AlfalfaGEDB | Msa1284710

M. sativa cultivar zhongmu-4 / Msa1284710

KEGG: K13237
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0016020 GO:0031090 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805
PFAM: PF00106.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284710	sp\|Q9LTV6\|DECR2_ARATH	64.983	297	55	1	6	253	2	298	1.04e-128	369

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284710	A0A072TP38	82.724	301	4	1	1	253	1	301	4.97e-164	465

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014710	Msa1284710	0.802250	6.133441e-49	-8.615850e-47
Msa0015390	Msa1284710	0.800406	1.462794e-48	-8.615850e-47
Msa0050380	Msa1284710	0.849065	4.106293e-60	-8.615850e-47
Msa0072000	Msa1284710	0.800468	1.421057e-48	-8.615850e-47
Msa0092990	Msa1284710	0.806146	9.479327e-50	-8.615850e-47
Msa0102120	Msa1284710	0.805257	1.456876e-49	-8.615850e-47
Msa0117770	Msa1284710	0.807922	3.989003e-50	-8.615850e-47
Msa0120340	Msa1284710	0.802255	6.118868e-49	-8.615850e-47
Msa0610590	Msa1284710	0.804656	1.945713e-49	-8.615850e-47
Msa0621590	Msa1284710	0.808139	3.586015e-50	-8.615850e-47
Msa0626090	Msa1284710	0.803739	3.019453e-49	-8.615850e-47
Msa0651190	Msa1284710	0.801002	1.105715e-48	-8.615850e-47
Msa0662650	Msa1284710	0.813493	2.488891e-51	-8.615850e-47
Msa0663240	Msa1284710	0.808157	3.555138e-50	-8.615850e-47
Msa0663920	Msa1284710	0.800978	1.118235e-48	-8.615850e-47
Msa0672690	Msa1284710	0.819219	1.302939e-52	-8.615850e-47
Msa0688480	Msa1284710	0.823487	1.348956e-53	-8.615850e-47
Msa0696640	Msa1284710	0.801838	7.455308e-49	-8.615850e-47
Msa0698930	Msa1284710	0.846141	2.617385e-59	-8.615850e-47
Msa0708860	Msa1284710	0.809515	1.821777e-50	-8.615850e-47
Msa0777230	Msa1284710	0.800578	1.349466e-48	-8.615850e-47
Msa0787470	Msa1284710	0.802977	4.342976e-49	-8.615850e-47
Msa0794510	Msa1284710	-0.809078	2.260550e-50	-8.615850e-47
Msa0807030	Msa1284710	0.806415	8.316985e-50	-8.615850e-47
Msa0171760	Msa1284710	0.813186	2.907266e-51	-8.615850e-47
Msa0195770	Msa1284710	0.803695	3.083743e-49	-8.615850e-47
Msa0220580	Msa1284710	0.815084	1.108045e-51	-8.615850e-47
Msa0233610	Msa1284710	0.811966	5.375095e-51	-8.615850e-47
Msa1233500	Msa1284710	0.806798	6.905665e-50	-8.615850e-47
Msa1233890	Msa1284710	0.860407	2.111179e-63	-8.615850e-47
Msa1239530	Msa1284710	0.840892	6.614279e-58	-8.615850e-47
Msa1245830	Msa1284710	0.800713	1.266480e-48	-8.615850e-47
Msa1267840	Msa1284710	0.801437	9.010168e-49	-8.615850e-47
Msa1279210	Msa1284710	0.806574	7.700493e-50	-8.615850e-47
Msa1284680	Msa1284710	0.802887	4.533295e-49	-8.615850e-47
Msa1284710	Msa1288420	0.810148	1.331105e-50	-8.615850e-47
Msa1284710	Msa1289010	0.801906	7.219102e-49	-8.615850e-47
Msa1284710	Msa1296940	0.829811	4.175975e-55	-8.615850e-47
Msa1284710	Msa1306750	0.801306	9.584248e-49	-8.615850e-47
Msa1284710	Msa1359040	0.800694	1.278029e-48	-8.615850e-47
Msa1284710	Msa1362880	0.813406	2.600928e-51	-8.615850e-47
Msa1284710	Msa1364830	0.812901	3.356850e-51	-8.615850e-47
Msa1284710	Msa1376760	0.814825	1.265060e-51	-8.615850e-47
Msa1284710	Msa1386430	0.814000	1.925350e-51	-8.615850e-47
Msa1284710	Msa1408790	0.805642	1.209697e-49	-8.615850e-47
Msa1284710	Msa1411020	0.816452	5.490842e-52	-8.615850e-47
Msa1284710	Msa1420490	0.811280	7.574545e-51	-8.615850e-47
Msa1284710	Msa1423390	0.810714	1.004909e-50	-8.615850e-47
Msa1284710	Msa1424290	0.809234	2.092915e-50	-8.615850e-47
Msa1284710	Msa1427230	0.801172	1.020708e-48	-8.615850e-47
Msa1284710	Msa1434350	0.812338	4.457302e-51	-8.615850e-47
Msa1284710	Msa1436040	0.805109	1.564328e-49	-8.615850e-47
Msa1284710	Msa1439320	0.813831	2.097553e-51	-8.615850e-47
Msa1284710	Msa1440370	0.805322	1.411762e-49	-8.615850e-47
Msa1284710	Msa1440810	0.810322	1.221048e-50	-8.615850e-47
Msa1284710	Msa1444740	0.801236	9.902305e-49	-8.615850e-47
Msa1284710	Msa1451620	-0.833902	4.084806e-56	-8.615850e-47
Msa1284710	Msa1453560	0.810234	1.275701e-50	-8.615850e-47
Msa1284710	Msa1458550	0.819768	9.764763e-53	-8.615850e-47
Msa0365490	Msa1284710	0.818312	2.093001e-52	-8.615850e-47
Msa0376590	Msa1284710	0.824287	8.761468e-54	-8.615850e-47
Msa0379640	Msa1284710	0.819536	1.103024e-52	-8.615850e-47
Msa0381620	Msa1284710	-0.807312	5.375561e-50	-8.615850e-47
Msa0405370	Msa1284710	0.814631	1.396259e-51	-8.615850e-47
Msa0426710	Msa1284710	0.801763	7.723971e-49	-8.615850e-47
Msa0429290	Msa1284710	0.806682	7.305544e-50	-8.615850e-47
Msa0431670	Msa1284710	0.800080	1.704356e-48	-8.615850e-47
Msa0442850	Msa1284710	0.817121	3.886571e-52	-8.615850e-47
Msa0449590	Msa1284710	-0.804017	2.643009e-49	-8.615850e-47
Msa0458440	Msa1284710	0.824177	9.298529e-54	-8.615850e-47
Msa0466390	Msa1284710	0.800578	1.349831e-48	-8.615850e-47
Msa0473240	Msa1284710	0.827729	1.332136e-54	-8.615850e-47
Msa0991930	Msa1284710	0.807491	4.925814e-50	-8.615850e-47
Msa0992280	Msa1284710	0.822602	2.169767e-53	-8.615850e-47
Msa1015730	Msa1284710	0.817084	3.961001e-52	-8.615850e-47
Msa1015790	Msa1284710	0.826633	2.437588e-54	-8.615850e-47
Msa1015810	Msa1284710	0.814339	1.620457e-51	-8.615850e-47
Msa1026760	Msa1284710	0.802180	6.341193e-49	-8.615850e-47
Msa1026790	Msa1284710	0.812471	4.170459e-51	-8.615850e-47
Msa1028970	Msa1284710	0.822623	2.145344e-53	-8.615850e-47
Msa1034300	Msa1284710	0.810194	1.301239e-50	-8.615850e-47
Msa1039880	Msa1284710	0.802280	6.045533e-49	-8.615850e-47
Msa1059570	Msa1284710	0.832136	1.122974e-55	-8.615850e-47
Msa1061020	Msa1284710	-0.801424	9.064927e-49	-8.615850e-47
Msa1071100	Msa1284710	0.814939	1.193329e-51	-8.615850e-47
Msa1071110	Msa1284710	0.821642	3.622600e-53	-8.615850e-47
Msa1075080	Msa1284710	-0.822065	2.890938e-53	-8.615850e-47
Msa1075220	Msa1284710	-0.818679	1.728809e-52	-8.615850e-47
Msa1081590	Msa1284710	0.800417	1.455296e-48	-8.615850e-47
Msa1082500	Msa1284710	0.801352	9.375722e-49	-8.615850e-47
Msa1082580	Msa1284710	0.837475	5.085301e-57	-8.615850e-47
Msa1084260	Msa1284710	0.800219	1.597005e-48	-8.615850e-47
Msa1087470	Msa1284710	0.808959	2.396406e-50	-8.615850e-47
Msa0266690	Msa1284710	0.823061	1.696693e-53	-8.615850e-47
Msa0266720	Msa1284710	0.836559	8.719349e-57	-8.615850e-47
Msa0269450	Msa1284710	0.808525	2.967803e-50	-8.615850e-47
Msa0288100	Msa1284710	0.837104	6.328714e-57	-8.615850e-47
Msa0344870	Msa1284710	0.804492	2.104909e-49	-8.615850e-47
Msa0350990	Msa1284710	0.834784	2.453024e-56	-8.615850e-47
Msa0351030	Msa1284710	0.833347	5.617958e-56	-8.615850e-47
Msa0351100	Msa1284710	0.822549	2.231966e-53	-8.615850e-47
Msa0351810	Msa1284710	0.808021	3.799539e-50	-8.615850e-47
Msa1117170	Msa1284710	0.803380	3.584000e-49	-8.615850e-47
Msa1122250	Msa1284710	0.805010	1.640600e-49	-8.615850e-47
Msa1139000	Msa1284710	-0.803880	2.822491e-49	-8.615850e-47
Msa1148050	Msa1284710	0.821955	3.066559e-53	-8.615850e-47
Msa1161730	Msa1284710	0.810954	8.915816e-51	-8.615850e-47
Msa1162550	Msa1284710	-0.824448	8.027606e-54	-8.615850e-47
Msa1174040	Msa1284710	0.816813	4.556504e-52	-8.615850e-47
Msa1181550	Msa1284710	0.812564	3.979083e-51	-8.615850e-47
Msa1199560	Msa1284710	0.803748	3.006754e-49	-8.615850e-47
Msa0516370	Msa1284710	0.825101	5.631363e-54	-8.615850e-47
Msa0517110	Msa1284710	0.811485	6.839902e-51	-8.615850e-47
Msa0578970	Msa1284710	0.805968	1.033100e-49	-8.615850e-47
Msa0585230	Msa1284710	0.822807	1.944035e-53	-8.615850e-47
Msa0598460	Msa1284710	0.811570	6.554203e-51	-8.615850e-47
Msa0893810	Msa1284710	0.801868	7.348463e-49	-8.615850e-47
Msa0919850	Msa1284710	0.817542	3.123733e-52	-8.615850e-47
Msa0930400	Msa1284710	-0.809187	2.141606e-50	-8.615850e-47
Msa0931520	Msa1284710	0.818393	2.007113e-52	-8.615850e-47
Msa0935540	Msa1284710	0.801458	8.918750e-49	-8.615850e-47
Msa0967180	Msa1284710	0.830512	2.816432e-55	-8.615850e-47
Msa0969520	Msa1284710	0.806952	6.407997e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284710	MtrunA17_Chr8g0357481	82.724	301	4	1	1	253	1	301	9.57e-168	465
Msa1284710	MtrunA17_Chr4g0044131	32.530	249	119	8	14	216	8	253	2.28e-17	79.3
Msa1284710	MtrunA17_Chr7g0244061	28.723	282	134	8	4	221	19	297	3.44e-17	79.3
Msa1284710	MtrunA17_Chr4g0026001	27.863	262	129	9	14	225	14	265	2.43e-16	76.6
Msa1284710	MtrunA17_Chr4g0075531	26.613	248	138	3	11	217	4	248	4.25e-16	75.5
Msa1284710	MtrunA17_Chr7g0239461	27.778	216	127	8	14	217	16	214	1.02e-15	73.9
Msa1284710	MtrunA17_Chr7g0267001	33.333	231	123	8	14	216	8	235	1.87e-14	70.9
Msa1284710	MtrunA17_Chr8g0386171	44.944	89	48	1	14	101	52	140	3.32e-14	71.2
Msa1284710	MtrunA17_Chr8g0386201	32.353	136	90	2	9	142	40	175	8.76e-14	70.1
Msa1284710	MtrunA17_Chr1g0182511	25.856	263	147	7	1	216	1	262	1.19e-13	68.9
Msa1284710	MtrunA17_Chr3g0112171	25.610	246	140	8	14	217	15	259	7.93e-11	60.8
Msa1284710	MtrunA17_Chr7g0267011	35.593	118	72	2	14	129	8	123	8.53e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284710	AT3G12800.1	64.983	297	55	1	6	253	2	298	1.06e-129	369
Msa1284710	AT2G07640.1	50.667	150	25	1	131	231	1	150	1.99e-40	137
Msa1284710	AT1G36580.1	69.841	63	19	0	180	242	1	63	9.45e-25	97.8
Msa1284710	AT1G52340.1	28.788	264	135	8	14	224	18	281	3.68e-20	87.4
Msa1284710	AT1G54870.1	28.462	260	137	7	5	216	72	330	6.53e-20	87.8
Msa1284710	AT1G54870.2	28.462	260	137	7	5	216	74	332	6.74e-20	87.8
Msa1284710	AT4G05530.1	30.769	247	129	6	11	217	6	250	1.31e-19	85.5
Msa1284710	AT3G05260.1	27.757	263	142	7	5	220	27	288	2.54e-19	85.5
Msa1284710	AT1G63380.1	30.435	253	125	9	14	216	17	268	2.10e-17	80.1
Msa1284710	AT1G63380.4	30.435	253	125	9	14	216	19	270	2.41e-17	79.7
Msa1284710	AT1G63380.3	30.435	253	125	9	14	216	15	266	2.48e-17	79.7
Msa1284710	AT1G63380.2	30.435	253	125	9	14	216	22	273	2.96e-17	79.7
Msa1284710	AT5G50700.1	36.154	130	81	2	14	141	45	174	6.73e-17	79.3
Msa1284710	AT5G50600.1	36.154	130	81	2	14	141	45	174	6.73e-17	79.3
Msa1284710	AT3G46170.1	32.941	255	115	12	14	216	26	276	9.25e-15	72.8
Msa1284710	AT2G47130.1	28.405	257	130	9	14	222	6	256	3.06e-13	68.2
Msa1284710	AT3G47350.1	32.061	131	87	2	14	142	44	174	4.40e-13	68.2
Msa1284710	AT3G47350.2	32.061	131	87	2	14	142	44	174	8.61e-13	67.4
Msa1284710	AT3G47360.1	32.031	128	85	2	17	142	48	175	1.41e-12	66.6
Msa1284710	AT4G10020.1	30.657	137	93	2	7	141	40	176	1.81e-12	66.6
Msa1284710	AT3G47350.3	35.644	101	64	1	14	113	44	144	3.39e-11	62.4

Find 93 sgRNAs with CRISPR-Local

Find 319 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACCAATTGGTTCATTTC+TGG	0.141423	8_3:+27372124	None:intergenic
TGTGAAGGAAAATACATTAA+TGG	0.230658	8_3:-27372245	Msa1284710:intron
ATGCCAGGAGTGTCACTTAT+TGG	0.283238	8_3:+27374295	None:intergenic
CGCTTTCGATTCCAGCTGTT+GGG	0.292110	8_3:-27376329	Msa1284710:CDS
TTCAAACCAGATATCTTAAA+AGG	0.308568	8_3:-27376489	Msa1284710:CDS
ACTGGCGGAGCGTCGGGAAT+TGG	0.320337	8_3:-27376450	Msa1284710:CDS
GGCAAAGTAGCTTTAATAAC+TGG	0.335212	8_3:-27376468	Msa1284710:CDS
TGTATAAACTTGGGGAGAAA+TGG	0.343265	8_3:-27374219	Msa1284710:CDS
CCTCTGCTACCTATTTCTTC+AGG	0.344151	8_3:+27374259	None:intergenic
CATCCAACAGAAGAATAAAA+AGG	0.351586	8_3:-27371919	Msa1284710:three_prime_UTR
GTTTGAAATATCAACGCAAT+TGG	0.363203	8_3:-27376427	Msa1284710:CDS
AGTTCTTCAATCTGCGGTTT+CGG	0.369174	8_3:-27376361	Msa1284710:CDS
AGTCACTTTCATTTCATGAT+TGG	0.373071	8_3:-27371773	Msa1284710:three_prime_UTR
TTTGAAATATCAACGCAATT+GGG	0.380854	8_3:-27376426	Msa1284710:CDS
TGCGGCGAGAGTTGTTGATT+TGG	0.388704	8_3:-27376277	Msa1284710:CDS
CTGAGGATCTTTCGCCGAAT+GGG	0.391331	8_3:-27376188	Msa1284710:CDS
GATTATGATATTCGAGTCAA+TGG	0.397358	8_3:-27374331	Msa1284710:CDS
ATTCCAGCTGTTGGGTTTGA+AGG	0.400062	8_3:-27376321	Msa1284710:CDS
AAGCTATGAGCCTGCAGATT+GGG	0.410407	8_3:-27372092	Msa1284710:exon
CACTCCTGGCATGAGTAAAC+TGG	0.411577	8_3:-27374284	Msa1284710:CDS
ATCTGGTTTGAAGGGTGATT+CGG	0.416889	8_3:+27376500	None:intergenic
AAACTGGCTCCTGAAGAAAT+AGG	0.417404	8_3:-27374268	Msa1284710:CDS
TTTGTACTATCTTTGCCTGT+TGG	0.425593	8_3:-27371870	Msa1284710:three_prime_UTR
TCGCTTTCGATTCCAGCTGT+TGG	0.427172	8_3:-27376330	Msa1284710:CDS
GGCGACACTATGATAGTTGA+TGG	0.432277	8_3:-27372224	Msa1284710:CDS
GTATAAACTTGGGGAGAAAT+GGG	0.441031	8_3:-27374218	Msa1284710:CDS
ATGGAGGACTTTGGCTGAGC+CGG	0.441556	8_3:-27372205	Msa1284710:CDS
CATAAATTATGTTTCTAAGC+TGG	0.441961	8_3:+27371962	None:intergenic
TTTAATAACTGGCGGAGCGT+CGG	0.442410	8_3:-27376457	Msa1284710:CDS
TGAAGGGGATGTGAGGAAGC+AGG	0.455697	8_3:-27376304	Msa1284710:CDS
GCTGAGGATCTTTCGCCGAA+TGG	0.457228	8_3:-27376189	Msa1284710:CDS
ATTCTTGTTAATGCTGCTGC+TGG	0.464854	8_3:-27376228	Msa1284710:CDS
ACCTGTCTCACTGCCTCCTT+CGG	0.468095	8_3:+27372170	None:intergenic
AATTTGCTTACAATTCCAAC+AGG	0.468165	8_3:+27371855	None:intergenic
GAAATGCCTCTGTATAAACT+TGG	0.470825	8_3:-27374229	Msa1284710:CDS
TGGAGCGTCGGTTGCTGTGA+TGG	0.472642	8_3:-27376397	Msa1284710:CDS
TTCCAGCTGTTGGGTTTGAA+GGG	0.474545	8_3:-27376320	Msa1284710:CDS
CACTTAAGTATCTCAAGAAA+GGG	0.475461	8_3:-27374720	Msa1284710:CDS
AGATCCAGAAATGAACCAAT+TGG	0.476611	8_3:-27372128	Msa1284710:CDS
GCACTTAAGTATCTCAAGAA+AGG	0.480331	8_3:-27374721	Msa1284710:CDS
GCCGAAGGAGGCAGTGAGAC+AGG	0.494400	8_3:-27372171	Msa1284710:CDS
TATTAGCCCCTCTACACTGC+AGG	0.498100	8_3:-27372069	Msa1284710:three_prime_UTR
AAATGCCTCTGTATAAACTT+GGG	0.505018	8_3:-27374228	Msa1284710:CDS
TCCCCTTCAAACCCAACAGC+TGG	0.506137	8_3:+27376318	None:intergenic
CTCTACTTAGCATCAGATGC+AGG	0.507649	8_3:-27374178	Msa1284710:intron
TCATGAAATGAAAGTGACTA+AGG	0.508457	8_3:+27371777	None:intergenic
TTTAAGATATCTGGTTTGAA+GGG	0.515723	8_3:+27376492	None:intergenic
ATTATGATATTCGAGTCAAT+GGG	0.519870	8_3:-27374330	Msa1284710:CDS
TGATAGTTGATGGAGGACTT+TGG	0.521104	8_3:-27372214	Msa1284710:CDS
GGAGCCAGTTTACTCATGCC+AGG	0.525918	8_3:+27374280	None:intergenic
CGAGTCAATGGGATTGCACC+AGG	0.526292	8_3:-27374319	Msa1284710:CDS
TCACCTGTTCGAAACCCATT+CGG	0.526674	8_3:+27376174	None:intergenic
TAAGCTATGAGCCTGCAGAT+TGG	0.533407	8_3:-27372093	Msa1284710:exon
TAGTACAAACACACAACCAT+GGG	0.533420	8_3:+27371884	None:intergenic
AGTAATCCCTGCAGTGTAGA+GGG	0.534640	8_3:+27372062	None:intergenic
GGGGCTAATACCCAATCTGC+AGG	0.535806	8_3:+27372082	None:intergenic
GCGAATTTAGCGTCGAAACA+TGG	0.537122	8_3:-27376532	Msa1284710:exon
TTGGGTTTGAAGGGGATGTG+AGG	0.552630	8_3:-27376311	Msa1284710:CDS
CGGTTGCTGTGATGGGTCGT+CGG	0.552646	8_3:-27376389	Msa1284710:CDS
GGAGCGTCGGTTGCTGTGAT+GGG	0.554476	8_3:-27376396	Msa1284710:CDS
TCCAGCTGTTGGGTTTGAAG+GGG	0.555982	8_3:-27376319	Msa1284710:CDS
TATCTCAAGAAAGGGGCACC+TGG	0.556489	8_3:-27374712	Msa1284710:intron
TTCTTGTTAATGCTGCTGCT+GGG	0.557653	8_3:-27376227	Msa1284710:CDS
GCCACGTTATTTGCCGAAGG+AGG	0.559286	8_3:-27372183	Msa1284710:CDS
TCAACGCAATTGGGGAAACA+TGG	0.567028	8_3:-27376417	Msa1284710:CDS
TTGAAATATCAACGCAATTG+GGG	0.574459	8_3:-27376425	Msa1284710:CDS
GGAGTGTCACTTATTGGACC+TGG	0.583283	8_3:+27374301	None:intergenic
CCTGAAGAAATAGGTAGCAG+AGG	0.588385	8_3:-27374259	Msa1284710:CDS
AAGTAATCCCTGCAGTGTAG+AGG	0.601402	8_3:+27372061	None:intergenic
CCTTCGGCAAATAACGTGGC+CGG	0.605481	8_3:+27372186	None:intergenic
AAGGCTATGGCTAGACCCCA+TGG	0.605801	8_3:-27371900	Msa1284710:three_prime_UTR
AATGCCTCTGTATAAACTTG+GGG	0.607132	8_3:-27374227	Msa1284710:CDS
GAAGCAAGTTCTTCAATCTG+CGG	0.609928	8_3:-27376367	Msa1284710:CDS
TGTGAGGAAGCAGGAAGATG+CGG	0.618720	8_3:-27376295	Msa1284710:CDS
TTCTCCCCAAGTTTATACAG+AGG	0.622115	8_3:+27374223	None:intergenic
GTTTCAGACACCAATGTGTG+TGG	0.633582	8_3:+27376642	None:intergenic
GGTCCAATAAGTGACACTCC+TGG	0.636505	8_3:-27374298	Msa1284710:CDS
GCCTCCTTCGGCAAATAACG+TGG	0.636778	8_3:+27372182	None:intergenic
ATAGTACAAACACACAACCA+TGG	0.639923	8_3:+27371883	None:intergenic
GAATTGAATCAACGCAGTAG+CGG	0.640289	8_3:-27376554	Msa1284710:five_prime_UTR
ATTGGGGAAACATGGAGCGT+CGG	0.642414	8_3:-27376409	Msa1284710:CDS
GGAGAAATGGGATATTGCAA+TGG	0.644314	8_3:-27374206	Msa1284710:CDS
ATTAGCCCCTCTACACTGCA+GGG	0.653439	8_3:-27372068	Msa1284710:three_prime_UTR
GTGTTGGATATCGATTCTGT+TGG	0.666144	8_3:-27374766	Msa1284710:CDS
AAAGTAGCTTTAATAACTGG+CGG	0.668949	8_3:-27376465	Msa1284710:CDS
GTAACTTTGAACATGAAATG+TGG	0.673300	8_3:-27371827	Msa1284710:three_prime_UTR
GTAAAAGACTCCACACACAT+TGG	0.673912	8_3:-27376652	Msa1284710:five_prime_UTR
TACTTCTTTAAACAACACAG+TGG	0.675711	8_3:-27372043	Msa1284710:three_prime_UTR
ACTTAAGTATCTCAAGAAAG+GGG	0.678718	8_3:-27374719	Msa1284710:CDS
TGAACATGAAATGTGGCTAG+TGG	0.680977	8_3:-27371820	Msa1284710:three_prime_UTR
GTAATCCCTGCAGTGTAGAG+GGG	0.684512	8_3:+27372063	None:intergenic
GACACTATGATAGTTGATGG+AGG	0.701596	8_3:-27372221	Msa1284710:CDS
AGTACAAACACACAACCATG+GGG	0.762696	8_3:+27371885	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTAATTTAAGGATTTAA+AGG	-	chr8_3:27373829-27373848	Msa1284710:intron	10.0%
!!!	TAAAATTTTAATTATTCAAC+AGG	-	chr8_3:27373988-27374007	Msa1284710:intron	10.0%
!!	AGATATAATTATGTAATTAT+AGG	-	chr8_3:27374556-27374575	Msa1284710:intron	10.0%
!!	GATATAATTATGTAATTATA+GGG	-	chr8_3:27374557-27374576	Msa1284710:intron	10.0%
!!	ATAAAAAAATAATGCAAAAA+AGG	+	chr8_3:27374592-27374611	None:intergenic	10.0%
!!!	TTTTTTGCATTATTTTTTTA+TGG	-	chr8_3:27374591-27374610	Msa1284710:intron	10.0%
!!!	AAAAAACAAGTTAAAATTTT+GGG	+	chr8_3:27375750-27375769	None:intergenic	10.0%
!!!	AAAAAAACAAGTTAAAATTT+TGG	+	chr8_3:27375751-27375770	None:intergenic	10.0%
!!	CAAATCAATAAAAAAATTAA+AGG	+	chr8_3:27376095-27376114	None:intergenic	10.0%
!!!	TTACTTTTTAGATTCAATTA+AGG	-	chr8_3:27372298-27372317	Msa1284710:intron	15.0%
!!!	TTCTTTCATTTGTAATTTTA+AGG	+	chr8_3:27372495-27372514	None:intergenic	15.0%
!!!	ATATGTAGATTTTAAAATCA+AGG	+	chr8_3:27373114-27373133	None:intergenic	15.0%
!!	AAAGATAAAATTTAACATCT+AGG	+	chr8_3:27373812-27373831	None:intergenic	15.0%
!!!	GCAAAATAATATATTTTCAT+TGG	+	chr8_3:27373893-27373912	None:intergenic	15.0%
!!	AAGCTTAAATTAAGTTTAAT+GGG	+	chr8_3:27373969-27373988	None:intergenic	15.0%
!!	TAAGCTTAAATTAAGTTTAA+TGG	+	chr8_3:27373970-27373989	None:intergenic	15.0%
!!	CAATTTATATTTGTAGTATA+AGG	+	chr8_3:27374627-27374646	None:intergenic	15.0%
!!	TATACTACAAATATAAATTG+TGG	-	chr8_3:27374627-27374646	Msa1284710:intron	15.0%
!!	ATTACAAAAATACGAAAAAA+TGG	+	chr8_3:27374677-27374696	None:intergenic	15.0%
!!!	TTTATTTTTCTACTTTTAGT+AGG	-	chr8_3:27375022-27375041	Msa1284710:intron	15.0%
!!!	TAATAAAATGAAATTTTGCA+AGG	-	chr8_3:27375871-27375890	Msa1284710:intron	15.0%
!!!	TTTTTTTATTGATTTGTCTA+AGG	-	chr8_3:27376099-27376118	Msa1284710:intron	15.0%
!!	GAATCTAAAAAGTAAAATTG+AGG	+	chr8_3:27372293-27372312	None:intergenic	20.0%
!!	ATTCAATTAAGGTATGTATT+TGG	-	chr8_3:27372309-27372328	Msa1284710:intron	20.0%
!!!	TGTCTATTTAGTAGTTATTT+TGG	-	chr8_3:27372436-27372455	Msa1284710:intron	20.0%
!!	AAATTGCAAATACATTTCTT+AGG	+	chr8_3:27372627-27372646	None:intergenic	20.0%
!!	ATTTGAATTGCATAAAATCA+TGG	-	chr8_3:27373141-27373160	Msa1284710:intron	20.0%
!!!	AAATTCTACAACTTTTTGAA+TGG	+	chr8_3:27373270-27373289	None:intergenic	20.0%
!!!	AATGATTTTGCAATAATCTA+AGG	+	chr8_3:27373329-27373348	None:intergenic	20.0%
!!!	AAGTTATGAAAATGTTTCTA+AGG	+	chr8_3:27374330-27374349	None:intergenic	20.0%
!!!	AAGTTATGAAAATGTTTCTA+AGG	+	chr8_3:27374330-27374349	None:intergenic	20.0%
!!	GTTGTAAAACTAGAAATTAT+AGG	-	chr8_3:27374527-27374546	Msa1284710:intron	20.0%
!!	TATATTTGTAGTATAAGGAT+AGG	+	chr8_3:27374622-27374641	None:intergenic	20.0%
!!	TTAAATAAAACATACGATGA+GGG	+	chr8_3:27374907-27374926	None:intergenic	20.0%
!!	TTTAAATAAAACATACGATG+AGG	+	chr8_3:27374908-27374927	None:intergenic	20.0%
!!	CTAAAAAAGACATGATAAAT+TGG	+	chr8_3:27375098-27375117	None:intergenic	20.0%
!!!	TTGTAACAAATACTCTATTT+TGG	-	chr8_3:27375345-27375364	Msa1284710:intron	20.0%
!!	ACAAAACCTTCTTAAAATTT+GGG	-	chr8_3:27375519-27375538	Msa1284710:intron	20.0%
!!	ATGTAGTGTTTATAATGTTT+GGG	+	chr8_3:27375592-27375611	None:intergenic	20.0%
!!	TATGTAGTGTTTATAATGTT+TGG	+	chr8_3:27375593-27375612	None:intergenic	20.0%
!!	AAACATTATAAACACTACAT+AGG	-	chr8_3:27375592-27375611	Msa1284710:intron	20.0%
!!!	AAAATCTAGAATGCATAATA+AGG	+	chr8_3:27375824-27375843	None:intergenic	20.0%
!!!	ACAATCTGATAATTTTAAGA+TGG	+	chr8_3:27375939-27375958	None:intergenic	20.0%
!!	TTTAAGATATCTGGTTTGAA+GGG	+	chr8_3:27371900-27371919	None:intergenic	25.0%
!!!	TTTTAAGATATCTGGTTTGA+AGG	+	chr8_3:27371901-27371920	None:intergenic	25.0%
!	TTCAAACCAGATATCTTAAA+AGG	-	chr8_3:27371900-27371919	Msa1284710:three_prime_UTR	25.0%
!!	TTTGAAATATCAACGCAATT+GGG	-	chr8_3:27371963-27371982	Msa1284710:three_prime_UTR	25.0%
!!!	ATTTGGCTTTTAAGCATTTT+GGG	-	chr8_3:27372129-27372148	Msa1284710:CDS	25.0%
!	CTAAAAAGTAAAATTGAGGA+CGG	+	chr8_3:27372289-27372308	None:intergenic	25.0%
!	CATCAAATAGCTAATGTATA+TGG	+	chr8_3:27372348-27372367	None:intergenic	25.0%
!	TGATTTGAATAAGTGTCTAT+AGG	-	chr8_3:27372558-27372577	Msa1284710:intron	25.0%
!	AGGCTAAACAACTTAAAAAA+AGG	+	chr8_3:27372698-27372717	None:intergenic	25.0%
!!!	AGTTGTTTAGCCTTTTTAAA+CGG	-	chr8_3:27372705-27372724	Msa1284710:intron	25.0%
!	ACTTACTTAACCGTTTAAAA+AGG	+	chr8_3:27372718-27372737	None:intergenic	25.0%
!!!	AGCTTTGGTTAATAGATTTT+GGG	-	chr8_3:27372762-27372781	Msa1284710:intron	25.0%
!!!	CTACTATCATAAGTTTTGTA+AGG	-	chr8_3:27372814-27372833	Msa1284710:intron	25.0%
!!!	ATAAGTTTTGTAAGGTTGTT+TGG	-	chr8_3:27372822-27372841	Msa1284710:intron	25.0%
!!!	TAAGTTTTGTAAGGTTGTTT+GGG	-	chr8_3:27372823-27372842	Msa1284710:intron	25.0%
!!	TTATAAGTTTTTATCCAAGC+AGG	-	chr8_3:27373018-27373037	Msa1284710:intron	25.0%
!!	TATAAGTTTTTATCCAAGCA+GGG	-	chr8_3:27373019-27373038	Msa1284710:intron	25.0%
!	GTTAATCATGTTTAAATCTG+TGG	+	chr8_3:27373092-27373111	None:intergenic	25.0%
!	GGTTAATTAAGTTCAATTGA+TGG	-	chr8_3:27373162-27373181	Msa1284710:intron	25.0%
!!	AACTTTTTGAATGGTTAACA+CGG	+	chr8_3:27373261-27373280	None:intergenic	25.0%
!!	ATGCTATTTGAACATCAATT+TGG	+	chr8_3:27373299-27373318	None:intergenic	25.0%
!	TGTAAATTAGATAAGAGTCT+TGG	+	chr8_3:27373522-27373541	None:intergenic	25.0%
!!	CATTTCTCGTATTCAATTTT+AGG	+	chr8_3:27373572-27373591	None:intergenic	25.0%
!	ATTATGATATTCGAGTCAAT+GGG	-	chr8_3:27374059-27374078	Msa1284710:intron	25.0%
!!!	CTGTAAATGTTGTATTTTTG+TGG	-	chr8_3:27374285-27374304	Msa1284710:CDS	25.0%
!	CATGATTAATAAGTAGTGTT+TGG	-	chr8_3:27374447-27374466	Msa1284710:intron	25.0%
!	ATTAATAAGTAGTGTTTGGT+TGG	-	chr8_3:27374451-27374470	Msa1284710:intron	25.0%
!	TTAATAAGTAGTGTTTGGTT+GGG	-	chr8_3:27374452-27374471	Msa1284710:intron	25.0%
!	TTATGTAATTATAGGGAAGT+AGG	-	chr8_3:27374564-27374583	Msa1284710:intron	25.0%
!	TGTAATACGTATTTAAACCT+TGG	-	chr8_3:27374691-27374710	Msa1284710:intron	25.0%
!	TATTAAAGAGTGGAATACAA+AGG	+	chr8_3:27375258-27375277	None:intergenic	25.0%
!!!	TTATTTCTAGGTGATCTAAA+TGG	+	chr8_3:27375437-27375456	None:intergenic	25.0%
!	AAACCTTCTTAATCACTTTA+CGG	-	chr8_3:27375486-27375505	Msa1284710:intron	25.0%
!	GACAAAACCTTCTTAAAATT+TGG	-	chr8_3:27375518-27375537	Msa1284710:intron	25.0%
!!	TAAAATGAAATTTTGCAAGG+TGG	-	chr8_3:27375874-27375893	Msa1284710:intron	25.0%
!!	AAAATGAAATTTTGCAAGGT+GGG	-	chr8_3:27375875-27375894	Msa1284710:intron	25.0%
!	AGTATTCAGTGAGTAAAAAT+GGG	+	chr8_3:27376004-27376023	None:intergenic	25.0%
!	TAGTATTCAGTGAGTAAAAA+TGG	+	chr8_3:27376005-27376024	None:intergenic	25.0%
!	ACAATCGTTTAAATTTGTGA+AGG	-	chr8_3:27376129-27376148	Msa1284710:intron	25.0%
!	TGTGAAGGAAAATACATTAA+TGG	-	chr8_3:27376144-27376163	Msa1284710:intron	25.0%
!!	CATAAATTATGTTTCTAAGC+TGG	+	chr8_3:27376430-27376449	None:intergenic	25.0%
!!!	ACTTTGCCTTTTAAGATATC+TGG	+	chr8_3:27371909-27371928	None:intergenic	30.0%
!!	AAAGTAGCTTTAATAACTGG+CGG	-	chr8_3:27371924-27371943	Msa1284710:three_prime_UTR	30.0%
!	GTTTGAAATATCAACGCAAT+TGG	-	chr8_3:27371962-27371981	Msa1284710:three_prime_UTR	30.0%
	TTGAAATATCAACGCAATTG+GGG	-	chr8_3:27371964-27371983	Msa1284710:three_prime_UTR	30.0%
!!!	GATTTGGCTTTTAAGCATTT+TGG	-	chr8_3:27372128-27372147	Msa1284710:CDS	30.0%
!!!	AGGATTTTTGTTGTTTTCGA+TGG	-	chr8_3:27372237-27372256	Msa1284710:CDS	30.0%
	CTAGATAGAGTATGAATATC+TGG	-	chr8_3:27372404-27372423	Msa1284710:intron	30.0%
	ATTTCTTAGGAATCGAGATT+GGG	+	chr8_3:27372614-27372633	None:intergenic	30.0%
	TTTGCAATTTCGATGCATTT+AGG	-	chr8_3:27372637-27372656	Msa1284710:intron	30.0%
	TTGCAATTTCGATGCATTTA+GGG	-	chr8_3:27372638-27372657	Msa1284710:intron	30.0%
!!	CAGCTTTGGTTAATAGATTT+TGG	-	chr8_3:27372761-27372780	Msa1284710:intron	30.0%
!!	GTTGTTTTCGTAAGCTATAT+AGG	+	chr8_3:27372913-27372932	None:intergenic	30.0%
	AGCTTACGAAAACAACTTAT+AGG	-	chr8_3:27372917-27372936	Msa1284710:intron	30.0%
	TTCATTATTGTTTGCAGTGT+TGG	-	chr8_3:27373607-27373626	Msa1284710:intron	30.0%
	CACTTAAGTATCTCAAGAAA+GGG	-	chr8_3:27373669-27373688	Msa1284710:intron	30.0%
	ACTTAAGTATCTCAAGAAAG+GGG	-	chr8_3:27373670-27373689	Msa1284710:intron	30.0%
!	AGTATAGATCAGTTTTACAC+TGG	-	chr8_3:27373864-27373883	Msa1284710:intron	30.0%
	GATTATGATATTCGAGTCAA+TGG	-	chr8_3:27374058-27374077	Msa1284710:intron	30.0%
	AAATGCCTCTGTATAAACTT+GGG	-	chr8_3:27374161-27374180	Msa1284710:intron	30.0%
	TATACAAAGTAGAGAACACT+TGG	+	chr8_3:27374258-27374277	None:intergenic	30.0%
!!	AATGTTGTATTTTTGTGGTG+AGG	-	chr8_3:27374290-27374309	Msa1284710:CDS	30.0%
	TAATAAGTAGTGTTTGGTTG+GGG	-	chr8_3:27374453-27374472	Msa1284710:intron	30.0%
	TTTGTAGTATAAGGATAGGA+AGG	+	chr8_3:27374618-27374637	None:intergenic	30.0%
!!	TAACAGAGAAAAGTATTGAC+AGG	+	chr8_3:27374733-27374752	None:intergenic	30.0%
!!!	TCATGACTTTTTTGACATGT+TGG	-	chr8_3:27374758-27374777	Msa1284710:CDS	30.0%
!!	GTTAAGTTTGTTTCTGTTCA+AGG	-	chr8_3:27374870-27374889	Msa1284710:intron	30.0%
	AAAACCAATAAACTTGAACC+AGG	+	chr8_3:27375005-27375024	None:intergenic	30.0%
	GGTAGACAAAAATACTCTAT+TGG	+	chr8_3:27375143-27375162	None:intergenic	30.0%
	CATGACTAAAGATGTTAGAA+AGG	+	chr8_3:27375164-27375183	None:intergenic	30.0%
!	ACAAAGGCTCTTTAAGAAAA+AGG	+	chr8_3:27375242-27375261	None:intergenic	30.0%
	TTAACTGTCATCGAGTAATA+TGG	+	chr8_3:27375288-27375307	None:intergenic	30.0%
	TAAATGGTCATATACATGAG+AGG	+	chr8_3:27375421-27375440	None:intergenic	30.0%
!!	AAAGTGAGAAGGTTATTTCT+AGG	+	chr8_3:27375449-27375468	None:intergenic	30.0%
	GAAATAACCTTCTCACTTTA+CGG	-	chr8_3:27375450-27375469	Msa1284710:intron	30.0%
	ATACCGTAAAGTGATTAAGA+AGG	+	chr8_3:27375492-27375511	None:intergenic	30.0%
	TTAACTCACTCTTACAAAAC+CGG	-	chr8_3:27375548-27375567	Msa1284710:intron	30.0%
	TGGAAATTCAACCTTCATTA+TGG	+	chr8_3:27375712-27375731	None:intergenic	30.0%
	ATATATACTGCAATGGAAGT+AGG	+	chr8_3:27375779-27375798	None:intergenic	30.0%
	CATAATAAGGCTTCAAAGTT+TGG	+	chr8_3:27375811-27375830	None:intergenic	30.0%
!!	ACCATGTAGTTCTTTTGAAA+CGG	-	chr8_3:27376025-27376044	Msa1284710:intron	30.0%
!!!	TTACTTTTTGGAACACCAAT+TGG	+	chr8_3:27376279-27376298	None:intergenic	30.0%
	TACTTCTTTAAACAACACAG+TGG	-	chr8_3:27376346-27376365	Msa1284710:CDS	30.0%
	CATCCAACAGAAGAATAAAA+AGG	-	chr8_3:27376470-27376489	Msa1284710:CDS	30.0%
!	TAGCCTTTTTATTCTTCTGT+TGG	+	chr8_3:27376476-27376495	None:intergenic	30.0%
	ACAGAAGAATAAAAAGGCTA+TGG	-	chr8_3:27376476-27376495	Msa1284710:CDS	30.0%
!	AATTTGCTTACAATTCCAAC+AGG	+	chr8_3:27376537-27376556	None:intergenic	30.0%
	GTAACTTTGAACATGAAATG+TGG	-	chr8_3:27376562-27376581	Msa1284710:five_prime_UTR	30.0%
	TCATGAAATGAAAGTGACTA+AGG	+	chr8_3:27376615-27376634	None:intergenic	30.0%
!	AGTCACTTTCATTTCATGAT+TGG	-	chr8_3:27376616-27376635	Msa1284710:five_prime_UTR	30.0%
!	AACCTGATTTTACCTTCAAA+AGG	-	chr8_3:27376674-27376693	Msa1284710:five_prime_UTR	30.0%
!!	ATCCTTTTGAAGGTAAAATC+AGG	+	chr8_3:27376679-27376698	None:intergenic	30.0%
!!	GGCAAAGTAGCTTTAATAAC+TGG	-	chr8_3:27371921-27371940	Msa1284710:three_prime_UTR	35.0%
	AAAAGTAAAATTGAGGACGG+AGG	+	chr8_3:27372286-27372305	None:intergenic	35.0%
	ACTAACGAAAAACAGCTTTG+AGG	+	chr8_3:27372463-27372482	None:intergenic	35.0%
	GTTGTTAAAGAGTCTCACAT+CGG	-	chr8_3:27372527-27372546	Msa1284710:intron	35.0%
	CATTTCTTAGGAATCGAGAT+TGG	+	chr8_3:27372615-27372634	None:intergenic	35.0%
	TGCAATTTCGATGCATTTAG+GGG	-	chr8_3:27372639-27372658	Msa1284710:intron	35.0%
	GGATACTAAAGACACTATGT+GGG	+	chr8_3:27372677-27372696	None:intergenic	35.0%
	AGGATACTAAAGACACTATG+TGG	+	chr8_3:27372678-27372697	None:intergenic	35.0%
!	TTGGTTAATAGATTTTGGGC+TGG	-	chr8_3:27372766-27372785	Msa1284710:intron	35.0%
!!!	TAATAGATTTTGGGCTGGTT+TGG	-	chr8_3:27372771-27372790	Msa1284710:intron	35.0%
	CAATAGCATAGTTCAATGAG+TGG	+	chr8_3:27373222-27373241	None:intergenic	35.0%
	TTGTCTGTGAAATGTCTCAA+AGG	+	chr8_3:27373418-27373437	None:intergenic	35.0%
	GGTCATTGCAACATTATAGA+AGG	+	chr8_3:27373474-27373493	None:intergenic	35.0%
!	ACTACTCATCTGAAACACAA+TGG	+	chr8_3:27373495-27373514	None:intergenic	35.0%
	GCACTTAAGTATCTCAAGAA+AGG	-	chr8_3:27373668-27373687	Msa1284710:intron	35.0%
	ATTAGTGCTACCTTGCATTA+TGG	-	chr8_3:27373734-27373753	Msa1284710:intron	35.0%
	AATTCATGTATCTGCAGCAA+AGG	-	chr8_3:27373772-27373791	Msa1284710:intron	35.0%
	TGATTCCACTACAAGAAACT+TGG	-	chr8_3:27374018-27374037	Msa1284710:intron	35.0%
	GAAATGCCTCTGTATAAACT+TGG	-	chr8_3:27374160-27374179	Msa1284710:intron	35.0%
	AATGCCTCTGTATAAACTTG+GGG	-	chr8_3:27374162-27374181	Msa1284710:intron	35.0%
	TGTATAAACTTGGGGAGAAA+TGG	-	chr8_3:27374170-27374189	Msa1284710:intron	35.0%
	GTATAAACTTGGGGAGAAAT+GGG	-	chr8_3:27374171-27374190	Msa1284710:intron	35.0%
	CTTCCTTCAAAGTCAATTTG+TGG	-	chr8_3:27374347-27374366	Msa1284710:intron	35.0%
!	TCACCACAAATTGACTTTGA+AGG	+	chr8_3:27374353-27374372	None:intergenic	35.0%
	TTCAAAGTCAATTTGTGGTG+AGG	-	chr8_3:27374352-27374371	Msa1284710:intron	35.0%
!!	CTACTTCTCATTGACTTTGA+AGG	+	chr8_3:27374415-27374434	None:intergenic	35.0%
	AATAAGTAGTGTTTGGTTGG+GGG	-	chr8_3:27374454-27374473	Msa1284710:intron	35.0%
	AGATGTAGTAAGTATGAGCA+GGG	+	chr8_3:27374980-27374999	None:intergenic	35.0%
!!	GAATGAAGAGTATTGCAAGA+TGG	+	chr8_3:27375121-27375140	None:intergenic	35.0%
!!	ATAGAAGTGCTAATGACATG+TGG	+	chr8_3:27375192-27375211	None:intergenic	35.0%
!	AGAAAAAGGTTTTCACGAGA+TGG	+	chr8_3:27375228-27375247	None:intergenic	35.0%
	AAACACTGCATGAATTGACA+AGG	+	chr8_3:27375386-27375405	None:intergenic	35.0%
	ACAAATACCGTAAAGTGAGA+AGG	+	chr8_3:27375460-27375479	None:intergenic	35.0%
!!	GCTCTACCCAAATTTTAAGA+AGG	+	chr8_3:27375528-27375547	None:intergenic	35.0%
	TTCATTGTGTTGGGTTTGAA+GGG	+	chr8_3:27375651-27375670	None:intergenic	35.0%
	TCAGGACATATATACTGCAA+TGG	+	chr8_3:27375786-27375805	None:intergenic	35.0%
	AAGATGGCAAAGATGAAACA+AGG	+	chr8_3:27375923-27375942	None:intergenic	35.0%
	TCCGTTTCAAAAGAACTACA+TGG	+	chr8_3:27376029-27376048	None:intergenic	35.0%
	AGATCCAGAAATGAACCAAT+TGG	-	chr8_3:27376261-27376280	Msa1284710:CDS	35.0%
!	AACACCAATTGGTTCATTTC+TGG	+	chr8_3:27376268-27376287	None:intergenic	35.0%
	TAGTACAAACACACAACCAT+GGG	+	chr8_3:27376508-27376527	None:intergenic	35.0%
	ATAGTACAAACACACAACCA+TGG	+	chr8_3:27376509-27376528	None:intergenic	35.0%
	TTTGTACTATCTTTGCCTGT+TGG	-	chr8_3:27376519-27376538	Msa1284710:exon	35.0%
!	GGTTTATGCTATCCTTTTGA+AGG	+	chr8_3:27376689-27376708	None:intergenic	35.0%
	GTAAAAGACTCCACACACAT+TGG	-	chr8_3:27371737-27371756	Msa1284710:three_prime_UTR	40.0%
	GAATTGAATCAACGCAGTAG+CGG	-	chr8_3:27371835-27371854	Msa1284710:three_prime_UTR	40.0%
!	ATCTGGTTTGAAGGGTGATT+CGG	+	chr8_3:27371892-27371911	None:intergenic	40.0%
	GAAGCAAGTTCTTCAATCTG+CGG	-	chr8_3:27372022-27372041	Msa1284710:three_prime_UTR	40.0%
	AGTTCTTCAATCTGCGGTTT+CGG	-	chr8_3:27372028-27372047	Msa1284710:three_prime_UTR	40.0%
!	TGGCTTTTAAGCATTTTGGG+AGG	-	chr8_3:27372132-27372151	Msa1284710:CDS	40.0%
	ATTCTTGTTAATGCTGCTGC+TGG	-	chr8_3:27372161-27372180	Msa1284710:CDS	40.0%
	TTCTTGTTAATGCTGCTGCT+GGG	-	chr8_3:27372162-27372181	Msa1284710:CDS	40.0%
	TGACTCACGTGTAAATGAAG+TGG	-	chr8_3:27372738-27372757	Msa1284710:intron	40.0%
	TGTAAATGAAGTGGCAGCTT+TGG	-	chr8_3:27372747-27372766	Msa1284710:intron	40.0%
	TATCCAAGCAGGGTCATATT+AGG	-	chr8_3:27373029-27373048	Msa1284710:intron	40.0%
	ATGCTATTGCTAACCACATG+CGG	-	chr8_3:27373233-27373252	Msa1284710:intron	40.0%
!!	GTGTTGGATATCGATTCTGT+TGG	-	chr8_3:27373623-27373642	Msa1284710:intron	40.0%
!	AAGAAACTTGGCACTGGAAT+GGG	-	chr8_3:27374030-27374049	Msa1284710:intron	40.0%
!	AAACTGGCTCCTGAAGAAAT+AGG	-	chr8_3:27374121-27374140	Msa1284710:intron	40.0%
	TTCTCCCCAAGTTTATACAG+AGG	+	chr8_3:27374169-27374188	None:intergenic	40.0%
	GGAGAAATGGGATATTGCAA+TGG	-	chr8_3:27374183-27374202	Msa1284710:CDS	40.0%
!!!	GGTGTTTTGATTCCATGCTT+GGG	-	chr8_3:27374475-27374494	Msa1284710:intron	40.0%
	TGACAGGTAGAACTGAAAAG+AGG	+	chr8_3:27374717-27374736	None:intergenic	40.0%
	AGTCACAGAATAAGACGACA+AGG	+	chr8_3:27374948-27374967	None:intergenic	40.0%
	GAGATGTAGTAAGTATGAGC+AGG	+	chr8_3:27374981-27375000	None:intergenic	40.0%
	CATACTTACTACATCTCACC+TGG	-	chr8_3:27374984-27375003	Msa1284710:intron	40.0%
!	CTCACCTGGTTCAAGTTTAT+TGG	-	chr8_3:27374998-27375017	Msa1284710:intron	40.0%
	AAAGTCTGTAGCAGGATTAC+AGG	+	chr8_3:27375048-27375067	None:intergenic	40.0%
	AGCATTTGAAAGTCTGTAGC+AGG	+	chr8_3:27375056-27375075	None:intergenic	40.0%
	ACTGTCATCGAGTAATATGG+TGG	+	chr8_3:27375285-27375304	None:intergenic	40.0%
	ATGAGTCGAGAGAAGTAAAG+AGG	+	chr8_3:27375321-27375340	None:intergenic	40.0%
	TCTTACAAAACCGGCTGATA+AGG	-	chr8_3:27375557-27375576	Msa1284710:intron	40.0%
	ACTACATAGGCCATATCTCT+AGG	-	chr8_3:27375605-27375624	Msa1284710:intron	40.0%
	ACTCAGAATGCCTAGAGATA+TGG	+	chr8_3:27375618-27375637	None:intergenic	40.0%
	TCATTGTGTTGGGTTTGAAG+GGG	+	chr8_3:27375650-27375669	None:intergenic	40.0%
	CTTCATTGTGTTGGGTTTGA+AGG	+	chr8_3:27375652-27375671	None:intergenic	40.0%
	CTTCAAACCCAACACAATGA+AGG	-	chr8_3:27375650-27375669	Msa1284710:intron	40.0%
!	CTAGGGGTATACCATAATGA+AGG	-	chr8_3:27375698-27375717	Msa1284710:intron	40.0%
	AGGCTTCAAAGTTTGGTATC+AGG	+	chr8_3:27375804-27375823	None:intergenic	40.0%
!	GTCACTGTTTGAGATACCTA+GGG	+	chr8_3:27376063-27376082	None:intergenic	40.0%
!	AGTCACTGTTTGAGATACCT+AGG	+	chr8_3:27376064-27376083	None:intergenic	40.0%
!!	GACACTATGATAGTTGATGG+AGG	-	chr8_3:27376168-27376187	Msa1284710:intron	40.0%
!!	TGATAGTTGATGGAGGACTT+TGG	-	chr8_3:27376175-27376194	Msa1284710:intron	40.0%
!!	CAGGCTCATAGCTTACTTTT+TGG	+	chr8_3:27376291-27376310	None:intergenic	40.0%
	AGTACAAACACACAACCATG+GGG	+	chr8_3:27376507-27376526	None:intergenic	40.0%
	TGAACATGAAATGTGGCTAG+TGG	-	chr8_3:27376569-27376588	Msa1284710:five_prime_UTR	40.0%
	GTTTCAGACACCAATGTGTG+TGG	+	chr8_3:27371750-27371769	None:intergenic	45.0%
!	GCGAATTTAGCGTCGAAACA+TGG	-	chr8_3:27371857-27371876	Msa1284710:three_prime_UTR	45.0%
	TTTAATAACTGGCGGAGCGT+CGG	-	chr8_3:27371932-27371951	Msa1284710:three_prime_UTR	45.0%
	TCAACGCAATTGGGGAAACA+TGG	-	chr8_3:27371972-27371991	Msa1284710:three_prime_UTR	45.0%
	ATTCCAGCTGTTGGGTTTGA+AGG	-	chr8_3:27372068-27372087	Msa1284710:three_prime_UTR	45.0%
	TTCCAGCTGTTGGGTTTGAA+GGG	-	chr8_3:27372069-27372088	Msa1284710:three_prime_UTR	45.0%
!	GAATTTTCTTGCTGCTGCTG+AGG	-	chr8_3:27372184-27372203	Msa1284710:CDS	45.0%
	TCACCTGTTCGAAACCCATT+CGG	+	chr8_3:27372218-27372237	None:intergenic	45.0%
	AGTGTCTATAGGTCACATGC+CGG	-	chr8_3:27372569-27372588	Msa1284710:intron	45.0%
	TCTAACACAGCCCAAGAAAC+CGG	+	chr8_3:27372591-27372610	None:intergenic	45.0%
!!!	GATTTTGGGCTGGTTTGGAT+TGG	-	chr8_3:27372776-27372795	Msa1284710:intron	45.0%
	ACACCTAATATGACCCTGCT+TGG	+	chr8_3:27373035-27373054	None:intergenic	45.0%
	TGCTATTGCTAACCACATGC+GGG	-	chr8_3:27373234-27373253	Msa1284710:intron	45.0%
	CCAAGAAGCTCCATAATGCA+AGG	+	chr8_3:27373747-27373766	None:intergenic	45.0%
	CCTTGCATTATGGAGCTTCT+TGG	-	chr8_3:27373744-27373763	Msa1284710:intron	45.0%
	CTACATCACTCTACTCTCGT+TGG	-	chr8_3:27373912-27373931	Msa1284710:intron	45.0%
	CAGTGCCAAGTTTCTTGTAG+TGG	+	chr8_3:27374026-27374045	None:intergenic	45.0%
	CACTACAAGAAACTTGGCAC+TGG	-	chr8_3:27374024-27374043	Msa1284710:intron	45.0%
!	CAAGAAACTTGGCACTGGAA+TGG	-	chr8_3:27374029-27374048	Msa1284710:intron	45.0%
!	AGAAACTTGGCACTGGAATG+GGG	-	chr8_3:27374031-27374050	Msa1284710:intron	45.0%
!!	ATGCCAGGAGTGTCACTTAT+TGG	+	chr8_3:27374097-27374116	None:intergenic	45.0%
!	CCTCTGCTACCTATTTCTTC+AGG	+	chr8_3:27374133-27374152	None:intergenic	45.0%
	CCTGAAGAAATAGGTAGCAG+AGG	-	chr8_3:27374130-27374149	Msa1284710:intron	45.0%
	CTCTACTTAGCATCAGATGC+AGG	-	chr8_3:27374211-27374230	Msa1284710:CDS	45.0%
!!!	GGGTGTTTTGATTCCATGCT+TGG	-	chr8_3:27374474-27374493	Msa1284710:intron	45.0%
!	TCCATGCTTGGGTTTTGCTT+TGG	-	chr8_3:27374486-27374505	Msa1284710:intron	45.0%
	ACCAAAGCAAAACCCAAGCA+TGG	+	chr8_3:27374490-27374509	None:intergenic	45.0%
!!	TTGGTGACAGTCTAGCTTTC+TGG	-	chr8_3:27374505-27374524	Msa1284710:intron	45.0%
	GTAGAACTGAAAAGAGGCCA+AGG	+	chr8_3:27374711-27374730	None:intergenic	45.0%
	TGGTGGCAGCTATTAAAGAG+TGG	+	chr8_3:27375268-27375287	None:intergenic	45.0%
	CTGCATGAATTGACAAGGAG+AGG	+	chr8_3:27375381-27375400	None:intergenic	45.0%
	AAAACCGGCTGATAAGGTGA+GGG	-	chr8_3:27375563-27375582	Msa1284710:intron	45.0%
	TGGGTTGCCTTCATTGTGTT+GGG	+	chr8_3:27375660-27375679	None:intergenic	45.0%
!	TTGGGAAGATCTAGAAGTGC+TGG	+	chr8_3:27375732-27375751	None:intergenic	45.0%
!	ACGGATGCGTTTTTAACCCT+AGG	-	chr8_3:27376044-27376063	Msa1284710:intron	45.0%
	GGCGACACTATGATAGTTGA+TGG	-	chr8_3:27376165-27376184	Msa1284710:intron	45.0%
	TAAGCTATGAGCCTGCAGAT+TGG	-	chr8_3:27376296-27376315	Msa1284710:CDS	45.0%
	AAGCTATGAGCCTGCAGATT+GGG	-	chr8_3:27376297-27376316	Msa1284710:CDS	45.0%
	AGTAATCCCTGCAGTGTAGA+GGG	+	chr8_3:27376330-27376349	None:intergenic	45.0%
	AAGTAATCCCTGCAGTGTAG+AGG	+	chr8_3:27376331-27376350	None:intergenic	45.0%
!!!	AATTTTATCTTTTTAATTTA+AGG	-	chr8_3:27373820-27373839	Msa1284710:intron	5.0%
!!	AAATCAATAAAAAAATTAAA+GGG	+	chr8_3:27376094-27376113	None:intergenic	5.0%
!	TTAATAACTGGCGGAGCGTC+GGG	-	chr8_3:27371933-27371952	Msa1284710:three_prime_UTR	50.0%
	ATTGGGGAAACATGGAGCGT+CGG	-	chr8_3:27371980-27371999	Msa1284710:three_prime_UTR	50.0%
	TCGCTTTCGATTCCAGCTGT+TGG	-	chr8_3:27372059-27372078	Msa1284710:three_prime_UTR	50.0%
	CGCTTTCGATTCCAGCTGTT+GGG	-	chr8_3:27372060-27372079	Msa1284710:three_prime_UTR	50.0%
	TCCAGCTGTTGGGTTTGAAG+GGG	-	chr8_3:27372070-27372089	Msa1284710:three_prime_UTR	50.0%
	TTGGGTTTGAAGGGGATGTG+AGG	-	chr8_3:27372078-27372097	Msa1284710:three_prime_UTR	50.0%
	TGTGAGGAAGCAGGAAGATG+CGG	-	chr8_3:27372094-27372113	Msa1284710:exon	50.0%
!	TGCGGCGAGAGTTGTTGATT+TGG	-	chr8_3:27372112-27372131	Msa1284710:CDS	50.0%
	CTGAGGATCTTTCGCCGAAT+GGG	-	chr8_3:27372201-27372220	Msa1284710:CDS	50.0%
!	GAATGGGTTTCGAACAGGTG+AGG	-	chr8_3:27372217-27372236	Msa1284710:CDS	50.0%
!!	TAGGTCACATGCCGGTTTCT+TGG	-	chr8_3:27372577-27372596	Msa1284710:intron	50.0%
!!	AGGTCACATGCCGGTTTCTT+GGG	-	chr8_3:27372578-27372597	Msa1284710:intron	50.0%
!!	TATCTCAAGAAAGGGGCACC+TGG	-	chr8_3:27373677-27373696	Msa1284710:intron	50.0%
	CCTGGTAGGAACTCTTCTAG+CGG	-	chr8_3:27373695-27373714	Msa1284710:intron	50.0%
	CTGGTAGGAACTCTTCTAGC+GGG	-	chr8_3:27373696-27373715	Msa1284710:intron	50.0%
	TGGTAGGAACTCTTCTAGCG+GGG	-	chr8_3:27373697-27373716	Msa1284710:intron	50.0%
!!	GGAGTGTCACTTATTGGACC+TGG	+	chr8_3:27374091-27374110	None:intergenic	50.0%
	GGTCCAATAAGTGACACTCC+TGG	-	chr8_3:27374091-27374110	Msa1284710:intron	50.0%
	CACTCCTGGCATGAGTAAAC+TGG	-	chr8_3:27374105-27374124	Msa1284710:intron	50.0%
	CAAAACCGGCTGATAAGGTG+AGG	-	chr8_3:27375562-27375581	Msa1284710:intron	50.0%
	GTAACCCTCACCTTATCAGC+CGG	+	chr8_3:27375570-27375589	None:intergenic	50.0%
	TTGTGTTGGGTTTGAAGGGG+TGG	+	chr8_3:27375647-27375666	None:intergenic	50.0%
!	GTGGGTTGCCTTCATTGTGT+TGG	+	chr8_3:27375661-27375680	None:intergenic	50.0%
!	GCAACCCACTTGCTGCAATT+AGG	-	chr8_3:27375672-27375691	Msa1284710:intron	50.0%
!	ACCCACTTGCTGCAATTAGG+CGG	-	chr8_3:27375675-27375694	Msa1284710:intron	50.0%
	TTGCTGCAATTAGGCGGCTA+GGG	-	chr8_3:27375681-27375700	Msa1284710:intron	50.0%
	TATTAGCCCCTCTACACTGC+AGG	-	chr8_3:27376320-27376339	Msa1284710:CDS	50.0%
	ATTAGCCCCTCTACACTGCA+GGG	-	chr8_3:27376321-27376340	Msa1284710:CDS	50.0%
	GTAATCCCTGCAGTGTAGAG+GGG	+	chr8_3:27376329-27376348	None:intergenic	50.0%
	TCCCCTTCAAACCCAACAGC+TGG	+	chr8_3:27372074-27372093	None:intergenic	55.0%
	TGAAGGGGATGTGAGGAAGC+AGG	-	chr8_3:27372085-27372104	Msa1284710:three_prime_UTR	55.0%
	GCTGAGGATCTTTCGCCGAA+TGG	-	chr8_3:27372200-27372219	Msa1284710:CDS	55.0%
!	TCGCCGAATGGGTTTCGAAC+AGG	-	chr8_3:27372212-27372231	Msa1284710:CDS	55.0%
!!	TCAAGAAAGGGGCACCTGGT+AGG	-	chr8_3:27373681-27373700	Msa1284710:intron	55.0%
	CCGCTAGAAGAGTTCCTACC+AGG	+	chr8_3:27373698-27373717	None:intergenic	55.0%
	GGTAGGAACTCTTCTAGCGG+GGG	-	chr8_3:27373698-27373717	Msa1284710:intron	55.0%
	CGAGTCAATGGGATTGCACC+AGG	-	chr8_3:27374070-27374089	Msa1284710:intron	55.0%
	GGAGCCAGTTTACTCATGCC+AGG	+	chr8_3:27374112-27374131	None:intergenic	55.0%
	GCCGCCTAATTGCAGCAAGT+GGG	+	chr8_3:27375679-27375698	None:intergenic	55.0%
	AGCCGCCTAATTGCAGCAAG+TGG	+	chr8_3:27375680-27375699	None:intergenic	55.0%
	CTTGCTGCAATTAGGCGGCT+AGG	-	chr8_3:27375680-27375699	Msa1284710:intron	55.0%
	TGCTGCAATTAGGCGGCTAG+GGG	-	chr8_3:27375682-27375701	Msa1284710:intron	55.0%
	CCTTCGGCAAATAACGTGGC+CGG	+	chr8_3:27376206-27376225	None:intergenic	55.0%
	GCCACGTTATTTGCCGAAGG+AGG	-	chr8_3:27376206-27376225	Msa1284710:CDS	55.0%
	GCCTCCTTCGGCAAATAACG+TGG	+	chr8_3:27376210-27376229	None:intergenic	55.0%
	ACCTGTCTCACTGCCTCCTT+CGG	+	chr8_3:27376222-27376241	None:intergenic	55.0%
	GGGGCTAATACCCAATCTGC+AGG	+	chr8_3:27376310-27376329	None:intergenic	55.0%
	AAGGCTATGGCTAGACCCCA+TGG	-	chr8_3:27376489-27376508	Msa1284710:CDS	55.0%
!!	TGGAGCGTCGGTTGCTGTGA+TGG	-	chr8_3:27371992-27372011	Msa1284710:three_prime_UTR	60.0%
!!	GGAGCGTCGGTTGCTGTGAT+GGG	-	chr8_3:27371993-27372012	Msa1284710:three_prime_UTR	60.0%
!!	CGGTTGCTGTGATGGGTCGT+CGG	-	chr8_3:27372000-27372019	Msa1284710:three_prime_UTR	60.0%
	GGTTAACACGGTCCCGCATG+TGG	+	chr8_3:27373249-27373268	None:intergenic	60.0%
	CCGGCCACGTTATTTGCCGA+AGG	-	chr8_3:27376203-27376222	Msa1284710:CDS	60.0%
!!	ACTGGCGGAGCGTCGGGAAT+TGG	-	chr8_3:27371939-27371958	Msa1284710:three_prime_UTR	65.0%
!!	CTGGCGGAGCGTCGGGAATT+GGG	-	chr8_3:27371940-27371959	Msa1284710:three_prime_UTR	65.0%
	GCCGAAGGAGGCAGTGAGAC+AGG	-	chr8_3:27376218-27376237	Msa1284710:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	27371703	27376708	27371703	ID=Msa1284710;Name=Msa1284710
chr8_3	mRNA	27371703	27376708	27371703	ID=Msa1284710-mRNA-1;Parent=Msa1284710;Name=Msa1284710-mRNA-1;_AED=0.05;_eAED=0.05;_QI=173\|0.66\|0.5\|1\|1\|1\|4\|403\|253
chr8_3	exon	27371703	27372260	27371703	ID=Msa1284710-mRNA-1:exon:9422;Parent=Msa1284710-mRNA-1
chr8_3	exon	27374179	27374352	27374179	ID=Msa1284710-mRNA-1:exon:9421;Parent=Msa1284710-mRNA-1
chr8_3	exon	27374713	27374787	27374713	ID=Msa1284710-mRNA-1:exon:9420;Parent=Msa1284710-mRNA-1
chr8_3	exon	27376178	27376708	27376178	ID=Msa1284710-mRNA-1:exon:9419;Parent=Msa1284710-mRNA-1
chr8_3	five_prime_UTR	27376536	27376708	27376536	ID=Msa1284710-mRNA-1:five_prime_utr;Parent=Msa1284710-mRNA-1
chr8_3	CDS	27376178	27376535	27376178	ID=Msa1284710-mRNA-1:cds;Parent=Msa1284710-mRNA-1
chr8_3	CDS	27374713	27374787	27374713	ID=Msa1284710-mRNA-1:cds;Parent=Msa1284710-mRNA-1
chr8_3	CDS	27374179	27374352	27374179	ID=Msa1284710-mRNA-1:cds;Parent=Msa1284710-mRNA-1
chr8_3	CDS	27372106	27372260	27372106	ID=Msa1284710-mRNA-1:cds;Parent=Msa1284710-mRNA-1
chr8_3	three_prime_UTR	27371703	27372105	27371703	ID=Msa1284710-mRNA-1:three_prime_utr;Parent=Msa1284710-mRNA-1

Gene Sequence

>Msa1284710

ATGGTAGCTTCAACCGAATCACCCTTCAAACCAGATATCTTAAAAGGCAAAGTAGCTTTAATAACTGGCGGAGCGTCGGGAATTGGGTTTGAAATATCAACGCAATTGGGGAAACATGGAGCGTCGGTTGCTGTGATGGGTCGTCGGAAGCAAGTTCTTCAATCTGCGGTTTCGGTTCTTCAATCGCTTTCGATTCCAGCTGTTGGGTTTGAAGGGGATGTGAGGAAGCAGGAAGATGCGGCGAGAGTTGTTGATTTGGCTTTTAAGCATTTTGGGAGGATTGATATTCTTGTTAATGCTGCTGCTGGGAATTTTCTTGCTGCTGCTGAGGATCTTTCGCCGAATGGGTTTCGAACAGTGTTGGATATCGATTCTGTTGGCACATTCACTATGTGTCATGAAGCACTTAAGTATCTCAAGAAAGGGGCACCTGATTATGATATTCGAGTCAATGGGATTGCACCAGGTCCAATAAGTGACACTCCTGGCATGAGTAAACTGGCTCCTGAAGAAATAGGTAGCAGAGGCAGAGATGAAATGCCTCTGTATAAACTTGGGGAGAAATGGGATATTGCAATGGCTGCTCTCTACTTAGCATCAGATGCAGGAAAATACATTAATGGCGACACTATGATAGTTGATGGAGGACTTTGGCTGAGCCGGCCACGTTATTTGCCGAAGGAGGCAGTGAGACAGGTATCGCGAGAAGTAGAAAAAAGATCCAGAAATGAACCAATTGGTGTTCCAAAAAGTAAGCTATGA

Protein sequence

>Msa1284710

MVASTESPFKPDILKGKVALITGGASGIGFEISTQLGKHGASVAVMGRRKQVLQSAVSVLQSLSIPAVGFEGDVRKQEDAARVVDLAFKHFGRIDILVNAAAGNFLAAAEDLSPNGFRTVLDIDSVGTFTMCHEALKYLKKGAPDYDIRVNGIAPGPISDTPGMSKLAPEEIGSRGRDEMPLYKLGEKWDIAMAALYLASDAGKYINGDTMIVDGGLWLSRPRYLPKEAVRQVSREVEKRSRNEPIGVPKSKL*