AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0032880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032880 MtrunA17_Chr1g0192201 98.810 168 2 0 1 168 1 168 3.26e-121 338
Msa0032880 MtrunA17_Chr1g0192171 98.214 168 3 0 1 168 1 168 2.27e-120 336
Msa0032880 MtrunA17_Chr2g0295871 86.310 168 22 1 1 168 1 167 1.09e-103 294
Msa0032880 MtrunA17_Chr6g0479151 81.988 161 28 1 1 161 1 160 3.18e-91 262
Msa0032880 MtrunA17_Chr1g0192161 86.047 129 18 0 19 147 9 137 1.61e-65 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032880 AT3G16640.1 78.571 168 36 0 1 168 1 168 8.01e-97 277
Msa0032880 AT3G05540.1 79.882 169 32 2 1 168 1 168 3.29e-90 260

Find 49 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATAGTACTCTGGTAATTT+TGG 0.116227 1_1:+60031697 Msa0032880:three_prime_UTR
TTGACATTGTTGACACTTTC+AGG 0.139441 1_1:+60031234 Msa0032880:CDS
ATTATAAAATTTCAGTAGTA+TGG 0.248085 1_1:+60031820 Msa0032880:intron
GAGTTTACTATTCTCTAATT+AGG 0.257485 1_1:+60031745 Msa0032880:three_prime_UTR
AATTGTAGGTCTTTGAGTTT+TGG 0.259902 1_1:-60031398 None:intergenic
GTTGCTGACATCTAAATTAG+AGG 0.319278 1_1:+60031319 Msa0032880:CDS
TGTTGACACTTTCAGGCTTC+AGG 0.327729 1_1:+60031241 Msa0032880:CDS
ACACATTTAGTTATCATGTT+AGG 0.332046 1_1:+60031648 Msa0032880:three_prime_UTR
AAATTTCAGTAGTATGGTTA+TGG 0.340189 1_1:+60031826 Msa0032880:intron
CGCAACCATGCTTGTTTACC+AGG 0.349778 1_1:+60030159 Msa0032880:exon
GATGAAACACATACAATTGT+AGG 0.370851 1_1:-60031412 None:intergenic
CTACTTTGCCTATGGCTTGA+AGG 0.378916 1_1:+60031611 Msa0032880:CDS
GTTTACCAGGATCTTCTTAC+GGG 0.390880 1_1:+60030172 Msa0032880:CDS
TGTTTACCAGGATCTTCTTA+CGG 0.395300 1_1:+60030171 Msa0032880:CDS
CCATTCTGAATTTCCTTGTA+GGG 0.421805 1_1:-60030963 None:intergenic
TCCATTCTGAATTTCCTTGT+AGG 0.427172 1_1:-60030964 None:intergenic
AATTGCTTCTTATCAAAAGC+TGG 0.428621 1_1:-60031269 None:intergenic
GAATGGAATGTTGTGGGAAG+TGG 0.443613 1_1:+60030980 Msa0032880:CDS
CCTTGTGTTTGCTTACTACA+AGG 0.455642 1_1:+60031563 Msa0032880:CDS
TAGGCAAAGTAGAGAAAAGT+TGG 0.474181 1_1:-60031600 None:intergenic
TATTTGCAGTGGGTTGTTCA+AGG 0.483236 1_1:+60031125 Msa0032880:intron
CTCTGACTCATTCCCCTACA+AGG 0.486361 1_1:+60030950 Msa0032880:CDS
GGAGCAGTGGATGTGAATAT+TGG 0.501116 1_1:+60031146 Msa0032880:CDS
AATAAGAGAGAAGCAAAAGA+AGG 0.503108 1_1:-60030124 None:intergenic
GGTGCTAACCCTTCTGCTGA+GGG 0.509671 1_1:+60031167 Msa0032880:CDS
CTTGATTTCCTTCAAGCCAT+AGG 0.517168 1_1:-60031619 None:intergenic
TTTACTATTCTCTAATTAGG+AGG 0.521787 1_1:+60031748 Msa0032880:three_prime_UTR
ATTCTTAATCGATAGTACTC+TGG 0.532748 1_1:+60031688 Msa0032880:three_prime_UTR
AATTCAGAATGGAATGTTGT+GGG 0.534489 1_1:+60030974 Msa0032880:CDS
TCGTCACCACCCTCAGCAGA+AGG 0.538139 1_1:-60031176 None:intergenic
GTGTTTGCTTACTACAAGGA+TGG 0.538874 1_1:+60031567 Msa0032880:CDS
CCCTACAAGGAAATTCAGAA+TGG 0.541188 1_1:+60030963 Msa0032880:CDS
CATTCTGAATTTCCTTGTAG+GGG 0.549656 1_1:-60030962 None:intergenic
TGGTGCTAACCCTTCTGCTG+AGG 0.553542 1_1:+60031166 Msa0032880:CDS
GTTTACCCGTAAGAAGATCC+TGG 0.562606 1_1:-60030177 None:intergenic
GGGGAGAGCATGCATGATGA+TGG 0.565967 1_1:+60031537 Msa0032880:CDS
AAATTCAGAATGGAATGTTG+TGG 0.574500 1_1:+60030973 Msa0032880:CDS
GTTGTGGGAAGTGGAAGGAA+AGG 0.585239 1_1:+60030989 Msa0032880:CDS
TTCTGCTGAGGGTGGTGACG+AGG 0.592413 1_1:+60031178 Msa0032880:CDS
CTGGTAAACAAGCATGGTTG+CGG 0.598178 1_1:-60030158 None:intergenic
AAGATCCTGGTAAACAAGCA+TGG 0.602746 1_1:-60030164 None:intergenic
GTAGAGAAAAGTTGGATCAG+TGG 0.605758 1_1:-60031592 None:intergenic
GCTAACCCTTCTGCTGAGGG+TGG 0.615863 1_1:+60031170 Msa0032880:CDS
CGTCACCACCCTCAGCAGAA+GGG 0.628440 1_1:-60031175 None:intergenic
CCTTGTAGTAAGCAAACACA+AGG 0.635355 1_1:-60031563 None:intergenic
GTGGGTTGTTCAAGGAGCAG+TGG 0.636439 1_1:+60031133 Msa0032880:intron
GAGGGTGGTGACGAGGATGA+CGG 0.661706 1_1:+60031185 Msa0032880:CDS
GGAATGTTGTGGGAAGTGGA+AGG 0.687071 1_1:+60030984 Msa0032880:CDS
ATATTGTTCTCAGAGCAACA+TGG 0.711223 1_1:+60031793 Msa0032880:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTAATAGAATTTGT+TGG + chr1_1:60030657-60030676 Msa0032880:intron 10.0%
!! TTTATTTATGATCATATATT+TGG - chr1_1:60031076-60031095 None:intergenic 10.0%
!!! TTATATATTAACCTTTTGTA+AGG + chr1_1:60030443-60030462 Msa0032880:intron 15.0%
!!! TTTTTTTTTTTTTTGACACA+AGG + chr1_1:60030495-60030514 Msa0032880:intron 15.0%
!! AAAACAAAAATTCATTCAAA+TGG + chr1_1:60030874-60030893 Msa0032880:intron 15.0%
!! ATTATAAAATTTCAGTAGTA+TGG + chr1_1:60031820-60031839 Msa0032880:intron 15.0%
!! AAAGTAAAAGAAACCTAATT+GGG - chr1_1:60030237-60030256 None:intergenic 20.0%
!! AAAAGTAAAAGAAACCTAAT+TGG - chr1_1:60030238-60030257 None:intergenic 20.0%
!! TTATCATTCATCGTAATATT+TGG - chr1_1:60030316-60030335 None:intergenic 20.0%
!!! TTTTTCATAAACTTCACTTT+TGG + chr1_1:60030391-60030410 Msa0032880:intron 20.0%
!!! ACAAGGTTAAAATACTTTTT+TGG + chr1_1:60030512-60030531 Msa0032880:intron 20.0%
!! GATAATAATGAAAAACCTAA+AGG - chr1_1:60030631-60030650 None:intergenic 20.0%
!! TAATCTTAAATATTAGTAGC+AGG - chr1_1:60030824-60030843 None:intergenic 20.0%
!!! ATTATCTTTCATCTCTTTTT+TGG + chr1_1:60031435-60031454 Msa0032880:intron 20.0%
!!! ATTAAGTTTTCTCTTTTTTG+TGG + chr1_1:60031462-60031481 Msa0032880:intron 20.0%
!!! TTTTCTCCATTGTTTTTCAT+AGG + chr1_1:60030254-60030273 Msa0032880:intron 25.0%
! CAAAATCCTATGAAAAACAA+TGG - chr1_1:60030263-60030282 None:intergenic 25.0%
! AAATTCATTCACTAAACACA+GGG - chr1_1:60030348-60030367 None:intergenic 25.0%
! AATCTTAACAACCTTACAAA+AGG - chr1_1:60030457-60030476 None:intergenic 25.0%
! TTGGAAAACCTTAAATTTCT+TGG + chr1_1:60030531-60030550 Msa0032880:intron 25.0%
! TGGAAAACCTTAAATTTCTT+GGG + chr1_1:60030532-60030551 Msa0032880:intron 25.0%
! ACTATTATAGATCAAACTTG+TGG + chr1_1:60030566-60030585 Msa0032880:intron 25.0%
! AAATATTACTTAGCAGTTTG+TGG - chr1_1:60030735-60030754 None:intergenic 25.0%
! AAAATCCACAATCAAGTATT+AGG - chr1_1:60030784-60030803 None:intergenic 25.0%
! ACACATTTAGTTATCATGTT+AGG + chr1_1:60031648-60031667 Msa0032880:three_prime_UTR 25.0%
! GAGTTTACTATTCTCTAATT+AGG + chr1_1:60031745-60031764 Msa0032880:three_prime_UTR 25.0%
! TTTACTATTCTCTAATTAGG+AGG + chr1_1:60031748-60031767 Msa0032880:three_prime_UTR 25.0%
! AAATTTCAGTAGTATGGTTA+TGG + chr1_1:60031826-60031845 Msa0032880:intron 25.0%
AATAAGAGAGAAGCAAAAGA+AGG - chr1_1:60030127-60030146 None:intergenic 30.0%
ACAAAAAACTCAAAGATGCA+AGG - chr1_1:60030202-60030221 None:intergenic 30.0%
! TTTTACTTTACCACCCAATT+AGG + chr1_1:60030221-60030240 Msa0032880:intron 30.0%
GAAATTCATTCACTAAACAC+AGG - chr1_1:60030349-60030368 None:intergenic 30.0%
GATCTATAATAGTGTGTGTT+TGG - chr1_1:60030559-60030578 None:intergenic 30.0%
!! AGTTTTATGTTTGTGTTCTG+TGG + chr1_1:60030600-60030619 Msa0032880:intron 30.0%
! CTTTGTTGATGAATTTGTCT+AGG + chr1_1:60030915-60030934 Msa0032880:intron 30.0%
AAATTCAGAATGGAATGTTG+TGG + chr1_1:60030973-60030992 Msa0032880:CDS 30.0%
AATTCAGAATGGAATGTTGT+GGG + chr1_1:60030974-60030993 Msa0032880:CDS 30.0%
CACATCACACAATAAACTTT+AGG - chr1_1:60031103-60031122 None:intergenic 30.0%
AATTGCTTCTTATCAAAAGC+TGG - chr1_1:60031272-60031291 None:intergenic 30.0%
!! ATTGTAGGTCTTTGAGTTTT+GGG - chr1_1:60031400-60031419 None:intergenic 30.0%
!! AATTGTAGGTCTTTGAGTTT+TGG - chr1_1:60031401-60031420 None:intergenic 30.0%
GATGAAACACATACAATTGT+AGG - chr1_1:60031415-60031434 None:intergenic 30.0%
!!! ATCAATGTTGCAGTTTTGTT+GGG + chr1_1:60031517-60031536 Msa0032880:intron 30.0%
ATTCTTAATCGATAGTACTC+TGG + chr1_1:60031688-60031707 Msa0032880:three_prime_UTR 30.0%
TGTTTACCAGGATCTTCTTA+CGG + chr1_1:60030171-60030190 Msa0032880:CDS 35.0%
GTAAAAGAAACCTAATTGGG+TGG - chr1_1:60030234-60030253 None:intergenic 35.0%
GTTTGGTCCCAAGAAATTTA+AGG - chr1_1:60030542-60030561 None:intergenic 35.0%
TGTTCTGTGGTTTAACCTTT+AGG + chr1_1:60030613-60030632 Msa0032880:intron 35.0%
GTTAGGCTAATCGTGTTTAA+GGG - chr1_1:60030762-60030781 None:intergenic 35.0%
CCACAATCAAGTATTAGGTT+AGG - chr1_1:60030779-60030798 None:intergenic 35.0%
CCTAACCTAATACTTGATTG+TGG + chr1_1:60030776-60030795 Msa0032880:intron 35.0%
CATTCTGAATTTCCTTGTAG+GGG - chr1_1:60030965-60030984 None:intergenic 35.0%
CCATTCTGAATTTCCTTGTA+GGG - chr1_1:60030966-60030985 None:intergenic 35.0%
TCCATTCTGAATTTCCTTGT+AGG - chr1_1:60030967-60030986 None:intergenic 35.0%
TGTGATGTGATTATTTGCAG+TGG + chr1_1:60031114-60031133 Msa0032880:intron 35.0%
GTGATGTGATTATTTGCAGT+GGG + chr1_1:60031115-60031134 Msa0032880:intron 35.0%
TTGACATTGTTGACACTTTC+AGG + chr1_1:60031234-60031253 Msa0032880:CDS 35.0%
GTTGCTGACATCTAAATTAG+AGG + chr1_1:60031319-60031338 Msa0032880:CDS 35.0%
!! CATCAATGTTGCAGTTTTGT+TGG + chr1_1:60031516-60031535 Msa0032880:intron 35.0%
!!! TCAATGTTGCAGTTTTGTTG+GGG + chr1_1:60031518-60031537 Msa0032880:intron 35.0%
TAGGCAAAGTAGAGAAAAGT+TGG - chr1_1:60031603-60031622 None:intergenic 35.0%
! ACTTTTCTCTACTTTGCCTA+TGG + chr1_1:60031603-60031622 Msa0032880:CDS 35.0%
! CGATAGTACTCTGGTAATTT+TGG + chr1_1:60031697-60031716 Msa0032880:three_prime_UTR 35.0%
ATATTGTTCTCAGAGCAACA+TGG + chr1_1:60031793-60031812 Msa0032880:three_prime_UTR 35.0%
AAGATCCTGGTAAACAAGCA+TGG - chr1_1:60030167-60030186 None:intergenic 40.0%
GTTTACCAGGATCTTCTTAC+GGG + chr1_1:60030172-60030191 Msa0032880:CDS 40.0%
AGTTGTTGTGTTTGTGACAG+AGG + chr1_1:60030691-60030710 Msa0032880:intron 40.0%
GGTTAGGCTAATCGTGTTTA+AGG - chr1_1:60030763-60030782 None:intergenic 40.0%
CCCTACAAGGAAATTCAGAA+TGG + chr1_1:60030963-60030982 Msa0032880:CDS 40.0%
!! TATTTGCAGTGGGTTGTTCA+AGG + chr1_1:60031125-60031144 Msa0032880:intron 40.0%
CCTTGTAGTAAGCAAACACA+AGG - chr1_1:60031566-60031585 None:intergenic 40.0%
CCTTGTGTTTGCTTACTACA+AGG + chr1_1:60031563-60031582 Msa0032880:CDS 40.0%
GTGTTTGCTTACTACAAGGA+TGG + chr1_1:60031567-60031586 Msa0032880:CDS 40.0%
GTAGAGAAAAGTTGGATCAG+TGG - chr1_1:60031595-60031614 None:intergenic 40.0%
CTTGATTTCCTTCAAGCCAT+AGG - chr1_1:60031622-60031641 None:intergenic 40.0%
CTGGTAAACAAGCATGGTTG+CGG - chr1_1:60030161-60030180 None:intergenic 45.0%
GTTTACCCGTAAGAAGATCC+TGG - chr1_1:60030180-60030199 None:intergenic 45.0%
GAATGGAATGTTGTGGGAAG+TGG + chr1_1:60030980-60030999 Msa0032880:CDS 45.0%
GGAGCAGTGGATGTGAATAT+TGG + chr1_1:60031146-60031165 Msa0032880:CDS 45.0%
TGTTGACACTTTCAGGCTTC+AGG + chr1_1:60031241-60031260 Msa0032880:CDS 45.0%
!!! GGTCTTTGAGTTTTGGGAGA+AGG - chr1_1:60031394-60031413 None:intergenic 45.0%
CTACTTTGCCTATGGCTTGA+AGG + chr1_1:60031611-60031630 Msa0032880:CDS 45.0%
!!! ATTCTATTAAATTAAAATTT+TGG - chr1_1:60030653-60030672 None:intergenic 5.0%
CGCAACCATGCTTGTTTACC+AGG + chr1_1:60030159-60030178 Msa0032880:exon 50.0%
CTCTGACTCATTCCCCTACA+AGG + chr1_1:60030950-60030969 Msa0032880:CDS 50.0%
GGAATGTTGTGGGAAGTGGA+AGG + chr1_1:60030984-60031003 Msa0032880:CDS 50.0%
GTTGTGGGAAGTGGAAGGAA+AGG + chr1_1:60030989-60031008 Msa0032880:CDS 50.0%
GGGAAGTGGAAGGAAAGGTT+AGG + chr1_1:60030994-60031013 Msa0032880:intron 50.0%
! GTGGGTTGTTCAAGGAGCAG+TGG + chr1_1:60031133-60031152 Msa0032880:intron 55.0%
! TGGTGCTAACCCTTCTGCTG+AGG + chr1_1:60031166-60031185 Msa0032880:CDS 55.0%
GGTGCTAACCCTTCTGCTGA+GGG + chr1_1:60031167-60031186 Msa0032880:CDS 55.0%
! GGGGAGAGCATGCATGATGA+TGG + chr1_1:60031537-60031556 Msa0032880:CDS 55.0%
GCTAACCCTTCTGCTGAGGG+TGG + chr1_1:60031170-60031189 Msa0032880:CDS 60.0%
CGTCACCACCCTCAGCAGAA+GGG - chr1_1:60031178-60031197 None:intergenic 60.0%
TCGTCACCACCCTCAGCAGA+AGG - chr1_1:60031179-60031198 None:intergenic 60.0%
! TTCTGCTGAGGGTGGTGACG+AGG + chr1_1:60031178-60031197 Msa0032880:CDS 60.0%
! GAGGGTGGTGACGAGGATGA+CGG + chr1_1:60031185-60031204 Msa0032880:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 60030099 60031878 60030099 ID=Msa0032880;Name=Msa0032880
chr1_1 mRNA 60030099 60031878 60030099 ID=Msa0032880-mRNA-1;Parent=Msa0032880;Name=Msa0032880-mRNA-1;_AED=0.13;_eAED=0.13;_QI=67|1|1|1|0.75|0.6|5|211|168
chr1_1 exon 60030099 60030193 60030099 ID=Msa0032880-mRNA-1:exon:18705;Parent=Msa0032880-mRNA-1
chr1_1 exon 60030937 60031010 60030937 ID=Msa0032880-mRNA-1:exon:18706;Parent=Msa0032880-mRNA-1
chr1_1 exon 60031134 60031420 60031134 ID=Msa0032880-mRNA-1:exon:18707;Parent=Msa0032880-mRNA-1
chr1_1 exon 60031530 60031814 60031530 ID=Msa0032880-mRNA-1:exon:18708;Parent=Msa0032880-mRNA-1
chr1_1 exon 60031835 60031878 60031835 ID=Msa0032880-mRNA-1:exon:18709;Parent=Msa0032880-mRNA-1
chr1_1 five_prime_UTR 60030099 60030165 60030099 ID=Msa0032880-mRNA-1:five_prime_utr;Parent=Msa0032880-mRNA-1
chr1_1 CDS 60030166 60030193 60030166 ID=Msa0032880-mRNA-1:cds;Parent=Msa0032880-mRNA-1
chr1_1 CDS 60030937 60031010 60030937 ID=Msa0032880-mRNA-1:cds;Parent=Msa0032880-mRNA-1
chr1_1 CDS 60031134 60031420 60031134 ID=Msa0032880-mRNA-1:cds;Parent=Msa0032880-mRNA-1
chr1_1 CDS 60031530 60031647 60031530 ID=Msa0032880-mRNA-1:cds;Parent=Msa0032880-mRNA-1
chr1_1 three_prime_UTR 60031648 60031814 60031648 ID=Msa0032880-mRNA-1:three_prime_utr;Parent=Msa0032880-mRNA-1
chr1_1 three_prime_UTR 60031835 60031878 60031835 ID=Msa0032880-mRNA-1:three_prime_utr;Parent=Msa0032880-mRNA-1
Gene Sequence

>Msa0032880

ATGCTTGTTTACCAGGATCTTCTTACGGGTGATGAGCTTCTCTCTGACTCATTCCCCTACAAGGAAATTCAGAATGGAATGTTGTGGGAAGTGGAAGGAAAGTGGGTTGTTCAAGGAGCAGTGGATGTGAATATTGGTGCTAACCCTTCTGCTGAGGGTGGTGACGAGGATGACGGTGTTGATGATCAAGCTGTCAAAGTTGTTGACATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGAAGCAATTTGTTACTTATATGAAGAGATATATTAAGTTGCTGACATCTAAATTAGAGGCAGAGAAGCAAGAGTTGTTCAAGAAGCACATTGAAGCAGCAACTAAGTTCCTTCTCCCAAAACTCAAAGACCTACAATTTTTTGTTGGGGAGAGCATGCATGATGATGGAAGCCTTGTGTTTGCTTACTACAAGGATGGTGCCACTGATCCAACTTTTCTCTACTTTGCCTATGGCTTGAAGGAAATCAAGTGTTAA

Protein sequence

>Msa0032880

MLVYQDLLTGDELLSDSFPYKEIQNGMLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVVDIVDTFRLQEQPAFDKKQFVTYMKRYIKLLTSKLEAEKQELFKKHIEAATKFLLPKLKDLQFFVGESMHDDGSLVFAYYKDGATDPTFLYFAYGLKEIKC*