AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0925030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0925030 MtrunA17_CPg0492821 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0925030 MtrunA17_Chr4g0016851 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0925030 MtrunA17_Chr4g0015751 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0925030 MtrunA17_Chr4g0017011 98.148 54 1 0 1 54 1 54 2.07e-33 111
Msa0925030 MtrunA17_Chr5g0423051 96.296 54 2 0 1 54 1 54 3.52e-32 107
Msa0925030 MtrunA17_Chr4g0016841 61.538 91 21 2 14 104 9 85 4.52e-31 105
Msa0925030 MtrunA17_Chr6g0451331 90.323 31 3 0 24 54 41 71 5.28e-14 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0925030 ATCG00580.1 70.732 82 24 0 1 82 1 82 1.19e-34 114
Msa0925030 ATCG00560.1 97.297 37 1 0 68 104 2 38 3.75e-19 73.9

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 6_2:+67575971 Msa0925030:CDS
GTAGCTCTATAATTCGGATT+AGG 0.338134 6_2:-67576233 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 6_2:-67575937 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 6_2:+67575888 Msa0925030:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 6_2:+67575836 Msa0925030:exon
ATACCCTCCCTATTCATTGC+GGG 0.391873 6_2:+67575929 Msa0925030:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 6_2:-67575936 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.410190 6_2:+67576291 Msa0925030:CDS
CTTTCTGTAAAATACTCGTT+TGG 0.412027 6_2:-67576004 None:intergenic
TGTGTCGTAGCTCTATAATT+CGG 0.415726 6_2:-67576239 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 6_2:-67575894 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 6_2:+67575845 Msa0925030:exon
ATTCGGATTAGGTTTATCGT+TGG 0.512045 6_2:-67576222 None:intergenic
GAATCGTACCAGTCTCTACT+GGG 0.515213 6_2:+67576292 Msa0925030:CDS
TTTGACTGTATAAGAATATG+TGG 0.517465 6_2:+67575820 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 6_2:+67575950 Msa0925030:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 6_2:+67575949 Msa0925030:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 6_2:+67575933 Msa0925030:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 6_2:-67575998 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 6_2:-67576300 None:intergenic
GTATAAGAATATGTGGAGCT+CGG 0.584815 6_2:+67575827 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 6_2:+67575928 Msa0925030:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 6_2:-67575932 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 6_2:-67575933 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.711157 6_2:+67576293 Msa0925030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTAGTAGATCTTTTT+AGG + chr6_2:67576081-67576100 Msa0925030:intron 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG - chr6_2:67576271-67576290 None:intergenic 20.0%
!!! AATTCAACATTTTGTTCATT+TGG - chr6_2:67576272-67576291 None:intergenic 20.0%
! TTATTAATGGAATTCCTTGT+CGG - chr6_2:67576030-67576049 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG + chr6_2:67576084-67576103 Msa0925030:intron 25.0%
! TATTGATCCCAAAAAAAAGA+CGG - chr6_2:67576185-67576204 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG + chr6_2:67575888-67575907 Msa0925030:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG - chr6_2:67575897-67575916 None:intergenic 30.0%
CTTTCTGTAAAATACTCGTT+TGG - chr6_2:67576007-67576026 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG + chr6_2:67576031-67576050 Msa0925030:intron 30.0%
GTTGTTCCAAAGAATCAAAA+CGG - chr6_2:67576057-67576076 None:intergenic 30.0%
AACCATCGTACTGTAAAAAT+GGG - chr6_2:67576136-67576155 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG + chr6_2:67575971-67575990 Msa0925030:CDS 35.0%
! TATTTTACAGAAAGCCGACA+AGG + chr6_2:67576013-67576032 Msa0925030:intron 35.0%
TCAAAACGGCCAGTTATTAA+TGG - chr6_2:67576043-67576062 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG - chr6_2:67576131-67576150 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG + chr6_2:67576131-67576150 Msa0925030:intron 35.0%
CAACCATCGTACTGTAAAAA+TGG - chr6_2:67576137-67576156 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG + chr6_2:67576135-67576154 Msa0925030:intron 35.0%
! ATTCGGATTAGGTTTATCGT+TGG - chr6_2:67576225-67576244 None:intergenic 35.0%
! GTAGCTCTATAATTCGGATT+AGG - chr6_2:67576236-67576255 None:intergenic 35.0%
! TGTGTCGTAGCTCTATAATT+CGG - chr6_2:67576242-67576261 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG + chr6_2:67575928-67575947 Msa0925030:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG - chr6_2:67575939-67575958 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG - chr6_2:67575940-67575959 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG - chr6_2:67576001-67576020 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG + chr6_2:67576048-67576067 Msa0925030:intron 40.0%
TAGGTCCTATCTATGGTCAT+TGG - chr6_2:67576112-67576131 None:intergenic 40.0%
AATGGGATAGGTCCTATCTA+TGG - chr6_2:67576119-67576138 None:intergenic 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG + chr6_2:67576175-67576194 Msa0925030:intron 40.0%
GATCCCAAAAAAAAGACGGT+GGG - chr6_2:67576181-67576200 None:intergenic 40.0%
TGATCCCAAAAAAAAGACGG+TGG - chr6_2:67576182-67576201 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG - chr6_2:67576303-67576322 None:intergenic 40.0%
ATACCCTCCCTATTCATTGC+GGG + chr6_2:67575929-67575948 Msa0925030:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG + chr6_2:67575949-67575968 Msa0925030:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG + chr6_2:67575950-67575969 Msa0925030:CDS 45.0%
AGGCTCCAATGACCATAGAT+AGG + chr6_2:67576104-67576123 Msa0925030:intron 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG + chr6_2:67576174-67576193 Msa0925030:intron 45.0%
TGAATCGTACCAGTCTCTAC+TGG + chr6_2:67576291-67576310 Msa0925030:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG + chr6_2:67576292-67576311 Msa0925030:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG + chr6_2:67576293-67576312 Msa0925030:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG - chr6_2:67575935-67575954 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG + chr6_2:67576145-67576164 Msa0925030:intron 50.0%
TATGTGGAGCTCGGCATGTC+TGG + chr6_2:67575836-67575855 Msa0925030:exon 55.0%
CCAACCCGCAATGAATAGGG+AGG - chr6_2:67575936-67575955 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG + chr6_2:67575933-67575952 Msa0925030:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG + chr6_2:67576153-67576172 Msa0925030:intron 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG + chr6_2:67575845-67575864 Msa0925030:exon 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 67575834 67576367 67575834 ID=Msa0925030;Name=Msa0925030
chr6_2 mRNA 67575834 67576367 67575834 ID=Msa0925030-mRNA-1;Parent=Msa0925030;Name=Msa0925030-mRNA-1;_AED=0.22;_eAED=0.22;_QI=17|0|0|1|0|0|2|0|104
chr6_2 exon 67575834 67576011 67575834 ID=Msa0925030-mRNA-1:exon:10706;Parent=Msa0925030-mRNA-1
chr6_2 exon 67576217 67576367 67576217 ID=Msa0925030-mRNA-1:exon:10707;Parent=Msa0925030-mRNA-1
chr6_2 five_prime_UTR 67575834 67575850 67575834 ID=Msa0925030-mRNA-1:five_prime_utr;Parent=Msa0925030-mRNA-1
chr6_2 CDS 67575851 67576011 67575851 ID=Msa0925030-mRNA-1:cds;Parent=Msa0925030-mRNA-1
chr6_2 CDS 67576217 67576367 67576217 ID=Msa0925030-mRNA-1:cds;Parent=Msa0925030-mRNA-1
Gene Sequence

>Msa0925030

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGATTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAAT

Protein sequence

>Msa0925030

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNDSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLLLIFVLAVLFSNYFFN