Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85478.t1 | XP_003598690.1 | 97.8 | 179 | 4 | 0 | 1 | 179 | 1 | 179 | 1.40E-89 | 339 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85478.t1 | Q8VY88 | 71.9 | 167 | 42 | 2 | 14 | 179 | 13 | 175 | 4.5e-57 | 222.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85478.t1 | G7IW52 | 97.8 | 179 | 4 | 0 | 1 | 179 | 1 | 179 | 9.9e-90 | 339.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene85478 | 0.822713 | 2.05E-53 | -1.69E-46 |
| MS.gene049173 | MS.gene85478 | 0.819913 | 9.05E-53 | -1.69E-46 |
| MS.gene049217 | MS.gene85478 | 0.850616 | 1.51E-60 | -1.69E-46 |
| MS.gene049222 | MS.gene85478 | 0.825169 | 5.43E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene85478 | 0.821039 | 4.99E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene85478 | 0.800856 | 1.18E-48 | -1.69E-46 |
| MS.gene049351 | MS.gene85478 | 0.827838 | 1.25E-54 | -1.69E-46 |
| MS.gene049425 | MS.gene85478 | -0.811784 | 5.89E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene85478 | 0.83895 | 2.12E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene85478 | 0.817752 | 2.80E-52 | -1.69E-46 |
| MS.gene049614 | MS.gene85478 | 0.809166 | 2.16E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene85478 | 0.840897 | 6.59E-58 | -1.69E-46 |
| MS.gene049662 | MS.gene85478 | 0.831191 | 1.92E-55 | -1.69E-46 |
| MS.gene049760 | MS.gene85478 | 0.802083 | 6.64E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene85478 | 0.846711 | 1.83E-59 | -1.69E-46 |
| MS.gene050076 | MS.gene85478 | 0.861408 | 1.05E-63 | -1.69E-46 |
| MS.gene050083 | MS.gene85478 | -0.805185 | 1.51E-49 | -1.69E-46 |
| MS.gene050163 | MS.gene85478 | 0.824021 | 1.01E-53 | -1.69E-46 |
| MS.gene050165 | MS.gene85478 | 0.809166 | 2.16E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene85478 | 0.815981 | 7.00E-52 | -1.69E-46 |
| MS.gene050333 | MS.gene85478 | 0.815117 | 1.09E-51 | -1.69E-46 |
| MS.gene050397 | MS.gene85478 | 0.819553 | 1.09E-52 | -1.69E-46 |
| MS.gene050432 | MS.gene85478 | 0.847276 | 1.28E-59 | -1.69E-46 |
| MS.gene050444 | MS.gene85478 | 0.828274 | 9.85E-55 | -1.69E-46 |
| MS.gene050475 | MS.gene85478 | -0.824913 | 6.24E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene85478 | 0.841376 | 4.93E-58 | -1.69E-46 |
| MS.gene050541 | MS.gene85478 | -0.823491 | 1.35E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene85478 | 0.805206 | 1.49E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene85478 | 0.809623 | 1.73E-50 | -1.69E-46 |
| MS.gene050767 | MS.gene85478 | 0.867117 | 1.73E-65 | -1.69E-46 |
| MS.gene050770 | MS.gene85478 | 0.869873 | 2.23E-66 | -1.69E-46 |
| MS.gene050772 | MS.gene85478 | 0.830913 | 2.25E-55 | -1.69E-46 |
| MS.gene05079 | MS.gene85478 | -0.800895 | 1.16E-48 | -1.69E-46 |
| MS.gene05100 | MS.gene85478 | 0.821407 | 4.10E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene85478 | 0.840677 | 7.53E-58 | -1.69E-46 |
| MS.gene05105 | MS.gene85478 | 0.838833 | 2.27E-57 | -1.69E-46 |
| MS.gene051228 | MS.gene85478 | 0.877496 | 6.00E-69 | -1.69E-46 |
| MS.gene051471 | MS.gene85478 | 0.821644 | 3.62E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene85478 | 0.879638 | 1.06E-69 | -1.69E-46 |
| MS.gene051692 | MS.gene85478 | 0.812034 | 5.19E-51 | -1.69E-46 |
| MS.gene051694 | MS.gene85478 | 0.824985 | 6.00E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene85478 | 0.814392 | 1.58E-51 | -1.69E-46 |
| MS.gene051858 | MS.gene85478 | 0.854286 | 1.36E-61 | -1.69E-46 |
| MS.gene051877 | MS.gene85478 | 0.82653 | 2.58E-54 | -1.69E-46 |
| MS.gene051949 | MS.gene85478 | 0.830173 | 3.41E-55 | -1.69E-46 |
| MS.gene052078 | MS.gene85478 | 0.871141 | 8.57E-67 | -1.69E-46 |
| MS.gene052188 | MS.gene85478 | 0.821609 | 3.69E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene85478 | 0.824174 | 9.31E-54 | -1.69E-46 |
| MS.gene052290 | MS.gene85478 | 0.878811 | 2.08E-69 | -1.69E-46 |
| MS.gene052310 | MS.gene85478 | 0.864759 | 9.65E-65 | -1.69E-46 |
| MS.gene052322 | MS.gene85478 | 0.815123 | 1.09E-51 | -1.69E-46 |
| MS.gene052326 | MS.gene85478 | 0.834006 | 3.85E-56 | -1.69E-46 |
| MS.gene052379 | MS.gene85478 | 0.874302 | 7.51E-68 | -1.69E-46 |
| MS.gene052421 | MS.gene85478 | 0.81805 | 2.40E-52 | -1.69E-46 |
| MS.gene052484 | MS.gene85478 | 0.816518 | 5.31E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene85478 | 0.803239 | 3.83E-49 | -1.69E-46 |
| MS.gene052870 | MS.gene85478 | 0.828429 | 9.03E-55 | -1.69E-46 |
| MS.gene052872 | MS.gene85478 | 0.821229 | 4.51E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene85478 | 0.863684 | 2.09E-64 | -1.69E-46 |
| MS.gene053103 | MS.gene85478 | 0.870779 | 1.13E-66 | -1.69E-46 |
| MS.gene053104 | MS.gene85478 | 0.883662 | 3.71E-71 | -1.69E-46 |
| MS.gene053207 | MS.gene85478 | 0.830282 | 3.21E-55 | -1.69E-46 |
| MS.gene053209 | MS.gene85478 | 0.839456 | 1.57E-57 | -1.69E-46 |
| MS.gene053213 | MS.gene85478 | 0.806948 | 6.42E-50 | -1.69E-46 |
| MS.gene053322 | MS.gene85478 | 0.882058 | 1.43E-70 | -1.69E-46 |
| MS.gene053384 | MS.gene85478 | 0.825906 | 3.63E-54 | -1.69E-46 |
| MS.gene053632 | MS.gene85478 | 0.858619 | 7.28E-63 | -1.69E-46 |
| MS.gene053915 | MS.gene85478 | 0.819407 | 1.18E-52 | -1.69E-46 |
| MS.gene053916 | MS.gene85478 | 0.843921 | 1.04E-58 | -1.69E-46 |
| MS.gene054123 | MS.gene85478 | 0.814151 | 1.78E-51 | -1.69E-46 |
| MS.gene054290 | MS.gene85478 | -0.813411 | 2.59E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene85478 | 0.862999 | 3.40E-64 | -1.69E-46 |
| MS.gene054600 | MS.gene85478 | 0.802183 | 6.33E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene85478 | 0.847419 | 1.17E-59 | -1.69E-46 |
| MS.gene054802 | MS.gene85478 | 0.860605 | 1.84E-63 | -1.69E-46 |
| MS.gene054803 | MS.gene85478 | 0.861529 | 9.63E-64 | -1.69E-46 |
| MS.gene054804 | MS.gene85478 | 0.863984 | 1.68E-64 | -1.69E-46 |
| MS.gene054955 | MS.gene85478 | 0.857283 | 1.81E-62 | -1.69E-46 |
| MS.gene05505 | MS.gene85478 | 0.848196 | 7.15E-60 | -1.69E-46 |
| MS.gene055116 | MS.gene85478 | 0.813931 | 1.99E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene85478 | 0.826155 | 3.17E-54 | -1.69E-46 |
| MS.gene055212 | MS.gene85478 | 0.803238 | 3.84E-49 | -1.69E-46 |
| MS.gene055254 | MS.gene85478 | 0.877849 | 4.52E-69 | -1.69E-46 |
| MS.gene055277 | MS.gene85478 | 0.849233 | 3.69E-60 | -1.69E-46 |
| MS.gene055289 | MS.gene85478 | 0.80761 | 4.65E-50 | -1.69E-46 |
| MS.gene055397 | MS.gene85478 | 0.869468 | 3.03E-66 | -1.69E-46 |
| MS.gene055422 | MS.gene85478 | 0.8151 | 1.10E-51 | -1.69E-46 |
| MS.gene055499 | MS.gene85478 | 0.861694 | 8.57E-64 | -1.69E-46 |
| MS.gene055502 | MS.gene85478 | 0.811798 | 5.85E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene85478 | 0.814476 | 1.51E-51 | -1.69E-46 |
| MS.gene055636 | MS.gene85478 | 0.811048 | 8.51E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene85478 | 0.814785 | 1.29E-51 | -1.69E-46 |
| MS.gene055722 | MS.gene85478 | 0.832298 | 1.02E-55 | -1.69E-46 |
| MS.gene055896 | MS.gene85478 | 0.837293 | 5.66E-57 | -1.69E-46 |
| MS.gene05592 | MS.gene85478 | 0.857338 | 1.75E-62 | -1.69E-46 |
| MS.gene055941 | MS.gene85478 | 0.828205 | 1.02E-54 | -1.69E-46 |
| MS.gene05602 | MS.gene85478 | 0.811828 | 5.76E-51 | -1.69E-46 |
| MS.gene056083 | MS.gene85478 | 0.832817 | 7.61E-56 | -1.69E-46 |
| MS.gene056374 | MS.gene85478 | 0.801029 | 1.09E-48 | -1.69E-46 |
| MS.gene056407 | MS.gene85478 | 0.801168 | 1.02E-48 | -1.69E-46 |
| MS.gene056410 | MS.gene85478 | 0.822125 | 2.80E-53 | -1.69E-46 |
| MS.gene056487 | MS.gene85478 | 0.803625 | 3.19E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene85478 | 0.808037 | 3.77E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene85478 | 0.880845 | 3.92E-70 | -1.69E-46 |
| MS.gene056612 | MS.gene85478 | 0.807587 | 4.70E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene85478 | 0.811588 | 6.49E-51 | -1.69E-46 |
| MS.gene056719 | MS.gene85478 | 0.805819 | 1.11E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene85478 | 0.832385 | 9.74E-56 | -1.69E-46 |
| MS.gene056897 | MS.gene85478 | 0.821671 | 3.57E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene85478 | 0.830545 | 2.77E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene85478 | 0.866438 | 2.85E-65 | -1.69E-46 |
| MS.gene057129 | MS.gene85478 | 0.894374 | 2.62E-75 | -1.69E-46 |
| MS.gene057146 | MS.gene85478 | 0.858664 | 7.05E-63 | -1.69E-46 |
| MS.gene057246 | MS.gene85478 | 0.820028 | 8.52E-53 | -1.69E-46 |
| MS.gene057295 | MS.gene85478 | 0.856744 | 2.62E-62 | -1.69E-46 |
| MS.gene057416 | MS.gene85478 | 0.844972 | 5.43E-59 | -1.69E-46 |
| MS.gene057926 | MS.gene85478 | 0.852471 | 4.52E-61 | -1.69E-46 |
| MS.gene058006 | MS.gene85478 | 0.805106 | 1.57E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene85478 | 0.838192 | 3.33E-57 | -1.69E-46 |
| MS.gene058082 | MS.gene85478 | 0.864207 | 1.44E-64 | -1.69E-46 |
| MS.gene058233 | MS.gene85478 | 0.821824 | 3.29E-53 | -1.69E-46 |
| MS.gene058293 | MS.gene85478 | 0.872484 | 3.07E-67 | -1.69E-46 |
| MS.gene058323 | MS.gene85478 | 0.809801 | 1.58E-50 | -1.69E-46 |
| MS.gene058365 | MS.gene85478 | 0.81708 | 3.97E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene85478 | 0.822909 | 1.84E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene85478 | 0.887946 | 9.12E-73 | -1.69E-46 |
| MS.gene058723 | MS.gene85478 | 0.847885 | 8.71E-60 | -1.69E-46 |
| MS.gene058748 | MS.gene85478 | 0.822414 | 2.40E-53 | -1.69E-46 |
| MS.gene058787 | MS.gene85478 | 0.80626 | 8.97E-50 | -1.69E-46 |
| MS.gene058840 | MS.gene85478 | -0.801693 | 7.98E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene85478 | 0.810293 | 1.24E-50 | -1.69E-46 |
| MS.gene058993 | MS.gene85478 | 0.826171 | 3.14E-54 | -1.69E-46 |
| MS.gene059029 | MS.gene85478 | 0.837273 | 5.73E-57 | -1.69E-46 |
| MS.gene05913 | MS.gene85478 | 0.819614 | 1.06E-52 | -1.69E-46 |
| MS.gene059326 | MS.gene85478 | 0.824957 | 6.09E-54 | -1.69E-46 |
| MS.gene05934 | MS.gene85478 | 0.852317 | 5.00E-61 | -1.69E-46 |
| MS.gene059536 | MS.gene85478 | 0.875302 | 3.43E-68 | -1.69E-46 |
| MS.gene059597 | MS.gene85478 | 0.855329 | 6.79E-62 | -1.69E-46 |
| MS.gene05959 | MS.gene85478 | 0.827041 | 1.95E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene85478 | 0.860115 | 2.59E-63 | -1.69E-46 |
| MS.gene059677 | MS.gene85478 | 0.817816 | 2.71E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene85478 | 0.852701 | 3.88E-61 | -1.69E-46 |
| MS.gene059692 | MS.gene85478 | 0.865028 | 7.95E-65 | -1.69E-46 |
| MS.gene059695 | MS.gene85478 | 0.866229 | 3.32E-65 | -1.69E-46 |
| MS.gene059718 | MS.gene85478 | 0.836735 | 7.86E-57 | -1.69E-46 |
| MS.gene059858 | MS.gene85478 | 0.800894 | 1.16E-48 | -1.69E-46 |
| MS.gene059894 | MS.gene85478 | 0.804817 | 1.80E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene85478 | 0.858663 | 7.06E-63 | -1.69E-46 |
| MS.gene059960 | MS.gene85478 | 0.893053 | 8.99E-75 | -1.69E-46 |
| MS.gene060193 | MS.gene85478 | 0.805626 | 1.22E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene85478 | 0.804851 | 1.77E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene85478 | 0.888812 | 4.24E-73 | -1.69E-46 |
| MS.gene060818 | MS.gene85478 | 0.871434 | 6.85E-67 | -1.69E-46 |
| MS.gene060835 | MS.gene85478 | 0.803667 | 3.12E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene85478 | 0.808426 | 3.11E-50 | -1.69E-46 |
| MS.gene06090 | MS.gene85478 | 0.805995 | 1.02E-49 | -1.69E-46 |
| MS.gene06092 | MS.gene85478 | 0.811984 | 5.33E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene85478 | 0.884559 | 1.73E-71 | -1.69E-46 |
| MS.gene061049 | MS.gene85478 | 0.811993 | 5.30E-51 | -1.69E-46 |
| MS.gene061051 | MS.gene85478 | -0.826661 | 2.40E-54 | -1.69E-46 |
| MS.gene061150 | MS.gene85478 | 0.827915 | 1.20E-54 | -1.69E-46 |
| MS.gene061200 | MS.gene85478 | 0.878923 | 1.90E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85478.t1 | MTR_3g019320 | 97.765 | 179 | 4 | 0 | 1 | 179 | 1 | 179 | 1.19e-129 | 361 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85478.t1 | AT1G50900 | 82.308 | 130 | 22 | 1 | 51 | 179 | 46 | 175 | 2.15e-77 | 229 |
Find 43 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAGAGTTCCTCAATTT+TGG | 0.096587 | 3.4:-12676354 | None:intergenic |
| TTTGCAGTACTTCAACTATA+TGG | 0.192150 | 3.4:+12676223 | MS.gene85478:intron |
| GCTGAAGGTGCTGGTATTTA+TGG | 0.229748 | 3.4:+12674923 | MS.gene85478:CDS |
| AGAAACTTGTGAAGTGATTA+AGG | 0.234288 | 3.4:-12674756 | None:intergenic |
| TCAAGCTTCACCTCTAAATT+TGG | 0.237887 | 3.4:+12674842 | MS.gene85478:CDS |
| TCTAAATTTGGACCCTCTAA+TGG | 0.274812 | 3.4:+12674854 | MS.gene85478:CDS |
| CCACAAACTTGTCTTCTTCT+CGG | 0.286283 | 3.4:+12674780 | MS.gene85478:CDS |
| TTGTCTTCTTCTCGGTTTCT+TGG | 0.293903 | 3.4:+12674788 | MS.gene85478:CDS |
| TGTCCACGGGGTGAATGTTT+TGG | 0.329010 | 3.4:-12676295 | None:intergenic |
| TGAAGGAACATATGATAAAA+TGG | 0.336787 | 3.4:+12676259 | MS.gene85478:CDS |
| TTCACATGCTGGTTCAAGTT+TGG | 0.342748 | 3.4:+12674884 | MS.gene85478:CDS |
| TTGCAGTACTTCAACTATAT+GGG | 0.355490 | 3.4:+12676224 | MS.gene85478:intron |
| GGATTTAGAATTTGAAATGA+TGG | 0.407382 | 3.4:-12674735 | None:intergenic |
| TGATCGAGATGATGTTGAAC+AGG | 0.423533 | 3.4:+12674967 | MS.gene85478:CDS |
| CTAAATTTGGACCCTCTAAT+GGG | 0.424402 | 3.4:+12674855 | MS.gene85478:CDS |
| TGTGAATCTTGACCCATTAG+AGG | 0.437901 | 3.4:-12674867 | None:intergenic |
| ATTAGAGGGTCCAAATTTAG+AGG | 0.451507 | 3.4:-12674852 | None:intergenic |
| ATGGGGATGCTCGCCGTTGA+AGG | 0.452381 | 3.4:+12676242 | MS.gene85478:CDS |
| GGTATTAGAGTTAAGAGTGT+TGG | 0.475847 | 3.4:+12674809 | MS.gene85478:CDS |
| ATGGACGGACAGCGTTGGAT+AGG | 0.482442 | 3.4:+12676408 | MS.gene85478:CDS |
| GATCAAAATCATCACGAGAC+TGG | 0.494815 | 3.4:-12674949 | None:intergenic |
| CCGAGAAGAAGACAAGTTTG+TGG | 0.501506 | 3.4:-12674780 | None:intergenic |
| TTTATCATATGTTCCTTCAA+CGG | 0.514924 | 3.4:-12676255 | None:intergenic |
| TAGAATTTGAAATGATGGGT+TGG | 0.518105 | 3.4:-12674730 | None:intergenic |
| AGGTGACTTGCCCAAAATTG+AGG | 0.527850 | 3.4:+12676343 | MS.gene85478:CDS |
| TGGACGGACAGCGTTGGATA+GGG | 0.531515 | 3.4:+12676409 | MS.gene85478:CDS |
| ATGGGTCAAGATTCACATGC+TGG | 0.548494 | 3.4:+12674873 | MS.gene85478:CDS |
| GATTTAGAATTTGAAATGAT+GGG | 0.552146 | 3.4:-12674734 | None:intergenic |
| TGCAGTACTTCAACTATATG+GGG | 0.563695 | 3.4:+12676225 | MS.gene85478:intron |
| ATTGAGGAACTCTTGAAAGC+GGG | 0.563783 | 3.4:+12676359 | MS.gene85478:CDS |
| GGTGTTGATGCTGAAGGTGC+TGG | 0.568178 | 3.4:+12674914 | MS.gene85478:CDS |
| CGCAGATGGACGGACAGCGT+TGG | 0.577773 | 3.4:+12676403 | MS.gene85478:CDS |
| AACAACAGTAGAATGTCCAC+GGG | 0.593704 | 3.4:-12676308 | None:intergenic |
| ATGTAAAAGACGCAGATGGA+CGG | 0.600271 | 3.4:+12676393 | MS.gene85478:CDS |
| TAACAACAGTAGAATGTCCA+CGG | 0.603710 | 3.4:-12676309 | None:intergenic |
| TATGATGTAAAAGACGCAGA+TGG | 0.606140 | 3.4:+12676389 | MS.gene85478:CDS |
| GTGAATCTTGACCCATTAGA+GGG | 0.615376 | 3.4:-12674866 | None:intergenic |
| AATTGAGGAACTCTTGAAAG+CGG | 0.636086 | 3.4:+12676358 | MS.gene85478:CDS |
| TTAGAGTTAAGAGTGTTGGA+TGG | 0.638432 | 3.4:+12674813 | MS.gene85478:CDS |
| CTGTTGTTAGCTGCATCCGA+AGG | 0.640380 | 3.4:+12676323 | MS.gene85478:CDS |
| AGGAACATATGATAAAATGG+AGG | 0.671636 | 3.4:+12676262 | MS.gene85478:CDS |
| AAGCCAAAACATTCACCCCG+TGG | 0.724887 | 3.4:+12676292 | MS.gene85478:CDS |
| ACAACAGTAGAATGTCCACG+GGG | 0.853016 | 3.4:-12676307 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATAAATTTTTTATTGGTT+TGG | - | chr3.4:12676189-12676208 | None:intergenic | 10.0% |
| !!! | ATTTTTTAAAAAACAATTTG+AGG | - | chr3.4:12675572-12675591 | None:intergenic | 10.0% |
| !!! | TGTAGATATAAATTTTTTAT+TGG | - | chr3.4:12676194-12676213 | None:intergenic | 10.0% |
| !! | ATTTAGTGTTATATTGTATT+GGG | - | chr3.4:12675115-12675134 | None:intergenic | 15.0% |
| !! | TATTTAGTGTTATATTGTAT+TGG | - | chr3.4:12675116-12675135 | None:intergenic | 15.0% |
| !! | AAAAGTGGAATATATTGTAT+TGG | - | chr3.4:12675157-12675176 | None:intergenic | 20.0% |
| !! | AAAGTGGAATATATTGTATT+GGG | - | chr3.4:12675156-12675175 | None:intergenic | 20.0% |
| !! | GTGAATGAGTTATTAAAAAA+GGG | - | chr3.4:12675386-12675405 | None:intergenic | 20.0% |
| !! | TAAAAAACAATTTGAGGAAA+GGG | - | chr3.4:12675566-12675585 | None:intergenic | 20.0% |
| !! | TGAATGAGTTATTAAAAAAG+GGG | - | chr3.4:12675385-12675404 | None:intergenic | 20.0% |
| !! | TGTGAATGAGTTATTAAAAA+AGG | - | chr3.4:12675387-12675406 | None:intergenic | 20.0% |
| !! | TTAAAAAACAATTTGAGGAA+AGG | - | chr3.4:12675567-12675586 | None:intergenic | 20.0% |
| !!! | AAGAAAAGTGTATAAATGAA+GGG | - | chr3.4:12675180-12675199 | None:intergenic | 20.0% |
| !!! | AATTTTAACTTGTGTAACTA+TGG | + | chr3.4:12675721-12675740 | MS.gene85478:intron | 20.0% |
| !!! | ATTTGTTCTGTTCTTTTATT+AGG | + | chr3.4:12675650-12675669 | MS.gene85478:intron | 20.0% |
| !!! | ATTTTAACTTGTGTAACTAT+GGG | + | chr3.4:12675722-12675741 | MS.gene85478:intron | 20.0% |
| !!! | GACACTTTTTTTTAGAAAAT+GGG | - | chr3.4:12675046-12675065 | None:intergenic | 20.0% |
| !!! | GATTTAGAATTTGAAATGAT+GGG | - | chr3.4:12674737-12674756 | None:intergenic | 20.0% |
| !!! | TAAGAAAAGTGTATAAATGA+AGG | - | chr3.4:12675181-12675200 | None:intergenic | 20.0% |
| !!! | TTTAAAATGCAGTAACAAAA+TGG | - | chr3.4:12675325-12675344 | None:intergenic | 20.0% |
| ! | AAAAAACAATTTGAGGAAAG+GGG | - | chr3.4:12675565-12675584 | None:intergenic | 25.0% |
| ! | ATGTTTCATGAAACTGAAAA+TGG | - | chr3.4:12675834-12675853 | None:intergenic | 25.0% |
| ! | TGAAGGAACATATGATAAAA+TGG | + | chr3.4:12676259-12676278 | MS.gene85478:CDS | 25.0% |
| ! | TTTATCATATGTTCCTTCAA+CGG | - | chr3.4:12676258-12676277 | None:intergenic | 25.0% |
| !! | ATTTTCTGTTTGCAAAATGA+AGG | - | chr3.4:12676009-12676028 | None:intergenic | 25.0% |
| !! | GGATTTAGAATTTGAAATGA+TGG | - | chr3.4:12674738-12674757 | None:intergenic | 25.0% |
| !! | TGAAATGAGTCAGTAATTTT+GGG | - | chr3.4:12675068-12675087 | None:intergenic | 25.0% |
| !! | TTAAAATTTTCCTTGCAACT+AGG | - | chr3.4:12675709-12675728 | None:intergenic | 25.0% |
| !! | TTGAAATGAGTCAGTAATTT+TGG | - | chr3.4:12675069-12675088 | None:intergenic | 25.0% |
| !! | TTTTGCAAACAGAAAATATC+AGG | + | chr3.4:12676012-12676031 | MS.gene85478:intron | 25.0% |
| !!! | AGTTAGTGTCACTTTAAATT+AGG | + | chr3.4:12675530-12675549 | MS.gene85478:intron | 25.0% |
| !!! | CACAAAAACTACTTTTTAGT+AGG | - | chr3.4:12675442-12675461 | None:intergenic | 25.0% |
| !!! | GGACACTTTTTTTTAGAAAA+TGG | - | chr3.4:12675047-12675066 | None:intergenic | 25.0% |
| !!! | GTTAGTGTCACTTTAAATTA+GGG | + | chr3.4:12675531-12675550 | MS.gene85478:intron | 25.0% |
| !!! | TTAGTGTCACTTTAAATTAG+GGG | + | chr3.4:12675532-12675551 | MS.gene85478:intron | 25.0% |
| !!! | TTGTTACTGCATTTTAAAAG+TGG | + | chr3.4:12675327-12675346 | MS.gene85478:intron | 25.0% |
| AGAAAATGGGTCATTTATTC+CGG | - | chr3.4:12675033-12675052 | None:intergenic | 30.0% | |
| AGAAACTTGTGAAGTGATTA+AGG | - | chr3.4:12674759-12674778 | None:intergenic | 30.0% | |
| AGGAACATATGATAAAATGG+AGG | + | chr3.4:12676262-12676281 | MS.gene85478:CDS | 30.0% | |
| ATATCAGGAGTAAATCTAGA+AGG | + | chr3.4:12676027-12676046 | MS.gene85478:intron | 30.0% | |
| TGATTGTGCTTATGTATTAC+TGG | + | chr3.4:12675745-12675764 | MS.gene85478:intron | 30.0% | |
| TGTATAAATGAAGGGAAAAG+TGG | - | chr3.4:12675172-12675191 | None:intergenic | 30.0% | |
| TTGCAGTACTTCAACTATAT+GGG | + | chr3.4:12676224-12676243 | MS.gene85478:intron | 30.0% | |
| TTTGCAGTACTTCAACTATA+TGG | + | chr3.4:12676223-12676242 | MS.gene85478:intron | 30.0% | |
| ! | AGGGAGTAATAAAGTTTGTT+TGG | - | chr3.4:12675005-12675024 | None:intergenic | 30.0% |
| ! | TGGTTCAAGTTTGGTAAAAA+TGG | + | chr3.4:12674893-12674912 | MS.gene85478:CDS | 30.0% |
| ! | TTCAAGAGTTCCTCAATTTT+GGG | - | chr3.4:12676356-12676375 | None:intergenic | 30.0% |
| ! | TTTCAAGAGTTCCTCAATTT+TGG | - | chr3.4:12676357-12676376 | None:intergenic | 30.0% |
| !! | CATAGTTTTGCTTTTGATTC+TGG | + | chr3.4:12675610-12675629 | MS.gene85478:intron | 30.0% |
| !! | TAGAATTTGAAATGATGGGT+TGG | - | chr3.4:12674733-12674752 | None:intergenic | 30.0% |
| AATTGAGGAACTCTTGAAAG+CGG | + | chr3.4:12676358-12676377 | MS.gene85478:CDS | 35.0% | |
| ATTAGAGGGTCCAAATTTAG+AGG | - | chr3.4:12674855-12674874 | None:intergenic | 35.0% | |
| CAAAGTAGGTCAATTATACC+GGG | + | chr3.4:12675216-12675235 | MS.gene85478:intron | 35.0% | |
| CAGATGAGTTAATCTTCTCA+TGG | - | chr3.4:12676133-12676152 | None:intergenic | 35.0% | |
| CCAAAGTAGGTCAATTATAC+CGG | + | chr3.4:12675215-12675234 | MS.gene85478:intron | 35.0% | |
| CTAAATTTGGACCCTCTAAT+GGG | + | chr3.4:12674855-12674874 | MS.gene85478:CDS | 35.0% | |
| GAAAATGGGTCATTTATTCC+GGG | - | chr3.4:12675032-12675051 | None:intergenic | 35.0% | |
| GATTCAAATTCCGTCTCTTA+AGG | - | chr3.4:12675495-12675514 | None:intergenic | 35.0% | |
| GGAGTTTCTAATAACCGATA+AGG | + | chr3.4:12675671-12675690 | MS.gene85478:intron | 35.0% | |
| GGATCATTCTCATTCCTTAT+CGG | - | chr3.4:12675688-12675707 | None:intergenic | 35.0% | |
| TAACAACAGTAGAATGTCCA+CGG | - | chr3.4:12676312-12676331 | None:intergenic | 35.0% | |
| TATGATGTAAAAGACGCAGA+TGG | + | chr3.4:12676389-12676408 | MS.gene85478:CDS | 35.0% | |
| TCAAGCTTCACCTCTAAATT+TGG | + | chr3.4:12674842-12674861 | MS.gene85478:CDS | 35.0% | |
| TCTAAATTTGGACCCTCTAA+TGG | + | chr3.4:12674854-12674873 | MS.gene85478:CDS | 35.0% | |
| TCTGTGTGAAAAACCAAAGT+AGG | + | chr3.4:12675202-12675221 | MS.gene85478:intron | 35.0% | |
| TGCAGTACTTCAACTATATG+GGG | + | chr3.4:12676225-12676244 | MS.gene85478:intron | 35.0% | |
| ! | GGTATTAGAGTTAAGAGTGT+TGG | + | chr3.4:12674809-12674828 | MS.gene85478:CDS | 35.0% |
| ! | TAGTTGCTTATGTTTTGCGT+TGG | + | chr3.4:12675923-12675942 | MS.gene85478:intron | 35.0% |
| !! | AAAAATGGTGTTGATGCTGA+AGG | + | chr3.4:12674908-12674927 | MS.gene85478:CDS | 35.0% |
| !! | TTAGAGTTAAGAGTGTTGGA+TGG | + | chr3.4:12674813-12674832 | MS.gene85478:CDS | 35.0% |
| AACAACAGTAGAATGTCCAC+GGG | - | chr3.4:12676311-12676330 | None:intergenic | 40.0% | |
| ATGTAAAAGACGCAGATGGA+CGG | + | chr3.4:12676393-12676412 | MS.gene85478:CDS | 40.0% | |
| ATTGAGGAACTCTTGAAAGC+GGG | + | chr3.4:12676359-12676378 | MS.gene85478:CDS | 40.0% | |
| CCACAAACTTGTCTTCTTCT+CGG | + | chr3.4:12674780-12674799 | MS.gene85478:CDS | 40.0% | |
| CCGGTATAATTGACCTACTT+TGG | - | chr3.4:12675218-12675237 | None:intergenic | 40.0% | |
| GACTCTGTAACCTTAAGAGA+CGG | + | chr3.4:12675482-12675501 | MS.gene85478:intron | 40.0% | |
| GATCAAAATCATCACGAGAC+TGG | - | chr3.4:12674952-12674971 | None:intergenic | 40.0% | |
| GTGAATCTTGACCCATTAGA+GGG | - | chr3.4:12674869-12674888 | None:intergenic | 40.0% | |
| TGAGAATGATCCTAGTTGCA+AGG | + | chr3.4:12675696-12675715 | MS.gene85478:intron | 40.0% | |
| TGATCGAGATGATGTTGAAC+AGG | + | chr3.4:12674967-12674986 | MS.gene85478:CDS | 40.0% | |
| TGTGAATCTTGACCCATTAG+AGG | - | chr3.4:12674870-12674889 | None:intergenic | 40.0% | |
| TTGCAAAATGAAGGACGACT+CGG | - | chr3.4:12676000-12676019 | None:intergenic | 40.0% | |
| TTGTCTTCTTCTCGGTTTCT+TGG | + | chr3.4:12674788-12674807 | MS.gene85478:CDS | 40.0% | |
| ! | AATTTTGGGCAAGTCACCTT+CGG | - | chr3.4:12676342-12676361 | None:intergenic | 40.0% |
| ! | ACTGCATTTTCCTTGTTGCA+TGG | + | chr3.4:12675359-12675378 | MS.gene85478:intron | 40.0% |
| ! | CTCGGTGATAATTTTGAGCT+GGG | - | chr3.4:12675982-12676001 | None:intergenic | 40.0% |
| ! | GTAGTTTTTGTGTACGAGTG+TGG | + | chr3.4:12675450-12675469 | MS.gene85478:intron | 40.0% |
| !! | ACTCGGTGATAATTTTGAGC+TGG | - | chr3.4:12675983-12676002 | None:intergenic | 40.0% |
| !! | TTCACATGCTGGTTCAAGTT+TGG | + | chr3.4:12674884-12674903 | MS.gene85478:CDS | 40.0% |
| AAAAAAGGGGCCATGCAACA+AGG | - | chr3.4:12675372-12675391 | None:intergenic | 45.0% | |
| ACAACAGTAGAATGTCCACG+GGG | - | chr3.4:12676310-12676329 | None:intergenic | 45.0% | |
| ATGGGTCAAGATTCACATGC+TGG | + | chr3.4:12674873-12674892 | MS.gene85478:CDS | 45.0% | |
| ATGGGTCATTTATTCCGGGA+CGG | - | chr3.4:12675028-12675047 | None:intergenic | 45.0% | |
| CCGAGAAGAAGACAAGTTTG+TGG | - | chr3.4:12674783-12674802 | None:intergenic | 45.0% | |
| GTAGGTCAATTATACCGGGA+CGG | + | chr3.4:12675220-12675239 | MS.gene85478:intron | 45.0% | |
| ! | AGGTGACTTGCCCAAAATTG+AGG | + | chr3.4:12676343-12676362 | MS.gene85478:CDS | 45.0% |
| !! | GCTGAAGGTGCTGGTATTTA+TGG | + | chr3.4:12674923-12674942 | MS.gene85478:CDS | 45.0% |
| AACAAATACTCCCTCCGTCC+CGG | - | chr3.4:12675237-12675256 | None:intergenic | 50.0% | |
| AAGCCAAAACATTCACCCCG+TGG | + | chr3.4:12676292-12676311 | MS.gene85478:CDS | 50.0% | |
| CTGTTGTTAGCTGCATCCGA+AGG | + | chr3.4:12676323-12676342 | MS.gene85478:CDS | 50.0% | |
| CTTTATTACTCCCTCCGTCC+CGG | + | chr3.4:12675011-12675030 | MS.gene85478:intron | 50.0% | |
| GTCAATTATACCGGGACGGA+GGG | + | chr3.4:12675224-12675243 | MS.gene85478:intron | 50.0% | |
| GTCATTTATTCCGGGACGGA+GGG | - | chr3.4:12675024-12675043 | None:intergenic | 50.0% | |
| ! | TGTCCACGGGGTGAATGTTT+TGG | - | chr3.4:12676298-12676317 | None:intergenic | 50.0% |
| GGTCAATTATACCGGGACGG+AGG | + | chr3.4:12675223-12675242 | MS.gene85478:intron | 55.0% | |
| GGTCATTTATTCCGGGACGG+AGG | - | chr3.4:12675025-12675044 | None:intergenic | 55.0% | |
| ! | ATGGACGGACAGCGTTGGAT+AGG | + | chr3.4:12676408-12676427 | MS.gene85478:CDS | 55.0% |
| ! | TGGACGGACAGCGTTGGATA+GGG | + | chr3.4:12676409-12676428 | MS.gene85478:CDS | 55.0% |
| !! | GGTGTTGATGCTGAAGGTGC+TGG | + | chr3.4:12674914-12674933 | MS.gene85478:CDS | 55.0% |
| ATGGGGATGCTCGCCGTTGA+AGG | + | chr3.4:12676242-12676261 | MS.gene85478:CDS | 60.0% | |
| CGCAGATGGACGGACAGCGT+TGG | + | chr3.4:12676403-12676422 | MS.gene85478:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 12674704 | 12676484 | 12674704 | ID=MS.gene85478 |
| chr3.4 | mRNA | 12674704 | 12676484 | 12674704 | ID=MS.gene85478.t1;Parent=MS.gene85478 |
| chr3.4 | exon | 12674704 | 12674988 | 12674704 | ID=MS.gene85478.t1.exon1;Parent=MS.gene85478.t1 |
| chr3.4 | CDS | 12674704 | 12674988 | 12674704 | ID=cds.MS.gene85478.t1;Parent=MS.gene85478.t1 |
| chr3.4 | exon | 12676230 | 12676484 | 12676230 | ID=MS.gene85478.t1.exon2;Parent=MS.gene85478.t1 |
| chr3.4 | CDS | 12676230 | 12676484 | 12676230 | ID=cds.MS.gene85478.t1;Parent=MS.gene85478.t1 |
| Gene Sequence |
| Protein sequence |
