Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705140 | KYP58465.1 | 100.000 | 136 | 0 | 0 | 1 | 136 | 5 | 140 | 1.76e-92 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705140 | sp|P68428|H32_WHEAT | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 5.15e-95 | 272 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705140 | A0A452Z7H5 | 100.000 | 136 | 0 | 0 | 1 | 136 | 100 | 235 | 3.73e-93 | 278 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006840 | Msa0705140 | 0.842477 | 2.525012e-58 | -8.615850e-47 |
| Msa0027800 | Msa0705140 | 0.816706 | 4.816097e-52 | -8.615850e-47 |
| Msa0030070 | Msa0705140 | 0.817722 | 2.845525e-52 | -8.615850e-47 |
| Msa0033610 | Msa0705140 | 0.811616 | 6.403202e-51 | -8.615850e-47 |
| Msa0041910 | Msa0705140 | 0.810008 | 1.427254e-50 | -8.615850e-47 |
| Msa0045620 | Msa0705140 | 0.816129 | 6.484313e-52 | -8.615850e-47 |
| Msa0050530 | Msa0705140 | 0.877995 | 4.014980e-69 | -8.615850e-47 |
| Msa0078130 | Msa0705140 | 0.801893 | 7.263823e-49 | -8.615850e-47 |
| Msa0094180 | Msa0705140 | 0.814725 | 1.331155e-51 | -8.615850e-47 |
| Msa0100600 | Msa0705140 | 0.859017 | 5.530743e-63 | -8.615850e-47 |
| Msa0100890 | Msa0705140 | 0.847319 | 1.246718e-59 | -8.615850e-47 |
| Msa0109730 | Msa0705140 | 0.800428 | 1.447974e-48 | -8.615850e-47 |
| Msa0618840 | Msa0705140 | 0.842822 | 2.044740e-58 | -8.615850e-47 |
| Msa0618990 | Msa0705140 | 0.847429 | 1.163372e-59 | -8.615850e-47 |
| Msa0640800 | Msa0705140 | 0.824476 | 7.907597e-54 | -8.615850e-47 |
| Msa0647440 | Msa0705140 | 0.827369 | 1.625389e-54 | -8.615850e-47 |
| Msa0659200 | Msa0705140 | 0.864428 | 1.224990e-64 | -8.615850e-47 |
| Msa0663290 | Msa0705140 | 0.866452 | 2.821841e-65 | -8.615850e-47 |
| Msa0664070 | Msa0705140 | 0.861001 | 1.394005e-63 | -8.615850e-47 |
| Msa0684750 | Msa0705140 | 0.860111 | 2.593358e-63 | -8.615850e-47 |
| Msa0688620 | Msa0705140 | 0.819271 | 1.268022e-52 | -8.615850e-47 |
| Msa0689830 | Msa0705140 | 0.809292 | 2.033397e-50 | -8.615850e-47 |
| Msa0690320 | Msa0705140 | 0.808283 | 3.342384e-50 | -8.615850e-47 |
| Msa0705140 | Msa0705180 | 0.888905 | 3.898395e-73 | -8.615850e-47 |
| Msa0705140 | Msa0707260 | 0.831173 | 1.939555e-55 | -8.615850e-47 |
| Msa0705140 | Msa0708980 | 0.852537 | 4.327156e-61 | -8.615850e-47 |
| Msa0705140 | Msa0717900 | 0.803224 | 3.859991e-49 | -8.615850e-47 |
| Msa0705140 | Msa0720280 | 0.858213 | 9.615524e-63 | -8.615850e-47 |
| Msa0705140 | Msa0737140 | 0.815196 | 1.046305e-51 | -8.615850e-47 |
| Msa0705140 | Msa0749190 | 0.810997 | 8.725929e-51 | -8.615850e-47 |
| Msa0705140 | Msa0750520 | 0.817412 | 3.341757e-52 | -8.615850e-47 |
| Msa0705140 | Msa0751720 | 0.839071 | 1.973409e-57 | -8.615850e-47 |
| Msa0705140 | Msa0767280 | 0.829328 | 5.473538e-55 | -8.615850e-47 |
| Msa0705140 | Msa0771910 | 0.809363 | 1.963647e-50 | -8.615850e-47 |
| Msa0705140 | Msa0790330 | 0.808897 | 2.470915e-50 | -8.615850e-47 |
| Msa0705140 | Msa0803140 | 0.866864 | 2.087210e-65 | -8.615850e-47 |
| Msa0705140 | Msa0810080 | 0.802760 | 4.814328e-49 | -8.615850e-47 |
| Msa0705140 | Msa0816080 | 0.812851 | 3.443410e-51 | -8.615850e-47 |
| Msa0705140 | Msa0816230 | 0.816935 | 4.279575e-52 | -8.615850e-47 |
| Msa0705140 | Msa0831820 | 0.813436 | 2.562765e-51 | -8.615850e-47 |
| Msa0705140 | Msa0837680 | 0.842551 | 2.413637e-58 | -8.615850e-47 |
| Msa0705140 | Msa0841500 | 0.854596 | 1.108171e-61 | -8.615850e-47 |
| Msa0705140 | Msa0846900 | 0.871912 | 4.761150e-67 | -8.615850e-47 |
| Msa0705140 | Msa0850600 | 0.808991 | 2.359423e-50 | -8.615850e-47 |
| Msa0705140 | Msa0856610 | 0.846959 | 1.564872e-59 | -8.615850e-47 |
| Msa0705140 | Msa0861440 | 0.833926 | 4.027919e-56 | -8.615850e-47 |
| Msa0705140 | Msa0874730 | 0.812996 | 3.200849e-51 | -8.615850e-47 |
| Msa0705140 | Msa0900090 | 0.821807 | 3.317176e-53 | -8.615850e-47 |
| Msa0705140 | Msa0926310 | 0.811780 | 5.899753e-51 | -8.615850e-47 |
| Msa0705140 | Msa0926760 | 0.843411 | 1.424292e-58 | -8.615850e-47 |
| Msa0705140 | Msa0929900 | 0.855612 | 5.614615e-62 | -8.615850e-47 |
| Msa0705140 | Msa0965470 | 0.805740 | 1.153902e-49 | -8.615850e-47 |
| Msa0705140 | Msa0982960 | 0.843073 | 1.752811e-58 | -8.615850e-47 |
| Msa0705140 | Msa1000780 | 0.820900 | 5.372615e-53 | -8.615850e-47 |
| Msa0705140 | Msa1021560 | 0.818166 | 2.258418e-52 | -8.615850e-47 |
| Msa0705140 | Msa1041370 | 0.814062 | 1.865518e-51 | -8.615850e-47 |
| Msa0705140 | Msa1060300 | 0.825610 | 4.268772e-54 | -8.615850e-47 |
| Msa0705140 | Msa1071410 | 0.820009 | 8.600551e-53 | -8.615850e-47 |
| Msa0705140 | Msa1078860 | 0.800987 | 1.113406e-48 | -8.615850e-47 |
| Msa0705140 | Msa1078990 | 0.830312 | 3.152221e-55 | -8.615850e-47 |
| Msa0705140 | Msa1081270 | 0.809810 | 1.574052e-50 | -8.615850e-47 |
| Msa0705140 | Msa1083630 | 0.802960 | 4.377889e-49 | -8.615850e-47 |
| Msa0705140 | Msa1085680 | 0.850452 | 1.683011e-60 | -8.615850e-47 |
| Msa0705140 | Msa1098060 | 0.841636 | 4.215052e-58 | -8.615850e-47 |
| Msa0705140 | Msa1105540 | 0.821129 | 4.757840e-53 | -8.615850e-47 |
| Msa0705140 | Msa1105580 | 0.811296 | 7.514440e-51 | -8.615850e-47 |
| Msa0705140 | Msa1106220 | 0.808251 | 3.395385e-50 | -8.615850e-47 |
| Msa0705140 | Msa1128620 | 0.804082 | 2.562012e-49 | -8.615850e-47 |
| Msa0705140 | Msa1144990 | 0.854137 | 1.503790e-61 | -8.615850e-47 |
| Msa0705140 | Msa1147560 | 0.810437 | 1.153141e-50 | -8.615850e-47 |
| Msa0705140 | Msa1148930 | 0.800872 | 1.175270e-48 | -8.615850e-47 |
| Msa0705140 | Msa1162360 | 0.834446 | 2.983084e-56 | -8.615850e-47 |
| Msa0705140 | Msa1169170 | 0.814368 | 1.596775e-51 | -8.615850e-47 |
| Msa0705140 | Msa1191000 | 0.812548 | 4.012162e-51 | -8.615850e-47 |
| Msa0705140 | Msa1244690 | 0.801894 | 7.260314e-49 | -8.615850e-47 |
| Msa0705140 | Msa1282300 | 0.804540 | 2.057115e-49 | -8.615850e-47 |
| Msa0705140 | Msa1294790 | 0.800922 | 1.147944e-48 | -8.615850e-47 |
| Msa0705140 | Msa1301820 | 0.816507 | 5.336191e-52 | -8.615850e-47 |
| Msa0705140 | Msa1301830 | 0.828207 | 1.022148e-54 | -8.615850e-47 |
| Msa0705140 | Msa1339230 | 0.870797 | 1.112050e-66 | -8.615850e-47 |
| Msa0705140 | Msa1371800 | 0.805322 | 1.411712e-49 | -8.615850e-47 |
| Msa0705140 | Msa1372030 | 0.806904 | 6.556937e-50 | -8.615850e-47 |
| Msa0705140 | Msa1376310 | 0.804601 | 1.997658e-49 | -8.615850e-47 |
| Msa0705140 | Msa1381800 | 0.821573 | 3.758830e-53 | -8.615850e-47 |
| Msa0705140 | Msa1390210 | 0.821988 | 3.012307e-53 | -8.615850e-47 |
| Msa0705140 | Msa1393560 | 0.811446 | 6.974381e-51 | -8.615850e-47 |
| Msa0705140 | Msa1420830 | 0.834860 | 2.347115e-56 | -8.615850e-47 |
| Msa0705140 | Msa1424370 | 0.816279 | 6.003305e-52 | -8.615850e-47 |
| Msa0705140 | Msa1431520 | 0.802613 | 5.163344e-49 | -8.615850e-47 |
| Msa0705140 | Msa1460970 | 0.800251 | 1.573521e-48 | -8.615850e-47 |
| Msa0705140 | Msa1463430 | 0.805028 | 1.626404e-49 | -8.615850e-47 |
| Msa0125590 | Msa0705140 | 0.826930 | 2.069964e-54 | -8.615850e-47 |
| Msa0136330 | Msa0705140 | 0.807905 | 4.023424e-50 | -8.615850e-47 |
| Msa0168380 | Msa0705140 | 0.824932 | 6.173814e-54 | -8.615850e-47 |
| Msa0186180 | Msa0705140 | 0.836351 | 9.844036e-57 | -8.615850e-47 |
| Msa0186560 | Msa0705140 | 0.815443 | 9.220278e-52 | -8.615850e-47 |
| Msa0204970 | Msa0705140 | 0.845283 | 4.472148e-59 | -8.615850e-47 |
| Msa0225800 | Msa0705140 | 0.807804 | 4.225418e-50 | -8.615850e-47 |
| Msa0364680 | Msa0705140 | 0.801606 | 8.317769e-49 | -8.615850e-47 |
| Msa0383940 | Msa0705140 | 0.826929 | 2.071400e-54 | -8.615850e-47 |
| Msa0385730 | Msa0705140 | 0.819094 | 1.391152e-52 | -8.615850e-47 |
| Msa0388620 | Msa0705140 | 0.813733 | 2.204460e-51 | -8.615850e-47 |
| Msa0416210 | Msa0705140 | 0.808644 | 2.798115e-50 | -8.615850e-47 |
| Msa0420430 | Msa0705140 | 0.805999 | 1.017925e-49 | -8.615850e-47 |
| Msa0429970 | Msa0705140 | 0.834653 | 2.646096e-56 | -8.615850e-47 |
| Msa0433630 | Msa0705140 | 0.828393 | 9.219797e-55 | -8.615850e-47 |
| Msa0434360 | Msa0705140 | 0.810471 | 1.134154e-50 | -8.615850e-47 |
| Msa0477480 | Msa0705140 | 0.812748 | 3.627624e-51 | -8.615850e-47 |
| Msa0478140 | Msa0705140 | 0.806805 | 6.880835e-50 | -8.615850e-47 |
| Msa0478200 | Msa0705140 | 0.808457 | 3.068679e-50 | -8.615850e-47 |
| Msa0249870 | Msa0705140 | 0.841249 | 5.327147e-58 | -8.615850e-47 |
| Msa0260570 | Msa0705140 | 0.833334 | 5.661522e-56 | -8.615850e-47 |
| Msa0281540 | Msa0705140 | 0.827412 | 1.587108e-54 | -8.615850e-47 |
| Msa0288400 | Msa0705140 | 0.829256 | 5.697760e-55 | -8.615850e-47 |
| Msa0320290 | Msa0705140 | 0.809670 | 1.686783e-50 | -8.615850e-47 |
| Msa0320580 | Msa0705140 | 0.804680 | 1.922744e-49 | -8.615850e-47 |
| Msa0342220 | Msa0705140 | 0.825197 | 5.345538e-54 | -8.615850e-47 |
| Msa0344110 | Msa0705140 | 0.812844 | 3.455876e-51 | -8.615850e-47 |
| Msa0506880 | Msa0705140 | 0.800949 | 1.133551e-48 | -8.615850e-47 |
| Msa0514020 | Msa0705140 | 0.802603 | 5.186513e-49 | -8.615850e-47 |
| Msa0520680 | Msa0705140 | 0.800804 | 1.213565e-48 | -8.615850e-47 |
| Msa0521510 | Msa0705140 | 0.820899 | 5.374079e-53 | -8.615850e-47 |
| Msa0521530 | Msa0705140 | 0.826024 | 3.402758e-54 | -8.615850e-47 |
| Msa0522300 | Msa0705140 | 0.807137 | 5.853302e-50 | -8.615850e-47 |
| Msa0527850 | Msa0705140 | 0.831289 | 1.815927e-55 | -8.615850e-47 |
| Msa0541740 | Msa0705140 | 0.802311 | 5.958687e-49 | -8.615850e-47 |
| Msa0544410 | Msa0705140 | 0.833540 | 5.029197e-56 | -8.615850e-47 |
| Msa0544430 | Msa0705140 | 0.816327 | 5.855655e-52 | -8.615850e-47 |
| Msa0554300 | Msa0705140 | 0.806485 | 8.040064e-50 | -8.615850e-47 |
| Msa0571350 | Msa0705140 | 0.806308 | 8.760606e-50 | -8.615850e-47 |
| Msa0572830 | Msa0705140 | 0.849470 | 3.168298e-60 | -8.615850e-47 |
| Msa0576330 | Msa0705140 | 0.849997 | 2.256505e-60 | -8.615850e-47 |
| Msa0582460 | Msa0705140 | 0.835913 | 1.272033e-56 | -8.615850e-47 |
| Msa0586200 | Msa0705140 | 0.827186 | 1.798200e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705140 | MtrunA17_Chr5g0410931 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr5g0410951 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr8g0383851 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr8g0383781 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr8g0390361 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr2g0295191 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr7g0237661 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr4g0033041 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr7g0218971 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr4g0046301 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr8g0364801 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0705140 | MtrunA17_Chr4g0054161 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0705140 | MtrunA17_Chr8g0363741 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0705140 | MtrunA17_Chr1g0156021 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0705140 | MtrunA17_Chr4g0054151 | 97.059 | 136 | 4 | 0 | 1 | 136 | 4 | 139 | 2.61e-93 | 265 |
| Msa0705140 | MtrunA17_Chr8g0388121 | 96.324 | 136 | 5 | 0 | 1 | 136 | 1 | 136 | 1.09e-92 | 264 |
| Msa0705140 | MtrunA17_Chr2g0319141 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0705140 | MtrunA17_Chr2g0319151 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0705140 | MtrunA17_Chr2g0319131 | 85.616 | 146 | 11 | 1 | 1 | 136 | 1 | 146 | 1.38e-82 | 238 |
| Msa0705140 | MtrunA17_Chr2g0319121 | 84.559 | 136 | 19 | 2 | 1 | 135 | 1 | 135 | 5.82e-75 | 219 |
| Msa0705140 | MtrunA17_Chr7g0218961 | 86.607 | 112 | 15 | 0 | 25 | 136 | 2 | 113 | 8.01e-66 | 195 |
| Msa0705140 | MtrunA17_Chr7g0222991 | 67.164 | 134 | 43 | 1 | 1 | 134 | 10 | 142 | 5.47e-56 | 171 |
| Msa0705140 | MtrunA17_Chr5g0407311 | 67.241 | 116 | 35 | 2 | 1 | 116 | 1 | 113 | 1.29e-45 | 144 |
| Msa0705140 | MtrunA17_Chr7g0223001 | 58.647 | 133 | 41 | 3 | 3 | 134 | 27 | 146 | 5.46e-43 | 139 |
| Msa0705140 | MtrunA17_Chr7g0240791 | 100.000 | 66 | 0 | 0 | 1 | 66 | 1 | 66 | 9.61e-41 | 133 |
| Msa0705140 | MtrunA17_Chr8g0347231 | 55.102 | 98 | 43 | 1 | 37 | 133 | 28 | 125 | 3.80e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705140 | AT1G09200.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0705140 | AT5G65360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0705140 | AT5G10390.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0705140 | AT5G10400.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0705140 | AT3G27360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0705140 | AT4G40030.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0705140 | AT4G40030.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0705140 | AT4G40030.4 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0705140 | AT5G10980.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0705140 | AT4G40040.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0705140 | AT4G40040.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0705140 | AT4G40040.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0705140 | AT4G40030.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 29 | 164 | 7.05e-93 | 266 |
| Msa0705140 | AT5G65350.1 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 5.30e-91 | 260 |
| Msa0705140 | AT1G75600.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.25e-89 | 256 |
| Msa0705140 | AT1G13370.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 5.55e-89 | 254 |
| Msa0705140 | AT1G19890.1 | 89.781 | 137 | 13 | 1 | 1 | 136 | 1 | 137 | 1.07e-83 | 241 |
| Msa0705140 | AT5G12910.1 | 71.111 | 135 | 34 | 2 | 1 | 135 | 1 | 130 | 9.90e-67 | 198 |
| Msa0705140 | AT1G01370.2 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0705140 | AT1G01370.1 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0705140 | AT1G01370.4 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0705140 | AT1G01370.3 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
Find 32 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGGTTGCCAGTTGCTTC+CGG | 0.239809 | 4_4:-77708228 | None:intergenic |
| TTCTTCACTCCTCCGGTTGC+CGG | 0.280449 | 4_4:-77708268 | None:intergenic |
| TTTGGTTGCCAGTTGCTTCC+GGG | 0.293951 | 4_4:-77708227 | None:intergenic |
| TCTCTCAAAGCAACTGTTCC+AGG | 0.333816 | 4_4:-77708307 | None:intergenic |
| ATTCCAGAGCAGTGCTGTTT+CGG | 0.340103 | 4_4:+77708429 | Msa0705140:CDS |
| CAAGGCTCCCCGGAAGCAAC+TGG | 0.376550 | 4_4:+77708219 | Msa0705140:CDS |
| CGAAAATCAGCTCCGGCAAC+CGG | 0.399036 | 4_4:+77708256 | Msa0705140:CDS |
| AGAAGCGGCTGAAGCTTACT+TGG | 0.416086 | 4_4:+77708459 | Msa0705140:CDS |
| GAGCCGAAACAGCACTGCTC+TGG | 0.422418 | 4_4:-77708432 | None:intergenic |
| GACGTTGGAATGGAAGTTTA+CGG | 0.458530 | 4_4:-77708366 | None:intergenic |
| CTTGCAAGCTGAATGTCCTT+AGG | 0.462646 | 4_4:-77708541 | None:intergenic |
| GTGTGGCTTCTTCACTCCTC+CGG | 0.469362 | 4_4:-77708275 | None:intergenic |
| CCACCGGCGGCAAGGCTCCC+CGG | 0.480154 | 4_4:+77708209 | Msa0705140:CDS |
| TCGCAAGTCCACCGGCGGCA+AGG | 0.488583 | 4_4:+77708201 | Msa0705140:CDS |
| TTGGTTGCCAGTTGCTTCCG+GGG | 0.522282 | 4_4:-77708226 | None:intergenic |
| AGCCGCCCGAAAATCAGCTC+CGG | 0.530760 | 4_4:+77708249 | Msa0705140:CDS |
| CTTCCGGGGAGCCTTGCCGC+CGG | 0.543290 | 4_4:-77708212 | None:intergenic |
| CCGGGGAGCCTTGCCGCCGG+TGG | 0.548377 | 4_4:-77708209 | None:intergenic |
| GGACTTGCGAGCAGTCTGCT+TGG | 0.551577 | 4_4:-77708188 | None:intergenic |
| CAGACTGCTCGCAAGTCCAC+CGG | 0.562052 | 4_4:+77708193 | Msa0705140:CDS |
| GCGGCTGAAGCTTACTTGGT+CGG | 0.565776 | 4_4:+77708463 | Msa0705140:CDS |
| GAGAGTTACAATCATGCCTA+AGG | 0.570792 | 4_4:+77708525 | Msa0705140:CDS |
| AAGAAGAATCAGAGGAGAGA+GGG | 0.573886 | 4_4:+77708561 | Msa0705140:CDS |
| TCACGAACAAGACGTTGGAA+TGG | 0.598115 | 4_4:-77708376 | None:intergenic |
| CAAGAAGAATCAGAGGAGAG+AGG | 0.603703 | 4_4:+77708560 | Msa0705140:CDS |
| CGATTTCACGAACAAGACGT+TGG | 0.613448 | 4_4:-77708381 | None:intergenic |
| CAGCTTGCAAGAAGAATCAG+AGG | 0.621034 | 4_4:+77708553 | Msa0705140:CDS |
| AAATCAGCTCCGGCAACCGG+AGG | 0.622487 | 4_4:+77708259 | Msa0705140:CDS |
| ACTGCTCGCAAGTCCACCGG+CGG | 0.647164 | 4_4:+77708196 | Msa0705140:CDS |
| AAGCCACACAGATTCAGACC+TGG | 0.672238 | 4_4:+77708289 | Msa0705140:CDS |
| TGTTTCGGCTCTTCAAGAAG+CGG | 0.672573 | 4_4:+77708444 | Msa0705140:CDS |
| GTTCCAGGTCTGAATCTGTG+TGG | 0.688220 | 4_4:-77708292 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GACGTTGGAATGGAAGTTTA+CGG | - | chr4_4:77708369-77708388 | None:intergenic | 40.0% | |
| GAGAGTTACAATCATGCCTA+AGG | + | chr4_4:77708525-77708544 | Msa0705140:CDS | 40.0% | |
| AAGAAGAATCAGAGGAGAGA+GGG | + | chr4_4:77708561-77708580 | Msa0705140:CDS | 40.0% | |
| TCTCTCAAAGCAACTGTTCC+AGG | - | chr4_4:77708310-77708329 | None:intergenic | 45.0% | |
| TCACGAACAAGACGTTGGAA+TGG | - | chr4_4:77708379-77708398 | None:intergenic | 45.0% | |
| CGATTTCACGAACAAGACGT+TGG | - | chr4_4:77708384-77708403 | None:intergenic | 45.0% | |
| !! | ATTCCAGAGCAGTGCTGTTT+CGG | + | chr4_4:77708429-77708448 | Msa0705140:CDS | 45.0% |
| TGTTTCGGCTCTTCAAGAAG+CGG | + | chr4_4:77708444-77708463 | Msa0705140:CDS | 45.0% | |
| CTTGCAAGCTGAATGTCCTT+AGG | - | chr4_4:77708544-77708563 | None:intergenic | 45.0% | |
| CAGCTTGCAAGAAGAATCAG+AGG | + | chr4_4:77708553-77708572 | Msa0705140:CDS | 45.0% | |
| CAAGAAGAATCAGAGGAGAG+AGG | + | chr4_4:77708560-77708579 | Msa0705140:CDS | 45.0% | |
| TTTGGTTGCCAGTTGCTTCC+GGG | - | chr4_4:77708230-77708249 | None:intergenic | 50.0% | |
| CTTTGGTTGCCAGTTGCTTC+CGG | - | chr4_4:77708231-77708250 | None:intergenic | 50.0% | |
| AAGCCACACAGATTCAGACC+TGG | + | chr4_4:77708289-77708308 | Msa0705140:CDS | 50.0% | |
| GTTCCAGGTCTGAATCTGTG+TGG | - | chr4_4:77708295-77708314 | None:intergenic | 50.0% | |
| AGAAGCGGCTGAAGCTTACT+TGG | + | chr4_4:77708459-77708478 | Msa0705140:CDS | 50.0% | |
| TTGGTTGCCAGTTGCTTCCG+GGG | - | chr4_4:77708229-77708248 | None:intergenic | 55.0% | |
| !! | AGCTGATTTTCGGGCGGCTT+TGG | - | chr4_4:77708248-77708267 | None:intergenic | 55.0% |
| !! | GGTTGCCGGAGCTGATTTTC+GGG | - | chr4_4:77708257-77708276 | None:intergenic | 55.0% |
| !! | CGGTTGCCGGAGCTGATTTT+CGG | - | chr4_4:77708258-77708277 | None:intergenic | 55.0% |
| CGAAAATCAGCTCCGGCAAC+CGG | + | chr4_4:77708256-77708275 | Msa0705140:CDS | 55.0% | |
| TTCTTCACTCCTCCGGTTGC+CGG | - | chr4_4:77708271-77708290 | None:intergenic | 55.0% | |
| GTGTGGCTTCTTCACTCCTC+CGG | - | chr4_4:77708278-77708297 | None:intergenic | 55.0% | |
| GCGGCTGAAGCTTACTTGGT+CGG | + | chr4_4:77708463-77708482 | Msa0705140:CDS | 55.0% | |
| GGACTTGCGAGCAGTCTGCT+TGG | - | chr4_4:77708191-77708210 | None:intergenic | 60.0% | |
| CAGACTGCTCGCAAGTCCAC+CGG | + | chr4_4:77708193-77708212 | Msa0705140:CDS | 60.0% | |
| AGCCGCCCGAAAATCAGCTC+CGG | + | chr4_4:77708249-77708268 | Msa0705140:CDS | 60.0% | |
| !! | TGCCGGAGCTGATTTTCGGG+CGG | - | chr4_4:77708254-77708273 | None:intergenic | 60.0% |
| AAATCAGCTCCGGCAACCGG+AGG | + | chr4_4:77708259-77708278 | Msa0705140:CDS | 60.0% | |
| GAGCCGAAACAGCACTGCTC+TGG | - | chr4_4:77708435-77708454 | None:intergenic | 60.0% | |
| ACTGCTCGCAAGTCCACCGG+CGG | + | chr4_4:77708196-77708215 | Msa0705140:CDS | 65.0% | |
| CAAGGCTCCCCGGAAGCAAC+TGG | + | chr4_4:77708219-77708238 | Msa0705140:CDS | 65.0% | |
| TCGCAAGTCCACCGGCGGCA+AGG | + | chr4_4:77708201-77708220 | Msa0705140:CDS | 70.0% | |
| CTTCCGGGGAGCCTTGCCGC+CGG | - | chr4_4:77708215-77708234 | None:intergenic | 75.0% | |
| ! | CCACCGGCGGCAAGGCTCCC+CGG | + | chr4_4:77708209-77708228 | Msa0705140:CDS | 80.0% |
| ! | CCGGGGAGCCTTGCCGCCGG+TGG | - | chr4_4:77708212-77708231 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 77708178 | 77708588 | 77708178 | ID=Msa0705140;Name=Msa0705140 |
| chr4_4 | mRNA | 77708178 | 77708588 | 77708178 | ID=Msa0705140-mRNA-1;Parent=Msa0705140;Name=Msa0705140-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr4_4 | exon | 77708178 | 77708588 | 77708178 | ID=Msa0705140-mRNA-1:exon:24736;Parent=Msa0705140-mRNA-1 |
| chr4_4 | CDS | 77708178 | 77708588 | 77708178 | ID=Msa0705140-mRNA-1:cds;Parent=Msa0705140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
