AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0032860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032860 MtrunA17_Chr1g0192171 99.296 142 1 0 1 142 27 168 7.57e-102 288
Msa0032860 MtrunA17_Chr1g0192201 98.592 142 2 0 1 142 27 168 1.07e-100 285
Msa0032860 MtrunA17_Chr2g0295871 84.507 142 21 1 1 142 27 167 8.64e-84 243
Msa0032860 MtrunA17_Chr1g0192161 97.345 113 3 0 9 121 25 137 1.17e-75 221
Msa0032860 MtrunA17_Chr6g0479151 80.741 135 25 1 1 135 27 160 2.40e-73 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032860 AT3G16640.1 75.352 142 35 0 1 142 27 168 4.38e-77 226
Msa0032860 AT3G05540.1 77.622 143 30 2 1 142 27 168 1.65e-70 209

Find 32 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACATTGTTGACACTTTC+AGG 0.150814 1_1:+60018057 Msa0032860:CDS
AATTGTAGGTCTTTGAGTTT+TGG 0.259902 1_1:-60018221 None:intergenic
TGTTTAAATTGATGTTTAAT+TGG 0.308821 1_1:+60018495 Msa0032860:three_prime_UTR
TGTTGACACTTTCAGGCTTC+AGG 0.327729 1_1:+60018064 Msa0032860:CDS
CTACTTTGCTTATGGCTTGA+AGG 0.363173 1_1:+60018434 Msa0032860:CDS
TCCGTTCTCAATTTCCTTGT+AGG 0.371448 1_1:-60017788 None:intergenic
GATGAAATACATACAATTGT+AGG 0.374980 1_1:-60018235 None:intergenic
AATTGAGAACGGAATGCTTT+GGG 0.399647 1_1:+60017798 Msa0032860:exon
CCGTTCTCAATTTCCTTGTA+GGG 0.410758 1_1:-60017787 None:intergenic
GAACGGAATGCTTTGGGAAG+TGG 0.428530 1_1:+60017804 Msa0032860:exon
AATTGCTTCTTATCAAAAGC+TGG 0.428621 1_1:-60018092 None:intergenic
AAATTGAGAACGGAATGCTT+TGG 0.446609 1_1:+60017797 Msa0032860:exon
TAAGCAAAGTAGAGAAAAGT+TGG 0.453692 1_1:-60018423 None:intergenic
GTGTTTGCTTACTACAAAGA+TGG 0.481773 1_1:+60018390 Msa0032860:CDS
CTCTGACTCATTCCCCTACA+AGG 0.486361 1_1:+60017774 Msa0032860:five_prime_UTR
CTTTGTTGATGAATTTGTCT+AGG 0.516142 1_1:+60017739 Msa0032860:five_prime_UTR
GCTTTGGGAAGTGGAAGGAA+AGG 0.531642 1_1:+60017813 Msa0032860:CDS
TCATCTCCGCCCTCAGCAGA+AGG 0.531916 1_1:-60017999 None:intergenic
TGGTGCTAATCCTTCTGCTG+AGG 0.536435 1_1:+60017989 Msa0032860:CDS
CGTTCTCAATTTCCTTGTAG+GGG 0.538126 1_1:-60017786 None:intergenic
TTCTGCTGAGGGCGGAGATG+AGG 0.539950 1_1:+60018001 Msa0032860:CDS
GGTGCTAATCCTTCTGCTGA+GGG 0.547301 1_1:+60017990 Msa0032860:CDS
GGGGAGAGCATGCATGATGA+TGG 0.565967 1_1:+60018360 Msa0032860:CDS
CTTTGTAGTAAGCAAACACA+AGG 0.596130 1_1:-60018386 None:intergenic
CCCTACAAGGAAATTGAGAA+CGG 0.603248 1_1:+60017787 Msa0032860:five_prime_UTR
GTAGAGAAAAGTTGGATCAG+TGG 0.605758 1_1:-60018415 None:intergenic
TGATGATCAAGCTGTGAAAG+TGG 0.621314 1_1:+60018034 Msa0032860:CDS
GCTAATCCTTCTGCTGAGGG+CGG 0.631354 1_1:+60017993 Msa0032860:CDS
GTGGGTTGTTCAAGGAGCAG+TGG 0.636439 1_1:+60017956 Msa0032860:intron
GGAGCAGTGGATGTGAACAT+TGG 0.696184 1_1:+60017969 Msa0032860:CDS
GGAATGCTTTGGGAAGTGGA+AGG 0.706482 1_1:+60017808 Msa0032860:exon
GAGGGCGGAGATGAGGACGA+CGG 0.740386 1_1:+60018008 Msa0032860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTACATTTTATTTTTATA+TGG + chr1_1:60018584-60018603 Msa0032860:three_prime_UTR 10.0%
!!! GATTACATTTTATTTTTATA+TGG + chr1_1:60018584-60018603 Msa0032860:three_prime_UTR 10.0%
!!! ATTATTTTTCATCTCTTTTT+TGG + chr1_1:60018258-60018277 Msa0032860:intron 15.0%
!! TGTTTAAATTGATGTTTAAT+TGG + chr1_1:60018495-60018514 Msa0032860:three_prime_UTR 15.0%
!!! TGAATTTTTAAGTTCTAATT+AGG + chr1_1:60018548-60018567 Msa0032860:three_prime_UTR 15.0%
!!! ATTATTTTTCATCTCTTTTT+TGG + chr1_1:60018258-60018277 Msa0032860:intron 15.0%
!! TGTTTAAATTGATGTTTAAT+TGG + chr1_1:60018495-60018514 Msa0032860:three_prime_UTR 15.0%
!!! TGAATTTTTAAGTTCTAATT+AGG + chr1_1:60018548-60018567 Msa0032860:three_prime_UTR 15.0%
!!! ATTAAGTTTTCTCTTTTTTG+TGG + chr1_1:60018285-60018304 Msa0032860:intron 20.0%
!!! ATTTTTAAGTTCTAATTAGG+AGG + chr1_1:60018551-60018570 Msa0032860:three_prime_UTR 20.0%
!!! ATTAAGTTTTCTCTTTTTTG+TGG + chr1_1:60018285-60018304 Msa0032860:intron 20.0%
!!! ATTTTTAAGTTCTAATTAGG+AGG + chr1_1:60018551-60018570 Msa0032860:three_prime_UTR 20.0%
! GATGAAATACATACAATTGT+AGG - chr1_1:60018238-60018257 None:intergenic 25.0%
! GATGAAATACATACAATTGT+AGG - chr1_1:60018238-60018257 None:intergenic 25.0%
! CTTTGTTGATGAATTTGTCT+AGG + chr1_1:60017739-60017758 Msa0032860:five_prime_UTR 30.0%
CTGACATAAAAACTAAAGCA+TGG - chr1_1:60017881-60017900 None:intergenic 30.0%
CACATTACACAGTATACTTT+AGG - chr1_1:60017926-60017945 None:intergenic 30.0%
!!! TGTAATGTGATTTTTTGCAG+TGG + chr1_1:60017937-60017956 Msa0032860:intron 30.0%
!!! GTAATGTGATTTTTTGCAGT+GGG + chr1_1:60017938-60017957 Msa0032860:intron 30.0%
AATTGCTTCTTATCAAAAGC+TGG - chr1_1:60018095-60018114 None:intergenic 30.0%
!! ATTGTAGGTCTTTGAGTTTT+GGG - chr1_1:60018223-60018242 None:intergenic 30.0%
!! AATTGTAGGTCTTTGAGTTT+TGG - chr1_1:60018224-60018243 None:intergenic 30.0%
!!! ATCAATGTTGCAGTTTTGTT+GGG + chr1_1:60018340-60018359 Msa0032860:intron 30.0%
TAAGCAAAGTAGAGAAAAGT+TGG - chr1_1:60018426-60018445 None:intergenic 30.0%
! ACTTTTCTCTACTTTGCTTA+TGG + chr1_1:60018426-60018445 Msa0032860:CDS 30.0%
! CTTTGTTGATGAATTTGTCT+AGG + chr1_1:60017739-60017758 Msa0032860:five_prime_UTR 30.0%
CTGACATAAAAACTAAAGCA+TGG - chr1_1:60017881-60017900 None:intergenic 30.0%
CACATTACACAGTATACTTT+AGG - chr1_1:60017926-60017945 None:intergenic 30.0%
!!! TGTAATGTGATTTTTTGCAG+TGG + chr1_1:60017937-60017956 Msa0032860:intron 30.0%
!!! GTAATGTGATTTTTTGCAGT+GGG + chr1_1:60017938-60017957 Msa0032860:intron 30.0%
AATTGCTTCTTATCAAAAGC+TGG - chr1_1:60018095-60018114 None:intergenic 30.0%
!! ATTGTAGGTCTTTGAGTTTT+GGG - chr1_1:60018223-60018242 None:intergenic 30.0%
!! AATTGTAGGTCTTTGAGTTT+TGG - chr1_1:60018224-60018243 None:intergenic 30.0%
!!! ATCAATGTTGCAGTTTTGTT+GGG + chr1_1:60018340-60018359 Msa0032860:intron 30.0%
TAAGCAAAGTAGAGAAAAGT+TGG - chr1_1:60018426-60018445 None:intergenic 30.0%
! ACTTTTCTCTACTTTGCTTA+TGG + chr1_1:60018426-60018445 Msa0032860:CDS 30.0%
AAATTGAGAACGGAATGCTT+TGG + chr1_1:60017797-60017816 Msa0032860:exon 35.0%
AATTGAGAACGGAATGCTTT+GGG + chr1_1:60017798-60017817 Msa0032860:exon 35.0%
TTGACATTGTTGACACTTTC+AGG + chr1_1:60018057-60018076 Msa0032860:CDS 35.0%
!! TTGAACAACTCTTGCTTTTC+TGG - chr1_1:60018167-60018186 None:intergenic 35.0%
!! CATCAATGTTGCAGTTTTGT+TGG + chr1_1:60018339-60018358 Msa0032860:intron 35.0%
!!! TCAATGTTGCAGTTTTGTTG+GGG + chr1_1:60018341-60018360 Msa0032860:intron 35.0%
CTTTGTAGTAAGCAAACACA+AGG - chr1_1:60018389-60018408 None:intergenic 35.0%
GTGTTTGCTTACTACAAAGA+TGG + chr1_1:60018390-60018409 Msa0032860:CDS 35.0%
AAATTGAGAACGGAATGCTT+TGG + chr1_1:60017797-60017816 Msa0032860:exon 35.0%
AATTGAGAACGGAATGCTTT+GGG + chr1_1:60017798-60017817 Msa0032860:exon 35.0%
TTGACATTGTTGACACTTTC+AGG + chr1_1:60018057-60018076 Msa0032860:CDS 35.0%
!! TTGAACAACTCTTGCTTTTC+TGG - chr1_1:60018167-60018186 None:intergenic 35.0%
!! CATCAATGTTGCAGTTTTGT+TGG + chr1_1:60018339-60018358 Msa0032860:intron 35.0%
!!! TCAATGTTGCAGTTTTGTTG+GGG + chr1_1:60018341-60018360 Msa0032860:intron 35.0%
CTTTGTAGTAAGCAAACACA+AGG - chr1_1:60018389-60018408 None:intergenic 35.0%
GTGTTTGCTTACTACAAAGA+TGG + chr1_1:60018390-60018409 Msa0032860:CDS 35.0%
CGTTCTCAATTTCCTTGTAG+GGG - chr1_1:60017789-60017808 None:intergenic 40.0%
CCGTTCTCAATTTCCTTGTA+GGG - chr1_1:60017790-60017809 None:intergenic 40.0%
CCCTACAAGGAAATTGAGAA+CGG + chr1_1:60017787-60017806 Msa0032860:five_prime_UTR 40.0%
TCCGTTCTCAATTTCCTTGT+AGG - chr1_1:60017791-60017810 None:intergenic 40.0%
!!! TTTTTGCAGTGGGTTGTTCA+AGG + chr1_1:60017948-60017967 Msa0032860:intron 40.0%
TGATGATCAAGCTGTGAAAG+TGG + chr1_1:60018034-60018053 Msa0032860:CDS 40.0%
GTAGAGAAAAGTTGGATCAG+TGG - chr1_1:60018418-60018437 None:intergenic 40.0%
CTACTTTGCTTATGGCTTGA+AGG + chr1_1:60018434-60018453 Msa0032860:CDS 40.0%
CGTTCTCAATTTCCTTGTAG+GGG - chr1_1:60017789-60017808 None:intergenic 40.0%
CCGTTCTCAATTTCCTTGTA+GGG - chr1_1:60017790-60017809 None:intergenic 40.0%
CCCTACAAGGAAATTGAGAA+CGG + chr1_1:60017787-60017806 Msa0032860:five_prime_UTR 40.0%
TCCGTTCTCAATTTCCTTGT+AGG - chr1_1:60017791-60017810 None:intergenic 40.0%
!!! TTTTTGCAGTGGGTTGTTCA+AGG + chr1_1:60017948-60017967 Msa0032860:intron 40.0%
TGATGATCAAGCTGTGAAAG+TGG + chr1_1:60018034-60018053 Msa0032860:CDS 40.0%
GTAGAGAAAAGTTGGATCAG+TGG - chr1_1:60018418-60018437 None:intergenic 40.0%
CTACTTTGCTTATGGCTTGA+AGG + chr1_1:60018434-60018453 Msa0032860:CDS 40.0%
TGTTGACACTTTCAGGCTTC+AGG + chr1_1:60018064-60018083 Msa0032860:CDS 45.0%
!!! GTAGGTCTTTGAGTTTTGGG+AGG - chr1_1:60018220-60018239 None:intergenic 45.0%
TGTTGACACTTTCAGGCTTC+AGG + chr1_1:60018064-60018083 Msa0032860:CDS 45.0%
!!! GTAGGTCTTTGAGTTTTGGG+AGG - chr1_1:60018220-60018239 None:intergenic 45.0%
CTCTGACTCATTCCCCTACA+AGG + chr1_1:60017774-60017793 Msa0032860:five_prime_UTR 50.0%
!! GAACGGAATGCTTTGGGAAG+TGG + chr1_1:60017804-60017823 Msa0032860:exon 50.0%
!! GGAATGCTTTGGGAAGTGGA+AGG + chr1_1:60017808-60017827 Msa0032860:exon 50.0%
!! GCTTTGGGAAGTGGAAGGAA+AGG + chr1_1:60017813-60017832 Msa0032860:CDS 50.0%
GGGAAGTGGAAGGAAAGGTT+TGG + chr1_1:60017818-60017837 Msa0032860:intron 50.0%
GGAGCAGTGGATGTGAACAT+TGG + chr1_1:60017969-60017988 Msa0032860:CDS 50.0%
! TGGTGCTAATCCTTCTGCTG+AGG + chr1_1:60017989-60018008 Msa0032860:CDS 50.0%
GGTGCTAATCCTTCTGCTGA+GGG + chr1_1:60017990-60018009 Msa0032860:CDS 50.0%
CTCTGACTCATTCCCCTACA+AGG + chr1_1:60017774-60017793 Msa0032860:five_prime_UTR 50.0%
!! GAACGGAATGCTTTGGGAAG+TGG + chr1_1:60017804-60017823 Msa0032860:exon 50.0%
!! GGAATGCTTTGGGAAGTGGA+AGG + chr1_1:60017808-60017827 Msa0032860:exon 50.0%
!! GCTTTGGGAAGTGGAAGGAA+AGG + chr1_1:60017813-60017832 Msa0032860:CDS 50.0%
GGGAAGTGGAAGGAAAGGTT+TGG + chr1_1:60017818-60017837 Msa0032860:intron 50.0%
GGAGCAGTGGATGTGAACAT+TGG + chr1_1:60017969-60017988 Msa0032860:CDS 50.0%
! TGGTGCTAATCCTTCTGCTG+AGG + chr1_1:60017989-60018008 Msa0032860:CDS 50.0%
GGTGCTAATCCTTCTGCTGA+GGG + chr1_1:60017990-60018009 Msa0032860:CDS 50.0%
! GTGGGTTGTTCAAGGAGCAG+TGG + chr1_1:60017956-60017975 Msa0032860:intron 55.0%
GCTAATCCTTCTGCTGAGGG+CGG + chr1_1:60017993-60018012 Msa0032860:CDS 55.0%
! GGGGAGAGCATGCATGATGA+TGG + chr1_1:60018360-60018379 Msa0032860:CDS 55.0%
! GTGGGTTGTTCAAGGAGCAG+TGG + chr1_1:60017956-60017975 Msa0032860:intron 55.0%
GCTAATCCTTCTGCTGAGGG+CGG + chr1_1:60017993-60018012 Msa0032860:CDS 55.0%
! GGGGAGAGCATGCATGATGA+TGG + chr1_1:60018360-60018379 Msa0032860:CDS 55.0%
TCATCTCCGCCCTCAGCAGA+AGG - chr1_1:60018002-60018021 None:intergenic 60.0%
TTCTGCTGAGGGCGGAGATG+AGG + chr1_1:60018001-60018020 Msa0032860:CDS 60.0%
TCATCTCCGCCCTCAGCAGA+AGG - chr1_1:60018002-60018021 None:intergenic 60.0%
TTCTGCTGAGGGCGGAGATG+AGG + chr1_1:60018001-60018020 Msa0032860:CDS 60.0%
GAGGGCGGAGATGAGGACGA+CGG + chr1_1:60018008-60018027 Msa0032860:CDS 65.0%
GAGGGCGGAGATGAGGACGA+CGG + chr1_1:60018008-60018027 Msa0032860:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 60017715 60018623 60017715 ID=Msa0032860;Name=Msa0032860
chr1_1 mRNA 60017715 60018623 60017715 ID=Msa0032860-mRNA-1;Parent=Msa0032860;Name=Msa0032860-mRNA-1;_AED=0.06;_eAED=0.06;_QI=96|1|1|1|1|1|3|153|142
chr1_1 exon 60017715 60017834 60017715 ID=Msa0032860-mRNA-1:exon:18689;Parent=Msa0032860-mRNA-1
chr1_1 exon 60017957 60018243 60017957 ID=Msa0032860-mRNA-1:exon:18690;Parent=Msa0032860-mRNA-1
chr1_1 exon 60018353 60018623 60018353 ID=Msa0032860-mRNA-1:exon:18691;Parent=Msa0032860-mRNA-1
chr1_1 five_prime_UTR 60017715 60017810 60017715 ID=Msa0032860-mRNA-1:five_prime_utr;Parent=Msa0032860-mRNA-1
chr1_1 CDS 60017811 60017834 60017811 ID=Msa0032860-mRNA-1:cds;Parent=Msa0032860-mRNA-1
chr1_1 CDS 60017957 60018243 60017957 ID=Msa0032860-mRNA-1:cds;Parent=Msa0032860-mRNA-1
chr1_1 CDS 60018353 60018470 60018353 ID=Msa0032860-mRNA-1:cds;Parent=Msa0032860-mRNA-1
chr1_1 three_prime_UTR 60018471 60018623 60018471 ID=Msa0032860-mRNA-1:three_prime_utr;Parent=Msa0032860-mRNA-1
Gene Sequence

>Msa0032860

ATGCTTTGGGAAGTGGAAGGAAAGTGGGTTGTTCAAGGAGCAGTGGATGTGAACATTGGTGCTAATCCTTCTGCTGAGGGCGGAGATGAGGACGACGGTGTTGATGATCAAGCTGTGAAAGTGGTTGACATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGAAGCAATTTGTTACTTATATGAAGAGATATATTAAGTTGCTGACATCTAAATTAGAGCCAGAAAAGCAAGAGTTGTTCAAGAAGCACATTGAAGCAGCAACTAAGTTTCTCCTCCCAAAACTCAAAGACCTACAATTTTTTGTTGGGGAGAGCATGCATGATGATGGAAGCCTTGTGTTTGCTTACTACAAAGATGGTGCCACTGATCCAACTTTTCTCTACTTTGCTTATGGCTTGAAGGAAATCAAGTGTTAA

Protein sequence

>Msa0032860

MLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVVDIVDTFRLQEQPAFDKKQFVTYMKRYIKLLTSKLEPEKQELFKKHIEAATKFLLPKLKDLQFFVGESMHDDGSLVFAYYKDGATDPTFLYFAYGLKEIKC*