AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0698930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0698930 MtrunA17_Chr4g0052371 86.992 123 4 4 1 122 1 112 1.50e-56 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCAGCAGACGATTATTT+TGG 0.182988 4_4:+69389171 Msa0698930:intron
TCCATTGTCCGGAACTCTTT+TGG 0.290169 4_4:+69388866 Msa0698930:CDS
TCAGGATTTAATTGAAATCA+AGG 0.374856 4_4:+69389062 Msa0698930:CDS
TGCTGCAAAGCAGGCAGCCA+AGG 0.380640 4_4:+69389237 Msa0698930:CDS
TGAATTTGCCAAAAGAGTTC+CGG 0.381401 4_4:-69388874 None:intergenic
TCCCCTTCCGATTGGGTAGC+CGG 0.386098 4_4:-69388838 None:intergenic
CTTCCGAACTTTAGTTGCCT+TGG 0.396128 4_4:-69389254 None:intergenic
GAGTAAAGCTGCTGCAAAGC+AGG 0.435629 4_4:+69389228 Msa0698930:CDS
ATTAAATCCTGAGGAATGTT+GGG 0.440220 4_4:-69389051 None:intergenic
AATTAAATCCTGAGGAATGT+TGG 0.444643 4_4:-69389052 None:intergenic
CAAGCTTATGATTTCCTCAC+TGG 0.467518 4_4:+69389011 Msa0698930:CDS
GATTTACTCCCCTTCCGATT+GGG 0.479142 4_4:-69388845 None:intergenic
ACGTACGCCGGCTACCCAAT+CGG 0.500846 4_4:+69388831 Msa0698930:CDS
GGATTTACTCCCCTTCCGAT+TGG 0.503941 4_4:-69388846 None:intergenic
CAGCCAAGGCAACTAAAGTT+CGG 0.506043 4_4:+69389251 Msa0698930:CDS
GCAGAAGGGGAAGAAAGTGT+TGG 0.512031 4_4:+69389324 Msa0698930:CDS
GAAGGAGCCCAACATTCCTC+AGG 0.521672 4_4:+69389044 Msa0698930:CDS
ATTGGGTAGCCGGCGTACGT+TGG 0.539138 4_4:-69388828 None:intergenic
GCCAAAAGAGTTCCGGACAA+TGG 0.540316 4_4:-69388867 None:intergenic
AGTAAAATTCATAAGCAGAA+GGG 0.542068 4_4:+69389310 Msa0698930:CDS
AGGGGAGTAAATCCATTGTC+CGG 0.546668 4_4:+69388855 Msa0698930:CDS
GCCGGCTACCCAATCGGAAG+GGG 0.548832 4_4:+69388837 Msa0698930:CDS
GTAAAATTCATAAGCAGAAG+GGG 0.557860 4_4:+69389311 Msa0698930:CDS
AAGTAAAATTCATAAGCAGA+AGG 0.561096 4_4:+69389309 Msa0698930:CDS
TTGATTTCAATTAAATCCTG+AGG 0.568823 4_4:-69389060 None:intergenic
ACGCCGGCTACCCAATCGGA+AGG 0.586765 4_4:+69388835 Msa0698930:CDS
TACTCTGTCGATATTCAGCA+AGG 0.588341 4_4:-69389210 None:intergenic
CGCCGGCTACCCAATCGGAA+GGG 0.588497 4_4:+69388836 Msa0698930:CDS
AAGCAAAATCCAACGTACGC+CGG 0.639196 4_4:+69388819 Msa0698930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGATTTCAATTAAATCCTG+AGG - chr4_4:69389063-69389082 None:intergenic 25.0%
! TCAGGATTTAATTGAAATCA+AGG + chr4_4:69389062-69389081 Msa0698930:CDS 25.0%
! AAGTAAAATTCATAAGCAGA+AGG + chr4_4:69389309-69389328 Msa0698930:CDS 25.0%
! AGTAAAATTCATAAGCAGAA+GGG + chr4_4:69389310-69389329 Msa0698930:CDS 25.0%
! AACTCTTTTGGCAAATTCAA+AGG + chr4_4:69388878-69388897 Msa0698930:CDS 30.0%
! ATTCAAAGGCAAGTTTTTCT+CGG + chr4_4:69388892-69388911 Msa0698930:intron 30.0%
ATATCACTCTTTATACCAAC+AGG + chr4_4:69388969-69388988 Msa0698930:intron 30.0%
ATTAAATCCTGAGGAATGTT+GGG - chr4_4:69389054-69389073 None:intergenic 30.0%
AATTAAATCCTGAGGAATGT+TGG - chr4_4:69389055-69389074 None:intergenic 30.0%
GTAAAATTCATAAGCAGAAG+GGG + chr4_4:69389311-69389330 Msa0698930:CDS 30.0%
TGAATTTGCCAAAAGAGTTC+CGG - chr4_4:69388877-69388896 None:intergenic 35.0%
! TTCAAAGGCAAGTTTTTCTC+GGG + chr4_4:69388893-69388912 Msa0698930:intron 35.0%
! AAACCAGCAGACGATTATTT+TGG + chr4_4:69389171-69389190 Msa0698930:intron 35.0%
AGAGAATCAGAAGCAAGCAT+GGG - chr4_4:69388925-69388944 None:intergenic 40.0%
GTGACAATTCGTAATCCTGT+TGG - chr4_4:69388987-69389006 None:intergenic 40.0%
CAAGCTTATGATTTCCTCAC+TGG + chr4_4:69389011-69389030 Msa0698930:CDS 40.0%
! CTTCAACTCTTTTTCCAGTG+AGG - chr4_4:69389028-69389047 None:intergenic 40.0%
CTCACTGGAAAAAGAGTTGA+AGG + chr4_4:69389026-69389045 Msa0698930:CDS 40.0%
TACTCTGTCGATATTCAGCA+AGG - chr4_4:69389213-69389232 None:intergenic 40.0%
AAGCAAAATCCAACGTACGC+CGG + chr4_4:69388819-69388838 Msa0698930:CDS 45.0%
GATTTACTCCCCTTCCGATT+GGG - chr4_4:69388848-69388867 None:intergenic 45.0%
AGGGGAGTAAATCCATTGTC+CGG + chr4_4:69388855-69388874 Msa0698930:CDS 45.0%
! TCCATTGTCCGGAACTCTTT+TGG + chr4_4:69388866-69388885 Msa0698930:CDS 45.0%
CAGAGAATCAGAAGCAAGCA+TGG - chr4_4:69388926-69388945 None:intergenic 45.0%
CCTCCAAAATAATCGTCTGC+TGG - chr4_4:69389177-69389196 None:intergenic 45.0%
!!! CCAGCAGACGATTATTTTGG+AGG + chr4_4:69389174-69389193 Msa0698930:intron 45.0%
CAGCCAAGGCAACTAAAGTT+CGG + chr4_4:69389251-69389270 Msa0698930:CDS 45.0%
CTTCCGAACTTTAGTTGCCT+TGG - chr4_4:69389257-69389276 None:intergenic 45.0%
GGATTTACTCCCCTTCCGAT+TGG - chr4_4:69388849-69388868 None:intergenic 50.0%
GCCAAAAGAGTTCCGGACAA+TGG - chr4_4:69388870-69388889 None:intergenic 50.0%
TCAGAAGCAAGCATGGGTAC+AGG - chr4_4:69388919-69388938 None:intergenic 50.0%
GAGTAAAGCTGCTGCAAAGC+AGG + chr4_4:69389228-69389247 Msa0698930:CDS 50.0%
! GCAGAAGGGGAAGAAAGTGT+TGG + chr4_4:69389324-69389343 Msa0698930:CDS 50.0%
GAAGGAGCCCAACATTCCTC+AGG + chr4_4:69389044-69389063 Msa0698930:CDS 55.0%
ATTGGGTAGCCGGCGTACGT+TGG - chr4_4:69388831-69388850 None:intergenic 60.0%
! ACGTACGCCGGCTACCCAAT+CGG + chr4_4:69388831-69388850 Msa0698930:CDS 60.0%
TCCCCTTCCGATTGGGTAGC+CGG - chr4_4:69388841-69388860 None:intergenic 60.0%
TGCTGCAAAGCAGGCAGCCA+AGG + chr4_4:69389237-69389256 Msa0698930:CDS 60.0%
! ACGCCGGCTACCCAATCGGA+AGG + chr4_4:69388835-69388854 Msa0698930:CDS 65.0%
! CGCCGGCTACCCAATCGGAA+GGG + chr4_4:69388836-69388855 Msa0698930:CDS 65.0%
GCCGGCTACCCAATCGGAAG+GGG + chr4_4:69388837-69388856 Msa0698930:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 69388801 69389354 69388801 ID=Msa0698930;Name=Msa0698930
chr4_4 mRNA 69388801 69389354 69388801 ID=Msa0698930-mRNA-1;Parent=Msa0698930;Name=Msa0698930-mRNA-1;_AED=0.39;_eAED=0.42;_QI=0|0|0|1|1|1|3|0|122
chr4_4 exon 69388801 69388899 69388801 ID=Msa0698930-mRNA-1:exon:20805;Parent=Msa0698930-mRNA-1
chr4_4 exon 69388991 69389083 69388991 ID=Msa0698930-mRNA-1:exon:20806;Parent=Msa0698930-mRNA-1
chr4_4 exon 69389178 69389354 69389178 ID=Msa0698930-mRNA-1:exon:20807;Parent=Msa0698930-mRNA-1
chr4_4 CDS 69388801 69388899 69388801 ID=Msa0698930-mRNA-1:cds;Parent=Msa0698930-mRNA-1
chr4_4 CDS 69388991 69389083 69388991 ID=Msa0698930-mRNA-1:cds;Parent=Msa0698930-mRNA-1
chr4_4 CDS 69389178 69389354 69389178 ID=Msa0698930-mRNA-1:cds;Parent=Msa0698930-mRNA-1
Gene Sequence

>Msa0698930

ATGGTTTATTGTAGTGGAAAGCAAAATCCAACGTACGCCGGCTACCCAATCGGAAGGGGAGTAAATCCATTGTCCGGAACTCTTTTGGCAAATTCAAAGGATTACGAATTGTCACAATACAAGCTTATGATTTCCTCACTGGAAAAAGAGTTGAAGGAGCCCAACATTCCTCAGGATTTAATTGAAATCAAGCAGACGATTATTTTGGAGGATTGTCGTGTTCTCCTTGCTGAATATCGACAGAGTAAAGCTGCTGCAAAGCAGGCAGCCAAGGCAACTAAAGTTCGGAAGACAAGTAAAATTCAAAAGAAGACAGCTAAGACAAGTAAAATTCATAAGCAGAAGGGGAAGAAAGTGTTGGAGACTTGA

Protein sequence

>Msa0698930

MVYCSGKQNPTYAGYPIGRGVNPLSGTLLANSKDYELSQYKLMISSLEKELKEPNIPQDLIEIKQTIILEDCRVLLAEYRQSKAAAKQAAKATKVRKTSKIQKKTAKTSKIHKQKGKKVLET*