AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086680 MtrunA17_Chr8g0355631 100.000 37 0 0 1 37 35 71 2.22e-20 74.7
Msa1086680 MtrunA17_Chr4g0015761 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa1086680 MtrunA17_CPg0492811 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa1086680 MtrunA17_Chr4g0016861 100.000 35 0 0 1 35 1 35 9.96e-19 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086680 ATCG00600.1 97.222 36 1 0 1 36 1 36 2.23e-18 69.3

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTGAAGTTTCTCTATT+TGG 0.115333 7_2:+87511902 Msa1086680:CDS
TCATGTCAATTGCCAATTCT+TGG 0.245809 7_2:+87511798 Msa1086680:five_prime_UTR
AATAATCCAGCCAAAGTAAT+AGG 0.315680 7_2:-87511944 None:intergenic
AAACTTCAATCATTTGAGTT+TGG 0.328054 7_2:-87511892 None:intergenic
CTAATTCCTATTACTTTGGC+TGG 0.352028 7_2:+87511938 Msa1086680:CDS
TCTCTATTTGGAATCGTGTT+AGG 0.358426 7_2:+87511914 Msa1086680:CDS
AGGTCTAATTCCTATTACTT+TGG 0.388074 7_2:+87511934 Msa1086680:CDS
ATCATTTGAGTTTGGTTGAA+AGG 0.406278 7_2:-87511884 None:intergenic
GAATCTCAATGACCAAGAAT+TGG 0.414372 7_2:-87511810 None:intergenic
AATTGGCAATTGACATGATA+AGG 0.448909 7_2:-87511793 None:intergenic
TTCTTGGTCATTGAGATTCA+CGG 0.471290 7_2:+87511814 Msa1086680:five_prime_UTR
ATATCGACGTGGTGATCAGT+TGG 0.572503 7_2:+87511985 Msa1086680:CDS
TTTGGCTGGATTATTTGTAA+CGG 0.592623 7_2:+87511952 Msa1086680:CDS
GCATATTTACAATATCGACG+TGG 0.789819 7_2:+87511974 Msa1086680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTCAATCATTTGAGTT+TGG - chr7_2:87511895-87511914 None:intergenic 25.0%
! ATGATTGAAGTTTCTCTATT+TGG + chr7_2:87511902-87511921 Msa1086680:CDS 25.0%
AATTGGCAATTGACATGATA+AGG - chr7_2:87511796-87511815 None:intergenic 30.0%
TCAATACAGATTCCTATTGA+AGG + chr7_2:87511837-87511856 Msa1086680:intron 30.0%
GGTAATATCTATCCTTCAAT+AGG - chr7_2:87511852-87511871 None:intergenic 30.0%
TGGTTGAAAGGAAAAAAAGT+GGG - chr7_2:87511875-87511894 None:intergenic 30.0%
TTGGTTGAAAGGAAAAAAAG+TGG - chr7_2:87511876-87511895 None:intergenic 30.0%
! ATCATTTGAGTTTGGTTGAA+AGG - chr7_2:87511887-87511906 None:intergenic 30.0%
AGGTCTAATTCCTATTACTT+TGG + chr7_2:87511934-87511953 Msa1086680:CDS 30.0%
AATAATCCAGCCAAAGTAAT+AGG - chr7_2:87511947-87511966 None:intergenic 30.0%
TTTGGCTGGATTATTTGTAA+CGG + chr7_2:87511952-87511971 Msa1086680:CDS 30.0%
TCATGTCAATTGCCAATTCT+TGG + chr7_2:87511798-87511817 Msa1086680:five_prime_UTR 35.0%
GAATCTCAATGACCAAGAAT+TGG - chr7_2:87511813-87511832 None:intergenic 35.0%
TTCTTGGTCATTGAGATTCA+CGG + chr7_2:87511814-87511833 Msa1086680:five_prime_UTR 35.0%
GTTGAAAGGAAAAAAAGTGG+GGG - chr7_2:87511873-87511892 None:intergenic 35.0%
GGTTGAAAGGAAAAAAAGTG+GGG - chr7_2:87511874-87511893 None:intergenic 35.0%
! TCTCTATTTGGAATCGTGTT+AGG + chr7_2:87511914-87511933 Msa1086680:CDS 35.0%
CTAATTCCTATTACTTTGGC+TGG + chr7_2:87511938-87511957 Msa1086680:CDS 35.0%
GCATATTTACAATATCGACG+TGG + chr7_2:87511974-87511993 Msa1086680:CDS 35.0%
ATATCGACGTGGTGATCAGT+TGG + chr7_2:87511985-87512004 Msa1086680:CDS 45.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87511791 87512012 87511791 ID=Msa1086680;Name=Msa1086680
chr7_2 mRNA 87511791 87512012 87511791 ID=Msa1086680-mRNA-1;Parent=Msa1086680;Name=Msa1086680-mRNA-1;_AED=0.41;_eAED=0.66;_QI=83|0|0|1|0|0|2|0|37
chr7_2 exon 87511791 87511835 87511791 ID=Msa1086680-mRNA-1:exon:23909;Parent=Msa1086680-mRNA-1
chr7_2 exon 87511864 87512012 87511864 ID=Msa1086680-mRNA-1:exon:23910;Parent=Msa1086680-mRNA-1
chr7_2 five_prime_UTR 87511791 87511835 87511791 ID=Msa1086680-mRNA-1:five_prime_utr;Parent=Msa1086680-mRNA-1
chr7_2 five_prime_UTR 87511864 87511901 87511864 ID=Msa1086680-mRNA-1:five_prime_utr;Parent=Msa1086680-mRNA-1
chr7_2 CDS 87511902 87512012 87511902 ID=Msa1086680-mRNA-1:cds;Parent=Msa1086680-mRNA-1
Gene Sequence

>Msa1086680

ATGATTGAAGTTTCTCTATTTGGAATCGTGTTAGGTCTAATTCCTATTACTTTGGCTGGATTATTTGTAACGGCATATTTACAATATCGACGTGGTGATCAGTTGGATATT

Protein sequence

>Msa1086680

MIEVSLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDI