AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0398600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0398600 MtrunA17_CPg0492821 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0398600 MtrunA17_Chr4g0016851 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0398600 MtrunA17_Chr4g0015751 70.732 82 24 0 1 82 1 82 6.38e-34 112
Msa0398600 MtrunA17_Chr4g0017011 98.148 54 1 0 1 54 1 54 2.07e-33 111
Msa0398600 MtrunA17_Chr5g0423051 96.296 54 2 0 1 54 1 54 3.52e-32 107
Msa0398600 MtrunA17_Chr4g0016841 61.538 91 21 2 14 104 9 85 4.52e-31 105
Msa0398600 MtrunA17_Chr6g0451331 90.323 31 3 0 24 54 41 71 5.28e-14 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0398600 ATCG00580.1 70.732 82 24 0 1 82 1 82 1.19e-34 114
Msa0398600 ATCG00560.1 97.297 37 1 0 68 104 2 38 3.75e-19 73.9

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 3_2:-20504143 Msa0398600:CDS
GTAGCTCTATAATTCGGATT+AGG 0.338134 3_2:+20503881 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 3_2:+20504177 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 3_2:-20504226 Msa0398600:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 3_2:-20504278 Msa0398600:exon
ATACCCTCCCTATTCATTGC+GGG 0.391873 3_2:-20504185 Msa0398600:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 3_2:+20504178 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.410190 3_2:-20503823 Msa0398600:CDS
ATTTCTGTAAAATACTCGTT+TGG 0.410463 3_2:+20504110 None:intergenic
TGTGTCGTAGCTCTATAATT+CGG 0.415726 3_2:+20503875 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 3_2:+20504220 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 3_2:-20504269 Msa0398600:exon
ATTCGGATTAGGTTTATCGT+TGG 0.512045 3_2:+20503892 None:intergenic
GAATCGTACCAGTCTCTACT+GGG 0.515213 3_2:-20503822 Msa0398600:CDS
TTTGACTGTATAAGAATATG+TGG 0.517465 3_2:-20504294 None:intergenic
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 3_2:-20504164 Msa0398600:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 3_2:-20504165 Msa0398600:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 3_2:-20504181 Msa0398600:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 3_2:+20504116 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 3_2:+20503814 None:intergenic
GTATAAGAATATGTGGAGCT+CGG 0.584815 3_2:-20504287 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 3_2:-20504186 Msa0398600:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 3_2:+20504182 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 3_2:+20504181 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.711157 3_2:-20503821 Msa0398600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTAGTAGATCTTTTT+AGG - chr3_2:20504016-20504035 Msa0398600:intron 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG + chr3_2:20504206-20504225 None:intergenic 20.0%
!!! AATTCAACATTTTGTTCATT+TGG + chr3_2:20504207-20504226 None:intergenic 20.0%
! ATTTCTGTAAAATACTCGTT+TGG + chr3_2:20503942-20503961 None:intergenic 25.0%
! TTATTAATGGAATTCCTTGT+CGG + chr3_2:20503965-20503984 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG - chr3_2:20504019-20504038 Msa0398600:intron 25.0%
! TATTGATCCCAAAAAAAAGA+CGG + chr3_2:20504120-20504139 None:intergenic 25.0%
TTATTACCAGTATTCGATAC+TGG - chr3_2:20503823-20503842 Msa0398600:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + chr3_2:20503832-20503851 None:intergenic 30.0%
! TATTTTACAGAAATCCGACA+AGG - chr3_2:20503948-20503967 Msa0398600:intron 30.0%
CAAGGAATTCCATTAATAAC+TGG - chr3_2:20503966-20503985 Msa0398600:intron 30.0%
GTTGTTCCAAAGAATCAAAA+CGG + chr3_2:20503992-20504011 None:intergenic 30.0%
AACCATCGTACTGTAAAAAT+GGG + chr3_2:20504071-20504090 None:intergenic 30.0%
! GGTTTAGCTTACGATGTATT+TGG - chr3_2:20503906-20503925 Msa0398600:intron 35.0%
TCAAAACGGCCAGTTATTAA+TGG + chr3_2:20503978-20503997 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG + chr3_2:20504066-20504085 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG - chr3_2:20504066-20504085 Msa0398600:intron 35.0%
CAACCATCGTACTGTAAAAA+TGG + chr3_2:20504072-20504091 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG - chr3_2:20504070-20504089 Msa0398600:intron 35.0%
! ATTCGGATTAGGTTTATCGT+TGG + chr3_2:20504160-20504179 None:intergenic 35.0%
! GTAGCTCTATAATTCGGATT+AGG + chr3_2:20504171-20504190 None:intergenic 35.0%
! TGTGTCGTAGCTCTATAATT+CGG + chr3_2:20504177-20504196 None:intergenic 35.0%
TATACCCTCCCTATTCATTG+CGG - chr3_2:20503863-20503882 Msa0398600:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG + chr3_2:20503874-20503893 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + chr3_2:20503875-20503894 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG + chr3_2:20503936-20503955 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG - chr3_2:20503983-20504002 Msa0398600:intron 40.0%
TAGGTCCTATCTATGGTCAT+TGG + chr3_2:20504047-20504066 None:intergenic 40.0%
AATGGGATAGGTCCTATCTA+TGG + chr3_2:20504054-20504073 None:intergenic 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG - chr3_2:20504110-20504129 Msa0398600:intron 40.0%
GATCCCAAAAAAAAGACGGT+GGG + chr3_2:20504116-20504135 None:intergenic 40.0%
TGATCCCAAAAAAAAGACGG+TGG + chr3_2:20504117-20504136 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG + chr3_2:20504238-20504257 None:intergenic 40.0%
ATACCCTCCCTATTCATTGC+GGG - chr3_2:20503864-20503883 Msa0398600:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - chr3_2:20503884-20503903 Msa0398600:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - chr3_2:20503885-20503904 Msa0398600:CDS 45.0%
AGGCTCCAATGACCATAGAT+AGG - chr3_2:20504039-20504058 Msa0398600:intron 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG - chr3_2:20504109-20504128 Msa0398600:intron 45.0%
TGAATCGTACCAGTCTCTAC+TGG - chr3_2:20504226-20504245 Msa0398600:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG - chr3_2:20504227-20504246 Msa0398600:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG - chr3_2:20504228-20504247 Msa0398600:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG + chr3_2:20503870-20503889 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG - chr3_2:20504080-20504099 Msa0398600:intron 50.0%
TATGTGGAGCTCGGCATGTC+TGG - chr3_2:20503771-20503790 Msa0398600:CDS 55.0%
CCAACCCGCAATGAATAGGG+AGG + chr3_2:20503871-20503890 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - chr3_2:20503868-20503887 Msa0398600:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG - chr3_2:20504088-20504107 Msa0398600:intron 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG - chr3_2:20503780-20503799 Msa0398600:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 20503769 20504302 20503769 ID=Msa0398600;Name=Msa0398600
chr3_2 mRNA 20503769 20504302 20503769 ID=Msa0398600-mRNA-1;Parent=Msa0398600;Name=Msa0398600-mRNA-1;_AED=0.22;_eAED=0.22;_QI=17|0|0|1|0|0|2|0|104
chr3_2 exon 20504125 20504302 20504125 ID=Msa0398600-mRNA-1:exon:4476;Parent=Msa0398600-mRNA-1
chr3_2 exon 20503769 20503919 20503769 ID=Msa0398600-mRNA-1:exon:4475;Parent=Msa0398600-mRNA-1
chr3_2 five_prime_UTR 20504286 20504302 20504286 ID=Msa0398600-mRNA-1:five_prime_utr;Parent=Msa0398600-mRNA-1
chr3_2 CDS 20504125 20504285 20504125 ID=Msa0398600-mRNA-1:cds;Parent=Msa0398600-mRNA-1
chr3_2 CDS 20503769 20503919 20503769 ID=Msa0398600-mRNA-1:cds;Parent=Msa0398600-mRNA-1
Gene Sequence

>Msa0398600

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGATTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAAT

Protein sequence

>Msa0398600

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNDSSNDKPNPNYRATTQSNPNEQNVELNRTSLYWGLLLIFVLAVLFSNYFFN