AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0493180


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0493180 MtrunA17_CPg0492821 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0493180 MtrunA17_Chr4g0016851 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0493180 MtrunA17_Chr4g0015751 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0493180 MtrunA17_Chr4g0017011 98.148 54 1 0 1 54 1 54 2.07e-33 111
Msa0493180 MtrunA17_Chr5g0423051 96.296 54 2 0 1 54 1 54 3.52e-32 107
Msa0493180 MtrunA17_Chr4g0016841 61.538 91 21 2 14 104 9 85 4.52e-31 105
Msa0493180 MtrunA17_Chr6g0451331 90.323 31 3 0 24 54 41 71 5.28e-14 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0493180 ATCG00580.1 70.732 82 24 0 1 82 1 82 1.19e-34 114
Msa0493180 ATCG00560.1 97.297 37 1 0 68 104 2 38 3.75e-19 73.9

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 3_4:-17657432 Msa0493180:CDS
GTAGCTCTATAATTCGGATT+AGG 0.338134 3_4:+17657170 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 3_4:+17657466 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 3_4:-17657515 Msa0493180:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 3_4:-17657567 Msa0493180:exon
ATACCCTCCCTATTCATTGC+GGG 0.391873 3_4:-17657474 Msa0493180:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 3_4:+17657467 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.410190 3_4:-17657112 Msa0493180:CDS
CTTTCTGTAAAATACTCGTT+TGG 0.412027 3_4:+17657399 None:intergenic
TGTGTCGTAGCTCTATAATT+CGG 0.415726 3_4:+17657164 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 3_4:+17657509 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 3_4:-17657558 Msa0493180:exon
ATTCGGATTAGGTTTATCGT+TGG 0.512045 3_4:+17657181 None:intergenic
GAATCGTACCAGTCTCTACT+GGG 0.515213 3_4:-17657111 Msa0493180:CDS
TTTGACTGTATAAGAATATG+TGG 0.517465 3_4:-17657583 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 3_4:-17657453 Msa0493180:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 3_4:-17657454 Msa0493180:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 3_4:-17657470 Msa0493180:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 3_4:+17657405 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 3_4:+17657103 None:intergenic
GTATAAGAATATGTGGAGCT+CGG 0.584815 3_4:-17657576 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 3_4:-17657475 Msa0493180:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 3_4:+17657471 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 3_4:+17657470 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.711157 3_4:-17657110 Msa0493180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTAGTAGATCTTTTT+AGG - chr3_4:17657305-17657324 Msa0493180:intron 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG + chr3_4:17657495-17657514 None:intergenic 20.0%
!!! AATTCAACATTTTGTTCATT+TGG + chr3_4:17657496-17657515 None:intergenic 20.0%
! TTATTAATGGAATTCCTTGT+CGG + chr3_4:17657254-17657273 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG - chr3_4:17657308-17657327 Msa0493180:intron 25.0%
! TATTGATCCCAAAAAAAAGA+CGG + chr3_4:17657409-17657428 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG - chr3_4:17657112-17657131 Msa0493180:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + chr3_4:17657121-17657140 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG + chr3_4:17657231-17657250 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG - chr3_4:17657255-17657274 Msa0493180:intron 30.0%
GTTGTTCCAAAGAATCAAAA+CGG + chr3_4:17657281-17657300 None:intergenic 30.0%
AACCATCGTACTGTAAAAAT+GGG + chr3_4:17657360-17657379 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG - chr3_4:17657195-17657214 Msa0493180:intron 35.0%
! TATTTTACAGAAAGCCGACA+AGG - chr3_4:17657237-17657256 Msa0493180:intron 35.0%
TCAAAACGGCCAGTTATTAA+TGG + chr3_4:17657267-17657286 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG + chr3_4:17657355-17657374 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG - chr3_4:17657355-17657374 Msa0493180:intron 35.0%
CAACCATCGTACTGTAAAAA+TGG + chr3_4:17657361-17657380 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG - chr3_4:17657359-17657378 Msa0493180:intron 35.0%
! ATTCGGATTAGGTTTATCGT+TGG + chr3_4:17657449-17657468 None:intergenic 35.0%
! GTAGCTCTATAATTCGGATT+AGG + chr3_4:17657460-17657479 None:intergenic 35.0%
! TGTGTCGTAGCTCTATAATT+CGG + chr3_4:17657466-17657485 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG - chr3_4:17657152-17657171 Msa0493180:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG + chr3_4:17657163-17657182 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + chr3_4:17657164-17657183 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG + chr3_4:17657225-17657244 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG - chr3_4:17657272-17657291 Msa0493180:intron 40.0%
TAGGTCCTATCTATGGTCAT+TGG + chr3_4:17657336-17657355 None:intergenic 40.0%
AATGGGATAGGTCCTATCTA+TGG + chr3_4:17657343-17657362 None:intergenic 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG - chr3_4:17657399-17657418 Msa0493180:intron 40.0%
GATCCCAAAAAAAAGACGGT+GGG + chr3_4:17657405-17657424 None:intergenic 40.0%
TGATCCCAAAAAAAAGACGG+TGG + chr3_4:17657406-17657425 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG + chr3_4:17657527-17657546 None:intergenic 40.0%
ATACCCTCCCTATTCATTGC+GGG - chr3_4:17657153-17657172 Msa0493180:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - chr3_4:17657173-17657192 Msa0493180:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - chr3_4:17657174-17657193 Msa0493180:CDS 45.0%
AGGCTCCAATGACCATAGAT+AGG - chr3_4:17657328-17657347 Msa0493180:intron 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG - chr3_4:17657398-17657417 Msa0493180:intron 45.0%
TGAATCGTACCAGTCTCTAC+TGG - chr3_4:17657515-17657534 Msa0493180:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG - chr3_4:17657516-17657535 Msa0493180:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG - chr3_4:17657517-17657536 Msa0493180:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG + chr3_4:17657159-17657178 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG - chr3_4:17657369-17657388 Msa0493180:intron 50.0%
TATGTGGAGCTCGGCATGTC+TGG - chr3_4:17657060-17657079 Msa0493180:CDS 55.0%
CCAACCCGCAATGAATAGGG+AGG + chr3_4:17657160-17657179 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - chr3_4:17657157-17657176 Msa0493180:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG - chr3_4:17657377-17657396 Msa0493180:intron 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG - chr3_4:17657069-17657088 Msa0493180:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 17657058 17657591 17657058 ID=Msa0493180;Name=Msa0493180
chr3_4 mRNA 17657058 17657591 17657058 ID=Msa0493180-mRNA-1;Parent=Msa0493180;Name=Msa0493180-mRNA-1;_AED=0.22;_eAED=0.22;_QI=17|0|0|1|0|0|2|0|104
chr3_4 exon 17657414 17657591 17657414 ID=Msa0493180-mRNA-1:exon:4947;Parent=Msa0493180-mRNA-1
chr3_4 exon 17657058 17657208 17657058 ID=Msa0493180-mRNA-1:exon:4946;Parent=Msa0493180-mRNA-1
chr3_4 five_prime_UTR 17657575 17657591 17657575 ID=Msa0493180-mRNA-1:five_prime_utr;Parent=Msa0493180-mRNA-1
chr3_4 CDS 17657414 17657574 17657414 ID=Msa0493180-mRNA-1:cds;Parent=Msa0493180-mRNA-1
chr3_4 CDS 17657058 17657208 17657058 ID=Msa0493180-mRNA-1:cds;Parent=Msa0493180-mRNA-1
Gene Sequence

>Msa0493180

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGATTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAAT

Protein sequence

>Msa0493180

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNDSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLLLIFVLAVLFSNYFFN