AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0924980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924980 MtrunA17_CPg0492821 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0924980 MtrunA17_Chr4g0016851 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0924980 MtrunA17_Chr4g0015751 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0924980 MtrunA17_Chr4g0017011 98.148 54 1 0 1 54 1 54 2.07e-33 111
Msa0924980 MtrunA17_Chr5g0423051 96.296 54 2 0 1 54 1 54 3.52e-32 107
Msa0924980 MtrunA17_Chr4g0016841 61.538 91 21 2 14 104 9 85 4.52e-31 105
Msa0924980 MtrunA17_Chr6g0451331 90.323 31 3 0 24 54 41 71 5.28e-14 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924980 ATCG00580.1 70.732 82 24 0 1 82 1 82 1.19e-34 114
Msa0924980 ATCG00560.1 97.297 37 1 0 68 104 2 38 3.75e-19 73.9

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 6_2:+67534977 Msa0924980:CDS
GTAGCTCTATAATTCGGATT+AGG 0.338134 6_2:-67535239 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 6_2:-67534943 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 6_2:+67534894 Msa0924980:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 6_2:+67534842 Msa0924980:exon
ATACCCTCCCTATTCATTGC+GGG 0.391873 6_2:+67534935 Msa0924980:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 6_2:-67534942 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.410190 6_2:+67535297 Msa0924980:CDS
CTTTCTGTAAAATACTCGTT+TGG 0.412027 6_2:-67535010 None:intergenic
TGTGTCGTAGCTCTATAATT+CGG 0.415726 6_2:-67535245 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 6_2:-67534900 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 6_2:+67534851 Msa0924980:exon
ATTCGGATTAGGTTTATCGT+TGG 0.512045 6_2:-67535228 None:intergenic
GAATCGTACCAGTCTCTACT+GGG 0.515213 6_2:+67535298 Msa0924980:CDS
TTTGACTGTATAAGAATATG+TGG 0.517465 6_2:+67534826 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 6_2:+67534956 Msa0924980:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 6_2:+67534955 Msa0924980:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 6_2:+67534939 Msa0924980:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 6_2:-67535004 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 6_2:-67535306 None:intergenic
GTATAAGAATATGTGGAGCT+CGG 0.584815 6_2:+67534833 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 6_2:+67534934 Msa0924980:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 6_2:-67534938 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 6_2:-67534939 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.711157 6_2:+67535299 Msa0924980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTAGTAGATCTTTTT+AGG + chr6_2:67535087-67535106 Msa0924980:intron 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG - chr6_2:67535277-67535296 None:intergenic 20.0%
!!! AATTCAACATTTTGTTCATT+TGG - chr6_2:67535278-67535297 None:intergenic 20.0%
! TTATTAATGGAATTCCTTGT+CGG - chr6_2:67535036-67535055 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG + chr6_2:67535090-67535109 Msa0924980:intron 25.0%
! TATTGATCCCAAAAAAAAGA+CGG - chr6_2:67535191-67535210 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG + chr6_2:67534894-67534913 Msa0924980:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG - chr6_2:67534903-67534922 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG - chr6_2:67535013-67535032 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG + chr6_2:67535037-67535056 Msa0924980:intron 30.0%
GTTGTTCCAAAGAATCAAAA+CGG - chr6_2:67535063-67535082 None:intergenic 30.0%
AACCATCGTACTGTAAAAAT+GGG - chr6_2:67535142-67535161 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG + chr6_2:67534977-67534996 Msa0924980:CDS 35.0%
! TATTTTACAGAAAGCCGACA+AGG + chr6_2:67535019-67535038 Msa0924980:intron 35.0%
TCAAAACGGCCAGTTATTAA+TGG - chr6_2:67535049-67535068 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG - chr6_2:67535137-67535156 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG + chr6_2:67535137-67535156 Msa0924980:intron 35.0%
CAACCATCGTACTGTAAAAA+TGG - chr6_2:67535143-67535162 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG + chr6_2:67535141-67535160 Msa0924980:intron 35.0%
! ATTCGGATTAGGTTTATCGT+TGG - chr6_2:67535231-67535250 None:intergenic 35.0%
! GTAGCTCTATAATTCGGATT+AGG - chr6_2:67535242-67535261 None:intergenic 35.0%
! TGTGTCGTAGCTCTATAATT+CGG - chr6_2:67535248-67535267 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG + chr6_2:67534934-67534953 Msa0924980:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG - chr6_2:67534945-67534964 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG - chr6_2:67534946-67534965 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG - chr6_2:67535007-67535026 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG + chr6_2:67535054-67535073 Msa0924980:intron 40.0%
TAGGTCCTATCTATGGTCAT+TGG - chr6_2:67535118-67535137 None:intergenic 40.0%
AATGGGATAGGTCCTATCTA+TGG - chr6_2:67535125-67535144 None:intergenic 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG + chr6_2:67535181-67535200 Msa0924980:intron 40.0%
GATCCCAAAAAAAAGACGGT+GGG - chr6_2:67535187-67535206 None:intergenic 40.0%
TGATCCCAAAAAAAAGACGG+TGG - chr6_2:67535188-67535207 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG - chr6_2:67535309-67535328 None:intergenic 40.0%
ATACCCTCCCTATTCATTGC+GGG + chr6_2:67534935-67534954 Msa0924980:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG + chr6_2:67534955-67534974 Msa0924980:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG + chr6_2:67534956-67534975 Msa0924980:CDS 45.0%
AGGCTCCAATGACCATAGAT+AGG + chr6_2:67535110-67535129 Msa0924980:intron 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG + chr6_2:67535180-67535199 Msa0924980:intron 45.0%
TGAATCGTACCAGTCTCTAC+TGG + chr6_2:67535297-67535316 Msa0924980:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG + chr6_2:67535298-67535317 Msa0924980:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG + chr6_2:67535299-67535318 Msa0924980:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG - chr6_2:67534941-67534960 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG + chr6_2:67535151-67535170 Msa0924980:intron 50.0%
TATGTGGAGCTCGGCATGTC+TGG + chr6_2:67534842-67534861 Msa0924980:exon 55.0%
CCAACCCGCAATGAATAGGG+AGG - chr6_2:67534942-67534961 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG + chr6_2:67534939-67534958 Msa0924980:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG + chr6_2:67535159-67535178 Msa0924980:intron 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG + chr6_2:67534851-67534870 Msa0924980:exon 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 67534840 67535373 67534840 ID=Msa0924980;Name=Msa0924980
chr6_2 mRNA 67534840 67535373 67534840 ID=Msa0924980-mRNA-1;Parent=Msa0924980;Name=Msa0924980-mRNA-1;_AED=0.22;_eAED=0.22;_QI=17|0|0|1|0|0|2|0|104
chr6_2 exon 67534840 67535017 67534840 ID=Msa0924980-mRNA-1:exon:10704;Parent=Msa0924980-mRNA-1
chr6_2 exon 67535223 67535373 67535223 ID=Msa0924980-mRNA-1:exon:10705;Parent=Msa0924980-mRNA-1
chr6_2 five_prime_UTR 67534840 67534856 67534840 ID=Msa0924980-mRNA-1:five_prime_utr;Parent=Msa0924980-mRNA-1
chr6_2 CDS 67534857 67535017 67534857 ID=Msa0924980-mRNA-1:cds;Parent=Msa0924980-mRNA-1
chr6_2 CDS 67535223 67535373 67535223 ID=Msa0924980-mRNA-1:cds;Parent=Msa0924980-mRNA-1
Gene Sequence

>Msa0924980

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGATTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAAT

Protein sequence

>Msa0924980

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNDSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLLLIFVLAVLFSNYFFN