AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221850 MtrunA17_CPg0492821 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0221850 MtrunA17_Chr4g0016851 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0221850 MtrunA17_Chr4g0015751 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0221850 MtrunA17_Chr4g0017011 98.148 54 1 0 1 54 1 54 2.07e-33 111
Msa0221850 MtrunA17_Chr5g0423051 96.296 54 2 0 1 54 1 54 3.52e-32 107
Msa0221850 MtrunA17_Chr4g0016841 61.538 91 21 2 14 104 9 85 4.52e-31 105
Msa0221850 MtrunA17_Chr6g0451331 90.323 31 3 0 24 54 41 71 5.28e-14 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221850 ATCG00580.1 70.732 82 24 0 1 82 1 82 1.19e-34 114
Msa0221850 ATCG00560.1 97.297 37 1 0 68 104 2 38 3.75e-19 73.9

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 2_1:-70647929 Msa0221850:CDS
GTAGCTCTATAATTCGGATT+AGG 0.338134 2_1:+70647667 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 2_1:+70647963 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 2_1:-70648012 Msa0221850:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 2_1:-70648064 Msa0221850:exon
ATACCCTCCCTATTCATTGC+GGG 0.391873 2_1:-70647971 Msa0221850:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 2_1:+70647964 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.410190 2_1:-70647609 Msa0221850:CDS
CTTTCTGTAAAATACTCGTT+TGG 0.412027 2_1:+70647896 None:intergenic
TGTGTCGTAGCTCTATAATT+CGG 0.415726 2_1:+70647661 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 2_1:+70648006 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 2_1:-70648055 Msa0221850:exon
ATTCGGATTAGGTTTATCGT+TGG 0.512045 2_1:+70647678 None:intergenic
GAATCGTACCAGTCTCTACT+GGG 0.515213 2_1:-70647608 Msa0221850:CDS
TTTGACTGTATAAGAATATG+TGG 0.517465 2_1:-70648080 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 2_1:-70647950 Msa0221850:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 2_1:-70647951 Msa0221850:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 2_1:-70647967 Msa0221850:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 2_1:+70647902 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 2_1:+70647600 None:intergenic
GTATAAGAATATGTGGAGCT+CGG 0.584815 2_1:-70648073 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 2_1:-70647972 Msa0221850:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 2_1:+70647968 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 2_1:+70647967 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.711157 2_1:-70647607 Msa0221850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTAGTAGATCTTTTT+AGG - chr2_1:70647802-70647821 Msa0221850:intron 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG + chr2_1:70647992-70648011 None:intergenic 20.0%
!!! AATTCAACATTTTGTTCATT+TGG + chr2_1:70647993-70648012 None:intergenic 20.0%
! TTATTAATGGAATTCCTTGT+CGG + chr2_1:70647751-70647770 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG - chr2_1:70647805-70647824 Msa0221850:intron 25.0%
! TATTGATCCCAAAAAAAAGA+CGG + chr2_1:70647906-70647925 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG - chr2_1:70647609-70647628 Msa0221850:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + chr2_1:70647618-70647637 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG + chr2_1:70647728-70647747 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG - chr2_1:70647752-70647771 Msa0221850:intron 30.0%
GTTGTTCCAAAGAATCAAAA+CGG + chr2_1:70647778-70647797 None:intergenic 30.0%
AACCATCGTACTGTAAAAAT+GGG + chr2_1:70647857-70647876 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG - chr2_1:70647692-70647711 Msa0221850:intron 35.0%
! TATTTTACAGAAAGCCGACA+AGG - chr2_1:70647734-70647753 Msa0221850:intron 35.0%
TCAAAACGGCCAGTTATTAA+TGG + chr2_1:70647764-70647783 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG + chr2_1:70647852-70647871 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG - chr2_1:70647852-70647871 Msa0221850:intron 35.0%
CAACCATCGTACTGTAAAAA+TGG + chr2_1:70647858-70647877 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG - chr2_1:70647856-70647875 Msa0221850:intron 35.0%
! ATTCGGATTAGGTTTATCGT+TGG + chr2_1:70647946-70647965 None:intergenic 35.0%
! GTAGCTCTATAATTCGGATT+AGG + chr2_1:70647957-70647976 None:intergenic 35.0%
! TGTGTCGTAGCTCTATAATT+CGG + chr2_1:70647963-70647982 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG - chr2_1:70647649-70647668 Msa0221850:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG + chr2_1:70647660-70647679 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + chr2_1:70647661-70647680 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG + chr2_1:70647722-70647741 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG - chr2_1:70647769-70647788 Msa0221850:intron 40.0%
TAGGTCCTATCTATGGTCAT+TGG + chr2_1:70647833-70647852 None:intergenic 40.0%
AATGGGATAGGTCCTATCTA+TGG + chr2_1:70647840-70647859 None:intergenic 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG - chr2_1:70647896-70647915 Msa0221850:intron 40.0%
GATCCCAAAAAAAAGACGGT+GGG + chr2_1:70647902-70647921 None:intergenic 40.0%
TGATCCCAAAAAAAAGACGG+TGG + chr2_1:70647903-70647922 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG + chr2_1:70648024-70648043 None:intergenic 40.0%
ATACCCTCCCTATTCATTGC+GGG - chr2_1:70647650-70647669 Msa0221850:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - chr2_1:70647670-70647689 Msa0221850:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - chr2_1:70647671-70647690 Msa0221850:CDS 45.0%
AGGCTCCAATGACCATAGAT+AGG - chr2_1:70647825-70647844 Msa0221850:intron 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG - chr2_1:70647895-70647914 Msa0221850:intron 45.0%
TGAATCGTACCAGTCTCTAC+TGG - chr2_1:70648012-70648031 Msa0221850:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG - chr2_1:70648013-70648032 Msa0221850:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG - chr2_1:70648014-70648033 Msa0221850:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG + chr2_1:70647656-70647675 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG - chr2_1:70647866-70647885 Msa0221850:intron 50.0%
TATGTGGAGCTCGGCATGTC+TGG - chr2_1:70647557-70647576 Msa0221850:CDS 55.0%
CCAACCCGCAATGAATAGGG+AGG + chr2_1:70647657-70647676 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - chr2_1:70647654-70647673 Msa0221850:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG - chr2_1:70647874-70647893 Msa0221850:intron 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG - chr2_1:70647566-70647585 Msa0221850:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 70647555 70648088 70647555 ID=Msa0221850;Name=Msa0221850
chr2_1 mRNA 70647555 70648088 70647555 ID=Msa0221850-mRNA-1;Parent=Msa0221850;Name=Msa0221850-mRNA-1;_AED=0.22;_eAED=0.22;_QI=17|0|0|1|0|0|2|0|104
chr2_1 exon 70647911 70648088 70647911 ID=Msa0221850-mRNA-1:exon:20270;Parent=Msa0221850-mRNA-1
chr2_1 exon 70647555 70647705 70647555 ID=Msa0221850-mRNA-1:exon:20269;Parent=Msa0221850-mRNA-1
chr2_1 five_prime_UTR 70648072 70648088 70648072 ID=Msa0221850-mRNA-1:five_prime_utr;Parent=Msa0221850-mRNA-1
chr2_1 CDS 70647911 70648071 70647911 ID=Msa0221850-mRNA-1:cds;Parent=Msa0221850-mRNA-1
chr2_1 CDS 70647555 70647705 70647555 ID=Msa0221850-mRNA-1:cds;Parent=Msa0221850-mRNA-1
Gene Sequence

>Msa0221850

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGATTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAAT

Protein sequence

>Msa0221850

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNDSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLLLIFVLAVLFSNYFFN