AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1230560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1230560 MtrunA17_Chr8g0339091 91.992 512 21 5 1 506 1 498 0.0 921
Msa1230560 MtrunA17_Chr7g0234761 60.000 545 140 19 1 498 1 514 0.0 549
Msa1230560 MtrunA17_Chr5g0421111 33.913 460 204 13 106 506 140 558 1.84e-58 202
Msa1230560 MtrunA17_Chr3g0144641 42.384 302 145 10 219 506 112 398 2.21e-50 177
Msa1230560 MtrunA17_Chr8g0365941 41.581 291 141 9 105 388 125 393 1.17e-49 177
Msa1230560 MtrunA17_Chr1g0152131 47.368 228 99 7 211 435 181 390 5.56e-47 169
Msa1230560 MtrunA17_Chr4g0035101 50.000 206 81 6 193 396 54 239 2.56e-46 164
Msa1230560 MtrunA17_Chr8g0363921 64.394 132 45 2 250 380 102 232 4.78e-46 162
Msa1230560 MtrunA17_Chr5g0415291 65.289 121 40 1 252 372 67 185 1.04e-45 160
Msa1230560 MtrunA17_Chr3g0131771 62.595 131 43 2 258 385 109 236 9.28e-42 152
Msa1230560 MtrunA17_Chr8g0385911 76.344 93 19 1 296 385 142 234 3.58e-40 147
Msa1230560 MtrunA17_Chr7g0258161 69.318 88 27 0 296 383 170 257 2.60e-37 140
Msa1230560 MtrunA17_Chr1g0174161 55.932 118 46 2 296 413 157 268 7.58e-37 138
Msa1230560 MtrunA17_Chr6g0484271 62.745 102 38 0 278 379 134 235 2.08e-35 133
Msa1230560 MtrunA17_Chr4g0074161 61.446 83 28 2 282 364 192 270 2.55e-22 99.0
Msa1230560 MtrunA17_Chr1g0190731 64.000 75 26 1 290 364 184 257 1.02e-21 96.7
Msa1230560 MtrunA17_Chr1g0182791 39.655 116 56 3 254 369 285 386 2.10e-15 79.0
Msa1230560 MtrunA17_Chr4g0032951 58.462 65 27 0 299 363 355 419 2.49e-15 78.2
Msa1230560 MtrunA17_Chr1g0184841 60.000 75 29 1 290 364 111 184 4.02e-15 75.9
Msa1230560 MtrunA17_Chr2g0329041 50.649 77 31 1 285 361 325 394 4.91e-15 77.0
Msa1230560 MtrunA17_Chr4g0014771 55.385 65 29 0 299 363 118 182 5.22e-15 73.6
Msa1230560 MtrunA17_Chr7g0258181 45.946 74 40 0 299 372 243 316 4.01e-14 73.6
Msa1230560 MtrunA17_Chr1g0193321 32.778 180 97 6 193 364 363 526 4.07e-14 75.1
Msa1230560 MtrunA17_Chr1g0174271 52.239 67 32 0 299 365 298 364 4.69e-14 73.9
Msa1230560 MtrunA17_Chr7g0262691 38.136 118 66 3 257 373 215 326 2.46e-13 72.0
Msa1230560 MtrunA17_Chr3g0087771 50.769 65 32 0 299 363 322 386 5.12e-13 70.9
Msa1230560 MtrunA17_Chr5g0393721 40.625 96 47 2 300 386 225 319 1.33e-12 68.9
Msa1230560 MtrunA17_Chr1g0152651 52.308 65 30 1 299 363 274 337 2.04e-12 68.6
Msa1230560 MtrunA17_Chr8g0351881 47.692 65 34 0 299 363 316 380 2.50e-12 68.6
Msa1230560 MtrunA17_Chr1g0153241 40.449 89 45 2 277 365 70 150 3.08e-12 67.0
Msa1230560 MtrunA17_Chr3g0095391 38.144 97 56 2 268 364 281 373 1.14e-11 67.0
Msa1230560 MtrunA17_Chr3g0134741 53.125 64 29 1 300 363 230 292 1.50e-11 65.5
Msa1230560 MtrunA17_Chr7g0270871 32.895 152 88 5 216 364 126 266 4.85e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1230560 AT3G07340.1 42.773 512 183 18 1 480 1 434 6.62e-102 313
Msa1230560 AT3G07340.2 42.276 492 175 17 1 460 1 415 1.41e-92 289
Msa1230560 AT5G48560.1 42.166 517 196 19 25 506 50 498 1.07e-88 281
Msa1230560 AT5G48560.2 42.463 471 182 17 25 460 50 466 2.28e-80 258
Msa1230560 AT3G23690.1 45.522 268 103 10 221 479 113 346 5.71e-56 191
Msa1230560 AT1G68920.1 41.083 314 146 9 84 388 108 391 7.95e-53 186
Msa1230560 AT1G68920.4 41.083 314 146 9 84 388 108 391 7.95e-53 186
Msa1230560 AT1G68920.2 41.083 314 146 9 84 388 108 391 7.95e-53 186
Msa1230560 AT1G68920.3 41.640 317 139 11 84 388 108 390 1.29e-52 186
Msa1230560 AT1G26260.4 43.706 286 114 12 105 389 73 312 1.86e-48 172
Msa1230560 AT1G26260.1 43.706 286 114 12 105 389 73 312 1.86e-48 172
Msa1230560 AT1G26260.6 43.706 286 114 12 105 389 73 312 1.86e-48 172
Msa1230560 AT1G26260.2 43.706 286 114 12 105 389 73 312 1.86e-48 172
Msa1230560 AT1G59640.1 67.200 125 39 1 256 378 91 215 4.14e-48 167
Msa1230560 AT1G59640.2 67.200 125 39 1 256 378 91 215 2.36e-47 167
Msa1230560 AT1G26260.5 42.173 313 128 15 84 390 26 291 4.36e-46 165
Msa1230560 AT1G10120.2 52.258 155 59 3 251 391 144 297 6.09e-45 162
Msa1230560 AT1G10120.1 52.258 155 59 3 251 391 144 297 6.09e-45 162
Msa1230560 AT1G26260.3 63.768 138 40 4 262 390 126 262 6.30e-45 161
Msa1230560 AT5G50915.3 56.463 147 59 2 224 370 66 207 2.22e-43 155
Msa1230560 AT5G50915.4 56.463 147 59 2 224 370 66 207 2.22e-43 155
Msa1230560 AT5G50915.1 56.849 146 58 2 224 369 66 206 6.69e-43 154
Msa1230560 AT5G50915.2 56.849 146 58 2 224 369 66 206 6.69e-43 154
Msa1230560 AT2G18300.1 58.519 135 55 1 254 387 139 273 2.79e-42 154
Msa1230560 AT4G36540.2 51.351 185 74 5 204 387 60 229 3.88e-42 152
Msa1230560 AT4G36540.1 53.409 176 66 5 206 380 62 222 9.78e-42 151
Msa1230560 AT4G34530.1 78.409 88 19 0 293 380 166 253 1.02e-41 152
Msa1230560 AT2G18300.2 58.394 137 54 2 254 389 139 273 1.39e-41 152
Msa1230560 AT5G62610.2 76.087 92 17 1 280 371 139 225 1.47e-41 150
Msa1230560 AT5G62610.1 76.087 92 17 1 280 371 139 225 1.47e-41 150
Msa1230560 AT2G18300.3 58.394 137 54 2 254 389 139 273 2.58e-41 152
Msa1230560 AT2G42300.1 61.682 107 36 1 296 402 182 283 4.49e-38 142
Msa1230560 AT3G57800.2 64.078 103 32 1 296 398 201 298 4.58e-38 144
Msa1230560 AT2G42300.4 68.539 89 28 0 296 384 182 270 5.56e-38 140
Msa1230560 AT1G25330.1 67.677 99 29 2 294 391 99 195 5.48e-36 134
Msa1230560 AT1G73830.2 70.588 85 25 0 298 382 146 230 6.16e-34 129
Msa1230560 AT1G73830.1 70.588 85 25 0 298 382 146 230 6.25e-34 129
Msa1230560 AT1G18400.2 69.767 86 26 0 297 382 143 228 6.36e-34 129
Msa1230560 AT1G18400.1 69.767 86 26 0 297 382 143 228 8.69e-34 129
Msa1230560 AT3G57800.1 44.000 150 32 2 296 398 201 345 1.68e-29 120
Msa1230560 AT2G24260.2 65.753 73 24 1 292 364 28 99 4.68e-23 98.6
Msa1230560 AT2G24260.1 65.753 73 24 1 292 364 131 202 3.57e-22 98.2
Msa1230560 AT4G30980.1 72.727 66 17 1 299 364 130 194 4.82e-22 97.1
Msa1230560 AT4G30980.2 72.727 66 17 1 299 364 178 242 7.82e-22 97.4
Msa1230560 AT5G58010.1 56.667 90 38 1 275 364 75 163 1.03e-21 95.9
Msa1230560 AT2G42300.5 83.721 43 7 0 296 338 182 224 1.97e-18 84.7
Msa1230560 AT2G42300.3 83.721 43 7 0 296 338 182 224 1.97e-18 84.7
Msa1230560 AT2G42300.2 83.721 43 7 0 296 338 182 224 2.28e-18 84.7
Msa1230560 AT4G02590.3 42.336 137 68 4 228 364 22 147 2.10e-15 76.3
Msa1230560 AT2G20180.4 34.483 145 76 4 227 364 210 342 2.83e-15 78.6
Msa1230560 AT2G20180.6 34.483 145 76 4 227 364 210 342 2.83e-15 78.6
Msa1230560 AT2G20180.2 34.483 145 76 4 227 364 210 342 2.83e-15 78.6
Msa1230560 AT2G20180.3 34.483 145 76 4 227 364 210 342 2.83e-15 78.6
Msa1230560 AT2G20180.7 36.232 138 83 3 227 364 139 271 3.00e-15 78.2
Msa1230560 AT2G20180.1 36.232 138 83 3 227 364 139 271 3.00e-15 78.2
Msa1230560 AT2G20180.5 36.232 138 83 3 227 364 139 271 3.00e-15 78.2
Msa1230560 AT4G02590.2 42.336 137 68 4 228 364 85 210 3.05e-15 77.0
Msa1230560 AT4G02590.1 42.336 137 68 4 228 364 85 210 3.05e-15 77.0
Msa1230560 AT4G09180.1 52.239 67 32 0 297 363 182 248 1.99e-14 73.9
Msa1230560 AT2G42280.1 53.125 64 30 0 299 362 279 342 3.62e-14 74.3
Msa1230560 AT1G03040.1 50.476 105 41 4 282 376 124 227 4.52e-14 73.6
Msa1230560 AT1G51140.1 56.250 64 28 0 299 362 304 367 4.63e-14 74.3
Msa1230560 AT1G09530.3 39.583 96 47 2 269 364 317 401 5.20e-14 74.7
Msa1230560 AT1G09530.2 39.583 96 47 2 269 364 317 401 5.20e-14 74.7
Msa1230560 AT1G09530.5 39.583 96 47 2 269 364 317 401 5.20e-14 74.7
Msa1230560 AT1G09530.1 39.583 96 47 2 269 364 317 401 5.20e-14 74.7
Msa1230560 AT1G09530.4 39.583 96 47 2 269 364 317 401 5.20e-14 74.7
Msa1230560 AT1G09530.6 39.583 96 47 2 269 364 317 401 5.20e-14 74.7
Msa1230560 AT1G03040.2 50.000 106 42 4 281 376 118 222 6.49e-14 72.8
Msa1230560 AT2G42280.3 54.545 66 28 1 299 362 279 344 7.08e-14 73.6
Msa1230560 AT2G20180.8 35.556 135 82 3 227 361 210 339 7.88e-14 73.2
Msa1230560 AT1G35460.1 50.746 67 33 0 297 363 179 245 9.21e-14 72.0
Msa1230560 AT1G03040.3 50.476 105 41 4 282 376 124 227 9.35e-14 72.8
Msa1230560 AT4G28815.2 56.897 58 24 1 302 359 71 127 2.30e-13 70.1
Msa1230560 AT4G28800.1 35.099 151 71 6 213 363 189 312 4.10e-13 71.6
Msa1230560 AT4G28815.1 56.897 58 24 1 302 359 147 203 8.36e-13 69.7
Msa1230560 AT2G46970.1 34.884 129 67 4 241 364 171 287 1.03e-12 70.5
Msa1230560 AT2G46970.2 34.884 129 67 4 241 364 140 256 1.04e-12 70.1
Msa1230560 AT4G28800.2 34.161 161 78 7 213 372 201 334 1.18e-12 70.5
Msa1230560 AT2G46970.3 34.884 129 67 4 241 364 149 265 1.25e-12 70.1
Msa1230560 AT4G28790.1 50.000 64 31 1 301 364 273 335 6.96e-12 67.8
Msa1230560 AT4G33880.1 33.333 180 99 6 204 363 151 329 8.97e-12 67.0
Msa1230560 AT1G27740.1 43.678 87 48 1 277 363 145 230 1.11e-11 65.5
Msa1230560 AT2G14760.1 50.769 65 31 1 299 363 237 300 3.46e-11 65.1
Msa1230560 AT4G00050.2 50.769 65 31 1 299 363 105 168 3.93e-11 64.7
Msa1230560 AT2G43140.1 48.485 66 34 0 297 362 132 197 5.69e-11 62.8
Msa1230560 AT4G00050.1 50.769 65 31 1 299 363 207 270 7.97e-11 64.3
Msa1230560 AT4G00050.3 50.769 65 31 1 299 363 207 270 9.36e-11 63.9
Msa1230560 AT4G28790.2 45.455 66 35 1 301 366 273 337 9.68e-11 63.9

Find 126 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTCAGTTTCTATTCTTC+TGG 0.187268 8_2:+6592412 Msa1230560:three_prime_UTR
TCCGTTGACTTGTCCCAATT+TGG 0.196239 8_2:-6589120 None:intergenic
ATTATTCAATGTAAAATCTT+AGG 0.207531 8_2:+6592460 Msa1230560:three_prime_UTR
GAGGGAATTGATAGGAAAAT+TGG 0.208603 8_2:+6589248 Msa1230560:CDS
CTTTAGTGCTTTACCTTTGT+TGG 0.234392 8_2:-6589771 None:intergenic
ATGCATTTACCTTTCCTAAA+TGG 0.234846 8_2:+6591542 Msa1230560:CDS
AATTATACCTTGTAGGTTAC+TGG 0.243510 8_2:+6590996 Msa1230560:intron
TCTCATACTTTAATGGAAAA+TGG 0.244355 8_2:+6589495 Msa1230560:CDS
AATACACCAAATGGAGAAAT+TGG 0.248984 8_2:+6589669 Msa1230560:CDS
TTGAATAATTTGTAAAATAG+AGG 0.259110 8_2:-6592446 None:intergenic
CACCTTCATGAAATCCATTT+AGG 0.262102 8_2:-6591556 None:intergenic
TCCTCCTCAGAGAATGTTAA+TGG 0.266804 8_2:-6591957 None:intergenic
ATAGGAAAATTGGGAACTTT+TGG 0.267348 8_2:+6589258 Msa1230560:CDS
TCTTTGTGCCATAATAATAT+TGG 0.268551 8_2:+6591518 Msa1230560:CDS
CTGGGGTTGTTGCCCATAAA+AGG 0.272608 8_2:-6591423 None:intergenic
CAGTGGGTCCCATTTGTATT+AGG 0.280658 8_2:-6591473 None:intergenic
TCAAAGATCTCATACTTTAA+TGG 0.282745 8_2:+6589488 Msa1230560:CDS
GATCCTCTGGGCTTCAATTC+AGG 0.289630 8_2:-6592236 None:intergenic
TGCAAATTATTAGTGTTATA+TGG 0.294745 8_2:+6592503 Msa1230560:three_prime_UTR
TGAAAATGGTATCAAGAATA+TGG 0.300257 8_2:+6590141 Msa1230560:CDS
ACCGTTGTTCAGATGGGATT+TGG 0.307096 8_2:+6591987 Msa1230560:CDS
AATGCATGCCAATATTATTA+TGG 0.315999 8_2:-6591526 None:intergenic
TAATTAAGCCATTATTGATT+AGG 0.316196 8_2:+6592311 Msa1230560:three_prime_UTR
TTGGACATGTTGTTGGGATT+TGG 0.322517 8_2:-6589210 None:intergenic
ATTATAATTCATGGGGTTGT+TGG 0.331342 8_2:-6592292 None:intergenic
AATGGCTTAATTATAATTCA+TGG 0.340189 8_2:-6592301 None:intergenic
TGTGGCAAAGAGAGACTAAT+TGG 0.341743 8_2:-6588831 None:intergenic
CTTAAATGCAAAGAAAATTA+AGG 0.345742 8_2:+6590099 Msa1230560:CDS
CTGAATCAAAGTTAAGGTTT+TGG 0.361916 8_2:-6589143 None:intergenic
CCGTTGACTTGTCCCAATTT+GGG 0.371481 8_2:-6589119 None:intergenic
TGCAGCCTGGTACAAGATCT+TGG 0.374431 8_2:-6590821 None:intergenic
TTGACACCAATTTCTCCATT+TGG 0.376211 8_2:-6589675 None:intergenic
ATTTCAGTTTCTATTCTTCT+GGG 0.376480 8_2:+6592413 Msa1230560:three_prime_UTR
ACAACATTCTTCTAACCCTT+TGG 0.378957 8_2:+6589290 Msa1230560:CDS
TTGATAAAATGGTTCATCAT+TGG 0.379781 8_2:-6589318 None:intergenic
AATAACATTCCTAATACAAA+TGG 0.381646 8_2:+6591464 Msa1230560:CDS
CAACATTGTTCATCACCATT+TGG 0.383931 8_2:-6589458 None:intergenic
GTGCTTTACCTTTGTTGGAT+TGG 0.388636 8_2:-6589766 None:intergenic
TGAACAGACCTAATCAATAA+TGG 0.400480 8_2:-6592319 None:intergenic
GTGTATTCCTGTTTGAAGTT+TGG 0.400671 8_2:-6592010 None:intergenic
TTTGTTGGATTGGAAGAGTT+TGG 0.402500 8_2:-6589756 None:intergenic
GAGGGTGAGAGAAATGAAAA+TGG 0.403613 8_2:+6590127 Msa1230560:CDS
AGTCCATCACTCAAAACATT+TGG 0.415104 8_2:+6589543 Msa1230560:CDS
GAATTGAAGCCCAGAGGATC+AGG 0.415764 8_2:+6592239 Msa1230560:three_prime_UTR
TCTTGTACCAGGCTGCAATA+AGG 0.425820 8_2:+6590827 Msa1230560:CDS
ATGGTACTGAGGAATCTAAT+TGG 0.426318 8_2:-6591400 None:intergenic
AGAAAAGGGAAAGGGAGGGA+AGG 0.428185 8_2:-6588741 None:intergenic
AAGAGAGAAAAGGGAAAGGG+AGG 0.432099 8_2:-6588746 None:intergenic
AGGATTGATTCTGTATTGTA+TGG 0.441963 8_2:+6592480 Msa1230560:three_prime_UTR
AGGGAATTGATAGGAAAATT+GGG 0.442979 8_2:+6589249 Msa1230560:CDS
CTTCACATGATATGAATTCA+AGG 0.443498 8_2:+6589700 Msa1230560:CDS
ATGGCTTAATTATAATTCAT+GGG 0.454114 8_2:-6592300 None:intergenic
ATGGAGAATTCTTGTTCTCA+TGG 0.459183 8_2:-6589581 None:intergenic
TTTGAGGAAAAGAGAGAAAA+GGG 0.459233 8_2:-6588755 None:intergenic
TGGGTCTATTTAACCCATGA+TGG 0.460226 8_2:+6588706 None:intergenic
TCTGAGCAAAATACACCAAA+TGG 0.461659 8_2:+6589660 Msa1230560:CDS
GAATGTTATTATGAATAGCT+TGG 0.462285 8_2:-6591451 None:intergenic
ATTTCATGAAGGTGGTTCTC+AGG 0.463838 8_2:+6591565 Msa1230560:CDS
CTTTGAGGAAAAGAGAGAAA+AGG 0.466312 8_2:-6588756 None:intergenic
GACATGTTGTTGGGATTTGG+TGG 0.473535 8_2:-6589207 None:intergenic
TTCCTAAATGGATTTCATGA+AGG 0.474350 8_2:+6591554 Msa1230560:CDS
TTGATTGTAAATAGGTTAGG+AGG 0.479419 8_2:+6590769 Msa1230560:intron
CTTAAACACCGTTGTTCAGA+TGG 0.482331 8_2:+6591980 Msa1230560:CDS
TTGAGAGGAAAGACTCTTTG+AGG 0.485023 8_2:-6588771 None:intergenic
GGTGGTGATGATGTAGCTGC+TGG 0.485718 8_2:-6589189 None:intergenic
GATTGAAATTGAGGCTTGAG+AGG 0.487579 8_2:-6588786 None:intergenic
CCACTGCTCAGTGGAGCCAT+TGG 0.490185 8_2:+6591490 Msa1230560:CDS
GAAAAGAGAGAAAAGGGAAA+GGG 0.492222 8_2:-6588749 None:intergenic
AGAGAGAAAAGGGAAAGGGA+GGG 0.493215 8_2:-6588745 None:intergenic
TTGGGGTTGGATTGAAATTG+AGG 0.494067 8_2:-6588795 None:intergenic
GATTTGGCCAAACTTCAAAC+AGG 0.499864 8_2:+6592003 Msa1230560:CDS
CTTTAGGGTGTTGAAGGTAG+TGG 0.502252 8_2:+6590070 Msa1230560:intron
TTGAAACTTGGGGAGTGTAT+TGG 0.502535 8_2:-6592032 None:intergenic
TAAGTGCTGAATCAAAGTTA+AGG 0.502693 8_2:-6589149 None:intergenic
GTCTTCAGTGAACACAAAGA+TGG 0.504687 8_2:+6591186 Msa1230560:CDS
AAAGAAAATTAAGGCAAATG+AGG 0.506222 8_2:+6590108 Msa1230560:CDS
GCAACAAGAAAATGATAGAA+TGG 0.507282 8_2:+6589605 Msa1230560:CDS
CTTTGAACAAAACCAGGATC+AGG 0.511674 8_2:-6589384 None:intergenic
GTCATTGCAGCGTCAAGTTG+AGG 0.516912 8_2:+6591058 Msa1230560:CDS
CTTCTCCAAGATCTTGTACC+AGG 0.517031 8_2:+6590816 Msa1230560:CDS
TGAATAATTTGTAAAATAGA+GGG 0.519597 8_2:-6592445 None:intergenic
TTGTTTACCTTATTGCAGCC+TGG 0.519916 8_2:-6590834 None:intergenic
AGAAAATGATAGAATGGAAG+TGG 0.520012 8_2:+6589611 Msa1230560:CDS
ATAACATTCCTAATACAAAT+GGG 0.521184 8_2:+6591465 Msa1230560:CDS
TTAATTTAGCAATGATACAT+TGG 0.522229 8_2:-6592528 None:intergenic
TTCATCATTGGTACAACCAA+AGG 0.522984 8_2:-6589306 None:intergenic
CTAAATGGATTTCATGAAGG+TGG 0.524147 8_2:+6591557 Msa1230560:CDS
TGATTGTAAATAGGTTAGGA+GGG 0.531230 8_2:+6590770 Msa1230560:intron
AAGAGAATTCAAAGAAGTAG+TGG 0.535245 8_2:-6588857 None:intergenic
AGAAAAGCTTCTTCTTCCAA+AGG 0.537859 8_2:+6589726 Msa1230560:CDS
GGAAAAGAGAGAAAAGGGAA+AGG 0.539564 8_2:-6588750 None:intergenic
AAATCTGCCACATTAGACAT+TGG 0.540075 8_2:-6589357 None:intergenic
TCATCATTGGTACAACCAAA+GGG 0.546397 8_2:-6589305 None:intergenic
TGGAGGAAGAGACCAAAGAA+GGG 0.551819 8_2:+6590161 Msa1230560:CDS
CAATGAGAGAGGTAACCAAA+TGG 0.556438 8_2:+6589443 Msa1230560:CDS
GTGGTGATGATGTAGCTGCT+GGG 0.558812 8_2:-6589188 None:intergenic
AAGAAAATTAAGGCAAATGA+GGG 0.561150 8_2:+6590109 Msa1230560:CDS
CCCAAATTGGGACAAGTCAA+CGG 0.562105 8_2:+6589119 Msa1230560:CDS
GCAGCCCTTTGAACAAAACC+AGG 0.562440 8_2:-6589390 None:intergenic
GGAGGGAGAAAATAAGTGAG+AGG 0.565662 8_2:+6590787 Msa1230560:CDS
TTCAATTCAGGCGTAATGAA+AGG 0.571791 8_2:-6592224 None:intergenic
CAATGGCTCCACTGAGCAGT+GGG 0.574239 8_2:-6591489 None:intergenic
TTTGTGATGAGGGAATTGAT+AGG 0.574585 8_2:+6589240 Msa1230560:CDS
TCATGAAATCCATTTAGGAA+AGG 0.586062 8_2:-6591551 None:intergenic
TGGCTTAATTATAATTCATG+GGG 0.586205 8_2:-6592299 None:intergenic
ATGGAGGAAGAGACCAAAGA+AGG 0.588900 8_2:+6590160 Msa1230560:CDS
TTTATTACCGTTGAAACTTG+GGG 0.589614 8_2:-6592042 None:intergenic
TTAAACACCGTTGTTCAGAT+GGG 0.591278 8_2:+6591981 Msa1230560:CDS
AGCAAGAGTTTCAATGAGAG+AGG 0.594170 8_2:+6589432 Msa1230560:CDS
TCCATTAACATTCTCTGAGG+AGG 0.599758 8_2:+6591956 Msa1230560:CDS
CCAATGGCTCCACTGAGCAG+TGG 0.605881 8_2:-6591490 None:intergenic
GGAAGTGGCTAATTCTCAAG+AGG 0.609764 8_2:+6589626 Msa1230560:CDS
ATACACTCCCCAAGTTTCAA+CGG 0.637097 8_2:+6592035 Msa1230560:CDS
TGGTGATGATGTAGCTGCTG+GGG 0.642200 8_2:-6589187 None:intergenic
AAATGGTATCAAGAATATGG+AGG 0.642262 8_2:+6590144 Msa1230560:CDS
ATTCATGTCAGAGCAAGAAG+AGG 0.647303 8_2:+6590238 Msa1230560:CDS
TGGCACAAAGAAGAATCCAA+TGG 0.648457 8_2:-6591506 None:intergenic
GGTTGTTGCCCATAAAAGGA+TGG 0.650244 8_2:-6591419 None:intergenic
CCCATAAAAGGATGGTACTG+AGG 0.650891 8_2:-6591411 None:intergenic
GCCAAATCCCATCTGAACAA+CGG 0.655409 8_2:-6591988 None:intergenic
TCAAAGAAGTAGTGGCAATG+TGG 0.656678 8_2:-6588849 None:intergenic
AAACTCTTCCAATCCAACAA+AGG 0.664009 8_2:+6589758 Msa1230560:CDS
AAATGGGACCCACTGCTCAG+TGG 0.666661 8_2:+6591481 Msa1230560:CDS
GGAGGAAGAGACCAAAGAAG+GGG 0.668214 8_2:+6590162 Msa1230560:CDS
GTATCCATTAACATTCTCTG+AGG 0.704907 8_2:+6591953 Msa1230560:intron
ACGCCTGAATTGAAGCCCAG+AGG 0.732491 8_2:+6592233 Msa1230560:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTGTTTAAATTTTTGA+CGG - chr8_2:6589871-6589890 None:intergenic 10.0%
!!! CAATTTTTTTTTTTTTATGT+TGG + chr8_2:6590020-6590039 Msa1230560:intron 10.0%
!!! ATAATTTTATGTAATATCAA+AGG - chr8_2:6590372-6590391 None:intergenic 10.0%
!! TTTGATATTACATAAAATTA+TGG + chr8_2:6590371-6590390 Msa1230560:intron 10.0%
!!! TTTTGTTATGTTTTAAATTA+AGG - chr8_2:6590701-6590720 None:intergenic 10.0%
!!! TTTTTTTTTTTTTGAAAGAA+AGG + chr8_2:6590860-6590879 Msa1230560:intron 10.0%
!!! AAATAAGTTTTGATTAATTA+AGG - chr8_2:6592149-6592168 None:intergenic 10.0%
!!! AATTAATCAAAACTTATTTT+TGG + chr8_2:6592150-6592169 Msa1230560:intron 10.0%
!! TAAAAAAGAATTTAGATCAA+AGG - chr8_2:6589027-6589046 None:intergenic 15.0%
!!! ATATTTTAGTCAAAATAAAC+CGG - chr8_2:6589922-6589941 None:intergenic 15.0%
!! AACAAAAAAAAAATTCGATA+GGG - chr8_2:6590321-6590340 None:intergenic 15.0%
!!! TTTTTTTTTTGTTGTGAAAT+TGG + chr8_2:6590328-6590347 Msa1230560:intron 15.0%
!! AGGTATAATTAATAATTCAT+AGG - chr8_2:6590986-6591005 None:intergenic 15.0%
!! AATTATTAATTATACCTTGT+AGG + chr8_2:6590989-6591008 Msa1230560:intron 15.0%
!!! ATTAAAATGTCATTTTCATT+TGG + chr8_2:6592109-6592128 Msa1230560:intron 15.0%
!!! TGAATAATTTGTAAAATAGA+GGG - chr8_2:6592448-6592467 None:intergenic 15.0%
!!! TTGAATAATTTGTAAAATAG+AGG - chr8_2:6592449-6592468 None:intergenic 15.0%
!! ATTATTCAATGTAAAATCTT+AGG + chr8_2:6592460-6592479 Msa1230560:three_prime_UTR 15.0%
!!! ACAAAATGAACTTAATTTTG+AGG - chr8_2:6588982-6589001 None:intergenic 20.0%
!!! AATTCTTTTTTAGTCCATTA+TGG + chr8_2:6589035-6589054 Msa1230560:exon 20.0%
!!! GCATATTTTCTTTGATAAAA+TGG - chr8_2:6589332-6589351 None:intergenic 20.0%
!! CTTAAATGCAAAGAAAATTA+AGG + chr8_2:6590099-6590118 Msa1230560:CDS 20.0%
!! CAACAAAAAAAAAATTCGAT+AGG - chr8_2:6590322-6590341 None:intergenic 20.0%
!! ATGAATCTTTGATTGTAAAT+AGG + chr8_2:6590761-6590780 Msa1230560:intron 20.0%
!!! TTTGTACTTTTTCACTAATT+TGG + chr8_2:6590955-6590974 Msa1230560:intron 20.0%
!! TATAGAATTAAGATGCTTAT+TGG - chr8_2:6591098-6591117 None:intergenic 20.0%
!!! AAATATCTGAAATCACAAAA+AGG - chr8_2:6591348-6591367 None:intergenic 20.0%
!! AATAACATTCCTAATACAAA+TGG + chr8_2:6591464-6591483 Msa1230560:CDS 20.0%
!! ATAACATTCCTAATACAAAT+GGG + chr8_2:6591465-6591484 Msa1230560:CDS 20.0%
!! CTTCATATATTAGTACATAT+GGG - chr8_2:6591870-6591889 None:intergenic 20.0%
!! ATTAATTAAGGTCAAATTTC+TGG - chr8_2:6592137-6592156 None:intergenic 20.0%
!! ATGGCTTAATTATAATTCAT+GGG - chr8_2:6592303-6592322 None:intergenic 20.0%
!! AATGGCTTAATTATAATTCA+TGG - chr8_2:6592304-6592323 None:intergenic 20.0%
!!! TAATTAAGCCATTATTGATT+AGG + chr8_2:6592311-6592330 Msa1230560:three_prime_UTR 20.0%
!! CTAACTTAAAAAACTAACAA+AGG - chr8_2:6592355-6592374 None:intergenic 20.0%
!! TGCAAATTATTAGTGTTATA+TGG + chr8_2:6592503-6592522 Msa1230560:three_prime_UTR 20.0%
!! TTAATTTAGCAATGATACAT+TGG - chr8_2:6592531-6592550 None:intergenic 20.0%
!!! ATTGGAGATTTTGATGAATT+GGG - chr8_2:6588816-6588835 None:intergenic 25.0%
!!! AATTGGAGATTTTGATGAAT+TGG - chr8_2:6588817-6588836 None:intergenic 25.0%
!!! AAAGAATTGGTTTTCCATAA+TGG - chr8_2:6589052-6589071 None:intergenic 25.0%
!! AACCAATTCTTTTTAAATGC+TGG + chr8_2:6589060-6589079 Msa1230560:CDS 25.0%
! ATCCAGCATTTAAAAAGAAT+TGG - chr8_2:6589065-6589084 None:intergenic 25.0%
!!! ATTGTTTTTTCAACCCAAAT+TGG + chr8_2:6589106-6589125 Msa1230560:CDS 25.0%
!!! TTGTTTTTTCAACCCAAATT+GGG + chr8_2:6589107-6589126 Msa1230560:CDS 25.0%
!! CAATGAAAATTTTGTGATGA+GGG + chr8_2:6589230-6589249 Msa1230560:CDS 25.0%
! TTGATAAAATGGTTCATCAT+TGG - chr8_2:6589321-6589340 None:intergenic 25.0%
! TCAAAGATCTCATACTTTAA+TGG + chr8_2:6589488-6589507 Msa1230560:CDS 25.0%
! TCTCATACTTTAATGGAAAA+TGG + chr8_2:6589495-6589514 Msa1230560:CDS 25.0%
!! GATGACAAAAAGTCTAAAAT+GGG - chr8_2:6589820-6589839 None:intergenic 25.0%
!!! AAATTTTAGTCCATAGAGTA+TGG + chr8_2:6589885-6589904 Msa1230560:intron 25.0%
!!! TTGAGTGTTACAATTTTTTC+CGG + chr8_2:6589962-6589981 Msa1230560:intron 25.0%
!!! TAAAAACATATTTTAGTCCC+CGG - chr8_2:6589984-6590003 None:intergenic 25.0%
!!! ATCTCATTTCTTTTGACTTT+AGG + chr8_2:6590054-6590073 Msa1230560:intron 25.0%
!!! TCTCATTTCTTTTGACTTTA+GGG + chr8_2:6590055-6590074 Msa1230560:intron 25.0%
! AAAGAAAATTAAGGCAAATG+AGG + chr8_2:6590108-6590127 Msa1230560:CDS 25.0%
! AAGAAAATTAAGGCAAATGA+GGG + chr8_2:6590109-6590128 Msa1230560:CDS 25.0%
! TGAAAATGGTATCAAGAATA+TGG + chr8_2:6590141-6590160 Msa1230560:CDS 25.0%
!! ACATGAATGTAATCTTTTTG+AGG - chr8_2:6590226-6590245 None:intergenic 25.0%
! TTCCAAAAACATGATATTGT+GGG + chr8_2:6590649-6590668 Msa1230560:intron 25.0%
! TCTTTGATTGTAAATAGGTT+AGG + chr8_2:6590766-6590785 Msa1230560:intron 25.0%
! AACCAGAATACAAAACAAAT+TGG + chr8_2:6590908-6590927 Msa1230560:intron 25.0%
!!! AACCAATTTGTTTTGTATTC+TGG - chr8_2:6590913-6590932 None:intergenic 25.0%
!! TTTTCTTGCTCATTAAGTTA+TGG + chr8_2:6591133-6591152 Msa1230560:intron 25.0%
! GAATGTTATTATGAATAGCT+TGG - chr8_2:6591454-6591473 None:intergenic 25.0%
! TCTTTGTGCCATAATAATAT+TGG + chr8_2:6591518-6591537 Msa1230560:CDS 25.0%
! AATGCATGCCAATATTATTA+TGG - chr8_2:6591529-6591548 None:intergenic 25.0%
! CAGTTAAAAAGTTCATTAAC+CGG - chr8_2:6591741-6591760 None:intergenic 25.0%
! GTCTTAATTATCTGATTCAT+AGG - chr8_2:6591790-6591809 None:intergenic 25.0%
! CCTTCATATATTAGTACATA+TGG - chr8_2:6591871-6591890 None:intergenic 25.0%
! CCATATGTACTAATATATGA+AGG + chr8_2:6591868-6591887 Msa1230560:intron 25.0%
!! TTTTATTACCGTTGAAACTT+GGG - chr8_2:6592046-6592065 None:intergenic 25.0%
! TGGCTTAATTATAATTCATG+GGG - chr8_2:6592302-6592321 None:intergenic 25.0%
! TATTTCAGTTTCTATTCTTC+TGG + chr8_2:6592412-6592431 Msa1230560:three_prime_UTR 25.0%
! ATTTCAGTTTCTATTCTTCT+GGG + chr8_2:6592413-6592432 Msa1230560:three_prime_UTR 25.0%
TTTGAGGAAAAGAGAGAAAA+GGG - chr8_2:6588758-6588777 None:intergenic 30.0%
!! TTGGAGATTTTGATGAATTG+GGG - chr8_2:6588815-6588834 None:intergenic 30.0%
AAGAGAATTCAAAGAAGTAG+TGG - chr8_2:6588860-6588879 None:intergenic 30.0%
CCTTGAAACTAGAAGAAAAA+TGG - chr8_2:6588912-6588931 None:intergenic 30.0%
!!! CCATTTTTCTTCTAGTTTCA+AGG + chr8_2:6588909-6588928 Msa1230560:five_prime_UTR 30.0%
!! AGTTTCTTTTGCTTTTGGTT+TGG - chr8_2:6588958-6588977 None:intergenic 30.0%
TGAAAAAACAATCTTGGGTT+TGG - chr8_2:6589098-6589117 None:intergenic 30.0%
! TGGGTTGAAAAAACAATCTT+GGG - chr8_2:6589103-6589122 None:intergenic 30.0%
!! TTGGGTTGAAAAAACAATCT+TGG - chr8_2:6589104-6589123 None:intergenic 30.0%
! CTGAATCAAAGTTAAGGTTT+TGG - chr8_2:6589146-6589165 None:intergenic 30.0%
!! TAAGTGCTGAATCAAAGTTA+AGG - chr8_2:6589152-6589171 None:intergenic 30.0%
! TTTTCATTGGACATGTTGTT+GGG - chr8_2:6589219-6589238 None:intergenic 30.0%
! ATTTTCATTGGACATGTTGT+TGG - chr8_2:6589220-6589239 None:intergenic 30.0%
! CCTCATCACAAAATTTTCAT+TGG - chr8_2:6589232-6589251 None:intergenic 30.0%
! CCAATGAAAATTTTGTGATG+AGG + chr8_2:6589229-6589248 Msa1230560:CDS 30.0%
AGGGAATTGATAGGAAAATT+GGG + chr8_2:6589249-6589268 Msa1230560:CDS 30.0%
! ATAGGAAAATTGGGAACTTT+TGG + chr8_2:6589258-6589277 Msa1230560:CDS 30.0%
! CTATCATTTTCTTGTTGCAA+TGG - chr8_2:6589603-6589622 None:intergenic 30.0%
GCAACAAGAAAATGATAGAA+TGG + chr8_2:6589605-6589624 Msa1230560:CDS 30.0%
AGAAAATGATAGAATGGAAG+TGG + chr8_2:6589611-6589630 Msa1230560:CDS 30.0%
AATACACCAAATGGAGAAAT+TGG + chr8_2:6589669-6589688 Msa1230560:CDS 30.0%
CTTCACATGATATGAATTCA+AGG + chr8_2:6589700-6589719 Msa1230560:CDS 30.0%
CGATGACAAAAAGTCTAAAA+TGG - chr8_2:6589821-6589840 None:intergenic 30.0%
ACAATTTAGTCCATACTCTA+TGG - chr8_2:6589898-6589917 None:intergenic 30.0%
!!! TGAGTGTTACAATTTTTTCC+GGG + chr8_2:6589963-6589982 Msa1230560:intron 30.0%
AAATGGTATCAAGAATATGG+AGG + chr8_2:6590144-6590163 Msa1230560:CDS 30.0%
!!! ATGTAATCTTTTTGAGGTTC+AGG - chr8_2:6590220-6590239 None:intergenic 30.0%
TTCAACAACCACTAATATGT+TGG - chr8_2:6590477-6590496 None:intergenic 30.0%
TTGCACTGTAATCATATCAA+TGG - chr8_2:6590590-6590609 None:intergenic 30.0%
CTTCCAAAAACATGATATTG+TGG + chr8_2:6590648-6590667 Msa1230560:intron 30.0%
TTAAGGTTTGCAACTACTAT+TGG - chr8_2:6590684-6590703 None:intergenic 30.0%
TTGATTGTAAATAGGTTAGG+AGG + chr8_2:6590769-6590788 Msa1230560:intron 30.0%
TGATTGTAAATAGGTTAGGA+GGG + chr8_2:6590770-6590789 Msa1230560:intron 30.0%
AATTATACCTTGTAGGTTAC+TGG + chr8_2:6590996-6591015 Msa1230560:intron 30.0%
ATGCATTTACCTTTCCTAAA+TGG + chr8_2:6591542-6591561 Msa1230560:CDS 30.0%
TCATGAAATCCATTTAGGAA+AGG - chr8_2:6591554-6591573 None:intergenic 30.0%
! TTCCTAAATGGATTTCATGA+AGG + chr8_2:6591554-6591573 Msa1230560:CDS 30.0%
TAACTGAATTTCAAACCATC+AGG + chr8_2:6591755-6591774 Msa1230560:intron 30.0%
TTAGTACATATGGGACAATT+GGG - chr8_2:6591861-6591880 None:intergenic 30.0%
ATTAGTACATATGGGACAAT+TGG - chr8_2:6591862-6591881 None:intergenic 30.0%
TTTATTACCGTTGAAACTTG+GGG - chr8_2:6592045-6592064 None:intergenic 30.0%
!! GTTTTATTACCGTTGAAACT+TGG - chr8_2:6592047-6592066 None:intergenic 30.0%
ATTATAATTCATGGGGTTGT+TGG - chr8_2:6592295-6592314 None:intergenic 30.0%
TGAACAGACCTAATCAATAA+TGG - chr8_2:6592322-6592341 None:intergenic 30.0%
! AGGATTGATTCTGTATTGTA+TGG + chr8_2:6592480-6592499 Msa1230560:three_prime_UTR 30.0%
GAAAAAGATTAGGCCATCAT+GGG - chr8_2:6588722-6588741 None:intergenic 35.0%
GAAAAGAGAGAAAAGGGAAA+GGG - chr8_2:6588752-6588771 None:intergenic 35.0%
! CTTTGAGGAAAAGAGAGAAA+AGG - chr8_2:6588759-6588778 None:intergenic 35.0%
!! AGATTTTGATGAATTGGGGT+TGG - chr8_2:6588811-6588830 None:intergenic 35.0%
!!! CTTTTGCTTTTGGTTTGGTT+TGG - chr8_2:6588953-6588972 None:intergenic 35.0%
! GAGGTAGTTTCTTTTGCTTT+TGG - chr8_2:6588963-6588982 None:intergenic 35.0%
TTTGTGATGAGGGAATTGAT+AGG + chr8_2:6589240-6589259 Msa1230560:CDS 35.0%
GAGGGAATTGATAGGAAAAT+TGG + chr8_2:6589248-6589267 Msa1230560:CDS 35.0%
ACAACATTCTTCTAACCCTT+TGG + chr8_2:6589290-6589309 Msa1230560:CDS 35.0%
TCATCATTGGTACAACCAAA+GGG - chr8_2:6589308-6589327 None:intergenic 35.0%
TTCATCATTGGTACAACCAA+AGG - chr8_2:6589309-6589328 None:intergenic 35.0%
GCAAAAACCAATGTCTAATG+TGG + chr8_2:6589350-6589369 Msa1230560:CDS 35.0%
AAATCTGCCACATTAGACAT+TGG - chr8_2:6589360-6589379 None:intergenic 35.0%
!!! TGATCCTGGTTTTGTTCAAA+GGG + chr8_2:6589386-6589405 Msa1230560:CDS 35.0%
! GCTGCAAAATTTTCTTGCTT+TGG + chr8_2:6589408-6589427 Msa1230560:CDS 35.0%
CAACATTGTTCATCACCATT+TGG - chr8_2:6589461-6589480 None:intergenic 35.0%
AGTCCATCACTCAAAACATT+TGG + chr8_2:6589543-6589562 Msa1230560:CDS 35.0%
!! GATCCAAATGTTTTGAGTGA+TGG - chr8_2:6589549-6589568 None:intergenic 35.0%
ATGGAGAATTCTTGTTCTCA+TGG - chr8_2:6589584-6589603 None:intergenic 35.0%
TCTGAGCAAAATACACCAAA+TGG + chr8_2:6589660-6589679 Msa1230560:CDS 35.0%
TTGACACCAATTTCTCCATT+TGG - chr8_2:6589678-6589697 None:intergenic 35.0%
AGAAAAGCTTCTTCTTCCAA+AGG + chr8_2:6589726-6589745 Msa1230560:CDS 35.0%
! TTTGTTGGATTGGAAGAGTT+TGG - chr8_2:6589759-6589778 None:intergenic 35.0%
AAACTCTTCCAATCCAACAA+AGG + chr8_2:6589758-6589777 Msa1230560:CDS 35.0%
CTTTAGTGCTTTACCTTTGT+TGG - chr8_2:6589774-6589793 None:intergenic 35.0%
!!! GCTGCAATTTTTTAAGAGTG+CGG + chr8_2:6589793-6589812 Msa1230560:intron 35.0%
! GAGTATGGACTAAATTGTTC+CGG + chr8_2:6589900-6589919 Msa1230560:intron 35.0%
!!! GAGTGTTACAATTTTTTCCG+GGG + chr8_2:6589964-6589983 Msa1230560:intron 35.0%
!!! TTTTGACTTTAGGGTGTTGA+AGG + chr8_2:6590064-6590083 Msa1230560:intron 35.0%
!!! TAATCTTTTTGAGGTTCAGG+AGG - chr8_2:6590217-6590236 None:intergenic 35.0%
GCAACTACTATTGGATATTG+TGG - chr8_2:6590675-6590694 None:intergenic 35.0%
! ATGGTACTGAGGAATCTAAT+TGG - chr8_2:6591403-6591422 None:intergenic 35.0%
!!! ATGAATAGCTTGGTTTTGCT+GGG - chr8_2:6591444-6591463 None:intergenic 35.0%
!!! TATGAATAGCTTGGTTTTGC+TGG - chr8_2:6591445-6591464 None:intergenic 35.0%
CACCTTCATGAAATCCATTT+AGG - chr8_2:6591559-6591578 None:intergenic 35.0%
! CTAAATGGATTTCATGAAGG+TGG + chr8_2:6591557-6591576 Msa1230560:CDS 35.0%
ATCAAGTGATTGAGTTCTAC+CGG + chr8_2:6591719-6591738 Msa1230560:intron 35.0%
GTATCCATTAACATTCTCTG+AGG + chr8_2:6591953-6591972 Msa1230560:intron 35.0%
TTAAACACCGTTGTTCAGAT+GGG + chr8_2:6591981-6592000 Msa1230560:CDS 35.0%
! GTGTATTCCTGTTTGAAGTT+TGG - chr8_2:6592013-6592032 None:intergenic 35.0%
!!! ACTTATTTTTGGTCCAATGC+AGG + chr8_2:6592161-6592180 Msa1230560:intron 35.0%
!! TGATTGATTTGAACCTGCAT+TGG - chr8_2:6592177-6592196 None:intergenic 35.0%
TTCAATTCAGGCGTAATGAA+AGG - chr8_2:6592227-6592246 None:intergenic 35.0%
GGAAAAAGATTAGGCCATCA+TGG - chr8_2:6588723-6588742 None:intergenic 40.0%
GGAAAAGAGAGAAAAGGGAA+AGG - chr8_2:6588753-6588772 None:intergenic 40.0%
TTGAGAGGAAAGACTCTTTG+AGG - chr8_2:6588774-6588793 None:intergenic 40.0%
GATTGAAATTGAGGCTTGAG+AGG - chr8_2:6588789-6588808 None:intergenic 40.0%
TTGGGGTTGGATTGAAATTG+AGG - chr8_2:6588798-6588817 None:intergenic 40.0%
TGTGGCAAAGAGAGACTAAT+TGG - chr8_2:6588834-6588853 None:intergenic 40.0%
TCAAAGAAGTAGTGGCAATG+TGG - chr8_2:6588852-6588871 None:intergenic 40.0%
TTGGACATGTTGTTGGGATT+TGG - chr8_2:6589213-6589232 None:intergenic 40.0%
CTTTGAACAAAACCAGGATC+AGG - chr8_2:6589387-6589406 None:intergenic 40.0%
!!! CTGATCCTGGTTTTGTTCAA+AGG + chr8_2:6589385-6589404 Msa1230560:CDS 40.0%
AGCAAGAGTTTCAATGAGAG+AGG + chr8_2:6589432-6589451 Msa1230560:CDS 40.0%
CAATGAGAGAGGTAACCAAA+TGG + chr8_2:6589443-6589462 Msa1230560:CDS 40.0%
!!! AGAGTTTGGTGCTTTTCCTT+TGG - chr8_2:6589745-6589764 None:intergenic 40.0%
GTGCTTTACCTTTGTTGGAT+TGG - chr8_2:6589769-6589788 None:intergenic 40.0%
GAGGGTGAGAGAAATGAAAA+TGG + chr8_2:6590127-6590146 Msa1230560:CDS 40.0%
!! CTGTTTTTCTTCCCCTTCTT+TGG - chr8_2:6590176-6590195 None:intergenic 40.0%
ATTCATGTCAGAGCAAGAAG+AGG + chr8_2:6590238-6590257 Msa1230560:CDS 40.0%
TCATGCAGCCAACATATTAG+TGG + chr8_2:6590466-6590485 Msa1230560:intron 40.0%
AGTGGTTGTTGAATCGAGAT+CGG + chr8_2:6590484-6590503 Msa1230560:intron 40.0%
ACAGTGCAAACGCGTTAAAT+CGG + chr8_2:6590601-6590620 Msa1230560:intron 40.0%
! GGCCCACAATATCATGTTTT+TGG - chr8_2:6590654-6590673 None:intergenic 40.0%
TTGTTTACCTTATTGCAGCC+TGG - chr8_2:6590837-6590856 None:intergenic 40.0%
! TGCTTTTCCAGTAACCTACA+AGG - chr8_2:6591006-6591025 None:intergenic 40.0%
GTCTTCAGTGAACACAAAGA+TGG + chr8_2:6591186-6591205 Msa1230560:CDS 40.0%
! TCCTCAGTACCATCCTTTTA+TGG + chr8_2:6591410-6591429 Msa1230560:CDS 40.0%
! CCTCAGTACCATCCTTTTAT+GGG + chr8_2:6591411-6591430 Msa1230560:CDS 40.0%
!!! TGAATAGCTTGGTTTTGCTG+GGG - chr8_2:6591443-6591462 None:intergenic 40.0%
TGGCACAAAGAAGAATCCAA+TGG - chr8_2:6591509-6591528 None:intergenic 40.0%
ATTTCATGAAGGTGGTTCTC+AGG + chr8_2:6591565-6591584 Msa1230560:CDS 40.0%
! TCCATTAACATTCTCTGAGG+AGG + chr8_2:6591956-6591975 Msa1230560:CDS 40.0%
TCCTCCTCAGAGAATGTTAA+TGG - chr8_2:6591960-6591979 None:intergenic 40.0%
CTTAAACACCGTTGTTCAGA+TGG + chr8_2:6591980-6591999 Msa1230560:CDS 40.0%
GATTTGGCCAAACTTCAAAC+AGG + chr8_2:6592003-6592022 Msa1230560:CDS 40.0%
! TTGAAACTTGGGGAGTGTAT+TGG - chr8_2:6592035-6592054 None:intergenic 40.0%
ATACACTCCCCAAGTTTCAA+CGG + chr8_2:6592035-6592054 Msa1230560:CDS 40.0%
GGGAGGGAAGGAAAAAGATT+AGG - chr8_2:6588732-6588751 None:intergenic 45.0%
AGAGAGAAAAGGGAAAGGGA+GGG - chr8_2:6588748-6588767 None:intergenic 45.0%
AAGAGAGAAAAGGGAAAGGG+AGG - chr8_2:6588749-6588768 None:intergenic 45.0%
!! CCGTTGACTTGTCCCAATTT+GGG - chr8_2:6589122-6589141 None:intergenic 45.0%
CCCAAATTGGGACAAGTCAA+CGG + chr8_2:6589119-6589138 Msa1230560:CDS 45.0%
!! TCCGTTGACTTGTCCCAATT+TGG - chr8_2:6589123-6589142 None:intergenic 45.0%
GACATGTTGTTGGGATTTGG+TGG - chr8_2:6589210-6589229 None:intergenic 45.0%
! GCAGATTTTTCACCTGATCC+TGG + chr8_2:6589372-6589391 Msa1230560:CDS 45.0%
GGAAGTGGCTAATTCTCAAG+AGG + chr8_2:6589626-6589645 Msa1230560:CDS 45.0%
!! CTTTAGGGTGTTGAAGGTAG+TGG + chr8_2:6590070-6590089 Msa1230560:intron 45.0%
ATGGAGGAAGAGACCAAAGA+AGG + chr8_2:6590160-6590179 Msa1230560:CDS 45.0%
TGGAGGAAGAGACCAAAGAA+GGG + chr8_2:6590161-6590180 Msa1230560:CDS 45.0%
CTGCATGAATGCATGCAACA+CGG - chr8_2:6590454-6590473 None:intergenic 45.0%
GTGGTTGTTGAATCGAGATC+GGG + chr8_2:6590485-6590504 Msa1230560:intron 45.0%
CAAACGCGTTAAATCGGTGA+TGG + chr8_2:6590607-6590626 Msa1230560:intron 45.0%
GGAGGGAGAAAATAAGTGAG+AGG + chr8_2:6590787-6590806 Msa1230560:CDS 45.0%
CTTCTCCAAGATCTTGTACC+AGG + chr8_2:6590816-6590835 Msa1230560:CDS 45.0%
TCTTGTACCAGGCTGCAATA+AGG + chr8_2:6590827-6590846 Msa1230560:CDS 45.0%
CCCATAAAAGGATGGTACTG+AGG - chr8_2:6591414-6591433 None:intergenic 45.0%
GGTTGTTGCCCATAAAAGGA+TGG - chr8_2:6591422-6591441 None:intergenic 45.0%
CAGTGGGTCCCATTTGTATT+AGG - chr8_2:6591476-6591495 None:intergenic 45.0%
CATAGGAATAACGAGCCTGA+TGG - chr8_2:6591773-6591792 None:intergenic 45.0%
ACCGTTGTTCAGATGGGATT+TGG + chr8_2:6591987-6592006 Msa1230560:CDS 45.0%
GCCAAATCCCATCTGAACAA+CGG - chr8_2:6591991-6592010 None:intergenic 45.0%
! AGCAGTTTTCCTGATCCTCT+GGG - chr8_2:6592251-6592270 None:intergenic 45.0%
!! GAAAATAAATAAATAAAAAA+AGG - chr8_2:6591326-6591345 None:intergenic 5.0%
AGAAAAGGGAAAGGGAGGGA+AGG - chr8_2:6588744-6588763 None:intergenic 50.0%
! TGGTGATGATGTAGCTGCTG+GGG - chr8_2:6589190-6589209 None:intergenic 50.0%
! GTGGTGATGATGTAGCTGCT+GGG - chr8_2:6589191-6589210 None:intergenic 50.0%
GCAGCCCTTTGAACAAAACC+AGG - chr8_2:6589393-6589412 None:intergenic 50.0%
GGAGGAAGAGACCAAAGAAG+GGG + chr8_2:6590162-6590181 Msa1230560:CDS 50.0%
TGCAGCCTGGTACAAGATCT+TGG - chr8_2:6590824-6590843 None:intergenic 50.0%
! GTCATTGCAGCGTCAAGTTG+AGG + chr8_2:6591058-6591077 Msa1230560:CDS 50.0%
CTGGGGTTGTTGCCCATAAA+AGG - chr8_2:6591426-6591445 None:intergenic 50.0%
GATCCTCTGGGCTTCAATTC+AGG - chr8_2:6592239-6592258 None:intergenic 50.0%
GAATTGAAGCCCAGAGGATC+AGG + chr8_2:6592239-6592258 Msa1230560:three_prime_UTR 50.0%
! CAGCAGTTTTCCTGATCCTC+TGG - chr8_2:6592252-6592271 None:intergenic 50.0%
! GGTGGTGATGATGTAGCTGC+TGG - chr8_2:6589192-6589211 None:intergenic 55.0%
AAATGGGACCCACTGCTCAG+TGG + chr8_2:6591481-6591500 Msa1230560:CDS 55.0%
CAATGGCTCCACTGAGCAGT+GGG - chr8_2:6591492-6591511 None:intergenic 55.0%
ACGCCTGAATTGAAGCCCAG+AGG + chr8_2:6592233-6592252 Msa1230560:three_prime_UTR 55.0%
CCAATGGCTCCACTGAGCAG+TGG - chr8_2:6591493-6591512 None:intergenic 60.0%
CCACTGCTCAGTGGAGCCAT+TGG + chr8_2:6591490-6591509 Msa1230560:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 6588715 6592568 6588715 ID=Msa1230560;Name=Msa1230560
chr8_2 mRNA 6588715 6592568 6588715 ID=Msa1230560-mRNA-1;Parent=Msa1230560;Name=Msa1230560-mRNA-1;_AED=0.06;_eAED=0.06;_QI=339|1|1|1|1|1|8|348|506
chr8_2 exon 6588715 6589779 6588715 ID=Msa1230560-mRNA-1:exon:2475;Parent=Msa1230560-mRNA-1
chr8_2 exon 6590076 6590294 6590076 ID=Msa1230560-mRNA-1:exon:2476;Parent=Msa1230560-mRNA-1
chr8_2 exon 6590783 6590848 6590783 ID=Msa1230560-mRNA-1:exon:2477;Parent=Msa1230560-mRNA-1
chr8_2 exon 6591011 6591079 6591011 ID=Msa1230560-mRNA-1:exon:2478;Parent=Msa1230560-mRNA-1
chr8_2 exon 6591169 6591243 6591169 ID=Msa1230560-mRNA-1:exon:2479;Parent=Msa1230560-mRNA-1
chr8_2 exon 6591362 6591586 6591362 ID=Msa1230560-mRNA-1:exon:2480;Parent=Msa1230560-mRNA-1
chr8_2 exon 6591954 6592056 6591954 ID=Msa1230560-mRNA-1:exon:2481;Parent=Msa1230560-mRNA-1
chr8_2 exon 6592183 6592568 6592183 ID=Msa1230560-mRNA-1:exon:2482;Parent=Msa1230560-mRNA-1
chr8_2 five_prime_UTR 6588715 6589053 6588715 ID=Msa1230560-mRNA-1:five_prime_utr;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6589054 6589779 6589054 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6590076 6590294 6590076 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6590783 6590848 6590783 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6591011 6591079 6591011 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6591169 6591243 6591169 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6591362 6591586 6591362 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6591954 6592056 6591954 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 CDS 6592183 6592220 6592183 ID=Msa1230560-mRNA-1:cds;Parent=Msa1230560-mRNA-1
chr8_2 three_prime_UTR 6592221 6592568 6592221 ID=Msa1230560-mRNA-1:three_prime_utr;Parent=Msa1230560-mRNA-1
Gene Sequence

>Msa1230560

ATGGAAAACCAATTCTTTTTAAATGCTGGATTTGAAACTTACCAAACCCAAGATTGTTTTTTCAACCCAAATTGGGACAAGTCAACGGACCAAAACCTTAACTTTGATTCAGCACTTAGTTCAATTGTCTCATCCCCAGCAGCTACATCATCACCACCAAATCCCAACAACATGTCCAATGAAAATTTTGTGATGAGGGAATTGATAGGAAAATTGGGAACTTTTGGTGAGATCTCACAACATTCTTCTAACCCTTTGGTTGTACCAATGATGAACCATTTTATCAAAGAAAATATGCAAAAACCAATGTCTAATGTGGCAGATTTTTCACCTGATCCTGGTTTTGTTCAAAGGGCTGCAAAATTTTCTTGCTTTGGTAGCAAGAGTTTCAATGAGAGAGGTAACCAAATGGTGATGAACAATGTTGAGTTAGCTCAAAGATCTCATACTTTAATGGAAAATGGAATGAAGTTATCAAGAGTTTCTAGTAGTCCATCACTCAAAACATTTGGATCTCAAATGATGAACCATGAGAACAAGAATTCTCCATTGCAACAAGAAAATGATAGAATGGAAGTGGCTAATTCTCAAGAGGAATCTACAATATCTGAGCAAAATACACCAAATGGAGAAATTGGTGTCAAAGCTTCACATGATATGAATTCAAGGAAGAGAAAAGCTTCTTCTTCCAAAGGAAAAGCACCAAACTCTTCCAATCCAACAAAGGGTGTTGAAGGTAGTGGTGAAGACTTAAATGCAAAGAAAATTAAGGCAAATGAGGGTGAGAGAAATGAAAATGGTATCAAGAATATGGAGGAAGAGACCAAAGAAGGGGAAGAAAAACAGAACAAGAGTGACTCAAAACCTCCTGAACCTCAAAAAGATTACATTCATGTCAGAGCAAGAAGAGGTCAAGCTACTGACAGTCACAGTCTTGCAGAACGAGTTAGGAGGGAGAAAATAAGTGAGAGGATGAAACTTCTCCAAGATCTTGTACCAGGCTGCAATAAGGTTACTGGAAAAGCACTTATGCTTGATGAAATTATAAATTATGTTCAGTCATTGCAGCGTCAAGTTGAGTTTTTGTCTATGAAATTGTCTTCAGTGAACACAAAGATGGATTTAAGTATTGAAAGTCTTGTTGTATCAAAAGATATATTTCAATCAAATAATTCTTTGCAAAACTCAATGTTCCAATTAGATTCCTCAGTACCATCCTTTTATGGGCAACAACCCCAGCAAAACCAAGCTATTCATAATAACATTCCTAATACAAATGGGACCCACTGCTCAGTGGAGCCATTGGATTCTTCTTTGTGCCATAATAATATTGGCATGCATTTACCTTTCCTAAATGGATTTCATGAAGGTGGTTCTCAGTATCCATTAACATTCTCTGAGGAGGACTTAAACACCGTTGTTCAGATGGGATTTGGCCAAACTTCAAACAGGAATACACCAATACACTCCCCAAGTTTCAACGGTTCAAATCAATCAACACAAATGAAAATTGAGCTCTAA
Protein sequence

>Msa1230560

MENQFFLNAGFETYQTQDCFFNPNWDKSTDQNLNFDSALSSIVSSPAATSSPPNPNNMSNENFVMRELIGKLGTFGEISQHSSNPLVVPMMNHFIKENMQKPMSNVADFSPDPGFVQRAAKFSCFGSKSFNERGNQMVMNNVELAQRSHTLMENGMKLSRVSSSPSLKTFGSQMMNHENKNSPLQQENDRMEVANSQEESTISEQNTPNGEIGVKASHDMNSRKRKASSSKGKAPNSSNPTKGVEGSGEDLNAKKIKANEGERNENGIKNMEEETKEGEEKQNKSDSKPPEPQKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQRQVEFLSMKLSSVNTKMDLSIESLVVSKDIFQSNNSLQNSMFQLDSSVPSFYGQQPQQNQAIHNNIPNTNGTHCSVEPLDSSLCHNNIGMHLPFLNGFHEGGSQYPLTFSEEDLNTVVQMGFGQTSNRNTPIHSPSFNGSNQSTQMKIEL*