AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0842180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842180 MtrunA17_Chr5g0412151 97.876 518 7 2 1 515 1 517 0.0 1050
Msa0842180 MtrunA17_Chr2g0328971 52.289 415 135 12 11 417 4 363 1.75e-127 382
Msa0842180 MtrunA17_Chr4g0072291 77.523 218 45 2 214 427 148 365 1.05e-120 364
Msa0842180 MtrunA17_Chr7g0249621 62.752 298 77 6 199 462 222 519 3.49e-119 365
Msa0842180 MtrunA17_Chr4g0032631 51.799 417 136 12 10 417 2 362 2.61e-118 359
Msa0842180 MtrunA17_Chr1g0152161 50.380 395 138 10 83 424 126 515 2.20e-114 352
Msa0842180 MtrunA17_Chr8g0343851 53.561 351 124 8 86 407 91 431 1.56e-112 345
Msa0842180 MtrunA17_Chr3g0135161 77.436 195 43 1 216 409 301 495 1.28e-107 335
Msa0842180 MtrunA17_Chr4g0054351 66.667 246 66 4 216 457 301 534 2.41e-105 329
Msa0842180 MtrunA17_Chr5g0400871 67.391 230 68 3 221 443 245 474 1.48e-103 321
Msa0842180 MtrunA17_Chr8g0367311 63.922 255 66 5 262 514 22 252 1.18e-99 300
Msa0842180 MtrunA17_Chr4g0073521 70.482 166 49 0 224 389 46 211 1.25e-81 258
Msa0842180 MtrunA17_Chr8g0355191 68.675 166 52 0 224 389 54 219 1.15e-77 249
Msa0842180 MtrunA17_Chr7g0216601 65.680 169 55 1 224 389 64 232 1.41e-74 241
Msa0842180 MtrunA17_Chr6g0452911 63.743 171 52 1 229 389 65 235 5.68e-73 236
Msa0842180 MtrunA17_Chr8g0355181 67.470 166 54 0 224 389 55 220 1.30e-71 233
Msa0842180 MtrunA17_Chr4g0002011 68.639 169 50 1 224 389 35 203 2.99e-71 230
Msa0842180 MtrunA17_Chr8g0362541 62.222 180 54 1 224 389 68 247 6.36e-66 219
Msa0842180 MtrunA17_Chr2g0305821 65.254 118 41 0 220 337 42 159 6.69e-44 152
Msa0842180 MtrunA17_Chr7g0257361 54.545 176 64 5 179 349 15 179 3.03e-43 158
Msa0842180 MtrunA17_Chr7g0263581 48.438 192 89 4 201 392 114 295 5.21e-40 150
Msa0842180 MtrunA17_Chr4g0053181 52.096 167 66 4 229 392 177 332 5.65e-40 151
Msa0842180 MtrunA17_Chr5g0401891 51.176 170 75 3 229 398 153 314 1.64e-39 150
Msa0842180 MtrunA17_Chr4g0030191 43.304 224 113 5 189 412 96 305 6.59e-39 147
Msa0842180 MtrunA17_Chr2g0325911 43.455 191 84 5 202 392 109 275 1.55e-30 124
Msa0842180 MtrunA17_Chr8g0367321 54.902 102 37 3 164 263 99 193 4.95e-24 99.8
Msa0842180 MtrunA17_Chr2g0305831 71.186 59 17 0 331 389 1 59 3.21e-23 97.8
Msa0842180 MtrunA17_Chr3g0126151 47.368 95 49 1 273 367 47 140 1.43e-21 90.9
Msa0842180 MtrunA17_Chr1g0170761 39.837 123 60 4 225 344 134 245 5.74e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842180 AT5G10510.6 53.213 607 152 23 12 515 7 584 2.18e-161 471
Msa0842180 AT5G10510.4 53.213 607 152 23 12 515 7 584 2.18e-161 471
Msa0842180 AT5G10510.3 51.356 627 153 23 12 515 7 604 7.19e-157 460
Msa0842180 AT5G65510.2 53.578 545 159 17 12 515 7 498 6.62e-155 451
Msa0842180 AT5G65510.1 53.578 545 159 17 12 515 7 498 6.62e-155 451
Msa0842180 AT5G10510.2 56.705 522 128 17 73 515 41 543 1.13e-154 452
Msa0842180 AT5G10510.1 52.605 595 156 22 18 515 4 569 5.34e-154 451
Msa0842180 AT5G10510.7 56.381 525 128 18 73 515 41 546 1.32e-152 447
Msa0842180 AT5G10510.5 56.381 525 128 18 73 515 41 546 1.32e-152 447
Msa0842180 AT5G65510.4 57.940 466 116 14 86 515 19 440 9.21e-150 436
Msa0842180 AT1G51190.1 52.133 422 148 14 11 417 4 386 3.12e-123 372
Msa0842180 AT3G20840.1 50.356 421 156 10 11 421 4 381 7.87e-122 369
Msa0842180 AT5G57390.2 63.929 280 80 6 206 477 63 329 1.72e-118 356
Msa0842180 AT5G57390.1 63.929 280 80 6 206 477 181 447 2.48e-117 357
Msa0842180 AT5G17430.2 67.829 258 77 3 201 454 123 378 6.72e-117 355
Msa0842180 AT5G17430.1 67.829 258 77 3 201 454 185 440 1.48e-116 356
Msa0842180 AT5G65510.3 59.677 310 74 7 83 359 16 307 4.71e-109 327
Msa0842180 AT5G65510.3 35.644 101 50 2 300 392 153 246 2.34e-11 65.5
Msa0842180 AT1G72570.1 65.254 236 70 2 169 401 173 399 9.31e-106 322
Msa0842180 AT4G37750.1 66.812 229 73 1 182 407 237 465 2.81e-104 323
Msa0842180 AT3G54320.3 65.556 180 59 1 224 400 61 240 2.46e-70 231
Msa0842180 AT3G54320.1 65.556 180 59 1 224 400 61 240 2.76e-70 231
Msa0842180 AT1G79700.2 68.047 169 51 1 224 389 48 216 7.19e-62 205
Msa0842180 AT1G16060.1 61.497 187 69 1 206 389 36 222 1.42e-60 203
Msa0842180 AT1G79700.1 63.905 169 48 2 224 389 48 206 6.11e-54 184
Msa0842180 AT3G54320.4 66.187 139 47 0 262 400 36 174 9.31e-52 180
Msa0842180 AT3G54320.5 66.187 139 47 0 262 400 36 174 9.31e-52 180
Msa0842180 AT1G79700.3 68.750 128 40 0 262 389 13 140 6.23e-43 153
Msa0842180 AT1G79700.4 68.750 128 40 0 262 389 7 134 7.18e-43 152
Msa0842180 AT2G41710.1 50.270 185 63 5 215 379 57 232 5.41e-42 155
Msa0842180 AT4G36920.1 46.377 207 103 3 192 398 95 293 6.23e-42 155
Msa0842180 AT4G36920.2 46.377 207 103 3 192 398 95 293 6.23e-42 155
Msa0842180 AT2G41710.4 50.270 185 63 5 215 379 57 232 1.06e-41 155
Msa0842180 AT1G16060.3 66.406 128 43 0 262 389 25 152 3.25e-40 147
Msa0842180 AT2G41710.2 48.947 190 63 6 215 379 57 237 1.23e-39 149
Msa0842180 AT5G67180.2 49.112 169 78 3 229 397 97 257 8.17e-39 144
Msa0842180 AT5G67180.3 49.112 169 78 3 229 397 97 257 1.27e-38 142
Msa0842180 AT5G67180.1 49.419 172 73 4 229 397 97 257 4.22e-38 143
Msa0842180 AT2G28550.3 50.000 164 74 3 229 392 154 309 8.50e-38 145
Msa0842180 AT2G41710.3 42.273 220 63 6 215 379 57 267 2.84e-35 137
Msa0842180 AT3G54320.2 65.657 99 34 0 302 400 1 99 5.06e-31 122
Msa0842180 AT1G16060.2 71.591 88 25 0 302 389 1 88 9.54e-31 119
Msa0842180 AT1G16060.4 71.591 88 25 0 302 389 1 88 9.54e-31 119
Msa0842180 AT1G16060.6 71.591 88 25 0 302 389 1 88 9.54e-31 119
Msa0842180 AT1G16060.5 71.591 88 25 0 302 389 1 88 9.54e-31 119
Msa0842180 AT2G28550.4 39.378 193 102 5 229 418 154 334 3.85e-30 120
Msa0842180 AT5G67180.4 47.552 143 67 3 229 371 97 231 2.21e-29 116
Msa0842180 AT2G28550.1 45.122 164 67 4 229 392 154 294 2.56e-29 120
Msa0842180 AT2G28550.2 47.619 126 52 4 229 351 154 268 9.24e-25 103
Msa0842180 AT2G28550.6 47.619 126 52 4 229 351 154 268 9.24e-25 103
Msa0842180 AT2G39250.2 45.545 101 48 2 229 329 109 202 3.25e-22 95.9
Msa0842180 AT2G28550.5 45.802 131 55 2 262 392 14 128 3.84e-22 97.1
Msa0842180 AT2G39250.1 45.545 101 48 2 229 329 109 202 1.74e-21 95.9
Msa0842180 AT5G60120.6 36.000 200 90 8 225 412 157 330 2.26e-18 88.2
Msa0842180 AT5G60120.1 35.500 200 91 7 225 412 157 330 2.85e-18 88.2
Msa0842180 AT5G60120.2 35.821 201 91 8 225 413 157 331 3.78e-18 87.8
Msa0842180 AT5G60120.4 38.415 164 72 6 225 385 157 294 6.93e-18 86.3
Msa0842180 AT5G60120.3 38.415 164 72 6 225 385 157 294 6.93e-18 86.3
Msa0842180 AT5G60120.5 38.415 164 72 6 225 385 157 294 6.93e-18 86.3
Msa0842180 AT3G54990.2 46.226 106 44 3 229 331 123 218 3.80e-17 81.6
Msa0842180 AT1G16060.7 74.138 58 15 0 332 389 9 66 1.44e-16 78.6
Msa0842180 AT3G54990.1 45.631 103 49 2 229 331 123 218 1.88e-16 81.3
Msa0842180 AT1G36060.1 45.000 80 43 1 310 389 125 203 7.02e-11 63.9

Find 135 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGGCTGAGTTCTATTTA+TGG 0.191348 5_4:+19140040 Msa0842180:CDS
GGCTCTGAAGTTGATGATTC+TGG 0.212206 5_4:+19136005 Msa0842180:CDS
ATTTGCGCCTCGGAACTTTA+TGG 0.216723 5_4:-19139647 None:intergenic
AAGAAGATTGTTGATACTTT+TGG 0.222886 5_4:+19136224 Msa0842180:CDS
GGTGGTGTTGGTTCTGGTGC+TGG 0.238538 5_4:-19135938 None:intergenic
CACGGTAAATTGAAGTTCTT+TGG 0.247591 5_4:-19136247 None:intergenic
GTTGATGATTCTGGTTCAAT+AGG 0.295762 5_4:+19136014 Msa0842180:CDS
TTTGGCTGCTCTAAAGTATT+GGG 0.306366 5_4:+19138364 Msa0842180:CDS
CACCACCACCAGGATCTTAC+TGG 0.307769 5_4:+19135954 Msa0842180:CDS
GAAGATATGAAGCCCATCTA+TGG 0.317062 5_4:+19136785 Msa0842180:CDS
CCTGGTGGTGGTGTTGGTTC+TGG 0.317565 5_4:-19135944 None:intergenic
GCGAGAATTGGCCGTGTTGC+CGG 0.321909 5_4:+19139275 Msa0842180:CDS
TACAGCTGTTATCCCATAGA+TGG 0.324143 5_4:-19136798 None:intergenic
CGTTTGGCTGCTCCGCCAAC+TGG 0.327995 5_4:-19139730 None:intergenic
CGCTGTTGCGGCAGCGGTGG+GGG 0.330171 5_4:+19136118 Msa0842180:CDS
GACAACTTGTATACTCATAA+TGG 0.342173 5_4:+19135510 Msa0842180:CDS
CTGATTCTAGAGAACGCTTT+AGG 0.342313 5_4:-19139753 None:intergenic
CTACAAATTCAAATAATAAC+TGG 0.346786 5_4:+19135379 Msa0842180:CDS
TAGAGAACGCTTTAGGCGTT+TGG 0.354961 5_4:-19139746 None:intergenic
ACCGCTGTTGCGGCAGCGGT+GGG 0.363654 5_4:+19136116 Msa0842180:CDS
CTCCAGTTATGCAAAAGAAT+TGG 0.364311 5_4:+19138961 Msa0842180:CDS
TTGTTCTTGTTCTTCTGCTT+TGG 0.368361 5_4:-19135763 None:intergenic
TCAAGTCAATAGGACTGATT+TGG 0.370990 5_4:-19140063 None:intergenic
GTGGTGTTGGTTCTGGTGCT+GGG 0.381115 5_4:-19135937 None:intergenic
CCTAGAAAATCTTCTAGCTT+TGG 0.385172 5_4:-19135834 None:intergenic
CCTTGTGACACCCCGGTAGA+TGG 0.387528 5_4:-19139143 None:intergenic
CTTTATGGCCGCAATGTCAT+AGG 0.388549 5_4:-19139632 None:intergenic
GAGATACAAGTCTTTGTTCC+CGG 0.390452 5_4:-19139294 None:intergenic
TTCATGCAGGCATCACCAAC+AGG 0.405289 5_4:+19139241 Msa0842180:intron
CTCCACCGCTGTTGCGGCAG+CGG 0.407318 5_4:+19136112 Msa0842180:CDS
GCTGCATGTGGGAATGAGTT+TGG 0.416652 5_4:+19136050 Msa0842180:CDS
GGTAGGTGGCAAGCGAGAAT+TGG 0.417872 5_4:+19139263 Msa0842180:CDS
CACCGCTGTTGCGGCAGCGG+TGG 0.419268 5_4:+19136115 Msa0842180:CDS
TCTGGTTCAATAGGGAAATC+AGG 0.420422 5_4:+19136023 Msa0842180:CDS
GGCCGCAAGATCCTATGATT+TGG 0.422462 5_4:+19138346 Msa0842180:CDS
CAGCAACAACATCAACTTCT+CGG 0.424028 5_4:+19139855 Msa0842180:CDS
ATTTGGCTGCTCTAAAGTAT+TGG 0.427354 5_4:+19138363 Msa0842180:CDS
TATGGGATAACAGCTGTAGA+AGG 0.428845 5_4:+19136803 Msa0842180:CDS
AAACACTACTTCAAGTCAAT+AGG 0.436256 5_4:-19140073 None:intergenic
GACTTGTATCTCGGAACATT+CGG 0.436559 5_4:+19139305 Msa0842180:CDS
GTTGGTTCTGGTGCTGGGAG+TGG 0.437052 5_4:-19135932 None:intergenic
GTTGTTGCTGCTGCTGCTGT+TGG 0.444214 5_4:-19139842 None:intergenic
CGAGAATTGGCCGTGTTGCC+GGG 0.446110 5_4:+19139276 Msa0842180:CDS
CTTTAGAGCAGCCAAATCAT+AGG 0.447422 5_4:-19138357 None:intergenic
AGAACAATCAATCTGGTTTG+TGG 0.454287 5_4:+19135779 Msa0842180:CDS
TGATTCTGCAGCGCCATCAT+TGG 0.461546 5_4:-19139983 None:intergenic
AGTTTGCAGCACTTTGTTGA+GGG 0.462380 5_4:-19139816 None:intergenic
CATTGGTTGCTGATGTTGAA+TGG 0.463694 5_4:-19139878 None:intergenic
ATCATCATCACTACTTTGGT+GGG 0.463957 5_4:+19135907 Msa0842180:CDS
TTGATGATTCTGGTTCAATA+GGG 0.466762 5_4:+19136015 Msa0842180:CDS
CAAGAGGAGCTTCCATCTAC+CGG 0.470693 5_4:+19139131 Msa0842180:CDS
AAGTTTGCAGCACTTTGTTG+AGG 0.477979 5_4:-19139817 None:intergenic
AAGAACAAGAACAATCAATC+TGG 0.483167 5_4:+19135772 Msa0842180:CDS
TATGATCATCATCACTACTT+TGG 0.486383 5_4:+19135903 Msa0842180:CDS
AGAAAATCTTCTAGCTTTGG+TGG 0.488892 5_4:-19135831 None:intergenic
ATGAGAGGAGTTAGTAGTAA+CGG 0.489643 5_4:-19135463 None:intergenic
AAGGGAAGGTCAAGCTAGGA+AGG 0.489953 5_4:+19136822 Msa0842180:CDS
TGGATATGACAAAGAAGAAA+AGG 0.490955 5_4:+19138325 Msa0842180:CDS
TTGTCACTGCATTTGCGCCT+CGG 0.493838 5_4:-19139657 None:intergenic
CAATTTACCGTGGTGTCACT+AGG 0.494957 5_4:+19136258 Msa0842180:CDS
CCAGAACCAACACCACCACC+AGG 0.500484 5_4:+19135944 Msa0842180:CDS
CACCGCTGCCGCAACAGCGG+TGG 0.502764 5_4:-19136114 None:intergenic
TTTCATGCTAGTACCAATGA+TGG 0.504563 5_4:+19139970 Msa0842180:CDS
CCCCACCGCTGCCGCAACAG+CGG 0.507019 5_4:-19136117 None:intergenic
TAAGATCCTGGTGGTGGTGT+TGG 0.509685 5_4:-19135950 None:intergenic
CCGCTGTTGCGGCAGCGGTG+GGG 0.510199 5_4:+19136117 Msa0842180:CDS
TTGGCTGCTCTAAAGTATTG+GGG 0.511428 5_4:+19138365 Msa0842180:CDS
TGGTACTAGCATGAAAATGC+TGG 0.516205 5_4:-19139963 None:intergenic
GATCATCATCACTACTTTGG+TGG 0.521100 5_4:+19135906 Msa0842180:CDS
AGTGACTCTTACAGGGAAGT+TGG 0.524758 5_4:-19138402 None:intergenic
AAATCTTCTAGCTTTGGTGG+TGG 0.525839 5_4:-19135828 None:intergenic
TTTATACTAGGGTGGGGAAA+CGG 0.529987 5_4:+19135738 Msa0842180:intron
CTCAGCCGATGTTGGTGGCA+TGG 0.530259 5_4:-19140028 None:intergenic
AACTGAAGAGGAAGCTGCAG+AGG 0.531137 5_4:+19139609 Msa0842180:CDS
CTTGAAAACCAGTAAGATCC+TGG 0.531357 5_4:-19135962 None:intergenic
TCGCTTGCCACCTACCCTGT+TGG 0.531381 5_4:-19139256 None:intergenic
CTAGCTTTGGTGGTGGAGTG+TGG 0.537501 5_4:-19135821 None:intergenic
AATTCATTGCTTCATTGAGA+AGG 0.538177 5_4:+19139011 Msa0842180:CDS
AACAAACTGAGGCATTTCCA+TGG 0.538307 5_4:-19135421 None:intergenic
CCATCTACCGGGGTGTCACA+AGG 0.539236 5_4:+19139143 Msa0842180:CDS
AAGAGCTCTCTTCCAGTTGG+CGG 0.542438 5_4:+19139718 Msa0842180:CDS
GAGTCATACGGAATACCATA+GGG 0.544015 5_4:-19139910 None:intergenic
GCTAATGGAACTCTGTCGCT+TGG 0.545236 5_4:+19136140 Msa0842180:CDS
TAGCATGAAAATGCTGGAAG+AGG 0.547125 5_4:-19139957 None:intergenic
CGAGTCATACGGAATACCAT+AGG 0.549420 5_4:-19139911 None:intergenic
CCAATGGCTTCAATCCCCTA+TGG 0.549466 5_4:+19139895 Msa0842180:CDS
TAGAACTCAGCCGATGTTGG+TGG 0.553420 5_4:-19140033 None:intergenic
AAGAGGAGCTTCCATCTACC+GGG 0.554347 5_4:+19139132 Msa0842180:CDS
AAGATATGAAGCCCATCTAT+GGG 0.555486 5_4:+19136786 Msa0842180:CDS
CCATAGGGGATTGAAGCCAT+TGG 0.565801 5_4:-19139895 None:intergenic
TGGCTGCTCTAAAGTATTGG+GGG 0.567440 5_4:+19138366 Msa0842180:CDS
AAATAGAACTCAGCCGATGT+TGG 0.570966 5_4:-19140036 None:intergenic
TATGAGTATACAAGTTGTCA+AGG 0.571623 5_4:-19135506 None:intergenic
GTAGAAGGGAAGGTCAAGCT+AGG 0.574052 5_4:+19136818 Msa0842180:CDS
CATGATAACAGCAACTGAAG+AGG 0.574583 5_4:+19139597 Msa0842180:intron
TGCTTACTCCACCGCTGTTG+CGG 0.575574 5_4:+19136106 Msa0842180:CDS
GCTGCAGAATCAGCAGCGAA+TGG 0.575674 5_4:+19139994 Msa0842180:CDS
TAGTAGTAACGGAAGAAGCT+TGG 0.580648 5_4:-19135452 None:intergenic
AAGAGTAGTGGCTTCTCAAG+AGG 0.582094 5_4:+19139115 Msa0842180:CDS
CAATGCCATGCCACCAACAT+CGG 0.582869 5_4:+19140023 Msa0842180:CDS
ACAGCTGTTATCCCATAGAT+GGG 0.584544 5_4:-19136797 None:intergenic
AGGGAAGTTGGTAGTAGCAG+TGG 0.587800 5_4:-19138390 None:intergenic
GGGAACAAAGACTTGTATCT+CGG 0.588471 5_4:+19139296 Msa0842180:CDS
AAATCAGGACAAGCTGCATG+TGG 0.591247 5_4:+19136038 Msa0842180:CDS
GGCATCACCAACAGGGTAGG+TGG 0.595697 5_4:+19139249 Msa0842180:intron
TATTCCGTATGACTCGAATA+CGG 0.597263 5_4:+19139918 Msa0842180:CDS
ATGGGATAACAGCTGTAGAA+GGG 0.605627 5_4:+19136804 Msa0842180:CDS
AGGGAAGGTCAAGCTAGGAA+GGG 0.607036 5_4:+19136823 Msa0842180:CDS
ACAAACTGAGGCATTTCCAT+GGG 0.608871 5_4:-19135420 None:intergenic
ATGAAGAGCTCTCTTCCAGT+TGG 0.609750 5_4:+19139715 Msa0842180:CDS
AGCCAAATCATAGGATCTTG+CGG 0.610099 5_4:-19138348 None:intergenic
TCTTCTTGCAGGAAGAGTAG+TGG 0.611461 5_4:+19139103 Msa0842180:intron
GATAACAGCTGTAGAAGGGA+AGG 0.621863 5_4:+19136808 Msa0842180:CDS
TGCAGAGGCCTATGACATTG+CGG 0.625621 5_4:+19139624 Msa0842180:CDS
CAAGCTAGGAAGGGTCGTCA+AGG 0.628154 5_4:+19136832 Msa0842180:CDS
GCAGAATCAGCAGCGAATGG+AGG 0.629078 5_4:+19139997 Msa0842180:CDS
TGGTGTTGCGCAGAGTTCCG+AGG 0.630317 5_4:+19136160 Msa0842180:CDS
CATTCAACATCAGCAACCAA+TGG 0.631785 5_4:+19139879 Msa0842180:CDS
GCAGGCATCACCAACAGGGT+AGG 0.639876 5_4:+19139246 Msa0842180:intron
AACCAGTAAGATCCTGGTGG+TGG 0.642479 5_4:-19135956 None:intergenic
GGGAAGTTGGTAGTAGCAGT+GGG 0.646966 5_4:-19138389 None:intergenic
AATCAGGACAAGCTGCATGT+GGG 0.650674 5_4:+19136039 Msa0842180:CDS
TAAGCCGTATTCGAGTCATA+CGG 0.655944 5_4:-19139922 None:intergenic
AGTCTTTGTTCCCGGCAACA+CGG 0.656378 5_4:-19139286 None:intergenic
TTACAAACCTTGTGACACCC+CGG 0.662260 5_4:-19139150 None:intergenic
TCATCATCACTACTTTGGTG+GGG 0.667284 5_4:+19135908 Msa0842180:CDS
CAAAGAACTTCAATTTACCG+TGG 0.676500 5_4:+19136248 Msa0842180:CDS
TCATGCAGGCATCACCAACA+GGG 0.679915 5_4:+19139242 Msa0842180:intron
TGGTCATATTGAACAAACTG+AGG 0.687500 5_4:-19135432 None:intergenic
TATTATACCTAGTGACACCA+CGG 0.692652 5_4:-19136265 None:intergenic
ATTGCGGCCATAAAGTTCCG+AGG 0.697657 5_4:+19139640 Msa0842180:CDS
TGAGATGAACAGATATGACG+TGG 0.711855 5_4:+19139684 Msa0842180:CDS
AGAGGAGCTTCCATCTACCG+GGG 0.715037 5_4:+19139133 Msa0842180:CDS
AGTCATACGGAATACCATAG+GGG 0.731791 5_4:-19139909 None:intergenic
GAAAACCAGTAAGATCCTGG+TGG 0.756363 5_4:-19135959 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTATGATTTTAATTTG+GGG + chr5_4:19136739-19136758 Msa0842180:intron 10.0%
!!! AACATACTTTTATTTTATTT+TGG + chr5_4:19137992-19138011 Msa0842180:intron 10.0%
!!! TTAGATTATAAAGTAATTTT+TGG + chr5_4:19138028-19138047 Msa0842180:intron 10.0%
!!! AATTTTATTTCTTAATCTAA+CGG - chr5_4:19138731-19138750 None:intergenic 10.0%
!! TTATTTAATGAATTTCAAAA+TGG - chr5_4:19138857-19138876 None:intergenic 10.0%
!! AAAAACAATAACAATATATA+AGG - chr5_4:19138925-19138944 None:intergenic 10.0%
!!! GTATTTGTTAAAATTTTAAT+AGG - chr5_4:19139051-19139070 None:intergenic 10.0%
!! AACAATAAAAAAAGATATAA+AGG - chr5_4:19139430-19139449 None:intergenic 10.0%
!!! TTGATAAATTGATAATATTA+TGG + chr5_4:19139483-19139502 Msa0842180:intron 10.0%
!! TGTTATAATTAAGATTAAGA+TGG - chr5_4:19135541-19135560 None:intergenic 15.0%
!! AAAAAAACAAATATTCATGT+AGG - chr5_4:19135657-19135676 None:intergenic 15.0%
!! ACAAATAAAAAAAGATCATT+TGG - chr5_4:19136417-19136436 None:intergenic 15.0%
!!! ATAATATATGCTTTTTCATT+TGG + chr5_4:19137082-19137101 Msa0842180:intron 15.0%
!! TGTTACTAGTAAAATTAAAA+GGG + chr5_4:19137338-19137357 Msa0842180:intron 15.0%
!!! TTTATATTGTTTTAGAGATA+TGG + chr5_4:19138261-19138280 Msa0842180:intron 15.0%
!! AAAGATTATTCTTAATTCAA+TGG - chr5_4:19138484-19138503 None:intergenic 15.0%
!!! AAATCCATTTTGAAATTATA+AGG + chr5_4:19138805-19138824 Msa0842180:intron 15.0%
!!! GTTTTTTGTTTAACATTTTT+TGG + chr5_4:19139218-19139237 Msa0842180:intron 15.0%
!! ATATGTATTCTAATACTTTA+AGG - chr5_4:19139342-19139361 None:intergenic 15.0%
!! TATTAGAATACATATATTAC+AGG + chr5_4:19139347-19139366 Msa0842180:intron 15.0%
!!! ATCTTTTTTTATTGTTATGT+AGG + chr5_4:19139434-19139453 Msa0842180:intron 15.0%
!! CTACAAATTCAAATAATAAC+TGG + chr5_4:19135379-19135398 Msa0842180:CDS 20.0%
!! CATAGACATTTCATATATTA+GGG - chr5_4:19135568-19135587 None:intergenic 20.0%
!! ACATAGACATTTCATATATT+AGG - chr5_4:19135569-19135588 None:intergenic 20.0%
!!! TTGTTTTGACTATTTATACT+AGG + chr5_4:19135726-19135745 Msa0842180:intron 20.0%
!!! TGTTTTGACTATTTATACTA+GGG + chr5_4:19135727-19135746 Msa0842180:intron 20.0%
!!! ATTTATTGACTATGTTATGT+TGG + chr5_4:19136596-19136615 Msa0842180:intron 20.0%
!!! GTGTTACTAGTAAAATTAAA+AGG + chr5_4:19137337-19137356 Msa0842180:intron 20.0%
!!! AAAATTTGTCTATTTCTCAT+TGG - chr5_4:19137675-19137694 None:intergenic 20.0%
!!! GACAAATTTTTATCATGTTT+CGG + chr5_4:19137685-19137704 Msa0842180:intron 20.0%
!! TCATGTTAACAAAATACTAT+TGG - chr5_4:19137736-19137755 None:intergenic 20.0%
!!! TATGATTTTTCTTTGTACAT+TGG - chr5_4:19138442-19138461 None:intergenic 20.0%
!! AATGTACAAAGAAAAATCAT+AGG + chr5_4:19138441-19138460 Msa0842180:intron 20.0%
!! CAATCCTTATAATTTCAAAA+TGG - chr5_4:19138812-19138831 None:intergenic 20.0%
!!! TGAATTTCAAAATGGATTTT+AGG - chr5_4:19138849-19138868 None:intergenic 20.0%
!!! TTGTTTTTTTCCTATATCTT+AGG + chr5_4:19138937-19138956 Msa0842180:intron 20.0%
!!! ACATAAAAAGTGTAAATGTA+TGG + chr5_4:19139375-19139394 Msa0842180:intron 20.0%
!!! AATGTATGGTTTTTAAACAA+TGG + chr5_4:19139389-19139408 Msa0842180:intron 20.0%
!!! GATACTATTTAAAATGGTAA+TGG - chr5_4:19139513-19139532 None:intergenic 20.0%
!! TGACAAGATACTATTTAAAA+TGG - chr5_4:19139519-19139538 None:intergenic 20.0%
! AAACAAATATTCATGTAGGT+CGG - chr5_4:19135653-19135672 None:intergenic 25.0%
!! AAGAAGATTGTTGATACTTT+TGG + chr5_4:19136224-19136243 Msa0842180:CDS 25.0%
! TTGTTTGATTGAAAATCCAA+AGG + chr5_4:19136433-19136452 Msa0842180:intron 25.0%
! TCAAGTTCATAGATTTGATT+TGG + chr5_4:19136636-19136655 Msa0842180:intron 25.0%
! AATTAAACAGTGTTTGATTC+TGG + chr5_4:19136921-19136940 Msa0842180:intron 25.0%
! ATTAAACAGTGTTTGATTCT+GGG + chr5_4:19136922-19136941 Msa0842180:intron 25.0%
!!! ATTAATGGTTTTAGAGTTGT+AGG + chr5_4:19136977-19136996 Msa0842180:intron 25.0%
!!! TAGTACTATGATTGATTTGA+TGG + chr5_4:19137017-19137036 Msa0842180:intron 25.0%
! CACAGTTATGAATATTAGTA+CGG - chr5_4:19137306-19137325 None:intergenic 25.0%
!! TACTTTAACTTACACTTATG+AGG + chr5_4:19137466-19137485 Msa0842180:intron 25.0%
!!! GAACATAGAGAAAACTTTTT+TGG - chr5_4:19137643-19137662 None:intergenic 25.0%
! AGAGAGAGAGATAAAATTAA+AGG + chr5_4:19137931-19137950 Msa0842180:intron 25.0%
! GAGAGAGAGATAAAATTAAA+GGG + chr5_4:19137932-19137951 Msa0842180:intron 25.0%
! AAATTTGACTATATGCCATA+AGG + chr5_4:19138056-19138075 Msa0842180:intron 25.0%
! AATTTGACTATATGCCATAA+GGG + chr5_4:19138057-19138076 Msa0842180:intron 25.0%
! AATATCAAATATCACCCTTA+TGG - chr5_4:19138074-19138093 None:intergenic 25.0%
! GTAACATTTGTAAATTGTCA+TGG + chr5_4:19138289-19138308 Msa0842180:intron 25.0%
! TTCTTTGTGTAACAGTTATA+TGG - chr5_4:19138568-19138587 None:intergenic 25.0%
! TATATAAGGTAAGTCTTGAA+AGG - chr5_4:19138911-19138930 None:intergenic 25.0%
! AACAAATACTTATCATGATC+AGG + chr5_4:19139062-19139081 Msa0842180:intron 25.0%
!!! CATTTACACTTTTTATGTCT+TGG - chr5_4:19139373-19139392 None:intergenic 25.0%
TATGAGTATACAAGTTGTCA+AGG - chr5_4:19135509-19135528 None:intergenic 30.0%
GACAACTTGTATACTCATAA+TGG + chr5_4:19135510-19135529 Msa0842180:CDS 30.0%
TTTGACTATTTATACTAGGG+TGG + chr5_4:19135730-19135749 Msa0842180:intron 30.0%
TTGACTATTTATACTAGGGT+GGG + chr5_4:19135731-19135750 Msa0842180:intron 30.0%
AAGAACAAGAACAATCAATC+TGG + chr5_4:19135772-19135791 Msa0842180:CDS 30.0%
TATGATCATCATCACTACTT+TGG + chr5_4:19135903-19135922 Msa0842180:CDS 30.0%
!! TTGATGATTCTGGTTCAATA+GGG + chr5_4:19136015-19136034 Msa0842180:CDS 30.0%
!! GATCTTTTGTTGTCACTATT+TGG + chr5_4:19136455-19136474 Msa0842180:intron 30.0%
!! TAGCTATTGATTTACTCAGT+TGG + chr5_4:19136507-19136526 Msa0842180:intron 30.0%
!!! TTTTAATTTGGGGATTTTGC+AGG + chr5_4:19136749-19136768 Msa0842180:intron 30.0%
ATGCATTGAAGTTGCATTAA+TGG + chr5_4:19136962-19136981 Msa0842180:intron 30.0%
ATAGTACTAATAAGTAACCC+AGG - chr5_4:19137006-19137025 None:intergenic 30.0%
!! CTATGATTGATTTGATGGTA+TGG + chr5_4:19137022-19137041 Msa0842180:intron 30.0%
TTAGTACGGAATTAATCACT+TGG - chr5_4:19137292-19137311 None:intergenic 30.0%
ACAAATTTCAATACAGTGAG+TGG - chr5_4:19137447-19137466 None:intergenic 30.0%
!!! CGGATATGTTTTTTTGACTA+TGG + chr5_4:19137705-19137724 Msa0842180:intron 30.0%
ATGAGTCCTTGTAGTAAAAA+AGG + chr5_4:19137832-19137851 Msa0842180:intron 30.0%
TGAGTCCTTGTAGTAAAAAA+GGG + chr5_4:19137833-19137852 Msa0842180:intron 30.0%
GATAGAAAACTACTGTACAT+TGG - chr5_4:19137889-19137908 None:intergenic 30.0%
AGGGTGATATTTGATATTCT+TGG + chr5_4:19138076-19138095 Msa0842180:intron 30.0%
ATTTGTAAATTGTCATGGCA+TGG + chr5_4:19138294-19138313 Msa0842180:intron 30.0%
TGGATATGACAAAGAAGAAA+AGG + chr5_4:19138325-19138344 Msa0842180:CDS 30.0%
ATATGGTATATGTTCACACA+TGG - chr5_4:19138551-19138570 None:intergenic 30.0%
TGTTACACAAAGAATGTGTT+TGG + chr5_4:19138574-19138593 Msa0842180:intron 30.0%
TGAAAGAAATGTGCTTTACT+TGG + chr5_4:19138599-19138618 Msa0842180:intron 30.0%
CTTGGTACATAAAAATACTG+AGG + chr5_4:19138617-19138636 Msa0842180:intron 30.0%
ACATAAAAATACTGAGGCTA+AGG + chr5_4:19138623-19138642 Msa0842180:intron 30.0%
CTAAGATACCAAATTCACAT+TGG - chr5_4:19138651-19138670 None:intergenic 30.0%
!! TTCAAAATGGATTTTAGGCA+TGG - chr5_4:19138844-19138863 None:intergenic 30.0%
ATAAGAATTGAGACTGTGTA+TGG + chr5_4:19138873-19138892 Msa0842180:intron 30.0%
! TTCCAATTCTTTTGCATAAC+TGG - chr5_4:19138966-19138985 None:intergenic 30.0%
AATTCATTGCTTCATTGAGA+AGG + chr5_4:19139011-19139030 Msa0842180:CDS 30.0%
CATGATCAGGTACTAAAATT+TGG + chr5_4:19139075-19139094 Msa0842180:intron 30.0%
!!! TAACATTTTTTGGTTCATGC+AGG + chr5_4:19139228-19139247 Msa0842180:intron 30.0%
! GTATGGTTTTTAAACAATGG+TGG + chr5_4:19139392-19139411 Msa0842180:intron 30.0%
! ATCATTTTCACTTTCACCCA+TGG + chr5_4:19135404-19135423 Msa0842180:CDS 35.0%
TGGTCATATTGAACAAACTG+AGG - chr5_4:19135435-19135454 None:intergenic 35.0%
ATGAGAGGAGTTAGTAGTAA+CGG - chr5_4:19135466-19135485 None:intergenic 35.0%
!! GTGATGATGATTTTGATGAG+AGG - chr5_4:19135481-19135500 None:intergenic 35.0%
TACTTAACGCCGTTTAACAA+CGG + chr5_4:19135606-19135625 Msa0842180:intron 35.0%
!! CTTTTCTAGCCGTTGTTAAA+CGG - chr5_4:19135618-19135637 None:intergenic 35.0%
TGACTATTTATACTAGGGTG+GGG + chr5_4:19135732-19135751 Msa0842180:intron 35.0%
TTGTTCTTGTTCTTCTGCTT+TGG - chr5_4:19135766-19135785 None:intergenic 35.0%
! AGAACAATCAATCTGGTTTG+TGG + chr5_4:19135779-19135798 Msa0842180:CDS 35.0%
!! AGAAAATCTTCTAGCTTTGG+TGG - chr5_4:19135834-19135853 None:intergenic 35.0%
!! CCTAGAAAATCTTCTAGCTT+TGG - chr5_4:19135837-19135856 None:intergenic 35.0%
! CCAAAGCTAGAAGATTTTCT+AGG + chr5_4:19135834-19135853 Msa0842180:CDS 35.0%
ATCATCATCACTACTTTGGT+GGG + chr5_4:19135907-19135926 Msa0842180:CDS 35.0%
!!! GCTTTTTCAAACAACAACTC+TGG + chr5_4:19135984-19136003 Msa0842180:CDS 35.0%
!! GTTGATGATTCTGGTTCAAT+AGG + chr5_4:19136014-19136033 Msa0842180:CDS 35.0%
! AGCAACAATAGCATTTTCCT+CGG - chr5_4:19136180-19136199 None:intergenic 35.0%
CACGGTAAATTGAAGTTCTT+TGG - chr5_4:19136250-19136269 None:intergenic 35.0%
CAAAGAACTTCAATTTACCG+TGG + chr5_4:19136248-19136267 Msa0842180:CDS 35.0%
TATTATACCTAGTGACACCA+CGG - chr5_4:19136268-19136287 None:intergenic 35.0%
AGTGACAACAAAAGATCCTT+TGG - chr5_4:19136452-19136471 None:intergenic 35.0%
AAGATATGAAGCCCATCTAT+GGG + chr5_4:19136786-19136805 Msa0842180:CDS 35.0%
AACGTTCACTGTTACATGAA+AGG + chr5_4:19137495-19137514 Msa0842180:intron 35.0%
GAGTCCTTGTAGTAAAAAAG+GGG + chr5_4:19137834-19137853 Msa0842180:intron 35.0%
AGTCCTTGTAGTAAAAAAGG+GGG + chr5_4:19137835-19137854 Msa0842180:intron 35.0%
AGACTGTAGATGAGTCAAAA+AGG - chr5_4:19138146-19138165 None:intergenic 35.0%
TGACTCATCTACAGTCTATT+TGG + chr5_4:19138149-19138168 Msa0842180:intron 35.0%
GACTCATCTACAGTCTATTT+GGG + chr5_4:19138150-19138169 Msa0842180:intron 35.0%
!! ATTTGGCTGCTCTAAAGTAT+TGG + chr5_4:19138363-19138382 Msa0842180:CDS 35.0%
!! TTTGGCTGCTCTAAAGTATT+GGG + chr5_4:19138364-19138383 Msa0842180:CDS 35.0%
ACAAAGAAGTGACTCTTACA+GGG - chr5_4:19138412-19138431 None:intergenic 35.0%
AACAAAGAAGTGACTCTTAC+AGG - chr5_4:19138413-19138432 None:intergenic 35.0%
ATCAACTTTACAGTGCAGTT+TGG - chr5_4:19138779-19138798 None:intergenic 35.0%
TAACTGGAGACCTAAGATAT+AGG - chr5_4:19138950-19138969 None:intergenic 35.0%
CTCCAGTTATGCAAAAGAAT+TGG + chr5_4:19138961-19138980 Msa0842180:CDS 35.0%
ATTTGGTTATCTCTTCTTGC+AGG + chr5_4:19139092-19139111 Msa0842180:intron 35.0%
AGTGTACGAATTGTGTTACA+AGG + chr5_4:19139196-19139215 Msa0842180:intron 35.0%
TATTCCGTATGACTCGAATA+CGG + chr5_4:19139918-19139937 Msa0842180:CDS 35.0%
TTTCATGCTAGTACCAATGA+TGG + chr5_4:19139970-19139989 Msa0842180:CDS 35.0%
! ACAAACTGAGGCATTTCCAT+GGG - chr5_4:19135423-19135442 None:intergenic 40.0%
! AACAAACTGAGGCATTTCCA+TGG - chr5_4:19135424-19135443 None:intergenic 40.0%
! TAGTAGTAACGGAAGAAGCT+TGG - chr5_4:19135455-19135474 None:intergenic 40.0%
TTTATACTAGGGTGGGGAAA+CGG + chr5_4:19135738-19135757 Msa0842180:intron 40.0%
!! AAATCTTCTAGCTTTGGTGG+TGG - chr5_4:19135831-19135850 None:intergenic 40.0%
GATCATCATCACTACTTTGG+TGG + chr5_4:19135906-19135925 Msa0842180:CDS 40.0%
TCATCATCACTACTTTGGTG+GGG + chr5_4:19135908-19135927 Msa0842180:CDS 40.0%
CTTGAAAACCAGTAAGATCC+TGG - chr5_4:19135965-19135984 None:intergenic 40.0%
! TCTGGTTCAATAGGGAAATC+AGG + chr5_4:19136023-19136042 Msa0842180:CDS 40.0%
GAAGATATGAAGCCCATCTA+TGG + chr5_4:19136785-19136804 Msa0842180:CDS 40.0%
ACAGCTGTTATCCCATAGAT+GGG - chr5_4:19136800-19136819 None:intergenic 40.0%
TACAGCTGTTATCCCATAGA+TGG - chr5_4:19136801-19136820 None:intergenic 40.0%
TATGGGATAACAGCTGTAGA+AGG + chr5_4:19136803-19136822 Msa0842180:CDS 40.0%
ATGGGATAACAGCTGTAGAA+GGG + chr5_4:19136804-19136823 Msa0842180:CDS 40.0%
!!! TTTTAGAGTTGTAGGACACC+TGG + chr5_4:19136985-19137004 Msa0842180:intron 40.0%
! TTTAGAGTTGTAGGACACCT+GGG + chr5_4:19136986-19137005 Msa0842180:intron 40.0%
ATTCACATGGTGCAGCAAAA+TGG - chr5_4:19137174-19137193 None:intergenic 40.0%
! TTTTGCTGCACCATGTGAAT+TGG + chr5_4:19137174-19137193 Msa0842180:intron 40.0%
TTTGCTGCACCATGTGAATT+GGG + chr5_4:19137175-19137194 Msa0842180:intron 40.0%
CTGAACAGACAAGAAAGCAA+AGG - chr5_4:19137203-19137222 None:intergenic 40.0%
AATACAGTGAGTGGTGTGAT+AGG - chr5_4:19137438-19137457 None:intergenic 40.0%
!!! CAACCCCCTTTTTTACTACA+AGG - chr5_4:19137841-19137860 None:intergenic 40.0%
!! CTCTTTATAGCTTGCTACGT+AGG - chr5_4:19137911-19137930 None:intergenic 40.0%
ATTGCGCATGCAAATGTGAT+TGG + chr5_4:19137967-19137986 Msa0842180:intron 40.0%
!! TTCTTGGCTTTTCCTTTGCA+TGG + chr5_4:19138092-19138111 Msa0842180:intron 40.0%
! ATTGTCATGGCATGGTTTGT+AGG + chr5_4:19138302-19138321 Msa0842180:intron 40.0%
AGCCAAATCATAGGATCTTG+CGG - chr5_4:19138351-19138370 None:intergenic 40.0%
!! CTTTAGAGCAGCCAAATCAT+AGG - chr5_4:19138360-19138379 None:intergenic 40.0%
!! TTGGCTGCTCTAAAGTATTG+GGG + chr5_4:19138365-19138384 Msa0842180:CDS 40.0%
! CTAAGGTGCCAATGTGAATT+TGG + chr5_4:19138640-19138659 Msa0842180:intron 40.0%
GAGATACAAGTCTTTGTTCC+CGG - chr5_4:19139297-19139316 None:intergenic 40.0%
GGGAACAAAGACTTGTATCT+CGG + chr5_4:19139296-19139315 Msa0842180:CDS 40.0%
GACTTGTATCTCGGAACATT+CGG + chr5_4:19139305-19139324 Msa0842180:CDS 40.0%
CATGATAACAGCAACTGAAG+AGG + chr5_4:19139597-19139616 Msa0842180:intron 40.0%
TGAGATGAACAGATATGACG+TGG + chr5_4:19139684-19139703 Msa0842180:CDS 40.0%
! CTGATTCTAGAGAACGCTTT+AGG - chr5_4:19139756-19139775 None:intergenic 40.0%
AGTTTGCAGCACTTTGTTGA+GGG - chr5_4:19139819-19139838 None:intergenic 40.0%
AAGTTTGCAGCACTTTGTTG+AGG - chr5_4:19139820-19139839 None:intergenic 40.0%
CAGCAACAACATCAACTTCT+CGG + chr5_4:19139855-19139874 Msa0842180:CDS 40.0%
! CATTGGTTGCTGATGTTGAA+TGG - chr5_4:19139881-19139900 None:intergenic 40.0%
CATTCAACATCAGCAACCAA+TGG + chr5_4:19139879-19139898 Msa0842180:CDS 40.0%
AGTCATACGGAATACCATAG+GGG - chr5_4:19139912-19139931 None:intergenic 40.0%
GAGTCATACGGAATACCATA+GGG - chr5_4:19139913-19139932 None:intergenic 40.0%
TAAGCCGTATTCGAGTCATA+CGG - chr5_4:19139925-19139944 None:intergenic 40.0%
TAGCATGAAAATGCTGGAAG+AGG - chr5_4:19139960-19139979 None:intergenic 40.0%
TGGTACTAGCATGAAAATGC+TGG - chr5_4:19139966-19139985 None:intergenic 40.0%
AAATAGAACTCAGCCGATGT+TGG - chr5_4:19140039-19140058 None:intergenic 40.0%
! CATCGGCTGAGTTCTATTTA+TGG + chr5_4:19140040-19140059 Msa0842180:CDS 40.0%
GAAAACCAGTAAGATCCTGG+TGG - chr5_4:19135962-19135981 None:intergenic 45.0%
!! GGCTCTGAAGTTGATGATTC+TGG + chr5_4:19136005-19136024 Msa0842180:CDS 45.0%
AAATCAGGACAAGCTGCATG+TGG + chr5_4:19136038-19136057 Msa0842180:CDS 45.0%
AATCAGGACAAGCTGCATGT+GGG + chr5_4:19136039-19136058 Msa0842180:CDS 45.0%
!! CAATTTACCGTGGTGTCACT+AGG + chr5_4:19136258-19136277 Msa0842180:CDS 45.0%
! TTTTGCAGGCATAGATGGAC+AGG + chr5_4:19136763-19136782 Msa0842180:intron 45.0%
GATAACAGCTGTAGAAGGGA+AGG + chr5_4:19136808-19136827 Msa0842180:CDS 45.0%
CCTCTCTCAGAACAAACTCA+CGG + chr5_4:19137531-19137550 Msa0842180:intron 45.0%
CTCTCTCAGAACAAACTCAC+GGG + chr5_4:19137532-19137551 Msa0842180:intron 45.0%
AAAAGCCAGCAATGCACCAA+GGG - chr5_4:19138123-19138142 None:intergenic 45.0%
!! TGGCTGCTCTAAAGTATTGG+GGG + chr5_4:19138366-19138385 Msa0842180:CDS 45.0%
AGTGACTCTTACAGGGAAGT+TGG - chr5_4:19138405-19138424 None:intergenic 45.0%
TCTTCTTGCAGGAAGAGTAG+TGG + chr5_4:19139103-19139122 Msa0842180:intron 45.0%
AAGAGTAGTGGCTTCTCAAG+AGG + chr5_4:19139115-19139134 Msa0842180:CDS 45.0%
TTACAAACCTTGTGACACCC+CGG - chr5_4:19139153-19139172 None:intergenic 45.0%
CTTTATGGCCGCAATGTCAT+AGG - chr5_4:19139635-19139654 None:intergenic 45.0%
ATTTGCGCCTCGGAACTTTA+TGG - chr5_4:19139650-19139669 None:intergenic 45.0%
ATGAAGAGCTCTCTTCCAGT+TGG + chr5_4:19139715-19139734 Msa0842180:CDS 45.0%
! TAGAGAACGCTTTAGGCGTT+TGG - chr5_4:19139749-19139768 None:intergenic 45.0%
CGAGTCATACGGAATACCAT+AGG - chr5_4:19139914-19139933 None:intergenic 45.0%
!!! TTTTATTTATGATTTTAATT+TGG + chr5_4:19136737-19136756 Msa0842180:intron 5.0%
!!! TTTATTTATGATTTTAATTT+GGG + chr5_4:19136738-19136757 Msa0842180:intron 5.0%
!!! TTTTCTATAATATTTTTTTA+TGG + chr5_4:19136873-19136892 Msa0842180:intron 5.0%
!! TAAGATCCTGGTGGTGGTGT+TGG - chr5_4:19135953-19135972 None:intergenic 50.0%
AACCAGTAAGATCCTGGTGG+TGG - chr5_4:19135959-19135978 None:intergenic 50.0%
GCTGCATGTGGGAATGAGTT+TGG + chr5_4:19136050-19136069 Msa0842180:CDS 50.0%
GCTAATGGAACTCTGTCGCT+TGG + chr5_4:19136140-19136159 Msa0842180:CDS 50.0%
! GGGGATTTTGCAGGCATAGA+TGG + chr5_4:19136758-19136777 Msa0842180:intron 50.0%
GTAGAAGGGAAGGTCAAGCT+AGG + chr5_4:19136818-19136837 Msa0842180:CDS 50.0%
AAGGGAAGGTCAAGCTAGGA+AGG + chr5_4:19136822-19136841 Msa0842180:CDS 50.0%
AGGGAAGGTCAAGCTAGGAA+GGG + chr5_4:19136823-19136842 Msa0842180:CDS 50.0%
CACATGGTGCAGCAAAATGG+CGG - chr5_4:19137171-19137190 None:intergenic 50.0%
!! GCAAAGGCACCCAATTCACA+TGG - chr5_4:19137187-19137206 None:intergenic 50.0%
GAGTGGTGTGATAGGTGATG+TGG - chr5_4:19137430-19137449 None:intergenic 50.0%
!! CCGTGAGTTTGTTCTGAGAG+AGG - chr5_4:19137534-19137553 None:intergenic 50.0%
GAAAAGCCAGCAATGCACCA+AGG - chr5_4:19138124-19138143 None:intergenic 50.0%
! GTCATGGCATGGTTTGTAGG+TGG + chr5_4:19138305-19138324 Msa0842180:intron 50.0%
GGCCGCAAGATCCTATGATT+TGG + chr5_4:19138346-19138365 Msa0842180:CDS 50.0%
!! GGGAAGTTGGTAGTAGCAGT+GGG - chr5_4:19138392-19138411 None:intergenic 50.0%
!! AGGGAAGTTGGTAGTAGCAG+TGG - chr5_4:19138393-19138412 None:intergenic 50.0%
CAAGAGGAGCTTCCATCTAC+CGG + chr5_4:19139131-19139150 Msa0842180:CDS 50.0%
AAGAGGAGCTTCCATCTACC+GGG + chr5_4:19139132-19139151 Msa0842180:CDS 50.0%
TTCATGCAGGCATCACCAAC+AGG + chr5_4:19139241-19139260 Msa0842180:intron 50.0%
TCATGCAGGCATCACCAACA+GGG + chr5_4:19139242-19139261 Msa0842180:intron 50.0%
AGTCTTTGTTCCCGGCAACA+CGG - chr5_4:19139289-19139308 None:intergenic 50.0%
AACTGAAGAGGAAGCTGCAG+AGG + chr5_4:19139609-19139628 Msa0842180:CDS 50.0%
TGCAGAGGCCTATGACATTG+CGG + chr5_4:19139624-19139643 Msa0842180:CDS 50.0%
ATTGCGGCCATAAAGTTCCG+AGG + chr5_4:19139640-19139659 Msa0842180:CDS 50.0%
TTGTCACTGCATTTGCGCCT+CGG - chr5_4:19139660-19139679 None:intergenic 50.0%
AAGAGCTCTCTTCCAGTTGG+CGG + chr5_4:19139718-19139737 Msa0842180:CDS 50.0%
CCATAGGGGATTGAAGCCAT+TGG - chr5_4:19139898-19139917 None:intergenic 50.0%
CCAATGGCTTCAATCCCCTA+TGG + chr5_4:19139895-19139914 Msa0842180:CDS 50.0%
TGATTCTGCAGCGCCATCAT+TGG - chr5_4:19139986-19140005 None:intergenic 50.0%
CAATGCCATGCCACCAACAT+CGG + chr5_4:19140023-19140042 Msa0842180:CDS 50.0%
TAGAACTCAGCCGATGTTGG+TGG - chr5_4:19140036-19140055 None:intergenic 50.0%
!! CTAGCTTTGGTGGTGGAGTG+TGG - chr5_4:19135824-19135843 None:intergenic 55.0%
!! GTGGTGTTGGTTCTGGTGCT+GGG - chr5_4:19135940-19135959 None:intergenic 55.0%
CACCACCACCAGGATCTTAC+TGG + chr5_4:19135954-19135973 Msa0842180:CDS 55.0%
TGCTTACTCCACCGCTGTTG+CGG + chr5_4:19136106-19136125 Msa0842180:CDS 55.0%
CAAGCTAGGAAGGGTCGTCA+AGG + chr5_4:19136832-19136851 Msa0842180:CDS 55.0%
AGAGGAGCTTCCATCTACCG+GGG + chr5_4:19139133-19139152 Msa0842180:CDS 55.0%
GGTAGGTGGCAAGCGAGAAT+TGG + chr5_4:19139263-19139282 Msa0842180:CDS 55.0%
GTTGTTGCTGCTGCTGCTGT+TGG - chr5_4:19139845-19139864 None:intergenic 55.0%
GCTGCAGAATCAGCAGCGAA+TGG + chr5_4:19139994-19140013 Msa0842180:CDS 55.0%
GCAGAATCAGCAGCGAATGG+AGG + chr5_4:19139997-19140016 Msa0842180:CDS 55.0%
!! GTTGGTTCTGGTGCTGGGAG+TGG - chr5_4:19135935-19135954 None:intergenic 60.0%
!! GGTGGTGTTGGTTCTGGTGC+TGG - chr5_4:19135941-19135960 None:intergenic 60.0%
!! CCTGGTGGTGGTGTTGGTTC+TGG - chr5_4:19135947-19135966 None:intergenic 60.0%
CCAGAACCAACACCACCACC+AGG + chr5_4:19135944-19135963 Msa0842180:CDS 60.0%
!! TGGTGTTGCGCAGAGTTCCG+AGG + chr5_4:19136160-19136179 Msa0842180:CDS 60.0%
CCAAGGGACGGTCCATGCAA+AGG - chr5_4:19138107-19138126 None:intergenic 60.0%
CCTTTGCATGGACCGTCCCT+TGG + chr5_4:19138104-19138123 Msa0842180:intron 60.0%
! ACCGTCCCTTGGTGCATTGC+TGG + chr5_4:19138115-19138134 Msa0842180:intron 60.0%
GCCAGCAATGCACCAAGGGA+CGG - chr5_4:19138119-19138138 None:intergenic 60.0%
CCTTGTGACACCCCGGTAGA+TGG - chr5_4:19139146-19139165 None:intergenic 60.0%
! CCATCTACCGGGGTGTCACA+AGG + chr5_4:19139143-19139162 Msa0842180:CDS 60.0%
GCAGGCATCACCAACAGGGT+AGG + chr5_4:19139246-19139265 Msa0842180:intron 60.0%
GGCATCACCAACAGGGTAGG+TGG + chr5_4:19139249-19139268 Msa0842180:intron 60.0%
TCGCTTGCCACCTACCCTGT+TGG - chr5_4:19139259-19139278 None:intergenic 60.0%
GCGAGAATTGGCCGTGTTGC+CGG + chr5_4:19139275-19139294 Msa0842180:CDS 60.0%
CGAGAATTGGCCGTGTTGCC+GGG + chr5_4:19139276-19139295 Msa0842180:CDS 60.0%
!! CTCAGCCGATGTTGGTGGCA+TGG - chr5_4:19140031-19140050 None:intergenic 60.0%
CGTTTGGCTGCTCCGCCAAC+TGG - chr5_4:19139733-19139752 None:intergenic 65.0%
CTCCACCGCTGTTGCGGCAG+CGG + chr5_4:19136112-19136131 Msa0842180:CDS 70.0%
ACCGCTGTTGCGGCAGCGGT+GGG + chr5_4:19136116-19136135 Msa0842180:CDS 70.0%
CACCGCTGCCGCAACAGCGG+TGG - chr5_4:19136117-19136136 None:intergenic 75.0%
CACCGCTGTTGCGGCAGCGG+TGG + chr5_4:19136115-19136134 Msa0842180:CDS 75.0%
CCCCACCGCTGCCGCAACAG+CGG - chr5_4:19136120-19136139 None:intergenic 75.0%
CCGCTGTTGCGGCAGCGGTG+GGG + chr5_4:19136117-19136136 Msa0842180:CDS 75.0%
CGCTGTTGCGGCAGCGGTGG+GGG + chr5_4:19136118-19136137 Msa0842180:CDS 75.0%
GCGGCAGCGGTGGGGGCTAA+TGG + chr5_4:19136125-19136144 Msa0842180:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 19135363 19140080 19135363 ID=Msa0842180;Name=Msa0842180
chr5_4 mRNA 19135363 19140080 19135363 ID=Msa0842180-mRNA-1;Parent=Msa0842180;Name=Msa0842180-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.14|0|1|1|1|8|0|515
chr5_4 exon 19135363 19135531 19135363 ID=Msa0842180-mRNA-1:exon:7958;Parent=Msa0842180-mRNA-1
chr5_4 exon 19135748 19136279 19135748 ID=Msa0842180-mRNA-1:exon:7959;Parent=Msa0842180-mRNA-1
chr5_4 exon 19136771 19136853 19136771 ID=Msa0842180-mRNA-1:exon:7960;Parent=Msa0842180-mRNA-1
chr5_4 exon 19138324 19138412 19138324 ID=Msa0842180-mRNA-1:exon:7961;Parent=Msa0842180-mRNA-1
chr5_4 exon 19138959 19139032 19138959 ID=Msa0842180-mRNA-1:exon:7962;Parent=Msa0842180-mRNA-1
chr5_4 exon 19139114 19139164 19139114 ID=Msa0842180-mRNA-1:exon:7963;Parent=Msa0842180-mRNA-1
chr5_4 exon 19139250 19139326 19139250 ID=Msa0842180-mRNA-1:exon:7964;Parent=Msa0842180-mRNA-1
chr5_4 exon 19139608 19140080 19139608 ID=Msa0842180-mRNA-1:exon:7965;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19135363 19135531 19135363 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19135748 19136279 19135748 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19136771 19136853 19136771 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19138324 19138412 19138324 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19138959 19139032 19138959 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19139114 19139164 19139114 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19139250 19139326 19139250 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
chr5_4 CDS 19139608 19140080 19139608 ID=Msa0842180-mRNA-1:cds;Parent=Msa0842180-mRNA-1
Gene Sequence

>Msa0842180

ATGGCTCGTGCTACTACTACAAATTCAAATAATAACTGGCTATCATTTTCACTTTCACCCATGGAAATGCCTCAGTTTGTTCAATATGACCAAGCTTCTTCCGTTACTACTAACTCCTCTCATCAAAATCATCATCACTATTTCCTTGACAACTTGTATACTCATAATGGGTGGGGAAACGGAACCAAAGCAGAAGAACAAGAACAATCAATCTGGTTTGTGGATTCATCAAACACTGTTAACCACACTCCACCACCAAAGCTAGAAGATTTTCTAGGTGACAGTCAAACAGAAACACAAGATTCATCATCACTCACAAACATGTATGATCATCATCACTACTTTGGTGGGGACCACTCCCAGCACCAGAACCAACACCACCACCAGGATCTTACTGGTTTTCAAGCTTTTTCAAACAACAACTCTGGCTCTGAAGTTGATGATTCTGGTTCAATAGGGAAATCAGGACAAGCTGCATGTGGGAATGAGTTTGGAACAACTCATTGTGTTGAGTCTGTGAATGAGTTTGCTTACTCCACCGCTGTTGCGGCAGCGGTGGGGGCTAATGGAACTCTGTCGCTTGGTGTTGCGCAGAGTTCCGAGGAAAATGCTATTGTTGCTGTTGCTGCTGATTCTGATAGCTCTAAGAAGATTGTTGATACTTTTGGCCAAAGAACTTCAATTTACCGTGGTGTCACTAGGCATAGATGGACAGGAAGATATGAAGCCCATCTATGGGATAACAGCTGTAGAAGGGAAGGTCAAGCTAGGAAGGGTCGTCAAGGTGGATATGACAAAGAAGAAAAGGCCGCAAGATCCTATGATTTGGCTGCTCTAAAGTATTGGGGGCCCACTGCTACTACCAACTTCCCTGTCTCCAGTTATGCAAAAGAATTGGAAGAGATGAAGAATGTAACAAAGCAAGAATTCATTGCTTCATTGAGAAGGAAGAGTAGTGGCTTCTCAAGAGGAGCTTCCATCTACCGGGGTGTCACAAGGCATCACCAACAGGGTAGGTGGCAAGCGAGAATTGGCCGTGTTGCCGGGAACAAAGACTTGTATCTCGGAACATTCGCAACTGAAGAGGAAGCTGCAGAGGCCTATGACATTGCGGCCATAAAGTTCCGAGGCGCAAATGCAGTGACAAACTTTGAGATGAACAGATATGACGTGGAAGCGATAATGAAGAGCTCTCTTCCAGTTGGCGGAGCAGCCAAACGCCTAAAGCGTTCTCTAGAATCAGAACAGAAAGCTTCTGCTGACAACAACATTCAACAGAACAACCCTCAACAAAGTGCTGCAAACTTGACCAACAGCAGCAGCAGCAACAACATCAACTTCTCGGCCATTCAACATCAGCAACCAATGGCTTCAATCCCCTATGGTATTCCGTATGACTCGAATACGGCTTACTATCATCACAACCTCTTCCAGCATTTTCATGCTAGTACCAATGATGGCGCTGCAGAATCAGCAGCGAATGGAGGACTCAATGCCATGCCACCAACATCGGCTGAGTTCTATTTATGGCCAAATCAGTCCTATTGA
Protein sequence

>Msa0842180

MARATTTNSNNNWLSFSLSPMEMPQFVQYDQASSVTTNSSHQNHHHYFLDNLYTHNGWGNGTKAEEQEQSIWFVDSSNTVNHTPPPKLEDFLGDSQTETQDSSSLTNMYDHHHYFGGDHSQHQNQHHHQDLTGFQAFSNNNSGSEVDDSGSIGKSGQAACGNEFGTTHCVESVNEFAYSTAVAAAVGANGTLSLGVAQSSEENAIVAVAADSDSSKKIVDTFGQRTSIYRGVTRHRWTGRYEAHLWDNSCRREGQARKGRQGGYDKEEKAARSYDLAALKYWGPTATTNFPVSSYAKELEEMKNVTKQEFIASLRRKSSGFSRGASIYRGVTRHHQQGRWQARIGRVAGNKDLYLGTFATEEEAAEAYDIAAIKFRGANAVTNFEMNRYDVEAIMKSSLPVGGAAKRLKRSLESEQKASADNNIQQNNPQQSAANLTNSSSSNNINFSAIQHQQPMASIPYGIPYDSNTAYYHHNLFQHFHASTNDGAAESAANGGLNAMPPTSAEFYLWPNQSY*