AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1091710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1091710 MtrunA17_CPg0492821 69.512 82 25 0 1 82 1 82 6.16e-33 109
Msa1091710 MtrunA17_Chr4g0016851 69.512 82 25 0 1 82 1 82 6.16e-33 109
Msa1091710 MtrunA17_Chr4g0015751 69.512 82 25 0 1 82 1 82 6.16e-33 109
Msa1091710 MtrunA17_Chr4g0017011 96.296 54 2 0 1 54 1 54 1.52e-32 108
Msa1091710 MtrunA17_Chr5g0423051 94.444 54 3 0 1 54 1 54 3.59e-31 105
Msa1091710 MtrunA17_Chr4g0016841 61.538 91 21 2 14 104 9 85 3.80e-31 105
Msa1091710 MtrunA17_Chr6g0451331 90.323 31 3 0 24 54 41 71 5.11e-14 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1091710 ATCG00580.1 69.512 82 25 0 1 82 1 82 9.51e-34 112
Msa1091710 ATCG00560.1 97.297 37 1 0 68 104 2 38 3.55e-19 74.3

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 7_3:-7163136 Msa1091710:CDS
GTAGCTCTATAATTCGGATT+AGG 0.338134 7_3:+7162874 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 7_3:+7163170 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 7_3:-7163219 Msa1091710:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 7_3:-7163271 Msa1091710:exon
ATACCCTCCCTATTCATTGC+GGG 0.391873 7_3:-7163178 Msa1091710:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 7_3:+7163171 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.410190 7_3:-7162816 Msa1091710:CDS
CTTTCTGTAAAATACTCGTT+TGG 0.412027 7_3:+7163103 None:intergenic
TGTGTCGTAGCTCTATAATT+CGG 0.415726 7_3:+7162868 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 7_3:+7163213 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 7_3:-7163262 Msa1091710:exon
ATTCGGATTAGGTTTATCGT+TGG 0.512045 7_3:+7162885 None:intergenic
GAATCGTACCAGTCTCTACT+GGG 0.515213 7_3:-7162815 Msa1091710:CDS
TTTGACTGTATAAGAATATG+TGG 0.517465 7_3:-7163287 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 7_3:-7163157 Msa1091710:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 7_3:-7163158 Msa1091710:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 7_3:-7163174 Msa1091710:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 7_3:+7163109 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 7_3:+7162807 None:intergenic
GTATAAGAATATGTGGAGCT+CGG 0.584815 7_3:-7163280 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 7_3:-7163179 Msa1091710:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 7_3:+7163175 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 7_3:+7163174 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.711157 7_3:-7162814 Msa1091710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTAGTAGATCTTTTT+AGG - chr7_3:7163009-7163028 Msa1091710:intron 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG + chr7_3:7163199-7163218 None:intergenic 20.0%
!!! AATTCAACATTTTGTTCATT+TGG + chr7_3:7163200-7163219 None:intergenic 20.0%
! TTATTAATGGAATTCCTTGT+CGG + chr7_3:7162958-7162977 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG - chr7_3:7163012-7163031 Msa1091710:intron 25.0%
! TATTGATCCCAAAAAAAAGA+CGG + chr7_3:7163113-7163132 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG - chr7_3:7162816-7162835 Msa1091710:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + chr7_3:7162825-7162844 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG + chr7_3:7162935-7162954 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG - chr7_3:7162959-7162978 Msa1091710:intron 30.0%
GTTGTTCCAAAGAATCAAAA+CGG + chr7_3:7162985-7163004 None:intergenic 30.0%
AACCATCGTACTGTAAAAAT+GGG + chr7_3:7163064-7163083 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG - chr7_3:7162899-7162918 Msa1091710:intron 35.0%
! TATTTTACAGAAAGCCGACA+AGG - chr7_3:7162941-7162960 Msa1091710:intron 35.0%
TCAAAACGGCCAGTTATTAA+TGG + chr7_3:7162971-7162990 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG + chr7_3:7163059-7163078 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG - chr7_3:7163059-7163078 Msa1091710:intron 35.0%
CAACCATCGTACTGTAAAAA+TGG + chr7_3:7163065-7163084 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG - chr7_3:7163063-7163082 Msa1091710:intron 35.0%
! ATTCGGATTAGGTTTATCGT+TGG + chr7_3:7163153-7163172 None:intergenic 35.0%
! GTAGCTCTATAATTCGGATT+AGG + chr7_3:7163164-7163183 None:intergenic 35.0%
! TGTGTCGTAGCTCTATAATT+CGG + chr7_3:7163170-7163189 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG - chr7_3:7162856-7162875 Msa1091710:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG + chr7_3:7162867-7162886 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + chr7_3:7162868-7162887 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG + chr7_3:7162929-7162948 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG - chr7_3:7162976-7162995 Msa1091710:intron 40.0%
TAGGTCCTATCTATGGTCAT+TGG + chr7_3:7163040-7163059 None:intergenic 40.0%
AATGGGATAGGTCCTATCTA+TGG + chr7_3:7163047-7163066 None:intergenic 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG - chr7_3:7163103-7163122 Msa1091710:intron 40.0%
GATCCCAAAAAAAAGACGGT+GGG + chr7_3:7163109-7163128 None:intergenic 40.0%
TGATCCCAAAAAAAAGACGG+TGG + chr7_3:7163110-7163129 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG + chr7_3:7163231-7163250 None:intergenic 40.0%
ATACCCTCCCTATTCATTGC+GGG - chr7_3:7162857-7162876 Msa1091710:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - chr7_3:7162877-7162896 Msa1091710:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - chr7_3:7162878-7162897 Msa1091710:CDS 45.0%
AGGCTCCAATGACCATAGAT+AGG - chr7_3:7163032-7163051 Msa1091710:intron 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG - chr7_3:7163102-7163121 Msa1091710:intron 45.0%
TGAATCGTACCAGTCTCTAC+TGG - chr7_3:7163219-7163238 Msa1091710:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG - chr7_3:7163220-7163239 Msa1091710:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG - chr7_3:7163221-7163240 Msa1091710:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG + chr7_3:7162863-7162882 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG - chr7_3:7163073-7163092 Msa1091710:intron 50.0%
TATGTGGAGCTCGGCATGTC+TGG - chr7_3:7162764-7162783 Msa1091710:CDS 55.0%
CCAACCCGCAATGAATAGGG+AGG + chr7_3:7162864-7162883 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - chr7_3:7162861-7162880 Msa1091710:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG - chr7_3:7163081-7163100 Msa1091710:intron 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG - chr7_3:7162773-7162792 Msa1091710:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 7162762 7163295 7162762 ID=Msa1091710;Name=Msa1091710
chr7_3 mRNA 7162762 7163295 7162762 ID=Msa1091710-mRNA-1;Parent=Msa1091710;Name=Msa1091710-mRNA-1;_AED=0.22;_eAED=0.22;_QI=17|0|0|1|0|0|2|0|104
chr7_3 exon 7163118 7163295 7163118 ID=Msa1091710-mRNA-1:exon:2255;Parent=Msa1091710-mRNA-1
chr7_3 exon 7162762 7162912 7162762 ID=Msa1091710-mRNA-1:exon:2254;Parent=Msa1091710-mRNA-1
chr7_3 five_prime_UTR 7163279 7163295 7163279 ID=Msa1091710-mRNA-1:five_prime_utr;Parent=Msa1091710-mRNA-1
chr7_3 CDS 7163118 7163278 7163118 ID=Msa1091710-mRNA-1:cds;Parent=Msa1091710-mRNA-1
chr7_3 CDS 7162762 7162912 7162762 ID=Msa1091710-mRNA-1:cds;Parent=Msa1091710-mRNA-1
Gene Sequence

>Msa1091710

ATGTCTGGAAGCACAGGAGAAAGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGATTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAAT

Protein sequence

>Msa1091710

MSGSTGESSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNDSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLLLIFVLAVLFSNYFFN