AlfalfaGEDB | Msa0032860

M. sativa cultivar zhongmu-4 / Msa0032860

KEGG: -
GO: -
PFAM: PF00838.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032860	sp\|Q5J907\|TCTP_ELAGV	90.141	142	14	0	1	142	27	168	6.34e-91	263

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004840	Msa0032860	0.807427	5.081607e-50	-8.615850e-47
Msa0017320	Msa0032860	0.804547	2.050148e-49	-8.615850e-47
Msa0029850	Msa0032860	0.801847	7.422174e-49	-8.615850e-47
Msa0032860	Msa0032880	0.899397	2.063341e-77	-8.615850e-47
Msa0032860	Msa0037880	0.813047	3.118564e-51	-8.615850e-47
Msa0032860	Msa0038870	0.823293	1.497883e-53	-8.615850e-47
Msa0032860	Msa0065950	0.822204	2.684978e-53	-8.615850e-47
Msa0032860	Msa0084430	0.804079	2.566223e-49	-8.615850e-47
Msa0032860	Msa0086420	0.833778	4.386004e-56	-8.615850e-47
Msa0032860	Msa0089820	0.806797	6.909977e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032860	MtrunA17_Chr1g0192171	99.296	142	1	0	1	142	27	168	7.57e-102	288
Msa0032860	MtrunA17_Chr1g0192201	98.592	142	2	0	1	142	27	168	1.07e-100	285
Msa0032860	MtrunA17_Chr2g0295871	84.507	142	21	1	1	142	27	167	8.64e-84	243
Msa0032860	MtrunA17_Chr1g0192161	97.345	113	3	0	9	121	25	137	1.17e-75	221
Msa0032860	MtrunA17_Chr6g0479151	80.741	135	25	1	1	135	27	160	2.40e-73	216

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032860	AT3G16640.1	75.352	142	35	0	1	142	27	168	4.38e-77	226
Msa0032860	AT3G05540.1	77.622	143	30	2	1	142	27	168	1.65e-70	209

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Find 32 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTGAGAACGGAATGCTT+TGG	0.446609	1_1:+60017797	Msa0032860:exon
AATTGAGAACGGAATGCTTT+GGG	0.399647	1_1:+60017798	Msa0032860:exon
AATTGCTTCTTATCAAAAGC+TGG	0.428621	1_1:-60018092	None:intergenic
AATTGTAGGTCTTTGAGTTT+TGG	0.259902	1_1:-60018221	None:intergenic
CCCTACAAGGAAATTGAGAA+CGG	0.603248	1_1:+60017787	Msa0032860:five_prime_UTR
CCGTTCTCAATTTCCTTGTA+GGG	0.410758	1_1:-60017787	None:intergenic
CGTTCTCAATTTCCTTGTAG+GGG	0.538126	1_1:-60017786	None:intergenic
CTACTTTGCTTATGGCTTGA+AGG	0.363173	1_1:+60018434	Msa0032860:CDS
CTCTGACTCATTCCCCTACA+AGG	0.486361	1_1:+60017774	Msa0032860:five_prime_UTR
CTTTGTAGTAAGCAAACACA+AGG	0.596130	1_1:-60018386	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTGACATAAAAACTAAAGCA+TGG	-	chr1_1:60017881-60017900	None:intergenic	30.0%
CACATTACACAGTATACTTT+AGG	-	chr1_1:60017926-60017945	None:intergenic	30.0%
AATTGCTTCTTATCAAAAGC+TGG	-	chr1_1:60018095-60018114	None:intergenic	30.0%
TAAGCAAAGTAGAGAAAAGT+TGG	-	chr1_1:60018426-60018445	None:intergenic	30.0%
CTGACATAAAAACTAAAGCA+TGG	-	chr1_1:60017881-60017900	None:intergenic	30.0%
CACATTACACAGTATACTTT+AGG	-	chr1_1:60017926-60017945	None:intergenic	30.0%
AATTGCTTCTTATCAAAAGC+TGG	-	chr1_1:60018095-60018114	None:intergenic	30.0%
TAAGCAAAGTAGAGAAAAGT+TGG	-	chr1_1:60018426-60018445	None:intergenic	30.0%
AAATTGAGAACGGAATGCTT+TGG	+	chr1_1:60017797-60017816	Msa0032860:exon	35.0%
AATTGAGAACGGAATGCTTT+GGG	+	chr1_1:60017798-60017817	Msa0032860:exon	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	60017715	60018623	60017715	ID=Msa0032860;Name=Msa0032860
chr1_1	mRNA	60017715	60018623	60017715	ID=Msa0032860-mRNA-1;Parent=Msa0032860;Name=Msa0032860-mRNA-1;_AED=0.06;_eAED=0.06;_QI=96\|1\|1\|1\|1\|1\|3\|153\|142
chr1_1	exon	60017715	60017834	60017715	ID=Msa0032860-mRNA-1:exon:18689;Parent=Msa0032860-mRNA-1
chr1_1	exon	60017957	60018243	60017957	ID=Msa0032860-mRNA-1:exon:18690;Parent=Msa0032860-mRNA-1
chr1_1	exon	60018353	60018623	60018353	ID=Msa0032860-mRNA-1:exon:18691;Parent=Msa0032860-mRNA-1
chr1_1	five_prime_UTR	60017715	60017810	60017715	ID=Msa0032860-mRNA-1:five_prime_utr;Parent=Msa0032860-mRNA-1
chr1_1	CDS	60017811	60017834	60017811	ID=Msa0032860-mRNA-1:cds;Parent=Msa0032860-mRNA-1
chr1_1	CDS	60017957	60018243	60017957	ID=Msa0032860-mRNA-1:cds;Parent=Msa0032860-mRNA-1
chr1_1	CDS	60018353	60018470	60018353	ID=Msa0032860-mRNA-1:cds;Parent=Msa0032860-mRNA-1
chr1_1	three_prime_UTR	60018471	60018623	60018471	ID=Msa0032860-mRNA-1:three_prime_utr;Parent=Msa0032860-mRNA-1

Gene Sequence

>Msa0032860

ATGCTTTGGGAAGTGGAAGGAAAGTGGGTTGTTCAAGGAGCAGTGGATGTGAACATTGGTGCTAATCCTTCTGCTGAGGGCGGAGATGAGGACGACGGTGTTGATGATCAAGCTGTGAAAGTGGTTGACATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGAAGCAATTTGTTACTTATATGAAGAGATATATTAAGTTGCTGACATCTAAATTAGAGCCAGAAAAGCAAGAGTTGTTCAAGAAGCACATTGAAGCAGCAACTAAGTTTCTCCTCCCAAAACTCAAAGACCTACAATTTTTTGTTGGGGAGAGCATGCATGATGATGGAAGCCTTGTGTTTGCTTACTACAAAGATGGTGCCACTGATCCAACTTTTCTCTACTTTGCTTATGGCTTGAAGGAAATCAAGTGTTAA

Protein sequence

>Msa0032860

MLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVVDIVDTFRLQEQPAFDKKQFVTYMKRYIKLLTSKLEPEKQELFKKHIEAATKFLLPKLKDLQFFVGESMHDDGSLVFAYYKDGATDPTFLYFAYGLKEIKC*