Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022141.01.T01 | MCH81247.1 | 80.952 | 84 | 11 | 1 | 1 | 84 | 8 | 86 | 5.20E-42 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022141.01.T01 | Q0JKX1 | 79.688 | 64 | 13 | 0 | 1 | 64 | 137 | 200 | 4.37E-31 | 111 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022141.01.T01 | A0A392M1M8 | 80.952 | 84 | 11 | 1 | 1 | 84 | 8 | 86 | 2.49e-42 | 142 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048889.01 | MsG0480022141.01 | 0.835141 | 1.994498e-56 | 2.417154e-53 |
| MsG0080049023.01 | MsG0480022141.01 | -0.804225 | 2.392029e-49 | 1.226566e-46 |
| MsG0080049038.01 | MsG0480022141.01 | -0.831729 | 1.415239e-55 | 1.549322e-52 |
| MsG0080049066.01 | MsG0480022141.01 | -0.821847 | 3.248480e-53 | 2.673569e-50 |
| MsG0080049133.01 | MsG0480022141.01 | -0.806241 | 9.049251e-50 | 4.887313e-47 |
| MsG0180000367.01 | MsG0480022141.01 | 0.802020 | 6.838825e-49 | 3.314487e-46 |
| MsG0180000423.01 | MsG0480022141.01 | -0.802964 | 4.368480e-49 | 2.168758e-46 |
| MsG0180000719.01 | MsG0480022141.01 | -0.814528 | 1.471567e-51 | 9.898373e-49 |
| MsG0180001054.01 | MsG0480022141.01 | -0.802977 | 4.341943e-49 | 2.156218e-46 |
| MsG0180001072.01 | MsG0480022141.01 | -0.800121 | 1.671758e-48 | 7.721822e-46 |
| MsG0180001614.01 | MsG0480022141.01 | -0.821849 | 3.244101e-53 | 2.670205e-50 |
| MsG0180001925.01 | MsG0480022141.01 | -0.803274 | 3.770036e-49 | 1.886525e-46 |
| MsG0180002099.01 | MsG0480022141.01 | -0.813211 | 2.871518e-51 | 1.863549e-48 |
| MsG0180002809.01 | MsG0480022141.01 | -0.823611 | 1.262091e-53 | 1.092032e-50 |
| MsG0180003114.01 | MsG0480022141.01 | -0.805757 | 1.144357e-49 | 6.104453e-47 |
| MsG0180003758.01 | MsG0480022141.01 | -0.816696 | 4.841151e-52 | 3.454529e-49 |
| MsG0180003908.01 | MsG0480022141.01 | -0.809357 | 1.969311e-50 | 1.153817e-47 |
| MsG0180004322.01 | MsG0480022141.01 | -0.805337 | 1.401814e-49 | 7.396946e-47 |
| MsG0180004580.01 | MsG0480022141.01 | -0.808037 | 3.770248e-50 | 2.133037e-47 |
| MsG0180004581.01 | MsG0480022141.01 | -0.824980 | 6.014922e-54 | 5.411860e-51 |
| MsG0180004737.01 | MsG0480022141.01 | -0.807671 | 4.509585e-50 | 2.526995e-47 |
| MsG0180004787.01 | MsG0480022141.01 | -0.851188 | 1.044502e-60 | 2.097189e-57 |
| MsG0180005369.01 | MsG0480022141.01 | -0.826010 | 3.429550e-54 | 3.177465e-51 |
| MsG0180005817.01 | MsG0480022141.01 | -0.806815 | 6.849489e-50 | 3.753887e-47 |
| MsG0180005848.01 | MsG0480022141.01 | -0.822479 | 2.317040e-53 | 1.941691e-50 |
| MsG0180006212.01 | MsG0480022141.01 | -0.826068 | 3.322010e-54 | 3.082732e-51 |
| MsG0280006381.01 | MsG0480022141.01 | -0.817631 | 2.983208e-52 | 2.183998e-49 |
| MsG0280011050.01 | MsG0480022141.01 | 0.838427 | 2.895356e-57 | 3.876691e-54 |
| MsG0280011269.01 | MsG0480022141.01 | 0.804392 | 2.208253e-49 | 1.137214e-46 |
| MsG0280011299.01 | MsG0480022141.01 | -0.824777 | 6.714308e-54 | 6.006228e-51 |
| MsG0280011475.01 | MsG0480022141.01 | -0.831938 | 1.256892e-55 | 1.384440e-52 |
| MsG0380012157.01 | MsG0480022141.01 | -0.810361 | 1.197812e-50 | 7.205441e-48 |
| MsG0380012281.01 | MsG0480022141.01 | -0.829259 | 5.690370e-55 | 5.794133e-52 |
| MsG0380013473.01 | MsG0480022141.01 | -0.813861 | 2.065604e-51 | 1.364482e-48 |
| MsG0380014231.01 | MsG0480022141.01 | -0.801574 | 8.443539e-49 | 4.046206e-46 |
| MsG0380014642.01 | MsG0480022141.01 | -0.817827 | 2.694079e-52 | 1.983056e-49 |
| MsG0380014735.01 | MsG0480022141.01 | -0.833348 | 5.614253e-56 | 6.448726e-53 |
| MsG0480018463.01 | MsG0480022141.01 | -0.801014 | 1.099737e-48 | 5.196383e-46 |
| MsG0480019183.01 | MsG0480022141.01 | -0.842416 | 2.620314e-58 | 3.970696e-55 |
| MsG0480019861.01 | MsG0480022141.01 | -0.805958 | 1.038181e-49 | 5.566533e-47 |
| MsG0480020938.01 | MsG0480022141.01 | -0.816219 | 6.191056e-52 | 4.360797e-49 |
| MsG0480020958.01 | MsG0480022141.01 | 0.824766 | 6.755952e-54 | 6.041581e-51 |
| MsG0480021175.01 | MsG0480022141.01 | 0.820367 | 7.120584e-53 | 5.623185e-50 |
| MsG0480021226.01 | MsG0480022141.01 | 0.812296 | 4.554111e-51 | 2.884595e-48 |
| MsG0480021912.01 | MsG0480022141.01 | -0.818654 | 1.751424e-52 | 1.318759e-49 |
| MsG0480022141.01 | MsG0480022142.01 | 0.870280 | 1.644413e-66 | 6.360875e-63 |
| MsG0480022141.01 | MsG0480022428.01 | 0.803333 | 3.664851e-49 | 1.836668e-46 |
| MsG0480022141.01 | MsG0480022687.01 | -0.803146 | 4.006818e-49 | 1.998431e-46 |
| MsG0480022141.01 | MsG0480023067.01 | 0.857242 | 1.865314e-62 | 4.573573e-59 |
| MsG0480022141.01 | MsG0480023103.01 | -0.805251 | 1.461266e-49 | 7.693473e-47 |
| MsG0480022141.01 | MsG0480023125.01 | 0.810134 | 1.340583e-50 | 8.016548e-48 |
| MsG0480022141.01 | MsG0480023152.01 | -0.837175 | 6.071137e-57 | 7.822624e-54 |
| MsG0480022141.01 | MsG0480023242.01 | -0.811311 | 7.459504e-51 | 4.601806e-48 |
| MsG0480022141.01 | MsG0480023244.01 | -0.818001 | 2.461873e-52 | 1.820739e-49 |
| MsG0480022141.01 | MsG0480023695.01 | -0.818417 | 1.981756e-52 | 1.482695e-49 |
| MsG0480022141.01 | MsG0580024135.01 | -0.808156 | 3.557069e-50 | 2.018886e-47 |
| MsG0480022141.01 | MsG0580024278.01 | -0.805842 | 1.098368e-49 | 5.871671e-47 |
| MsG0480022141.01 | MsG0580024309.01 | -0.809127 | 2.206431e-50 | 1.284786e-47 |
| MsG0480022141.01 | MsG0580024493.01 | -0.833240 | 5.974110e-56 | 6.839363e-53 |
| MsG0480022141.01 | MsG0580024678.01 | -0.809281 | 2.044559e-50 | 1.195434e-47 |
| MsG0480022141.01 | MsG0580025105.01 | -0.820685 | 6.020029e-53 | 4.795707e-50 |
| MsG0480022141.01 | MsG0580025117.01 | 0.830529 | 2.790590e-55 | 2.947747e-52 |
| MsG0480022141.01 | MsG0580025486.01 | -0.827134 | 1.850221e-54 | 1.770353e-51 |
| MsG0480022141.01 | MsG0580026362.01 | -0.810401 | 1.173764e-50 | 7.068166e-48 |
| MsG0480022141.01 | MsG0580027276.01 | -0.802865 | 4.580131e-49 | 2.268141e-46 |
| MsG0480022141.01 | MsG0580027325.01 | -0.800801 | 1.215390e-48 | 5.711405e-46 |
| MsG0480022141.01 | MsG0580028159.01 | -0.817444 | 3.287696e-52 | 2.394730e-49 |
| MsG0480022141.01 | MsG0580029177.01 | -0.806949 | 6.414396e-50 | 3.527633e-47 |
| MsG0480022141.01 | MsG0580029623.01 | 0.839350 | 1.670188e-57 | 2.300370e-54 |
| MsG0480022141.01 | MsG0580029792.01 | -0.826230 | 3.039996e-54 | 2.834215e-51 |
| MsG0480022141.01 | MsG0580030019.01 | 0.828113 | 1.076514e-54 | 1.059999e-51 |
| MsG0480022141.01 | MsG0580030237.01 | -0.806571 | 7.709976e-50 | 4.199005e-47 |
| MsG0480022141.01 | MsG0680030635.01 | -0.822425 | 2.385485e-53 | 1.996034e-50 |
| MsG0480022141.01 | MsG0680031062.01 | 0.820831 | 5.572835e-53 | 4.457965e-50 |
| MsG0480022141.01 | MsG0680034139.01 | 0.844123 | 9.183451e-59 | 1.468095e-55 |
| MsG0480022141.01 | MsG0680035584.01 | -0.816690 | 4.857079e-52 | 3.465255e-49 |
| MsG0480022141.01 | MsG0680035870.01 | -0.825498 | 4.537686e-54 | 4.142954e-51 |
| MsG0480022141.01 | MsG0780036054.01 | 0.807741 | 4.357856e-50 | 2.446553e-47 |
| MsG0480022141.01 | MsG0780037458.01 | -0.804370 | 2.232130e-49 | 1.148890e-46 |
| MsG0480022141.01 | MsG0780038047.01 | -0.815768 | 7.804229e-52 | 5.429990e-49 |
| MsG0480022141.01 | MsG0780038305.01 | 0.842798 | 2.075437e-58 | 3.182070e-55 |
| MsG0480022141.01 | MsG0780039431.01 | 0.832010 | 1.206269e-55 | 1.331417e-52 |
| MsG0480022141.01 | MsG0780039821.01 | -0.803264 | 3.788276e-49 | 1.895168e-46 |
| MsG0480022141.01 | MsG0780039836.01 | -0.815107 | 1.095070e-51 | 7.481423e-49 |
| MsG0480022141.01 | MsG0780039838.01 | -0.800002 | 1.767482e-48 | 8.139339e-46 |
| MsG0480022141.01 | MsG0780039839.01 | -0.812520 | 4.068844e-51 | 2.592673e-48 |
| MsG0480022141.01 | MsG0780040121.01 | -0.809163 | 2.166842e-50 | 1.262953e-47 |
| MsG0480022141.01 | MsG0780040266.01 | 0.805539 | 1.271483e-49 | 6.744090e-47 |
| MsG0480022141.01 | MsG0780040685.01 | -0.809331 | 1.995331e-50 | 1.168182e-47 |
| MsG0480022141.01 | MsG0780040892.01 | -0.803308 | 3.709115e-49 | 1.857632e-46 |
| MsG0480022141.01 | MsG0780040977.01 | 0.816856 | 4.456072e-52 | 3.193907e-49 |
| MsG0480022141.01 | MsG0780041531.01 | -0.850051 | 2.180216e-60 | 4.217291e-57 |
| MsG0480022141.01 | MsG0780041620.01 | -0.815723 | 7.989018e-52 | 5.551511e-49 |
| MsG0480022141.01 | MsG0780041637.01 | -0.822646 | 2.118825e-53 | 1.784130e-50 |
| MsG0480022141.01 | MsG0780041720.01 | -0.827724 | 1.335983e-54 | 1.300528e-51 |
| MsG0480022141.01 | MsG0780041783.01 | -0.822053 | 2.909588e-53 | 2.408583e-50 |
| MsG0480022141.01 | MsG0880041908.01 | -0.809713 | 1.651468e-50 | 9.767692e-48 |
| MsG0480022141.01 | MsG0880042122.01 | 0.804680 | 1.922971e-49 | 9.977175e-47 |
| MsG0480022141.01 | MsG0880042588.01 | -0.806082 | 9.776508e-50 | 5.258492e-47 |
| MsG0480022141.01 | MsG0880043261.01 | 0.829994 | 3.769292e-55 | 3.920719e-52 |
| MsG0480022141.01 | MsG0880044339.01 | -0.800548 | 1.368817e-48 | 6.391308e-46 |
| MsG0480022141.01 | MsG0880044548.01 | -0.813040 | 3.130359e-51 | 2.022226e-48 |
| MsG0480022141.01 | MsG0880045188.01 | -0.814200 | 1.738722e-51 | 1.159136e-48 |
| MsG0480022141.01 | MsG0880045369.01 | -0.813648 | 2.300748e-51 | 1.511107e-48 |
| MsG0480022141.01 | MsG0880045385.01 | -0.827296 | 1.692121e-54 | 1.626912e-51 |
| MsG0480022141.01 | MsG0880045413.01 | -0.818583 | 1.817475e-52 | 1.365846e-49 |
| MsG0480022141.01 | MsG0880045414.01 | -0.801259 | 9.796617e-49 | 4.657486e-46 |
| MsG0480022141.01 | MsG0880045703.01 | -0.817873 | 2.631119e-52 | 1.939106e-49 |
| MsG0480022141.01 | MsG0880045804.01 | 0.801421 | 9.074443e-49 | 4.331798e-46 |
| MsG0480022141.01 | MsG0880045808.01 | 0.801285 | 9.675988e-49 | 4.603195e-46 |
| MsG0480022141.01 | MsG0880045968.01 | -0.848495 | 5.911989e-60 | 1.087045e-56 |
| MsG0480022141.01 | MsG0880046010.01 | -0.820203 | 7.766491e-53 | 6.105432e-50 |
| MsG0480022141.01 | MsG0880046151.01 | -0.809952 | 1.467213e-50 | 8.732459e-48 |
| MsG0480022141.01 | MsG0880046167.01 | -0.818455 | 1.942885e-52 | 1.455134e-49 |
| MsG0480022141.01 | MsG0880046219.01 | -0.809112 | 2.223021e-50 | 1.293901e-47 |
| MsG0480022141.01 | MsG0880046341.01 | -0.827365 | 1.628695e-54 | 1.569259e-51 |
| MsG0480022141.01 | MsG0880046469.01 | -0.827645 | 1.395236e-54 | 1.354990e-51 |
| MsG0480022141.01 | MsG0880046838.01 | -0.828773 | 7.462418e-55 | 7.489260e-52 |
| MsG0480022141.01 | MsG0880047318.01 | -0.826169 | 3.143590e-54 | 2.925633e-51 |
| MsG0480022141.01 | MsG0880047493.01 | 0.816032 | 6.815270e-52 | 4.775947e-49 |
| MsG0480022141.01 | MsG0880047714.01 | 0.802820 | 4.679045e-49 | 2.314582e-46 |
| MsG0380015271.01 | MsG0480022141.01 | -0.812179 | 4.828493e-51 | 3.048743e-48 |
| MsG0380015752.01 | MsG0480022141.01 | -0.814752 | 1.312759e-51 | 8.883253e-49 |
| MsG0380016056.01 | MsG0480022141.01 | -0.805285 | 1.437384e-49 | 7.574428e-47 |
| MsG0380016166.01 | MsG0480022141.01 | -0.805957 | 1.038817e-49 | 5.569756e-47 |
| MsG0380016229.01 | MsG0480022141.01 | -0.837557 | 4.846170e-57 | 6.318062e-54 |
| MsG0380016476.01 | MsG0480022141.01 | 0.803384 | 3.577094e-49 | 1.795033e-46 |
| MsG0380016835.01 | MsG0480022141.01 | -0.802647 | 5.080304e-49 | 2.501804e-46 |
| MsG0380016889.01 | MsG0480022141.01 | -0.835957 | 1.239798e-56 | 1.539838e-53 |
| MsG0380016934.01 | MsG0480022141.01 | -0.822593 | 2.180003e-53 | 1.832965e-50 |
| MsG0380016935.01 | MsG0480022141.01 | -0.824178 | 9.293165e-54 | 8.170637e-51 |
| MsG0380016960.01 | MsG0480022141.01 | -0.801805 | 7.570770e-49 | 3.649250e-46 |
| MsG0380017231.01 | MsG0480022141.01 | 0.817681 | 2.906483e-52 | 2.130799e-49 |
| MsG0380017405.01 | MsG0480022141.01 | -0.821685 | 3.541227e-53 | 2.901073e-50 |
| MsG0380017406.01 | MsG0480022141.01 | -0.800355 | 1.498077e-48 | 6.961019e-46 |
| MsG0380017928.01 | MsG0480022141.01 | -0.838339 | 3.050176e-57 | 4.072881e-54 |
| MsG0380017962.01 | MsG0480022141.01 | -0.803685 | 3.098052e-49 | 1.566740e-46 |
| MsG0480018112.01 | MsG0480022141.01 | -0.803521 | 3.351318e-49 | 1.687747e-46 |
| MsG0480018201.01 | MsG0480022141.01 | -0.817543 | 3.123582e-52 | 2.281333e-49 |
| MsG0480018202.01 | MsG0480022141.01 | -0.802766 | 4.800225e-49 | 2.371235e-46 |
| MsG0480018203.01 | MsG0480022141.01 | -0.811236 | 7.743024e-51 | 4.767526e-48 |
| MsG0280007786.01 | MsG0480022141.01 | 0.834240 | 3.360379e-56 | 3.964201e-53 |
| MsG0280008697.01 | MsG0480022141.01 | 0.802040 | 6.775008e-49 | 3.285200e-46 |
| MsG0280009079.01 | MsG0480022141.01 | -0.814590 | 1.426173e-51 | 9.608957e-49 |
| MsG0280009742.01 | MsG0480022141.01 | -0.815894 | 7.318087e-52 | 5.108501e-49 |
| MsG0280009864.01 | MsG0480022141.01 | -0.824047 | 9.971654e-54 | 8.734413e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022141.01.T01 | MTR_4g084750 | 95.890 | 73 | 3 | 0 | 1 | 73 | 124 | 196 | 5.99e-39 | 128 |
| MsG0480022141.01.T01 | MTR_2g061030 | 53.623 | 69 | 26 | 1 | 1 | 69 | 259 | 321 | 2.83e-17 | 74.3 |
| MsG0480022141.01.T01 | MTR_1g054285 | 55.556 | 63 | 22 | 1 | 1 | 63 | 213 | 269 | 8.92e-16 | 70.1 |
| MsG0480022141.01.T01 | MTR_4g097600 | 50.000 | 60 | 24 | 1 | 1 | 60 | 186 | 239 | 2.15e-14 | 66.2 |
| MsG0480022141.01.T01 | MTR_7g093430 | 53.333 | 60 | 22 | 1 | 1 | 60 | 219 | 272 | 2.53e-14 | 66.2 |
| MsG0480022141.01.T01 | MTR_5g013010 | 47.143 | 70 | 32 | 1 | 1 | 70 | 195 | 259 | 4.16e-14 | 65.5 |
| MsG0480022141.01.T01 | MTR_4g100550 | 48.571 | 70 | 31 | 1 | 1 | 70 | 179 | 243 | 9.05e-14 | 64.3 |
| MsG0480022141.01.T01 | MTR_5g014890 | 35.714 | 84 | 46 | 2 | 1 | 84 | 182 | 257 | 6.72e-13 | 62.0 |
| MsG0480022141.01.T01 | MTR_8g006705 | 50.847 | 59 | 24 | 1 | 1 | 59 | 205 | 258 | 1.03e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022141.01.T01 | AT2G01430 | 73.770 | 61 | 8 | 1 | 1 | 61 | 198 | 250 | 5.56e-24 | 91.7 |
| MsG0480022141.01.T01 | AT1G70920 | 65.574 | 61 | 8 | 1 | 1 | 61 | 98 | 145 | 6.69e-20 | 79.0 |
| MsG0480022141.01.T01 | AT1G70920 | 65.574 | 61 | 8 | 1 | 1 | 61 | 128 | 175 | 8.19e-20 | 79.3 |
| MsG0480022141.01.T01 | AT5G06710 | 48.810 | 84 | 35 | 2 | 1 | 82 | 249 | 326 | 1.45e-16 | 72.4 |
| MsG0480022141.01.T01 | AT2G44910 | 51.613 | 62 | 25 | 1 | 1 | 62 | 222 | 278 | 5.75e-14 | 65.5 |
| MsG0480022141.01.T01 | AT4G37790 | 43.333 | 60 | 28 | 1 | 1 | 60 | 185 | 238 | 1.54e-12 | 61.2 |
| MsG0480022141.01.T01 | AT3G60390 | 55.000 | 60 | 20 | 2 | 1 | 59 | 221 | 274 | 4.26e-12 | 60.1 |
| MsG0480022141.01.T01 | AT4G16780 | 50.820 | 61 | 25 | 2 | 1 | 61 | 188 | 243 | 5.84e-12 | 59.7 |
| MsG0480022141.01.T01 | AT5G47370 | 50.000 | 60 | 25 | 1 | 1 | 60 | 189 | 243 | 2.57e-11 | 57.8 |
| MsG0480022141.01.T01 | AT4G17460 | 50.000 | 60 | 25 | 1 | 1 | 60 | 194 | 248 | 2.86e-11 | 57.8 |
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGAAGTTGAGGAGCTT+AGG | 0.327770 | 4:-68809536 | MsG0480022141.01.T01:CDS |
| AGCGACTGTGGCGGTGGTAT+TGG | 0.370541 | 4:+68809413 | None:intergenic |
| AAGACCCAAACCACCTCTTC+AGG | 0.404339 | 4:+68809583 | None:intergenic |
| GGTGGTGACACGCTCACATC+GGG | 0.415895 | 4:+68809434 | None:intergenic |
| GGTCTTTAACTGAGCAAAAC+CGG | 0.425394 | 4:-68809566 | MsG0480022141.01.T01:CDS |
| AAGAGAAGTTGAGGAGCTTA+GGG | 0.432553 | 4:-68809535 | MsG0480022141.01.T01:CDS |
| CAACTTCTCTTTGTAGCCTC+CGG | 0.434325 | 4:+68809547 | None:intergenic |
| GAATACCTGAAGAGGTGGTT+TGG | 0.451281 | 4:-68809588 | MsG0480022141.01.T01:CDS |
| AATACCTGAAGAGGTGGTTT+GGG | 0.488971 | 4:-68809587 | MsG0480022141.01.T01:CDS |
| TTCGGCAGCGACTGTGGCGG+TGG | 0.489852 | 4:+68809407 | None:intergenic |
| CACATTGATAAGGTTGAAGC+TGG | 0.491305 | 4:+68809459 | None:intergenic |
| TGTCATGCTTGTCACACTCT+AGG | 0.496223 | 4:+68809354 | None:intergenic |
| GTCATGCTTGTCACACTCTA+GGG | 0.499070 | 4:+68809355 | None:intergenic |
| GGAGCTTAGGGCTATGAAGA+TGG | 0.505130 | 4:-68809523 | MsG0480022141.01.T01:CDS |
| GACTGTGGCGGTGGTATTGG+TGG | 0.515726 | 4:+68809416 | None:intergenic |
| GATAAGGTTGAAGCTGGAAG+AGG | 0.548416 | 4:+68809465 | None:intergenic |
| GAGCTTAGGGCTATGAAGAT+GGG | 0.567814 | 4:-68809522 | MsG0480022141.01.T01:CDS |
| ACATCGGGGACACATTGATA+AGG | 0.583310 | 4:+68809449 | None:intergenic |
| TGGTGGTGACACGCTCACAT+CGG | 0.609053 | 4:+68809433 | None:intergenic |
| CACTCTAGGGGACAAAATGA+CGG | 0.630061 | 4:+68809368 | None:intergenic |
| GAGGCTACAAAGAGAAGTTG+AGG | 0.631401 | 4:-68809544 | MsG0480022141.01.T01:CDS |
| TGGAGTGCGAATACCTGAAG+AGG | 0.632527 | 4:-68809596 | MsG0480022141.01.T01:CDS |
| TGAGGAGATAACACAGTAGG+TGG | 0.632854 | 4:+68809498 | None:intergenic |
| CTGTGAGGAGATAACACAGT+AGG | 0.640869 | 4:+68809495 | None:intergenic |
| TCATGCTTGTCACACTCTAG+GGG | 0.663913 | 4:+68809356 | None:intergenic |
| AGTGCGAATACCTGAAGAGG+TGG | 0.667742 | 4:-68809593 | MsG0480022141.01.T01:CDS |
| GGAAGAGGCTCAGAACTGTG+AGG | 0.688248 | 4:+68809480 | None:intergenic |
| CTTTAACTGAGCAAAACCGG+AGG | 0.749476 | 4:-68809563 | MsG0480022141.01.T01:CDS |
| GTGGTGACACGCTCACATCG+GGG | 0.751626 | 4:+68809435 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GGCATCGATGACTAATTTTT+CGG | + | Chr4:68809576-68809595 | None:intergenic | 35.0% |
| AAAGAGAAGTTGAGGAGCTT+AGG | - | Chr4:68809426-68809445 | MsG0480022141.01.T01:CDS | 40.0% | |
| AAGAGAAGTTGAGGAGCTTA+GGG | - | Chr4:68809427-68809446 | MsG0480022141.01.T01:CDS | 40.0% | |
| AATACCTGAAGAGGTGGTTT+GGG | - | Chr4:68809375-68809394 | MsG0480022141.01.T01:CDS | 40.0% | |
| CACATTGATAAGGTTGAAGC+TGG | + | Chr4:68809506-68809525 | None:intergenic | 40.0% | |
| GGTCTTTAACTGAGCAAAAC+CGG | - | Chr4:68809396-68809415 | MsG0480022141.01.T01:CDS | 40.0% | |
| ACATCGGGGACACATTGATA+AGG | + | Chr4:68809516-68809535 | None:intergenic | 45.0% | |
| CAACTTCTCTTTGTAGCCTC+CGG | + | Chr4:68809418-68809437 | None:intergenic | 45.0% | |
| CTGTGAGGAGATAACACAGT+AGG | + | Chr4:68809470-68809489 | None:intergenic | 45.0% | |
| CTTTAACTGAGCAAAACCGG+AGG | - | Chr4:68809399-68809418 | MsG0480022141.01.T01:CDS | 45.0% | |
| GAATACCTGAAGAGGTGGTT+TGG | - | Chr4:68809374-68809393 | MsG0480022141.01.T01:CDS | 45.0% | |
| GAGCTTAGGGCTATGAAGAT+GGG | - | Chr4:68809440-68809459 | MsG0480022141.01.T01:CDS | 45.0% | |
| GAGGCTACAAAGAGAAGTTG+AGG | - | Chr4:68809418-68809437 | MsG0480022141.01.T01:CDS | 45.0% | |
| GATAAGGTTGAAGCTGGAAG+AGG | + | Chr4:68809500-68809519 | None:intergenic | 45.0% | |
| TGAGGAGATAACACAGTAGG+TGG | + | Chr4:68809467-68809486 | None:intergenic | 45.0% | |
| ! | CACTCTAGGGGACAAAATGA+CGG | + | Chr4:68809597-68809616 | None:intergenic | 45.0% |
| !! | TAATTTTTCGGCAGCGACTG+TGG | + | Chr4:68809564-68809583 | None:intergenic | 45.0% |
| AAGACCCAAACCACCTCTTC+AGG | + | Chr4:68809382-68809401 | None:intergenic | 50.0% | |
| AGTGCGAATACCTGAAGAGG+TGG | - | Chr4:68809369-68809388 | MsG0480022141.01.T01:CDS | 50.0% | |
| GGAGCTTAGGGCTATGAAGA+TGG | - | Chr4:68809439-68809458 | MsG0480022141.01.T01:CDS | 50.0% | |
| TGGAGTGCGAATACCTGAAG+AGG | - | Chr4:68809366-68809385 | MsG0480022141.01.T01:CDS | 50.0% | |
| GGAAGAGGCTCAGAACTGTG+AGG | + | Chr4:68809485-68809504 | None:intergenic | 55.0% | |
| ! | TGGTGGTGACACGCTCACAT+CGG | + | Chr4:68809532-68809551 | None:intergenic | 55.0% |
| !! | TTTTTCGGCAGCGACTGTGG+CGG | + | Chr4:68809561-68809580 | None:intergenic | 55.0% |
| AGCGACTGTGGCGGTGGTAT+TGG | + | Chr4:68809552-68809571 | None:intergenic | 60.0% | |
| GGTGGTGACACGCTCACATC+GGG | + | Chr4:68809531-68809550 | None:intergenic | 60.0% | |
| GTGGTGACACGCTCACATCG+GGG | + | Chr4:68809530-68809549 | None:intergenic | 60.0% | |
| ! | GACTGTGGCGGTGGTATTGG+TGG | + | Chr4:68809549-68809568 | None:intergenic | 60.0% |
| TTCGGCAGCGACTGTGGCGG+TGG | + | Chr4:68809558-68809577 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 68809365 | 68809619 | 68809365 | ID=MsG0480022141.01;Name=MsG0480022141.01 |
| Chr4 | mRNA | 68809365 | 68809619 | 68809365 | ID=MsG0480022141.01.T01;Parent=MsG0480022141.01;Name=MsG0480022141.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|84 |
| Chr4 | exon | 68809365 | 68809619 | 68809365 | ID=MsG0480022141.01.T01:exon:18912;Parent=MsG0480022141.01.T01 |
| Chr4 | CDS | 68809365 | 68809619 | 68809365 | ID=MsG0480022141.01.T01:cds;Parent=MsG0480022141.01.T01 |
| Gene Sequence |
| Protein sequence |
