGAGACCGAAAGTCTTAAT+GGG 0.475554 7.2:-63686149 None:intergenic
TCTGCGCTTCGAAGTTAAAG+AGG 0.484054 7.2:+63686631 MS.gene67920:CDS
CGAAATATGTTTCAAATGAA+GGG 0.487054 7.2:-63686396 None:intergenic
CTAGTAGCAGTGTTTCCAAA+TGG 0.509551 7.2:+63686210 MS.gene67920:CDS
ATCAGTTAGGGATTCACTAT+CGG 0.521244 7.2:-63686513 None:intergenic
CTGTAGACACATCGAGATGA+AGG 0.536516 7.2:-63686265 None:intergenic
GAAGTCGTTAAAGCGAAAAT+GGG 0.537032 7.2:-63686090 None:intergenic
TCTCAATTGCACAACTCTTG+TGG 0.543991 7.2:+63686307 MS.gene67920:CDS
CATTAAGACTTTCGGTCTCG+AGG 0.551047 7.2:+63686151 MS.gene67920:CDS
CTAGGGTCGTGGCGGTCACG+TGG 0.551986 7.2:-63686191 None:intergenic
CAACGGAAAATAAGCAGGAA+AGG 0.556148 7.2:-63686585 None:intergenic
AAAGTCTTAATGGGAAGTGC+GGG 0.563841 7.2:-63686140 None:intergenic
AAGGGGATGTCCTAATCTTG+AGG 0.570972 7.2:+63686451 MS.gene67920:CDS
TCGGGTTTCAGTGTGGAAGA+GGG 0.577992 7.2:+63686341 MS.gene67920:CDS
AAATGAAGGGTTCTGAGAGA+CGG 0.583159 7.2:-63686383 None:intergenic
ACGTTCAACGGAAAATAAGC+AGG 0.599490 7.2:-63686590 None:intergenic
CAAGCTTTCGGGTTTCAGTG+TGG 0.609670 7.2:+63686334 MS.gene67920:CDS
AAACACTGCTACTAGGGTCG+TGG 0.614792 7.2:-63686202 None:intergenic
ACATTGTGAACTACGTTCAA+CGG 0.625688 7.2:-63686602 None:intergenic
GTGAATCCCTAACTGATGAG+TGG 0.626051 7.2:+63686519 MS.gene67920:CDS
GAAAGTCTTAATGGGAAGTG+CGG 0.626919 7.2:-63686141 None:intergenic
TGTGCAATTGAGAATACTGA+TGG 0.637950 7.2:-63686297 None:intergenic
CACTGCTACTAGGGTCGTGG+CGG 0.638397 7.2:-63686199 None:intergenic
ATCAACTTTGTGCTACAACG+TGG 0.676402 7.2:+63686233 MS.gene67920:CDS
GTGCAATTGAGAATACTGAT+GGG 0.737010 7.2:-63686296 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTATGATCTTTCTA+AGG + chr7.2:63686432-63686451 MS.gene67920:CDS 20.0%
!!! AGATTTTATGATCTTTCTAA+GGG + chr7.2:63686433-63686452 MS.gene67920:CDS 20.0%
!!! ATCATAAAATCTTCAAGTTT+AGG - chr7.2:63686425-63686444 None:intergenic 20.0%
! ACGAAATATGTTTCAAATGA+AGG - chr7.2:63686400-63686419 None:intergenic 25.0%
! CGAAATATGTTTCAAATGAA+GGG - chr7.2:63686399-63686418 None:intergenic 25.0%
!! GATTTTATGATCTTTCTAAG+GGG + chr7.2:63686434-63686453 MS.gene67920:CDS 25.0%
GTATGTAAATCCTCAAGATT+AGG - chr7.2:63686464-63686483 None:intergenic 30.0%
ACATTGTGAACTACGTTCAA+CGG - chr7.2:63686605-63686624 None:intergenic 35.0%
ATCAGTTAGGGATTCACTAT+CGG - chr7.2:63686516-63686535 None:intergenic 35.0%
ATTTGGAAACACTGCTACTA+GGG - chr7.2:63686211-63686230 None:intergenic 35.0%
GAAGTCGTTAAAGCGAAAAT+GGG - chr7.2:63686093-63686112 None:intergenic 35.0%
GTGCAATTGAGAATACTGAT+GGG - chr7.2:63686299-63686318 None:intergenic 35.0%
TGTGCAATTGAGAATACTGA+TGG - chr7.2:63686300-63686319 None:intergenic 35.0%
! TAGCACAAAGTTGATCCATT+TGG - chr7.2:63686228-63686247 None:intergenic 35.0%
ACGTTCAACGGAAAATAAGC+AGG - chr7.2:63686593-63686612 None:intergenic 40.0%
ATCAACTTTGTGCTACAACG+TGG + chr7.2:63686233-63686252 MS.gene67920:CDS 40.0%
CAACGGAAAATAAGCAGGAA+AGG - chr7.2:63686588-63686607 None:intergenic 40.0%
CATTTGGAAACACTGCTACT+AGG - chr7.2:63686212-63686231 None:intergenic 40.0%
CGAAGTCGTTAAAGCGAAAA+TGG - chr7.2:63686094-63686113 None:intergenic 40.0%
CTAGTAGCAGTGTTTCCAAA+TGG + chr7.2:63686210-63686229 MS.gene67920:CDS 40.0%
GAAAGTCTTAATGGGAAGTG+CGG - chr7.2:63686144-63686163 None:intergenic 40.0%
GCACTTCCCATTAAGACTTT+CGG + chr7.2:63686143-63686162 MS.gene67920:CDS 40.0%
TCGAGACCGAAAGTCTTAAT+GGG - chr7.2:63686152-63686171 None:intergenic 40.0%
TCTCAATTGCACAACTCTTG+TGG + chr7.2:63686307-63686326 MS.gene67920:CDS 40.0%
TCTTGTGGTTCTCAAGCTTT+CGG + chr7.2:63686322-63686341 MS.gene67920:CDS 40.0%
! AAAGTCTTAATGGGAAGTGC+GGG - chr7.2:63686143-63686162 None:intergenic 40.0%
!! AAATGAAGGGTTCTGAGAGA+CGG - chr7.2:63686386-63686405 None:intergenic 40.0%
AAGGGGATGTCCTAATCTTG+AGG + chr7.2:63686451-63686470 MS.gene67920:CDS 45.0%
AGCTCTTCCACTCATCAGTT+AGG - chr7.2:63686529-63686548 None:intergenic 45.0%
CATTAAGACTTTCGGTCTCG+AGG + chr7.2:63686151-63686170 MS.gene67920:CDS 45.0%
CTCGAGACCGAAAGTCTTAA+TGG - chr7.2:63686153-63686172 None:intergenic 45.0%
CTGTAGACACATCGAGATGA+AGG - chr7.2:63686268-63686287 None:intergenic 45.0%
CTTGTGGTTCTCAAGCTTTC+GGG + chr7.2:63686323-63686342 MS.gene67920:CDS 45.0%
GCTCTTCCACTCATCAGTTA+GGG - chr7.2:63686528-63686547 None:intergenic 45.0%
GTGAATCCCTAACTGATGAG+TGG + chr7.2:63686519-63686538 MS.gene67920:CDS 45.0%
TCTGCGCTTCGAAGTTAAAG+AGG + chr7.2:63686631-63686650 MS.gene67920:CDS 45.0%
AAACACTGCTACTAGGGTCG+TGG - chr7.2:63686205-63686224 None:intergenic 50.0%
TCGGGTTTCAGTGTGGAAGA+GGG + chr7.2:63686341-63686360 MS.gene67920:CDS 50.0%
! TAATGGGAAGTGCGGGATCT+CGG - chr7.2:63686136-63686155 None:intergenic 50.0%
! TTCGGGTTTCAGTGTGGAAG+AGG + chr7.2:63686340-63686359 MS.gene67920:CDS 50.0%
!! CAAGCTTTCGGGTTTCAGTG+TGG + chr7.2:63686334-63686353 MS.gene67920:CDS 50.0%
! AATGGGAAGTGCGGGATCTC+GGG - chr7.2:63686135-63686154 None:intergenic 55.0%
CACTGCTACTAGGGTCGTGG+CGG - chr7.2:63686202-63686221 None:intergenic 60.0%
CTAGGGTCGTGGCGGTCACG+TGG - chr7.2:63686194-63686213 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 63686071 63686664 63686071 ID=MS.gene67920
chr7.2 mRNA 63686071 63686664 63686071 ID=MS.gene67920.t1;Parent=MS.gene67920
chr7.2 exon 63686071 63686664 63686071 ID=MS.gene67920.t1.exon1;Parent=MS.gene67920.t1
chr7.2 CDS 63686071 63686664 63686071 ID=cds.MS.gene67920.t1;Parent=MS.gene67920.t1
Gene Sequence

>MS.gene67920

ATGGGGGCAGACGAAAGCACCCATTTTCGCTTTAACGACTTCGTCTACTCTGTTTTACTGTCCCGAGATCCCGCACTTCCCATTAAGACTTTCGGTCTCGAGGTTGTATACGTTTTTAATCCACGTGACCGCCACGACCCTAGTAGCAGTGTTTCCAAATGGATCAACTTTGTGCTACAACGTGGAGTTGAATACCTTCATCTCGATGTGTCTACAGTGTCTTTACCCATCAGTATTCTCAATTGCACAACTCTTGTGGTTCTCAAGCTTTCGGGTTTCAGTGTGGAAGAGGGTTTTTCTTCTGTTCTACTTCCGTCTCTCAGAACCCTTCATTTGAAACATATTTCGTTACCTAAACTTGAAGATTTTATGATCTTTCTAAGGGGATGTCCTAATCTTGAGGATTTACATACAGTTTTTCTGTCTTATGATTCTAACGAATCCGATAGTGAATCCCTAACTGATGAGTGGAAGAGCTTTTTCTTAAGTAACTTGACGACAGCTGATATTGATTCCTTTCCTGCTTATTTTCCGTTGAACGTAGTTCACAATGTATCTTCTCTGCGCTTCGAAGTTAAAGAGGTTTGTTTATGA
Protein sequence

>MS.gene67920.t1

MGADESTHFRFNDFVYSVLLSRDPALPIKTFGLEVVYVFNPRDRHDPSSSVSKWINFVLQRGVEYLHLDVSTVSLPISILNCTTLVVLKLSGFSVEEGFSSVLLPSLRTLHLKHISLPKLEDFMIFLRGCPNLEDLHTVFLSYDSNESDSESLTDEWKSFFLSNLTTADIDSFPAYFPLNVVHNVSSLRFEVKEVCL