AlfalfaGEDB | Msa0766760

M. sativa cultivar zhongmu-4 / Msa0766760

KEGG: K13424
GO: GO:0001067 GO:0001101 GO:0002831 GO:0002832 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009753 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043900 GO:0043901 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0140110 GO:1900150 GO:1900424 GO:1900425 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766760	sp\|Q9XI90\|WRKY4_ARATH	49.234	522	159	16	8	433	3	514	3.64e-134	398

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766760	G7JWM9	92.857	434	29	2	1	433	1	433	0.0	829

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TFs/TRs:

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Gene ID	Type	Classification
Msa0766760	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007990	Msa0766760	0.801689	7.998662e-49	-8.615850e-47
Msa0042550	Msa0766760	0.802773	4.784276e-49	-8.615850e-47
Msa0064110	Msa0766760	0.802592	5.215748e-49	-8.615850e-47
Msa0071250	Msa0766760	0.817546	3.117856e-52	-8.615850e-47
Msa0092410	Msa0766760	0.830837	2.344803e-55	-8.615850e-47
Msa0103250	Msa0766760	0.805226	1.478920e-49	-8.615850e-47
Msa0106620	Msa0766760	0.830721	2.503330e-55	-8.615850e-47
Msa0106710	Msa0766760	0.822220	2.662100e-53	-8.615850e-47
Msa0138020	Msa0766760	0.831853	1.319075e-55	-8.615850e-47
Msa0149300	Msa0766760	0.806564	7.736592e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766760	MtrunA17_Chr5g0418191	92.857	434	29	2	1	433	1	433	0.0	829
Msa0766760	MtrunA17_Chr4g0042831	55.011	449	110	12	14	432	2	388	1.59e-142	412
Msa0766760	MtrunA17_Chr7g0218081	49.414	512	153	15	21	432	14	519	7.73e-137	402
Msa0766760	MtrunA17_Chr5g0445311	50.000	298	128	7	143	429	191	478	3.97e-81	259
Msa0766760	MtrunA17_Chr7g0263531	49.270	274	117	5	137	397	174	438	5.70e-81	256
Msa0766760	MtrunA17_Chr3g0101131	51.064	282	121	6	144	414	235	510	8.33e-81	260
Msa0766760	MtrunA17_Chr3g0101131	49.425	87	39	3	135	218	398	482	2.72e-16	81.3
Msa0766760	MtrunA17_Chr3g0079691	45.507	345	121	9	137	415	262	605	5.20e-80	261
Msa0766760	MtrunA17_Chr3g0106491	51.575	254	111	4	141	388	187	434	6.92e-78	250
Msa0766760	MtrunA17_Chr7g0243891	54.251	247	99	5	149	393	213	447	1.67e-77	250

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0766760	AT1G13960.1	49.234	522	159	16	8	433	3	514	3.70e-135	398
Msa0766760	AT2G03340.1	54.412	408	114	10	71	433	133	513	1.89e-130	386
Msa0766760	AT2G03340.1	65.957	47	12	2	24	66	20	66	7.16e-11	64.7
Msa0766760	AT1G13960.2	57.143	371	116	10	96	433	1	361	1.18e-121	358
Msa0766760	AT3G01080.2	43.215	479	169	17	22	433	18	460	1.34e-92	287
Msa0766760	AT3G01080.1	43.946	446	175	15	22	433	18	422	1.11e-89	278
Msa0766760	AT5G56270.1	48.667	300	108	4	129	382	250	549	1.07e-79	260
Msa0766760	AT2G38470.1	53.759	266	93	7	149	395	181	435	4.75e-79	254
Msa0766760	AT4G26640.5	52.846	246	96	4	151	385	34	270	3.92e-77	245
Msa0766760	AT4G26640.5	48.052	77	39	1	135	210	188	264	1.62e-14	75.1

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Find 129 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTAGGCTTATCAGAAA+TGG	0.435730	5_2:+28868825	None:intergenic
AAAAGTGCGTCCGTACGTGG+CGG	0.684333	5_2:+28872586	None:intergenic
AAAAGTTGGTGGAGATAACA+GGG	0.561299	5_2:-28868571	Msa0766760:three_prime_UTR
AAACCAAGTTGTATGTTCAA+CGG	0.645642	5_2:-28872701	Msa0766760:five_prime_UTR
AAAGTTGGTGGAGATAACAG+GGG	0.637324	5_2:-28868570	Msa0766760:three_prime_UTR
AACAACCTGTTGCTCGTCTA+AGG	0.334127	5_2:-28868771	Msa0766760:CDS
AAGCTCCTAAATTAAGACTC+TGG	0.554251	5_2:-28868726	Msa0766760:three_prime_UTR
AAGGTTGAGCGTTCGCTTGC+TGG	0.338256	5_2:-28869958	Msa0766760:CDS
AAGTTAGAGAGAAGAGTCAT+AGG	0.429317	5_2:+28872541	None:intergenic
AATGAAAATTCAAATAGGCA+AGG	0.563913	5_2:-28869850	Msa0766760:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGATAACAAGACAAACTCAA+AGG	-	chr5_2:28868445-28868464	Msa0766760:three_prime_UTR	30.0%
AAACCAAGTTGTATGTTCAA+CGG	-	chr5_2:28868600-28868619	Msa0766760:three_prime_UTR	30.0%
ATAAACAAAGATTTCACCTG+AGG	+	chr5_2:28868898-28868917	None:intergenic	30.0%
CTATCAACTTCTACTTACAT+GGG	+	chr5_2:28869541-28869560	None:intergenic	30.0%
ACTATCAACTTCTACTTACA+TGG	+	chr5_2:28869542-28869561	None:intergenic	30.0%
TTGGATCACTATATCAAATG+AGG	+	chr5_2:28869574-28869593	None:intergenic	30.0%
CGGCTAAAAAATGATTTGAT+TGG	+	chr5_2:28869695-28869714	None:intergenic	30.0%
TTGGTAAAAGCTCTTAAATC+TGG	-	chr5_2:28869809-28869828	Msa0766760:CDS	30.0%
TGGTAAAAGCTCTTAAATCT+GGG	-	chr5_2:28869810-28869829	Msa0766760:CDS	30.0%
TACTAACAGACAAGTAAATG+TGG	+	chr5_2:28869862-28869881	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	28868414	28872909	28868414	ID=Msa0766760;Name=Msa0766760
chr5_2	mRNA	28868414	28872909	28868414	ID=Msa0766760-mRNA-1;Parent=Msa0766760;Name=Msa0766760-mRNA-1;_AED=0.00;_eAED=0.00;_QI=214\|1\|1\|1\|1\|1\|4\|333\|433
chr5_2	exon	28868414	28869029	28868414	ID=Msa0766760-mRNA-1:exon:11090;Parent=Msa0766760-mRNA-1
chr5_2	exon	28869286	28869444	28869286	ID=Msa0766760-mRNA-1:exon:11089;Parent=Msa0766760-mRNA-1
chr5_2	exon	28869679	28870265	28869679	ID=Msa0766760-mRNA-1:exon:11088;Parent=Msa0766760-mRNA-1
chr5_2	exon	28872423	28872909	28872423	ID=Msa0766760-mRNA-1:exon:11087;Parent=Msa0766760-mRNA-1
chr5_2	five_prime_UTR	28872696	28872909	28872696	ID=Msa0766760-mRNA-1:five_prime_utr;Parent=Msa0766760-mRNA-1
chr5_2	CDS	28872423	28872695	28872423	ID=Msa0766760-mRNA-1:cds;Parent=Msa0766760-mRNA-1
chr5_2	CDS	28869679	28870265	28869679	ID=Msa0766760-mRNA-1:cds;Parent=Msa0766760-mRNA-1
chr5_2	CDS	28869286	28869444	28869286	ID=Msa0766760-mRNA-1:cds;Parent=Msa0766760-mRNA-1
chr5_2	CDS	28868747	28869029	28868747	ID=Msa0766760-mRNA-1:cds;Parent=Msa0766760-mRNA-1
chr5_2	three_prime_UTR	28868414	28868746	28868414	ID=Msa0766760-mRNA-1:three_prime_utr;Parent=Msa0766760-mRNA-1

Gene Sequence

>Msa0766760

ATGGACCGGCCACCGTGGAAGGAAGAAGAAGAAGCGGCAATTGGCGGTGGCGAAGGGGGACCACCACAGCGACCCACCTTAACTCTTCCGCCACGTACGGACGCACTTTTCACCGGTGGTTTTAGCCCTGGTCCTATGACTCTTCTCTCTAACTTATTTGCTGATGGTGATGACGGCAAGTCCTTCTCTCAGCTTCTCGCCGGTGCCACGGTGTCTCCGGCAACGGTAACCACCGGTGGGTTTGATTCATCTGGTTTCTTTTCACATCCTCAGGTATCCTTTGGAATGACACAGCAGCAACCACTGGCACAGGTCTCGGCTCACGAAGGACAATCCAACACTAATATGCTTAACCAAGCCGAACATTTATCGTCTGTACCAGCATCCACCGATCACGCTCTTCTCTCCGAACAAAGGTTGCAGCCTTCTTCGTTGAATATCGATAAGCCTGCCGATGATGGCTACAATTGGAGGAAATATGGACAGAAACAGGTGAAAGGTAGTGAGTTTCCTCGAAGTTATTACAAGTGCACGCATCCTAACTGTCCTGTCAAGAAAAAGGTTGAGCGTTCGCTTGCTGGTCATATAACCGCAATTATTTATAAAGGAGAGCACAATCATCAACTTCCAAATCCTAATAAGCGATCAAAAGACACTATAACTTCAAATGAAAATTCAAATAGGCAAGGGAGTGCTGATTCAACTTATCAACGGATGACTTCAAACTCAATGTCTAAGATGGACCCTGAATCTAGTCAGGCAACAGGTGAACATCTATCTGGAACGAGTGATAGTGAGGATGTAGGTGATCGTGAAACAGAAGTGGATGAGAAAAGAATTGAGCCCGACTCCAAAAGGAGACATACAGAAGTCACAGTCTCAAATCCAACAACTTCACATAGTACTGTCACAGAGCCTAAAATCATCGTGCAAACTACCAGTGAAGTTGATCTCTTAGATGATGGTTATAGATGGCGAAAATATGGACAGAAAGTTGTTAAAGGAAATCCTTATCCTAGGAGCTATTACAAATGTACAACACCGAGTTGTAACGTTCGAAAGCATGTAGAGAGAGCATCGACAGATCCTAAAGCAGTCATAACAACATATGAAGGAAAACATAACCATGATGTTCCAGCAGCTAAGACTAATAGCCACACTATTGCCAACAATAATGCTTCACAGTTAAAATCGCAAAATACCATTTCTGATAAGCCTAGTTTTGGTAGCATTGGTATTGGCGGGGGCAATGAGCAACAACCTGTTGCTCGTCTAAGGCTGAAGGAAGAGCAGATAACTTAA

Protein sequence

>Msa0766760

MDRPPWKEEEEAAIGGGEGGPPQRPTLTLPPRTDALFTGGFSPGPMTLLSNLFADGDDGKSFSQLLAGATVSPATVTTGGFDSSGFFSHPQVSFGMTQQQPLAQVSAHEGQSNTNMLNQAEHLSSVPASTDHALLSEQRLQPSSLNIDKPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLAGHITAIIYKGEHNHQLPNPNKRSKDTITSNENSNRQGSADSTYQRMTSNSMSKMDPESSQATGEHLSGTSDSEDVGDRETEVDEKRIEPDSKRRHTEVTVSNPTTSHSTVTEPKIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPSCNVRKHVERASTDPKAVITTYEGKHNHDVPAAKTNSHTIANNNASQLKSQNTISDKPSFGSIGIGGGNEQQPVARLRLKEEQIT*