AlfalfaGEDB | MS.gene68738

M. sativa cultivar XinJiangDaYe / MS.gene68738

KEGG: K01845
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006725 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008483 GO:0009058 GO:0009987 GO:0016740 GO:0016769 GO:0016853 GO:0016866 GO:0016869 GO:0018130 GO:0019438 GO:0019842 GO:0030170 GO:0033013 GO:0033014 GO:0034641 GO:0036094 GO:0042286 GO:0043167 GO:0043168 GO:0044237 GO:0044249 GO:0044271 GO:0046483 GO:0048037 GO:0050662 GO:0070279 GO:0071704 GO:0097159 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00202.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68738.t1	AEN94095.1	100	439	0	0	2	440	33	471	9.10E-252	879

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68738.t1	P45621	91.8	440	35	1	1	440	28	466	1.0e-235	817.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68738.t1	G4W9I6	100.0	439	0	0	2	440	33	471	6.5e-252	879.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene68738	0.854458	1.21E-61	-1.69E-46
MS.gene049173	MS.gene68738	0.840133	1.04E-57	-1.69E-46
MS.gene049217	MS.gene68738	0.8679	9.73E-66	-1.69E-46
MS.gene049222	MS.gene68738	0.85864	7.17E-63	-1.69E-46
MS.gene049232	MS.gene68738	0.803758	2.99E-49	-1.69E-46
MS.gene049285	MS.gene68738	0.90937	6.14E-82	-1.69E-46
MS.gene049320	MS.gene68738	0.883889	3.06E-71	-1.69E-46
MS.gene04939	MS.gene68738	0.808306	3.31E-50	-1.69E-46
MS.gene049427	MS.gene68738	0.838452	2.85E-57	-1.69E-46
MS.gene049609	MS.gene68738	0.803611	3.21E-49	-1.69E-46
MS.gene049614	MS.gene68738	0.818769	1.65E-52	-1.69E-46
MS.gene049627	MS.gene68738	0.897736	1.05E-76	-1.69E-46
MS.gene049633	MS.gene68738	0.806172	9.36E-50	-1.69E-46
MS.gene049805	MS.gene68738	0.805229	1.48E-49	-1.69E-46
MS.gene05010	MS.gene68738	0.8226	2.17E-53	-1.69E-46
MS.gene050163	MS.gene68738	0.928147	4.35E-92	-1.69E-46
MS.gene050165	MS.gene68738	0.928119	4.53E-92	-1.69E-46
MS.gene050333	MS.gene68738	0.926803	2.84E-91	-1.69E-46
MS.gene050444	MS.gene68738	0.811142	8.12E-51	-1.69E-46
MS.gene050504	MS.gene68738	0.88407	2.62E-71	-1.69E-46
MS.gene050518	MS.gene68738	0.892194	1.99E-74	-1.69E-46
MS.gene050577	MS.gene68738	0.844733	6.30E-59	-1.69E-46
MS.gene050612	MS.gene68738	0.87584	2.24E-68	-1.69E-46
MS.gene050675	MS.gene68738	0.845745	3.35E-59	-1.69E-46
MS.gene050699	MS.gene68738	0.864879	8.85E-65	-1.69E-46
MS.gene050747	MS.gene68738	0.811701	6.14E-51	-1.69E-46
MS.gene050767	MS.gene68738	0.836932	7.00E-57	-1.69E-46
MS.gene050770	MS.gene68738	0.839027	2.02E-57	-1.69E-46
MS.gene050772	MS.gene68738	0.854282	1.37E-61	-1.69E-46
MS.gene050781	MS.gene68738	0.812904	3.35E-51	-1.69E-46
MS.gene050876	MS.gene68738	0.865425	5.96E-65	-1.69E-46
MS.gene05087	MS.gene68738	0.879978	8.01E-70	-1.69E-46
MS.gene050904	MS.gene68738	0.828468	8.84E-55	-1.69E-46
MS.gene050950	MS.gene68738	0.814969	1.17E-51	-1.69E-46
MS.gene050986	MS.gene68738	0.874489	6.49E-68	-1.69E-46
MS.gene05100	MS.gene68738	0.898517	4.91E-77	-1.69E-46
MS.gene051228	MS.gene68738	0.816473	5.43E-52	-1.69E-46
MS.gene051317	MS.gene68738	0.839159	1.87E-57	-1.69E-46
MS.gene051434	MS.gene68738	0.810444	1.15E-50	-1.69E-46
MS.gene051551	MS.gene68738	0.827619	1.42E-54	-1.69E-46
MS.gene051694	MS.gene68738	0.832839	7.52E-56	-1.69E-46
MS.gene051810	MS.gene68738	0.872067	4.23E-67	-1.69E-46
MS.gene051858	MS.gene68738	0.818736	1.68E-52	-1.69E-46
MS.gene051949	MS.gene68738	0.852015	6.09E-61	-1.69E-46
MS.gene052271	MS.gene68738	0.865436	5.91E-65	-1.69E-46
MS.gene052379	MS.gene68738	0.849479	3.15E-60	-1.69E-46
MS.gene052482	MS.gene68738	0.831323	1.78E-55	-1.69E-46
MS.gene052486	MS.gene68738	0.846658	1.89E-59	-1.69E-46
MS.gene052605	MS.gene68738	0.802521	5.39E-49	-1.69E-46
MS.gene052615	MS.gene68738	0.801928	7.14E-49	-1.69E-46
MS.gene052670	MS.gene68738	0.837509	4.98E-57	-1.69E-46
MS.gene052872	MS.gene68738	0.800023	1.75E-48	-1.69E-46
MS.gene052916	MS.gene68738	0.82414	9.49E-54	-1.69E-46
MS.gene053203	MS.gene68738	0.803537	3.32E-49	-1.69E-46
MS.gene053207	MS.gene68738	0.809178	2.15E-50	-1.69E-46
MS.gene053213	MS.gene68738	0.872846	2.32E-67	-1.69E-46
MS.gene053284	MS.gene68738	0.804798	1.82E-49	-1.69E-46
MS.gene053285	MS.gene68738	0.802109	6.56E-49	-1.69E-46
MS.gene053286	MS.gene68738	0.872532	2.96E-67	-1.69E-46
MS.gene053322	MS.gene68738	0.849911	2.39E-60	-1.69E-46
MS.gene053574	MS.gene68738	0.809453	1.88E-50	-1.69E-46
MS.gene053632	MS.gene68738	0.824422	8.14E-54	-1.69E-46
MS.gene054374	MS.gene68738	0.841943	3.50E-58	-1.69E-46
MS.gene054376	MS.gene68738	0.807408	5.13E-50	-1.69E-46
MS.gene054408	MS.gene68738	0.81576	7.84E-52	-1.69E-46
MS.gene054595	MS.gene68738	0.803818	2.91E-49	-1.69E-46
MS.gene054600	MS.gene68738	0.84823	7.00E-60	-1.69E-46
MS.gene054638	MS.gene68738	0.821415	4.09E-53	-1.69E-46
MS.gene054642	MS.gene68738	0.895997	5.63E-76	-1.69E-46
MS.gene05470	MS.gene68738	0.815924	7.20E-52	-1.69E-46
MS.gene054802	MS.gene68738	0.830166	3.42E-55	-1.69E-46
MS.gene054803	MS.gene68738	0.83088	2.29E-55	-1.69E-46
MS.gene054804	MS.gene68738	0.833094	6.50E-56	-1.69E-46
MS.gene054954	MS.gene68738	0.886101	4.58E-72	-1.69E-46
MS.gene055206	MS.gene68738	0.802018	6.85E-49	-1.69E-46
MS.gene055333	MS.gene68738	-0.814624	1.40E-51	-1.69E-46
MS.gene055362	MS.gene68738	0.887212	1.74E-72	-1.69E-46
MS.gene055397	MS.gene68738	0.857422	1.65E-62	-1.69E-46
MS.gene055499	MS.gene68738	0.856343	3.43E-62	-1.69E-46
MS.gene055502	MS.gene68738	0.865164	7.20E-65	-1.69E-46
MS.gene055577	MS.gene68738	0.820994	5.11E-53	-1.69E-46
MS.gene055634	MS.gene68738	0.876151	1.75E-68	-1.69E-46
MS.gene055636	MS.gene68738	0.868828	4.89E-66	-1.69E-46
MS.gene055638	MS.gene68738	0.883155	5.70E-71	-1.69E-46
MS.gene055647	MS.gene68738	0.844766	6.17E-59	-1.69E-46
MS.gene055722	MS.gene68738	0.820898	5.38E-53	-1.69E-46
MS.gene055748	MS.gene68738	0.841453	4.71E-58	-1.69E-46
MS.gene055750	MS.gene68738	0.832849	7.48E-56	-1.69E-46
MS.gene055751	MS.gene68738	0.848671	5.28E-60	-1.69E-46
MS.gene055941	MS.gene68738	0.815457	9.15E-52	-1.69E-46
MS.gene055956	MS.gene68738	-0.802008	6.88E-49	-1.69E-46
MS.gene056083	MS.gene68738	0.837201	5.98E-57	-1.69E-46
MS.gene056374	MS.gene68738	0.866273	3.22E-65	-1.69E-46
MS.gene056409	MS.gene68738	0.825141	5.51E-54	-1.69E-46
MS.gene056449	MS.gene68738	0.835769	1.38E-56	-1.69E-46
MS.gene056450	MS.gene68738	0.826093	3.28E-54	-1.69E-46
MS.gene056504	MS.gene68738	0.823184	1.59E-53	-1.69E-46
MS.gene056510	MS.gene68738	0.850369	1.78E-60	-1.69E-46
MS.gene056549	MS.gene68738	0.873593	1.30E-67	-1.69E-46
MS.gene056610	MS.gene68738	0.805351	1.39E-49	-1.69E-46
MS.gene056657	MS.gene68738	0.898569	4.66E-77	-1.69E-46
MS.gene056692	MS.gene68738	0.890755	7.40E-74	-1.69E-46
MS.gene056898	MS.gene68738	0.829229	5.79E-55	-1.69E-46
MS.gene057011	MS.gene68738	0.827721	1.34E-54	-1.69E-46
MS.gene057129	MS.gene68738	0.860732	1.68E-63	-1.69E-46
MS.gene057246	MS.gene68738	0.800268	1.56E-48	-1.69E-46
MS.gene057331	MS.gene68738	0.809942	1.47E-50	-1.69E-46
MS.gene057416	MS.gene68738	0.849628	2.86E-60	-1.69E-46
MS.gene057831	MS.gene68738	0.855064	8.10E-62	-1.69E-46
MS.gene058019	MS.gene68738	0.81696	4.22E-52	-1.69E-46
MS.gene058323	MS.gene68738	0.816078	6.66E-52	-1.69E-46
MS.gene058335	MS.gene68738	0.806892	6.60E-50	-1.69E-46
MS.gene058419	MS.gene68738	0.887856	9.88E-73	-1.69E-46
MS.gene058544	MS.gene68738	0.836204	1.07E-56	-1.69E-46
MS.gene058719	MS.gene68738	0.854864	9.26E-62	-1.69E-46
MS.gene058723	MS.gene68738	0.824595	7.41E-54	-1.69E-46
MS.gene058773	MS.gene68738	0.869787	2.38E-66	-1.69E-46
MS.gene05883	MS.gene68738	0.817534	3.14E-52	-1.69E-46
MS.gene059022	MS.gene68738	0.88233	1.14E-70	-1.69E-46
MS.gene059023	MS.gene68738	0.838128	3.46E-57	-1.69E-46
MS.gene059029	MS.gene68738	0.875193	3.74E-68	-1.69E-46
MS.gene059079	MS.gene68738	0.846079	2.72E-59	-1.69E-46
MS.gene05912	MS.gene68738	0.837352	5.47E-57	-1.69E-46
MS.gene05934	MS.gene68738	0.81957	1.08E-52	-1.69E-46
MS.gene059479	MS.gene68738	-0.822824	1.93E-53	-1.69E-46
MS.gene059536	MS.gene68738	0.810072	1.38E-50	-1.69E-46
MS.gene059677	MS.gene68738	0.902271	1.15E-78	-1.69E-46
MS.gene059718	MS.gene68738	0.866084	3.69E-65	-1.69E-46
MS.gene059756	MS.gene68738	0.864555	1.12E-64	-1.69E-46
MS.gene059794	MS.gene68738	0.806172	9.36E-50	-1.69E-46
MS.gene059801	MS.gene68738	0.824208	9.14E-54	-1.69E-46
MS.gene059858	MS.gene68738	0.814275	1.67E-51	-1.69E-46
MS.gene059865	MS.gene68738	0.828727	7.65E-55	-1.69E-46
MS.gene059902	MS.gene68738	0.814537	1.46E-51	-1.69E-46
MS.gene059960	MS.gene68738	0.875644	2.62E-68	-1.69E-46
MS.gene060193	MS.gene68738	0.807506	4.89E-50	-1.69E-46
MS.gene060703	MS.gene68738	0.853902	1.76E-61	-1.69E-46
MS.gene060762	MS.gene68738	0.866783	2.21E-65	-1.69E-46
MS.gene06077	MS.gene68738	0.871417	6.94E-67	-1.69E-46
MS.gene06092	MS.gene68738	0.826394	2.78E-54	-1.69E-46
MS.gene060982	MS.gene68738	0.81052	1.11E-50	-1.69E-46
MS.gene061134	MS.gene68738	0.835292	1.83E-56	-1.69E-46
MS.gene061150	MS.gene68738	0.903063	5.10E-79	-1.69E-46
MS.gene061171	MS.gene68738	0.887837	1.00E-72	-1.69E-46
MS.gene061200	MS.gene68738	0.875663	2.58E-68	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene68738	MS.gene004173	PPI
MS.gene23896	MS.gene68738	PPI
MS.gene002503	MS.gene68738	PPI
MS.gene027936	MS.gene68738	PPI
MS.gene97720	MS.gene68738	PPI
MS.gene01476	MS.gene68738	PPI
MS.gene68738	MS.gene02808	PPI
MS.gene68738	MS.gene027933	PPI
MS.gene97715	MS.gene68738	PPI
MS.gene23898	MS.gene68738	PPI
MS.gene051310	MS.gene68738	PPI
MS.gene027928	MS.gene68738	PPI
MS.gene68738	MS.gene051306	PPI
MS.gene051305	MS.gene68738	PPI
MS.gene027931	MS.gene68738	PPI
MS.gene047358	MS.gene68738	PPI
MS.gene78935	MS.gene68738	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68738.t1	MTR_3g118070	99.318	440	3	0	1	440	29	468	0.0	895
MS.gene68738.t1	MTR_3g118070	99.270	411	3	0	30	440	1	411	0.0	837
MS.gene68738.t1	MTR_7g451450	34.448	299	169	10	48	333	87	371	9.77e-34	132
MS.gene68738.t1	MTR_7g072420	27.946	297	197	8	48	332	83	374	4.25e-21	95.9
MS.gene68738.t1	MTR_3g465800	27.759	299	197	7	47	332	74	366	6.93e-19	89.0
MS.gene68738.t1	MTR_3g465800	28.223	287	187	7	59	332	15	295	8.98e-19	88.2
MS.gene68738.t1	MTR_8g091660	25.373	335	219	11	47	363	82	403	2.09e-17	84.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68738.t1	AT3G48730	83.182	440	72	1	1	440	35	472	0.0	767
MS.gene68738.t1	AT5G63570	80.909	440	82	1	1	440	37	474	0.0	747
MS.gene68738.t1	AT5G63570	82.725	411	71	0	30	440	1	411	0.0	717
MS.gene68738.t1	AT5G46180	28.882	322	204	8	48	356	59	368	3.22e-30	122
MS.gene68738.t1	AT1G80600	29.773	309	170	10	48	333	77	361	8.35e-26	109
MS.gene68738.t1	AT3G08860	26.667	315	206	11	48	346	83	388	2.05e-20	93.6
MS.gene68738.t1	AT3G08860	26.667	315	206	11	48	346	83	388	2.94e-20	93.2
MS.gene68738.t1	AT4G39660	25.519	337	216	11	47	363	77	398	8.56e-20	92.0
MS.gene68738.t1	AT4G39660	25.538	325	207	11	59	363	14	323	1.92e-19	90.1
MS.gene68738.t1	AT2G38400	25.444	338	214	10	47	363	79	399	2.36e-16	81.6
MS.gene68738.t1	AT2G38400	25.444	338	214	10	47	363	79	399	2.39e-16	81.6

Find 144 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAAGTTTGCTAAGTTCTTC+TGG	0.125787	3.2:+93116578	MS.gene68738:CDS
TCTTGGGGTCCTGCAATTAT+TGG	0.214742	3.2:+93115541	MS.gene68738:CDS
TTGACGGAATCAATGATAAT+TGG	0.243448	3.2:-93115466	None:intergenic
TGGAGGTAGGAGAGATATTA+TGG	0.268158	3.2:+93116279	MS.gene68738:CDS
CATTGACTATGTTGGTTCTT+GGG	0.275476	3.2:+93115525	MS.gene68738:CDS
GTTTCATTGGTGGCAGCTTT+AGG	0.281598	3.2:-93115956	None:intergenic
CTGCTGCTGAGAAGGTCTTT+AGG	0.282740	3.2:+93116698	MS.gene68738:CDS
TGCTGCTGAGAAGGTCTTTA+GGG	0.286640	3.2:+93116699	MS.gene68738:CDS
CTTGATGATCTTCTCTTTGC+CGG	0.295779	3.2:-93115840	None:intergenic
CGCAAGGAACTAAGAAAATC+AGG	0.299535	3.2:-93116100	None:intergenic
ATATGTGGTGGGCATATAAA+TGG	0.309935	3.2:+93116487	MS.gene68738:CDS
TTCCCCAGAGTTGTTATATC+AGG	0.310573	3.2:-93116223	None:intergenic
ACATTGACTATGTTGGTTCT+TGG	0.314847	3.2:+93115524	MS.gene68738:CDS
CAAGTTTGCTAAGTTCTTCT+GGG	0.335783	3.2:+93116579	MS.gene68738:CDS
AGAAACTCTGAAGCGTATTA+AGG	0.338134	3.2:+93116378	MS.gene68738:CDS
GAACCTGTTGTTGGAAATTC+CGG	0.340485	3.2:+93116061	MS.gene68738:CDS
GTTAGCAGAAGGTGTCTATT+TGG	0.340874	3.2:+93116606	MS.gene68738:CDS
GAATACTTGGACAAAATTAC+AGG	0.342820	3.2:+93116415	MS.gene68738:CDS
ATGGTCAGATTTGTCAATTC+CGG	0.345817	3.2:+93115773	MS.gene68738:CDS
GGCATATAAATGGGATGTTT+GGG	0.350134	3.2:+93116497	MS.gene68738:CDS
GGGCATATAAATGGGATGTT+TGG	0.352503	3.2:+93116496	MS.gene68738:CDS
GCACCTTCACAGTTTGAGGC+TGG	0.354758	3.2:+93116628	MS.gene68738:CDS
TCCAAGTATTCATAAGTTCC+TGG	0.359322	3.2:-93116403	None:intergenic
TTAGGAACACCAGGGGAATC+AGG	0.388149	3.2:-93115938	None:intergenic
TTTGGCACCTTCACAGTTTG+AGG	0.388856	3.2:+93116624	MS.gene68738:CDS
GGCTGTAAGGGTTTCATTGG+TGG	0.391375	3.2:-93115966	None:intergenic
GAACTAAGAAAATCAGGTTT+AGG	0.397600	3.2:-93116094	None:intergenic
TACGGCTATCTCTCCTTTGT+TGG	0.403214	3.2:-93116029	None:intergenic
TATTGGTCACGCTGATGATC+AGG	0.409676	3.2:+93115558	MS.gene68738:CDS
TGCTGTTCCCAGCATTGAAA+TGG	0.417543	3.2:+93115754	MS.gene68738:CDS
CCAGAAGAACTTAGCAAACT+TGG	0.420091	3.2:-93116578	None:intergenic
TTTCTTGTTAAGGCAGGTAG+TGG	0.428022	3.2:+93115896	MS.gene68738:CDS
ATGAAGAAAGGAACCAGCTT+TGG	0.429438	3.2:+93115686	MS.gene68738:CDS
GACGTTGAGTGGGAATCCTT+TGG	0.430505	3.2:+93116339	MS.gene68738:CDS
GTCTTCCTCGAACCTGTTGT+TGG	0.431603	3.2:+93116052	MS.gene68738:CDS
CACCTTCACAGTTTGAGGCT+GGG	0.433643	3.2:+93116629	MS.gene68738:CDS
CTACCTGCCTTAACAAGAAA+AGG	0.436782	3.2:-93115893	None:intergenic
GGTTTGCCAGTAGGTGCATA+TGG	0.445263	3.2:+93116259	MS.gene68738:CDS
AAACCGGAATTTCCAACAAC+AGG	0.445497	3.2:-93116064	None:intergenic
ACAACTCTGGGGAAGATCAT+TGG	0.450763	3.2:+93116232	MS.gene68738:CDS
CCACATGCGAGCACCTTTGA+CGG	0.452870	3.2:-93115482	None:intergenic
ACTGGATTCCGTTTGTCGTA+TGG	0.457113	3.2:+93116172	MS.gene68738:CDS
TCAGCGTGACCAATAATTGC+AGG	0.459493	3.2:-93115550	None:intergenic
ATTGACTATGTTGGTTCTTG+GGG	0.459541	3.2:+93115526	MS.gene68738:CDS
TGAGGCTGGGTTCACCAGCT+TGG	0.460390	3.2:+93116642	MS.gene68738:CDS
AAATCTGACCATTTCAATGC+TGG	0.463786	3.2:-93115762	None:intergenic
CGCCTTGCCCGTGCTTATAC+CGG	0.463976	3.2:+93115821	MS.gene68738:CDS
AACTCCTGATATAACAACTC+TGG	0.465327	3.2:+93116219	MS.gene68738:CDS
CAGGGGAATCAGGAAGTCCT+AGG	0.466845	3.2:-93115928	None:intergenic
TTTGATTAGGAATTGATGCC+TGG	0.467656	3.2:+93115397	MS.gene68738:intron
ACTCCTGATATAACAACTCT+GGG	0.468947	3.2:+93116220	MS.gene68738:CDS
ATTATCATTGATTCCGTCAA+AGG	0.468966	3.2:+93115469	MS.gene68738:CDS
ATGGAGACCGTGGCTCCGGC+TGG	0.468994	3.2:+93116298	MS.gene68738:CDS
AGAGAAGATCATCAAGTTTG+AGG	0.471292	3.2:+93115847	MS.gene68738:CDS
ATTACAGGTGAGCTTGTTCA+AGG	0.477356	3.2:+93116430	MS.gene68738:CDS
AAGACAAGGTGCACCAAAGC+TGG	0.477394	3.2:-93115699	None:intergenic
TTCCGGCACTGAAGCATGCA+TGG	0.479996	3.2:+93115790	MS.gene68738:CDS
GAGAAGATCATCAAGTTTGA+GGG	0.481387	3.2:+93115848	MS.gene68738:CDS
GACCATTGCTGCTGCTGAGA+AGG	0.485730	3.2:+93116690	MS.gene68738:CDS
CTCTTTGCCGGTATAAGCAC+GGG	0.486208	3.2:-93115828	None:intergenic
TTCGGTGCTGGGCTTCTGGT+CGG	0.486793	3.2:-93115233	None:intergenic
GTTCAAGGCATTGTTGAAGC+TGG	0.489925	3.2:+93116445	MS.gene68738:CDS
AACCAACATAGTCAATGTAT+TGG	0.491453	3.2:-93115519	None:intergenic
ACTGATTTGAAAGCACGAAC+AGG	0.495762	3.2:-93115433	None:intergenic
GTGTTTGATGAAGTTATGAC+TGG	0.497336	3.2:+93116154	MS.gene68738:CDS
GCCAGGAACTTATGAATACT+TGG	0.499909	3.2:+93116402	MS.gene68738:CDS
GCTGGACCTATGTACCAGGC+TGG	0.502027	3.2:+93116316	MS.gene68738:CDS
AACAGCAGCAAAGTCATTGA+AGG	0.503512	3.2:-93115990	None:intergenic
AATCCTTTGGCAATGACTGC+AGG	0.505080	3.2:+93116352	MS.gene68738:CDS
GTTCGTGCTTTCAAATCAGT+TGG	0.507920	3.2:+93115436	MS.gene68738:CDS
GAGCACCTCCATACGACAAA+CGG	0.508625	3.2:-93116180	None:intergenic
GTTGAAGCTGGTAAGAAGGC+AGG	0.509704	3.2:+93116457	MS.gene68738:CDS
AACCAATACATTGACTATGT+TGG	0.510475	3.2:+93115517	MS.gene68738:CDS
ACAGGAGAGTTCACACCTCC+AGG	0.510938	3.2:-93115415	None:intergenic
TATTATGGAGACCGTGGCTC+CGG	0.513114	3.2:+93116294	MS.gene68738:CDS
AACGTCCCAGCCTGGTACAT+AGG	0.518158	3.2:-93116322	None:intergenic
GCCTGGTACATAGGTCCAGC+CGG	0.518203	3.2:-93116313	None:intergenic
TTCTGGGGTATGTTAGCAGA+AGG	0.519712	3.2:+93116595	MS.gene68738:CDS
GACCTTCTCAGCAGCAGCAA+TGG	0.522852	3.2:-93116692	None:intergenic
CTAGGACTTCCTGATTCCCC+TGG	0.524552	3.2:+93115929	MS.gene68738:CDS
GGTGCTGGGCTTCTGGTCGG+TGG	0.527286	3.2:-93115230	None:intergenic
TGAAAACACTCTAGCTGAGA+TGG	0.530589	3.2:+93115724	MS.gene68738:CDS
ACCCATGCATGCTTCAGTGC+CGG	0.530882	3.2:-93115792	None:intergenic
CTACCTCCATATGCACCTAC+TGG	0.530999	3.2:-93116265	None:intergenic
CGTGCTTTCAAATCAGTTGG+TGG	0.531486	3.2:+93115439	MS.gene68738:CDS
CCGTCAAAGGTGCTCGCATG+TGG	0.532480	3.2:+93115482	MS.gene68738:CDS
TACCAGGCTGGGACGTTGAG+TGG	0.532692	3.2:+93116328	MS.gene68738:CDS
GAGAAGGGCATTGTTCTCCT+TGG	0.539822	3.2:-93116131	None:intergenic
CATTGTTGAAGCTGGTAAGA+AGG	0.540418	3.2:+93116453	MS.gene68738:CDS
AGGTTTAGGAGTAATGAAAC+CGG	0.540434	3.2:-93116080	None:intergenic
GGAGGTGCTCAACAATACTT+TGG	0.540803	3.2:+93116193	MS.gene68738:CDS
CTGAAGCGTATTAAGGAGCC+AGG	0.542721	3.2:+93116385	MS.gene68738:CDS
CTGGACCTATGTACCAGGCT+GGG	0.542962	3.2:+93116317	MS.gene68738:CDS
AATTTCCAACAACAGGTTCG+AGG	0.544211	3.2:-93116057	None:intergenic
TGCCGGTATAAGCACGGGCA+AGG	0.545023	3.2:-93115823	None:intergenic
ATCAGAAGTATGTGCCAAGC+TGG	0.549286	3.2:-93116656	None:intergenic
TCCGGCTGGACCTATGTACC+AGG	0.550164	3.2:+93116312	MS.gene68738:CDS
AATCTGACCATTTCAATGCT+GGG	0.551104	3.2:-93115761	None:intergenic
GCTCGCATGTGGGACATTGA+TGG	0.552562	3.2:+93115493	MS.gene68738:CDS
GGTAGTGGAGTTGCCACCCT+AGG	0.552592	3.2:+93115911	MS.gene68738:CDS
TCCCACTCAACGTCCCAGCC+TGG	0.554590	3.2:-93116330	None:intergenic
CTTGCTGAGACAATGAAGAA+AGG	0.555611	3.2:+93115674	MS.gene68738:CDS
TCTCTTTGCCGGTATAAGCA+CGG	0.568722	3.2:-93115829	None:intergenic
CATAGGTCCAGCCGGAGCCA+CGG	0.570397	3.2:-93116305	None:intergenic
GTGGCAGCTTTAGGAACACC+AGG	0.571077	3.2:-93115947	None:intergenic
ACAGCAGCAAAGTCATTGAA+GGG	0.578728	3.2:-93115989	None:intergenic
GCAGGGCATGCAATATGTGG+TGG	0.581592	3.2:+93116475	MS.gene68738:CDS
TATGTGGTGGGCATATAAAT+GGG	0.582602	3.2:+93116488	MS.gene68738:CDS
AAGTTTGCTAAGTTCTTCTG+GGG	0.584115	3.2:+93116580	MS.gene68738:CDS
TAACTTCATCAAACACGAGA+AGG	0.585499	3.2:-93116147	None:intergenic
TTGAAGCTGGTAAGAAGGCA+GGG	0.585619	3.2:+93116458	MS.gene68738:CDS
AGGGGAATCAGGAAGTCCTA+GGG	0.587377	3.2:-93115927	None:intergenic
GGATTCCGTTTGTCGTATGG+AGG	0.588634	3.2:+93116175	MS.gene68738:CDS
AAGTTTGAGGGATGTTATCA+CGG	0.591307	3.2:+93115860	MS.gene68738:CDS
AAGGCAGGGCATGCAATATG+TGG	0.593656	3.2:+93116472	MS.gene68738:CDS
CTTCATTGTCTCAGCAAGAG+CGG	0.594962	3.2:-93115669	None:intergenic
ATGCCTGCAGTCATTGCCAA+AGG	0.596171	3.2:-93116355	None:intergenic
ACTCTGGGGAAGATCATTGG+AGG	0.600178	3.2:+93116235	MS.gene68738:CDS
CAGTAGGTGCATATGGAGGT+AGG	0.601307	3.2:+93116266	MS.gene68738:CDS
AGAAGCTTTCGCAGCTGCAA+AGG	0.604865	3.2:+93115279	MS.gene68738:CDS
TCCGGCACTGAAGCATGCAT+GGG	0.605633	3.2:+93115791	MS.gene68738:CDS
CAGGGCATGCAATATGTGGT+GGG	0.605835	3.2:+93116476	MS.gene68738:CDS
ACCAGGCTGGGACGTTGAGT+GGG	0.609650	3.2:+93116329	MS.gene68738:CDS
CTGGGGAAGATCATTGGAGG+GGG	0.612965	3.2:+93116238	MS.gene68738:CDS
AACTTCATCAAACACGAGAA+GGG	0.614126	3.2:-93116146	None:intergenic
AACAGGTTCGAGGAAGACTA+CGG	0.621214	3.2:-93116047	None:intergenic
CGTCAAAGGTGCTCGCATGT+GGG	0.625959	3.2:+93115483	MS.gene68738:CDS
AACCCAGCCTCAAACTGTGA+AGG	0.634803	3.2:-93116631	None:intergenic
AAGATCTTTGAAGCCAACAA+AGG	0.638698	3.2:+93116016	MS.gene68738:CDS
GCATCTGTGAAATTGTACAC+AGG	0.641026	3.2:-93116538	None:intergenic
GGAATCAGGAAGTCCTAGGG+TGG	0.643441	3.2:-93115924	None:intergenic
CGGTATAAGCACGGGCAAGG+CGG	0.653099	3.2:-93115820	None:intergenic
CAGCAGCAAAGTCATTGAAG+GGG	0.654908	3.2:-93115988	None:intergenic
GAGAGATATTATGGAGACCG+TGG	0.661183	3.2:+93116288	MS.gene68738:CDS
TCTGGGGAAGATCATTGGAG+GGG	0.662523	3.2:+93116237	MS.gene68738:CDS
AGCAGCAAAGTCATTGAAGG+GGG	0.664856	3.2:-93115987	None:intergenic
CTCTGGGGAAGATCATTGGA+GGG	0.675119	3.2:+93116236	MS.gene68738:CDS
TAACAAGAAAAGGATCAGCA+TGG	0.678181	3.2:-93115883	None:intergenic
TTCCTTGCGCAAAATCACCA+AGG	0.686509	3.2:+93116114	MS.gene68738:CDS
TTGCCAGTAGGTGCATATGG+AGG	0.697062	3.2:+93116262	MS.gene68738:CDS
GGCAGCTTTAGGAACACCAG+GGG	0.703847	3.2:-93115945	None:intergenic
TGGCAGCTTTAGGAACACCA+GGG	0.708271	3.2:-93115946	None:intergenic
GATTAGGAATTGATGCCTGG+AGG	0.717601	3.2:+93115400	MS.gene68738:intron
CTCCTGATATAACAACTCTG+GGG	0.744293	3.2:+93116221	MS.gene68738:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTAAACATAAAAGTTTGA+AGG	-	chr3.2:93115638-93115657	None:intergenic	15.0%
!!!	AAACTTTTATGTTTAATTTC+AGG	+	chr3.2:93115640-93115659	MS.gene68738:intron	15.0%
!!!	ATGTAATAATCGATTTGATT+AGG	+	chr3.2:93115384-93115403	MS.gene68738:intron	20.0%
!	AATATATGAGAGAATTGAAG+AGG	-	chr3.2:93115603-93115622	None:intergenic	25.0%
	AACCAACATAGTCAATGTAT+TGG	-	chr3.2:93115522-93115541	None:intergenic	30.0%
	GAACTAAGAAAATCAGGTTT+AGG	-	chr3.2:93116097-93116116	None:intergenic	30.0%
	GAATACTTGGACAAAATTAC+AGG	+	chr3.2:93116415-93116434	MS.gene68738:CDS	30.0%
	TTGACGGAATCAATGATAAT+TGG	-	chr3.2:93115469-93115488	None:intergenic	30.0%
!	AACCAATACATTGACTATGT+TGG	+	chr3.2:93115517-93115536	MS.gene68738:CDS	30.0%
!!	ATTATCATTGATTCCGTCAA+AGG	+	chr3.2:93115469-93115488	MS.gene68738:CDS	30.0%
	AAATCTGACCATTTCAATGC+TGG	-	chr3.2:93115765-93115784	None:intergenic	35.0%
	AACTCCTGATATAACAACTC+TGG	+	chr3.2:93116219-93116238	MS.gene68738:CDS	35.0%
	AACTTCATCAAACACGAGAA+GGG	-	chr3.2:93116149-93116168	None:intergenic	35.0%
	AAGTTTGAGGGATGTTATCA+CGG	+	chr3.2:93115860-93115879	MS.gene68738:CDS	35.0%
	AATCTGACCATTTCAATGCT+GGG	-	chr3.2:93115764-93115783	None:intergenic	35.0%
	ACTCCTGATATAACAACTCT+GGG	+	chr3.2:93116220-93116239	MS.gene68738:CDS	35.0%
	AGAGAAGATCATCAAGTTTG+AGG	+	chr3.2:93115847-93115866	MS.gene68738:CDS	35.0%
	AGGTTTAGGAGTAATGAAAC+CGG	-	chr3.2:93116083-93116102	None:intergenic	35.0%
	ATGGTCAGATTTGTCAATTC+CGG	+	chr3.2:93115773-93115792	MS.gene68738:CDS	35.0%
	GAGAAGATCATCAAGTTTGA+GGG	+	chr3.2:93115848-93115867	MS.gene68738:CDS	35.0%
	GGCATATAAATGGGATGTTT+GGG	+	chr3.2:93116497-93116516	MS.gene68738:CDS	35.0%
	GTGTTTGATGAAGTTATGAC+TGG	+	chr3.2:93116154-93116173	MS.gene68738:CDS	35.0%
	TAACAAGAAAAGGATCAGCA+TGG	-	chr3.2:93115886-93115905	None:intergenic	35.0%
	TAACTTCATCAAACACGAGA+AGG	-	chr3.2:93116150-93116169	None:intergenic	35.0%
	TCCAAGTATTCATAAGTTCC+TGG	-	chr3.2:93116406-93116425	None:intergenic	35.0%
	TTTGATTAGGAATTGATGCC+TGG	+	chr3.2:93115397-93115416	MS.gene68738:intron	35.0%
!	TGCTGATCCTTTTCTTGTTA+AGG	+	chr3.2:93115886-93115905	MS.gene68738:CDS	35.0%
!	TTTGCGAAGAGTGAGTTTTT+CGG	-	chr3.2:93115254-93115273	None:intergenic	35.0%
!!	AAGATCTTTGAAGCCAACAA+AGG	+	chr3.2:93116016-93116035	MS.gene68738:CDS	35.0%
!!	AAGTTTGCTAAGTTCTTCTG+GGG	+	chr3.2:93116580-93116599	MS.gene68738:CDS	35.0%
!!	ACATTGACTATGTTGGTTCT+TGG	+	chr3.2:93115524-93115543	MS.gene68738:CDS	35.0%
!!	AGAAACTCTGAAGCGTATTA+AGG	+	chr3.2:93116378-93116397	MS.gene68738:CDS	35.0%
!!	AGAGTGTTTTCAAGAAGACA+AGG	-	chr3.2:93115716-93115735	None:intergenic	35.0%
!!	ATATGTGGTGGGCATATAAA+TGG	+	chr3.2:93116487-93116506	MS.gene68738:CDS	35.0%
!!	ATTGACTATGTTGGTTCTTG+GGG	+	chr3.2:93115526-93115545	MS.gene68738:CDS	35.0%
!!	CAAGTTTGCTAAGTTCTTCT+GGG	+	chr3.2:93116579-93116598	MS.gene68738:CDS	35.0%
!!	CATTGACTATGTTGGTTCTT+GGG	+	chr3.2:93115525-93115544	MS.gene68738:CDS	35.0%
!!	TATGTGGTGGGCATATAAAT+GGG	+	chr3.2:93116488-93116507	MS.gene68738:CDS	35.0%
!!	TTTGGGTTTTTCTTCACAGA+AGG	+	chr3.2:93116514-93116533	MS.gene68738:CDS	35.0%
	AAACCGGAATTTCCAACAAC+AGG	-	chr3.2:93116067-93116086	None:intergenic	40.0%
	AACAGCAGCAAAGTCATTGA+AGG	-	chr3.2:93115993-93116012	None:intergenic	40.0%
	AATTTCCAACAACAGGTTCG+AGG	-	chr3.2:93116060-93116079	None:intergenic	40.0%
	ACAGCAGCAAAGTCATTGAA+GGG	-	chr3.2:93115992-93116011	None:intergenic	40.0%
	ATGAAGAAAGGAACCAGCTT+TGG	+	chr3.2:93115686-93115705	MS.gene68738:CDS	40.0%
	CATTGTTGAAGCTGGTAAGA+AGG	+	chr3.2:93116453-93116472	MS.gene68738:CDS	40.0%
	CCAGAAGAACTTAGCAAACT+TGG	-	chr3.2:93116581-93116600	None:intergenic	40.0%
	CGCAAGGAACTAAGAAAATC+AGG	-	chr3.2:93116103-93116122	None:intergenic	40.0%
	CTACCTGCCTTAACAAGAAA+AGG	-	chr3.2:93115896-93115915	None:intergenic	40.0%
	CTCCTGATATAACAACTCTG+GGG	+	chr3.2:93116221-93116240	MS.gene68738:CDS	40.0%
	CTTGCTGAGACAATGAAGAA+AGG	+	chr3.2:93115674-93115693	MS.gene68738:CDS	40.0%
	GAACCTGTTGTTGGAAATTC+CGG	+	chr3.2:93116061-93116080	MS.gene68738:CDS	40.0%
	GCATCTGTGAAATTGTACAC+AGG	-	chr3.2:93116541-93116560	None:intergenic	40.0%
	GCCAGGAACTTATGAATACT+TGG	+	chr3.2:93116402-93116421	MS.gene68738:CDS	40.0%
	GGGCATATAAATGGGATGTT+TGG	+	chr3.2:93116496-93116515	MS.gene68738:CDS	40.0%
	GTTCGTGCTTTCAAATCAGT+TGG	+	chr3.2:93115436-93115455	MS.gene68738:CDS	40.0%
	TGAAAACACTCTAGCTGAGA+TGG	+	chr3.2:93115724-93115743	MS.gene68738:CDS	40.0%
	TGGAGGTAGGAGAGATATTA+TGG	+	chr3.2:93116279-93116298	MS.gene68738:CDS	40.0%
	TTCCCCAGAGTTGTTATATC+AGG	-	chr3.2:93116226-93116245	None:intergenic	40.0%
	TTTCTTGTTAAGGCAGGTAG+TGG	+	chr3.2:93115896-93115915	MS.gene68738:CDS	40.0%
!	ATTACAGGTGAGCTTGTTCA+AGG	+	chr3.2:93116430-93116449	MS.gene68738:CDS	40.0%
!	CTTGATGATCTTCTCTTTGC+CGG	-	chr3.2:93115843-93115862	None:intergenic	40.0%
!	GATCCTTTTCTTGTTAAGGC+AGG	+	chr3.2:93115890-93115909	MS.gene68738:CDS	40.0%
!!	ACTGATTTGAAAGCACGAAC+AGG	-	chr3.2:93115436-93115455	None:intergenic	40.0%
!!	CCAAGTTTGCTAAGTTCTTC+TGG	+	chr3.2:93116578-93116597	MS.gene68738:CDS	40.0%
!!	GTTAGCAGAAGGTGTCTATT+TGG	+	chr3.2:93116606-93116625	MS.gene68738:CDS	40.0%
	AACAGGTTCGAGGAAGACTA+CGG	-	chr3.2:93116050-93116069	None:intergenic	45.0%
	AATCCTTTGGCAATGACTGC+AGG	+	chr3.2:93116352-93116371	MS.gene68738:CDS	45.0%
	ACTGGATTCCGTTTGTCGTA+TGG	+	chr3.2:93116172-93116191	MS.gene68738:CDS	45.0%
	AGCAGCAAAGTCATTGAAGG+GGG	-	chr3.2:93115990-93116009	None:intergenic	45.0%
	CAGCAGCAAAGTCATTGAAG+GGG	-	chr3.2:93115991-93116010	None:intergenic	45.0%
	CGTGCTTTCAAATCAGTTGG+TGG	+	chr3.2:93115439-93115458	MS.gene68738:CDS	45.0%
	CTTCATTGTCTCAGCAAGAG+CGG	-	chr3.2:93115672-93115691	None:intergenic	45.0%
	GAGAGATATTATGGAGACCG+TGG	+	chr3.2:93116288-93116307	MS.gene68738:CDS	45.0%
	GATTAGGAATTGATGCCTGG+AGG	+	chr3.2:93115400-93115419	MS.gene68738:intron	45.0%
	TACGGCTATCTCTCCTTTGT+TGG	-	chr3.2:93116032-93116051	None:intergenic	45.0%
	TATTGGTCACGCTGATGATC+AGG	+	chr3.2:93115558-93115577	MS.gene68738:CDS	45.0%
	TCAGCGTGACCAATAATTGC+AGG	-	chr3.2:93115553-93115572	None:intergenic	45.0%
	TCTCTTTGCCGGTATAAGCA+CGG	-	chr3.2:93115832-93115851	None:intergenic	45.0%
	TCTTGGGGTCCTGCAATTAT+TGG	+	chr3.2:93115541-93115560	MS.gene68738:CDS	45.0%
	TGCTGCTGAGAAGGTCTTTA+GGG	+	chr3.2:93116699-93116718	MS.gene68738:CDS	45.0%
	TTCCTTGCGCAAAATCACCA+AGG	+	chr3.2:93116114-93116133	MS.gene68738:CDS	45.0%
	TTGAAGCTGGTAAGAAGGCA+GGG	+	chr3.2:93116458-93116477	MS.gene68738:CDS	45.0%
	TTTGGCACCTTCACAGTTTG+AGG	+	chr3.2:93116624-93116643	MS.gene68738:CDS	45.0%
!	ACAACTCTGGGGAAGATCAT+TGG	+	chr3.2:93116232-93116251	MS.gene68738:CDS	45.0%
!	ATCAGAAGTATGTGCCAAGC+TGG	-	chr3.2:93116659-93116678	None:intergenic	45.0%
!	GTTTCATTGGTGGCAGCTTT+AGG	-	chr3.2:93115959-93115978	None:intergenic	45.0%
!	TGCTGTTCCCAGCATTGAAA+TGG	+	chr3.2:93115754-93115773	MS.gene68738:CDS	45.0%
!!	AAGAGTGAGTTTTTCGGTGC+TGG	-	chr3.2:93115248-93115267	None:intergenic	45.0%
!!	GGAGGTGCTCAACAATACTT+TGG	+	chr3.2:93116193-93116212	MS.gene68738:CDS	45.0%
!!	GTTCAAGGCATTGTTGAAGC+TGG	+	chr3.2:93116445-93116464	MS.gene68738:CDS	45.0%
!!	TTCTGGGGTATGTTAGCAGA+AGG	+	chr3.2:93116595-93116614	MS.gene68738:CDS	45.0%
!!!	AGAGTGAGTTTTTCGGTGCT+GGG	-	chr3.2:93115247-93115266	None:intergenic	45.0%
	AACCCAGCCTCAAACTGTGA+AGG	-	chr3.2:93116634-93116653	None:intergenic	50.0%
	AAGACAAGGTGCACCAAAGC+TGG	-	chr3.2:93115702-93115721	None:intergenic	50.0%
	AAGGCAGGGCATGCAATATG+TGG	+	chr3.2:93116472-93116491	MS.gene68738:CDS	50.0%
	AGAAGCTTTCGCAGCTGCAA+AGG	+	chr3.2:93115279-93115298	MS.gene68738:CDS	50.0%
	AGGGGAATCAGGAAGTCCTA+GGG	-	chr3.2:93115930-93115949	None:intergenic	50.0%
	AGTCATTGAAGGGGGCTGTA+AGG	-	chr3.2:93115982-93116001	None:intergenic	50.0%
	ATGCCTGCAGTCATTGCCAA+AGG	-	chr3.2:93116358-93116377	None:intergenic	50.0%
	CAGGGCATGCAATATGTGGT+GGG	+	chr3.2:93116476-93116495	MS.gene68738:CDS	50.0%
	CAGTAGGTGCATATGGAGGT+AGG	+	chr3.2:93116266-93116285	MS.gene68738:CDS	50.0%
	CTACCTCCATATGCACCTAC+TGG	-	chr3.2:93116268-93116287	None:intergenic	50.0%
	CTCTTTGCCGGTATAAGCAC+GGG	-	chr3.2:93115831-93115850	None:intergenic	50.0%
	CTGAAGCGTATTAAGGAGCC+AGG	+	chr3.2:93116385-93116404	MS.gene68738:CDS	50.0%
	CTGCTGCTGAGAAGGTCTTT+AGG	+	chr3.2:93116698-93116717	MS.gene68738:CDS	50.0%
	GACGTTGAGTGGGAATCCTT+TGG	+	chr3.2:93116339-93116358	MS.gene68738:CDS	50.0%
	GAGCACCTCCATACGACAAA+CGG	-	chr3.2:93116183-93116202	None:intergenic	50.0%
	GGATTCCGTTTGTCGTATGG+AGG	+	chr3.2:93116175-93116194	MS.gene68738:CDS	50.0%
	GGCTGTAAGGGTTTCATTGG+TGG	-	chr3.2:93115969-93115988	None:intergenic	50.0%
	GGTTTGCCAGTAGGTGCATA+TGG	+	chr3.2:93116259-93116278	MS.gene68738:CDS	50.0%
	GTCATTGAAGGGGGCTGTAA+GGG	-	chr3.2:93115981-93116000	None:intergenic	50.0%
	GTCTTCCTCGAACCTGTTGT+TGG	+	chr3.2:93116052-93116071	MS.gene68738:CDS	50.0%
	GTTGAAGCTGGTAAGAAGGC+AGG	+	chr3.2:93116457-93116476	MS.gene68738:CDS	50.0%
	TATTATGGAGACCGTGGCTC+CGG	+	chr3.2:93116294-93116313	MS.gene68738:CDS	50.0%
	TCTGGGGAAGATCATTGGAG+GGG	+	chr3.2:93116237-93116256	MS.gene68738:CDS	50.0%
	TTAGGAACACCAGGGGAATC+AGG	-	chr3.2:93115941-93115960	None:intergenic	50.0%
	TTGCCAGTAGGTGCATATGG+AGG	+	chr3.2:93116262-93116281	MS.gene68738:CDS	50.0%
!	ACTCTGGGGAAGATCATTGG+AGG	+	chr3.2:93116235-93116254	MS.gene68738:CDS	50.0%
!	CACCTTCACAGTTTGAGGCT+GGG	+	chr3.2:93116629-93116648	MS.gene68738:CDS	50.0%
!	CTCTGGGGAAGATCATTGGA+GGG	+	chr3.2:93116236-93116255	MS.gene68738:CDS	50.0%
!!	CTCCTTGGTGATTTTGCGCA+AGG	-	chr3.2:93116119-93116138	None:intergenic	50.0%
!!	GAGAAGGGCATTGTTCTCCT+TGG	-	chr3.2:93116134-93116153	None:intergenic	50.0%
!!	TGGCAGCTTTAGGAACACCA+GGG	-	chr3.2:93115949-93115968	None:intergenic	50.0%
	AACGTCCCAGCCTGGTACAT+AGG	-	chr3.2:93116325-93116344	None:intergenic	55.0%
	ACAGGAGAGTTCACACCTCC+AGG	-	chr3.2:93115418-93115437	None:intergenic	55.0%
	ACCCATGCATGCTTCAGTGC+CGG	-	chr3.2:93115795-93115814	None:intergenic	55.0%
	ATTGGAGGGGGTTTGCCAGT+AGG	+	chr3.2:93116250-93116269	MS.gene68738:CDS	55.0%
	CAGGGGAATCAGGAAGTCCT+AGG	-	chr3.2:93115931-93115950	None:intergenic	55.0%
	CCACATGCGAGCACCTTTGA+CGG	-	chr3.2:93115485-93115504	None:intergenic	55.0%
	CTAGGACTTCCTGATTCCCC+TGG	+	chr3.2:93115929-93115948	MS.gene68738:CDS	55.0%
	CTGGACCTATGTACCAGGCT+GGG	+	chr3.2:93116317-93116336	MS.gene68738:CDS	55.0%
	CTGGGGAAGATCATTGGAGG+GGG	+	chr3.2:93116238-93116257	MS.gene68738:CDS	55.0%
	GACCATTGCTGCTGCTGAGA+AGG	+	chr3.2:93116690-93116709	MS.gene68738:CDS	55.0%
	GACCTTCTCAGCAGCAGCAA+TGG	-	chr3.2:93116695-93116714	None:intergenic	55.0%
	GCAGGGCATGCAATATGTGG+TGG	+	chr3.2:93116475-93116494	MS.gene68738:CDS	55.0%
	GCTCGCATGTGGGACATTGA+TGG	+	chr3.2:93115493-93115512	MS.gene68738:CDS	55.0%
	GGAATCAGGAAGTCCTAGGG+TGG	-	chr3.2:93115927-93115946	None:intergenic	55.0%
	GGGGGCTGTAAGGGTTTCAT+TGG	-	chr3.2:93115972-93115991	None:intergenic	55.0%
!	GCACCTTCACAGTTTGAGGC+TGG	+	chr3.2:93116628-93116647	MS.gene68738:CDS	55.0%
!	TCCGGCACTGAAGCATGCAT+GGG	+	chr3.2:93115791-93115810	MS.gene68738:CDS	55.0%
!	TTCCGGCACTGAAGCATGCA+TGG	+	chr3.2:93115790-93115809	MS.gene68738:CDS	55.0%
!!	CGTCAAAGGTGCTCGCATGT+GGG	+	chr3.2:93115483-93115502	MS.gene68738:CDS	55.0%
!!	GGCAGCTTTAGGAACACCAG+GGG	-	chr3.2:93115948-93115967	None:intergenic	55.0%
!!	GTGGCAGCTTTAGGAACACC+AGG	-	chr3.2:93115950-93115969	None:intergenic	55.0%
!!!	GTTTTTCGGTGCTGGGCTTC+TGG	-	chr3.2:93115240-93115259	None:intergenic	55.0%
	ACCAGGCTGGGACGTTGAGT+GGG	+	chr3.2:93116329-93116348	MS.gene68738:CDS	60.0%
	CGCCTTGCCCGTGCTTATAC+CGG	+	chr3.2:93115821-93115840	MS.gene68738:CDS	60.0%
	CGGTATAAGCACGGGCAAGG+CGG	-	chr3.2:93115823-93115842	None:intergenic	60.0%
	GCCTGGTACATAGGTCCAGC+CGG	-	chr3.2:93116316-93116335	None:intergenic	60.0%
	GCTGGACCTATGTACCAGGC+TGG	+	chr3.2:93116316-93116335	MS.gene68738:CDS	60.0%
	TACCAGGCTGGGACGTTGAG+TGG	+	chr3.2:93116328-93116347	MS.gene68738:CDS	60.0%
	TCCGGCTGGACCTATGTACC+AGG	+	chr3.2:93116312-93116331	MS.gene68738:CDS	60.0%
	TGAGGCTGGGTTCACCAGCT+TGG	+	chr3.2:93116642-93116661	MS.gene68738:CDS	60.0%
	TGCCGGTATAAGCACGGGCA+AGG	-	chr3.2:93115826-93115845	None:intergenic	60.0%
!!	CCGTCAAAGGTGCTCGCATG+TGG	+	chr3.2:93115482-93115501	MS.gene68738:CDS	60.0%
!!	GGTAGTGGAGTTGCCACCCT+AGG	+	chr3.2:93115911-93115930	MS.gene68738:CDS	60.0%
!!	TTCGGTGCTGGGCTTCTGGT+CGG	-	chr3.2:93115236-93115255	None:intergenic	60.0%
	CATAGGTCCAGCCGGAGCCA+CGG	-	chr3.2:93116308-93116327	None:intergenic	65.0%
	TCCCACTCAACGTCCCAGCC+TGG	-	chr3.2:93116333-93116352	None:intergenic	65.0%
	ATGGAGACCGTGGCTCCGGC+TGG	+	chr3.2:93116298-93116317	MS.gene68738:CDS	70.0%
!	GGTGCTGGGCTTCTGGTCGG+TGG	-	chr3.2:93115233-93115252	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	93115220	93116729	93115220	ID=MS.gene68738
chr3.2	mRNA	93115220	93116729	93115220	ID=MS.gene68738.t1;Parent=MS.gene68738
chr3.2	exon	93115220	93115300	93115220	ID=MS.gene68738.t1.exon1;Parent=MS.gene68738.t1
chr3.2	CDS	93115220	93115300	93115220	ID=cds.MS.gene68738.t1;Parent=MS.gene68738.t1
chr3.2	exon	93115406	93115579	93115406	ID=MS.gene68738.t1.exon2;Parent=MS.gene68738.t1
chr3.2	CDS	93115406	93115579	93115406	ID=cds.MS.gene68738.t1;Parent=MS.gene68738.t1
chr3.2	exon	93115662	93116729	93115662	ID=MS.gene68738.t1.exon3;Parent=MS.gene68738.t1
chr3.2	CDS	93115662	93116729	93115662	ID=cds.MS.gene68738.t1;Parent=MS.gene68738.t1

Gene Sequence

>MS.gene68738

ATGGCTGTTTCCACCGACCAGAAGCCCAGCACCGAAAAACTCACTCTTCGCAAATCTGAAGAAGCTTTCGCAGCTGCAAAGGTAATTCTTCTTCTTCTTCTTCGCTTTTTCATCAACATCATCATCATCATCATCATCATCATCATCTAATAATAATAATAATAATGTAATAATCGATTTGATTAGGAATTGATGCCTGGAGGTGTGAACTCTCCTGTTCGTGCTTTCAAATCAGTTGGTGGACAACCAATTATCATTGATTCCGTCAAAGGTGCTCGCATGTGGGACATTGATGGCAACCAATACATTGACTATGTTGGTTCTTGGGGTCCTGCAATTATTGGTCACGCTGATGATCAGGTTTCTTATTTTTAACATAACCTCTTCAATTCTCTCATATATTGCTGCTGTTTATCCTTCAAACTTTTATGTTTAATTTCAGGTACTCTCCGCTCTTGCTGAGACAATGAAGAAAGGAACCAGCTTTGGTGCACCTTGTCTTCTTGAAAACACTCTAGCTGAGATGGTTATCTCTGCTGTTCCCAGCATTGAAATGGTCAGATTTGTCAATTCCGGCACTGAAGCATGCATGGGTGCACTCCGCCTTGCCCGTGCTTATACCGGCAAAGAGAAGATCATCAAGTTTGAGGGATGTTATCACGGCCATGCTGATCCTTTTCTTGTTAAGGCAGGTAGTGGAGTTGCCACCCTAGGACTTCCTGATTCCCCTGGTGTTCCTAAAGCTGCCACCAATGAAACCCTTACAGCCCCCTTCAATGACTTTGCTGCTGTTGAGAAGATCTTTGAAGCCAACAAAGGAGAGATAGCCGTAGTCTTCCTCGAACCTGTTGTTGGAAATTCCGGTTTCATTACTCCTAAACCTGATTTTCTTAGTTCCTTGCGCAAAATCACCAAGGAGAACAATGCCCTTCTCGTGTTTGATGAAGTTATGACTGGATTCCGTTTGTCGTATGGAGGTGCTCAACAATACTTTGGCATAACTCCTGATATAACAACTCTGGGGAAGATCATTGGAGGGGGTTTGCCAGTAGGTGCATATGGAGGTAGGAGAGATATTATGGAGACCGTGGCTCCGGCTGGACCTATGTACCAGGCTGGGACGTTGAGTGGGAATCCTTTGGCAATGACTGCAGGCATAGAAACTCTGAAGCGTATTAAGGAGCCAGGAACTTATGAATACTTGGACAAAATTACAGGTGAGCTTGTTCAAGGCATTGTTGAAGCTGGTAAGAAGGCAGGGCATGCAATATGTGGTGGGCATATAAATGGGATGTTTGGGTTTTTCTTCACAGAAGGACCTGTGTACAATTTCACAGATGCAAAAAAGAGTGACACTGCCAAGTTTGCTAAGTTCTTCTGGGGTATGTTAGCAGAAGGTGTCTATTTGGCACCTTCACAGTTTGAGGCTGGGTTCACCAGCTTGGCACATACTTCTGATGACATCAAGAAGACCATTGCTGCTGCTGAGAAGGTCTTTAGGGAGATCTGA

Protein sequence

>MS.gene68738.t1

MAVSTDQKPSTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGARMWDIDGNQYIDYVGSWGPAIIGHADDQVLSALAETMKKGTSFGAPCLLENTLAEMVISAVPSIEMVRFVNSGTEACMGALRLARAYTGKEKIIKFEGCYHGHADPFLVKAGSGVATLGLPDSPGVPKAATNETLTAPFNDFAAVEKIFEANKGEIAVVFLEPVVGNSGFITPKPDFLSSLRKITKENNALLVFDEVMTGFRLSYGGAQQYFGITPDITTLGKIIGGGLPVGAYGGRRDIMETVAPAGPMYQAGTLSGNPLAMTAGIETLKRIKEPGTYEYLDKITGELVQGIVEAGKKAGHAICGGHINGMFGFFFTEGPVYNFTDAKKSDTAKFAKFFWGMLAEGVYLAPSQFEAGFTSLAHTSDDIKKTIAAAEKVFREI