Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06223.t1 | QDX15124.1 | 89 | 181 | 20 | 0 | 37 | 217 | 35 | 215 | 5.40E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06223.t1 | Q9SMP0 | 77.9 | 190 | 31 | 5 | 37 | 217 | 46 | 233 | 2.9e-69 | 263.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06223.t1 | A0A5B8HMF8 | 89.0 | 181 | 20 | 0 | 37 | 217 | 35 | 215 | 3.9e-83 | 317.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene06223.t1 | TF | NF-YC |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene06223 | 0.813264 | 2.79E-51 | -1.69E-46 |
| MS.gene049171 | MS.gene06223 | 0.816748 | 4.71E-52 | -1.69E-46 |
| MS.gene049173 | MS.gene06223 | 0.835821 | 1.34E-56 | -1.69E-46 |
| MS.gene049217 | MS.gene06223 | 0.880232 | 6.50E-70 | -1.69E-46 |
| MS.gene049222 | MS.gene06223 | 0.856397 | 3.31E-62 | -1.69E-46 |
| MS.gene049285 | MS.gene06223 | 0.881737 | 1.87E-70 | -1.69E-46 |
| MS.gene049320 | MS.gene06223 | 0.885743 | 6.25E-72 | -1.69E-46 |
| MS.gene04939 | MS.gene06223 | 0.809115 | 2.22E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene06223 | 0.848321 | 6.60E-60 | -1.69E-46 |
| MS.gene049609 | MS.gene06223 | 0.8308 | 2.39E-55 | -1.69E-46 |
| MS.gene049614 | MS.gene06223 | 0.881709 | 1.92E-70 | -1.69E-46 |
| MS.gene049627 | MS.gene06223 | 0.809233 | 2.09E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene06223 | 0.814155 | 1.78E-51 | -1.69E-46 |
| MS.gene049760 | MS.gene06223 | 0.809537 | 1.80E-50 | -1.69E-46 |
| MS.gene049805 | MS.gene06223 | 0.842507 | 2.48E-58 | -1.69E-46 |
| MS.gene049828 | MS.gene06223 | 0.84701 | 1.52E-59 | -1.69E-46 |
| MS.gene050076 | MS.gene06223 | 0.829186 | 5.93E-55 | -1.69E-46 |
| MS.gene050083 | MS.gene06223 | -0.807893 | 4.05E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene06223 | 0.929259 | 8.98E-93 | -1.69E-46 |
| MS.gene050165 | MS.gene06223 | 0.916944 | 9.67E-86 | -1.69E-46 |
| MS.gene050333 | MS.gene06223 | 0.885049 | 1.14E-71 | -1.69E-46 |
| MS.gene050353 | MS.gene06223 | -0.839646 | 1.40E-57 | -1.69E-46 |
| MS.gene050432 | MS.gene06223 | 0.810361 | 1.20E-50 | -1.69E-46 |
| MS.gene050444 | MS.gene06223 | 0.817578 | 3.07E-52 | -1.69E-46 |
| MS.gene050475 | MS.gene06223 | -0.811943 | 5.44E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene06223 | 0.85719 | 1.93E-62 | -1.69E-46 |
| MS.gene050518 | MS.gene06223 | 0.850453 | 1.68E-60 | -1.69E-46 |
| MS.gene050541 | MS.gene06223 | -0.805914 | 1.06E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene06223 | 0.851526 | 8.38E-61 | -1.69E-46 |
| MS.gene050675 | MS.gene06223 | 0.834963 | 2.21E-56 | -1.69E-46 |
| MS.gene050699 | MS.gene06223 | 0.847858 | 8.86E-60 | -1.69E-46 |
| MS.gene050747 | MS.gene06223 | 0.803411 | 3.53E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene06223 | 0.910461 | 1.83E-82 | -1.69E-46 |
| MS.gene050770 | MS.gene06223 | 0.914638 | 1.52E-84 | -1.69E-46 |
| MS.gene050772 | MS.gene06223 | 0.859787 | 3.25E-63 | -1.69E-46 |
| MS.gene05079 | MS.gene06223 | -0.812386 | 4.35E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene06223 | 0.803337 | 3.66E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene06223 | 0.834038 | 3.78E-56 | -1.69E-46 |
| MS.gene05100 | MS.gene06223 | 0.898421 | 5.39E-77 | -1.69E-46 |
| MS.gene05101 | MS.gene06223 | 0.821407 | 4.11E-53 | -1.69E-46 |
| MS.gene051228 | MS.gene06223 | 0.874676 | 5.61E-68 | -1.69E-46 |
| MS.gene051551 | MS.gene06223 | 0.815996 | 6.94E-52 | -1.69E-46 |
| MS.gene051564 | MS.gene06223 | 0.812026 | 5.21E-51 | -1.69E-46 |
| MS.gene051566 | MS.gene06223 | 0.813837 | 2.09E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene06223 | 0.831376 | 1.73E-55 | -1.69E-46 |
| MS.gene051692 | MS.gene06223 | 0.801172 | 1.02E-48 | -1.69E-46 |
| MS.gene051694 | MS.gene06223 | 0.840216 | 9.94E-58 | -1.69E-46 |
| MS.gene051810 | MS.gene06223 | 0.908378 | 1.82E-81 | -1.69E-46 |
| MS.gene051858 | MS.gene06223 | 0.858271 | 9.24E-63 | -1.69E-46 |
| MS.gene051949 | MS.gene06223 | 0.857033 | 2.15E-62 | -1.69E-46 |
| MS.gene052078 | MS.gene06223 | 0.800034 | 1.74E-48 | -1.69E-46 |
| MS.gene052271 | MS.gene06223 | 0.90684 | 9.65E-81 | -1.69E-46 |
| MS.gene052290 | MS.gene06223 | 0.890685 | 7.88E-74 | -1.69E-46 |
| MS.gene052326 | MS.gene06223 | 0.816874 | 4.42E-52 | -1.69E-46 |
| MS.gene052379 | MS.gene06223 | 0.897251 | 1.68E-76 | -1.69E-46 |
| MS.gene052421 | MS.gene06223 | 0.804305 | 2.30E-49 | -1.69E-46 |
| MS.gene052484 | MS.gene06223 | 0.807105 | 5.95E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene06223 | 0.839431 | 1.59E-57 | -1.69E-46 |
| MS.gene052670 | MS.gene06223 | 0.835493 | 1.63E-56 | -1.69E-46 |
| MS.gene052916 | MS.gene06223 | 0.837659 | 4.56E-57 | -1.69E-46 |
| MS.gene053009 | MS.gene06223 | 0.880127 | 7.09E-70 | -1.69E-46 |
| MS.gene053103 | MS.gene06223 | 0.811493 | 6.81E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene06223 | 0.826802 | 2.22E-54 | -1.69E-46 |
| MS.gene053207 | MS.gene06223 | 0.831757 | 1.39E-55 | -1.69E-46 |
| MS.gene053213 | MS.gene06223 | 0.884673 | 1.57E-71 | -1.69E-46 |
| MS.gene053286 | MS.gene06223 | 0.875795 | 2.32E-68 | -1.69E-46 |
| MS.gene053322 | MS.gene06223 | 0.912449 | 1.92E-83 | -1.69E-46 |
| MS.gene053574 | MS.gene06223 | 0.818438 | 1.96E-52 | -1.69E-46 |
| MS.gene053632 | MS.gene06223 | 0.878017 | 3.95E-69 | -1.69E-46 |
| MS.gene053915 | MS.gene06223 | 0.81572 | 8.00E-52 | -1.69E-46 |
| MS.gene053916 | MS.gene06223 | 0.818527 | 1.87E-52 | -1.69E-46 |
| MS.gene054376 | MS.gene06223 | 0.88974 | 1.85E-73 | -1.69E-46 |
| MS.gene054408 | MS.gene06223 | 0.800345 | 1.51E-48 | -1.69E-46 |
| MS.gene054600 | MS.gene06223 | 0.850353 | 1.79E-60 | -1.69E-46 |
| MS.gene054642 | MS.gene06223 | 0.903143 | 4.70E-79 | -1.69E-46 |
| MS.gene054802 | MS.gene06223 | 0.887098 | 1.92E-72 | -1.69E-46 |
| MS.gene054803 | MS.gene06223 | 0.887752 | 1.08E-72 | -1.69E-46 |
| MS.gene054804 | MS.gene06223 | 0.890863 | 6.70E-74 | -1.69E-46 |
| MS.gene054954 | MS.gene06223 | 0.840894 | 6.60E-58 | -1.69E-46 |
| MS.gene05505 | MS.gene06223 | 0.838804 | 2.31E-57 | -1.69E-46 |
| MS.gene055154 | MS.gene06223 | -0.812302 | 4.54E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene06223 | 0.820395 | 7.02E-53 | -1.69E-46 |
| MS.gene055212 | MS.gene06223 | 0.843363 | 1.47E-58 | -1.69E-46 |
| MS.gene055254 | MS.gene06223 | 0.892697 | 1.25E-74 | -1.69E-46 |
| MS.gene055289 | MS.gene06223 | 0.80259 | 5.22E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene06223 | 0.870723 | 1.18E-66 | -1.69E-46 |
| MS.gene055397 | MS.gene06223 | 0.920503 | 1.18E-87 | -1.69E-46 |
| MS.gene055412 | MS.gene06223 | 0.823621 | 1.26E-53 | -1.69E-46 |
| MS.gene055499 | MS.gene06223 | 0.96743 | 2.88E-127 | -1.69E-46 |
| MS.gene055502 | MS.gene06223 | 0.85312 | 2.95E-61 | -1.69E-46 |
| MS.gene055577 | MS.gene06223 | 0.819744 | 9.89E-53 | -1.69E-46 |
| MS.gene055636 | MS.gene06223 | 0.835456 | 1.66E-56 | -1.69E-46 |
| MS.gene055638 | MS.gene06223 | 0.851064 | 1.13E-60 | -1.69E-46 |
| MS.gene055647 | MS.gene06223 | 0.844795 | 6.06E-59 | -1.69E-46 |
| MS.gene055722 | MS.gene06223 | 0.838287 | 3.15E-57 | -1.69E-46 |
| MS.gene05592 | MS.gene06223 | 0.870775 | 1.13E-66 | -1.69E-46 |
| MS.gene055941 | MS.gene06223 | 0.827087 | 1.90E-54 | -1.69E-46 |
| MS.gene055956 | MS.gene06223 | -0.841786 | 3.85E-58 | -1.69E-46 |
| MS.gene056083 | MS.gene06223 | 0.855525 | 5.95E-62 | -1.69E-46 |
| MS.gene056374 | MS.gene06223 | 0.872954 | 2.14E-67 | -1.69E-46 |
| MS.gene056410 | MS.gene06223 | 0.806816 | 6.84E-50 | -1.69E-46 |
| MS.gene056449 | MS.gene06223 | 0.812612 | 3.88E-51 | -1.69E-46 |
| MS.gene056549 | MS.gene06223 | 0.82382 | 1.13E-53 | -1.69E-46 |
| MS.gene056609 | MS.gene06223 | 0.832589 | 8.67E-56 | -1.69E-46 |
| MS.gene056611 | MS.gene06223 | 0.806861 | 6.70E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene06223 | 0.884481 | 1.85E-71 | -1.69E-46 |
| MS.gene056692 | MS.gene06223 | 0.823071 | 1.69E-53 | -1.69E-46 |
| MS.gene056897 | MS.gene06223 | 0.816287 | 5.98E-52 | -1.69E-46 |
| MS.gene056903 | MS.gene06223 | 0.804061 | 2.59E-49 | -1.69E-46 |
| MS.gene057011 | MS.gene06223 | 0.829006 | 6.55E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene06223 | 0.808985 | 2.37E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene06223 | 0.938228 | 9.52E-99 | -1.69E-46 |
| MS.gene057146 | MS.gene06223 | 0.835457 | 1.66E-56 | -1.69E-46 |
| MS.gene057246 | MS.gene06223 | 0.85817 | 9.90E-63 | -1.69E-46 |
| MS.gene057295 | MS.gene06223 | 0.841787 | 3.84E-58 | -1.69E-46 |
| MS.gene057331 | MS.gene06223 | 0.815083 | 1.11E-51 | -1.69E-46 |
| MS.gene05735 | MS.gene06223 | 0.808702 | 2.72E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene06223 | 0.865298 | 6.54E-65 | -1.69E-46 |
| MS.gene057926 | MS.gene06223 | 0.826152 | 3.17E-54 | -1.69E-46 |
| MS.gene058006 | MS.gene06223 | 0.810615 | 1.06E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene06223 | 0.807202 | 5.67E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene06223 | 0.812313 | 4.51E-51 | -1.69E-46 |
| MS.gene058323 | MS.gene06223 | 0.857262 | 1.84E-62 | -1.69E-46 |
| MS.gene058419 | MS.gene06223 | 0.876166 | 1.73E-68 | -1.69E-46 |
| MS.gene058544 | MS.gene06223 | 0.832608 | 8.58E-56 | -1.69E-46 |
| MS.gene058660 | MS.gene06223 | 0.838526 | 2.73E-57 | -1.69E-46 |
| MS.gene058723 | MS.gene06223 | 0.873654 | 1.24E-67 | -1.69E-46 |
| MS.gene058773 | MS.gene06223 | 0.871218 | 8.08E-67 | -1.69E-46 |
| MS.gene058840 | MS.gene06223 | -0.809221 | 2.11E-50 | -1.69E-46 |
| MS.gene058903 | MS.gene06223 | 0.806604 | 7.59E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene06223 | 0.871789 | 5.23E-67 | -1.69E-46 |
| MS.gene059023 | MS.gene06223 | 0.803712 | 3.06E-49 | -1.69E-46 |
| MS.gene059029 | MS.gene06223 | 0.883733 | 3.49E-71 | -1.69E-46 |
| MS.gene059102 | MS.gene06223 | -0.829488 | 5.00E-55 | -1.69E-46 |
| MS.gene05913 | MS.gene06223 | 0.809248 | 2.08E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene06223 | 0.833763 | 4.42E-56 | -1.69E-46 |
| MS.gene059536 | MS.gene06223 | 0.875307 | 3.42E-68 | -1.69E-46 |
| MS.gene059677 | MS.gene06223 | 0.900241 | 8.92E-78 | -1.69E-46 |
| MS.gene059718 | MS.gene06223 | 0.863591 | 2.23E-64 | -1.69E-46 |
| MS.gene059858 | MS.gene06223 | 0.854112 | 1.53E-61 | -1.69E-46 |
| MS.gene059865 | MS.gene06223 | 0.802402 | 5.71E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene06223 | 0.860476 | 2.01E-63 | -1.69E-46 |
| MS.gene059960 | MS.gene06223 | 0.89583 | 6.60E-76 | -1.69E-46 |
| MS.gene060337 | MS.gene06223 | -0.804979 | 1.67E-49 | -1.69E-46 |
| MS.gene060341 | MS.gene06223 | 0.811584 | 6.51E-51 | -1.69E-46 |
| MS.gene060356 | MS.gene06223 | 0.806574 | 7.70E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene06223 | 0.818075 | 2.37E-52 | -1.69E-46 |
| MS.gene060728 | MS.gene06223 | 0.801178 | 1.02E-48 | -1.69E-46 |
| MS.gene060762 | MS.gene06223 | 0.837229 | 5.88E-57 | -1.69E-46 |
| MS.gene06077 | MS.gene06223 | 0.816022 | 6.85E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene06223 | 0.841118 | 5.77E-58 | -1.69E-46 |
| MS.gene060818 | MS.gene06223 | 0.8384 | 2.94E-57 | -1.69E-46 |
| MS.gene06092 | MS.gene06223 | 0.825219 | 5.28E-54 | -1.69E-46 |
| MS.gene061041 | MS.gene06223 | 0.846333 | 2.32E-59 | -1.69E-46 |
| MS.gene061134 | MS.gene06223 | 0.834083 | 3.68E-56 | -1.69E-46 |
| MS.gene061150 | MS.gene06223 | 0.865622 | 5.17E-65 | -1.69E-46 |
| MS.gene061171 | MS.gene06223 | 0.82985 | 4.09E-55 | -1.69E-46 |
| MS.gene061200 | MS.gene06223 | 0.91463 | 1.53E-84 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06223.t1 | MTR_3g099180 | 98.165 | 218 | 1 | 3 | 1 | 217 | 1 | 216 | 5.29e-156 | 431 |
| MS.gene06223.t1 | MTR_3g099180 | 98.165 | 218 | 1 | 3 | 1 | 217 | 1 | 216 | 5.57e-156 | 431 |
| MS.gene06223.t1 | MTR_7g113680 | 66.038 | 159 | 40 | 4 | 38 | 185 | 134 | 289 | 2.46e-65 | 204 |
| MS.gene06223.t1 | MTR_1g082660 | 69.014 | 142 | 33 | 2 | 38 | 168 | 83 | 224 | 5.31e-63 | 197 |
| MS.gene06223.t1 | MTR_2g023340 | 48.889 | 180 | 72 | 4 | 38 | 199 | 67 | 244 | 3.48e-49 | 161 |
| MS.gene06223.t1 | MTR_2g081600 | 62.069 | 87 | 32 | 1 | 55 | 140 | 21 | 107 | 6.06e-32 | 113 |
| MS.gene06223.t1 | MTR_2g081630 | 61.364 | 88 | 33 | 1 | 55 | 141 | 21 | 108 | 1.85e-31 | 112 |
| MS.gene06223.t1 | MTR_3g012030 | 40.506 | 79 | 47 | 0 | 56 | 134 | 8 | 86 | 1.26e-11 | 63.2 |
| MS.gene06223.t1 | MTR_8g041420 | 37.975 | 79 | 49 | 0 | 56 | 134 | 8 | 86 | 1.87e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06223.t1 | AT3G48590 | 86.339 | 183 | 18 | 3 | 24 | 200 | 33 | 214 | 5.20e-102 | 295 |
| MS.gene06223.t1 | AT5G63470 | 76.887 | 212 | 25 | 4 | 12 | 200 | 20 | 230 | 1.13e-101 | 295 |
| MS.gene06223.t1 | AT5G63470 | 76.887 | 212 | 25 | 4 | 12 | 200 | 20 | 230 | 1.13e-101 | 295 |
| MS.gene06223.t1 | AT1G56170 | 70.588 | 136 | 35 | 2 | 35 | 168 | 55 | 187 | 1.25e-65 | 201 |
| MS.gene06223.t1 | AT1G56170 | 70.588 | 136 | 35 | 2 | 35 | 168 | 55 | 187 | 1.25e-65 | 201 |
| MS.gene06223.t1 | AT1G54830 | 63.522 | 159 | 48 | 4 | 38 | 190 | 52 | 206 | 1.79e-64 | 199 |
| MS.gene06223.t1 | AT1G54830 | 63.522 | 159 | 48 | 4 | 38 | 190 | 52 | 206 | 1.79e-64 | 199 |
| MS.gene06223.t1 | AT1G54830 | 63.522 | 159 | 48 | 4 | 38 | 190 | 52 | 206 | 1.79e-64 | 199 |
| MS.gene06223.t1 | AT1G08970 | 67.586 | 145 | 37 | 3 | 38 | 173 | 62 | 205 | 2.85e-63 | 197 |
| MS.gene06223.t1 | AT1G08970 | 67.586 | 145 | 37 | 3 | 38 | 173 | 62 | 205 | 2.85e-63 | 197 |
| MS.gene06223.t1 | AT1G08970 | 67.586 | 145 | 37 | 3 | 38 | 173 | 62 | 205 | 2.85e-63 | 197 |
| MS.gene06223.t1 | AT1G08970 | 67.586 | 145 | 37 | 3 | 38 | 173 | 62 | 205 | 2.85e-63 | 197 |
| MS.gene06223.t1 | AT5G50480 | 51.323 | 189 | 67 | 8 | 21 | 200 | 20 | 192 | 8.65e-53 | 169 |
| MS.gene06223.t1 | AT5G27910 | 43.779 | 217 | 88 | 7 | 1 | 213 | 1 | 187 | 3.40e-46 | 152 |
| MS.gene06223.t1 | AT5G50490 | 45.946 | 148 | 72 | 3 | 30 | 174 | 13 | 155 | 4.40e-37 | 128 |
| MS.gene06223.t1 | AT5G50470 | 39.086 | 197 | 94 | 7 | 21 | 208 | 31 | 210 | 7.58e-35 | 123 |
| MS.gene06223.t1 | AT5G38140 | 44.000 | 100 | 52 | 2 | 35 | 133 | 48 | 144 | 1.54e-25 | 99.0 |
| MS.gene06223.t1 | AT3G12480 | 37.975 | 79 | 49 | 0 | 56 | 134 | 8 | 86 | 1.60e-11 | 62.8 |
Find 55 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAGGCTTGGCAGTCTGTT+TGG | 0.288988 | 3.1:+79057319 | MS.gene06223:CDS |
| TTGAACTCACCATTCGTTCT+TGG | 0.290380 | 3.1:+79057037 | MS.gene06223:CDS |
| TCCTTATTATTATCCTCCTA+TGG | 0.322109 | 3.1:+79057224 | MS.gene06223:CDS |
| CCCATAGGAGGATAATAATA+AGG | 0.322822 | 3.1:-79057225 | None:intergenic |
| GGGTTGGATATTGTTGTTGC+TGG | 0.331351 | 3.1:-79056780 | None:intergenic |
| GGTGTAACTGCCGTGGGTGA+AGG | 0.360117 | 3.1:-79056802 | None:intergenic |
| CAAGCTTTGGCAAAGAGAAT+TGG | 0.395526 | 3.1:-79057000 | None:intergenic |
| GTGTAACTGCCGTGGGTGAA+GGG | 0.405293 | 3.1:-79056801 | None:intergenic |
| TTATGTTCAGCCGCCTTCTC+AGG | 0.407879 | 3.1:+79057302 | MS.gene06223:CDS |
| AATGAAAAGCTCACAAGCTT+TGG | 0.414215 | 3.1:-79057013 | None:intergenic |
| TGGTCGTCCCGCTGTTGATC+CGG | 0.430949 | 3.1:+79057269 | MS.gene06223:CDS |
| CCTCCTATGGGACAACCTGC+TGG | 0.443243 | 3.1:+79057237 | MS.gene06223:CDS |
| TTCAGCCGCCTTCTCAGGCT+TGG | 0.445274 | 3.1:+79057307 | MS.gene06223:CDS |
| ATATTGCTGCTGCTATTACT+AGG | 0.451638 | 3.1:+79057103 | MS.gene06223:CDS |
| TGGCAGACGGGAGCGGATGA+TGG | 0.454235 | 3.1:+79057339 | MS.gene06223:CDS |
| AACAACAGCTTCAAATGTTC+TGG | 0.455902 | 3.1:+79056857 | MS.gene06223:CDS |
| TGGCAGTCTGTTTGGCAGAC+GGG | 0.457754 | 3.1:+79057327 | MS.gene06223:CDS |
| TCTTATGCTGGTGGTGGTGT+TGG | 0.460055 | 3.1:+79057363 | MS.gene06223:CDS |
| TTGGCAGTCTGTTTGGCAGA+CGG | 0.463047 | 3.1:+79057326 | MS.gene06223:CDS |
| GTCTGTTTGGCAGACGGGAG+CGG | 0.463208 | 3.1:+79057332 | MS.gene06223:CDS |
| CCCGCTGTTGATCCGGCTAC+CGG | 0.469158 | 3.1:+79057276 | MS.gene06223:CDS |
| CCTTATTATTATCCTCCTAT+GGG | 0.475192 | 3.1:+79057225 | MS.gene06223:CDS |
| GCGGATGATGGTTCTTATGC+TGG | 0.494268 | 3.1:+79057351 | MS.gene06223:CDS |
| GTTGTTGTTGGAGAAGATGT+TGG | 0.512349 | 3.1:-79056834 | None:intergenic |
| ATCGAACAAAATGATACCTC+TGG | 0.517463 | 3.1:-79057410 | None:intergenic |
| ATTCCAGCAGGTTGTCCCAT+AGG | 0.521379 | 3.1:-79057240 | None:intergenic |
| GCTGGTGGTGGTGTTGGCAG+TGG | 0.530864 | 3.1:+79057369 | MS.gene06223:CDS |
| CAACCTGCTGGAATGATGAT+TGG | 0.531059 | 3.1:+79057249 | MS.gene06223:CDS |
| TTCAATTTCTTGACGTTGGT+AGG | 0.532955 | 3.1:-79056881 | None:intergenic |
| TCATCTTTAATCTCGTCTCT+CGG | 0.544717 | 3.1:-79057159 | None:intergenic |
| TGAACATAAACACCGGTAGC+CGG | 0.556443 | 3.1:-79057288 | None:intergenic |
| ATTGTTGGTGCTGCTGCTAG+TGG | 0.559151 | 3.1:+79057198 | MS.gene06223:CDS |
| CGACCAATCATCATTCCAGC+AGG | 0.560161 | 3.1:-79057252 | None:intergenic |
| TGCGTGCTAATGGAAGTTGA+TGG | 0.566195 | 3.1:-79056924 | None:intergenic |
| CCGGTAGCCGGATCAACAGC+GGG | 0.566961 | 3.1:-79057276 | None:intergenic |
| AGTGGTCAGCACAATGGCGA+TGG | 0.568390 | 3.1:+79057387 | MS.gene06223:CDS |
| TGGTGGTGGTGTAACTGCCG+TGG | 0.571103 | 3.1:-79056809 | None:intergenic |
| CGTGTTCAATTTCTTGACGT+TGG | 0.574575 | 3.1:-79056885 | None:intergenic |
| GATGGTTCTTATGCTGGTGG+TGG | 0.575597 | 3.1:+79057357 | MS.gene06223:CDS |
| ACTGCCGTGGGTGAAGGGGT+TGG | 0.575732 | 3.1:-79056796 | None:intergenic |
| TGGAGAAGATGTTGGAATGG+TGG | 0.580552 | 3.1:-79056826 | None:intergenic |
| GACTGCCAAGCCTGAGAAGG+CGG | 0.581694 | 3.1:-79057312 | None:intergenic |
| AGCATGAAGCCAAGAACGAA+TGG | 0.590029 | 3.1:-79057046 | None:intergenic |
| TGTTGGAGAAGATGTTGGAA+TGG | 0.596521 | 3.1:-79056829 | None:intergenic |
| TGTAACTGCCGTGGGTGAAG+GGG | 0.596946 | 3.1:-79056800 | None:intergenic |
| CCAGCAGGTTGTCCCATAGG+AGG | 0.597127 | 3.1:-79057237 | None:intergenic |
| ACAGACTGCCAAGCCTGAGA+AGG | 0.599594 | 3.1:-79057315 | None:intergenic |
| AGCACAATGGCGATGGCCAG+AGG | 0.601116 | 3.1:+79057394 | MS.gene06223:CDS |
| AGAAGATGTTGGAATGGTGG+TGG | 0.605173 | 3.1:-79056823 | None:intergenic |
| ATATCCAACCCCTTCACCCA+CGG | 0.620411 | 3.1:+79056792 | MS.gene06223:CDS |
| GGTGGTGGTGTAACTGCCGT+GGG | 0.631253 | 3.1:-79056808 | None:intergenic |
| ACCGGTAGCCGGATCAACAG+CGG | 0.640253 | 3.1:-79057277 | None:intergenic |
| GTTGGCAGTGGTCAGCACAA+TGG | 0.663712 | 3.1:+79057381 | MS.gene06223:CDS |
| GATGATGGTTCTTATGCTGG+TGG | 0.665141 | 3.1:+79057354 | MS.gene06223:CDS |
| AGGCGGCTGAACATAAACAC+CGG | 0.726448 | 3.1:-79057295 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GACTGATATTTTTGATTTTT+TGG | + | chr3.1:79057125-79057144 | MS.gene06223:CDS | 20.0% |
| CCTTATTATTATCCTCCTAT+GGG | + | chr3.1:79057225-79057244 | MS.gene06223:CDS | 30.0% | |
| TCCTTATTATTATCCTCCTA+TGG | + | chr3.1:79057224-79057243 | MS.gene06223:CDS | 30.0% | |
| ! | ATTTTCTTAATGCGTGCTAA+TGG | - | chr3.1:79056937-79056956 | None:intergenic | 30.0% |
| AACAACAGCTTCAAATGTTC+TGG | + | chr3.1:79056857-79056876 | MS.gene06223:CDS | 35.0% | |
| AATGAAAAGCTCACAAGCTT+TGG | - | chr3.1:79057016-79057035 | None:intergenic | 35.0% | |
| ATATTGCTGCTGCTATTACT+AGG | + | chr3.1:79057103-79057122 | MS.gene06223:CDS | 35.0% | |
| ATCGAACAAAATGATACCTC+TGG | - | chr3.1:79057413-79057432 | None:intergenic | 35.0% | |
| CCCATAGGAGGATAATAATA+AGG | - | chr3.1:79057228-79057247 | None:intergenic | 35.0% | |
| TCATCTTTAATCTCGTCTCT+CGG | - | chr3.1:79057162-79057181 | None:intergenic | 35.0% | |
| TTCAATTTCTTGACGTTGGT+AGG | - | chr3.1:79056884-79056903 | None:intergenic | 35.0% | |
| CGTGTTCAATTTCTTGACGT+TGG | - | chr3.1:79056888-79056907 | None:intergenic | 40.0% | |
| TGTTGGAGAAGATGTTGGAA+TGG | - | chr3.1:79056832-79056851 | None:intergenic | 40.0% | |
| TTGAACTCACCATTCGTTCT+TGG | + | chr3.1:79057037-79057056 | MS.gene06223:CDS | 40.0% | |
| ! | CAAGCTTTGGCAAAGAGAAT+TGG | - | chr3.1:79057003-79057022 | None:intergenic | 40.0% |
| ! | GAAGCTGTTGTTGTTGTTGT+TGG | - | chr3.1:79056849-79056868 | None:intergenic | 40.0% |
| ! | GTTGTTGTTGGAGAAGATGT+TGG | - | chr3.1:79056837-79056856 | None:intergenic | 40.0% |
| AGAAGATGTTGGAATGGTGG+TGG | - | chr3.1:79056826-79056845 | None:intergenic | 45.0% | |
| AGCATGAAGCCAAGAACGAA+TGG | - | chr3.1:79057049-79057068 | None:intergenic | 45.0% | |
| CAACCTGCTGGAATGATGAT+TGG | + | chr3.1:79057249-79057268 | MS.gene06223:CDS | 45.0% | |
| TGAACATAAACACCGGTAGC+CGG | - | chr3.1:79057291-79057310 | None:intergenic | 45.0% | |
| TGCGTGCTAATGGAAGTTGA+TGG | - | chr3.1:79056927-79056946 | None:intergenic | 45.0% | |
| TGGAGAAGATGTTGGAATGG+TGG | - | chr3.1:79056829-79056848 | None:intergenic | 45.0% | |
| ! | GATGATGGTTCTTATGCTGG+TGG | + | chr3.1:79057354-79057373 | MS.gene06223:CDS | 45.0% |
| ! | GGGTTGGATATTGTTGTTGC+TGG | - | chr3.1:79056783-79056802 | None:intergenic | 45.0% |
| AGGCGGCTGAACATAAACAC+CGG | - | chr3.1:79057298-79057317 | None:intergenic | 50.0% | |
| ATATCCAACCCCTTCACCCA+CGG | + | chr3.1:79056792-79056811 | MS.gene06223:CDS | 50.0% | |
| ATTCCAGCAGGTTGTCCCAT+AGG | - | chr3.1:79057243-79057262 | None:intergenic | 50.0% | |
| CGACCAATCATCATTCCAGC+AGG | - | chr3.1:79057255-79057274 | None:intergenic | 50.0% | |
| GATGGTTCTTATGCTGGTGG+TGG | + | chr3.1:79057357-79057376 | MS.gene06223:CDS | 50.0% | |
| TTATGTTCAGCCGCCTTCTC+AGG | + | chr3.1:79057302-79057321 | MS.gene06223:CDS | 50.0% | |
| TTGGCAGTCTGTTTGGCAGA+CGG | + | chr3.1:79057326-79057345 | MS.gene06223:CDS | 50.0% | |
| ! | GCGGATGATGGTTCTTATGC+TGG | + | chr3.1:79057351-79057370 | MS.gene06223:CDS | 50.0% |
| !! | ATTGTTGGTGCTGCTGCTAG+TGG | + | chr3.1:79057198-79057217 | MS.gene06223:CDS | 50.0% |
| !! | TCTTATGCTGGTGGTGGTGT+TGG | + | chr3.1:79057363-79057382 | MS.gene06223:CDS | 50.0% |
| ACAGACTGCCAAGCCTGAGA+AGG | - | chr3.1:79057318-79057337 | None:intergenic | 55.0% | |
| AGTGGTCAGCACAATGGCGA+TGG | + | chr3.1:79057387-79057406 | MS.gene06223:CDS | 55.0% | |
| CTCAGGCTTGGCAGTCTGTT+TGG | + | chr3.1:79057319-79057338 | MS.gene06223:CDS | 55.0% | |
| GCTGCTGCTGCTGCTATTGT+TGG | + | chr3.1:79057183-79057202 | MS.gene06223:CDS | 55.0% | |
| GTGTAACTGCCGTGGGTGAA+GGG | - | chr3.1:79056804-79056823 | None:intergenic | 55.0% | |
| GTTGGCAGTGGTCAGCACAA+TGG | + | chr3.1:79057381-79057400 | MS.gene06223:CDS | 55.0% | |
| TGGCAGTCTGTTTGGCAGAC+GGG | + | chr3.1:79057327-79057346 | MS.gene06223:CDS | 55.0% | |
| TGTAACTGCCGTGGGTGAAG+GGG | - | chr3.1:79056803-79056822 | None:intergenic | 55.0% | |
| ACCGGTAGCCGGATCAACAG+CGG | - | chr3.1:79057280-79057299 | None:intergenic | 60.0% | |
| AGCACAATGGCGATGGCCAG+AGG | + | chr3.1:79057394-79057413 | MS.gene06223:CDS | 60.0% | |
| CCAGCAGGTTGTCCCATAGG+AGG | - | chr3.1:79057240-79057259 | None:intergenic | 60.0% | |
| CCTCCTATGGGACAACCTGC+TGG | + | chr3.1:79057237-79057256 | MS.gene06223:CDS | 60.0% | |
| GACTGCCAAGCCTGAGAAGG+CGG | - | chr3.1:79057315-79057334 | None:intergenic | 60.0% | |
| GGTGTAACTGCCGTGGGTGA+AGG | - | chr3.1:79056805-79056824 | None:intergenic | 60.0% | |
| GTCTGTTTGGCAGACGGGAG+CGG | + | chr3.1:79057332-79057351 | MS.gene06223:CDS | 60.0% | |
| TGGTCGTCCCGCTGTTGATC+CGG | + | chr3.1:79057269-79057288 | MS.gene06223:CDS | 60.0% | |
| TTCAGCCGCCTTCTCAGGCT+TGG | + | chr3.1:79057307-79057326 | MS.gene06223:CDS | 60.0% | |
| ! | GGTGGTGGTGTAACTGCCGT+GGG | - | chr3.1:79056811-79056830 | None:intergenic | 60.0% |
| !! | TGGTGGTGGTGTAACTGCCG+TGG | - | chr3.1:79056812-79056831 | None:intergenic | 60.0% |
| ACTGCCGTGGGTGAAGGGGT+TGG | - | chr3.1:79056799-79056818 | None:intergenic | 65.0% | |
| CCCGCTGTTGATCCGGCTAC+CGG | + | chr3.1:79057276-79057295 | MS.gene06223:CDS | 65.0% | |
| CCGGTAGCCGGATCAACAGC+GGG | - | chr3.1:79057279-79057298 | None:intergenic | 65.0% | |
| TGGCAGACGGGAGCGGATGA+TGG | + | chr3.1:79057339-79057358 | MS.gene06223:CDS | 65.0% | |
| !! | GCTGGTGGTGGTGTTGGCAG+TGG | + | chr3.1:79057369-79057388 | MS.gene06223:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 79056763 | 79057440 | 79056763 | ID=MS.gene06223 |
| chr3.1 | mRNA | 79056763 | 79057440 | 79056763 | ID=MS.gene06223.t1;Parent=MS.gene06223 |
| chr3.1 | exon | 79056763 | 79057440 | 79056763 | ID=MS.gene06223.t1.exon1;Parent=MS.gene06223.t1 |
| chr3.1 | CDS | 79056763 | 79057440 | 79056763 | ID=cds.MS.gene06223.t1;Parent=MS.gene06223.t1 |
| Gene Sequence |
| Protein sequence |
