AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1372280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1372280 MtrunA17_Chr2g0279151 98.221 281 5 0 1 281 1 281 0.0 571
Msa1372280 MtrunA17_Chr4g0050381 79.004 281 55 1 1 281 1 277 6.70e-169 468
Msa1372280 MtrunA17_Chr5g0426141 74.823 282 70 1 1 281 1 282 1.67e-154 432
Msa1372280 MtrunA17_Chr3g0115851 73.404 282 74 1 1 281 1 282 3.72e-154 431
Msa1372280 MtrunA17_Chr2g0317021 61.049 267 103 1 16 281 67 333 7.86e-112 326
Msa1372280 MtrunA17_Chr8g0360351 61.255 271 104 1 11 280 11 281 3.56e-110 320
Msa1372280 MtrunA17_Chr4g0051661 54.217 249 113 1 32 280 150 397 9.27e-88 266
Msa1372280 MtrunA17_Chr1g0150451 46.183 262 134 5 22 280 66 323 2.03e-70 220
Msa1372280 MtrunA17_Chr3g0136711 45.660 265 131 6 22 280 23 280 5.03e-69 216
Msa1372280 MtrunA17_Chr1g0212961 41.729 266 141 7 22 280 79 337 1.41e-64 206
Msa1372280 MtrunA17_Chr3g0126511 45.149 268 140 5 16 280 77 340 1.89e-64 206
Msa1372280 MtrunA17_Chr3g0105371 37.052 251 142 8 41 281 95 339 3.65e-39 139
Msa1372280 MtrunA17_Chr5g0430481 38.776 245 135 7 47 281 136 375 4.77e-39 140
Msa1372280 MtrunA17_Chr3g0105351 37.302 252 142 8 40 281 70 315 1.57e-38 137
Msa1372280 MtrunA17_Chr1g0187481 32.453 265 153 5 23 280 32 277 2.49e-36 130
Msa1372280 MtrunA17_Chr1g0194191 34.800 250 134 7 51 278 105 347 3.82e-36 132
Msa1372280 MtrunA17_Chr5g0407161 34.498 229 132 5 64 280 123 345 9.78e-36 131
Msa1372280 MtrunA17_Chr1g0166481 34.831 267 144 5 21 280 30 273 2.87e-35 127
Msa1372280 MtrunA17_Chr3g0144111 36.187 257 111 8 47 280 1 227 1.30e-34 125
Msa1372280 MtrunA17_Chr4g0070871 37.743 257 115 7 65 281 253 504 6.28e-34 129
Msa1372280 MtrunA17_Chr3g0138281 35.345 232 131 5 64 281 120 346 2.24e-33 125
Msa1372280 MtrunA17_Chr2g0293811 33.898 236 128 7 65 278 100 329 8.07e-33 123
Msa1372280 MtrunA17_Chr4g0065141 32.950 261 142 9 47 281 98 351 9.48e-33 124
Msa1372280 MtrunA17_Chr1g0171571 32.770 296 166 10 8 281 361 645 2.77e-31 122
Msa1372280 MtrunA17_Chr6g0483411 34.286 245 127 9 66 281 80 319 2.63e-30 115
Msa1372280 MtrunA17_Chr2g0299981 33.951 324 135 12 31 281 219 536 1.93e-29 116
Msa1372280 MtrunA17_Chr3g0112291 32.069 290 151 10 33 281 92 376 1.38e-27 109
Msa1372280 MtrunA17_Chr4g0001761 29.283 321 147 13 30 281 20 329 2.44e-27 108
Msa1372280 MtrunA17_Chr1g0150071 27.358 318 154 10 32 281 22 330 9.88e-27 107
Msa1372280 MtrunA17_Chr8g0389921 34.016 244 132 7 63 281 196 435 1.09e-26 108
Msa1372280 MtrunA17_Chr1g0150781 33.086 269 128 9 58 281 283 544 2.27e-26 108
Msa1372280 MtrunA17_Chr3g0136811 27.358 318 154 10 32 281 22 330 8.53e-26 104
Msa1372280 MtrunA17_Chr1g0158781 33.574 277 156 8 29 281 108 380 1.01e-25 104
Msa1372280 MtrunA17_Chr3g0135961 31.203 266 137 7 58 281 279 540 1.01e-25 106
Msa1372280 MtrunA17_Chr5g0435721 33.766 231 132 6 33 246 192 418 5.26e-24 100
Msa1372280 MtrunA17_Chr5g0396731 31.282 195 117 4 65 246 107 297 6.82e-22 93.6
Msa1372280 MtrunA17_Chr7g0272061 38.547 179 83 9 125 278 183 359 1.96e-21 92.8
Msa1372280 MtrunA17_Chr1g0192421 35.349 215 109 11 91 280 141 350 5.89e-21 90.9
Msa1372280 MtrunA17_Chr4g0067711 36.275 204 103 10 102 280 151 352 8.19e-21 90.9
Msa1372280 MtrunA17_Chr7g0263281 28.276 290 129 10 58 278 64 343 2.25e-20 89.7
Msa1372280 MtrunA17_Chr2g0295401 37.500 216 105 12 90 280 143 353 6.49e-20 88.2
Msa1372280 MtrunA17_Chr1g0209451 28.030 264 159 10 40 278 38 295 9.71e-20 88.2
Msa1372280 MtrunA17_Chr3g0127091 27.090 299 156 11 31 277 58 346 1.27e-19 87.8
Msa1372280 MtrunA17_Chr6g0486661 28.016 257 141 10 65 278 124 379 2.06e-19 88.2
Msa1372280 MtrunA17_Chr1g0181901 29.911 224 114 7 65 247 76 297 2.37e-19 87.0
Msa1372280 MtrunA17_Chr3g0089711 28.528 326 153 12 23 278 29 344 4.73e-19 85.9
Msa1372280 MtrunA17_Chr3g0089721 28.528 326 153 12 23 278 29 344 4.88e-19 85.9
Msa1372280 MtrunA17_Chr8g0371321 28.000 275 129 10 68 278 82 351 6.01e-19 85.9
Msa1372280 MtrunA17_Chr1g0213351 28.223 287 134 11 58 278 72 352 1.15e-18 84.7
Msa1372280 MtrunA17_Chr7g0256771 28.063 253 121 10 40 246 53 290 1.29e-18 84.0
Msa1372280 MtrunA17_Chr1g0153671 29.185 233 141 9 65 278 65 292 2.23e-18 84.3
Msa1372280 MtrunA17_Chr1g0211251 29.204 226 113 8 65 247 81 302 4.04e-18 83.6
Msa1372280 MtrunA17_Chr7g0221691 38.122 181 90 10 102 264 208 384 1.08e-17 82.4
Msa1372280 MtrunA17_Chr7g0250061 28.315 279 129 12 65 278 76 348 1.86e-17 81.6
Msa1372280 MtrunA17_Chr7g0225501 31.513 238 137 11 63 278 84 317 5.83e-17 80.5
Msa1372280 MtrunA17_Chr4g0004971 40.000 160 77 7 124 266 165 322 6.27e-17 79.7
Msa1372280 MtrunA17_Chr2g0324061 36.420 162 85 7 102 246 162 322 1.09e-16 79.7
Msa1372280 MtrunA17_Chr4g0068971 38.333 120 59 3 169 278 115 229 1.43e-16 77.8
Msa1372280 MtrunA17_Chr7g0271971 27.660 235 146 8 63 278 66 295 2.87e-16 78.2
Msa1372280 MtrunA17_Chr7g0249421 36.420 162 85 7 102 246 183 343 4.93e-16 77.8
Msa1372280 MtrunA17_Chr1g0192471 25.818 275 177 9 25 278 27 295 8.22e-16 77.0
Msa1372280 MtrunA17_Chr7g0238501 27.778 252 131 9 68 270 266 515 1.05e-15 77.0
Msa1372280 MtrunA17_Chr7g0243571 35.669 157 85 6 125 266 188 343 1.25e-15 76.6
Msa1372280 MtrunA17_Chr3g0133801 27.778 234 143 9 65 278 66 293 1.34e-15 76.3
Msa1372280 MtrunA17_Chr7g0256751 26.101 318 153 13 33 280 42 347 7.34e-15 74.3
Msa1372280 MtrunA17_Chr2g0294291 27.237 257 141 10 57 270 66 319 1.46e-14 73.2
Msa1372280 MtrunA17_Chr1g0207571 25.407 307 158 12 36 278 36 335 1.25e-13 70.5
Msa1372280 MtrunA17_Chr1g0151781 29.289 239 128 10 53 251 55 292 7.11e-13 67.8
Msa1372280 MtrunA17_Chr8g0342431 36.913 149 76 6 132 264 203 349 8.19e-13 68.2
Msa1372280 MtrunA17_Chr4g0063051 28.947 228 138 11 60 270 55 275 1.19e-12 67.0
Msa1372280 MtrunA17_Chr1g0175381 25.436 287 152 13 33 270 53 326 2.37e-11 63.5
Msa1372280 MtrunA17_Chr3g0089691 27.984 243 106 9 101 278 20 258 7.69e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1372280 AT1G34750.2 70.567 282 82 1 1 281 1 282 9.30e-146 410
Msa1372280 AT1G34750.1 70.567 282 82 1 1 281 1 282 9.30e-146 410
Msa1372280 AT1G34750.3 70.567 282 82 1 1 281 1 282 9.30e-146 410
Msa1372280 AT1G22280.1 68.327 281 89 0 1 281 1 281 4.55e-139 393
Msa1372280 AT1G22280.3 66.899 287 89 1 1 281 1 287 1.72e-136 387
Msa1372280 AT1G78200.1 62.324 284 103 3 1 281 1 283 2.04e-125 358
Msa1372280 AT1G78200.3 62.324 284 103 3 1 281 1 283 2.04e-125 358
Msa1372280 AT1G78200.2 62.324 284 103 3 1 281 1 283 2.04e-125 358
Msa1372280 AT1G34750.4 68.016 247 76 2 1 246 1 245 5.02e-119 341
Msa1372280 AT1G78200.5 67.227 238 75 2 47 281 1 238 1.35e-112 325
Msa1372280 AT1G78200.4 67.227 238 75 2 47 281 1 238 1.35e-112 325
Msa1372280 AT2G20630.1 59.023 266 106 2 19 281 14 279 1.47e-110 321
Msa1372280 AT4G28400.1 58.427 267 106 2 19 280 16 282 9.59e-109 317
Msa1372280 AT3G15260.2 60.517 271 105 2 13 281 19 289 2.39e-108 316
Msa1372280 AT3G15260.1 60.517 271 105 2 13 281 19 289 2.39e-108 316
Msa1372280 AT4G28400.3 59.073 259 105 1 23 280 2 260 2.52e-108 315
Msa1372280 AT2G20630.2 58.491 265 107 2 19 280 14 278 1.27e-107 314
Msa1372280 AT4G28400.2 57.589 224 90 2 19 237 16 239 1.26e-87 262
Msa1372280 AT2G34740.1 53.200 250 111 3 34 280 88 334 5.93e-84 255
Msa1372280 AT1G22280.2 68.108 185 59 0 1 185 1 185 9.30e-83 247
Msa1372280 AT5G10740.1 47.547 265 126 6 22 280 22 279 6.46e-73 228
Msa1372280 AT5G10740.2 47.547 265 126 6 22 280 22 279 6.46e-73 228
Msa1372280 AT5G24940.1 46.241 266 130 6 21 280 21 279 8.10e-71 225
Msa1372280 AT2G34740.3 54.106 207 89 3 34 237 84 287 1.59e-70 220
Msa1372280 AT2G34740.2 54.106 207 89 3 34 237 102 305 1.62e-70 221
Msa1372280 AT4G31750.1 45.420 262 136 5 22 280 22 279 4.25e-70 219
Msa1372280 AT4G31750.3 45.420 262 136 5 22 280 22 279 4.25e-70 219
Msa1372280 AT4G31750.2 45.420 262 136 5 22 280 22 279 4.56e-70 219
Msa1372280 AT5G53140.1 43.511 262 141 5 23 281 91 348 5.40e-65 209
Msa1372280 AT5G53140.2 43.511 262 141 5 23 281 91 348 5.40e-65 209
Msa1372280 AT1G43900.1 41.502 253 140 6 32 281 122 369 3.30e-59 193
Msa1372280 AT3G51470.2 34.661 251 143 7 45 280 39 283 1.56e-39 140
Msa1372280 AT2G33700.1 35.448 268 146 8 33 280 84 344 5.79e-39 140
Msa1372280 AT3G51470.1 34.661 251 143 7 45 280 83 327 7.79e-39 139
Msa1372280 AT2G30020.1 40.400 250 130 11 45 281 150 393 1.09e-38 140
Msa1372280 AT3G62260.3 38.494 239 120 8 64 280 26 259 3.47e-38 136
Msa1372280 AT3G62260.1 38.494 239 120 8 64 280 115 348 2.69e-37 136
Msa1372280 AT3G62260.2 38.494 239 120 8 64 280 116 349 2.98e-37 136
Msa1372280 AT1G07160.1 39.442 251 130 9 45 280 133 376 4.71e-37 135
Msa1372280 AT2G40180.1 36.653 251 138 8 47 281 141 386 3.01e-36 133
Msa1372280 AT2G25620.1 36.325 234 124 6 64 278 127 354 4.21e-36 133
Msa1372280 AT2G25620.2 36.325 234 124 6 64 278 127 354 4.21e-36 133
Msa1372280 AT1G48040.1 34.201 269 136 10 45 280 89 349 3.62e-33 125
Msa1372280 AT3G17250.1 33.607 244 130 6 63 280 156 393 5.97e-33 125
Msa1372280 AT1G67820.2 35.841 226 131 8 47 265 97 315 2.19e-31 120
Msa1372280 AT1G67820.1 35.841 226 131 8 47 265 133 351 6.57e-31 120
Msa1372280 AT5G57050.2 34.109 258 124 9 65 281 120 372 8.40e-30 116
Msa1372280 AT5G57050.1 34.109 258 124 9 65 281 160 412 9.24e-30 116
Msa1372280 AT4G26080.1 33.115 305 142 13 31 281 127 423 2.41e-29 115
Msa1372280 AT2G40860.4 33.333 249 150 6 46 281 306 551 3.47e-27 110
Msa1372280 AT2G40860.1 33.333 249 150 6 46 281 404 649 3.62e-27 110
Msa1372280 AT1G07430.1 34.034 238 129 8 33 246 120 353 3.75e-27 109
Msa1372280 AT3G11410.1 33.032 221 113 6 56 247 128 342 4.16e-27 108
Msa1372280 AT5G51760.2 33.333 318 148 11 24 281 69 382 6.50e-27 108
Msa1372280 AT2G29380.1 32.031 256 127 8 66 281 113 361 6.87e-27 107
Msa1372280 AT1G18030.1 31.481 270 147 11 45 281 85 349 7.04e-27 107
Msa1372280 AT5G51760.1 33.333 318 148 11 24 281 99 412 8.00e-27 108
Msa1372280 AT2G25070.1 27.445 317 155 11 32 281 22 330 6.45e-26 105
Msa1372280 AT2G25070.2 27.445 317 155 11 32 281 22 330 6.45e-26 105
Msa1372280 AT1G72770.4 42.222 180 78 5 125 281 326 502 8.87e-26 106
Msa1372280 AT1G72770.1 42.222 180 78 5 125 281 326 502 8.87e-26 106
Msa1372280 AT1G72770.3 42.222 180 78 5 125 281 326 502 8.87e-26 106
Msa1372280 AT2G40860.2 33.051 236 142 6 46 268 404 636 8.14e-25 104
Msa1372280 AT4G31860.1 27.044 318 155 11 32 281 22 330 1.83e-24 101
Msa1372280 AT5G59220.1 32.340 235 134 6 33 246 111 341 3.00e-24 101
Msa1372280 AT3G23360.1 28.627 255 146 6 31 280 36 259 4.36e-24 98.2
Msa1372280 AT1G17550.1 31.227 269 130 7 65 281 237 502 6.59e-24 101
Msa1372280 AT1G17550.2 31.227 269 130 7 65 281 237 502 6.59e-24 101
Msa1372280 AT4G31860.3 28.102 274 129 10 32 246 22 286 6.96e-23 95.9
Msa1372280 AT4G27800.1 28.852 305 153 11 27 277 51 345 7.42e-23 97.1
Msa1372280 AT3G16800.4 40.104 192 88 10 115 281 65 254 1.58e-22 94.0
Msa1372280 AT3G16800.6 40.104 192 88 10 115 281 65 254 1.58e-22 94.0
Msa1372280 AT1G18030.2 32.579 221 116 9 45 237 85 300 2.29e-22 94.7
Msa1372280 AT3G05640.2 37.255 204 101 9 102 280 148 349 2.32e-22 95.5
Msa1372280 AT3G05640.1 37.255 204 101 9 102 280 148 349 2.32e-22 95.5
Msa1372280 AT3G05640.3 37.255 204 101 9 102 280 148 349 2.32e-22 95.5
Msa1372280 AT3G16800.2 40.104 192 88 10 115 281 160 349 3.74e-22 94.7
Msa1372280 AT3G16800.1 40.104 192 88 10 115 281 160 349 3.74e-22 94.7
Msa1372280 AT3G16800.3 40.104 192 88 10 115 281 160 349 3.74e-22 94.7
Msa1372280 AT5G27930.1 36.634 202 101 10 102 278 153 352 4.86e-21 92.0
Msa1372280 AT5G27930.2 36.634 202 101 10 102 278 153 352 4.86e-21 92.0
Msa1372280 AT4G31860.2 27.757 263 122 10 32 235 22 275 4.98e-20 87.8
Msa1372280 AT4G08260.1 30.165 242 129 9 47 281 1 209 5.79e-20 86.3
Msa1372280 AT3G12620.2 30.851 282 130 11 59 278 77 355 5.87e-20 89.0
Msa1372280 AT3G12620.1 30.851 282 130 11 59 278 77 355 5.87e-20 89.0
Msa1372280 AT1G68410.4 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa1372280 AT1G68410.3 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa1372280 AT1G68410.1 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa1372280 AT1G68410.2 26.562 256 162 9 43 278 51 300 1.08e-19 88.6
Msa1372280 AT4G27800.2 27.336 289 149 11 27 265 51 328 2.03e-19 87.0
Msa1372280 AT3G51370.1 29.084 251 131 8 65 270 79 327 2.16e-19 87.4
Msa1372280 AT3G51370.3 29.084 251 131 8 65 270 79 327 2.16e-19 87.4
Msa1372280 AT2G20050.1 28.740 254 140 10 65 278 143 395 2.26e-19 88.6
Msa1372280 AT4G27800.3 27.985 268 133 10 27 244 51 308 2.31e-19 86.7
Msa1372280 AT2G20050.2 28.740 254 140 10 65 278 143 395 2.51e-19 88.2
Msa1372280 AT1G09160.2 27.239 268 168 9 32 278 34 295 3.59e-19 87.0
Msa1372280 AT1G09160.1 27.239 268 168 9 32 278 34 295 3.59e-19 87.0
Msa1372280 AT1G47380.1 26.953 256 161 9 43 278 42 291 1.22e-18 85.5
Msa1372280 AT5G66080.1 29.084 251 125 9 68 270 85 330 3.26e-18 84.0
Msa1372280 AT3G27140.1 30.992 242 107 8 47 281 1 189 4.17e-18 82.0
Msa1372280 AT4G38520.2 28.571 280 136 11 63 280 78 355 4.37e-18 84.0
Msa1372280 AT4G38520.1 28.571 280 136 11 63 280 78 355 4.37e-18 84.0
Msa1372280 AT5G06750.3 29.110 292 139 10 43 272 50 335 2.59e-17 81.6
Msa1372280 AT5G06750.1 29.110 292 139 10 43 272 50 335 2.59e-17 81.6
Msa1372280 AT5G01700.1 29.839 248 134 10 58 266 21 267 2.62e-17 80.9
Msa1372280 AT3G55050.1 25.979 281 145 8 59 278 78 356 2.78e-17 81.3
Msa1372280 AT3G55050.2 25.979 281 145 8 59 278 78 356 2.78e-17 81.3
Msa1372280 AT5G01700.2 29.839 248 134 10 58 266 70 316 3.37e-17 81.3
Msa1372280 AT1G79630.2 34.066 182 102 7 102 266 128 308 8.90e-17 80.1
Msa1372280 AT1G79630.7 34.066 182 102 7 102 266 128 308 8.90e-17 80.1
Msa1372280 AT1G79630.1 34.066 182 102 7 102 266 198 378 1.09e-16 80.1
Msa1372280 AT3G02750.2 35.556 180 92 8 90 246 174 352 1.17e-16 80.1
Msa1372280 AT3G02750.1 35.556 180 92 8 90 246 174 352 1.17e-16 80.1
Msa1372280 AT1G79630.8 34.066 182 102 7 102 266 194 374 1.23e-16 80.1
Msa1372280 AT1G79630.6 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa1372280 AT1G79630.3 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa1372280 AT1G79630.4 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa1372280 AT1G79630.5 34.066 182 102 7 102 266 88 268 1.51e-16 79.3
Msa1372280 AT5G02760.1 28.125 288 141 12 21 247 9 291 1.93e-16 79.0
Msa1372280 AT1G16220.1 36.478 159 84 6 124 266 198 355 6.27e-16 77.8
Msa1372280 AT3G17090.1 27.113 284 143 10 57 278 75 356 1.92e-15 75.9
Msa1372280 AT3G51370.2 30.660 212 101 7 103 270 33 242 1.97e-15 75.1
Msa1372280 AT5G36250.1 34.973 183 99 8 102 266 183 363 1.00e-14 74.3
Msa1372280 AT3G17090.2 26.724 232 128 7 57 247 75 305 1.66e-14 72.8
Msa1372280 AT5G66080.2 30.189 212 102 7 103 270 17 226 2.74e-14 71.6
Msa1372280 AT2G40860.3 30.769 195 120 5 46 228 404 595 9.17e-14 71.6
Msa1372280 AT2G05050.1 39.394 99 54 2 189 281 92 190 3.33e-13 67.4
Msa1372280 AT5G26010.1 27.935 247 130 10 37 246 47 282 4.41e-13 68.9
Msa1372280 AT4G33920.1 27.148 291 134 13 58 278 54 336 6.08e-13 68.6
Msa1372280 AT5G57050.3 32.680 153 83 7 65 202 160 307 6.34e-13 68.2
Msa1372280 AT4G32950.1 30.638 235 129 11 58 264 65 293 6.50e-13 68.2
Msa1372280 AT5G19280.1 25.784 287 171 12 28 280 299 577 7.40e-13 68.9
Msa1372280 AT5G59220.2 30.000 190 108 6 33 201 111 296 2.56e-12 66.2
Msa1372280 AT5G27930.3 36.424 151 76 8 102 234 153 301 9.54e-12 64.7
Msa1372280 AT5G36250.2 32.487 197 99 9 102 266 183 377 1.25e-11 65.1
Msa1372280 AT5G19280.2 25.253 297 170 13 28 280 299 587 1.73e-11 64.7

Find 83 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCACAGTCCTGATTT+GGG 0.253672 tig0019276:-33023 Msa1372280:CDS
TCTTTCTCACAGTCCTGATT+TGG 0.254999 tig0019276:-33024 Msa1372280:CDS
TGAGAGAGAGTTGAATTAAA+TGG 0.263700 tig0019276:-34161 Msa1372280:exon
ATGAGAGGTAAGATCTTTAT+AGG 0.272783 tig0019276:+34358 None:intergenic
TAATAAATAATCAGAAATTA+TGG 0.279094 tig0019276:-32968 Msa1372280:CDS
ATGAAATCAATTTATCAGTT+TGG 0.285612 tig0019276:-31429 Msa1372280:three_prime_UTR
GGTAAGATCTTTATAGGAAG+TGG 0.290720 tig0019276:+34364 None:intergenic
TGAAGCTCTTCGATTCAATT+AGG 0.301274 tig0019276:-31358 Msa1372280:three_prime_UTR
CTTCAAGGCCCTCTTAAGTA+TGG 0.331523 tig0019276:-33381 Msa1372280:CDS
CTACCAAAGAGCTTATTAAT+AGG 0.340721 tig0019276:+34485 None:intergenic
ATGATGCGTCTTTCAGTATT+GGG 0.345245 tig0019276:+32870 None:intergenic
TTTCCTATTAATAAGCTCTT+TGG 0.346720 tig0019276:-34488 Msa1372280:five_prime_UTR
CTTGCAGTTGCTCGGGCATT+CGG 0.354027 tig0019276:-31833 Msa1372280:CDS
GGAAAGAATTTGTAAGCATC+TGG 0.358340 tig0019276:+34506 None:intergenic
TTTATATTGACAATGGGAAT+TGG 0.367194 tig0019276:-31230 Msa1372280:three_prime_UTR
ATGGCTGCAAAACACAAATA+AGG 0.374341 tig0019276:+34594 None:intergenic
CCAACTCTCGCCCGTTGAAC+TGG 0.390268 tig0019276:+33295 None:intergenic
GGCAAAGGCAAGAGCCTTCA+AGG 0.390525 tig0019276:-33396 Msa1372280:CDS
GATGATGCGTCTTTCAGTAT+TGG 0.404290 tig0019276:+32869 None:intergenic
GATCCTCCACGCCCCAAATC+AGG 0.407496 tig0019276:+33011 None:intergenic
CATACTTAAGAGGGCCTTGA+AGG 0.416633 tig0019276:+33382 None:intergenic
TACTTGCTAGTGATGGTCTA+TGG 0.418352 tig0019276:-31730 Msa1372280:CDS
GCTTCGATGATCGAACTGTT+TGG 0.423468 tig0019276:+33071 None:intergenic
CACAGTCCTGATTTGGGGCG+TGG 0.426527 tig0019276:-33017 Msa1372280:CDS
GTATCAAGAGCTCAGTATCT+TGG 0.427375 tig0019276:+31750 None:intergenic
TTTCTGATTATTTATTAGAA+TGG 0.428271 tig0019276:+32974 None:intergenic
CAGATCGCAAGTGTGACTTC+AGG 0.437990 tig0019276:+31801 None:intergenic
AGACGCATCATCGAGAATAA+AGG 0.440708 tig0019276:-32858 Msa1372280:CDS
AGCTTATGAGGCAACTGATC+AGG 0.443548 tig0019276:-33051 Msa1372280:CDS
AAGCAAAAGCCATACTTAAG+AGG 0.443765 tig0019276:+33372 None:intergenic
CCTTCAAGATATTAGAAAAG+AGG 0.445161 tig0019276:+33211 None:intergenic
CGAAAATCGTCCAGTTCAAC+GGG 0.446953 tig0019276:-33305 Msa1372280:CDS
CAGTTCAACGGGCGAGAGTT+GGG 0.457156 tig0019276:-33294 Msa1372280:CDS
TTGCAATATCAACTGCCTCT+TGG 0.459676 tig0019276:+31589 None:intergenic
TGCTATATTTGATGGACACT+CGG 0.459898 tig0019276:-33265 Msa1372280:CDS
GGACGTTCTTTATGCGGCTC+TGG 0.465744 tig0019276:-33417 Msa1372280:CDS
TGGACAGCTTGCAGTTGCTC+GGG 0.484799 tig0019276:-31840 Msa1372280:CDS
GTAAGATCTTTATAGGAAGT+GGG 0.489143 tig0019276:+34365 None:intergenic
ATGGACAGCTTGCAGTTGCT+CGG 0.494076 tig0019276:-31841 Msa1372280:CDS
GGTGATGTTGCAAGAGTGAA+TGG 0.495091 tig0019276:-31860 Msa1372280:CDS
CCAGTTCAACGGGCGAGAGT+TGG 0.504156 tig0019276:-33295 Msa1372280:CDS
GTATCTTGGTCAATATCGTC+GGG 0.509507 tig0019276:+31764 None:intergenic
GCGAAAATCGTCCAGTTCAA+CGG 0.511342 tig0019276:-33306 Msa1372280:CDS
AGTATCTTGGTCAATATCGT+CGG 0.513251 tig0019276:+31763 None:intergenic
TTCGATCATCGAAGCTTATG+AGG 0.516217 tig0019276:-33063 Msa1372280:CDS
AGGCATTGAAAAGAGAGAGC+AGG 0.521221 tig0019276:-31521 Msa1372280:CDS
AAAGATAAAAGATCCACAAA+AGG 0.522450 tig0019276:-31565 Msa1372280:CDS
GTTGCGGAAATGAAGAGAAA+AGG 0.523684 tig0019276:+34410 None:intergenic
TCTTTATGCGGCTCTGGCAA+AGG 0.527519 tig0019276:-33411 Msa1372280:CDS
TTTCACTGAATTGATCATCT+GGG 0.532697 tig0019276:-34293 Msa1372280:five_prime_UTR
GGCATTGAAAAGAGAGAGCA+GGG 0.537795 tig0019276:-31520 Msa1372280:CDS
TTATAGGAAGTGGGTGGTCA+CGG 0.537871 tig0019276:+34374 None:intergenic
AAATTATGGATAGCAAATGT+TGG 0.546553 tig0019276:-32954 Msa1372280:CDS
CTCTTGATACTTGCTAGTGA+TGG 0.551258 tig0019276:-31737 Msa1372280:CDS
GCTATATTTGATGGACACTC+GGG 0.552095 tig0019276:-33264 Msa1372280:CDS
AGAAATCAAGAAATACTGAA+TGG 0.557197 tig0019276:+31330 None:intergenic
AGCTTGAGTAACTTGATCCT+CGG 0.566493 tig0019276:-31190 Msa1372280:three_prime_UTR
GTTGGAGGACGTTCTTTATG+CGG 0.569210 tig0019276:-33423 Msa1372280:CDS
GCAAAACACAAATAAGGAAG+AGG 0.569566 tig0019276:+34600 None:intergenic
TTTCTCACAGTCCTGATTTG+GGG 0.570400 tig0019276:-33022 Msa1372280:CDS
AGCAAAAGCCATACTTAAGA+GGG 0.571518 tig0019276:+33373 None:intergenic
CGAGCAGTTCTGTCGAGGAA+AGG 0.583792 tig0019276:-32924 Msa1372280:CDS
AGCTCTTCGATTCAATTAGG+TGG 0.590001 tig0019276:-31355 Msa1372280:three_prime_UTR
TTGCTTCAACTCTTCCCGAA+AGG 0.594951 tig0019276:-34128 Msa1372280:intron
TGCTAGTGATGGTCTATGGA+AGG 0.596717 tig0019276:-31726 Msa1372280:intron
GGTTTATTAAAGAACCTGGA+AGG 0.597472 tig0019276:+32820 None:intergenic
TTTGGGGCGTGGAGGATCGA+CGG 0.597691 tig0019276:-33006 Msa1372280:CDS
TCATCTGAGTGAAAATTCAG+AGG 0.600464 tig0019276:-34258 Msa1372280:five_prime_UTR
CTTGAAGCGAACTACAATGC+AGG 0.608420 tig0019276:+31488 None:intergenic
TCTTCATTTCCGCAACAGAA+CGG 0.618247 tig0019276:-34403 Msa1372280:five_prime_UTR
AGTCCTGATTTGGGGCGTGG+AGG 0.620509 tig0019276:-33014 Msa1372280:CDS
ATTCACGAGCAGTTCTGTCG+AGG 0.639885 tig0019276:-32929 Msa1372280:CDS
TCAGGTAATGGCAAACCAAG+AGG 0.643063 tig0019276:-31604 Msa1372280:intron
AGTCGCTTATATTATAGCCG+AGG 0.645750 tig0019276:+31173 None:intergenic
AGCTAAACAACTAACTGCAG+AGG 0.651227 tig0019276:-31541 Msa1372280:CDS
CGTGAAAGTCCTCCATTGTG+TGG 0.653511 tig0019276:+33331 None:intergenic
GACACAAGACCACACAATGG+AGG 0.665307 tig0019276:-33340 Msa1372280:CDS
AGTTCTGTCGAGGAAAGGTG+TGG 0.670672 tig0019276:-32919 Msa1372280:CDS
AGCAGTGTGAAGATGAACAT+TGG 0.673038 tig0019276:-31255 Msa1372280:three_prime_UTR
CGCATCATCGAGAATAAAGG+CGG 0.678594 tig0019276:-32855 Msa1372280:CDS
TGGGAATTGGAATACATGAG+TGG 0.686678 tig0019276:-31217 Msa1372280:three_prime_UTR
AGATCTTTATAGGAAGTGGG+TGG 0.704685 tig0019276:+34368 None:intergenic
AGGGACACAAGACCACACAA+TGG 0.718438 tig0019276:-33343 Msa1372280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTTAAATTTTTTGAT+TGG - tig0019276:31447-31466 Msa1372280:three_prime_UTR 10.0%
!! TAATAAATAATCAGAAATTA+TGG - tig0019276:32805-32824 Msa1372280:intron 10.0%
!!! TAATTACAAAAAATTGTTTT+GGG + tig0019276:33530-33549 None:intergenic 10.0%
!!! ACTTTTATTTTGTGTTTTTT+TGG - tig0019276:31681-31700 Msa1372280:intron 15.0%
!! AGCTATTAAAAACTAAATTT+GGG + tig0019276:31810-31829 None:intergenic 15.0%
!! AAGCTATTAAAAACTAAATT+TGG + tig0019276:31811-31830 None:intergenic 15.0%
!! AACAACCAAAATATAATATA+TGG + tig0019276:32176-32195 None:intergenic 15.0%
!! TTTCTGATTATTTATTAGAA+TGG + tig0019276:32802-32821 None:intergenic 15.0%
!!! CTAATTACAAAAAATTGTTT+TGG + tig0019276:33531-33550 None:intergenic 15.0%
!!! CAAAACAATTTTTTGTAATT+AGG - tig0019276:33529-33548 Msa1372280:intron 15.0%
!!! CCTACTTATAAATATATTTT+AGG + tig0019276:33691-33710 None:intergenic 15.0%
!! CCTAAAATATATTTATAAGT+AGG - tig0019276:33688-33707 Msa1372280:intron 15.0%
!! TTATCATAAGTTCAAAATTT+AGG + tig0019276:33757-33776 None:intergenic 15.0%
!!! TAAATTTTGAACTTATGATA+AGG - tig0019276:33756-33775 Msa1372280:intron 15.0%
!!! AATAATTTTAGAAACAATTG+CGG + tig0019276:34104-34123 None:intergenic 15.0%
!!! AAAGTCTTTTGTTTTTTCAA+TGG + tig0019276:31201-31220 None:intergenic 20.0%
!! TACAACATATAACAAAACAA+TGG + tig0019276:32600-32619 None:intergenic 20.0%
!! TTATATGTTGTAATGTCTTT+CGG - tig0019276:32608-32627 Msa1372280:intron 20.0%
!!! AACAATTTTTTGTAATTAGG+AGG - tig0019276:33532-33551 Msa1372280:intron 20.0%
!! TATGATAAGGTTTATTTAGA+TGG - tig0019276:33769-33788 Msa1372280:intron 20.0%
!! GACATTAGAAGATAAATTTA+AGG + tig0019276:34082-34101 None:intergenic 20.0%
!!! TCAATTGATTACATATTTTC+AGG - tig0019276:34151-34170 Msa1372280:exon 20.0%
!! ATGAAATCAATTTATCAGTT+TGG - tig0019276:34344-34363 Msa1372280:five_prime_UTR 20.0%
!!! ATTGGTTTTTATATTGACAA+TGG - tig0019276:34536-34555 Msa1372280:five_prime_UTR 20.0%
!!! TTGGTTTTTATATTGACAAT+GGG - tig0019276:34537-34556 Msa1372280:five_prime_UTR 20.0%
! AAAAATGCAAAAAGTCAACA+AGG - tig0019276:31146-31165 Msa1372280:three_prime_UTR 25.0%
! AAAATGCAAAAAGTCAACAA+GGG - tig0019276:31147-31166 Msa1372280:three_prime_UTR 25.0%
! TTTCCTATTAATAAGCTCTT+TGG - tig0019276:31285-31304 Msa1372280:three_prime_UTR 25.0%
!! TTTGTTCTTTCTTTTGTCTT+TGG - tig0019276:31724-31743 Msa1372280:intron 25.0%
!!! TTTCTTTTGTCTTTGGAATA+TGG - tig0019276:31731-31750 Msa1372280:CDS 25.0%
! TAATTCTAACGTTGAGAAAT+TGG + tig0019276:31764-31783 None:intergenic 25.0%
!! TCAACGTTAGAATTAAGTTT+TGG - tig0019276:31769-31788 Msa1372280:CDS 25.0%
!!! AATTAAGTTTTGGTGATTTG+AGG - tig0019276:31779-31798 Msa1372280:CDS 25.0%
! TGATGAACAAAATTGAAACT+TGG - tig0019276:31834-31853 Msa1372280:CDS 25.0%
! GATGAACAAAATTGAAACTT+GGG - tig0019276:31835-31854 Msa1372280:CDS 25.0%
!!! TTATATTTTGGTTGTTGTAC+TGG - tig0019276:32180-32199 Msa1372280:intron 25.0%
!! GTTTAGTCTTCAACATAAAA+TGG + tig0019276:32213-32232 None:intergenic 25.0%
! CATAACATAAATGTCAATGT+TGG + tig0019276:32235-32254 None:intergenic 25.0%
! ACATTGACATTTATGTTATG+TGG - tig0019276:32235-32254 Msa1372280:intron 25.0%
!!! TGCTAGTAATTTGTTAAAAG+TGG - tig0019276:32280-32299 Msa1372280:intron 25.0%
!!! ATTTTAACTTGCAACGTTTT+AGG - tig0019276:32325-32344 Msa1372280:intron 25.0%
!!! TTTTAACTTGCAACGTTTTA+GGG - tig0019276:32326-32345 Msa1372280:intron 25.0%
!!! AAAGAGGTGTTTTTGTAAAT+AGG + tig0019276:32549-32568 None:intergenic 25.0%
! AAAATCTCACAAGTTATGAT+TGG - tig0019276:32652-32671 Msa1372280:intron 25.0%
! AAATTATGGATAGCAAATGT+TGG - tig0019276:32819-32838 Msa1372280:intron 25.0%
! TTAAGGTTTATTAAAGAACC+TGG + tig0019276:32960-32979 None:intergenic 25.0%
! CAATATCTCATAAGACATTA+AGG + tig0019276:32977-32996 None:intergenic 25.0%
!! TACATCTTTTATGGTTCATA+AGG - tig0019276:33178-33197 Msa1372280:intron 25.0%
! GATTCTACACTTTATGTTAT+AGG + tig0019276:33242-33261 None:intergenic 25.0%
! AATTAGAGCATGATTAAACT+GGG + tig0019276:33306-33325 None:intergenic 25.0%
! AAATTAGAGCATGATTAAAC+TGG + tig0019276:33307-33326 None:intergenic 25.0%
! GCATATAGTTATATCAAGTT+GGG + tig0019276:33397-33416 None:intergenic 25.0%
! AGCATATAGTTATATCAAGT+TGG + tig0019276:33398-33417 None:intergenic 25.0%
! AACTATATGCTAGAGAATAA+AGG - tig0019276:33407-33426 Msa1372280:CDS 25.0%
! ACTATATGCTAGAGAATAAA+GGG - tig0019276:33408-33427 Msa1372280:CDS 25.0%
!!! GATTACATATTTTCAGGTAA+TGG - tig0019276:34157-34176 Msa1372280:exon 25.0%
! AAAGATAAAAGATCCACAAA+AGG - tig0019276:34208-34227 Msa1372280:five_prime_UTR 25.0%
!! TGAATTTTACATGGGAAAAA+GGG + tig0019276:34392-34411 None:intergenic 25.0%
!! CTTCAAACTGAATTTTACAT+GGG + tig0019276:34400-34419 None:intergenic 25.0%
! AGAAATCAAGAAATACTGAA+TGG + tig0019276:34446-34465 None:intergenic 25.0%
! TTTATATTGACAATGGGAAT+TGG - tig0019276:34543-34562 Msa1372280:five_prime_UTR 25.0%
CTACCAAAGAGCTTATTAAT+AGG + tig0019276:31291-31310 None:intergenic 30.0%
GTAAGATCTTTATAGGAAGT+GGG + tig0019276:31411-31430 None:intergenic 30.0%
ATGAGAGGTAAGATCTTTAT+AGG + tig0019276:31418-31437 None:intergenic 30.0%
!! ACTTTTTCCTGAAAAATGAG+AGG + tig0019276:31433-31452 None:intergenic 30.0%
! TTTTCACTGAATTGATCATC+TGG - tig0019276:31479-31498 Msa1372280:exon 30.0%
TTTCACTGAATTGATCATCT+GGG - tig0019276:31480-31499 Msa1372280:exon 30.0%
TGAGAGAGAGTTGAATTAAA+TGG - tig0019276:31612-31631 Msa1372280:intron 30.0%
TAAAAACATCATCACCTTTC+GGG + tig0019276:31662-31681 None:intergenic 30.0%
GTAAAAACATCATCACCTTT+CGG + tig0019276:31663-31682 None:intergenic 30.0%
!! GGGATTGTTTTATGTTTTTG+TGG - tig0019276:31855-31874 Msa1372280:CDS 30.0%
! GCTATGTTTTACTTTCATCT+TGG - tig0019276:32143-32162 Msa1372280:intron 30.0%
! GTTTTACTTTCATCTTGGAT+TGG - tig0019276:32148-32167 Msa1372280:intron 30.0%
! TGGCGCCATATATTATATTT+TGG - tig0019276:32168-32187 Msa1372280:intron 30.0%
!! GGACTTTTTGCTATATTTGA+TGG - tig0019276:32500-32519 Msa1372280:intron 30.0%
CCTTCAAGATATTAGAAAAG+AGG + tig0019276:32565-32584 None:intergenic 30.0%
! CCTCTTTTCTAATATCTTGA+AGG - tig0019276:32562-32581 Msa1372280:intron 30.0%
! TCACAAGTTATGATTGGTTA+TGG - tig0019276:32658-32677 Msa1372280:intron 30.0%
ATTCATCTTGTCATTCTATG+AGG - tig0019276:33020-33039 Msa1372280:CDS 30.0%
AGCATAAGACTTCAATTTGA+TGG + tig0019276:33083-33102 None:intergenic 30.0%
TTATTAGCAAATACATCCAC+AGG + tig0019276:33140-33159 None:intergenic 30.0%
! CATTCTTCGTACATCTTTTA+TGG - tig0019276:33169-33188 Msa1372280:intron 30.0%
GCTTCACTTGTTTAGAAAAT+AGG - tig0019276:33341-33360 Msa1372280:CDS 30.0%
! GAAGTTATGGTTATTGGTTA+GGG + tig0019276:33466-33485 None:intergenic 30.0%
TCAATAAGAGTAGGAAGTTA+TGG + tig0019276:33479-33498 None:intergenic 30.0%
ACATTAACAAGCCAAAACTT+GGG + tig0019276:33813-33832 None:intergenic 30.0%
TACATTAACAAGCCAAAACT+TGG + tig0019276:33814-33833 None:intergenic 30.0%
! TTGTTAATGTAGAGGTACAT+TGG - tig0019276:33823-33842 Msa1372280:intron 30.0%
! CATTGATGTTAAATCCGTTT+TGG - tig0019276:33850-33869 Msa1372280:intron 30.0%
! CTGAATTTTACATGGGAAAA+AGG + tig0019276:34393-34412 None:intergenic 30.0%
! GCTTCAAACTGAATTTTACA+TGG + tig0019276:34401-34420 None:intergenic 30.0%
TATTGTGTGTGTGTGTATAT+TGG - tig0019276:34633-34652 Msa1372280:five_prime_UTR 30.0%
GCAAAACACAAATAAGGAAG+AGG + tig0019276:31176-31195 None:intergenic 35.0%
ATGGCTGCAAAACACAAATA+AGG + tig0019276:31182-31201 None:intergenic 35.0%
GGAAAGAATTTGTAAGCATC+TGG + tig0019276:31270-31289 None:intergenic 35.0%
GGTAAGATCTTTATAGGAAG+TGG + tig0019276:31412-31431 None:intergenic 35.0%
!! GATCTTACCTCTCATTTTTC+AGG - tig0019276:31423-31442 Msa1372280:three_prime_UTR 35.0%
TCATCTGAGTGAAAATTCAG+AGG - tig0019276:31515-31534 Msa1372280:CDS 35.0%
!!! TTCAGAGGTTTTGAAAGTTG+TGG - tig0019276:31530-31549 Msa1372280:CDS 35.0%
AGATATGAGCTTATGTAGCA+CGG - tig0019276:31932-31951 Msa1372280:intron 35.0%
ATGAAGCATAAACACAGACA+CGG + tig0019276:32071-32090 None:intergenic 35.0%
TGTCTGTGTTTATGCTTCAT+AGG - tig0019276:32071-32090 Msa1372280:intron 35.0%
AGCAAAAGCCATACTTAAGA+GGG + tig0019276:32403-32422 None:intergenic 35.0%
AAGCAAAAGCCATACTTAAG+AGG + tig0019276:32404-32423 None:intergenic 35.0%
! TATGGCTTTTGCTTAGTTGA+AGG - tig0019276:32410-32429 Msa1372280:intron 35.0%
! ATGGCTTTTGCTTAGTTGAA+GGG - tig0019276:32411-32430 Msa1372280:intron 35.0%
! TGCTATATTTGATGGACACT+CGG - tig0019276:32508-32527 Msa1372280:intron 35.0%
!! AGGTGTTTTTGTAAATAGGC+AGG + tig0019276:32545-32564 None:intergenic 35.0%
! AAGTTATGATTGGTTATGGC+AGG - tig0019276:32662-32681 Msa1372280:intron 35.0%
ATGATGCGTCTTTCAGTATT+GGG + tig0019276:32906-32925 None:intergenic 35.0%
GGTTTATTAAAGAACCTGGA+AGG + tig0019276:32956-32975 None:intergenic 35.0%
! GGTTTTCATCAACAATGGAA+AGG + tig0019276:33119-33138 None:intergenic 35.0%
ACTAAACATGGATTCCTACA+TGG + tig0019276:33283-33302 None:intergenic 35.0%
GATTAAACTGGGACTAAACA+TGG + tig0019276:33295-33314 None:intergenic 35.0%
TTCTAAACAAGTGAAGCTCA+GGG + tig0019276:33338-33357 None:intergenic 35.0%
TTTCTAAACAAGTGAAGCTC+AGG + tig0019276:33339-33358 None:intergenic 35.0%
ACTTGTTTAGAAAATAGGCC+TGG - tig0019276:33346-33365 Msa1372280:CDS 35.0%
AGGCAACACATAATAAATCC+AGG + tig0019276:33367-33386 None:intergenic 35.0%
!!! ATATCAAGTTGGGCTTTTTG+AGG + tig0019276:33387-33406 None:intergenic 35.0%
! GGAAGTTATGGTTATTGGTT+AGG + tig0019276:33467-33486 None:intergenic 35.0%
GAGTAGGAAGTTATGGTTAT+TGG + tig0019276:33472-33491 None:intergenic 35.0%
GGACTCAAATCAATAAGAGT+AGG + tig0019276:33488-33507 None:intergenic 35.0%
!! TATTGATTTGAGTCCTGCAT+TGG - tig0019276:33493-33512 Msa1372280:intron 35.0%
!! ATTCACAAAACCGACTTGTA+AGG + tig0019276:33567-33586 None:intergenic 35.0%
! TAGTCCAATAGATCTAAGGT+AGG + tig0019276:33608-33627 None:intergenic 35.0%
CAGATAGTCCAATAGATCTA+AGG + tig0019276:33612-33631 None:intergenic 35.0%
ACTATCTGTTATCAACCACT+TGG - tig0019276:33624-33643 Msa1372280:intron 35.0%
CAAAACTTGGGTTTGATGTT+AGG + tig0019276:33801-33820 None:intergenic 35.0%
! CTAACATCAAACCCAAGTTT+TGG - tig0019276:33799-33818 Msa1372280:intron 35.0%
!!! GTTTTGGCTTGTTAATGTAG+AGG - tig0019276:33815-33834 Msa1372280:intron 35.0%
TTCACAACACTCAACCAAAA+CGG + tig0019276:33867-33886 None:intergenic 35.0%
TCACTTGTGGATGTTATATC+AGG - tig0019276:33892-33911 Msa1372280:intron 35.0%
AGTATCTTGGTCAATATCGT+CGG + tig0019276:34013-34032 None:intergenic 35.0%
TGAAGCTCTTCGATTCAATT+AGG - tig0019276:34415-34434 Msa1372280:five_prime_UTR 35.0%
GTTGCGGAAATGAAGAGAAA+AGG + tig0019276:31366-31385 None:intergenic 40.0%
TCTTCATTTCCGCAACAGAA+CGG - tig0019276:31370-31389 Msa1372280:three_prime_UTR 40.0%
AGATCTTTATAGGAAGTGGG+TGG + tig0019276:31408-31427 None:intergenic 40.0%
GACACACCTTCAATCTAAAG+TGG + tig0019276:31958-31977 None:intergenic 40.0%
! CTTTAGATTGAAGGTGTGTC+TGG - tig0019276:31958-31977 Msa1372280:intron 40.0%
CACTATGACACTGATACATG+AGG - tig0019276:31993-32012 Msa1372280:intron 40.0%
ATGAGGCACATTCAAACTAC+TGG - tig0019276:32010-32029 Msa1372280:intron 40.0%
CACATTCAAACTACTGGTGT+CGG - tig0019276:32016-32035 Msa1372280:intron 40.0%
!! TTTTGGTTGTTGTACTGGTG+TGG - tig0019276:32185-32204 Msa1372280:intron 40.0%
! GCTATATTTGATGGACACTC+GGG - tig0019276:32509-32528 Msa1372280:intron 40.0%
! GGTTATGGCAGGAGAATTTT+TGG - tig0019276:32673-32692 Msa1372280:intron 40.0%
TTCGATCATCGAAGCTTATG+AGG - tig0019276:32710-32729 Msa1372280:intron 40.0%
TCTTTCTCACAGTCCTGATT+TGG - tig0019276:32749-32768 Msa1372280:intron 40.0%
CTTTCTCACAGTCCTGATTT+GGG - tig0019276:32750-32769 Msa1372280:intron 40.0%
! TTTCTCACAGTCCTGATTTG+GGG - tig0019276:32751-32770 Msa1372280:intron 40.0%
GATGATGCGTCTTTCAGTAT+TGG + tig0019276:32907-32926 None:intergenic 40.0%
AGACGCATCATCGAGAATAA+AGG - tig0019276:32915-32934 Msa1372280:CDS 40.0%
! CCACAGGTTTTCATCAACAA+TGG + tig0019276:33124-33143 None:intergenic 40.0%
! CCATTGTTGATGAAAACCTG+TGG - tig0019276:33121-33140 Msa1372280:intron 40.0%
AGAATCGTCTTGAGAAGTCA+TGG - tig0019276:33256-33275 Msa1372280:CDS 40.0%
GTCCTGCATTGGATGTTATA+AGG - tig0019276:33504-33523 Msa1372280:intron 40.0%
! AATAGATCTAAGGTAGGCTC+TGG + tig0019276:33602-33621 None:intergenic 40.0%
AGAGCCTACCTTAGATCTAT+TGG - tig0019276:33601-33620 Msa1372280:intron 40.0%
GTTGTGAACTTTCTCACTTG+TGG - tig0019276:33879-33898 Msa1372280:intron 40.0%
GTATCTTGGTCAATATCGTC+GGG + tig0019276:34012-34031 None:intergenic 40.0%
GTATCAAGAGCTCAGTATCT+TGG + tig0019276:34026-34045 None:intergenic 40.0%
! CTCTTGATACTTGCTAGTGA+TGG - tig0019276:34036-34055 Msa1372280:intron 40.0%
! TACTTGCTAGTGATGGTCTA+TGG - tig0019276:34043-34062 Msa1372280:intron 40.0%
TTGCAATATCAACTGCCTCT+TGG + tig0019276:34187-34206 None:intergenic 40.0%
! AGTTGTTTAGCTGCCTTTTG+TGG + tig0019276:34224-34243 None:intergenic 40.0%
AGCTAAACAACTAACTGCAG+AGG - tig0019276:34232-34251 Msa1372280:five_prime_UTR 40.0%
AGCTCTTCGATTCAATTAGG+TGG - tig0019276:34418-34437 Msa1372280:five_prime_UTR 40.0%
AGCAGTGTGAAGATGAACAT+TGG - tig0019276:34518-34537 Msa1372280:five_prime_UTR 40.0%
TGGGAATTGGAATACATGAG+TGG - tig0019276:34556-34575 Msa1372280:five_prime_UTR 40.0%
AGCTTGAGTAACTTGATCCT+CGG - tig0019276:34583-34602 Msa1372280:five_prime_UTR 40.0%
AGTCGCTTATATTATAGCCG+AGG + tig0019276:34603-34622 None:intergenic 40.0%
TTATAGGAAGTGGGTGGTCA+CGG + tig0019276:31402-31421 None:intergenic 45.0%
TTGCTTCAACTCTTCCCGAA+AGG - tig0019276:31645-31664 Msa1372280:intron 45.0%
CAGCAACTTCATAACAGTCG+TGG - tig0019276:31882-31901 Msa1372280:intron 45.0%
ATAAACACAGACACGGACAC+CGG + tig0019276:32064-32083 None:intergenic 45.0%
!!! CTTGCAACGTTTTAGGGTGT+TGG - tig0019276:32332-32351 Msa1372280:intron 45.0%
GTTGGAGGACGTTCTTTATG+CGG - tig0019276:32350-32369 Msa1372280:intron 45.0%
CATACTTAAGAGGGCCTTGA+AGG + tig0019276:32394-32413 None:intergenic 45.0%
CTTCAAGGCCCTCTTAAGTA+TGG - tig0019276:32392-32411 Msa1372280:intron 45.0%
GCGAAAATCGTCCAGTTCAA+CGG - tig0019276:32467-32486 Msa1372280:intron 45.0%
CGAAAATCGTCCAGTTCAAC+GGG - tig0019276:32468-32487 Msa1372280:intron 45.0%
GCTTCGATGATCGAACTGTT+TGG + tig0019276:32705-32724 None:intergenic 45.0%
AGCTTATGAGGCAACTGATC+AGG - tig0019276:32722-32741 Msa1372280:intron 45.0%
CGCATCATCGAGAATAAAGG+CGG - tig0019276:32918-32937 Msa1372280:CDS 45.0%
!! GGTTTTGTCTCAAACCTTCC+AGG - tig0019276:32939-32958 Msa1372280:CDS 45.0%
TGAGAAGTCATGGACCATGT+AGG - tig0019276:33266-33285 Msa1372280:CDS 45.0%
! GGCCTTATAACATCCAATGC+AGG + tig0019276:33509-33528 None:intergenic 45.0%
GTATCTCGCACCTTACAAGT+CGG - tig0019276:33554-33573 Msa1372280:intron 45.0%
! ATATCAGAAGCGTGACCAAG+TGG + tig0019276:33642-33661 None:intergenic 45.0%
GGTGATGTTGCAAGAGTGAA+TGG - tig0019276:33913-33932 Msa1372280:intron 45.0%
! ATATCGTCGGGTTTTACGTC+TGG + tig0019276:34000-34019 None:intergenic 45.0%
TGCTAGTGATGGTCTATGGA+AGG - tig0019276:34047-34066 Msa1372280:intron 45.0%
! TCAGGTAATGGCAAACCAAG+AGG - tig0019276:34169-34188 Msa1372280:five_prime_UTR 45.0%
!! AGGCATTGAAAAGAGAGAGC+AGG - tig0019276:34252-34271 Msa1372280:five_prime_UTR 45.0%
! GGCATTGAAAAGAGAGAGCA+GGG - tig0019276:34253-34272 Msa1372280:five_prime_UTR 45.0%
CTTGAAGCGAACTACAATGC+AGG + tig0019276:34288-34307 None:intergenic 45.0%
! CGGATTTTGCCGTTCTGTTG+CGG + tig0019276:31382-31401 None:intergenic 50.0%
! GCACGGCCACTTTAGATTGA+AGG - tig0019276:31949-31968 Msa1372280:intron 50.0%
GCGTGTCTGTGTAAGTGACA+TGG - tig0019276:32038-32057 Msa1372280:intron 50.0%
GTCTGTGTAAGTGACATGGC+CGG - tig0019276:32042-32061 Msa1372280:intron 50.0%
!!! GCAACGTTTTAGGGTGTTGG+AGG - tig0019276:32335-32354 Msa1372280:intron 50.0%
! TCTTTATGCGGCTCTGGCAA+AGG - tig0019276:32362-32381 Msa1372280:intron 50.0%
AGGGACACAAGACCACACAA+TGG - tig0019276:32430-32449 Msa1372280:intron 50.0%
GACACAAGACCACACAATGG+AGG - tig0019276:32433-32452 Msa1372280:intron 50.0%
CGTGAAAGTCCTCCATTGTG+TGG + tig0019276:32445-32464 None:intergenic 50.0%
ATTCACGAGCAGTTCTGTCG+AGG - tig0019276:32844-32863 Msa1372280:CDS 50.0%
AGTTCTGTCGAGGAAAGGTG+TGG - tig0019276:32854-32873 Msa1372280:CDS 50.0%
ATGGACAGCTTGCAGTTGCT+CGG - tig0019276:33932-33951 Msa1372280:intron 50.0%
CAGATCGCAAGTGTGACTTC+AGG + tig0019276:33975-33994 None:intergenic 50.0%
ACAGTCGTGGATGCTGTGTG+AGG - tig0019276:31895-31914 Msa1372280:intron 55.0%
! TTGAAGGTGTGTCTGGTGCG+CGG - tig0019276:31965-31984 Msa1372280:intron 55.0%
GGACGTTCTTTATGCGGCTC+TGG - tig0019276:32356-32375 Msa1372280:intron 55.0%
GGCAAAGGCAAGAGCCTTCA+AGG - tig0019276:32377-32396 Msa1372280:intron 55.0%
CAGTTCAACGGGCGAGAGTT+GGG - tig0019276:32479-32498 Msa1372280:intron 55.0%
CGAGCAGTTCTGTCGAGGAA+AGG - tig0019276:32849-32868 Msa1372280:CDS 55.0%
TGGACAGCTTGCAGTTGCTC+GGG - tig0019276:33933-33952 Msa1372280:intron 55.0%
CTTGCAGTTGCTCGGGCATT+CGG - tig0019276:33940-33959 Msa1372280:intron 55.0%
CCAACTCTCGCCCGTTGAAC+TGG + tig0019276:32481-32500 None:intergenic 60.0%
CCAGTTCAACGGGCGAGAGT+TGG - tig0019276:32478-32497 Msa1372280:intron 60.0%
!! CACAGTCCTGATTTGGGGCG+TGG - tig0019276:32756-32775 Msa1372280:intron 60.0%
!! AGTCCTGATTTGGGGCGTGG+AGG - tig0019276:32759-32778 Msa1372280:intron 60.0%
GATCCTCCACGCCCCAAATC+AGG + tig0019276:32765-32784 None:intergenic 60.0%
! TTTGGGGCGTGGAGGATCGA+CGG - tig0019276:32767-32786 Msa1372280:intron 60.0%
Chromosome Type Strat End Strand Name
tig0019276 gene 31113 34682 31113 ID=Msa1372280;Name=Msa1372280
tig0019276 mRNA 31113 34682 31113 ID=Msa1372280-mRNA-1;Parent=Msa1372280;Name=Msa1372280-mRNA-1;_AED=0.25;_eAED=0.25;_QI=518|1|1|1|1|1|5|372|281
tig0019276 exon 31113 31622 31113 ID=Msa1372280-mRNA-1:exon:2769;Parent=Msa1372280-mRNA-1
tig0019276 exon 31727 31881 31727 ID=Msa1372280-mRNA-1:exon:2768;Parent=Msa1372280-mRNA-1
tig0019276 exon 32835 33111 32835 ID=Msa1372280-mRNA-1:exon:2767;Parent=Msa1372280-mRNA-1
tig0019276 exon 33209 33448 33209 ID=Msa1372280-mRNA-1:exon:2766;Parent=Msa1372280-mRNA-1
tig0019276 exon 34129 34682 34129 ID=Msa1372280-mRNA-1:exon:2765;Parent=Msa1372280-mRNA-1
tig0019276 five_prime_UTR 34165 34682 34165 ID=Msa1372280-mRNA-1:five_prime_utr;Parent=Msa1372280-mRNA-1
tig0019276 CDS 34129 34164 34129 ID=Msa1372280-mRNA-1:cds;Parent=Msa1372280-mRNA-1
tig0019276 CDS 33209 33448 33209 ID=Msa1372280-mRNA-1:cds;Parent=Msa1372280-mRNA-1
tig0019276 CDS 32835 33111 32835 ID=Msa1372280-mRNA-1:cds;Parent=Msa1372280-mRNA-1
tig0019276 CDS 31727 31881 31727 ID=Msa1372280-mRNA-1:cds;Parent=Msa1372280-mRNA-1
tig0019276 CDS 31485 31622 31485 ID=Msa1372280-mRNA-1:cds;Parent=Msa1372280-mRNA-1
tig0019276 three_prime_UTR 31113 31484 31113 ID=Msa1372280-mRNA-1:three_prime_utr;Parent=Msa1372280-mRNA-1
Gene Sequence

>Msa1372280

ATGGATAGTCTCTGTTGCTTCAACTCTTCCCGAAAGGGTGTTGGAGGACGTTCTTTATGCGGCTCTGGCAAAGGCAAGAGCCTTCAAGGCCCTCTTAAGTATGGCTTTTGCTTAGTTGAAGGGACACAAGACCACACAATGGAGGACTTTCACGTTGCGAAAATCGTCCAGTTCAACGGGCGAGAGTTGGGACTTTTTGCTATATTTGATGGACACTCGGGTGACACTGTGCCTGCCTATTTACAAAAACACCTCTTTTCTAATATCTTGAAGGAAGAGAATTTTTGGACTGATCCAAACAGTTCGATCATCGAAGCTTATGAGGCAACTGATCAGGCTATTCTTTCTCACAGTCCTGATTTGGGGCGTGGAGGATCGACGGCAGTGACTGCCATTCTAATAAATAATCAGAAATTATGGATAGCAAATGTTGGAGATTCACGAGCAGTTCTGTCGAGGAAAGGTGTGGCTATACAGATGAGTATTGATCACGAGCCCAATACTGAAAGACGCATCATCGAGAATAAAGGCGGTTTTGTCTCAAACCTTCCAGGTGATGTTGCAAGAGTGAATGGACAGCTTGCAGTTGCTCGGGCATTCGGAGATAGAAACCTGAAGTCACACTTGCGATCTGATCCAGACGTAAAACCCGACGATATTGACCAAGATACTGAGCTCTTGATACTTGCTAGTGATGGTCTATGGAAGGTAATGGCAAACCAAGAGGCAGTTGATATTGCAATAAAGATAAAAGATCCACAAAAGGCAGCTAAACAACTAACTGCAGAGGCATTGAAAAGAGAGAGCAGGGATGACATTTCCTGCATTGTAGTTCGCTTCAAGTGA
Protein sequence

>Msa1372280

MDSLCCFNSSRKGVGGRSLCGSGKGKSLQGPLKYGFCLVEGTQDHTMEDFHVAKIVQFNGRELGLFAIFDGHSGDTVPAYLQKHLFSNILKEENFWTDPNSSIIEAYEATDQAILSHSPDLGRGGSTAVTAILINNQKLWIANVGDSRAVLSRKGVAIQMSIDHEPNTERRIIENKGGFVSNLPGDVARVNGQLAVARAFGDRNLKSHLRSDPDVKPDDIDQDTELLILASDGLWKVMANQEAVDIAIKIKDPQKAAKQLTAEALKRESRDDISCIVVRFK*