AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0449910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0449910 MtrunA17_CPg0492821 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0449910 MtrunA17_Chr4g0016851 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0449910 MtrunA17_Chr4g0015751 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0449910 MtrunA17_Chr4g0017011 98.148 54 1 0 1 54 1 54 2.07e-33 111
Msa0449910 MtrunA17_Chr5g0423051 96.296 54 2 0 1 54 1 54 3.52e-32 107
Msa0449910 MtrunA17_Chr4g0016841 61.538 91 21 2 14 104 9 85 4.52e-31 105
Msa0449910 MtrunA17_Chr6g0451331 90.323 31 3 0 24 54 41 71 5.28e-14 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0449910 ATCG00580.1 70.732 82 24 0 1 82 1 82 1.19e-34 114
Msa0449910 ATCG00560.1 97.297 37 1 0 68 104 2 38 3.75e-19 73.9

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 3_3:+34884451 Msa0449910:CDS
GTAGCTCTATAATTCGGATT+AGG 0.338134 3_3:-34884713 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 3_3:-34884417 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 3_3:+34884368 Msa0449910:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 3_3:+34884316 Msa0449910:exon
ATACCCTCCCTATTCATTGC+GGG 0.391873 3_3:+34884409 Msa0449910:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 3_3:-34884416 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.410190 3_3:+34884771 Msa0449910:CDS
CTTTCTGTAAAATACTCGTT+TGG 0.412027 3_3:-34884484 None:intergenic
TGTGTCGTAGCTCTATAATT+CGG 0.415726 3_3:-34884719 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 3_3:-34884374 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 3_3:+34884325 Msa0449910:exon
ATTCGGATTAGGTTTATCGT+TGG 0.512045 3_3:-34884702 None:intergenic
GAATCGTACCAGTCTCTACT+GGG 0.515213 3_3:+34884772 Msa0449910:CDS
TTTGACTGTATAAGAATATG+TGG 0.517465 3_3:+34884300 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 3_3:+34884430 Msa0449910:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 3_3:+34884429 Msa0449910:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 3_3:+34884413 Msa0449910:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 3_3:-34884478 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 3_3:-34884780 None:intergenic
GTATAAGAATATGTGGAGCT+CGG 0.584815 3_3:+34884307 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 3_3:+34884408 Msa0449910:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 3_3:-34884412 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 3_3:-34884413 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.711157 3_3:+34884773 Msa0449910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTAGTAGATCTTTTT+AGG + chr3_3:34884561-34884580 Msa0449910:intron 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG - chr3_3:34884751-34884770 None:intergenic 20.0%
!!! AATTCAACATTTTGTTCATT+TGG - chr3_3:34884752-34884771 None:intergenic 20.0%
! TTATTAATGGAATTCCTTGT+CGG - chr3_3:34884510-34884529 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG + chr3_3:34884564-34884583 Msa0449910:intron 25.0%
! TATTGATCCCAAAAAAAAGA+CGG - chr3_3:34884665-34884684 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG + chr3_3:34884368-34884387 Msa0449910:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG - chr3_3:34884377-34884396 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG - chr3_3:34884487-34884506 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG + chr3_3:34884511-34884530 Msa0449910:intron 30.0%
GTTGTTCCAAAGAATCAAAA+CGG - chr3_3:34884537-34884556 None:intergenic 30.0%
AACCATCGTACTGTAAAAAT+GGG - chr3_3:34884616-34884635 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG + chr3_3:34884451-34884470 Msa0449910:CDS 35.0%
! TATTTTACAGAAAGCCGACA+AGG + chr3_3:34884493-34884512 Msa0449910:intron 35.0%
TCAAAACGGCCAGTTATTAA+TGG - chr3_3:34884523-34884542 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG - chr3_3:34884611-34884630 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG + chr3_3:34884611-34884630 Msa0449910:intron 35.0%
CAACCATCGTACTGTAAAAA+TGG - chr3_3:34884617-34884636 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG + chr3_3:34884615-34884634 Msa0449910:intron 35.0%
! ATTCGGATTAGGTTTATCGT+TGG - chr3_3:34884705-34884724 None:intergenic 35.0%
! GTAGCTCTATAATTCGGATT+AGG - chr3_3:34884716-34884735 None:intergenic 35.0%
! TGTGTCGTAGCTCTATAATT+CGG - chr3_3:34884722-34884741 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG + chr3_3:34884408-34884427 Msa0449910:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG - chr3_3:34884419-34884438 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG - chr3_3:34884420-34884439 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG - chr3_3:34884481-34884500 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG + chr3_3:34884528-34884547 Msa0449910:intron 40.0%
TAGGTCCTATCTATGGTCAT+TGG - chr3_3:34884592-34884611 None:intergenic 40.0%
AATGGGATAGGTCCTATCTA+TGG - chr3_3:34884599-34884618 None:intergenic 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG + chr3_3:34884655-34884674 Msa0449910:intron 40.0%
GATCCCAAAAAAAAGACGGT+GGG - chr3_3:34884661-34884680 None:intergenic 40.0%
TGATCCCAAAAAAAAGACGG+TGG - chr3_3:34884662-34884681 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG - chr3_3:34884783-34884802 None:intergenic 40.0%
ATACCCTCCCTATTCATTGC+GGG + chr3_3:34884409-34884428 Msa0449910:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG + chr3_3:34884429-34884448 Msa0449910:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG + chr3_3:34884430-34884449 Msa0449910:CDS 45.0%
AGGCTCCAATGACCATAGAT+AGG + chr3_3:34884584-34884603 Msa0449910:intron 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG + chr3_3:34884654-34884673 Msa0449910:intron 45.0%
TGAATCGTACCAGTCTCTAC+TGG + chr3_3:34884771-34884790 Msa0449910:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG + chr3_3:34884772-34884791 Msa0449910:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG + chr3_3:34884773-34884792 Msa0449910:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG - chr3_3:34884415-34884434 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG + chr3_3:34884625-34884644 Msa0449910:intron 50.0%
TATGTGGAGCTCGGCATGTC+TGG + chr3_3:34884316-34884335 Msa0449910:exon 55.0%
CCAACCCGCAATGAATAGGG+AGG - chr3_3:34884416-34884435 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG + chr3_3:34884413-34884432 Msa0449910:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG + chr3_3:34884633-34884652 Msa0449910:intron 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG + chr3_3:34884325-34884344 Msa0449910:exon 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 34884314 34884847 34884314 ID=Msa0449910;Name=Msa0449910
chr3_3 mRNA 34884314 34884847 34884314 ID=Msa0449910-mRNA-1;Parent=Msa0449910;Name=Msa0449910-mRNA-1;_AED=0.22;_eAED=0.22;_QI=17|0|0|1|0|0|2|0|104
chr3_3 exon 34884314 34884491 34884314 ID=Msa0449910-mRNA-1:exon:6213;Parent=Msa0449910-mRNA-1
chr3_3 exon 34884697 34884847 34884697 ID=Msa0449910-mRNA-1:exon:6214;Parent=Msa0449910-mRNA-1
chr3_3 five_prime_UTR 34884314 34884330 34884314 ID=Msa0449910-mRNA-1:five_prime_utr;Parent=Msa0449910-mRNA-1
chr3_3 CDS 34884331 34884491 34884331 ID=Msa0449910-mRNA-1:cds;Parent=Msa0449910-mRNA-1
chr3_3 CDS 34884697 34884847 34884697 ID=Msa0449910-mRNA-1:cds;Parent=Msa0449910-mRNA-1
Gene Sequence

>Msa0449910

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGATTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAAT

Protein sequence

>Msa0449910

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNDSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLLLIFVLAVLFSNYFFN