AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1407460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTTCATTACGAGATT+GGG 0.257084 tig0023313:-1509 Msa1407460:intergenic
GCAATCTTCACGTGACAAAT+TGG 0.264557 tig0023313:+1296 Msa1407460:CDS
TATCAATTTCATTACGAGAT+TGG 0.323363 tig0023313:-1510 Msa1407460:intergenic
CTTCTCCTGTGGTTACTATC+TGG 0.332847 tig0023313:+1828 Msa1407460:three_prime_UTR
TGAATATATTACAATTCATA+TGG 0.372172 tig0023313:+1655 Msa1407460:three_prime_UTR
TGATAAGATCAGTTCGTGTT+GGG 0.372555 tig0023313:+1528 Msa1407460:three_prime_UTR
CTTGAATTGCCAGATTTGTA+AGG 0.388407 tig0023313:+1426 Msa1407460:intron
TCCGAGATAGATGTAACTTC+AGG 0.464090 tig0023313:+645 Msa1407460:five_prime_UTR
TGCCAGATTTGTAAGGGAAA+CGG 0.477376 tig0023313:+1433 Msa1407460:intron
TTGATTTGCAGTGAAGACTT+AGG 0.488504 tig0023313:-1855 Msa1407460:intergenic
TTGATAAGATCAGTTCGTGT+TGG 0.501135 tig0023313:+1527 Msa1407460:three_prime_UTR
TCCTGAAGTTACATCTATCT+CGG 0.506559 tig0023313:-646 Msa1407460:intergenic
TCCTGTGGTTACTATCTGGT+CGG 0.519006 tig0023313:+1832 Msa1407460:three_prime_UTR
TTGAATTGCCAGATTTGTAA+GGG 0.539256 tig0023313:+1427 Msa1407460:intron
CAAGAAAAGAAATGACTGGT+TGG 0.553193 tig0023313:-1576 Msa1407460:intergenic
TCAATTTCATTACGAGATTG+GGG 0.561595 tig0023313:-1508 Msa1407460:intergenic
CCATCAAGAAAAGAAATGAC+TGG 0.584636 tig0023313:-1580 Msa1407460:intergenic
GATAAGATCAGTTCGTGTTG+GGG 0.587224 tig0023313:+1529 Msa1407460:three_prime_UTR
GGTGAAAGCAGCATCCCTGT+TGG 0.615648 tig0023313:+1458 Msa1407460:three_prime_UTR
TCTTGATGGAGGTGAGCGCG+AGG 0.629773 tig0023313:+1594 Msa1407460:three_prime_UTR
ATTGCAATGTGTAGATTGAG+AGG 0.640221 tig0023313:-1278 Msa1407460:intergenic
CTTCACGTGACAAATTGGAA+AGG 0.642563 tig0023313:+1301 Msa1407460:CDS
AGATTTGTAAGGGAAACGGT+TGG 0.649799 tig0023313:+1437 Msa1407460:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGATATATTTATAAAGAT+GGG + tig0023313:716-735 Msa1407460:intron 10.0%
!!! TTTTGGAAAAAAATAAATTA+TGG - tig0023313:1384-1403 Msa1407460:intergenic 10.0%
!! ATTGATATATTTATAAAGAT+GGG + tig0023313:716-735 Msa1407460:intron 10.0%
!!! TTTTGGAAAAAAATAAATTA+TGG - tig0023313:1384-1403 Msa1407460:intergenic 10.0%
!! CATTGATATATTTATAAAGA+TGG + tig0023313:715-734 Msa1407460:intron 15.0%
!! TTGATATATTTATAAAGATG+GGG + tig0023313:717-736 Msa1407460:intron 15.0%
!! CAATATTATAAATAAATAGC+AGG - tig0023313:1221-1240 Msa1407460:intergenic 15.0%
!! TTATTTATAATATTGTCCAA+TGG + tig0023313:1226-1245 Msa1407460:intron 15.0%
!! AAATAAATTATGGTTGTAAA+TGG - tig0023313:1374-1393 Msa1407460:intergenic 15.0%
!! TGAATATATTACAATTCATA+TGG + tig0023313:1655-1674 Msa1407460:three_prime_UTR 15.0%
!! CATTGATATATTTATAAAGA+TGG + tig0023313:715-734 Msa1407460:intron 15.0%
!! TTGATATATTTATAAAGATG+GGG + tig0023313:717-736 Msa1407460:intron 15.0%
!! CAATATTATAAATAAATAGC+AGG - tig0023313:1221-1240 Msa1407460:intergenic 15.0%
!! TTATTTATAATATTGTCCAA+TGG + tig0023313:1226-1245 Msa1407460:intron 15.0%
!! AAATAAATTATGGTTGTAAA+TGG - tig0023313:1374-1393 Msa1407460:intergenic 15.0%
!! TGAATATATTACAATTCATA+TGG + tig0023313:1655-1674 Msa1407460:three_prime_UTR 15.0%
!! CATTGAATAAATTATGTATG+TGG - tig0023313:868-887 Msa1407460:intergenic 20.0%
!!! TTTCAAGTAATTTCATTCAT+CGG - tig0023313:1119-1138 Msa1407460:intergenic 20.0%
!! ATTCAAGATGAAGAAATTAT+GGG - tig0023313:1413-1432 Msa1407460:intergenic 20.0%
!! AATTCAAGATGAAGAAATTA+TGG - tig0023313:1414-1433 Msa1407460:intergenic 20.0%
!!! GTTTTGATTTGTTTTTGTAT+AGG - tig0023313:1722-1741 Msa1407460:intergenic 20.0%
!! CATTGAATAAATTATGTATG+TGG - tig0023313:868-887 Msa1407460:intergenic 20.0%
!!! TTTCAAGTAATTTCATTCAT+CGG - tig0023313:1119-1138 Msa1407460:intergenic 20.0%
!! ATTCAAGATGAAGAAATTAT+GGG - tig0023313:1413-1432 Msa1407460:intergenic 20.0%
!! AATTCAAGATGAAGAAATTA+TGG - tig0023313:1414-1433 Msa1407460:intergenic 20.0%
!!! GTTTTGATTTGTTTTTGTAT+AGG - tig0023313:1722-1741 Msa1407460:intergenic 20.0%
!! TCAGGATTTTATGTTCTATT+TGG + tig0023313:663-682 Msa1407460:exon 25.0%
!!! TTTTATGTTCTATTTGGTTC+AGG + tig0023313:669-688 Msa1407460:exon 25.0%
! TGTTTATAATTGACACTAGA+GGG - tig0023313:814-833 Msa1407460:intergenic 25.0%
! TTGTTTATAATTGACACTAG+AGG - tig0023313:815-834 Msa1407460:intergenic 25.0%
!! AATTTGCTTATAATTGAGAC+CGG + tig0023313:903-922 Msa1407460:intron 25.0%
!! TATGGTGAGTGATATTTTAT+TGG - tig0023313:948-967 Msa1407460:intergenic 25.0%
! GATTTGACTAATACGATTTA+AGG + tig0023313:1084-1103 Msa1407460:intron 25.0%
! AAACATTCTATTTCAAAGCA+AGG + tig0023313:1136-1155 Msa1407460:intron 25.0%
!!! GAAATTATGGGTTTGTTTTT+TGG - tig0023313:1401-1420 Msa1407460:intergenic 25.0%
! ATCAATTTCATTACGAGATT+GGG - tig0023313:1512-1531 Msa1407460:intergenic 25.0%
! TATCAATTTCATTACGAGAT+TGG - tig0023313:1513-1532 Msa1407460:intergenic 25.0%
!! TCAGGATTTTATGTTCTATT+TGG + tig0023313:663-682 Msa1407460:exon 25.0%
!!! TTTTATGTTCTATTTGGTTC+AGG + tig0023313:669-688 Msa1407460:exon 25.0%
! TGTTTATAATTGACACTAGA+GGG - tig0023313:814-833 Msa1407460:intergenic 25.0%
! TTGTTTATAATTGACACTAG+AGG - tig0023313:815-834 Msa1407460:intergenic 25.0%
!! AATTTGCTTATAATTGAGAC+CGG + tig0023313:903-922 Msa1407460:intron 25.0%
!! TATGGTGAGTGATATTTTAT+TGG - tig0023313:948-967 Msa1407460:intergenic 25.0%
! GATTTGACTAATACGATTTA+AGG + tig0023313:1084-1103 Msa1407460:intron 25.0%
! AAACATTCTATTTCAAAGCA+AGG + tig0023313:1136-1155 Msa1407460:intron 25.0%
!!! GAAATTATGGGTTTGTTTTT+TGG - tig0023313:1401-1420 Msa1407460:intergenic 25.0%
! ATCAATTTCATTACGAGATT+GGG - tig0023313:1512-1531 Msa1407460:intergenic 25.0%
! TATCAATTTCATTACGAGAT+TGG - tig0023313:1513-1532 Msa1407460:intergenic 25.0%
TGTGAGTATTTCACTCATAT+TGG + tig0023313:768-787 Msa1407460:intron 30.0%
TCTTCTTTATTAGTACTCTC+CGG - tig0023313:925-944 Msa1407460:intergenic 30.0%
TATATCTGTCATCACCAAAT+TGG - tig0023313:1036-1055 Msa1407460:intergenic 30.0%
! GGTATTTTATTCCTTCTTCA+AGG + tig0023313:1322-1341 Msa1407460:intron 30.0%
TTGAATTGCCAGATTTGTAA+GGG + tig0023313:1427-1446 Msa1407460:intron 30.0%
TCAATTTCATTACGAGATTG+GGG - tig0023313:1511-1530 Msa1407460:intergenic 30.0%
! ATGATTATTTTCTTCTCCTG+TGG + tig0023313:1817-1836 Msa1407460:three_prime_UTR 30.0%
TGTGAGTATTTCACTCATAT+TGG + tig0023313:768-787 Msa1407460:intron 30.0%
TCTTCTTTATTAGTACTCTC+CGG - tig0023313:925-944 Msa1407460:intergenic 30.0%
TATATCTGTCATCACCAAAT+TGG - tig0023313:1036-1055 Msa1407460:intergenic 30.0%
! GGTATTTTATTCCTTCTTCA+AGG + tig0023313:1322-1341 Msa1407460:intron 30.0%
TTGAATTGCCAGATTTGTAA+GGG + tig0023313:1427-1446 Msa1407460:intron 30.0%
TCAATTTCATTACGAGATTG+GGG - tig0023313:1511-1530 Msa1407460:intergenic 30.0%
! ATGATTATTTTCTTCTCCTG+TGG + tig0023313:1817-1836 Msa1407460:three_prime_UTR 30.0%
TCCTGAAGTTACATCTATCT+CGG - tig0023313:649-668 Msa1407460:intergenic 35.0%
TAAAAACAAAGTTGTGCTGC+AGG - tig0023313:1059-1078 Msa1407460:intergenic 35.0%
CATTCTATTTCAAAGCAAGG+CGG + tig0023313:1139-1158 Msa1407460:intron 35.0%
ATTGCAATGTGTAGATTGAG+AGG - tig0023313:1281-1300 Msa1407460:intergenic 35.0%
!! GTTTTCAATAGCCTTGAAGA+AGG - tig0023313:1336-1355 Msa1407460:intergenic 35.0%
CTTGAATTGCCAGATTTGTA+AGG + tig0023313:1426-1445 Msa1407460:intron 35.0%
TTGATAAGATCAGTTCGTGT+TGG + tig0023313:1527-1546 Msa1407460:three_prime_UTR 35.0%
TGATAAGATCAGTTCGTGTT+GGG + tig0023313:1528-1547 Msa1407460:three_prime_UTR 35.0%
CAAGAAAAGAAATGACTGGT+TGG - tig0023313:1579-1598 Msa1407460:intergenic 35.0%
CCATCAAGAAAAGAAATGAC+TGG - tig0023313:1583-1602 Msa1407460:intergenic 35.0%
! CCAGTCATTTCTTTTCTTGA+TGG + tig0023313:1580-1599 Msa1407460:three_prime_UTR 35.0%
! GTCATTTCTTTTCTTGATGG+AGG + tig0023313:1583-1602 Msa1407460:three_prime_UTR 35.0%
TCCTGAAGTTACATCTATCT+CGG - tig0023313:649-668 Msa1407460:intergenic 35.0%
TAAAAACAAAGTTGTGCTGC+AGG - tig0023313:1059-1078 Msa1407460:intergenic 35.0%
CATTCTATTTCAAAGCAAGG+CGG + tig0023313:1139-1158 Msa1407460:intron 35.0%
ATTGCAATGTGTAGATTGAG+AGG - tig0023313:1281-1300 Msa1407460:intergenic 35.0%
!! GTTTTCAATAGCCTTGAAGA+AGG - tig0023313:1336-1355 Msa1407460:intergenic 35.0%
CTTGAATTGCCAGATTTGTA+AGG + tig0023313:1426-1445 Msa1407460:intron 35.0%
TTGATAAGATCAGTTCGTGT+TGG + tig0023313:1527-1546 Msa1407460:three_prime_UTR 35.0%
TGATAAGATCAGTTCGTGTT+GGG + tig0023313:1528-1547 Msa1407460:three_prime_UTR 35.0%
CAAGAAAAGAAATGACTGGT+TGG - tig0023313:1579-1598 Msa1407460:intergenic 35.0%
CCATCAAGAAAAGAAATGAC+TGG - tig0023313:1583-1602 Msa1407460:intergenic 35.0%
! CCAGTCATTTCTTTTCTTGA+TGG + tig0023313:1580-1599 Msa1407460:three_prime_UTR 35.0%
! GTCATTTCTTTTCTTGATGG+AGG + tig0023313:1583-1602 Msa1407460:three_prime_UTR 35.0%
TCCGAGATAGATGTAACTTC+AGG + tig0023313:645-664 Msa1407460:five_prime_UTR 40.0%
ATCACCAAATTGGTGTGTCT+GGG - tig0023313:1026-1045 Msa1407460:intergenic 40.0%
ATTGTCCAATGGATATGCTC+AGG + tig0023313:1237-1256 Msa1407460:intron 40.0%
TTGTCCAATGGATATGCTCA+GGG + tig0023313:1238-1257 Msa1407460:intron 40.0%
GCAATCTTCACGTGACAAAT+TGG + tig0023313:1296-1315 Msa1407460:CDS 40.0%
CTTCACGTGACAAATTGGAA+AGG + tig0023313:1301-1320 Msa1407460:CDS 40.0%
TGCCAGATTTGTAAGGGAAA+CGG + tig0023313:1433-1452 Msa1407460:intron 40.0%
AACCGTTTCCCTTACAAATC+TGG - tig0023313:1438-1457 Msa1407460:intergenic 40.0%
AGATTTGTAAGGGAAACGGT+TGG + tig0023313:1437-1456 Msa1407460:intron 40.0%
!! CTTTTGCTATCTCTCCAACA+GGG - tig0023313:1475-1494 Msa1407460:intergenic 40.0%
!! ACTTTTGCTATCTCTCCAAC+AGG - tig0023313:1476-1495 Msa1407460:intergenic 40.0%
GATAAGATCAGTTCGTGTTG+GGG + tig0023313:1529-1548 Msa1407460:three_prime_UTR 40.0%
TCCGAGATAGATGTAACTTC+AGG + tig0023313:645-664 Msa1407460:five_prime_UTR 40.0%
ATCACCAAATTGGTGTGTCT+GGG - tig0023313:1026-1045 Msa1407460:intergenic 40.0%
ATTGTCCAATGGATATGCTC+AGG + tig0023313:1237-1256 Msa1407460:intron 40.0%
TTGTCCAATGGATATGCTCA+GGG + tig0023313:1238-1257 Msa1407460:intron 40.0%
GCAATCTTCACGTGACAAAT+TGG + tig0023313:1296-1315 Msa1407460:CDS 40.0%
CTTCACGTGACAAATTGGAA+AGG + tig0023313:1301-1320 Msa1407460:CDS 40.0%
TGCCAGATTTGTAAGGGAAA+CGG + tig0023313:1433-1452 Msa1407460:intron 40.0%
AACCGTTTCCCTTACAAATC+TGG - tig0023313:1438-1457 Msa1407460:intergenic 40.0%
AGATTTGTAAGGGAAACGGT+TGG + tig0023313:1437-1456 Msa1407460:intron 40.0%
!! CTTTTGCTATCTCTCCAACA+GGG - tig0023313:1475-1494 Msa1407460:intergenic 40.0%
!! ACTTTTGCTATCTCTCCAAC+AGG - tig0023313:1476-1495 Msa1407460:intergenic 40.0%
GATAAGATCAGTTCGTGTTG+GGG + tig0023313:1529-1548 Msa1407460:three_prime_UTR 40.0%
!! TGGGGTATTTTACCAGTCCA+TGG + tig0023313:735-754 Msa1407460:intron 45.0%
ACTCACAAGTCACTTGTCCA+TGG - tig0023313:755-774 Msa1407460:intergenic 45.0%
TCACCAAATTGGTGTGTCTG+GGG - tig0023313:1025-1044 Msa1407460:intergenic 45.0%
CATCACCAAATTGGTGTGTC+TGG - tig0023313:1027-1046 Msa1407460:intergenic 45.0%
CTTCTCCTGTGGTTACTATC+TGG + tig0023313:1828-1847 Msa1407460:three_prime_UTR 45.0%
TCCTGTGGTTACTATCTGGT+CGG + tig0023313:1832-1851 Msa1407460:three_prime_UTR 45.0%
!! TGGGGTATTTTACCAGTCCA+TGG + tig0023313:735-754 Msa1407460:intron 45.0%
ACTCACAAGTCACTTGTCCA+TGG - tig0023313:755-774 Msa1407460:intergenic 45.0%
TCACCAAATTGGTGTGTCTG+GGG - tig0023313:1025-1044 Msa1407460:intergenic 45.0%
CATCACCAAATTGGTGTGTC+TGG - tig0023313:1027-1046 Msa1407460:intergenic 45.0%
CTTCTCCTGTGGTTACTATC+TGG + tig0023313:1828-1847 Msa1407460:three_prime_UTR 45.0%
TCCTGTGGTTACTATCTGGT+CGG + tig0023313:1832-1851 Msa1407460:three_prime_UTR 45.0%
!! TAAAATTATTTATCTAATTA+TGG - tig0023313:966-985 Msa1407460:intergenic 5.0%
!! TAAAATTATTTATCTAATTA+TGG - tig0023313:966-985 Msa1407460:intergenic 5.0%
CAAGTCACTTGTCCATGGAC+TGG - tig0023313:750-769 Msa1407460:intergenic 50.0%
AGAGCCCTGAGCATATCCAT+TGG - tig0023313:1245-1264 Msa1407460:intergenic 50.0%
CAAGTCACTTGTCCATGGAC+TGG - tig0023313:750-769 Msa1407460:intergenic 50.0%
AGAGCCCTGAGCATATCCAT+TGG - tig0023313:1245-1264 Msa1407460:intergenic 50.0%
CTGCCCCAGACACACCAATT+TGG + tig0023313:1019-1038 Msa1407460:intron 55.0%
GGTGAAAGCAGCATCCCTGT+TGG + tig0023313:1458-1477 Msa1407460:three_prime_UTR 55.0%
GCCGACCAGATAGTAACCAC+AGG - tig0023313:1836-1855 Msa1407460:intergenic 55.0%
CTGCCCCAGACACACCAATT+TGG + tig0023313:1019-1038 Msa1407460:intron 55.0%
GGTGAAAGCAGCATCCCTGT+TGG + tig0023313:1458-1477 Msa1407460:three_prime_UTR 55.0%
GCCGACCAGATAGTAACCAC+AGG - tig0023313:1836-1855 Msa1407460:intergenic 55.0%
TCTTGATGGAGGTGAGCGCG+AGG + tig0023313:1594-1613 Msa1407460:three_prime_UTR 60.0%
TCTTGATGGAGGTGAGCGCG+AGG + tig0023313:1594-1613 Msa1407460:three_prime_UTR 60.0%
! TGGTGTGTCTGGGGCAGACG+TGG - tig0023313:1016-1035 Msa1407460:intergenic 65.0%
! TGGTGTGTCTGGGGCAGACG+TGG - tig0023313:1016-1035 Msa1407460:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0023313 gene 635 1868 635 ID=Msa1407460;Name=Msa1407460
tig0023313 mRNA 635 1868 635 ID=Msa1407460-mRNA-1;Parent=Msa1407460;Name=Msa1407460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=38|1|0.66|1|1|1|3|422|29
tig0023313 exon 635 690 635 ID=Msa1407460-mRNA-1:exon:25100;Parent=Msa1407460-mRNA-1
tig0023313 exon 1259 1322 1259 ID=Msa1407460-mRNA-1:exon:25101;Parent=Msa1407460-mRNA-1
tig0023313 exon 1439 1868 1439 ID=Msa1407460-mRNA-1:exon:25102;Parent=Msa1407460-mRNA-1
tig0023313 five_prime_UTR 635 672 635 ID=Msa1407460-mRNA-1:five_prime_utr;Parent=Msa1407460-mRNA-1
tig0023313 CDS 673 690 673 ID=Msa1407460-mRNA-1:cds;Parent=Msa1407460-mRNA-1
tig0023313 CDS 1259 1322 1259 ID=Msa1407460-mRNA-1:cds;Parent=Msa1407460-mRNA-1
tig0023313 CDS 1439 1446 1439 ID=Msa1407460-mRNA-1:cds;Parent=Msa1407460-mRNA-1
tig0023313 three_prime_UTR 1447 1868 1447 ID=Msa1407460-mRNA-1:three_prime_utr;Parent=Msa1407460-mRNA-1
Gene Sequence

>Msa1407460

ATGTTCTATTTGGTTCAGGGCTCTTGCTGTCTTTCATCCTCTCAATCTACACATTGCAATCTTCACGTGACAAATTGGAAAGATTTGTAA

Protein sequence

>Msa1407460

MFYLVQGSCCLSSSQSTHCNLHVTNWKDL*