AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0101710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101710 MtrunA17_Chr1g0154111 92.000 50 4 0 2 51 484 533 2.08e-25 97.4
Msa0101710 MtrunA17_Chr1g0154101 75.556 45 11 0 2 46 336 380 5.02e-17 73.6
Msa0101710 MtrunA17_Chr1g0154241 66.667 51 13 1 2 52 173 219 9.50e-14 63.5
Msa0101710 MtrunA17_Chr1g0154251 53.571 56 25 1 1 55 185 240 2.23e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGCTAGAATTAGCTTCTT+TGG 0.160211 1_3:+8362854 None:intergenic
GCATCAGCCATGCATTTCTT+AGG 0.230325 1_3:+8362974 None:intergenic
TCGCTAGAATTAGCTTCTTT+GGG 0.334685 1_3:+8362855 None:intergenic
CGAAAGAACACTTGATTTGA+GGG 0.397450 1_3:-8362835 None:intergenic
CATCAGCCATGCATTTCTTA+GGG 0.401845 1_3:+8362975 None:intergenic
CCTCGCTTCTTAGTCTTGAC+AGG 0.446448 1_3:+8362885 None:intergenic
CAGTTTCCCTTCCAGCCAAC+CGG 0.446590 1_3:-8362929 Msa0101710:CDS
GCGAAAGAACACTTGATTTG+AGG 0.454066 1_3:-8362836 None:intergenic
TTTCTGTTTGTAGTTTCAAA+AGG 0.457908 1_3:+8363028 None:intergenic
GTTTCAAAAGGTACGGCTAA+TGG 0.461542 1_3:+8363040 None:intergenic
CCTTCCAGCCAACCGGCAAA+AGG 0.478631 1_3:-8362922 Msa0101710:CDS
GTTTGTAGTTTCAAAAGGTA+CGG 0.509428 1_3:+8363033 None:intergenic
CTAGAATTAGCTTCTTTGGG+TGG 0.573009 1_3:+8362858 None:intergenic
CTTCTTAGTCTTGACAGGCA+CGG 0.576311 1_3:+8362890 None:intergenic
TGCTTCCCCTAAGAAATGCA+TGG 0.614007 1_3:-8362981 Msa0101710:CDS
ATCAGCCATGCATTTCTTAG+GGG 0.624023 1_3:+8362976 None:intergenic
CCTGTCAAGACTAAGAAGCG+AGG 0.701933 1_3:-8362885 Msa0101710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTCTGTTTGTAGTTTCAAA+AGG + chr1_3:8362856-8362875 None:intergenic 25.0%
GTTTGTAGTTTCAAAAGGTA+CGG + chr1_3:8362851-8362870 None:intergenic 30.0%
! AACAGAAAGACTTTTAATGC+TGG - chr1_3:8362868-8362887 Msa0101710:CDS 30.0%
! TCGCTAGAATTAGCTTCTTT+GGG + chr1_3:8363029-8363048 None:intergenic 35.0%
! TTCGCTAGAATTAGCTTCTT+TGG + chr1_3:8363030-8363049 None:intergenic 35.0%
! GTTTCAAAAGGTACGGCTAA+TGG + chr1_3:8362844-8362863 None:intergenic 40.0%
ATCAGCCATGCATTTCTTAG+GGG + chr1_3:8362908-8362927 None:intergenic 40.0%
CATCAGCCATGCATTTCTTA+GGG + chr1_3:8362909-8362928 None:intergenic 40.0%
! CTAGAATTAGCTTCTTTGGG+TGG + chr1_3:8363026-8363045 None:intergenic 40.0%
TGCTTCCCCTAAGAAATGCA+TGG - chr1_3:8362900-8362919 Msa0101710:CDS 45.0%
GCATCAGCCATGCATTTCTT+AGG + chr1_3:8362910-8362929 None:intergenic 45.0%
! TTCTTAGTCCTTTTGCCGGT+TGG + chr1_3:8362970-8362989 None:intergenic 45.0%
!! CTTCTTAGTCTTGACAGGCA+CGG + chr1_3:8362994-8363013 None:intergenic 45.0%
! CGGCTTCTTAGTCCTTTTGC+CGG + chr1_3:8362974-8362993 None:intergenic 50.0%
!! CCTCGCTTCTTAGTCTTGAC+AGG + chr1_3:8362999-8363018 None:intergenic 50.0%
CCTGTCAAGACTAAGAAGCG+AGG - chr1_3:8362996-8363015 Msa0101710:CDS 50.0%
CAGTTTCCCTTCCAGCCAAC+CGG - chr1_3:8362952-8362971 Msa0101710:CDS 55.0%
!! CTTTTGCCGGTTGGCTGGAA+GGG + chr1_3:8362961-8362980 None:intergenic 55.0%
!! TAGTCCTTTTGCCGGTTGGC+TGG + chr1_3:8362966-8362985 None:intergenic 55.0%
!! CCTTTTGCCGGTTGGCTGGA+AGG + chr1_3:8362962-8362981 None:intergenic 60.0%
CCTTCCAGCCAACCGGCAAA+AGG - chr1_3:8362959-8362978 Msa0101710:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 8362838 8363065 8362838 ID=Msa0101710;Name=Msa0101710
chr1_3 mRNA 8362838 8363065 8362838 ID=Msa0101710-mRNA-1;Parent=Msa0101710;Name=Msa0101710-mRNA-1;_AED=0.49;_eAED=0.63;_QI=0|-1|0|1|-1|1|1|0|75
chr1_3 exon 8362838 8363065 8362838 ID=Msa0101710-mRNA-1:exon:3376;Parent=Msa0101710-mRNA-1
chr1_3 CDS 8362838 8363065 8362838 ID=Msa0101710-mRNA-1:cds;Parent=Msa0101710-mRNA-1
Gene Sequence

>Msa0101710

ATGCCATTAGCCGTACCTTTTGAAACTACAAACAGAAAGACTTTTAATGCTGGTGTTGAATCTGCTTCCCCTAAGAAATGCATGGCTGATGCACTCACTAAGACGACAACTATACAGTTTCCCTTCCAGCCAACCGGCAAAAGGACTAAGAAGCCGTGCCTGTCAAGACTAAGAAGCGAGGAAGACCACCCAAAGAAGCTAATTCTAGCGAAAGAACACTTGATTTGA

Protein sequence

>Msa0101710

MPLAVPFETTNRKTFNAGVESASPKKCMADALTKTTTIQFPFQPTGKRTKKPCLSRLRSEEDHPKKLILAKEHLI*